Aflatoxin Destruction in Corn Using Sodium Bisulfite, Sodium Hydroxide and Aqueous Ammonia

1980 ◽  
Vol 43 (7) ◽  
pp. 571-574 ◽  
Author(s):  
KURT E. MOERCK ◽  
PAUL McELFRESH ◽  
ALAN WOHLMAN ◽  
BARNEY W. HILTON
Keyword(s):  
Moisture Content ◽  
Ambient Temperature ◽  
Sodium Hydroxide ◽  
Aflatoxin B1 ◽  
Aqueous Ammonia ◽  
Sodium Bisulfite ◽  
Total Aflatoxin ◽  
Yellow Corn ◽  
Dent Corn ◽  
Aflatoxin B2

Naturally contaminated yellow dent corn containing 235 ppb of aflatoxin B1 and B2 was adjusted to a moisture content of 20% and then treated for 24 h at ambient temperature with NaHS03, NaOH or aqueous NH3 at 0.5%, 1.0%, or 2.0% concentrations. All treatments were effective in reducing aflatoxin B1 and B2 levels. Sodium bisulfite was more effective in destroying aflatoxins than were NaOH or aqueous NH3 at 0.5% and 1.0% concentrations, while NaOH and aqueous NH3 were more effective than bisulfite at 2.0% concentration. Subjecting yellow corn samples to either NaHSO3, NaOH or aqueous NH3 at 2.0% concentrations reduced aflatoxin B1 and B2 levels to below the FDA guideline of 20 ppb total aflatoxin. Sodium bisulfite was also effective in reducing the levels of aflatoxins in a white dent corn sample containing 81 ppb of aflatoxin B1 and 12 ppb of aflatoxin B2. Results suggest that NaHSO3, NaOH or aqueous NH3 can be used to effectively destroy aflatoxins in corn and possibly other agricultural commodities.

scholarly journals Changes in Fungal Population and Aflatoxin Levels and Assessment of Major Aflatoxin Types in Stored Peanuts (Arachis hypogaea Linnaeus)

2013 ◽  
Vol 2 (5) ◽  
pp. 10 ◽  
Author(s):  
John Maina Wagacha ◽  
Charity K. Mutegi ◽  
Maria E. Christie ◽  
Lucy W. Karanja ◽  
Job Kimani
Keyword(s):  
Moisture Content ◽  
Aflatoxin B1 ◽  
Fungal Pathogens ◽  
Aflatoxin Contamination ◽  
Fungal Population ◽  
Total Aflatoxin ◽  
Regulatory Standards ◽  
Polyethylene Bags ◽  
Jute Bags ◽  
The Eu

<p>Peanut kernels of Homabay Local, Valencia Red, ICGV-SM 12991 and ICGV-SM 99568 cultivars were stored for six months in jute, polypropylene and polyethylene bags to assess the effect of storage bags, temperature and R.H. on fungal population and aflatoxin contamination. Moisture content (M.C.), fungal population and aflatoxin levels were determined before storage and after every 30 days during storage. Isolates of <em>Aspergillus flavus</em> and <em>A. parasiticus</em> were assayed for production of aflatoxin B1, B2, G1 and G2. The correlation between MC, population of <em>A. flavus</em> and <em>A. parasiticus</em> and aflatoxin levels in peanuts was also determined. Six fungal pathogens were commonly isolated from the peanut samples and occurred as follows in decreasing order: <em>Penicillium</em> spp. (106.6 CFU/g), <em>A. flavus</em> L-strain (4.8 CFU/g), <em>A. flavus</em> S-strain (2.9 CFU/g), <em>A. niger </em>(2.6 CFU/g), <em>A. parasiticus </em>(1.7 CFU/g) and <em>A. tamarii </em>(0.2 CFU/g). The overall population of <em>A. flavus</em> L-strain was 66% higher than that of <em>A. flavus</em> S-strain. Ninety one percent of <em>A. flavus</em> and <em>A. parasiticus</em> isolates produced at least one of the four aflatoxin types assayed, with 36% producing aflatoxin B1. Total aflatoxin levels ranged from 0 - 47.8 µg/kg with samples stored in polyethylene and jute bags being the most and least contaminated, respectively. Eighty nine percent and 97% of the peanut samples met the EU (? 4 µg/kg) and Kenyan (? 10 µg/kg) regulatory standards for total aflatoxin, respectively. Peanuts should be adequately dried to safe moisture level and immediately packaged in a container - preferably jute bags - which will not promote critical increases in fungal population and aflatoxin contamination.</p>

Destruction of Aflatoxin in Corn with Sodium Bisulfite1,2,3

1982 ◽  
Vol 45 (14) ◽  
pp. 1287-1291 ◽  
Author(s):  
WINSTON M. HAGLER ◽  
JAMES E. HUTCHINS ◽  
PAT B. HAMILTON
Keyword(s):  
Adverse Effect ◽  
Aflatoxin B1 ◽  
Weight Basis ◽  
Sodium Bisulfite ◽  
The Other ◽  
Feed Consumption ◽  
Complete Destruction ◽  
Plastic Bags ◽  
Wet Weight ◽  
Aflatoxin B2

The ability of sodium bisulfite to destroy aflatoxins B1 and B2 in naturally contaminated corn containing about 2350 ppb of B1 and 450 ppb of B2 was investigated. Under certain conditions, complete destruction of aflatoxin B1 was achieved. Aflatoxin B2, on the other hand, was resistant to sodium bisulfite and never over about 50% was destroyed. Moisture, sodium bisulfite level, time, as well as temperature had significant effects on aflatoxin degradation. Moisture levels of over 50% (wet weight basis) had a strongly adverse effect on the aflatoxin-bisulfite reaction. The most effective treatment involved soaking whole-kernel corn in a 10% sodium bisulfite solution for 72 h, removing the solution and incubating the corn in sealed plastic bags at 50°C. Complete destruction of aflatoxin B1 was achieved by 21 d. Sodium bisulfite exhibited antimicrobial activity in corn comparable to that of propionic acid, indicating possible utility as an effective mold inhibitor in stored corn at up to 40% moisture. Feed consumption by young chickens was unaffected until feed containing over 20 g of sodium bisulfite/kg was presented.

scholarly journals Aflatoxins and Moisture Levels in Edible Oils Produced in Burkina Faso

2020 ◽  
pp. 1-12
Author(s):  
Souleymane Zio ◽  
Isaac Dembele ◽  
Raoul Sylvain Bazoin Bazié ◽  
Abel Tankoano ◽  
François Tapsoba ◽  
...  
Keyword(s):  
Moisture Content ◽  
Burkina Faso ◽  
Aflatoxin B1 ◽  
Edible Oils ◽  
Consumer Health ◽  
Health It ◽  
Bobo Dioulasso ◽  
Surrounding Areas ◽  
Aflatoxin B2 ◽  
The Impact

Aim: The study aim was to assess aflatoxin and moisture levels in edible oils produced and consumed in Burkina Faso to know the impact on consumer health. Methodology: A total of 61 samples of refined cottonseeds oils and crude peanut oils were collected from Ouagadougou, Bobo Dioulasso and surrounding areas. Aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1) and aflatoxin G2 (AFG2) were determined by HPLC and moisture by differential weighing after oven drying. Results: The moisture content of peanut oils were ranged from 0.06 to 0.18% and cottonseeds oils from 0.02 to 0.17%. The moisture average is 0.13% for peanut oils and 0.08% for cottonseeds oils (P<0.05). The moisture of all oils is lower and conform to the Codex Alimentarius standard. AFB1, AFB2, AFG1 and AFG2 were identified in 86.89% of the oil samples analyzed. The proportion of samples contaminated with AFB1 is 57.38%, 59.02% for AFB2, 42.62% for AFG1 and 65.57% for AFG2. The AFB1 average of peanut oils is 6.21 ng/g while that of cottonseeds oils is 0.03 ng/g. The AFB2 average of peanut oils is 0.89 ng/g against 0.04 ng/g for cottonseeds oils (P<0.05). The AFG1 average of peanut oils is 0.54 ng/g and 0.08 ng/g for cottonseeds oils (P<0.05). The AFG2 average of peanut oils was 0.66 ng/g against 0.64 ng/g for cottonseeds oils. AFG2 had the highest proportion of all oils while AFB1 has the highest concentration and proportion in peanut oils. The 72.13% samples analyzed in this study comply with the European Community standard for aflatoxin B1 level maximum in oilseeds. Conclusion: Aside from the moisture content that comply with the standard, aflatoxins are present at varying levels and can negatively impact the consumer health. It is important to strengthen the monitoring and production system in order to have quality oil.

Improvement in the Fock test for determining the reactivity of dissolving pulp

TAPPI Journal ◽  
2013 ◽  
Vol 12 (11) ◽  
pp. 21-26 ◽  
Author(s):  
CHAO TIAN ◽  
LINQIANG ZHENG ◽  
QINGXIAN MIAO ◽  
CHRIS NASH ◽  
CHUNYU CAO ◽  
...  
Keyword(s):  
Moisture Content ◽  
Sodium Hydroxide ◽  
Constant Temperature ◽  
Carbon Disulfide ◽  
Testing Procedure ◽  
Dissolving Pulp ◽  
Water Bath ◽  
Testing Conditions ◽  
Pulp Sample ◽  
Fock Reactivity

The Fock test is widely used for assessing the reactivity of dissolving pulp. The objective of this study was to modify the method to improve the repeatability of the test. Various parameters that affect the repeatability of the Fock test were investigated. The results showed that Fock reactivity is dependent on testing conditions affecting the xanthation between cellulose and carbon disulfide, such as the moisture content of the pulp sample, sodium hydroxide (NaOH) concentration, xanthation temperature, carbon disulfide dosage, and xanthation time. The repeatability of the test was significantly improved using the following modified testing procedure: air dried sample in the constant temperature/humidity room, xanthation temperature of 66°F (19°C) in a water bath, xanthation time of 3 h, NaOH concentration of 9% (w/w), and 1.3 mL carbon disulfide.

CATFISH DRYING (Clarias Batraclus) USING ‘TEKO BERSAYAP’ SOLAR DRYER

2012 ◽  
Vol 2 (1) ◽  
pp. 14-20
Author(s):  
Yuwana Yuwana
Keyword(s):  
Relative Humidity ◽  
Moisture Content ◽  
Ambient Temperature ◽  
Drying Rate ◽  
Drying Process ◽  
Drying Time ◽  
Ambient Relative Humidity ◽  
Solar Dryer

Experiment on catfish drying employing ‘Teko Bersayap’ solar dryer was conducted. The result of the experiment indicated that the dryer was able to increase ambient temperature up to 44% and decrease ambient relative humidity up to 103%. Fish drying process followed equations : KAu = 74,94 e-0,03t for unsplitted fish and KAb = 79,25 e-0,09t for splitted fish, where KAu = moisture content of unsplitted fish (%), KAb = moisture content of splitted fish (%), t = drying time. Drying of unsplitted fish finished in 43.995 hours while drying of split fish completed in 15.29 hours. Splitting the fish increased 2,877 times drying rate.

scholarly journals Occurrence of mycotoxins in selected agricultural and commercial products available in eastern Poland

2021 ◽  
Vol 19 (1) ◽  
pp. 653-664
Author(s):  
Grażyna Kowalska ◽  
Radosław Kowalski
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Extraction Method ◽  
Maximum Allowable Concentration ◽  
Commercial Products ◽  
Ms Analysis ◽  
Rye Flour ◽  
Three Samples ◽  
Aflatoxin B2

Abstract The objective of this study was the estimation of the content of 13 mycotoxins (diacetoxyscirpenol, T-2 toxin, HT-2 toxin, nivalenol, deoxynivalenol, 3-acetyldeoxynivalenol, fusarenone X, aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, ochratoxin A, and zearalenone) in various products from the eastern part of Poland. The content of mycotoxins in the analysed samples was assayed using the extraction method combined with HPLC-MS/MS analysis. We found mycotoxins in 25 of the 92 samples tested (27%). Contamination with mycotoxins was noted most frequently in samples of cereals – 56% – and also in samples of flour and cocoa, in which a content of mycotoxins was noted in 24 and 16% of the samples, respectively. The most frequently identified were the following – deoxynivalenol detected in 18 samples (72%), zearalenone detected in eight samples (32%), toxin HT-2 detected in four samples (16%), ochratoxin A identified in three samples (12%), and toxin T-2 detected in one sample (4%). In one analysed sample of mixed flour and in one analysed sample of wheat and rye flour, the maximum allowable concentration was exceeded in the case of two identified mycotoxins – deoxynivalenol (2,250 μg/kg) and ochratoxin A (15.6 and 17.1 μg/kg).

Corn silage as a source of aflatoxin B1 in feed for dairy cattle

2021 ◽  
Vol 26 (4) ◽  
pp. 2759-2764
Author(s):  
DRAGAN GLAMOČIĆ ◽  
MIROSLAVA POLOVINSKI HORVATOVIĆ ◽  
IGOR JAJIĆ ◽  
SAŠA KRSTOVIĆ ◽  
MIRKO IVKOVIĆ ◽  
...  
Keyword(s):  
Moisture Content ◽  
Dairy Cattle ◽  
Aflatoxin B1 ◽  
Corn Silage ◽  
Aflatoxin M1 ◽  
Fresh Matter ◽  
Whole Plant ◽  
Corn Grain

Nutrition of dairy cattle is based on two components, concentrates and forages. The main forages in Vojvodina, north province of Serbia is silage made from the whole plant of corn. After the outbreak of aflatoxin B1 in corn in 2012, the occurrence of aflatoxin B1 in corn as a source of contamination of aflatoxin M1 in milk was very broadly investigated. There is no data regarding the occurrence of aflatoxin B1 in silage and how much silage can contribute to the overall intake of aflatoxin B1 in this region. This work is an attempt to estimate how much silage, in condition and practice used in Vojvodina, contributes to the intake of aflatoxin B1, and consequently aflatoxin M1 in milk. In total, 82 samples of corn grain and 72 samples of corn silage were analyzed on the occurrence of aflatoxin B1 during 2017-2018 period. Aflatoxin B1 was found in 13.41% of corn samples in the range from 6.82 to 187.5 ppb (average 63.5 ppb). All positive samples were from 2017, while no positive samples were found during 2018. Incidence of aflatoxin B1 in silage was 54.17% in the range of 3.5-58.0 ppb (12% moisture content) or 0.95-16.1 ppb in the fresh matter. Results suggest that silage can be a significant factor to overall intake of aflatoxin B1 and that further research is needed.

scholarly journals Grain Processing Methods’ Effectiveness to Eliminate Mycotoxins: An Overview

2021 ◽  
Vol 33 (10) ◽  
pp. 2267-2275
Author(s):  
Chinaza Godswill Awuchi ◽  
Erick Nyakundi Ondari ◽  
Chigozie E. Ofoedu ◽  
James S. Chacha ◽  
Waheed A. Rasaq ◽  
...  
Keyword(s):  
Citric Acid ◽  
Sodium Hydroxide ◽  
Cold Plasma ◽  
Detection Limits ◽  
Sodium Bisulfite ◽  
Plasma Cleaning ◽  
Processing Methods ◽  
Chemical Treatments ◽  
Grain Processing ◽  
Processing Techniques

Mycotoxins like aflatoxins, ochratoxins, patulin, citrinin, deoxynivalenol (DON), fumonisins, trichothecenes, etc., produced by molds (Aspergillus avus, A. parasiticus, Penicillin spp, etc.) could occupy such grains as peanuts, millet, sunower, cassava, beans, sesame, sorghum, maize, etc. In order to either eliminate and or reduce the levels of mycotoxins, as well as detection limits, there are a number of grain processing techniques that have been reported in the literature. In this article, an overview of the effectiveness of grain processing methods to eliminate mycotoxins was performed. Specifically, the grain processing techniques considered include: extrusion, alkaline cooking, flaking, roasting, frying, baking, milling, cooking, cold plasma, cleaning, trimming, sorting, as well as chemical treatments like the use of sodium hydroxide, citric acid, sodium bisulfite and ammonia. Overall, while most methods seem effective in reducing some mycotoxins over others, more studies are required to identify how these methods particularly their combinations would elevate the removal/degradation of mycotoxins in grains, particularly to safer levels.

scholarly journals Strength Attainment of Geopolymer Concrete with GGBS at Ambient Curing

2019 ◽  
Vol 9 (2S2) ◽  
pp. 56-59
Keyword(s):  
Tensile Strength ◽  
Ambient Temperature ◽  
Sodium Hydroxide ◽  
Cross Sections ◽  
Strength Properties ◽  
Industrial Wastes ◽  
Mix Design ◽  
Geopolymer Concrete ◽  
Ambient Curing ◽  
Test Result

Geopolymer concrete plays a major role in concrete industry by replacing cement and using the industrial wastes. In this study, the cement is completely replaced by GGBS and strength properties are analyzed. An M30 mix design is prepared and the specimens are cast and tested. For this, sodium hydroxide and sodium silicate are used as activator and its ratio is fixed as 1:2.5. Sodium hydroxide of 12 molarity, 550kg/m3 of GGBS is used in the study. Admixture La Hypercrete S25 (HTS code 38244090) is added in the mix by 1% of weight of GGBS to obtain the required workability. For compression study, cubes in 100 mm size are cast. Cylinders with 100mm dia and 200mm height are tested for splitting tensile strength and beam specimens of 500mm long and 100mm cross sections were cast for determining the flexure behaviour. The beams are subjected to ambient curing and tested at 3, 7, 14, 28 and 56 days. The test result shows that there is a gradual increment in all the strengths from 3 to 56 days and it proves that geopolymer concrete with GGBS cured at ambient temperature performs well in the strength properties.

scholarly journals Aflatoxigenic contamination of freshly harvested white maize (Zea mays L.) from some selected Ugandan districts

2019 ◽  
Author(s):  
Timothy Omara
Keyword(s):  
Zea Mays ◽  
Moisture Content ◽  
Ochratoxin A ◽  
Zea Mays L ◽  
Lake Victoria ◽  
Total Aflatoxin ◽  
Strategic Adaptation ◽  
Maize Varieties ◽  
Agroecological Zones

The moisture content and total aflatoxin (AF) content of 27 samples of freshly harvested white maize (Zea mays L.) from Mubende (n = 3), Ibanda (n = 3), Jinja (n = 3), Mayuge (n = 3) , Buikwe (n = 3), Hoima (n = 3), Mpigi (n = 3), Masindi (n = 3) and Bugiri (n = 3) districts of Uganda representing the agroecological zones: Lake Victoria crescent, Western Highlands, South East and Lake Albert Crescent were determined in the second season harvest of January 2019 to March 2019. Moisture content ranged from 12.9 to 18.8% (mean moisture content varied from 13.9±0.35-17.2±1.55%) with the highest moisture recorded in maize from Ibanda. The highest mean AF contamination of 11.0±3.01 μg/kg was recorded in maize from Hoima while the lowest AF content of 3.8±1.30 μg/kg was recorded in maize from Mpigi. Despite the fact that all the samples had detectable aflatoxins, none of the maize samples had aflatoxin greater than WHO regulatory limit of 20 μg/kg. White maize in Uganda are precontaminated by aflatoxins prior to harvest. Whereas the spectre of aflatoxigenic contamination of foods remains a ticklish challenge to address, strategic adaptation and deployment of appropriate interventions can help secure a safe harvest. Farmers should plant maize varieties with established maturity periods to ensure timely harvesting. Further research should assess the presence of other mycotoxins as zearalenone, sterigmatocystin, ochratoxin A, citrinin, vomitoxin and diacetoxyscirpenol that may co-occur with aflatoxins in freshly harvested maize.

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