Analisis Jumlah Coliform dan Faecal Coli (MPN) pada Daging Sapi dan Ayam di Kota Manokwari

2020 ◽  
Vol 10 (2) ◽  
pp. 113
Author(s):  
Abdul Rahman Ollong ◽  
John Arnold Palulungan ◽  
Rizki Arizona
Keyword(s):  
Broiler Chicken ◽  
Pathogenic Bacteria ◽  
Microbial Growth ◽  
Chicken Meat ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Cross Contamination ◽  
Eligibility Criteria ◽  
Probable Number ◽  
Traditional Market

Abstract Beef and chicken meat are potential as a medium for microbial growth such as coliform bacteria since it has a high nutrients content. Coliform bacteria in a given amount could be used as a hygienic indicator and a sign for the presence of pathogenic bacteria. The aimed of this research was to determine the amount of coliform and Escherichia coli on fresh beef and broiler chicken. The bacterial tests included qualitative tests as presumptive test, confirmed test, completed test whereas quantitavely test as Most Probable Number (MPN) to test the number of coliform and faecal coli. The results showed that pH in beef meat is normal and chicken meat shows under normal pH (pH in beef anf chicken meat are  5,7 – 5,98).   Coliform and faecal coli bacteria was detected in 100% of samples had MPN values > 1 MPN/g. It means that the value do not meet eligibility criteria consumption. The high number of Coliform and faecal coli contamination in beef and chicken meat were supposed in relation with market sanitation, cross contamination with other food, low knowledge about hygiene.  Keywords: Number of coliform; Beef and chicken; Traditional market   Abstrak Daging sapi dan ayam memiliki kandungan nutrisi yang tinggi sehingga menjadi media yang baik untuk pertumbuhan mikroba. Salah satu jenis bakteri yang sering mengkontaminasi yaitu coliform dan faecal coli yang merupakan indikator higienis dan sebagai tanda keberadaan bakteri patogen. Penelitian ini bertujuan untuk menghitung jumlah coliform dan faecal coli pada sampel daging sapi dan ayam yang dijual di pasar traditional kota Manokwari menggunakan uji kualitatif yaitu uji presumptive test, confirmed test, completed test sedangkan kuantitatif yaitu Most Probable Number (MPN) untuk menguji jumlah coliform dan faecal coli. Hasil penelitian menunjukkan bahwa nilai pH daging sapi dalam kisaran normal dan pH ayam tergolong rendah yang semuanya pada kisaran 5,7 – 5,98; dan  100% sampel daging sapi, ayam dan pedagang daging terkontaminasi bakteri coliform dan faecal coli dengan nilai MPN yang tidak memenuhi kriteria kelayakan konsumsi, yakni > 1 MPN/g. Tingginya cemaran coliform dan faecal coli pada daging sapi dan ayam terkait dengan faktor kualitas sanitasi pasar, kontaminasi silang dengan bahan makanan lain dan pengetahuan higienitas yang rendah. Kata kunci : Jumlah coliform dan faecal coli; Daging sapi dan ayam; Pasar Tradisional

Analysis of Coliform and Colifecal Total Pollution Test on Various Types of Drinking Water Using the MPN (Most Probable Number) Method

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Wanda Aulya ◽  
Fadhliani Fadhliani ◽  
Vivi Mardina
Keyword(s):  
Drinking Water ◽  
Fecal Coliform ◽  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Inspection Method ◽  
Sample Number ◽  
Probable Number ◽  
Fecal Coliform Bacteria

Water is the main source for life and also the most severe substance caused by pollution. The mandatory parameters for determining microbiological quality of drinking water are total non-fecal Coliform bacteria and Coliform fecal (Escherichia coli). Coliform bacteria are a group of microorganisms commonly used as indicators, where these bacteria can be a signal to determine whether a water source has been contaminated by bacteria or not, while fecal Coliform bacteria are indicator bacteria polluting pathogenic bacteria originating from human feces and warm-blooded animals (mammals) . The water inspection method in this study uses the MPN (Most Probable Number) method which consists of 3 tests, namely, the presumption test, the affirmation test, and the reinforcement test. The results showed that of 15 drinking water samples 8 samples were tested positive for Coliform bacteria with the highest total bacterial value of sample number 1, 15 (210/100 ml), while 7 other samples were negative. From 8 positive Coliform samples only 1 sample was stated to be negative fecal Coliform bacteria and 7 other samples were positive for Coliform fecal bacteria with the highest total bacterial value of sample number 1 (210/100 ml).

scholarly journals Bacteria Identification In Traditional Sauce (Sambal) At The Restaurants Nearby The Traditional Markei Of Gorontalo City

2021 ◽  
Vol 2 (1) ◽  
pp. 11-18
Author(s):  
Ana Pepiana ◽  
Laksmyn Kadir ◽  
Agusrianto Yusuf
Keyword(s):  
Fecal Coliform ◽  
Sampling Technique ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
Fecal Coliform Bacteria ◽  
Negative Results ◽  
Escherchia Coli ◽  
Traditional Market ◽  
Positive Results

The unsafe food will cause the health problems in the community. Sambal which is known as the traditional sauce is a sauce made from chilies that are crushed until the water content comes out, giving it a spicy taste. Furthermore, Sambal is processed in a simple way so that it can leac to the contamination of microorganisms such as Coliform bacteria contamination. This study aims to determine the presence of Coliform bacteria contamination in Sambal at the supermarket nearby the traditional market in Gorontalo City. This research is a descriptive study using a qualitative approach. It examines the existence of Coliform bacteria using the Most Probable Number (MPN) method. The population in this study were 14 samples with the sampling technique by accidental sampling. Coliform bacteria examination results show that Sambal sample is found with positive results contaminated with Coliform bacteria, namely 11 samples with a percentage of 78.6% and negative results of 3 samples with a percentage of 21.4%. Based on the results, it can be concluded that a positive result was found consisting of 1 sample of coliform fekal bacteria, in this case the bakteri Escherchia coli. bacteria, and 10 samples from the non-fecal coliform bacteria.

scholarly journals Uji Bakteriologis pada Minuman Air Tebu yang Dijual di Pinggiran Jalan Khatib Sulaiman Kota Padang

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Dewi Oktavia Djasmi ◽  
Roslaili Rasyid ◽  
Eliza Anas
Keyword(s):  
Sugar Cane ◽  
Pathogenic Bacteria ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Index Method ◽  
Probable Number ◽  
E Coli ◽  
Cane Juice ◽  
Sugar Cane Juice ◽  
Mpn Test

Abstrak Higinitas penjual dan sanitasi lingkungan yang kurang bersih memungkinkan minuman tebu terkontaminasi oleh bakteri patogen. Lokasi penjualan minuman tebu pinggiran jalan yang terbanyak terletak di jalan Khatib Sulaiman kota Padang. Tujuan penelitian ini adalah menentukan kualitas minuman tebu apakah sesuai dengan persyaratanmikrobiologi.  Penelitian ini bersifat deskriptif dengan menggunakan metode indeks Most Probable Number (MPN) di bagian Mikrobiologi Fakultas Kedokteran Universitas Andalas Padang. Sampel pada penelitian ini adalah seluruh penjual minuman tebu yang ada, yaitu sepuluh minuman tebu yang berasal dari lima pedagang. Sepuluh sampel terdiridari lima minuman tebu yang dicampur es dan lima minuman tebu yang tidak dicampur es. Pemeriksaan MPN yang telah dilakukan terdiri dari dua tes, yaitu: tes presumtif dan tes konfirmatif. Hasilnya adalah seluruh sampel positif mengandung bakteri Coliform dan E. coli. Kesimpulan dari penelitian ini adalah air tebu yang dijual di pinggiran jalan Khatib Sulaiman Padang belum memenuhi standar kelayakan konsumsi secara bakteriologis dan terdapat perbedaan indeks MPN antara minuman tebu yang dicampur es dengan yang tidak dicampur es.Kata kunci: tes MPN, air tebu, hygiene Abstract Pathogenic bacteria can contaminate the sugar cane juice by the less hygiene and environmental sanitation of the sellers. There are a lot of people selling sugar cane juice in Padang, mostly in Khatib Sulaiman. The objective of this study was to determine the quality of the sugar cane juice accordance to microbiological requirements. This descriptive study was using the most probable number (MPN) index method and conducted in Microbiology Laboratory of Medical Faculty, Andalas University Padang.The sample in this study is all of sellers, which is ten cane juices fromfive sellers. Ten samples consists of five sugar cane water with ice and sugar cane water without ice MPN test was done by two tests, the presumptive test and the confirmative test. The result showed that all samples contained coliform bacteria and E.coli. The conclusion of this study is sugarca ne juice which is sold on roadside of Khatib Sulaiman is not worthy to microbiological standards for consumption and there is a difference between the MPN index sugar cane water with ice and sugar cane without ice.Keywords: MPN test, cane juice, hygiene

scholarly journals NILAI MPN (Most Probable Number) DAN DETEKSI BAKTERI Esherichia PADA MINUMAN ES JERUK PERAS di KOTA PONTIANAK

2019 ◽  
Vol 8 (3) ◽  
Author(s):  
Ratna Ratna ◽  
Rahmawati Rahmawati ◽  
Mukarlina Mukarlina
Keyword(s):  
Random Sampling ◽  
Pathogenic Bacteria ◽  
Sampling Method ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
Normal Limits ◽  
Index Value ◽  
Mpn Test ◽  
Test Result

Iced squeezed orange drinks is one one of processed drinks that made from orange. Good drinking water should not contain pathogenic bacteria or organisms that may harm human health. Coliform is one ofbacteria that may cause disease in humans and being used as indicator bacteria of pathogenic bacteria. This research was aimed to know the MPN value number of coliform bacteria and to identify the bacterial presence of genus Escherichia in iced squeezed orange drinks sold in Pontianak City. The sampling used random sampling method. MPN method was used in order to know the number of coliform in iced squeezed orange drinks in Pontianak City. MPN test result obtained the lowest index value of MPN/mL was 0,14/mL in sample in subdistrict Pontianak City and the highest index value in four subdistricts in Pontianak City was MPN/mL >24,00/mL. It was showed that five samples exceed normal limits in accordance with SNI standards SNI 01-3839-1995 based on Permenkes No 492/MENKES/per/IV/2010. Identification resultshowed the bacterial genus of coliform obtained was member of genus Escherichia.

scholarly journals Uji Kualitas Mikrobiologis Minuman Teh Poci yang Dijual Pedagang Kaki Lima di Pasar Raya Padang

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Genta Pradana ◽  
Roslaili Rasyid ◽  
Edison Edison
Keyword(s):  
Water Contamination ◽  
Microbiological Quality ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
Significant Movement ◽  
Traditional Market ◽  
The Subject ◽  
Two Phases

Abstrak Minuman Teh Poci merupakan produk minuman yang berkembang pesat dan banyak dijual oleh pedagang kaki lima di Padang terutama di Pasar Raya kota Padang. Penjualan Teh Poci oleh pedagang kaki lima di Pasar Raya tidak mengikuti sistem franchise yang mempunyai standar kebersihan penyajian menyebabkan proses pembuatan Teh Pociyang  dijual di Pasar Raya tidak terjamin kebersihan penyajianya. Tujuan penelitian ini adalah menguji kualitas mikrobiologis  minuman Teh Poci yang dijual pedagang kaki lima di Pasar Raya Padang. Minuman yang diperiksa adalah minuman Teh Poci yang dijual di sepanjang jalan Pasar Raya dengan mengambil 13 sampel. Penelitian dilakukan dari bulan November 2011- September 2012 dengan menggunakan metode Most Probable Number (MPN) yang terdiri dari tes penduga dan tes konfirmasi. Dari 13 sampel minuman yang diperiksa seluruh sampel tersebutmengandung bakteri Coliform. Faktor-faktor yang dapat mempengaruhi adalah kebersihan penjual minuman dalam menutup kemasan minuman, kurangnya higiene perseorangan, lokasi penjualan di tengah pasar, es batu yang tidak bersih, dan alat-alat yang digunakan. Kesimpulan penelitian ini ialah minuman Teh Poci yang dijual di Pasar Raya Padang tidak memenuhi standar kualitas mikrobiologis air minum yang sudah ditetapkan pada Peraturan Menteri Kesehatan tahun No. 492 tahun 2010.Kata kunci: pedagang kaki lima, teh Poci, higieneAbstract Teh Poci has become popular nowadays. There is a significant movement of Teh Poci street sellers in Padang especially in Pasar Raya Padang. Teh Poci street sellers in Pasar Raya do not buy the franchise system which has the appropriate standard for food serving. The Teh Poci stands are also located in the middle of traditional market which cause the serving to the society do not fulfill the sanitary standard.One of the indicators of water contamination is by measuring the amount of the coliform bacteria presents in water.  The objective of this study was to do themicrobiological quality test on Teh Poci in Pasar Raya Padang. The subject of this test was thirteen Teh Poci sold around Pasar Raya Street. The research occured from November 2011 until September 2012 using the Most Probable Number method, consists of two phases presumtive and confirmative.The result of the test showed that all thirteensamples were positive which means all the samples did not fulfill the microbiological quality of drinks published by the Indonesian Health Ministry.Keywords:  street sellers, Teh Poci, higiene

scholarly journals Microbial Contamination Analysis of Mobile Phones from certain Users of Chattogram, Bangladesh

2021 ◽  
Vol 14 (4) ◽  
pp. 1550-1556
Author(s):  
Mohammad Zobaidul Alam
Keyword(s):  
Mobile Phone ◽  
Mobile Phones ◽  
Pathogenic Bacteria ◽  
Antibiotic Sensitivity ◽  
Coliform Bacteria ◽  
Personal Hygiene ◽  
Diffusion Method ◽  
Most Probable Number ◽  
Probable Number ◽  
Hospital Patients

Mobile phone is an essential part of everyday life in modern days. Mobile phones act as vehicles for transmitting pathogenic bacteria due to lack of awareness and widespread use. This study aimed to investigate the bacterial contamination of mobile phones of different categories people at Chattogram city, Bangladesh. During the present study, 40 swab samples were collected from the mobile phones of students, businessmen, fishermen, and hospital patients for the isolation, identification of mobile phone associated bacteria, and their antibiogram. In our study, total viable count (TVC) was performed by the pour plate method and total coliform count (TCC) by the most probable number (MPN) method. Besides these, five selective media were used to isolate pathogenic bacteria from mobile phones and then identified. Antibiotic sensitivity assay was performed by disc diffusion method with 10 different antibiotics. Mobile phones of hospital patients (20165 cfu/ml) and students (1578 cfu/ml) showed the highest and lowest TVC respectively. Coliform bacteria were detected from the mobile phones of 100% hospital patients, 90% from both businessmen, and fishermen but only 30% from students. Klebsiella pneumoniae and Pseudomonas aeruginosa were found the most prevalent bacteria but Staphylococcus aureus, Staphylococcus epidermidis, Micrococcus sp., Bacillus sp., E. coli, Salmonella sp., Citrobacter sp., Serratia sp., Proteus sp., and Enterobacter sp. were also detected. Almost all the isolates were highly resistant to ampicillin, amoxicillin, rifampin, erythromycin and sensitive to ciprofloxacin, gentamicin, azithromycin, and tetracycline. Our findings ensure that mobile phones act as an important source of pathogenic organisms for humans and can serve as a vehicle for cross-transmission of microbiota. So, washing hands before and after handling food and also personal hygiene is very important.

scholarly journals THE TEST OF BACTERIAL CONTAMINATION ON MEATBALL PRODUCTS IN THE TRADITIONAL MARKET OF MARISA, POHUWATO

2021 ◽  
Vol 2 (1) ◽  
pp. 44-50
Author(s):  
Asnita Kamumu ◽  
Rita Amini Warastuti ◽  
Agusrianto Yusuf
Keyword(s):  
Bacterial Contamination ◽  
Human Growth ◽  
Microbial Pathogens ◽  
Coliform Bacteria ◽  
Plate Count ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Total Plate Count ◽  
Traditional Market

ABSTRACT Meatball is one of Indonesia's typical meat-based products. It is usually served at a high temperature, moreover, it contains high nutrient due to the high animal nutrient that is necessary for human growth. The current study aims to determine the test of bacterial contamination on meatball that are sold in the traditional market of Marisa, district of Pohuwato. Five meatball samples were taken from five different vendors, and the sample testing was done in the laboratory of the faculty of science, technology, and health on July 13th, 2020. The test included the total plate count microbial pathogens contamination and E. coli bacteria in the meatballs. The data were analyzed descriptively and presented in a tabular to illustrate the total plate count of pathogen contamination and E. coli bacteria in the meatballs. According to SNI 01-3818-1995, the maximum total plate count (ALT) of colonies/g is 0.1x102, and for the E. coli bacteria, the AMP/g is <3.0. As the results. It shows that the total microbes on the meatballs in the traditional markets of Marisa is ranged between 4.5x104 colonies/g to 2.3x105 colonies/g. It also shows that the meatballs still meet the requirement for the counts of microbial colonies, while the most probable number (AMP) of coliform bacteria is still high

scholarly journals Karakteristik Genus Bakteri Pada Karkas Ayam Broiler Dari Swalayan di Kota Pontianak

2018 ◽  
Vol 7 (3) ◽  
Author(s):  
Prianti Rahmawati Diah Wulandari Rousdy
Keyword(s):  
Broiler Chicken ◽  
Pathogenic Bacteria ◽  
Chicken Meat ◽  
Bacterial Isolates ◽  
Bacterial Genus ◽  
Chicken Carcass

The availability  of nutrients in chicken carcasses can cause chicken meat to be an excellent medium for the growth of pathogenic and non-pathogenic bacteria. This study aims to determine the characteristics of the bacterial genus in broiler chicken carcasses from supermarkets in Pontianak City. Based on the results of the study found 23 bacterial isolates in broiler chicken carcass samples from supermarkets in Pontianak City, which included members of the Aeromonas, Acetobacter, Alcaligenes, Amphibacillus, Bacillus, Brevibacterium, Camphylobacter, Carnobacterium, Erwinia,  Erysipelothrik, Eubacterium, Hafnia, Kluyvera, Klebsiella, Kurthia, Lactobacillus, Listeria, Proteus, Pseudomonas, Shigella, Sporolactobacillus, Serratia, and  Yersinia.

scholarly journals Escherichia coli O157:H7 in Drin Prevalence of Escherichia coli O157:H7 in Drink from Different Food Premises in Kota Samarahan Sarawak

2019 ◽  
Vol 2 (2) ◽  
pp. a13-19
Author(s):  
ELEXSON NILLIAN ◽  
AMIZA NUR ◽  
DIYANA NUR ◽  
AMIRAH ZAKIRAH ◽  
GRACE BEBEY
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Plain Water ◽  
Escherichia Coli O157 ◽  
Probable Number ◽  
E Coli ◽  
Preventive Actions ◽  
Stx1 Gene

Contamination of drinks with E. coli O157:H7 served in food premises such as restaurants can cause haemorrhagic colitis and haemolytic uremic syndrome to humans. The presence or absence of faecal pathogen was demonstrated using coliform group as indicator microorganisms. Therefore, this study was conducted to detect the presence of E. coli O157:H7 in drinking water from food restaurant premise in Kota Samarahan and Kuching to ensure safe and potable drinking water is served to the consumer. A total of thirty (n=30) drink samples including six types of each of the samples are cold plain water, iced tea, iced milo, syrup and iced milk tea. Most Probable Number (MPN) procedure was used in this study to enumerate the MPN values of coliform bacteria in each drink collected. A total of 53.33% (16/30) of the drink samples showed positive E. coli detection. Then, the PCR assay showed 6.25% (one out of 16 isolates) samples were positive and carried stx1 gene produced by E. coli O157:H7 in iced milo sample types. This study showed the drinks collected from food premises was contaminated with faecal contamination, which was not safe to drink by the consumer. Therefore, preventive actions should be taken to prevent foodborne illness outbreak in future

scholarly journals ANALISA MIKROBIOLOGI CEMARAN BAKTERI COLIFORM DAN SALMONELLA SP PADA TAHU DI KECAMATAN DELTA PAWAN

2019 ◽  
Vol 6 (1) ◽  
pp. 61
Author(s):  
Nenengsih Verawati ◽  
Nur Aida ◽  
Ridha Aufa
Keyword(s):  
Coliform Bacteria ◽  
Plate Count ◽  
Most Probable Number ◽  
Food Ingredient ◽  
Probable Number ◽  
West Kalimantan ◽  
Safety Standards ◽  
Confirmatory Tests ◽  
Food Safety Standards ◽  
Positive Results

Tofu is a high-protein soy-based food ingredient that is widely consumed in Indonesia. Tofu producers in Delta Pawan Subdistrict are dominated by small and medium-sized entrepreneurs whose hygiene and sanitation aspects in production activities are very under-taken. This study aims to determine the presence of Coliform and Salmonella Sp bacteria on tofu produced in Delta Pawan sub-district, Ketapang, West Kalimantan and compare with SNI01-3142-1998. The method used to detect the presence of Coliform bacteria in this study uses the Most Probable Number (MPN) method, which consists of estimator and confirmatory tests using Lactosa Broth (LB) media for the Brilliant Green Lactose Broth (BGLB) assay test for confirmation . Whereas to detect the amount of Salmonella Sp using the method of Total Plate Count (TPC) with selective media Salmonella Shigella Agar (SSA). The results of Coliform analysis in both industries found Coliform MPN values ​​of more than 2400 AMP / g samples, which indicated that they did not meet the standards set by SNI. While the results of the analysis of Salmonella Sp obtained positive results, so the two industries did not meet food safety standards.

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