Pharmaceutico-analytical profile of  Mrityunjay Rasa and evaluation of its Antibacterial activity

Background: Mrityunjay Rasa is the one of the Kharaliya Kalpana explained in Yogaratnakara in Jwara chikitsa and indicated in all types of fever. Any pathology can leads to diseases which are easily seen by fever as a clinical symptom. Bacterial infections through the skin or by any system defiantly reflect through fever and as told in classics Mrityunjay Rasa is having the capacity to eradicate all types of Jwara. Aim & Objective: The study is planned to evaluate the antibacterial activity of Mrityunjay Rasa. Material and methods: Mrityunjay rasa was prepared according to Yogratnakar at the Department of Rasashastra & Bhaishajya Kalpana. The formulation was tested for organoleptic characters, physicochemical parameters and microbial specification tests. Observation and results: Standard Mrityunjay rasa can be prepared from three Bhawana of Ardraka swarasa each for an average of 11.55 hrs. Prepared Mrityunjay rasa was Reddish in color with a specific smell and 145.57% average yield. Analytical standards for Mrityunjay rasa such as loss on drying at 105oC, total ash, acid insoluble ash, alcohol soluble extractives, water-soluble extractive, pH, Hardness, Disintegration time and particle size were 2.10%, 15.87%, 2.43%, 2.29%, 20.43 %, 8.7 (10% aqueous solution), 3, 30 minutes 20 sec and 95.41 respectively. Mrityunjay Rasa has shown a better zone of inhibition against Staphylococcus aureus. Conclusion: Analytical parameters obtained through the present study can be considered as a reference standard for Mrityunjay rasa.

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Synthesis and Antibacterial Activity of 3-(Substituted)-2-(4-oxo-2-phenylquinazolin-3(4H)-ylamino)quinazolin-4(3H)-one

Scientifica ◽

10.1155/2016/1249201 ◽

2016 ◽

Vol 2016 ◽

pp. 1-5 ◽

Cited By ~ 2

Author(s):

Ramgopal Appani ◽

Baburao Bhukya ◽

Kiran Gangarapu

Keyword(s):

Antibacterial Activity ◽

Bacillus Subtilis ◽

Primary Amines ◽

Reference Standard ◽

Proteus Vulgaris ◽

Zone Of Inhibition ◽

Active Compounds ◽

Multistep Synthesis

A series of novel 3-(substituted)-2-(substituted quinazolinylamino)quinazolin-4(3H)-ones were synthesized by the reaction of 3-(substituted)-2-hydrazino-quinazoline-4(3H)-ones with 2-phenyl-3,1-benzoxazin-4-one. The starting materials 3-(substituted)-2-hydrazino-quinazolin-4(3H)-ones were synthesized from various primary amines by a multistep synthesis. All the title compounds were tested for their antibacterial activity using ciprofloxacin as reference standard. Compounds 3-(4-fluorophenyl)-2-(4-oxo-2-phenylquinazolin-3(4H)-ylamino)quinazolin-4(3H)-one (9a) and 3-(4-chlorophenyl)-2-(4-oxo-2-phenylquinazolin-3(4H)-ylamino)quinazolin-4(3H)-one (9h) emerged as the most active compounds of the series. These compounds have shown most potent antibacterial activity against the tested organisms ofProteus vulgarisandBacillus subtilishaving zone of inhibition values of 1.1 cm and 1.4 cm for compound9a1.2 cm and 1.0 cm for compound9h, respectively.

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FORMULATION AND EVALUATION OF NOVEL ANTIBACTERIAL AND ANTI-INFLAMMATORY CREAM CONTAINING MUNTINGIA CALABURA LEAVES EXTRACT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i12.21963 ◽

2017 ◽

Vol 10 (12) ◽

pp. 376

Author(s):

Mahendran Sekar ◽

Nur Syahira Abdul Jalil

Keyword(s):

Antibacterial Activity ◽

Bacterial Infections ◽

Stable State ◽

Inflammatory Activity ◽

Diffusion Method ◽

Zone Of Inhibition ◽

Gram Positive Bacteria ◽

Maximum Inhibition ◽

Anti Inflammatory ◽

Muntingia Calabura

Objective: The main objective of the present study was to formulate antibacterial and anti-inflammatory cream using Muntingia calabura leaves extract.Methods: 500 g of matured leaves of M. calabura were extracted with 70% methanol using maceration method for a week. The antibacterial activity of the extract and formulated cream in different concentration was investigated against two Gram-positive bacteria (Staphylococcus aureus and Bacillus cereus) and two Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) through disc diffusion method. The anti-inflammatory activity of extract and formulated cream was studied using inhibition of albumin denaturation technique.Results: The antibacterial activity of the methanolic extract was studied in different concentrations (5, 10, and 25 mg/ml) and the formulated cream was studied in the concentration of (10, 25, 50, and 100 mg/ml). The extract showed significant antibacterial activity with all the tested pathogens with a zone of inhibition ranges from 3.33 ± 0.88-14.60 ± 0.80 mm. As for the formulated cream, the zone of inhibition for F1 ranged from 0.67 ± 0.67 mm to 14.80 ± 1.17 mm. F2 presented zone of inhibition at 3.40 ± 1.64 mm to 15.00 ± 1.18 mm. The anti-inflammatory activity showed maximum inhibition of 63.93 ± 5.90, 41.83 ± 5.64, and 27.20 ± 2.72 was observed at 1000 μg/ml for the extract, F2 and F1, respectively. The cream was found to be in stable state after 2 months.Conclusion: The results showed that the formulated cream can be used for bacterial infections and to reduce inflammation. We recommend this formulation need to be further developed into commercial standards.

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Isolation of antibiotics producing bacteria from marine soil and comparative analysis of same with commercially available drugs

GSC Biological and Pharmaceutical Sciences ◽

10.30574/gscbps.2021.16.1.0167 ◽

2021 ◽

Vol 16 (1) ◽

pp. 070-076

Author(s):

Chinyelu Nkiru Umeaku ◽

Chisom Faith Ohagwam ◽

Chiamaka Ijeoma Chris-Umeaku

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Comparative Analysis ◽

Bacterial Infections ◽

Streptomyces Griseus ◽

Zone Of Inhibition ◽

Preliminary Screening ◽

Port Harcourt ◽

Marine Soil

The isolation of antibiotic producing bacteria from marine soil and comparative analysis of same with ciprofloxacin and amoxicillin against staphylococcus aureus and Escherichia coli was carried out in a Microbiology Laboratory of Chukwuemeka Odumegwu Ojukwu University, Uli. This was done to isolate antibiotic producing bacteria and compare same with existing commercially available antibiotics with a view to using marine soil in the treatment of common bacterial infections. Soil samples were collected from Bonny Island Sea, Port Harcourt. One gram of mixed soil sample was serially diluted and spread-plated on nutrient agar plates. The representative isolates obtained were sub-cultured to get a pure culture. Morphological, biochemical, physiological characteristics of the bacteria were analyzed. Agar well diffusion was carried out. One isolate had a substantial antibacterial activity with 3.5mm zone of inhibition against two test bacteria used in the preliminary screening. The isolate was marked as Streptomyces (STR I) and was identified as Streptomyces griseus while other isolates did not show any antibacterial activity. Ciprofloxacin showed the highest antibacterial activity to both Staphylococcus aureus and Escherichia coli of 3.7mm and 4.0mm respectively while Amoxicillin showed antibacterial activity of 3.5mm and 2.7mm respectively. This reveals that antibiotic producing bacteria from marine soil are also effective in antimicrobial activity and could be used for antimicrobial chemotherapy.

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Formulation and Evaluation of Orally Disintegrating Tablets of Lamotrigine

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2015.8.2.11 ◽

2015 ◽

Vol 8 (2) ◽

pp. 2881-2888

Author(s):

Sudarshan Singh ◽

S S Shyale ◽

P Karade

Keyword(s):

Drug Release ◽

Water Soluble ◽

In Vitro Dissolution ◽

Disintegration Time ◽

Orally Disintegrating Tablet ◽

Drug Content ◽

Weight Variation ◽

Wetting Time ◽

Croscarmellose Sodium

The aim of this study was to design orally disintegrating tablet (ODT) of Lamotrigine. It is an Antiepileptic drug which is widely used in epilepsy. It is also used in simple and complex partial seizures and secondary generalized tonic-clonic seizures. It is poorly water soluble drug (0.46 mg/ml). Thus, an attempt was made to enhance the water solubility by complexation with β-cyclodextrin (1:1 molar ratios). The orally disintegrating tablet of lamotrigine was prepared by direct compression method using different concentration of superdisintegrants such as Sodium starch glycollate, croscarmellose sodium by sublimating agent such as camphor. The formulations were evaluated for weight variation, hardness, friability, drug content, wetting time, in vitro disintegration time and in vitro dissolution studies. The prepared tablets were characterized by Fourier transform infrared spectroscopy and differential scanning calorimetry. The disintegration time for the complexed tablets prepared by different concentration of superdisintegrants was found to be in range of 32.54 ± 0.50 to 55.12 ± 0.57 sec and wetting time of the formulations was found to be in range of 28.47 ± 0.67 to 52.19 ± 0.72 sec. All the formulation showed almost 100 percent of drug release within 15 min. Among all the formulation F6 and F7 prepared with 18% croscarmellose sodium and camphor shows faster drug release, respectively 10 min, F6 gives good result for disintegration time, drug release, wetting time and friability. Further formulations were subjected to stability testing for 30 days at temperature of 40 ± 5 ºC/75 ± 5 %RH. Tablets showed no appreciable changes with respect to physical appearance, drug content, disintegration time and dissolution profiles. Results were statistically analyzed by one-way ANOVA at a p < 0.05. It was found that, the data at any point of time are significant at p < 0.05.

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Fluoroquinolone-3-carboxamide Amino Acid Conjugates: Synthesis, Antibacterial Properties And Molecular Modeling Studies

Medicinal Chemistry ◽

10.2174/1573406415666190904143852 ◽

2020 ◽

Vol 17 (1) ◽

pp. 71-84

Author(s):

Riham M. Bokhtia ◽

Siva S. Panda ◽

Adel S. Girgis ◽

Hitesh H. Honkanadavar ◽

Tarek S. Ibrahim ◽

...

Keyword(s):

Experimental Data ◽

Antibacterial Activity ◽

Amino Acid ◽

Bacterial Infections ◽

Antibacterial Agents ◽

Antibacterial Properties ◽

Computational Studies ◽

Protecting Group ◽

Amino Acid Conjugates ◽

Molecular Modeling Studies

Background: Bacterial infections are considered as one of the major global health threats, so it is very essential to design and develop new antibacterial agents to overcome the drawbacks of existing antibacterial agents. Method: The aim of this work is to synthesize a series of new fluoroquinolone-3-carboxamide amino acid conjugates by molecular hybridization. We utilized benzotriazole chemistry to synthesize the desired hybrid conjugates. Result: All the conjugates were synthesized in good yields, characterized, evaluated for their antibacterial activity. The compounds were screened for their antibacterial activity using methods adapted from the Clinical and Laboratory Standards Institute. Synthesized conjugates were tested for activity against medically relevant pathogens; Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27856) Staphylococcus aureus (ATCC 25923) and Enterococcus faecalis (ATCC 19433). Conclusion: The observed antibacterial experimental data indicates the selectivity of our synthesized conjugates against E.Coli. The protecting group on amino acids decreases the antibacterial activity. The synthesized conjugates are non-toxic to the normal cell lines. The experimental data were supported by computational studies.

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The component composition of planted pine wood cultivated in the boreal zone

Nordic Pulp & Paper Research Journal ◽

10.1515/npprj-2020-0058 ◽

2020 ◽

Vol 35 (4) ◽

pp. 685-693

Author(s):

D. A. Danilov ◽

A. V. Griazkin ◽

V. A. Sokolova ◽

I. V. Bacherikov

Keyword(s):

Component Composition ◽

Water Soluble ◽

Boreal Zone ◽

Lower Content ◽

Average Yield ◽

Leningrad Oblast ◽

Pine Wood ◽

Natural Stands

AbstractThe present study analyzes the composition of pine wood cultivated on artificial plantations in the Leningrad Oblast (Region). Comparing to pine wood from natural stands, a smaller heartwood zone along the height of the pine trunk and a lower content of resinous substances are noted. The content of cellulose and lignin in the heartwood and sapwood of pine is distributed differently along the trunk. The distribution of pentosans and water-soluble substances in the heartwood and sapwood along the trunk is associated with the lignin complex. The component composition of the studied pine wood from planted stands is quite uniform in its indicators if compared to the wood of older natural stands. Sulphate pulping of planted pine wood showed a higher yield of technical cellulose than the average yield from wood obtained at natural stands of the studied region.

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Synthesis, Characterization and Bactericidal Activity of a 4-Ammoniumbuthylstyrene-Based Random Copolymer

Polymers ◽

10.3390/polym13071140 ◽

2021 ◽

Vol 13 (7) ◽

pp. 1140

Author(s):

Silvana Alfei ◽

Gabriella Piatti ◽

Debora Caviglia ◽

Anna Maria Schito

Keyword(s):

Antibacterial Activity ◽

Bactericidal Activity ◽

Antimicrobial Agents ◽

Water Solubility ◽

Physicochemical Characterization ◽

Random Copolymer ◽

Water Soluble ◽

Cationic Polymers ◽

Severe Infections

The growing resistance of bacteria to current chemotherapy is a global concern that urgently requires new and effective antimicrobial agents, aimed at curing untreatable infection, reducing unacceptable healthcare costs and human mortality. Cationic polymers, that mimic antimicrobial cationic peptides, represent promising broad-spectrum agents, being less susceptible to develop resistance than low molecular weight antibiotics. We, thus, designed, and herein report, the synthesis and physicochemical characterization of a water-soluble cationic copolymer (P5), obtained by copolymerizing the laboratory-made monomer 4-ammoniumbuthylstyrene hydrochloride with di-methyl-acrylamide as uncharged diluent. The antibacterial activity of P5 was assessed against several multi-drug-resistant clinical isolates of both Gram-positive and Gram-negative species. Except for strains characterized by modifications of the membrane charge, most of the tested isolates were sensible to the new molecule. P5 showed remarkable antibacterial activity against several isolates of genera Enterococcus, Staphylococcus, Pseudomonas, Klebsiella, and against Escherichia coli, Acinetobacter baumannii and Stenotrophomonas maltophilia, displaying a minimum MIC value of 3.15 µM. In time-killing and turbidimetric studies, P5 displayed a rapid non-lytic bactericidal activity. Due to its water-solubility and wide bactericidal spectrum, P5 could represent a promising novel agent capable of overcoming severe infections sustained by bacteria resistant the presently available antibiotics.

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Electrospun PU/MgO/Ag Nanofibers for Antibacterial Activity and Flame Retardency

Clothing and Textiles Research Journal ◽

10.1177/0887302x211009479 ◽

2021 ◽

pp. 0887302X2110094

Author(s):

V. Mamtha ◽

H. N. Narasimha Murthy ◽

V. Pujith Raj ◽

Prashantha Tejas ◽

C. S. Puneet ◽

...

Keyword(s):

Antibacterial Activity ◽

Melting Point ◽

Protective Clothing ◽

Electrospun Nanofibers ◽

Synthesis And Characterization ◽

Zone Of Inhibition ◽

Solution Combustion ◽

E Coli ◽

Afterglow Time

Antibacterial activity and fire retardation are equally desired for protective clothing. For achieving this, AgNP and MgO are independently researched as nanofillers in Polyurethane based electrospun nanofibers and their synergistic effect is scarcely addressed. This article reports synthesis and characterization of MgO of 70.01 nm and AgNP of 51 to 76 nm by solution combustion and hydrothermal routes respectively and their incorporation in electrospinning of Polyurethane. Flow rate 1 ml/hr, applied voltage 13 kV, tip to collector distance 15 cm were adopted for the electrospinning. Nanofibers of 65 nm were obtained for PU/MgO (3 wt. %) and 106 nm for PU/MgO (3 wt. %)/Ag (1 wt. %). Addition of MgO increased the melting point, after flame time and afterglow time. Incorporation of AgNP improved antibacterial activity. PU/MgO/Ag (2 wt. %) exhibited zone of inhibition of 2.1 cm and 3 cm against E. Coli and S. Aureus, respectively.

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A Water-Soluble Copper-Immobilized Covalent Organic Framework Functioning as an “OFF–ON” Fluorescent Sensor for Amino Acids

Materials Advances ◽

10.1039/d1ma00234a ◽

2021 ◽

Author(s):

Lamiaa Reda Ahmed ◽

Ahmed F. M. EL-Mahdy ◽

Cheng-Tang Pan ◽

Shiao-Wei Kuo

Keyword(s):

Amino Acids ◽

Fluorescent Sensor ◽

Water Soluble ◽

One Pot ◽

Covalent Organic Framework ◽

The One ◽

Organic Framework

In this paper, we describe the construction of a new fluorescent hydroxyl- and hydrazone-based covalent organic framework (TFPB-DHTH COF) through the one-pot polycondensation of 1,3,5-tris(4-formylphenyl)benzene (TFPB) and 2,5-dihydroxyterephthalohydrazide (DHTH) under...

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A New Supported Manganese-Based Coordination Complex as a Nano-Catalyst for the Synthesis of Indazolophthalazinetriones and Investigation of Its Antibacterial Activity

Chemistry ◽

10.3390/chemistry3030056 ◽

2021 ◽

Vol 3 (3) ◽

pp. 783-799

Author(s):

Maryam Ariannezhad ◽

Davood Habibi ◽

Somayyeh Heydari ◽

Vahideh Khorramabadi

Keyword(s):

Antibacterial Activity ◽

Simple Procedure ◽

Condensation Reaction ◽

Antibacterial Properties ◽

Coordination Complex ◽

Bacterial Strains ◽

One Pot ◽

Nano Catalyst ◽

Ft Ir ◽

The One

A new magnetic supported manganese-based coordination complex (Fe3O4@SiO2@CPTMS@MBOL@ Mn) was prepared in consecutive stages and characterized via various techniques (VSM, SEM, TEM, XRD, FT-IR, EDX, TG-DTA, and ICP). To evaluate its application, it was used for synthesis of divers Indazolophthalazinetriones in a simple procedure via the one-pot three-component condensation reaction of aldehydes, dimedone, and phthalhydrazide in ethanol under reflux conditions. The Mn catalyst can be recycled without any noticeable loss in catalytic activity. Additionally, the antibacterial properties of the nano-catalyst were studied against some bacterial strains.

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