Quantitative Determination of Aloin in Aloe vera and Its Antioxidant Activity
Abstract-Aloe verais a popular medicinal plant used widely by the cosmetic, pharmaceutical and food industries. The Aloe veragel, which is used mostly for its positive effects on human health, contains over 75 different bioactive compounds, includingaloin.AsensitiveandreliabledensitometricHighPerformanceThinLayerChromatography method has been developed for the quantification of aloin, an anthraquinone present in Aloe vera leaves. Chromatographic analysis was performed using methanol extract of leaves of Aloe verausing solvent systemethyl acetate: Methanol: water (100:13.5:10). Detection and quantification of aloin was done by densitometric scanning at 350nm. The results of linearity range and correlation coefficient show that there was a good correlation between peak area and corresponding concentration of aloin. The method developed here in can be implemented in the analysis and routine quality control of herbal materials. Antioxidant activity of Aloe verawas also done by using DPPH method and found that Aloe verais also a natural antioxidant.