Effect of IMT-03, an Herbal Formulation in Cyclophosphamide-induced Immunosuppression in Mice

2021 ◽  
Vol 7 (1) ◽  
pp. 14-17
Author(s):  
Tapas Kumar Sur ◽  
Avijit Hazra
Keyword(s):  
Signaling Pathways ◽  
Radical Scavenging ◽  
Herbal Medicines ◽  
Food Habit ◽  
Serious Adverse Events ◽  
Lps Challenge ◽  
Cytokines And Chemokines ◽  
Herbal Formulation ◽  
The Individual

Abstract: Immumomodulatory actions of natural ingredients have been recognized from the time of early civilization. Though herbal medicines are equally used to treat deadly diseases, but till date there are lacks of evidences. In the present work, an herbal preparation IMT-03 was used to find out its immunostimulatory efficacy against chemotherapeutics. Although, most of the individual ingredients present in IMT-03 have been reported for their protective roles against chemotherapeutics, but there is no report in combination. Cyclophosphamide (300 mg/kg, intraperitoneally) induces immunosuppression through myelosuppression. Pretreatment with of IMT-03 at oral the dose of 100 mg/kg and 200 mg/kg in Cyclophosphamide induced immunosuppressive mice showed dose dependently and significantly (p<0.001) improvement in total WBC and absolute neutrophil counts. It also protect the  general health of animals from chemotherapeutic induced serious adverse events like signs of sickness, lethargy, immobility, reducing food habit, infections in nostrils and pinna etc. Moreover, IMT-03 has the abilities to counter the macrophages surge during the LPS challenge. The test drug also showed safe up to the oral dose of 2 g/kg. In vitro studies revealed IMT-03 has polyphenols and also radical scavenging actions. There are several evidences that polyphenols can able to modulate cytokines and chemokines signaling pathways in immune cells. Therefore, it is assumed that polyphenols present in IMT-03 either modulate the inflammatory signaling pathways or protect from oxidative stress related DNA damage in myeloid tissues.  

scholarly journals Determination of in Vivo Biological Activities of Dodonaea Viscosa Flowers Against CCL4 Toxicity in Albino Mice With Bioactive Compound Detection

2021 ◽  
Author(s):  
Hina Gul ◽  
Muhammad Awais ◽  
Salina Saddick ◽  
Falak Sher Khan ◽  
Muhammad Gulfraz ◽  
...  
Keyword(s):  
Protective Effect ◽  
Biological Activities ◽  
Hematological Parameters ◽  
Octadecenoic Acid ◽  
Radical Scavenging ◽  
Herbal Medicines ◽  
Bioactive Compound ◽  
Dodonaea Viscosa

Abstract Dodonaea viscosa L. Jacq. is an evergreen shrub and native to Asia, Africa and Australia. It has been used as traditional medicine in different countries. The foremost objective of the current study was to discover protective potential of D. Viscosa flowers Methanol (DVM) and Chloroform (DVC) extracts against CCL4 induced toxicity in mice. This study was intended to identify phytochemicals through HPLC, GCMS and FT-IR as well as in vitro antioxidant and in vitro antituberculosis activity. Our comprehensive findings indicate that Dodonaea viscosa is valuable and widespread herbal medicines through therapeutic potentials for curing various ailments. Dodonaea viscosa flowers are found to have protective effect against oxidative stress produced by CCL4 in liver, kidney and spleen. The level of hepatic enzymes (ALP, AST ALT and Direct bilirubin), hematological parameters (RBCs, WBCs and Platelets), total protein and liver antioxidant enzymes (SOD, GPx and CAT) were restored by the intake of DV extracts after decline in levels by CCL4. Histopathological results discovered the defensive effect of 300mg/kg of DVM extract against CCL4 induced damage, thus having improved protective effect as compared to DVC and control. As a result of analysis total flavonoids and total phenolics were also revealed. Phytochemical investigation by HPLC identified gallic acid, epicatechin, cumeric acid, flavonoids while Oleic acid (Octadecenoic acid) (C18H34O2), Stearic acid (C18H36O2), Ricinoleic acid (C18H34O3) and Cedrol (C15H26O) was estimated by GCMS. DVM extract exhibited resistance against in vitro Mycobacterium tuberculosis strains. This study proposed that protective effect of DV against oxidative damage induced in Liver, Kidney and Spleen can possibly be correlated to their antioxidant as well as free radical scavenging property.

IN-SILICO AND IN VITRO ASSESSMENT OF SYNTHESIZED DIAZENYLSULFONAMIDES AS APOPTOSIS INDUCERS AND RADICAL SCAVENGERS

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (06) ◽  
pp. 49-59
Author(s):  
Priyambada Kshiroda Nandini Sarangi ◽  
Jyotirmaya Sahoo ◽  
Chita Ranjan Sahoo ◽  
Sudhir Kumar Paidesetty ◽  
Guru Prasad Mohanta
Keyword(s):  
Cell Line ◽  
Cancer Cell ◽  
Radical Scavenging ◽  
Cancer Cell Line ◽  
Scavenging Effect ◽  
Ic50 Values ◽  
Radical Scavenging Effect ◽  
The Individual ◽  
Mcf 7

A series of eight quinoline-thiazole hybrid-bearing diazenylsulfonamides, 4a-4h, were synthesized and characterized by UV-Vis, FT/IR, 1H NMR and lC-MS. These compounds were formed when two prepared intermediate precursors of Schiff-base compounds, (E)-N-((2-chloroquinolin-3-yl)methylene)-4phenylthiazol-2-amine (3a) and (E)-N-((2-chloroquinolin-3-yl)methylene)-4-chlorophenylthiazol-2-amine (3b) were converted to the corresponding diazenyl compounds 4a-4h by treating and coupling with the individual diazonium salts of sulfa-drugs. The results of in vitro cytotoxic activity of the synthesized compounds in two cancer cell lines MCF 7 (human breast cancer cell line) and K562 (myelogenousleukemia cell line) have shown the IC50 values as given: 4b against MCF 7 19.52 and against K562 20.55µM; 4d against MCF 7 15.96 and against K562 13.05µM. Moreover, the compound 4-(((Z)-(2-chloroquinolin-3yl)(4-phenylthiazol-2-ylimino)methyl)diazenyl)benzenesulfonic acid (4d) induced maximum percentage of apoptosis. Furthermore, the in vitro antioxidant activity study revealed that among all the synthesized compounds, compound 4d has an excellent radical scavenging effect. Molecular docking was additionally performed to investigate the binding affinity of H-bonding interaction of synthesized compounds with a targeted enzyme and to compare it with the anticancer drugs, dasatinib, bosutinib and dacarbazine.

scholarly journals Network Pharmacology/Metabolomics-Based Validation of AMPK and PI3K/AKT Signaling Pathway as a Central Role of Shengqi Fuzheng Injection Regulation of Mitochondrial Dysfunction in Cancer-Related Fatigue

2021 ◽  
Vol 2021 ◽  
pp. 1-23
Author(s):  
Wei Guo ◽  
Shan Liu ◽  
Xinting Zheng ◽  
Zhiwei Xiao ◽  
Hanrui Chen ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Muscle Cell ◽  
Herbal Medicines ◽  
Enrichment Analysis ◽  
Akt Signaling ◽  
Network Pharmacology ◽  
Cancer Therapies ◽  
Cancer Related Fatigue ◽  
Associated Proteins

Chinese herbal medicines have multiple targets and properties, and their use in multidisciplinary cancer therapies has consequently received increasing attention. Here, we have investigated the possible active ingredients associated with cancer-related fatigue (CRF) in the Shengqi Fuzheng Injection (SFI). In vitro cell models were used to measure the regulation effects of SFI on CRF. Metabolomic analysis was used to identify the potential genes and pathways in C2C12 mouse myoblasts treated with SFI, and the interaction of compounds and CRF targets was predicted using network pharmacology and molecular docking analyses. The putative pathways were further verified using immuno-blotting assays. The results showed that SFI significantly inhibited muscle cell apoptosis and increased the mitochondrial membrane potential of muscle cells. The network pharmacology analysis results identified 36 candidate compounds, and 244 potential targets were yielded by SFI, and they shared 10 key targets associated with cancer-related fatigue. According to the enrichment analysis and experimental validation, SFI might ameliorate muscle cell mitochondrial function by activating AMPK and inhibiting the PI3K/Akt signaling pathways, and the expression changes of mitochondrial metabolic enzymes MnSOD and apoptosis-associated proteins Bax and Bcl-2 were also triggered. The functions and mechanisms of SFI in anticancer-related fatigue were found here to be at least partly due to the targeting of the AMPK and PI3K/Akt signaling pathways, and this has highlighted new potential applications for network pharmacology when researching Chinese Medicines.

Evaluation of the Antioxidant and Antidiabetic Potential of the Poly Herbal Formulation: Identification of Bioactive Factors

2020 ◽  
Vol 18 (2) ◽  
pp. 111-123
Author(s):  
V.V. Sathibabu Uddandrao ◽  
Parim Brahmanaidu ◽  
Saravanan Ganapathy
Keyword(s):  
Plant Extracts ◽  
Antioxidant Properties ◽  
In Vitro Models ◽  
Piper Nigrum ◽  
Individual Plant ◽  
Glucose Lowering ◽  
Methanolic Extracts ◽  
Herbal Formulation ◽  
The Individual

Background & Objectives: The present investigation is intended to prepare a Poly Herbal Formulation (PHF) with Piper nigrum (fruits), Terminalia paniculata (bark) and Bauhinia purpurea (bark) and assess their antioxidant and glucose-lowering effects utilizing in vitro models. Methods: The individual plant methanolic extracts and PHF are exposed to phytochemical examination and to distinguish the bioactive factors by GC-MS. We assessed the antioxidant properties of individual plant extracts and the PHF by using the DPPH scavenging method, H2O2 scavenging assay, TBARS assay and total antioxidant estimation. Likewise, the anti-diabetic activity was assessed by ɑ-amylase and α-glucosidase enzyme inhibition and glucose diffusion inhibitory techniques. Results: We found that PHF contains a high measure of total phenolics, total flavonoids and tannin compared to individual plant extracts. The GC-MS identified the bioactive components. We also found that PHF had significantly higher antioxidant and glucose-lowering effects than the individual plant concentrates. Conclusion: In conclusion, it could be reasoned that due to the nearness of antioxidant components, the PHF has good potential in the administration of hyperglycemia, diabetes and the related state of oxidative stress. This study shows that PHF is superior to individual plant extracts, supporting the conventional PHF concept.

scholarly journals Applications of the PhytomedicineEchinacea purpurea(Purple Coneflower) in Infectious Diseases

2012 ◽  
Vol 2012 ◽  
pp. 1-16 ◽  
Author(s):  
James B. Hudson
Keyword(s):  
Herbal Medicines ◽  
Echinacea Purpurea ◽  
Antimicrobial Activities ◽  
Immune Functions ◽  
Cytokines And Chemokines ◽  
Multiple Components ◽  
Purple Coneflower ◽  
Proinflammatory Responses ◽  
Potential Applications ◽  
The Individual

Extracts ofEchinacea purpurea(EP, purple coneflower) have been used traditionally in North America for the treatment of various types of infections and wounds, and they have become very popular herbal medicines globally. Recent studies have revealed that certain standardized preparations contain potent and selective antiviral and antimicrobial activities. In addition, they display multiple immune-modulatory activities, comprising stimulation of certain immune functions such as phagocytic activity of macrophages and suppression of the proinflammatory responses of epithelial cells to viruses and bacteria, which are manifested as alterations in secretion of various cytokines and chemokines. These immune modulations result from upregulation or downregulation of the relevant genes and their transcription factors. All these bioactivities can be demonstrated at noncytotoxic concentrations of extract and appear to be due to multiple components rather than the individual chemical compounds that characterizeEchinacea extracts. Potential applications of the bioactive extracts may go beyond their traditional uses.

In vitro antioxidant potential of Shemamruthaa (a herbal formulation) and its anticancer activity in the MCF-7 cell line

RSC Advances ◽  
2015 ◽  
Vol 5 (30) ◽  
pp. 23125-23133
Author(s):  
Nandhakumar Elumalai ◽  
Purushothaman Ayyakkannu ◽  
Shanthi Palanivelu ◽  
Sachdanandam Panchanadham
Keyword(s):  
Anticancer Activity ◽  
Cell Line ◽  
Ex Vivo ◽  
Radical Scavenging ◽  
Herbal Formulation ◽  
Hibiscus Rosa Sinensis ◽  
Line P ◽  
Mcf 7 ◽  
Free Radical Scavenging Activities

The current study investigates the free radical scavenging activities of methanolic and aqueous extracts of Shemamruthaa (Hibiscus rosa sinensis, Emblica officinalis and honey in definite ratio) and their anticancer activity in the breast cancer (MCF-7) cell line ex vivo.

scholarly journals Phytochemical profile and in vitro antioxidant activity of Emelia M (EMB), Mshikazi and Delosma H herbal medicines as demonstrated in THP-1 and Jurkat leukaemia cell lines

2021 ◽  
Vol 21 (4) ◽  
pp. 1924-37
Author(s):  
Joy Nkechinyere Adeniyi ◽  
Manimbulu Nlooto ◽  
Mlungisi Ngcobo ◽  
Roshila Moodley ◽  
Exnevia Gomo
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Herbal Medicines ◽  
Jurkat Cells ◽  
Thiobarbituric Acid Reactive Substance ◽  
Scavenging Activity ◽  
Phytochemical Profile ◽  
In Vitro Antioxidant Activity

Background: Three decoctions, namely Emelia M (EMB), Mshikazi and Delosma H are used by traditional health practitioners in KwaZulu-Natal, South Africa to treat and manage leukaemia and related conditionsObjectives: This study evaluated the in vitro antioxidant activity and phytochemical profile of the aqueous extracts of Emelia M (EMB), Mshikazi and Delosma H decoctions.Methods: Antioxidant activity of the extracts was evaluated using1-diphenyl-2-picrylhydrazyl (DPPH), glutathione (GSH), phosphomolybdate and thiobarbituric acid reactive substance (TBARS) assays. Phytochemical screening was used to determinethe presence of compounds.Results: The DPPH radical scavenging activity was similar to ascorbic acid for EMB and Delosma H, but not for Mshikazi. At 24 h, EMB increased GSH in both THP-1 and Jurkat cells similar to Delosma H while Mshikazi demonstrated the lowest activity. At 48 h, EMB and Delosma H revealed increased GSH in THP-1 cells with no significant decrease in GSH levels in Jurkat cells. However, EMB showed the lowest lipid peroxidation activity compared to Delosma H and Mshikazi after 24 h treatment of both cells. Phenols, flavonoids, terpenoids, saponins were present in all extracts.Conclusion: Extracts of the three decoctions possess both antioxidant and prooxidant properties through high scavenging activity and increased in lipid peroxidation. Keywords: Antioxidants; herbal medicines; Emelia M; Mshikazi; Delosma H.

scholarly journals Flowers of Astragalus membranaceus var. mongholicus as a Novel High Potential By-Product: Phytochemical Characterization and Antioxidant Activity

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 434 ◽  
Author(s):  
Yuan Li ◽  
Sheng Guo ◽  
Yue Zhu ◽  
Hui Yan ◽  
Da-wei Qian ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Herbal Medicines ◽  
Reducing Power ◽  
Positive Control ◽  
Astragalus Membranaceus ◽  
Reducing Power Assay ◽  
Free Radical Scavenging Assay ◽  
The Individual

The root of Astragalus membranaceus var. mongholicus is one of the most popular herbal medicines worldwide. In order to increase the yield of underground roots of A. membranaceus var. mongholicus, its flowers (AMF) have often been removed in their flowering stage, which produces the flowers as waste being discarded. To explore its phytochemicals and potential value for utilization, the antioxidant activities of extracts from AMF were evaluated by a free radical scavenging assay and reducing power assay. The total phenols and flavonoids, as well as the individual compounds, in different extracts of AMF were also investigated. The results showed that the extract ME obtained from AMF through macroporous resins separation exhibited strong antioxidant activities, which were close to those of positive control BHT. ME was rich in phenolic acids and flavonoids, and the contents reached 108.42 mg gallic acid equivalents/g and 265.70 mg rutin equivalents/g, respectively. A total of 31 compounds, including four phenolic acids, nineteen flavonoids, three isoflavones, two pterocarpans, and three saponins, were identified using UPLC-QTOF-MS in ME. Quantitative analysis of sixteen components in the extracts of AMF showed that flavonoids were the predominant constituents, especially for the compounds of hyperoside, rutin, and isorhamnetin-3-O-β-d-glucoside.

scholarly journals Synthesis And Evaluation Of Herbal Formulation Using Natural Extract

2020 ◽  
Vol 11 (3) ◽  
pp. 3450-3458
Author(s):  
Sudha Rameshwari K ◽  
Muthu Muneeswari M ◽  
Gloria Jemmi Christobel R ◽  
Mallika R ◽  
Renuka R ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Piper Nigrum ◽  
Phytochemical Analysis ◽  
Significant Activity ◽  
Herbal Formulation ◽  
Nitric Oxide Radical ◽  
Anti Inflammatory

Our study is intended to prepare and evaluate the polyherbal formulation (PHF) using Nyctanthes arbor-tristis, Piper nigrum, and lemon juice. Poly Herbal Formulation F1 and F2 were synthesised and subjected to qualitative, quantitative phytochemical analysis and antioxidant activity was measured using a standard protocol. Antimicrobial activity of PHFs was observed by the agar well diffusion method. The anti-inflammatory and anti-diabetic activities (In-vitro) were studied in F1 by using inhibition of albumin denaturation method. TLC and FTIR are done for the structural elucidation in F1. The F1 and F2 exhibited the presence of flavonoids, alkaloids, tannins, phenols, terpenoids, carbohydrates, saponins, Glycosides and proteins. The F1 and F2 show significant activity against the standard in the antimicrobial and antioxidant activity. The presence of flavonoids and alkaloids may contribute to the anti-microbial activity. The Nitric oxide radical scavenging activity and a metal chelating assay of the F1 possess the highest antioxidant activity of 73.1% and 69% respectively. TLC confirms the presence of flavonoids in F1. FTIR spectrum shows the functional group of amines, alcohols, nitrites, aromatic and aliphatic compounds. These FTIR bands denote stretching and vibrational bands responsible for the compounds like flavonoids, terpenoids, phenols and amino acids. This investigation shows the excellent result in antimicrobial, antioxidant and in-vitro anti-diabetic and anti-inflammatory activities. In future, the PHF of F1 can be used as potent therapeutical agents and can be further exploited for their other beneficial effect in the pharmacological studies.

scholarly journals Challenges in Coopted Hydrophilic and Lipophilic Herbal Bioactives in the Same Nanostructured Carriers for Effective Bioavailability and Anti-Inflammatory Action

Nanomaterials ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3035
Author(s):  
Teodora-Alexandra Iordache ◽  
Nicoleta Badea ◽  
Mirela Mihaila ◽  
Simona Crisan ◽  
Anca Lucia Pop ◽  
...  
Keyword(s):  
Physical Stability ◽  
Herbal Medicines ◽  
Morphological Characteristics ◽  
Entrapment Efficiency ◽  
In Vitro Cytotoxicity ◽  
Ongoing Research ◽  
Lipid Nanocarriers ◽  
Anti Inflammatory ◽  
The Individual

There is ongoing research on various herbal bioactives and delivery systems which indicates that both lipid nanocarriers and herbal medicines will be fine tunned and integrated for future bio-medical applications. The current study was undertaken to systematically develop NLC-DSG-yam extract for the improved efficacy of herbal Diosgenin (DSG) in the management of anti-inflammatory disorders. NLCs were characterized regarding the mean size of the particles, morphological characteristics, physical stability in time, thermal behaviour, and entrapment efficiency of the herbal bioactive. Encapsulation efficiency and in vitro antioxidant activity measured the differences between the individual and dual co-loaded-NLC, the co-loaded one assuring a prolonged controlled release of DSG and a more emphasized ability of capturing short-life reactive oxygen species (ROS). NLCs safety properties were monitored following the in vitro MTS ((3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction assay) and RTCA (Real-Time Cell Analysis) assays. Concentrations less than 50 μg/mL showed no cytotoxic effects during in vitro cytotoxicity assays. Besides, the NLC-DSG-yam extract revealed a great anti-inflammatory effect, as the production of pro-inflammatory cytokines (TNF-alpha, IL-6) was significantly inhibited at 50 μg/mL NLC (e.g., 98.2% ± 1.07 inhibition of TNF-α, while for IL-6 the inhibition percentage was of 62% ± 1.07). Concluding, using appropriate lipid nanocarriers, the most desirable properties of herbal bioactives could be improved.

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