THE ROLE OF GENETIC POLYMORPHISM OF THE FILAGGRIN PROTEIN WITH ATOPIC MARCH PROGRESSION IN CHILDREN

The aim of the study. To determine the role of genetic polymorphism in the filaggrin gene R501XAA and 2282de4AA at atopic march progression in children. Materials and methods. 111 children aged 3 to 12 years with atopic dermatitis were selected and examined. As a result of genetic testing, it was found that 51 children with atopic dermatitis had polymorphism in the filaggrin gene. These patients were included in the main group. Another 60 children without polymorphism were in the control group. The filaggrin gene polymorphism was determined by examining the buccal epithelium by Dellaporta method. Sensitization to allergens was established on the basis of the specific IgE level. The impact of the disease on the quality of life of children was performed using the CDLQI questionnaire (Children's Dermatology Life Quality Index). Results. In the course of molecular genetics research, R501X mutation was detected in 40 ((78.4 ± 5.76)%) children, 2282del4 polymorphism – in 4 ((7.8 ± 3.76)%) patients, and their combined variant R501X + 2282del4 – in 7 (13%), (7 ± 4.81)% patients. When determining the effect of filaggrin polymorphism on the clinical course of atopic dermatitis, the presence of the associative relationship was established with the following indicators: the early onset of the disease – χ2 = 33.2, mostly severe course – χ2 = 16.2, severe skin dryness – χ2 = 22.6, predominant sensitization to fungi – χ2 = 10.6 and house dust mites – χ2 = 12.2, violation of the skin microbiome – χ2 = 7.8. Conclusions. Early manifestation of atopic dermatitis in children is associated with the filaggrin protein gene polymorphism ((82.4 ± 5.33)%), which determines the risk of progression of the atopic march and the development of bronchial asthma in (38.0 ± 6.8)% of children.

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The Skin and Gut Microbiome and Its Role in Common Dermatologic Conditions

Microorganisms ◽

10.3390/microorganisms7110550 ◽

2019 ◽

Vol 7 (11) ◽

pp. 550 ◽

Cited By ~ 11

Author(s):

Samantha R. Ellis ◽

Mimi Nguyen ◽

Alexandra R. Vaughn ◽

Manisha Notay ◽

Waqas A. Burney ◽

...

Keyword(s):

Atopic Dermatitis ◽

Environmental Factors ◽

Gut Microbiome ◽

The Body ◽

Skin Microbiome ◽

Disease States ◽

Normal Microflora ◽

The Impact ◽

The Relationship

Microorganisms inhabit various areas of the body, including the gut and skin, and are important in maintaining homeostasis. Changes to the normal microflora due to genetic or environmental factors can contribute to the development of various disease states. In this review, we will discuss the relationship between the gut and skin microbiome and various dermatological diseases including acne, psoriasis, rosacea, and atopic dermatitis. In addition, we will discuss the impact of treatment on the microbiome and the role of probiotics.

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Current understanding of allergic march and the role of eczema in its development

Postępy Higieny i Medycyny Doświadczalnej ◽

10.5604/01.3001.0010.8706 ◽

2018 ◽

Vol 72 ◽

pp. 43-51

Author(s):

Karolina Gwoździewicz ◽

Ewa Cichocka-Jarosz

Keyword(s):

Atopic Dermatitis ◽

Subsequent Development ◽

Childhood And Adolescence ◽

Skin Microbiome ◽

Atopic Diseases ◽

Barrier Dysfunction ◽

Atopic March ◽

Leading Role ◽

Typical Sequence

An increasing morbidity of atopic diseases (atopic dermatitis, food allergy, asthma and allergic rhinitis) documented in large cohort epidemiological studies is at least partially determined by high hygienic standards of living. Over the last 40 years, the accepted concept of pathogenesis of atopic diseases, the so-called atopic march, was proposed by Fouchard in 1973. It referred to the natural history of atopy manifestation, with a typical sequence of symptoms presented as atopic dermatitis in early childhood for subsequent development of allergic respiratory symptoms in late childhood and adolescence. New data suggests that the leading role of atopic dermatitis in atopic march might be less pronounced than previously expected, indicating coexistence rather than succession of atopic symptoms. The objective of this paper is to present the currently discussed concepts of atopic dermatitis – its pathogenesis, etiology, course and role in the development of other allergic diseases. More widely, we will present: 1. The genetic factors involved in skin barrier disruption with the leading role of loss-of-function gene for filaggrin mutation, 2. Genetic defects and epigenetic regulation of the immune system 3. Epidermal changes with physical barrier dysfunction as well as 4. Skin microbiome disturbances with Staphylococcus aureus colonization leading to abnormalities of the epidermal protective barrier.

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Individual and Combined Assessment of Ser680Asn FSH Receptor and FSHβ -211 G>T Gene Polymorphisms in Ovarian Response in IVF/ICSI Program

Current Pharmaceutical Biotechnology ◽

10.2174/1389201021666201029153518 ◽

2020 ◽

Vol 21 ◽

Author(s):

Elli Anagnostou ◽

Alexia Kafkoutsou ◽

Despina Mavrogianni ◽

Ekaterini Domali ◽

Evangelia Dimitroulia ◽

...

Keyword(s):

Gene Polymorphism ◽

Gene Polymorphisms ◽

Ovarian Response ◽

Greek Population ◽

Control Group ◽

Genotype Distribution ◽

Β Subunit ◽

Molecular Genetic Study ◽

The Impact ◽

First Time

Background: Molecular biology tools, such as the detection of single nucleotide polymorphisms (SNPs), have been considered to assist to the management of the ovarian stimulation protocols. Purpose: The aim of this study was to evaluate the impact of two polymorphisms, the Asn680Ser polymorphism of the FSHR gene, and the FSH β subunit (FSHβ) gene polymorphism -211 G>T, in a Greek population of women undergoing IVF/ICSI program in our center. In addition, a control group of fertile women was studied, to verify whether there are differences in the genotype distribution between fertile and infertile population for both polymorphisms, as the FSHβ gene polymorphism -211 G>T is studied for the first time in the Greek population. Results : The FSH β-211 G>T polymorphism, studied for the first time in the Greek infertile population, appears to be quite rare. When studying the two polymorphisms separately, statistically significant differences were obtained that concerned the LH levels. Discussion: According to the combination analysis of the two polymorphisms by the number of alleles, women with 2-3 polymorphic alleles needed more days of stimulation, but there were no differences in pregnancy rates. Conclusion: This molecular genetic study helps to elucidate whether the polygenic combination of the Asn680Ser and FSH β subunit -211 G>T gene polymorphisms is of additive value in the prediction of ovarian response to exogenous gonadotropins.

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Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients

Scientific Reports ◽

10.1038/s41598-021-94624-4 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Maria Clara Saad Menezes ◽

Alicia Dudy Müller Veiga ◽

Thais Martins de Lima ◽

Suely Kunimi Kubo Ariga ◽

Hermes Vieira Barbeiro ◽

...

Keyword(s):

Mechanical Ventilation ◽

Peripheral Blood ◽

World Health ◽

Control Group ◽

Pyrin Domain ◽

Peripheral Blood Gene Expression ◽

Severe Group ◽

Health Organization ◽

The Impact

AbstractThe role of innate immunity in COVID-19 is not completely understood. Therefore, this study explored the impact of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection on the expression of Pattern Recognition Receptors (PRRs) in peripheral blood cells and their correlated cytokines. Seventy-nine patients with severe COVID-19 on admission, according to World Health Organization (WHO) classification, were divided into two groups: patients who needed mechanical ventilation and/or deceased (SEVERE, n = 50) and patients who used supplementary oxygen but not mechanical ventilation and survived (MILD, n = 29); a control group (CONTROL, n = 17) was also enrolled. In the peripheral blood, gene expression (mRNA) of Toll-like receptors (TLRs) 3, 4, 7, 8, and 9, retinoic-acid inducible gene I (RIGI), NOD-like receptor family pyrin domain containing 3 (NLRP3), interferon alpha (IFN-α), interferon beta (IFN-β), interferon gamma (IFN-γ), interferon lambda (IFN-λ), pro-interleukin(IL)-1β (pro-IL-1β), and IL-18 was determined on admission, between 5–9 days, and between 10–15 days. Circulating cytokines in plasma were also measured. When compared to the COVID-19 MILD group, the COVID-19 SEVERE group had lower expression of TLR3 and overexpression of TLR4.

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ANTICIPATORY ABILITY OF COACHES IN THE SYSTEM OF THEIR PROFESSIONAL COMPETENCE EVALUATION BY ATHLETES

SCIENCE AND SPORT: current trends ◽

10.36028/2308-8826-2020-8-3-103-111 ◽

2020 ◽

Vol 8 (3) ◽

pp. 103-111

Author(s):

Lusine Stepanyan

Keyword(s):

Work Experience ◽

Personal Development ◽

Professional Competence ◽

Control Group ◽

Test Results ◽

Perceptual Evaluation ◽

Competence Evaluation ◽

The Subject ◽

The Impact

Current research in psychology and psychophysiology focuses on the subject of anticipation and forecasting. Anticipatory sustainability is a personal trait that determines the ability to anticipate events and possible ways of personal development, as well as the ability to prevent interpersonal conflicts. The aim of the study was to identify the role of development level of anticipatory competence of coaches in their professional competence evaluation and perception by athletes. Research methods and organization. The research involved two groups of test subjects. The experimental group consisted of sport dance coaches and their students. The control group brought together experts in socionomic area with different work experience. We used testing and questionnaire methods to achieve the goal and objectives of the study. Testing method included the test for diagnosing anticipatory sustainability according to A. Mendelevich and the test of socio-perceptual coaching evaluation by athletes. We used the results of testing and questionnaires to provide a comparative and correlation analysis of data. Research results. The analysis of test results revealed the impact of work experience on the development of both spatial and temporal characteristics of the anticipation of coaches, indicating a possible effect on the intensity and direction of development of those characteristics in specific conditions. We have identified valid connections between the gnostic parameter (professional competence evaluation) of the socio-perceptual coaching evaluation by athletes and spatiotemporal characteristics of coaches' anticipation, defined as professionally important qualities of coaches. Moreover, we have demonstrated a weak direct relationship between the personality-situational component of the anticipatory sustainability of coaches and the emotional parameter of their socio-perceptual evaluation by athletes, indicating a significant role of communicative-anticipation sustainability in the development of an attitude of athletes to the coach. Conclusion. In summary, we can conclude that the anticipatory sustainability of coaches plays a key role in the structure of their competence, and the development of this property is possible under certain conditions.

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The role of the skin microbiome in atopic dermatitis

Annals of Allergy Asthma & Immunology ◽

10.1016/j.anai.2018.12.003 ◽

2019 ◽

Vol 122 (3) ◽

pp. 263-269 ◽

Cited By ~ 29

Author(s):

Teruaki Nakatsuji ◽

Richard L. Gallo

Keyword(s):

Atopic Dermatitis ◽

Skin Microbiome

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The Effect of Fixed-Term Employment on Well-Being: Disentangling the Micro-Mechanisms and the Moderating Role of Social Cohesion

Social Indicators Research ◽

10.1007/s11205-020-02421-9 ◽

2020 ◽

Vol 152 (1) ◽

pp. 91-115

Author(s):

Sonja Scheuring

Keyword(s):

Social Cohesion ◽

Well Being ◽

Control Group ◽

Subjective Well Being ◽

Social Contacts ◽

Country Level ◽

Micro Level ◽

Deprivation Model ◽

The Impact

Abstract This paper examines the impact of fixed-term employment on well-being from a cross-national comparative perspective by testing (1) the effect heterogeneity across European countries, (2) to which extent Jahoda’s Latent Deprivation Model provides a sufficient micro-level explanation for the underlying mechanisms and (3) whether the macro-level factor of social cohesion weakens the micro-level impacts. We investigate the effects in both an upwards (permanent employment) and a downwards (unemployment) comparative control group design. Due to the mediating role of social contacts on the micro-level, we assume social cohesion on the country-level to moderate the main effects: A high degree of societal affiliation should substitute the function of social contacts in the work environment of individuals. Using microdata from the European Social Survey (ESS) 2012 for 23 countries and applying multilevel estimation procedures, we find that there is a remarkable variation in the effects across countries. Even though in each country fixed-term employees have a lower subjective well-being compared to permanent ones, the point estimates vary from .17 to 1.19 units. When comparing fixed-term employees to unemployed individuals, the coefficients even range from − .27 to 1.25 units. More specifically, a negative effect indicates that having a fixed-term contract is worse than unemployment in some countries. Moreover, pooled linear regression models reveal that Jahoda’s Latent Deprivation Model explains about three-quarters of the micro-level effect sizes for both directions. Eventually, social cohesion on the country-level diminishes the individual-level well-being differences between fixed-term employees and permanent individuals but not between fixed-term employees and the unemployed.

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Role of bradykinin B1 and B2 receptors in normal blood pressure regulation

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.00382.2005 ◽

2006 ◽

Vol 291 (2) ◽

pp. E268-E274 ◽

Cited By ~ 31

Author(s):

Arvi Duka ◽

Irena Duka ◽

Guohong Gao ◽

Sherene Shenouda ◽

Irene Gavras ◽

...

Keyword(s):

Blood Pressure ◽

Receptor Gene ◽

Normal Blood ◽

Control Group ◽

Normal Blood Pressure ◽

Concurrent Administration ◽

Vasoactive Factors ◽

Hoe 140 ◽

The Impact

With inhibition or absence of the bradykinin B2 receptor (B2R), B1R is upregulated and assumes some of the hemodynamic properties of B2R, indicating that both participate in the maintenance of normal vasoregulation or to development of hypertension. Herein we further evaluate the role of bradykinin in normal blood pressure (BP) regulation and its relationship with other vasoactive factors by selectively blocking its receptors. Six groups of Wistar rats were treated for 3 wk: one control group with vehicle alone, one with concurrent administration of B1R antagonist R-954 (70 μg·kg−1·day−1) and B2R antagonist HOE-140 (500 μg·kg−1·day−1), one with R-954 alone, one with HOE 140 alone, one with concurrent administration of both R-954 and HOE-140 plus the angiotensin antagonist losartan (5 mg·kg−1·day−1), and one with only losartan. BP was measured continuously by radiotelemetry. Only combined administration of B1R and B2R antagonists produced a significant BP increase from a baseline of 107–119 mmHg at end point, which could be partly prevented by losartan and was not associated with change in catecholamines, suggesting no involvement of the sympathoadrenal system. The impact of blockade of bradykinin on other vasoregulating systems was assessed by evaluating gene expression of different vasoactive factors. There was upregulation of the eNOS, AT1 receptor, PGE2 receptor, and tissue kallikrein genes in cardiac and renal tissues, more pronounced when both bradykinin receptors were blocked; significant downregulation of AT2 receptor gene in renal tissues only; and no consistent changes in B1R and B2R genes in either tissue. The results indicate that both B1R and B2R contribute to the maintenance of normal BP, but one can compensate for inhibition of the other, and the chronic inhibition of both leads to significant upregulation in the genes of related vasoactive systems.

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The Impact of Different Teaching Strategies on Teaching Spelling to Kindergarten Children

Journal of Language Teaching and Research ◽

10.17507/jltr.0704.13 ◽

2016 ◽

Vol 7 (4) ◽

pp. 730

Author(s):

Ali Hashemi ◽

Omid Ghalkhani

Keyword(s):

Language Teaching ◽

Kindergarten Students ◽

Kindergarten Children ◽

Control Group ◽

Language Curriculum ◽

The Public ◽

Correct Spelling ◽

The Impact ◽

Arts Community

Many specialists in the field of foreign language teaching consider spelling as an important and perhaps necessary part in language teaching, and observe that spelling is a significant concern among both the language arts community and the public. The teaching of spelling is an issue that provokes strong feelings and attitudes in teachers, students, and parents as well, and those involved have strong opinions about the importance of correct spelling at school. A close look at the role of spelling in the general language curriculum, makes it known that learning to write and read in the whole language can benefit greatly from teaching spelling. Searching the Net under the keywords ‘spelling’ and ‘teaching spelling’ reveals many studies on spelling and the interest of the readers. In this study, we made use of two techniques to teach spelling, which included “the Personalized Instruction Technique”, or simply the PI technique, and “the Detection Technique” or the D technique. Meanwhile, a control group was included to make up for and take into account any possible background differences.82 kindergarten children, studying at three kindergartens in Tarom, Zanjan, took part in the study. They were randomly assigned to three groups: one control group and two experimental groups. The PI group consisted of 28 kindergarten students both boys and girls, and the Group comprised 27kindergarten students, both boys and girls. The control group, too, contained 27 kindergarten students, both boys and girls. The findings indicated that both the experimental groups that used the spelling techniques performed significantly better than the control group. Meanwhile, the PI group exceeded the DT group.

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