scholarly journals PROTECTIVE EFFECTS OF FLAX SEED OIL ON BODY WEIGHT CHANGES CAUSED BY CAFFEINATED ENERGY DRINK IN ADULT MALE ALBINO RATS

2021 ◽  
Vol 31 (04) ◽  
pp. 172-177
Author(s):  
Afifa Waseem ◽  
Muhammad Sohail ◽  
Tayyaba Muzaffar ◽  
Javaid Iqbal ◽  
Hadia Zulfiqar ◽  
...  
Keyword(s):  
Body Weight ◽  
Adult Male ◽  
Seed Oil ◽  
Corn Oil ◽  
Energy Drink ◽  
Albino Rats ◽  
Protective Effects ◽  
Flax Seed ◽  
Group A ◽  
Group B

Flax seed oil has proven dynamic multi systemic effects since ancient times. Consumption of caffeinated energy drinks has also been increased among youth in order to increase mental and physical performance. Due to their widespread usage, hazardous effects on various systems of human body have been reported.    Aims & Objectives: To evaluate the protective effects of flax seed oil on caffeinated energy drink induced changes in adult male albino rat body weight. Place & Duration of Study: This study was conducted in  FPGMI, Shaikh Zayed Hospital Lahore for 8 weeks. Material & methods: 32 adult male albino rats average weight (250-300g) were randomly divided into four groups of 8 animals each. Group A (Control) received corn oil 5ml/kg body weight by gavage in addition to basal diet daily for 8 weeks. Group B (Experimental) were fed on caffeinated energy drink (15ml/kg body weight) and corn oil (5ml/kg body weight). Group C (Experimental) received caffeinated energy drink (15ml/kg body weight) and 40% of flax seed oil (5ml/kg body weight), while group D (Experimental) received caffeinated energy drink(15ml/kg body weight)  and 60% flax seed oil (5ml/kg body weight) daily for 8 weeks respectively. The animals were weighed before and after experiment.  Results: The mean body weight of rats before experiment was insignificant (p = 0.945). After experiment the mean body weight of experimental group B, C and D was increased as compared to control group A, but statistically it was insignificant ( p = 0.319) however, percentage body weight gain was significant( p = 0.003). Conclusion: Flax seed oil alleviated altered body weight caused by caffeinated energy drink in adult male albino rats

scholarly journals ALLETHRIN INDUCED TOXICOPATHOLOGICAL ALTERATIONS IN ADULT MALE ALBINO RATS

2021 ◽  
Vol 5 ◽  
pp. 8-14
Author(s):  
Quratulain Mujahid ◽  
Ahrar Khan
Keyword(s):  
Body Weight ◽  
Adult Male ◽  
Leukocyte Count ◽  
Hemoglobin Concentration ◽  
Time Dependent ◽  
Albino Rats ◽  
Vacuolar Degeneration ◽  
Group A ◽  
Group B ◽  
Group D

This study was designed to investigate the allethrin induced toxico-pathological changes in adult male albino rats. A total of 60 adult male albino rats were divided randomly into 4 equal groups. Group A was treated as control while group B was given Allethrin @ 0.5g/kg, group C @ 1.0g/kg and group D @1.5g/kg body weight respectively, orally once at day 14 of experiment. Dullness, erected hairs, alopecia, less response towards feed and water, watery droppings, tremors, convulsions and coma were more prominent signs in treated groups. Feed intake and body weight was decreased in all treated groups. Acute mortality was seen in group C and D. The total erythrocyte and leukocyte count, hemoglobin concentration and packed cell volume (P≤0.05) was deviated from normal in all the groups treated with allethrin. Histopathologically, kidneys exhibited condensed nuclei, necrotic tubules and congested renal parenchyma. In liver, vacuolar degeneration in nucleus and cytoplasm was observed. Micro nucleated lymphocytes were also evident in group D treated with highest dose of allethrin. In conclusion, allethrin induced dose and time-dependent toxico-pathological effects in adult male albino rats were observed and presented.

scholarly journals Effect of Cleome gynandra leaf extract on the estrous cycle and histology of the ovary and uterus of wistar albino rats

1970 ◽  
Vol 8 (1) ◽  
pp. 1385-1394
Author(s):  
Lemuel Ann Monima ◽  
Muhammad Buhari ◽  
Sodiq Lawal ◽  
Echoru Isaac ◽  
Ssempijja Fred ◽  
...  
Keyword(s):  
Body Weight ◽  
Estrous Cycle ◽  
Wistar Rat ◽  
First Trimester ◽  
Albino Rats ◽  
Average Weight ◽  
Cleome Gynandra ◽  
Group A ◽  
Wistar Albino Rats ◽  
Group B

Cleome gynandra is a medicinal plant that is used all over Uganda to hasten childbirth because, it possesses the ability to contract the uterus. It is also used as an abortifacient in the first trimester. In this study, the effects of Cleome gynandra were investigated on the estrous cycle and the histology of the ovary and uterus of adult Wistar rat. Twelve adult female Wistar rats of 130-140g average weight were used. These were divided into three groups of four animals each. Group A received distilled water only, while animals in groups B and C received 250mg/kg body weight and 500mg/kg body weight of extract, orally and daily respectively. Monitoring of estrous cycle continued throughout the three weeks of extract administration. After three weeks, the ovaries and uteri were excised and processed for histological examination. In the ovary, there was a reduction in number of primordia, primary, secondary and graafian follicles in the treated groups. Vacuolations were common to both the ovarian and uterine tissues of treated animals. The estrous cycle of Group B and C, showed a mild disruption when compared to animals in Group A. The results showed that the plant extract studied, exerted negative influences on the estrous cycle and histology of the ovary and uterus of Wistar albino rats, suggesting a disturbance on the reproductive health of the animals. Further studies to determine the mechanism of action of Cleome gynandra on the ovary and uterus and the levels of FSH, LH, estradiol and progesterone is recommended.Key Words: Cleome gynandra, estrous cycle, Wistar albino rats, ovarian follicles.

scholarly journals The effects of crude extract of Amaranthus hybridus (000059604) on reproductive parameters of the male Wistar rats

2020 ◽  
Vol 47 (4) ◽  
pp. 44-57
Author(s):  
M. O. Oyeyemi ◽  
V. B. Adedeji ◽  
A. M. Bankole
Keyword(s):  
Body Weight ◽  
Antioxidant Properties ◽  
Albino Rats ◽  
Reproductive Parameters ◽  
Amaranthus Hybridus ◽  
Treatment Groups ◽  
Wistar Strain ◽  
Male Wistar Rats ◽  
Group A ◽  
Group B

Studies on Amaranthus hybridus (AH) have established its antioxidant properties. This study investigated the effects of AH on some reproductive parameters of forty-five sexually matured Albino rats (Wistar Strain) males. Amaranthus hybridus has been reported to have several protective and curative properties attributed majorly to strong antioxidant activity. They were randomly grouped into 3 with each group consisting of 15 rats. Group A received (per Os) Amaranthus hybridus (50mg/kg body weight) and group B had Amaranthus hybridus (100mg/kg body weight for 7 days. Group C (control) received distilled water for 7 days. Blood and semen samples were collected for analyses on day 1,8,15 and 22 post-treatments. The results showed that the sperm motility of group B in day 1, (2.00± 2.00%) rat was significantly lower (p < 0.001) than that of group C (26.00 ± 7.97%), whereas it was greatly improved in Group B (85.00 ± 4.47%) and Group A (60.00 ±4.47%) at the day 22 posttreatment. Livability of sperm cells were significantly higher (p < 0.5 0) in Group A (96.20±0.73%) and Group B (96.20±0.73%) compared to Group C (96.80±1.64%) at day 22 post-treatment. The histology of the testis of control and all treatment groups revealed normal testicular germinal epithelium full of viable spermatogenic cells. It is, therefore, concluded that Amaranthus hybridus extract given orally at 50mg/kg and 100mg/kg body weight, did not affect the normal cytoarchitecture of the testis and epididymis and can increase sperm production.

scholarly journals Protective Effects of Ocimum basilicum Against Atrophic Changes in Graafian Follicles in Cyclophosphamide Induced Ovarian Toxicity

2021 ◽  
Vol 35 (1) ◽  
pp. 33-38
Author(s):  
Saba Saleem
Keyword(s):  
Ocimum Basilicum ◽  
Medical Institute ◽  
Control Group ◽  
Albino Rats ◽  
P Value ◽  
Protective Effects ◽  
Intraperitoneal Dose ◽  
Group A ◽  
Group B ◽  
Natural Herb

Introduction: Cyclophosphamide is one of the alkylating chemotherapeutic drug used in cancer patients that has antifertility effects on female gonads. Ocimum basilicum is a natural herb rich in polyphenols and is known to improve fertility. Aims & Objectives: The study was designed to evaluate the role of natural herb, Ocimum basilicum extract, as a preventive agent against ovarian follicular toxicity induced by cyclophosphamide. Place and duration of study: This experimental study was performed in the Department of Anatomy, Shaikh Zayed Postgraduate Medical Institute, Lahore. The duration of study was 8 months. Material & Methods: 45 female albino rats were divided equally in control group A, experimental group B and group C each contained 15 rats. Group A rats received single dose of 150 mg/kg normal saline intraperitoneally on 8th day of experiment, while group B was given single intraperitoneal dose of 150 mg/kg cyclophosphamide at day 8 of experiment. Group C rats were pretreated with methanolic basil (Ocimum basilicum) seeds extract for 7 days followed by single intraperitoneal dose of 150 mg/kg cyclophosphamide at day 8 of experiment. All the rats were dissected 48 hours after the last dose. Results: Graafian follicles were atrophied showing atretic granulosa cells in group B when compared with control group A with p value <0.001. However, significant improvement in status of Graafian follicles was observed in group C, when compared with group B with p value <0.025. Conclusion: This study depicts that basil seeds extract can prevent the cellular toxicity in Graafian follicles caused by cyclophosphamide treatment. So the use of basil seeds during chemotherapy can significantly limit its toxic effects on Graafian follicles.

scholarly journals CELECOXIB

2018 ◽  
Vol 25 (01) ◽  
pp. 50-57
Author(s):  
Sadia Sundus ◽  
Nazia Qamar ◽  
Raheela Adil ◽  
Muhammad Faisal Fahim
Keyword(s):  
Body Weight ◽  
Relative Weight ◽  
Treated Group ◽  
Renal Tissue ◽  
The Body ◽  
Control Group ◽  
Albino Rats ◽  
Group A ◽  
Group B ◽  
General Architecture

Objective: To observe the absolute, relative weight of kidney and body weightof albino rats on celecoxib induced kidney with protection by lycopene. Study Design:Experimental study. Place and Duration of study: This study was conducted in BMSI (Anatomydepartment), JPMC, Karachi, from 4th May 2015 to 3rd June 2015. Materials and Methods: Fortyhealthy adult, male Albino rats, 90-120 days old, weighing 200-220gm was taken for the study.The rats were divided into 4 groups, Group A was control group, Group B receive Celecoxib 50mg/kg body weight orally, Group C receive Celecoxib 50 mg/kg body weight orally along withlycopene50 mg/kg body weight orally and Group D receive lycopene 50 mg/kg body weightorally for 30 days. At the end of study rats were sacrificed and renal tissue sections were stainedwith hematoxylin and eosin. Results: Markedly decreased weight was observed in rats takingcelecoxib. Slides which were stained with hematoxylin and eosinshowed general architecture ofrenal parenchyma, shape and arrangement of epithelial cells. Apoptosis, hemorrhage, necrosisand vacuolation seen in Celecoxib group, whereas renal architecture were ameliorated andreverted back in celecoxib along with lycopene receiving group. Conclusion: This studyconcludes that lycopene restored the body weight, absolute and relative kidney weight incelecoxib treated group.

scholarly journals PROTECTIVE EFFECT OF SEA BUCKTHORN SEED OIL EXTRACT ON ACETAMINOPHEN INDUCED HISTOLOGICAL CHANGES OF HEPATOCYTES IN ADULT ALBINO RATS.

2021 ◽  
Vol 32 (01) ◽  
pp. 09-14
Author(s):  
Annum Basit ◽  
Attya Shahid ◽  
Shagufta Nasreen
Keyword(s):  
Protective Effect ◽  
Seed Oil ◽  
Sea Buckthorn ◽  
Medical Institute ◽  
Albino Rats ◽  
Adult Rats ◽  
Group A ◽  
Oil Extract ◽  
Group B ◽  
Group D

Objective: to reveal the effects of sea buckthorn seed oil on acetaminophen induced variations (Diameter of hepatocytes and vacuolization) in liver of rats. Methods: it was experimental study, conducted at Post Graduate Medical Institute. This study was performed on 24 adult rats, which were then randomly separated into 4 groups A,B,C and D. the experiment lasted for 13 days. Group A was control received normal saline and distilled water. Group B, received acetaminophen750mg/kg as single dose on day 10. Group C and D received acetaminophen at dose of 750mg/kg along with sea buckthorn seed oil extract 2.6mg/kg and 5.2mg/kg. Liver was removed and fixed in 10% formalin. To observe the effect of acetaminophen and sea buckthorn seed oil , slides were prepared for histological examination. The diameter of hepatocytes and vacuolization was observed. The evaluation of results was done by using SPSS 21. Results: In Group B, vacuolization was seen in all animals and significant increase in mean diameter (11.9± 1.5 μm) of hepatocytes was seen. However in Group C, vacuolization was absent in all animals and mean hepatocyte diameter was (6 ± 0.6 μm). In Group D, no vacuolization was seen in all animals and mean hepatocyte diameter decreased to (5.2± 1.5 μm) after administration of SBT seed oil. Conclusion: Administration of SBT( 2.6mg/kg and 5.2mg/kg) exerts a protective effect against damaging effects of ACM ( 750 mg/kg) on hepatocytes in albino rats. This hepatoprotective role becomes greater with increasing dose of SBT.

scholarly journals Histoprotective effects of Curcumin on Bisphenol- A induced testicular toxicity in adult albino rats.

2019 ◽  
Vol 26 (12) ◽  
pp. 2206-2215
Author(s):  
Rabia Sajjad Toor ◽  
Faiza Irshad ◽  
Sania Asif
Keyword(s):  
Weight Gain ◽  
Basement Membrane ◽  
Bisphenol A ◽  
Corn Oil ◽  
Membrane Integrity ◽  
The Body ◽  
Albino Rats ◽  
Group A ◽  
Group B ◽  
Group D

Endocrine Disrupting chemicals including bisphenols have contaminated the environment significantly and is known to damage spermatogenesis via increase in oxidative stress. The anti-oxidant properties of naturally occurring substances like curcumin have been well established. Objectives: To evaluate the protective effects of curcumin against bisphenol-A induced testicular damage. Study Design: Experimental Study. Setting: Department of Anatomy, University of Health Sciences, Lahore. Period: From March 2015 to December 2015. Material & Methods: Thirty six rats were divided into four groups A, B, C and D of 9 animals each. Group A (control) was given 5ml/kg/day of corn oil orally for 10 days. Group B (Bisphenol-A) was given 100mg/kg/day of Bisphenol-A dissolved in corn oil orally for 10 days. Group C (recovery) was given 100mg/kg/day of BPA dissolved in corn oil orally for 10 days and left untreated for 10 days. Group D (Curcumin + BPA) was given 100mg/kg/day of BPA and 100mg/kg/day of Curcumin, dissolved in corn oil orally for 10 days. Rats of group A, B and D were sacrificed on day 11 and those of group C on day 21. After weighing the euthanized rats, testes were removed, processed and tissue sections were stained with H&E for Johnson scoring and with PAS stain for assessment of basement membrane integrity. Results: Bisphenol-A administration caused a significant decrease in weight of animals, a significantly low Johnson score of seminiferous tubules and high frequency of disrupted basement membranes of the tubules in Group B as compared to control. The weight gain of animals improved in the with-drawl group C while no self-recovery was observed in other parameters. Curcumin co-administration improved the body weight gain of animals, increased the Johnson scoring of tubules significantly and partially restored the basement membrane integrity in group D, comparable to the control group. Conclusion: The results of this study indicate that co-administration of a potent antioxidant curcumin causes a significant antagonism of the histo-toxicity of testis produced by Bisphenol-A in albino rats.

scholarly journals Effects of Moringa oleifera lam. Leaf Powder on Bifidobacteria and Escherichia coli in the Gut of Albino Rats

2019 ◽  
pp. 1-11
Author(s):  
A. O. Gbadebo ◽  
O. T. Okareh ◽  
A. A. Ogunjobi ◽  
A. O. Dada
Keyword(s):  
Escherichia Coli ◽  
Body Weight ◽  
Moringa Oleifera ◽  
Albino Rats ◽  
Control Groups ◽  
Moringa Oleifera Leaves ◽  
Leaf Powder ◽  
Group A ◽  
Group B ◽  
Animal House

Aim: This study was carried out to determine the effects of dried Moringa oleifera leaves on Bifidobacteria and Escherichia coli in the gut of albino rats. Location: The rats were habituated under laboratory conditions at the animal house of the Department of Zoology, Faculty of Science, University of Ibadan, for two weeks in other to adapt to the environmental conditions during the experiment. Duration of Study: The rats were exposed to the M. oleifera feed for four weeks. Design of Study: There were five groups in all. The 5 to 6 weeks old rats were fed with M. oleifera powder supplement except for the control groups. Methods: No supplement of M. oleifera feed was administered to group A while group B received streptomycin antibiotics. Groups C, D and E received dried leaf supplement of M. oleifera (DMO) 1.25 g/kg body weight (2.5%), 2.5 g/kg body weight (5%) and 5.0 g/kg body weight (10%) respectively. Results: E.coli counts increased from 2.3*104 to 2.6*104 colony-forming units per gram (cfu/g) in group E, from 2.2*104 to 3.0*10 cfu/g in group B; but reduced from 4.1*104 to 3.7*104 cfu/g in group D and from 5.4*104 to 3.9*104 cfu/g in group C between day 20 and day 28. As from day 8, the isolates from the non-control groups were resistant to the M. oleifera extract except E. coli isolates in both 5% and 10% M. oleifera groups on day 8 with 6 mm zone of inhibition each. The rate of Bifidobacteria viable counts increase in group E was expressed as P = 0.05 at the beginning of the experiment, unlike E.coli counts where there was a decrease. Conclusion: The M. oleifera leaf alters the microbiota in the gut, a situation which sends impulses to the brain. Thus, the M. oleifera leaf powder is a potential prebiotic for probiotics like Bifidobacteria, and as well as induce changes in the gut-brain axis.

scholarly journals Attenuation of The Protective Effect of Honey On Alcohol Induced Hepatotoxicity By Kolanut In Male Albino Rats

2020 ◽  
Author(s):  
Adeoye Waheed Saka ◽  
Busuyi David Kehinde ◽  
Adebola Olayemi Akintola ◽  
Samuel Seyi Oyekanmi ◽  
Ajoke Esther Oyewande ◽  
...  
Keyword(s):  
Liver Damage ◽  
Male Rats ◽  
Albino Rats ◽  
Histopathological Analysis ◽  
Protective Effects ◽  
Group A ◽  
Access To Water ◽  
Group B ◽  
Hepatic Markers ◽  
Better Than

Abstract Background: The excessive use of alcohol can lead to alcohol liver damage. However, natural compounds such as honey and kolanut have been investigated to have hepatoprotective activities against liver damage. Objectives: The possible protective effects of aqueous extract of kolanut and honey and the combination of both on alcohol induced hepatotoxicity of male rats were investigated.Materials and Methods: The rats were randomly divided into five equal groups (n=6). Group A was allowed access to water; Group B received ethanol (45% at 3g/kg) once for 21 days, Group C, D and E received alcohol an hour before treated with 50mg/kg of Cola nitida, 2.5g/kg of honey and combination of both respectively. Histopathological analysis and measurement of serum alanine aminotransferase (ALT), aspartate amino transferase (AST), alkaline phosphatase (ALP), malondialdehyde (MDA) and catalase (CAT).Results: Administration of ethanol (45%) at 3g/kg to rats resulted in significant (P < 0.05) increase in the activities of hepatic markers enzymes (ALT, AST, ALP) as compared with control. Pretreatment of rats with kolanut (50 mg/kg) and honey (2.5g/kg) separately caused significant reduction in ALT, AST and ALP activities while pretreatment with combination of both was not better than the decrease observed when pretreated separately as compared to alcohol-induced group. Conversely, the results of oxidative stress and histological findings took a different pattern in all the pretreated groups.Conclusion: The result showed that the combination of honey and kolanut does not work synergistically in alcohol-induced hepatic damage.

scholarly journals TESTICULAR VOLUME

2015 ◽  
Vol 22 (10) ◽  
pp. 1356-1361
Author(s):  
Hina Khan ◽  
Mazhur-Ul- Haque ◽  
Muhammad Rafique ◽  
Asad Raza Jiskani
Keyword(s):  
Body Weight ◽  
Male Fertility ◽  
Petri Dish ◽  
Health Sciences ◽  
Albino Rats ◽  
Sperm Cells ◽  
Group A ◽  
Study Institute ◽  
The Mean ◽  
Group B

Objective: To determinate that male fertility influenced by testicular volumein albino rats. Study Design: Experimental. Place and Duration of study: Institute of BasicMedical Sciences, Dow University of Health Sciences, Karachi, 10 months (November 2009to August 2010). Methodology: Sixty four adult albino rats were obtained from animal houseJinnah Postgraduate Medical Centre for the study and divided into 2 groups. Group A receivedinjection normal saline 1 cc intraperitoneally (IP) daily for 8 weeks. Group B received leadchloride in a dose of 10 mg/kg body weight IP daily. On the day of completion of treatmentthe animals were sacrificed testes along with epididymis removed and place in Petri dish. Thelength, breath and width of testes were measured with help of vernier caliper. The spermatozoawere obtained from cauda epididymis. Results: The mean ± SEM of volume testes in groupA and B after eight week of treatment were 0.77142 ± 0.04778 cm3 & 0.11768 ± 0.01673 cm3respectively. The volume of testes of group B was significantly decreased as compare to groupB (P = 0.000). Mean ±. The mean ± SEM number of sperm cells million / ml in groups A andB after eight week of treatment was 7.65 ± 186706.553 & 1.84 ± 132792.770 respectively.Number of sperms in group B were significantly decreased as compared to group A (P =0.000). Conclusion: There was relationship between volume of testes and male fertility.

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