Effects of diet fortification with Azadirachta indica and Moringa oleifera leaf meal on parasitaemia, performance, rectal temperature and serum biochemistry of rabbits infected with Trypanosoma brucei brucei

2021 ◽  
Vol 48 (2) ◽  
pp. 101-110
Author(s):  
A. O. Salifu ◽  
S. N. Carew ◽  
A. J. Dadah ◽  
M. Adamu

Numerous plants are reported to have ethno-therapeutic properties; among these plants are the leaves of Moringa oleifera and Azadirachta indica used for the treatment of protozoan infections. Forty grower rabbits at approximately 70 days old were used to determine the prophylactic anti-trypanosomal effects of the dry leaf meals of Moringa oleifera (MLM) and Azadirachta indica (NLM). The rabbits were placed into eight groups of five animals each in a completely randomized design, with mean live weights of 1.2 Kg. The rabbits were allotted to four diets (D1, D2, D3 and D4) and two levels of inoculation (inoculated and uninoculated rabbits) in a 4×2 factorial arrangement of a Completely Randomised Design. Parameters monitored were performance, serum biochemistry and rectal temperature. The parasitaemia were presented as line graph for the infected rabbits. The percent mortality of the groups was evaluated and no mortality was observed for rabbits fed D4. No significant differences (P>0.05) were observed between treatments for growth rate, feed intake and feed conversion, except rectal temperature and total protein (P<0.05) in serum biochemistry. Rabbits that were uninfected with trypanosomes had (P<0.05) lower total protein than the infected animals except T2. It was concluded that only the 1% MLM had prophylactic anti- trypanosomal effect on serum total protein and mortality amelioration of T. b. brucei infected rabbits when administered alone and a combination of both MLM and NLM prevented  pyrexia and mortality.     De nombreuses plantes auraient des propriétés ethno-thérapeutiques ; parmi ces plantes figurent les feuilles de Moringaoleifera et d'Azadirachtaindica utilisées pour le traitement des infections protozoaires. Quarante lapins de culture à environ 70 jours ont été employés pour déterminer les effets anti-trypanosomal prophylactiques des repas secs de feuille de Moringaoleifera (le 'MLM') et d'Azadirachtaindica (le 'NLM'). Les lapins ont été placés en huit groupes de cinq animaux chacun dans une conception complètement randomisée, avec des poids vivants moyen de 1,2 kg. Les lapins ont été attribués à quatre régimes (D1, D2, D3 et D4) et deux niveaux d'inoculation (lapins inoculés et non ininoculés) dans un arrangement factorial de 4×2 d'un Désigne complètement randomisé. Les paramètres surveillés étaient la performance, la biochimie sérique et la température rectale. La parasitémie a été présentée comme graphique linéaire pour les lapins infectés. La mortalité en pourcentage des groupes a été évaluée et aucune mortalité n'a été observée chez les lapins nourris au D4. Aucune différence significative (P>0,05) n'a été observée entre les traitements pour le taux de croissance, l'apport alimentaire et la conversion des aliments pour animaux, à l'exception de la température rectale et de la protéine totale (P<0,05) dans la biochimie sérique. Les lapins qui n'étaient pas infectés par les trypanosomes avaient (P<0,05) moins de protéines totales que les animaux infectés, à l'exception du T2. On l'a conclu que seulement le MLM de 1% a eu l'effet anti-trypanosomal prophylactique sur la protéine totale de sérum et l'amélioration de mortalité des lapins infectés de T.b. brucei une fois administrés seuls et une combinaison de MLM et de NLM a empêché la pyrexie et la mortalité.

2008 ◽  
Vol 5 (3) ◽  
pp. 357-361
Author(s):  
Baghdad Science Journal

This study was conducted in the Poultry farm of the animal during the Production department, Iraqi during the (Ministry of Science and Technology) period from 3-9-2001 to 8-4-2002. The objectives of this study were to evaluate the effect of low – level chronic aflatoxicosis on performance (body weight, feed conversion efficiency and mortality), Serum biochemistry and activity of some enzymes (GOT,GPT, ALKP, LDH). A total of 300 male chicks of broiler breeder (Faw–Bro) were used. Chicks at day 1 of age were fed diets contaminated with aflatoxine at levels of 0, 0.3, 0.6, 0.9, 1.2, and 1.5 the feeding period were extended to 8 weeks. The data were subjected to analysis of variance by the completely randomized design. The results showed the presence of a number of fungi especially the type of Aspergillus flavus genus that reaches 40%. All aflatoxine dose levels significantly (P < 0.05) decreased live weight, it was also noticed that there were higher significant decrease in feed conversion when aflatoxine level was elevated in the feed. Increasing mortality with each increasing in aflatoxine level, the low level of chronic aflatoxine in the diets caused increased in the relative weight of internal organs (Liver, Pancreases, spleen, heart, gizzard, fibresia gland) as a results of aflatoxine presence in the diet, the low level of chronic aflatoxicosis for long time decreased in packed cell volume percentage, heamoglbulin concentration and red blood cell cont., with increase in white blood cells and (neutrophil, Hetrophil, Lymphocyte). The aflatoxine increased the serum concentration of protein, albumin, Cholesterol, Phosphor the activity of LDH, GOT, GPT, AIKP.


Author(s):  
Edi Erwan ◽  
Santika Yulia Wulandari ◽  
Evi Irawati

Abstract  Moringa leaves (Moringa oleifera Lam) is one of the potential local feed ingredients of broiler rations due to its nutrient contents such as carbohydrate (57,01%), crude protein (30,30%), crude fat (2,74%) as well as a source of vitamin C, carotenoids and flavonoids. This study aimed to determine the effect of using different levels of Moringa oleifera Lam leaf meal (MOLM) in basal ration on plasma metabolites, including total cholesterol (TCHO), triglycerides (TG), glucose (GLU), and total protein (TP). The research design of this study was a completely randomized design (CRD) with four treatments and five replications. The treatments were 4 levels of MOLM (0%, 5%, 10% and 15%), in basal ration. The parameters measured were TCHO, TG, Glu, and TP of in blood plasma. The results showed that inclusion of MOLM up to 15% of in basal ration very significantly (P<0.01) lowering levels TG and decreasing Glu. However, the inclusion of MOLM had no effect (P>0.05) on TCHO and TP. It is concluded that moringa leaf flour addition to the basal ration up to 15% could be used to decrease plasma metabolites especially TG and GLU in broiler chickens.  Key words:Broilers; Moringa oleifera meal; total cholesterol, triglycerides, glucose  and total protein   Abstrak  Daun kelor (Moringa oleifera Lam) merupakan salah satu bahan pakan lokal yang berpotensi dapat dimanfaatkan sebagai bahan penyusun ransum ayam ras pedaging karena mengandung nutrisi yang tinggi diantaranya karbohidrat (57,01%), protein kasar (30,30%), lemak kasar (2,74%) dan sebagai sumber vitamin C, karotenoid serta flavonoid. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan beberapa level tepung daun kelor  dalam ransum basal terhadap plasma metabolit ayam ras pedaging yang meliputi total kolesterol (TCHO), trigliserida (TG), glukosa (Glu) dan total protein (TP). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan empat perlakuan dan lima ulangan. Perlakuan yang diberikan terdiri dari 4 level tepung daun kelor di dalam ransum basal yakni 0%, 5%, 10% dan 15%. Parameter yang diukur meliputi TCHO, TG, Glu dan TP pada plasma darah. Hasil penelitian menunjukkan bahwa pemanfaatan tepung daun kelor hingga 15% dalam ransum berpengaruh nyata (P<0,05) menurunkan kadar TG dan berpengaruh sangat nyata (P<0,01) menurunkan Glu. Akan tetapi, pemanfaatan tepung daun kelor hingga 15% tidak berpengaruh nyata (P>0,05) terhadap TCHO dan TP. Kesimpulan penelitian ini adalah penggunaan tepung daun kelor dalam ransum basal hingga 15% dapat dimanfaatkan untuk menurunkan plasma metabolit khususnya TG dan Glu pada  plasma darah ayam broiler.   Kata kunci : Ayam ras pedaging; tepung daun kelor; plasma metabolit


2020 ◽  
Vol 47 (2) ◽  
pp. 131-143
Author(s):  
O. S. Akinola ◽  
K. A. Akintola ◽  
O. O. Oluwatosin

Fishmeal, as animal protein source, is characterised by high cost, dwindling availability and adulteration by feed ingredient suppliers. In view of these, two hundred and ten (210) ten-day old broiler chicks were used, in 2 x 4 experimental layouts, to investigate the effect of replacing fishmeal with infertile egg meal of two forms (with or without shell) and at four graded levels (0, 30, 60 and 100%) on growth performance, haematology, serum biochemistry and carcass indices of broiler chicken. Crude protein (50.2%) and Ether extract (30.6%) were higher in infertile egg without shell (IEM) than in infertile egg withshell (IEMS) (41.36 and 23.4%). Egg forms (IEM or IEMS) did not affect (P>0.05) performance indices measured. Increasing levels of infertile egg meal reduced (P<0.05) body weight gain and final weight without affecting feed conversion ratio (FCR). The IEMS use led to reduction in feed cost (P<0.05) than in IEM. Levels of infertile egg meal reduced (P<0.05) feed cost per body weight gain with least cost at 100% replacement of fishmeal. The IEM elevated (P<0.05) serum total protein, urea and globulin but lowered serum creatinine. Increasing the levels of infertile egg meal lowered blood urea while increasingtotal protein and globulin. Inclusion of IEM at 100% improved Total protein, Albumin and Globulin. The cost of feed reduced (P<0.05) by 13.8% at 100% IEM and IEMS. It was concluded that infertile egg without shell can be used up to 100% in place of fishmeal in broiler chicken diets to reduce feeding cost and improve health without affecting the performance. 


2018 ◽  
Vol 76 (4) ◽  
pp. 174-182 ◽  
Author(s):  
Ibrahim Adeshina ◽  
Ramatu Abdulkareem Sani ◽  
Yusuf Adetunji Adewale ◽  
Lateef Oloyede Tiamiyu ◽  
Samuel Bem Umma

Abstract The growth performance, nutrient utilization and health status of Cyprinus carpio fed various levels of Moringa oleifera leaf meal as a replacement for soybean meal was investigated. Six isonitrogenous diets were formulated with Moringa oleifera leaf meal at 0%, 10%, 20%, 30%, 40% or 50% crude protein replacement. The diets were fed to the fish at 5% body weight to 360 Cyprinus carpio juveniles (8.12±0.21 g) allotted to 18 happas (1 m3) in a completely randomized design for 12 weeks. The results revealed that crude protein replacement levels of 30% had significantly better final weight, weight gain, specific growth rate, protein efficiency ratio and feed conversion ratio, while survival rates were not significantly different. Also, haematological, biochemical and immune responses of the fish fed Moringa oleifera leaf meal fortified diets were significantly improved. The results further suggest that higher inclusion replacement is possible but opined that, for growth and economic consideration, Moringa oleifera leaf meal could be used to replace 30% crude protein of soybean in the diet of Cyprinus carpio juveniles.


Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1116
Author(s):  
Ahmed A. A. Abdel-Wareth ◽  
Jayant Lohakare

This study investigated the dietary effects of Moringa oleifera leaves supplementation on egg quality, laying performance, excreta ammonia concentrations and serum biochemistry of laying chickens during the late laying period. A total of 240 64-week-old Hy-Line Brown hens were assigned to four treatment diets including Moringa oleifera leaves at 0, 3, 6 or 9 g/kg, respectively, for eight weeks. The treatments had twelve replicates with five hens each. The results revealed that incremental dietary Moringa oleifera leaves significantly increased (p < 0.01) egg weight, production, and mass through 64–68, 68–72 and 64–72 weeks of age. Simultaneously, feed conversion ratio was significantly improved (p < 0.01) with Moringa oleifera leaves supplementation compared with the control. Haugh units and the thickness of eggshells significantly improved as a response to diets supplemented with 3, 6 and 9 g/kg Moringa oleifera leaves at 72 weeks of age. Interestingly, excreta ammonia concentrations, serum cholesterol, aspartate transaminase and alanine aminotransferase significantly decreased by Moringa oleifera leaves supplementation compared with the control group. In conclusion, introducing Moringa oleifera leaves supplementation at 3, 6 and 9 g/kg increased egg production, eggshell quality, Haugh units, and decreased serum cholesterol, triglycerides, excreta ammonia concentrations besides serum liver enzymes, uric acid and creatinine. Overall, based on the observed results, Moringa oleifera leaves supplementation was very promising and these leaves could be used as an effective feed additive in laying hens’ diet during the late laying period.


2020 ◽  
Vol 44 (4) ◽  
pp. 177-183
Author(s):  
B. O. Adetoro ◽  
O. O. Olusola ◽  
E. A. Iyayi ◽  
T. A. Olaniyi ◽  
O. Y. Adedeji ◽  
...  

Blood reflects the pathological status of animals exposed to toxicants and other conditions. Blood examination helps to clinically investigate the presence of metabolites and other constituents in the body of animals and plays vital roles in the physiological, nutritional and pathological status of animals. Blood constituents change in relation to the physiological status of an animal.A total of 144 one-day-old Isa-brown pullet chicks were randomly picked from a larger flock, weighed and distributed to groups with 4 replicates of 9 chicks each in a completely randomized design (CRD). Four feeds were formulated to meet the NRC requirement and allotted one each to the 4 group of birds. T1 was the control, T2, T3 and T4 contained 2% of Moringa oleifera, Ocimum gratissimum and Vernonia amgydalina leaf meals respectively. At the end of the experiment which lasted for 8 weeks 2 birds were randomly selected from each replicate for haematological and serum boichemical examination. All the haematological parameters measured were not significant (P>0.05) except eosinophils while for serum biochemistry cholesterol and albumin to globulin ratio were significantly affected by dietary treatment ( P<0.05).The highest value (3.50 % eosinophils) was observed in the birds on diet 4 which had 2% of Vernonia amgydalina leaf meal and these birds also had the lowest (139.25 mg/dl) cholesterol value. It was therefore concluded that 2% of these leaf meals especially of Vernoniaamgy dalinacanbe incorporated in the diets of pullet chicks to reduce their blood cholesterol and boost the immunity


2020 ◽  
Vol 5 (2) ◽  
pp. 55
Author(s):  
Yuda Saputra ◽  
Syahrizal Syahrizal ◽  
Safratilofa Safratilofa ◽  
Edy Barkat Kholidin

The purpose of this study was to determine the effect of giving Moringa oliefera L leaf meal through feed as an anti-bacterial to prevent infection with E.ictaluri bacteria in catfish (Pangasius hypopthalmus). This research was conducted from March 2020 to April 2020 at the Fish Health Laboratory, Freshwater Aquaculture Fisheries Center (BPBAT) Jambi. The research method used was a completely randomized design with five treatments and three replications. Catfish were fed for 14 days with treatment feed with each treatment (A. 5% / kg feed), (B. 10% / kg feed), (C. 15% / kg feed), (D. Commercial feed ) and (E. Commercial feed). and on the 16th day the fish were injected with the Edwarsiella ictaluri bacteria, and survival was observed from day 16 to day 26. The results showed that treatment of moringa leaf meal (C. 15% / kg of feed), could prevent bacterial infection E. ictaluri in catfish with the highest survival rate reaching 66.67%, fish growth of 5.78 grams / head, and feed conversion of 0.18% / kg of feed.


2020 ◽  
Vol 9 (8) ◽  
pp. e828986702
Author(s):  
Dayane Josiane Vieira ◽  
Sandra Regina Freitas Pinheiro ◽  
Keila Abadia Barbosa ◽  
Danielly Rodrigues Fernandes Moreira ◽  
Leilane Rocha Barros Dourado ◽  
...  

This study aimed to evaluate the performance of broilers fed a diet in which the total protein has been replaced by the protein of the crambe meal. The experiment was used 630 male chickens (Cobb® 500) from 8 to 42 days of age, distributed in a completely randomized design with five treatments, six replications of 21 birds per plot. The treatments were: control diet (0%) and diets containing 3, 6, 9 and 12% of crambe meal protein in replacement of the total protein of the feed. The performance was evaluated in the initial phase (8 to 21 days), growth (22 to 42 days) and total (8 to 42 days). Carcass and cuts was evaluated at 43 days of age. Diets containing crambe meal promoted lower feed intake compared with to the control diet in all evaluated phases. For weight gain, a worse result was observed with increased inclusion of crambe protein, with a linear decrease compared to those who did not receive crambe in the total breeding period. It was found that for the total period the best feed conversion was obtained with 6% of the inclusion of crambe protein in comparison to the crambe-free diet. Carcass, breast and thigh weights decreased with a diet containing 12% of the crambe protein. The results indicate that it is possible to partially replace the crude protein of the feed up to 6% with the protein of the crambe meal.


2020 ◽  
Vol 45 (5) ◽  
pp. 30-38
Author(s):  
R. Audu ◽  
A. Tijjani ◽  
A. A Ibrahim ◽  
A. B. Amin ◽  
I. A. Gumel ◽  
...  

A study was conducted to investigate the effect of varying levels of Ficus sycomorus and Parkia biglobosa leaf meals based diets on the haematology and serum biochemistry of weaner rabbits. Twenty rabbits of mixed breed and sex were allotted to four treatments with each treatment consisting of five rabbits per treatment. The treatments consisted of diets with zero forage (T; control), 5% Ficus sycomorus leaf meal (T), 5% Parkia biglobosa leaf meal 1 2 (T) and 2.5% Ficus sycomorus + 2.5% Parkia biglobosa leaf meals (T) in a completely 3 4 randomized design. The diets were iso-nitrogenous and the trial lasted for eight weeks. Haematological parameters measured were PCV, Hb, RBC, WBC, MCV, MCH and MCHC whereas total protein, albumin, globulin, glucose, urea and cholesterol were the biochemical parameters measured. Result of the study showed that Ficus sycomorus and Parkia biglobosa leaves were high in DM, CP, CF, fat and ash contents (94.50% and 92.30%, 18.66% and 19.60%, 9.32% and 7.12%, 9.71% and 8.00%, 10.46% and 12.60%, respectively) but low in moisture (5.50% and 7.70%, respectively). It also revealed significant differences (P<0.05) in all parameters evaluated for haematology [31.93 - 6 3 6 341.90% PCV, 9.50 - 12.10 g/dL Hb, (4.13 - 5.93) x 10/mm RBC, (1.71 - 5.80) x 10/mm WBC, 70.67 - 77.37 Fl MCV, 20.46 - 23.96 Pg MCH and 29.00 - 30.00% MCHC]. The values of total protein (68.00 - 74.00 g/L), albumin (34.00 - 48.33d/L), globulin (25.67 - 34.00g/L), glucose (3.67 - 4.57 mmol/L), urea (8.73 - 9.67 mg/dL) and cholesterol (1.17 - 2.60 mmol/L) obtained for biochemical parameters in the control and study animals also showed significant (P<0.05) differences except for globulin. It was concluded that inclusion of Ficus sycomorus leaf meal in the diets of weaner rabbits at 5% had no adverse effect on the parameters evaluated. It was therefore, recommended that 5% inclusion level of Ficus sycomorus could be safely incorporated in the diets of weaner rabbits to meet their protein requirements as an alternative to more expensive conventional protein sources.


Author(s):  
Ashenafi Assefa Adugna ◽  
Keredin Mohammed Habib ◽  
Ojuni Odier ◽  
Tsigabu Gebresilase

Background: The presence of inadequate and inconsistent feeds to the chicken resulted in lower performance of chick production in Ethiopia. Broilers are meat type chickens with fast income generation and depend on high quality feed. Feed costs alone currently account for over 65-70% of poultry production in third world countries, there has been recent interest in determining the feeding value of different locally available alternative feeding resources. As fish is generally appreciated as one of the healthiest and cheapest source of protein and it is available in Gambella water bodies, this research is intended to investigate the effects of fish by-product inclusion in broiler feeds on growth performance. Two hundred and forty (240) day old broiler chicks (Cobb 500) were reared and assigned to four treatments for 42 days. Treatment one was the control without fish by-product meal, treatment 2 contained 2% fish by-product meal, treatment 3 contained 3% fish by-product meal and treatment 4 contained 5% fish by-product meal. Each treatment was replicated 3 times with 20 birds per replicate in a completely randomized design. Parameters measured for the experiment were the initial body weight, final weight, daily feed intake while weight gain and feed conversion ratio (FCR) were calculated. Data were analyzed using General linear model procedure of statistical analysis software (SAS) version 9.2. Results: This study indicated that the average daily feed intake of broiler significantly increased (P<0.05) in 3 and 5% fish by-product meal level to the diets during 11-20 days of age. At the age of 32 – 42 days the average body weight and daily feed intake at the age of 21-32 days were significantly (P<0.05) increased in 3 and 5% fish by-product meal level compared to control and 2% fishmeal diet. Conclusion: This study revealed that inclusion of fish by-product meal in the diet improved the performance of broiler. To utilize and exploit the potential of fish by-product meal as broiler feed in Gambella region, farmers should get training on preparation and utilization of fish by product.


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