CLINICAL AND MORPHOLOGICAL CHANGES IN GUM DURING THE MODELING OF EXPERIMENTAL GINGIVITIS

2021 ◽  
pp. 71-76
Author(s):  
N.G. Krautsova-Kukhmar ◽  
◽  
N.V. Shakavets ◽  
Т.V. Kidyasova ◽  
◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Inflammatory Process ◽  
Morphological Changes ◽  
Dosage Forms ◽  
Common Disease ◽  
Submucosal Layer ◽  
Periodontal Tissues ◽  
Experimental Gingivitis ◽  
Granular Layers ◽  
Degree Of Severity

Gingivitis is the most common disease of periodontal tissues in children. An experimental model of gingivitis is necessary to study the pathogenetic processes occurring in this disease, as well as to develop new dosage forms. Drugs based on phospholipase A are used to model gingivitis, its content in viper venom is 32—59.8 %. The aim of the work is to evaluate the clinical and morphological changes in the gum tissues in experimental gingivitis caused by the drug «Vipraxin». 20 animals were selected to simulate experimental gingivitis. They were injected once into the submucosal layer of the gum with the drug «Vipraxin», which is an aqueous solution (0,2 ml) of viper venom. All rats were biopsied for histological examination on the 6th day from the beginning of the experiment. Redness of the gingival margin, puffiness and smoothness of the contour were visually noted in all rats. Also bleeding was determined while probing. The histological preparations revealed disintegration of the cells of the spiny and granular layers of the epithelium, smoothness of the papillae of the connective tissue lamina proper, swelling and loosening of the fibrous structures, and vascular fullness. 3 groups of activity of the inflammatory process depending on the degree of severity of changes in the gum tissues: mild, moderate and severe. All clinical and morphological changes match to the inflammatory process in case of using the drug «Vipraxin». This drug can be used for modeling experimental gingivitis.

scholarly journals THE INTENSITY OF CHRONIC CATARRHAL GINGIVITIS IN CHILDREN DEPENDING ON THE AGE

2020 ◽  
Vol 73 (5) ◽  
pp. 846-849
Author(s):  
Petro A. Hasiuk ◽  
Nataliia V. Malko ◽  
Anna B. Vorobets ◽  
Viktoriya V. Ivanchyshyn ◽  
Svitlana O. Rosolovska ◽  
...  
Keyword(s):  
Inflammatory Process ◽  
Moderate Severity ◽  
Ecological Conditions ◽  
Periodontal Tissues ◽  
Time Average ◽  
High Level ◽  
Degree Of Severity

The aim: To determine the degree of severity of the inflammatory process in periodontal tissues of children living in different ecological conditions. Materials and methods: With the purpose of estimation of the condition of periodontal tissues the epidemiology examination of 642 children that live on territory with the high level of pollution and natural deficit of iodine and fluorine was conducted. Results: The study found that the increase of age of examined children causes the decrease of percentage of easy degree of severity of chronic catarrhal gingivitis, giving way to the advanced forms of the disease, and this process was more pronounced in children living in ecologically polluted region. In children-inhabitants of ecologically clean region easy degree of severity of chronic catarrhal gingivitis met more often than their peers living in environmentally polluted region. At the same time, average and heavy degree of severity of chronic catarrhal gingivitis in children living in ecologically contaminated region met more often than their peers, inhabitants of ecologically clean region. Conclusions. It was found that with increasing age of the examined children decreases the percentage of chronic catarrhal gingivitis of moderate severity.

scholarly journals Non-infectious causes of chronic pharyngitis

2018 ◽  
pp. 112-115
Author(s):  
S. Ya. Kosyakov ◽  
I. B. Angotoeva ◽  
A. N. Isamov
Keyword(s):  
Antibiotic Therapy ◽  
Sore Throat ◽  
Inflammatory Process ◽  
Common Disease ◽  
Main Symptom ◽  
Systemic Antibiotic ◽  
Pharyngeal Mucosa ◽  
Systemic Antibiotic Therapy

Chronic pharyngitis (CP) is a common disease that is not associated with infections and does not require administration of any systemic antibiotic therapy. Many non-infectious factors cause CP. Despite diversity and variability of factors, the inflammatory process of the pharyngeal mucosa lie at the root of the pathogenesis of CP. A sore throat is the main symptom of this process, which can be successfully relieved with local drugs.

scholarly journals REACTIVE OXYGEN AND NITROGEN SPECIES ROLE IN EXPERIMENTAL PERIODONTITIS DEVELOPMENT

2018 ◽  
Author(s):  
A. Ye. Demkovych
Keyword(s):  
Nitric Oxide ◽  
Lipid Peroxidation ◽  
Blood Serum ◽  
Inflammatory Process ◽  
Reactive Oxygen ◽  
Nitrogen Species ◽  
Initial Development ◽  
Periodontal Tissues ◽  
Experimental Periodontitis ◽  
Nitric Oxide Metabolites

Introduction. Activation of lipid peroxidation is one of the trigger mechanisms of periodontium injury, which is primary caused by cellular damage. Reactive oxygen and nitrogen species (RONS) are able to cause damage to a cell as well as final products of lipid peroxidation, including unsaturated aldehydes and other metabolites. Objective. The aim of the research was to determine the role of RONS and accumulation of lipid peroxidation derivatives in initial development and formation of chronical inflammatory process in periodontium. Methods. Experimental periodontitis was modeled in animals by injection of complex mixtures of microorganisms diluted in egg protein into periodontal tissues. The results of biochemical studies of free radical processes activity in blood serum were evaluated by content of diene, triene conjugates, TBA-active products and total quantity of metabolites of nitric oxide (NO2–+NO3–), which were determined on the 7th, 14th and 30th days of the experiment. Results. Generation of active forms of oxygen is more influential, providing longevity of inflammatory process. This pays attention to typical dynamics of changes in active processes of lipid peroxidation in the development and course of experimental periodontitis. The study of inflammatory process with a bacterial-immune component in the rats’ periodontal complex proved accumulation of lipid peroxidation and nitric oxide metabolites in blood serum.Conclusions. The preservation of increased lipid peroxidation and nitric oxide metabolites in blood serum of the experimental animals with acute periodontitis conduce enhance of alteration and delayed healing that result in its sequel into chronical periodontitis.

scholarly journals PECULIARITIES OF PATHOMORPHOLOGICAL DIAGNOSIS OF THE MOST COMMON RESPIRATORY INFECTIONS OF A PIG

2020 ◽  
Author(s):  
Oleksiy Tkachenko ◽  
◽  
Olena Havrylina ◽  
Keyword(s):  
Lymph Nodes ◽  
Inflammatory Process ◽  
Mononuclear Cells ◽  
Respiratory Infections ◽  
Morphological Changes ◽  
Direct Action ◽  
Immunohistochemical Analysis ◽  
Regional Lymph Nodes ◽  
Functional Changes ◽  
Progressive Development

The active spread of respiratory infections in pig farms raises the issue of differential pathomorphological diagnosis of diseases related to a single syndrome of respiratory pathologies. Pathological autopsy and histopathological examinations of organs from 72 carcasses of pigs during the fattening period were performed. Pathological autopsy of pigs was performed by complete evisceration. Histologically examined 360 lung samples with regional lymph nodes. The presence of bacterial and viral infections was confirmed by bacteriological and PCR studies. The aim of the study is to establish the characteristic differential features at the macro- and micro-level in the lungs of domestic pigs for viral and bacterial pathogens. The main tasks of the work are to determine the morphofunctional features and dynamics of pathomorphological changes in the parenchyma and immune formations of the lungs in respiratory pathology. As a result of complex pathomorphological studies of the lungs in respiratory infections of pigs found that structural and functional changes in the body have different localization, stage and nature of the pathological process, which depend on the direct action of the etiological factor. Acute catarrhal bronchopneumonia is registered in respiratory mycoplasmosis (enzootic pneumonia), which in a prolonged course turns into chronic catarrhal or catarrhal-purulent pneumonia. Hemorrhagic necrotizing pneumonia is a manifestation of actinobacillary pleuropneumonia. Interstitial (diffuse proliferative) pneumonia develops with viral pathogens - circovirus infection and reproductive and respiratory syndrome of pigs (PRRS). Serous fibrinous and fibrinous pleurisy develop in hemophilic polyserositis and actinobacillary pleuropneumonia. Pathomorphological changes of the lungs in the reproductive and respiratory syndrome of pigs are polymorphic and are manifested by the gradual progressive development of the inflammatory process from congestive hyperemia, acute catarrh to diffuse interstitial pneumonia. Pathomorphological changes of the lungs in mycoplasmosis (enzootic pleuropneumonia) of pigs are polymorphic and are manifested by the gradual progressive development of the inflammatory process, which is localized in the cranial, middle and peripheral parts of the diaphragmatic particles and is characterized by acute catarrhal bronchopneumonia. In actinobacillary pleuropneumonia pathohistological studies revealed a pronounced stage of morphological changes in the lungs and regional lymph nodes in actinobacillary pleuropneumonia. Depending on the form of the disease, serous-hemorrhagic exudation is exacerbated by fibrinogen exudation and increased migration of lymphocytes and mononuclear cells. In subacute and chronic forms of the disease, necrotic phenomena prevail in combination with areas of serous-fibrinous inflammation. In the future, further studies of immunohistochemical analysis to establish the tropism of the pathogen and the study of markers of lymphoid cells.

Simple spectrofluorimetric methods for determination of two pharmaceutically active compounds; salmeterol and trimebutine in their raw materials and pharmaceutical dosage forms in nano concentrations and testing of content uniformity

2018 ◽  
Vol 10 (37) ◽  
pp. 4511-4517
Author(s):  
M. I. Walash ◽  
Samah Abo El Abass ◽  
M. E. Fathy
Keyword(s):  
Aqueous Solution ◽  
Raw Materials ◽  
Dosage Forms ◽  
Fluorescence Properties ◽  
Content Uniformity ◽  
Pharmaceutically Active Compounds ◽  
Native Fluorescence ◽  
Active Compounds ◽  
Pharmaceutical Dosage Forms

Simple and rapid spectrofluorimetric methods based on the native fluorescence properties of salmeterol in its aqueous solution and enhanced native fluorescence of trimebutine were developed.

The application of an immunomodulator for the correction of the inflammatory process in periodontal tissues of the patients presenting with chronic generalized periodontitis

2016 ◽  
Vol 9 (3) ◽  
pp. 38 ◽  
Author(s):  
T. I. Sashkina ◽  
G. V. Poryadin ◽  
G. S. Runova ◽  
D. S. Dubrovin ◽  
D. K. Faskhutdinov ◽  
...  
Keyword(s):  
Inflammatory Process ◽  
Periodontal Tissues

scholarly journals Synaptopathy Mechanisms in ALS Caused by C9orf72 Repeat Expansion

2021 ◽  
Vol 15 ◽  
Author(s):  
Agnes L. Nishimura ◽  
Natalia Arias
Keyword(s):  
Motor Neurons ◽  
Neuronal Excitability ◽  
Morphological Changes ◽  
Glutamate Excitotoxicity ◽  
Common Disease ◽  
Neuronal Cytoskeleton ◽  
Axon Terminals ◽  
Synaptic Functions ◽  
Repeat Expansions ◽  
The Impact

Amyotrophic Lateral Sclerosis (ALS) is a complex neurodegenerative disease caused by degeneration of motor neurons (MNs). ALS pathogenic features include accumulation of misfolded proteins, glutamate excitotoxicity, mitochondrial dysfunction at distal axon terminals, and neuronal cytoskeleton changes. Synergies between loss of C9orf72 functions and gain of function by toxic effects of repeat expansions also contribute to C9orf72-mediated pathogenesis. However, the impact of haploinsufficiency of C9orf72 on neurons and in synaptic functions requires further examination. As the motor neurons degenerate, the disease symptoms will lead to neurotransmission deficiencies in the brain, spinal cord, and neuromuscular junction. Altered neuronal excitability, synaptic morphological changes, and C9orf72 protein and DPR localization at the synapses, suggest a potential involvement of C9orf72 at synapses. In this review article, we provide a conceptual framework for assessing the putative involvement of C9orf72 as a synaptopathy, and we explore the underlying and common disease mechanisms with other neurodegenerative diseases. Finally, we reflect on the major challenges of understanding C9orf72-ALS as a synaptopathy focusing on integrating mitochondrial and neuronal cytoskeleton degeneration as biomarkers and potential targets to treat ALS neurodegeneration.

scholarly journals Experimental Validation of the Use of Immobilized Form of Miramistin in the Treatment of Advanced Peritonitis

2021 ◽  
Vol 14 (1) ◽  
pp. 53-60
Author(s):  
Boris Semenovich Sukovatykh ◽  
Maria Alekseevna Zatolokina ◽  
Anastasiya Viktorovna Mosolova
Keyword(s):  
Aqueous Solution ◽  
Inflammatory Process ◽  
Comparison Group ◽  
Abdominal Cavity ◽  
Inflammatory Activity ◽  
Sodium Carboxymethylcellulose ◽  
Generalized Peritonitis ◽  
Fecal Suspension ◽  
Anti Inflammatory ◽  
Experimental Group

Introduction. Mortality in generalized peritonitis ranges from 16% to 30%.The aim of research was to experimentally study the effect of the immobilized form of 0.01% miramistin on the basis of sodium salt of carboxymethylcellulose on the course of the inflammatory process in generalized purulent peritonitis.Materials and methods. The experimental study included 288 male Wistar rats, divided into 3 groups, 96 animals each. Animals of the 1st group (control) were exposed to laparotomy and lavage of the abdominal cavity with saline under aseptic conditions 24 hours after the introduction of fecal suspension into the abdominal cavity. Simultaneously, animals of the 2nd group (comparison) underwent a thorough sanitation of the abdominal cavity with saline with removal of purulent effusion and fibrin films at the first stage, and at the second stage, they were injected 5 ml of an aqueous solution of 0.01% miramistin. In animals of the 3rd (experimental) group, 5 ml of 0.01% miramistin gel was evenly distributed over the entire surface of the peritoneum after laparotomy and sanitation of the abdominal cavity with saline solution. The anti-inflammatory activity of the dosage forms was assessed by the dynamics of leukocytosis and the leukocyte index of intoxication, and the anti-inflammatory activity was assessed by the dynamics of the number of microorganisms in the abdominal exudate. The lethality of animals in each group was estimated. The following areas were taken for histological examination: small and large intestine, parietal peritoneum, pancreas, liver.Results. The anti-inflammatory activity of the immobilized form of 0.01% miramistin on the basis of sodium carboxymethylcellulose was superior to the aqueous solution of miramistin 0.01% on the 1st day - 1.3 times, on the 3rd day - 1.6 times, on the 7th day - 1.5 times. Antimicrobial activity in animals of the experimental group was 1.3 times higher on the 1st day, 1.9 times higher on the 3rd day, and 1.7 times higher on the 7th day than in the comparison group. The mortality rate in animals of the experimental group was 1.5 times lower on the 1st day, and 1.4 times lower on the 3rd and 7th days than in animals of the comparison group. On the first day, the morphological picture of peritonitis in animals of the experimental and comparison groups had no significant differences. On the 3rd day in animals of the comparison group, the inflammatory process in the abdominal cavity was pronounced, and in the experimental group, the intensity of peritonitis began to decrease, and by the 7th day it was completely eliminated.Conclusion. The results of the study allow recommending the use of the immobilized form of 0.01% miramistin based on sodium carboxymethylcellulose in the treatment of generalized peritonitis.

scholarly journals TRIM16 Positively Regulates Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Modulating the Ubiquitination and Degradation of RUNX2

2020 ◽  
Author(s):  
Yi Zhao ◽  
Qiaoli Zhai ◽  
Hong Liu ◽  
Xun Xi ◽  
Shuai Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Alkaline Phosphatase ◽  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Periodontal Ligament ◽  
Osteogenic Potential ◽  
Common Disease ◽  
Periodontal Ligament Stem Cells ◽  
Alizarin Red ◽  
Periodontal Tissues

Abstract BackgroundPeriodontal disease is a common disease that compromises the integrity of tooth-supporting tissues. Bone regeneration is the ultimate goal of periodontal therapies, in which osteogenic differentiation of human periodontal ligament stem cells plays a critical role. The tripartite motif (TRIM)16 is downregulated in periodontal tissues of patients with periodontitis and involved in osteogenic differentiation of human bone marrow mesenchymal stem cells(hBMSCs).However, the role of TRIM16 in the osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) is largely unknown.MethodshPDLSCs were isolated and identified by immunophenotype assays using flow cytometry. Overexpression plasmids and specific short-hairpin RNAs (shRNAs) were constructed to manipulate the expression of target molecules. Alkaline phosphatase (ALP) staining, alizarin red staining (ARS) and enzyme‐linked immunosorbent assays (ELISA) were used to evaluate osteogenic potential capacity. Reverse transcription quantitative PCR (RT-qPCR) and Western blot analysis were performed to determine the expression of osteogenic-related markers and activation of relevant signaling pathways. Co-immunoprecipitation assays were performed to confirm the interactions between proteins and the ubiquitination of RUNX2. A LC-MS/MS analysis was performed to explore the different expression proteins in present of TRIM16.ResultsTRIM16 significantly promoted alkaline phosphatase activity and mineralized nodule formation, and positively regulated the osteogenic differentiation of hPDLSCs by enhancing protein expression of RUNX2, COL1A1 and OCN. Mechanistically, TRIM16 serves as a pivotal factor that stabilizes RUNX2 protein levels by decreasing CHIP-mediated K48-linked ubiquitination degradation of the RUNX2 protein. Besides, TRIM16 significantly increased expression of COL1A1 via activation of p38MAPK/RUNX2.ConclusionThis study identified a novel mechanism of TRIM16 in regulating stability of the RUNX2 protein, which may promote the osteogenic differentiation of hPDLSCs. TRIM16 may be a potential target of stem cell based-bone regeneration for periodontal therapies.

scholarly journals Avaliação macro e microscópica do trato digestório de frangos de corte alimentados com dieta contendo glicerina

2017 ◽  
Vol 8 (1) ◽  
pp. 59 ◽  
Author(s):  
Denise Cerqueira Sousa ◽  
Nadja Lamonye Alves Oliveira ◽  
Edna Teles dos Santos ◽  
Leilane Rocha Barros Dourado ◽  
Guilherme José Bolzani de Campos Ferreira
Keyword(s):  
Inflammatory Process ◽  
Morphological Changes ◽  
Lymphatic Tissue ◽  
Randomized Design ◽  
Significant Difference ◽  
Inflammatory Processes ◽  
Passive Absorption ◽  
Completely Randomized Design ◽  
Mucus Barrier ◽  
Inflammatory Infiltrates

This study aimed to evaluate the presence of morphological changes in the digestive tract of broilers fed diets containing 7% glycerol. It was used 16 animals from male Cobb 500® line, distributed in a completely randomized design in two treatments: animals fed diet without glycerin and animals fed diets containing 7% glycerol. It was observed that the addition of glycerin at 7% in the diet of broilers promotes the occurrence of significant inflammatory infiltrates in the proventriculus, caecum and colon of such animals, macroscopically the addition of glycerin in the diet did not cause any significant difference, but these differences had no significant effect on zootechnical development of such animals. It is possible to conclude that the use of glycerin in the diet can cause localized inflammatory processes, due to its alcoholic feature that degrades the protective mucus barrier and it presents passive absorption and exposes the mucus epithelium to the gastric juice action, thus promoting the mucosa inflammatory process. In the large intestine (cecum and colon) the inflammatory response showed greater intensity as a function of these organs have higher lymphatic tissue (nodular and diffuse)concentration and at this portion the intestine performs the fluids reabsorption directly competing with glycerin which is highly hygroscopic.

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