Omega-3 and Vitamin E Supplementation Effect to Rattus Norvegicus Placental Apoptosis Marker: Pre-Eclampsia Model

Background: Pre-eclampsia has been associated with the increased of placental apoptosis caused by oxidative stress. Omega-3 fatty acids and vitamin E had beneficial function to maintain cell membranes, prevent oxidative stress and inhibit the production of proinflammatory cytokines. The purpose of the study was to determine the effect of omega-3 and vitamin E supplement to BCL-2 and BAX on PE rats model. Methods: This research has been worked out at animal house and Biomedical Laboratory of the Medical Faculty Andalas University. The design of this research was experimental study with post test only control group design. There were 35 pregnant rats were divided into 5 groups. Group K- without treatment, group K+ was given L-NAME, group P1 was given L-NAME + omega-3, group P2 was given L-NAME + vitamin E and group P3 was given L-NAME + omega-3 + vitamin E. The L-NAME dose was 50mg/kg/day, At the 19th day of gestation, the pregnancy was terminated and the placental tissue was collected for examining BCL-2 and BAX levels by using ELISA kit. The data were analyzed by one way ANOVA and post hoc test LSD. Results: The mean levels of BCL-2 in each group were K- =62.49 ng/mg, K+ =50.64 ng/mg, P1 =58.79 ng/mg, P2 =53.70 ng/mg and P3 =70.10 ng/mg. The mean levels of BAX were K- =72.06 ng/mg, K+ =78.61 ng/mg, P1 =73.71 ng/mg, P2 =74.31 ng/mg and P3 =71.63 ng/mg. The analysis results concluded that there were significant differences between omega-3 and vitamin E supplements on BCL-2 levels (p=0.000) and BAX levels (p=0.001). Conclusion: Omega-3 (DHA 120 mg, EPA 180 mg) and vitamin E (α-tocopherol 300 iu) suplement increased BCL-2 level and decreased BAX on pre-eclampsia rats model. Keywords: Omega-3, Vitamin E, BCL-2, BAX, Pre-eclampsia.

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Pengaruh pemberian vitamin E terhadap kadar neutrofil setelah latihan fisik

Jurnal e-Biomedik ◽

10.35790/ebm.4.2.2016.14343 ◽

2016 ◽

Vol 4 (2) ◽

Author(s):

Kristo Warong ◽

Damajanty H.C. Pangemanan ◽

Joice N.A. Engka

Keyword(s):

Oxidative Stress ◽

Vitamin E ◽

Physical Exercise ◽

Tissue Injury ◽

The Body ◽

Control Group ◽

Control Group Design ◽

Group Design ◽

Post Test ◽

Test Control

Absract: Physical exercise can improve and maintain physical fitness. However, physical exercise can lead to oxidative stress that can reduce the activity of antioxidants. Vitamin E is a fat-soluble antioxidant that could free radicals in the body. Neutrophils play an active role in the process of phagocytosis of bacteria and other microorganisms the damaged tissue caused by tissue injury. This study was aimed to obtain the effect of vitamin E on neutrophil count after physical exercise. This was a field experimental study with a pre post test control group design. Data were analyzed with the Mann Whitney U (α = 0.05). The physical exercise was playing futsal for 60 minutes. Respondents were 30 male respondents divided into 2 groups: treatment and control groups. The levels of neutrophils were examined after physical exercise and after the administration of vitamin E 400 IU for seven days. Data analysis of the effect of vitamin E on neutrophil level after physical exercise showed a p value of 0.031. Conclusion: Vitamin E influenced the levels of neutrophils after physical exercise.Keywords: physical exercise, oxidative stress, vitamin E, neutrophil Abstrak: Latihan fisik merupakan aktivitas yang dilakukan seseorang untuk meningkatkan atau memelihara kebugaran tubuh. Latihan fisik dapat menimbulkan stres oksidatif sehingga dapat menurunkan aktivitas antioksidan. Vitamin E merupakan antioksidan yang larut dalam lemak dan berfungsi untuk mengurangi radikal bebas yang terdapat dalam tubuh. Neutrofil berperan aktif dalam proses fagositosis bakteri, mikroorganisme, dan membersihkan sisa jaringan rusak yang disebabkan oleh cedera jaringan. Penelitian ini bertujuan untuk mengetahui pengaruh vitamin E terhadap kadar neutrofil setelah latihan fisik. Jenis penelitian ialah eksperimental lapangan dengan pre post test control group design. Untuk menguji signifikansi penelitian digunakan uji Mann Whitney U (α = 0,05). Latihan fisik berupa olahraga futsal selama 60 menit dilakukan oleh 30 responden laki-laki yang dibagi dalam 2 kelompok yaitu perlakuan dan kontrol. Kadar neutrofil diukur setelah latihan fisik dan setelah pemberian vitamin E 400 IU selama 7 hari. Hasil statistik menunjukkan terdapat pengaruh bermakna dari vitamin E pada kelompok perlakuan (p=0,031). Simpulan: Terdapat pengaruh vitamin E terhadap kadar neutrofil setelah latihan fisik. Kata kunci: latihan fisik, stres oksidatif, vitamin E, neutrofil

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The Effect of Administration of Omega-3 and Vitamin E on the Levels of Reactive Oxygen Species (ROS) and Nitric Oxide (NO) in Preeclampsia White Rats (Rattus Norvegicus)

International Journal of Research and Review ◽

10.52403/ijrr.20211202 ◽

2021 ◽

Vol 8 (12) ◽

pp. 7-15

Author(s):

Dina Taufia ◽

Syamel Muhammad ◽

Arni Amir

Keyword(s):

Oxidative Stress ◽

Fatty Acids ◽

Vitamin E ◽

Control Group ◽

Omega 3 Fatty Acids ◽

Omega 3 ◽

Pregnant Rats ◽

White Rats ◽

Significant Difference ◽

One Way Anova

Background: Preeclampsia is a disease with a variety of theories that describe the uncertainty of the pathophysiology. According to the oxidative stress theory, preeclampsia originates from the failure of trophoblast invasion during the implantation process, causing ischemia and placental hypoxia, which in turn causes cell damage, including placental endothelial cell dysfunction. Omega-3 fatty acids and vitamin E have an important role in preventing preeclampsia. Omega-3 fatty acids play an important role in maintaining cell membranes and anti-inflammatory processes. At the same time, vitamin E acts as a fat-soluble antioxidant that can prevent oxidative stress, inhibit proinflammatory cytokines, and protect fatty acids from oxidation. Aim: The purpose of this study was to determine the effect of omega-3 and vitamin E on the level of ROS and NO in pregnant rats with preeclampsia models. Method & Material: This type of research is experimental with Post-Test Only Control Group Design. The sample consisted of 35 pregnant rats, which were divided into five groups. On the 19th day, blood serum was taken to check the levels of ROS and NO. The measuring instrument used is a spectrophotometer with the ELISA method. Data were analyzed using the Shapiro Wilks normality test. After the parametric test was completed, the hypothesis was tested using one-way ANOVA. Results: The average levels of ROS in each group were K- : 121,684 ng/L, K+ : 143,885 ng/L, P1 : 136,250 ng/L, P2 : 132.433 ng/L, and P3 : 122,993 ng/L. The average levels of NO obtained were K-: 29,502 ng/L, K+: 26,053 ng/L, P1: 27,250 ng/L, P2: 27,555 ng/L, and P3: 32,278 ng/L. The results of one-way ANOVA analysis showed that the administration of omega-3 and vitamin E had a significant difference between the control and treatment groups, both at levels of ROS (p=0.001) and levels of NO (p=0.001). Conclusion: The administration of omega-3, vitamin E, and omega-3 plus vitamin E can reduce ROS levels in pregnant rats with preeclampsia models. There is an increase of NO levels only in the administration of omega-3 plus vitamin E. Keywords: [Omega-3, Vitamin E, Oxidative stress, ROS, NO, Preeclampsia].

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The Effect of Anonymous Peer Assessment on Writing Ability of the Eleventh Grade Students at SMK PGRI 1 Singaraja

Journal of Psychology and Instructions ◽

10.23887/jpai.v2i1.13735 ◽

2018 ◽

Vol 2 (1) ◽

pp. 1

Author(s):

A.P. Adhiyanti ◽

A.A.I.N Marhaeni ◽

I.P.I Kusuma

Keyword(s):

Peer Assessment ◽

Lesson Plan ◽

Control Group ◽

Control Group Design ◽

Writing Ability ◽

Group Design ◽

Writing Test ◽

Post Test ◽

The Mean ◽

Academic Year

This study aimed to investigate the effect of anonymous peer assessment on students’ writing ability. Quantitative approach was used in this study. Post-test only control group design was the design used in this experimental study. The post-test only control group design was designed to discover the purpose of this study which was to investigate the significant effect on writing ability between students who obtain feedback from anonymous peer assessment and students who obtain feedback from conventional assessment. The study was conducted at SMK PGRI 1 Singaraja. The eleventh grade students in Academic Year 2017/2018 were chosen as the population. The samples were 44 students from XI AP and XI UPW. The instruments used in this study were lesson plan, anonymous peer checklist, writing scoring rubric, and writing test. Writing scoring rubric and writing test were used to obtain the data through post-test while lesson plan and anonymous peer assessment were used during the treatment. The data then was analyzed by using SPSS 16. The results of the analysis show that (1) the mean score of the experimental group was 77.55 while the mean score of the control group was 68.77. (2) The t-observe was 3.892 and it exceeded the t-critical value which was 2.018. Therefore, based on the aforementioned results, it can be concluded that there was a significant difference on writing ability between the eleventh grade students who obtained feedback from anonymous peer assessment and those who obtained feedback from conventional assessment at SMK PGRI 1 Singaraja in academic year 2017/2018.

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The Effect of Propanolol on C-Reactive Protein in Patients with Severe Burns at Dr Soetomo Hospital Surabaya

Jurnal Rekonstruksi dan Estetik ◽

10.20473/jre.v4i1.24352 ◽

2021 ◽

Vol 4 (1) ◽

pp. 27

Author(s):

Muhammad Aulia U H ◽

Iswinarno Doso Saputro ◽

Magda Rosalina Hutagalung

Keyword(s):

Treatment Group ◽

Albumin Level ◽

Mean Value ◽

The Body ◽

Control Group ◽

Control Group Design ◽

Severe Burns ◽

Hypermetabolic State ◽

Post Test ◽

The Mean

Background: The incidence of burns in Indonesia progressively increases with the increase in its population and industries. From January to September 2000, 158 patients were treated in the burn unit of Dr Soetomo Hospital with a mortality rate reaching 5,8%. Burns have a direct effect in causing both local and systemic changes in the body, not occurring in other injuries. In severe burns, a hypermetabolic state can occur, which increases cardiac workload and causes muscle atrophy and other morbidities. The purpose of this study is to examine the effect of propranolol on the hypermetabolic state in severely burned patients by measuring various clinical & laboratory parameters.Methods : This is an experimental study using pre and post test control group design with the objective of assessing the treatment outcome with oral propranolol given in 15 consecutive days for burn patients involving 25%- 60% TBSA. Measurements were taken three times, on day 0, 7 and 14.Results : Obtained 16 samples divided into 2 groups. In the treatment group, there was a significant decrease in CRP levels on days 0, 7 and 14 (p <0.05). The Mid Arm Circumference variable did not obtain a significant decreasing on days 0, 7 and 14. The albumin level studied showed a significant decreasing on day 0 & 7 days with a value of p = 0.045. From the comparison between the two groups, there were significant differences in CRP levels on days 0 & 14, with the mean value of the treatment group -5.12 + 2.88 and the mean value of the control group 2.86 + 7.37, and the value of p = 0.019.Conclusions: This study successfully proved that the administration of propranolol can overcome the effects of hypermetabolism which is characterized by decreasing in CRP levels.

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Pemberian Ekstrak Floret Pisang Raja (Musa x paradisiaca) Mencegah Penurunan Kadar Superoksida Dismutase (SOD) pada Hati Mencit (Mus musculus) BALB/c dengan Aktivitas Fisik Berlebih

JURNAL BIOMEDIK (JBM) ◽

10.35790/jbm.9.3.2017.17338 ◽

2017 ◽

Vol 9 (3) ◽

Author(s):

Okky Irtanto ◽

Alex Pangkahila ◽

IGM Aman

Keyword(s):

Oxidative Stress ◽

Superoxide Dismutase ◽

Mus Musculus ◽

Liver Tissue ◽

Day Treatment ◽

Control Group ◽

P Value ◽

Control Group Design ◽

Group Design ◽

Post Test

Abstract: Overtraining accelerates aging due to the excessive production of free radicals that can cause oxidative stress. Banana floret extract contains bioactive compounds with antioxidant capacity which can increase the body's defence to deal with the oxidative stress by increasing the level of superoxide dismutase (SOD). This study was aimed to prove that the banana (Musa x paradisiaca) floret extract could prevent the decrease of superoxide dismutase (SOD) levels in overtraining-induced mice (Mus musculus) BALB/c liver. This was a true experimental study with the post-test only control group design. Subjects were 36 male mice (Mus musculus), BALB/c strain, 12 weeks old, weighing 20-22 g, which were divided into two groups with 18 mice each. The control group (P0) was treated with a placebo of 1 ml aquadest and overtrained for 14 days meanwhile the treatment group (P1) was treated with banana (Musa x paradisiaca) floret extract of 400 mg/kgBW/day and overtrained for 14 days. The results showed that after 14-day treatment, the mean SOD level in the liver tissue of the P0 group was 568.82±9.558 U/mg protein whereas in the P1 group was 588.37±10.629 U/mg protein (P < 0.01). The t-independent test showed a t value of -5.804 and a P value of 0.000 which indicated that after treatment, the levels of SOD in liver tissue of both groups were significantly different. Conclusion: Banana (Musa x paradisiaca) floret extract could prevent the decrease of superoxide dismutase (SOD) levels in the liver tissue of overtraining-induced mice (Mus musculus) BALB/c.Keywords: banana floret, SOD, liver, overtrainingAbstrak: Aktivitas fisik berlebih mempercepat penuaan karena meningkatkan produksi radikal bebas yang dapat menyebabkan stres oksidatif. Ekstrak floret pisang mengandung senyawa bioaktif dengan kapasitas antioksidan yang dapat meningkatkan pertahanan tubuh dalam menghadapi stres oksidatif melalui peningkatan kadar superoksida dismutase (SOD). Penelitian ini bertujuan untuk membuktikan bahwa pemberian ekstrak floret pisang raja (Musa x paradisiaca) dapat mencegah penurunan kadar SOD pada hati mencit (Mus musculus) BALB/c dengan aktivitas fisik berlebih. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Subjek penelitian ialah 36 ekor mencit (Mus Musculus) BALB/c, jantan, berumur 12 minggu, berat badan 20-22 gr, yang dibagi menjadi dua kelompok masing-masing berjumlah 18 ekor mencit. Kelompok kontrol (P0) diberikan plasebo berupa aquadest sebanyak 1 ml dengan aktivitas fisik berlebih selama 14 hari, dan kelompok perlakuan (P1) diberikan ekstrak floret pisang raja (Musa x paradisiaca) dosis 400 mg/kgBB mencit per hari dicampur aquadest hingga 1 ml dengan aktivitas fisik berlebih selama 14 hari. Hasil penelitian menunjukkan rerata kadar SOD jaringan hati pada kelompok kontrol (P0) sesudah perlakuan (post-test) ialah 568,82±9,558 U/mg protein, sedangkan pada kelompok perlakuan (P1) ialah 588,37± 10,629 U/mg protein. Analisis kemaknaan dengan T-Independent mendapatkan nilai t= -5,804 dan nilai P = 0,000 yang menunjukkan bahwa sesudah perlakuan (post-test), kadar SOD jaringan hati pada kedua kelompok berbeda sangat bermakna. Simpulan: Ekstrak floret pisang raja (Musa x paradisiaca) dapat mencegah penurunan kadar SOD pada hati mencit (Mus musculus) BALB/c dengan aktivitas fisik berlebih.Kata kunci: floret pisang raja, SOD, hati, aktivitas fisik berlebih

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Effects of Propolis Extract Supplementation during Pregnancy on Stress Oxidative and Pregnancy Outcome: Levels of Malondialdehyde, 8-Oxo-2′-Deoxogunosine, Maternal Body Weight, and Number of Fetuses

Jurnal Kedokteran Brawijaya ◽

10.21776/ub.jkb.2021.031.03.5 ◽

2021 ◽

Vol 31 (3) ◽

pp. 156

Author(s):

Joko Wahyu Wibowo ◽

Minidian Fasitasari ◽

Siti Thomas Zulaikhah

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Pregnancy Outcomes ◽

Feeding Tube ◽

Control Group ◽

Maternal Body ◽

Control Group Design ◽

Pregnant Rats ◽

Propolis Extract ◽

Group I

Oxidative stress is related to pregnancy complications that could increase maternal and infant mortality. This study aimed to determine the effect of propolis extract supplementation during pregnancy on oxidative stress level and pregnancy outcomes utilizing Malonedealdehyde (MDA) and 8-Oxo-2′-Deoxogunosine (8-OHdG) levels, maternal body weight, and the average number of fetuses as the parameters. The study was conducted by using a posttest only control group design on 24 pregnant Wistar rats, which were divided into four groups. Group I was control, Group II-IV were the treatment groups given propolis extract of 1.8mg, 3.6mg, and 7.2mg/200gBW/day, respectively. The standard feed given was AIN93G dose of 20g/day and distilled water ad libitum. Propolis extract was given using a gastric feeding tube every morning for 20 days. At the end of the treatment, body weight was meisured and blood collected for assessed MDA and 8-OHdG levels by ELISA method and then we performed abdominal surgery to count number of fetuses. The result are there were decreasing level of MDA and 8-OHDG by administration of propolis significantly (p<0.05) group: I: 2,04±0,091, II: 1,55±0,067, III: 1,05±0,176, IV: 0,73±0,075 (mmol/mL) (p=0.001); 8 OHdG level (ng/mL) group I: 10,02±0,403, II: 8,60±0,078, III: 7,89±0,051, IV: 7,53±0,063 (p=0,001). Average of maternal body weight (g) were increased: group I: 228,33±3,93, II: 237,17±4,36, III: 244,83±4,02, IV: 248,00±5,76 (p=0,001) and Average number of fetuses tend to increased as well, group I : 8,5±0,05, II: 7,8±0,41, III: 9,5±1,05, IV: 9,6±0,52 (p=0,02). The conclusion of this research are supplementation of propolis extract in pregnant rats can reduce oxidative stress and improve pregnancy outcomes.

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The Effect of Using Word Family Game on The Vocabulary Mastery of The Fifth Grade Students at SD Negeri 1 Astina in Academic Years of 2017/2018

Journal of Psychology and Instructions ◽

10.23887/jpai.v1i3.12573 ◽

2017 ◽

Vol 1 (3) ◽

pp. 122

Author(s):

Ni Komang Amelia Kartadi

Keyword(s):

Fifth Grade ◽

Control Group ◽

Control Group Design ◽

Fifth Grade Students ◽

Conventional Teaching ◽

Post Test ◽

The Mean ◽

And Control ◽

Experimental Group ◽

Better Than

This study aimed at investigating of the effect of using word family game committed by the fifth grade student in SD Negeri 1 Astina in mastering the English vocabulary. This study was True-Experimental with post-test only control group design. The population was 59 students of the fifth grade and the sample was selected by using cluster random sampling. The sample of this research study was 22 students in 5A as an Experimental Group while 22 students in 5B as a Control Group. The experimental group was taught by using Word Family Game and control group was taught without using Word Family Game. The data was analyzed descriptively and inferentially through SPSS 16.0 Program. Descriptively, the students in experimental group were achieved better than the students in control group. It was proven by the result of the mean score of the experimental group was 90.45, while the mean score of control group was 83.18. The result of the t-test also showed that the score of the (tobs) was 3.393 which the score of (tcv) was 1.682 which based on the degree of freedom was 42. It showed that the (tobs) > (tcv), where: 3.393 > 1.682. It could be concluded that the word family game had a significant effect on the students’ vocabulary mastery rather than using conventional teaching.

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Pengaruh Model SFAE Berorientasi Tri Hita Karana Terhadap Hasil Belajar IPA

Jurnal Ilmiah Sekolah Dasar ◽

10.23887/jisd.v3i2.17773 ◽

2019 ◽

Vol 3 (2) ◽

pp. 202

Author(s):

I G. N. Pt. Adi Laksana Putra

Keyword(s):

Primary Schools ◽

Science Achievement ◽

Fifth Graders ◽

Control Group ◽

Control Group Design ◽

Significant Difference ◽

Post Test ◽

The Mean ◽

Academic Year ◽

Experimental Group

This study aimed at investigating the effect of Student Facilitator and Explaining (SFAE) technique based on Tri Hita Karana towards students’ science achievement in fifth grade of Buleleng district primary schools cluster XV academic year 2017/2018. This study was an experimental study utilizing non-equivalent post-test only control group design. The population of this study was fifth graders of Buleleng district primary schools cluster XV and the sample of this study was fifth graders of SD Negeri 2 Kalibukbuk as the experimental group and fifth graders of SD Negeri 3 Anturan as the control group. The data collection was utilizing instrument in the form objective test. Then, the obtained data was analysis using both descriptive and inferential (t-test) statistical analysis. The result showed that there was a difference of mean score between both groups. The mean score for experimental group was 23,12 while the mean score for control group was 16,21. Furthermore, hypothesis testing showed that tobs>tcv (tobs=6,821 > tcv=1,99962). Based on the result of the study, it can be concluded that there was a significant difference on students’ science achievement between the students taught using Student Facilitator and Explaining (SFAE) technique based on Tri Hita Karana and the students taught without Student Facilitator and Explaining (SFAE) technique based on Tri Hita Karana at fifth graders of Buleleng district primary schools cluster XV academic year 2017/2018

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PENGARUH PEMBERIAN MINYAK IKAN LEMURU (Sardinella Longiceps) SECARA SISTEMIK TERHADAP JUMLAH OSTEOBLAS PADA PROSES PENYEMBUHAN SOKET GIGI

DENTA ◽

10.30649/denta.v13i1.181 ◽

2019 ◽

Vol 13 (1) ◽

Author(s):

Eddy Hermanto ◽

Soemartono Soemartono ◽

Dwi Surya Sudrajat

Keyword(s):

Wound Healing ◽

Fish Oil ◽

Tooth Extraction ◽

Control Group ◽

Omega 3 ◽

Control Group Design ◽

Tooth Socket ◽

Post Test ◽

Socket Healing ◽

Sardinella Longiceps

<pre>Background: Sockets healing is a dynamic, continuous and overlapping process. Osteoblasts are important cells in healing sockets that will mineralize into bone until the injured tissue heals. Lemuru fish oil (Sardinella longiceps) contains omega 3 which can increase the number of osteoblasts so that it accelerates the process of socket healing after tooth extraction. Objective: To determine the effect lemuru fish oil that given systemically in increasing the number of osteoblasts in post-extraction tooth socket wound healing. Materials and methods: Experimental research laboratories with post test only control group design. Samples of 33 male Wistar mice were divided randomly into 3 groups. Group K as a control group that was not given lemuru fish oil, group P1 as a group given lemuru fish oil 1.5ml / 200g BB, and P2 group as a group given 1 ml / 200g BB lemuru fish oil. Extraction of left mandibular first incisor was carried out, then extract was given once a day for 7 days with gastric sonde. Observations were made on the 14th day after tooth extraction by calculating the number of osteoblasts in the histology sample using HE (Hematoxylin Eosin). Results: In each treatment group there were significant differences (P <0.05) and significant comparisons (P> 0, 05). Conclusion: Giving lemuru fish oil can increase the number of osteoblasts to post-extraction tooth socket wound healing.</pre>

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Gebe Ratlara uygulanan Akrilamid ve E Vitaminin Plasenta Dokusu Üzerine Olası Etkilerinin Araştırılması

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v5i4.349-352.1084 ◽

2017 ◽

Vol 5 (4) ◽

pp. 349

Author(s):

Mehmet Erman Erdemli ◽

Eyüp Altınöz ◽

Zeynep Aksungur ◽

Zümrüt Doğan ◽

Harika Gözükara Bağ ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin E ◽

Total Antioxidant Capacity ◽

Corn Oil ◽

Reduced Glutathione ◽

Control Group ◽

Vaginal Smear ◽

Protective Agent ◽

Pregnant Rats ◽

Total Antioxidant

Investigate the changes that occur in the placenta tissues of pregnant rats that were administered acrylamide (AA) and vitamin E as a protective agent during pregnancy. Thirty rats that were proven positive for pregnancy with vaginal smear test were randomly distributed into control, corn oil, vitamin E, acrylamide and vitamin E + acrylamide groups. Pregnant rats were decapitated on the 20th day of the experiment. Malondialdehyde (MDA), reduced glutathione (GSH), total antioxidant capacity (TAS), total oxidant capacity (TOS) and Xanthine oxidase (XO) levels were measured in placenta tissues. It was determined that acrylamide application during pregnancy statistically significantly increased MDA, TOS and XO levels and reduced GSH and TAS levels in the placenta tissue of pregnant rats when compared to all other groups, and GAS and TAS levels statistically significantly increased in vitamin E administered group when compared to all other groups and TOS and XO levels were decreased to control group levels. It was observed that orally administered AA changed the antioxidant / oxidant equilibrium favoring the oxidants by increasing MDA, XO and TOS levels in pregnant rats and caused oxidative stress, while vitamin E administration returned the antioxidant / oxidant equilibrium back to normal levels, preventing oxidative stress induced toxicity.

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