scholarly journals PHYTOCHEMICAL ANALYSIS, ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF THYMUS VULGARIS L.

2020 ◽  
Vol 1 (2) ◽  
Author(s):  
Moaiza Iftikhar ◽  
Salma Batool
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Biologically Active ◽  
Thymus Vulgaris ◽  
Plant Sample ◽  
Phytochemical Analysis ◽  
Bacterial Strains ◽  
Anti Oxidant ◽  
Dpph Scavenging ◽  
Dpph Scavenging Assay

Medicinal activities of Thymus vulgaris L. were studied in the underlying research which was conducted in University of Central Punjab, Lahore. The powdered plant sample was collected and subjected to extraction and filtration by dissolving it in seven different solvents and thus the fractions obtained, were used for further analysis. The qualitative and quantitative analysis was carried out which revealed that this plant contained many biologically active compounds like saponin, alkaloids etc. These compounds have enormous bioactive roles. Antimicrobial activity was also determined in which four bacterial strains were used and the susceptibility of all the fractions of plant extract was determined by their inhibition zones which were calculated in mm. The results showed that water and chloroform extracts of plant has highest activity. The antioxidant activity was also determined by DPPH scavenging assay for which different concentrations of all plant extracts were made and their absorbance was noted to measure the percentage inhibition of each concentration, which revealed that this plant has significant antioxidant activity. The results have been represented using statistical approach that revealed that T. vulgaris plant has significant antibacterial and anti-oxidant activities and phytochemical analysis also proved that it has many useful compounds which have voluminous biological activity and these could be used in the formation of novel drugs.

Comprehensive GC-MS and FT-IR Profiling of Coleus zeylanicus essential oil and investigation of its anti-fungal and anti - oxidant activities

2021 ◽  
Vol 12 (1) ◽  
pp. 636-642
Author(s):  
Lakshmi M ◽  
Nandagopal S
Keyword(s):  
Antioxidant Activity ◽  
Candida Albicans ◽  
Essential Oil ◽  
Malassezia Furfur ◽  
Fungal Strains ◽  
Anti Oxidant ◽  
Ft Ir ◽  
Dpph Scavenging ◽  
Dpph Scavenging Assay ◽  
Anti Fungal

To evaluate the leaf volatile constituents of essential oil of Coleus zeylanicus and evaluate their anti-oxidant and anti-fungal activity. The Chemical composition of Coleus zeylanicus essential oil was determined using GC-MS and FT-IR analytical techniques. The antioxidant activity was evaluated using DPPH scavenging assay. The anti-fungal effect was tested against two potential pathogenic fungal strains - Candida albicans and Malassezia furfur using agar well diffusion method. The essential oil was profiled by the presence of sesquiterpene hydrocarbons 90.67% of their total composition followed by oxygenated monoterpenes and monoterpene hydrocarbons as 5.3% and 2.1% respectively. The GC-MS results showed 14 compounds from Coleus zeylanicus leaf EO representing 98.07% of the total oil composition. The major component was identified as a-Gurjunene (35.94%), a-bisabolol (10.82%) and G-selinene (4.26%). EO showed remarkable antioxidant activity values of IC50 = 59.78± 3.21µg/ml by DPPH scavenging assay. The essential oil showed interesting anti-fungal effects against two pathogenic fungal strains. The most sensible strains to Coleus zeylanicus EO was Malassezia furfur (32.00±0.50mm) compared to that of Candida albicans (15.00±1.25mm). Hence, Coleus zeylanicus EO has potential application against fungal infection and oxidative stress-related diseases. However, further investigations are necessary to isolate and investigate the action mechanism of these bioactive compounds.

scholarly journals Phytochemical Analysis, Pharmacological and Safety Evaluations of Halophytic Plant, Salsola cyclophylla

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2384
Author(s):  
Hamdoon A. Mohammed ◽  
Mohsen S. Al-Omar ◽  
Salman A. A. Mohammed ◽  
Ahmad H. Alhowail ◽  
Hussein M. Eldeeb ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
High Concentration ◽  
Halophytic Plant ◽  
Anti Oxidant ◽  
Aqueous Ethanolic Extract ◽  
Phytochemical Constituents ◽  
Anti Inflammatory ◽  
Dpph Scavenging ◽  
Anti Inflammation

Salsola cyclophylla, an edible halophyte, is traditionally used for inflammation and pain. To confirm the claimed anti-inflammatory and analgesic properties, a detailed study on respective pharmacological actions was undertaken. The activities are contemplated to arise from its phytoconstituents. The LC-MS analysis of S. cyclophylla 95% aqueous-ethanolic extract revealed the presence of 52 compounds belonging to phenols, flavonoids, coumarins, and aliphatics class. A high concentration of Mn, Fe, and Zn was detected by atomic absorption spectroscopic analysis. The ethyl acetate extract showed the highest flavonoid contents (5.94 ± 0.04 mg/g, Quercetin Equivalents) and Fe2+-chelation (52%) potential with DPPH radicals-quenching IC50 at 1.35 ± 0.16 mg/mL, while the aqueous ethanolic extract exhibited maximum phenolics contents (136.08 ± 0.12 mg/g, gallic acid equivalents) with DPPH scavenging potential at IC50 0.615 ± 0.06 mg/mL. Aqueous ethanolic extract and standard quercetin DPPH radicals scavenging’s were equal potent at 10 mg/mL concentrations. The aqueous ethanolic extract showed highest analgesic effect with pain reduction rates 89.86% (p = 0.03), 87.50% (p < 0.01), and 99.66% (p = 0.0004) after 60, 90, and 120 min, respectively. Additionally, aqueous ethanolic extract exhibited the highest anti-inflammation capacity at 41.07% (p < 0.0001), 34.51% (p < 0.0001), and 24.82% (p < 0.0001) after 2, 3, and 6 h of extract’s administration, respectively. The phytochemical constituents, significant anti-oxidant potential, remarkable analgesic, and anti-inflammatory bioactivities of extracts supported the traditionally claimed anti-inflammatory and analgesic plant activities.

scholarly journals Ultrasonically-Extracted Marine Polysaccharides as Potential Green Antioxidant Alternatives

Proceedings ◽  
2020 ◽  
Vol 67 (1) ◽  
pp. 23
Author(s):  
Hanaa L. Essa ◽  
Hania A. Guirguis ◽  
Mayyada M. H. El-Sayed ◽  
Dalia Rifaat ◽  
Mohamed S. Abdelfattah
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Algal Species ◽  
Avicennia Marina ◽  
Ulva Lactuca ◽  
Biologically Active ◽  
Sulfated Polysaccharides ◽  
Total Phenolic ◽  
Sugar Substitutes ◽  
Pharmaceutical Industries

Marine-extracted sulfated polysaccharides (SPs) have been the subject of myriad research since they are considered an eco-friendly source of biologically active compounds. Meanwhile, food and pharmaceutical industries are urgently producing natural sugar substitutes and antioxidants as alternatives to synthetic ones which are associated with cytotoxicity and safety issues. This study assesses the potential of using marine SPs obtained via the ultrasonic-assisted extraction of different marine species, to utilize them as antioxidant sugar substitutes. The carbohydrate, total phenolic contents and antioxidant activities were measured for SP extracts of the algal species of Ulva lactuca, Jania rubens and the marine plant mangrove Avicennia marina. These SPs were structurally elucidated by Fourier Transform Infrared (FTIR) spectroscopic and high-performance liquid chromatography (HPLC) analyses. The results revealed that SPs’ highest yield percent was obtained from Ulva lactuca, 5.50 ± 0.25%. The SPs of Avicennia marina had the highest carbohydrate content, 44 ± 1% and antioxidant activity, 78.85 ± 0.06 at the 100 μg/mL concentration and 89.50 ± 0.21 at the 250 μg/mL concentration. Meanwhile, the highest phenolic content was exhibited by algal SPs obtained from Jania rubens, 132.60 ± 2.50 mgGa/g. Results also showed that all extracts have potent antioxidant activity, while the highest antioxidant activity belonged to the SPs of Avicennia marina owing possibly to their balanced glucose and galactose contents as measured by HPLC. This work emphasizes the need to consider sulfated polysaccharides from marine sources for their antioxidant activity and to correlate it with their monosaccharide content to determine the effect of reducing sugar concentration on the antioxidant activity.

scholarly journals PRELIMINARY SCREENING OF PHYTOCHEMICAL CONSTITUENTS, ANTIOXIDANT, AND ANTI-BACTERIAL ACTIVITIES IN THE METHANOLIC LEAF EXTRACT OF MELIOSMA SIMPLICIFOLIA (L.)

2021 ◽  
pp. 100-104
Author(s):  
PAVITHRA S ◽  
SEKAR T
Keyword(s):  
Leaf Extract ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Hot Water ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Dpph Scavenging

Objective: In the present study is investigated of phytochemicals and antioxidant activities of the leaf extracts from Meliosma simplicifolia (L.). Methods: The seaweed sample was subjected to extraction with solvents of different polarities (hot water, methanol, acetone, chloroform, and petroleum ether) and screened for phytochemicals according to standard methods. The ability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals was determined by in vitro antioxidant assays using 2,2-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, reducing power assay, and superoxide radical (O2•) scavenging activity. The ICPMS and GC-MS analysis of the methanol leaf extract of M. simplicifolia was revealed the presence of antibacterial. Results: Phytochemical analysis revealed the presence of Preliminary metabolites Quantitative studies of estimated phenol, flavonoid and tannin, as for the methanol extract of stem showed the highest content of phenolic compounds (40.71±0.94 GAE mg/100). Antioxidant activities were concluded the estimation M. simplicifolia leaf for as followed the studies. In leaf the highest DPPH scavenging activity (132.3 μg/ml), ICP-MS analysis of the leaf extract showed the presence minerals such as Mg, Fe, Cu, Na, and Zn in excess. The leaf extract of the plant was also tested for its antibacterial activity and results confirmed that it has potential activity. Conclusion: The preliminary studies in the methanol extract of the leaf of M. simplicifolia are suggestive of the antibacterial potentials of the methanol extract of leaves of M. simplicifolia.

In vitro antioxidant activity and phytochemical analyses of Acalypha godseffiana (Euphobiaceae) leaf extracts

2021 ◽  
Vol 25 (1) ◽  
pp. 75-79
Author(s):  
S.O. Olubodun ◽  
G.E. Eriyamremu ◽  
M.E. Ayevbuomwan ◽  
C.I. Nzoputa
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antioxidant Vitamin ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Anti Oxidant ◽  
In Vitro Antioxidant Activity

The presence of various bioactive components makes it necessary to analyse plants for their potential to act as a source of useful treatments and cures for many inflammatory, infectious and pathogenic diseases. This study was carried out to determine phytochemicals and in-vitro antioxidant activities of the leaf extracts of Acalypha godseffiana. The leaves of A. godseffiana were collected, dried, pulverized and extracted separately with methanol and water using maceration method. The extract was concentrated in vacuo with rotary evaporator at 40oC. The extracts were subjected to quantitative phytochemical analysis and different anti-oxidant analytical procedures like FRAP, DPPH etc to determine the radical scavenging capabilities. The results of phytochemical analysis estimated the quantities and revealed the presence of alkaloids, flavonoids, tannins, saponins and terpenoids which varied in both extracts. The methanol and aqueous extracts exhibited antioxidant activities with relatively high IC50 (IC50 = 3.67 ìg/ml and 4.42ìg/ml respectively) which accounted for a low free radical-scavenging activity when compared with the reference antioxidant, vitamin C (IC50 = 1.51ìg/ml). The results of the study indicates that A. godseffiana leaf extracts contain secondary metabolites and possesses antioxidant properties.

scholarly journals Assessment of in vitro Antioxidant and Antidiabetic Capacities of Medlar (Mespilus germanica)

2018 ◽  
Vol 47 (2) ◽  
pp. 384-389
Author(s):  
Sebnem Selen ISBILIR ◽  
Sevilay Inal KABALA ◽  
Hulya YAGAR
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Inhibitory Effect ◽  
Total Phenolic ◽  
Flower Bud ◽  
Ethanol Extracts ◽  
Dpph Scavenging ◽  
Β Carotene

The objective of the current study was to evaluate the antioxidant activity and enzyme inhibitory effect of different parts of medlar including fruit, leaf and flower bud by using various in vitro methods, and also determination of total phenolic and flavonoid content in the samples. Ethanol extracts of medlar parts were prepared and their antioxidant activities were determined using 1,1-diphenyl-2-picryl-hydrazil (DPPH•) scavenging and β-carotene bleaching methods. The leaf extract showed the strongest antioxidant activity. DPPHradical scavenging activity was in the order of BHA > leaf > bud > fruit. This ordering was the same for β-carotene bleaching activity, tocopherol > leaf > bud > fruit. The highest total phenolic (60.3 ± 1.69 mg GAE g-1 extract) and flavonoid (14.77 ± 1.15 mg QE g-1 extract) content were determined in leaf extract. For possible antidiabetic effects of extracts, α-amylase and α-glucosidase inhibitory activities were investigated, the bud extract showed the highest inhibition activities among the all extracts.

scholarly journals Production of eugenol from fungal endophytesNeopestalotiopsissp. andDiaporthesp. isolated fromCinnamomum loureiroileaves

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6427 ◽  
Author(s):  
Chutima Tanapichatsakul ◽  
Sarunpron Khruengsai ◽  
Sakon Monggoot ◽  
Patcharee Pripdeevech
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Endophytic Fungi ◽  
Antioxidant Activities ◽  
Chemical Constituents ◽  
Fungal Endophytes ◽  
Biologically Active ◽  
Crude Extracts ◽  
Antioxidant Agents ◽  
Fungal Extracts

Endophytic fungi, which colonize within a host plant without causing any apparent diseases, have been considered as an important source of bioactive secondary metabolites containing antimicrobial and antioxidant activities. The aim of this research was to isolate the endophytic fungi ofCinnamomum loureiroiand then to screen their antimicrobial and antioxidant activities. A total of 11 fungal endophytes were isolated from healthy leaves ofCinnamomum loureiroibelonging to six genera:Botryosphaeria,Colletotrichum,Diaporthe,Fusarium,Neopestalotiopsis, andPestalotiopsis. All isolated strains were cultured and further extracted with ethyl acetate solvent. Antimicrobial activity of all crude endophytic fungal extracts was analyzed using disc diffusion assay against six bacterial and two fungal pathogens. Crude extracts of strains MFLUCC15-1130 and MFLUCC15-1131 showed broad-spectrum antimicrobial activity against all tested pathogens. Activity againstBacillus cereusandStaphylococcus epidermidiswas notable, showing the lowest minimum inhibitory concentration at 3.91 μg/mL. Antioxidant activity of all crude endophytic fungal extracts was also evaluated based on 2,2-diphenyl-1-picrylhydrazyl assay. Significant antioxidant activity was detected in the crude extracts of fungus MFLUCC15-1130 and MFLUCC15-1131 with IC50of 22.92 ± 0.67 and 37.61 ± 0.49 μg/mL, respectively. Using molecular identification, MFLUCC15-1130 and MFLUCC15-1131 were identified asNeopestalotiopsissp. andDiaporthesp., respectively. The major chemical constituents produced by both crude extracts were identified by gas chromatography-mass spectrometry. Eugenol, myristaldehyde, lauric acid, and caprylic acid were the primary antimicrobial and antioxidant compounds in both crude extracts. This is the first report of eugenol being a biologically active compound ofNeopestalotiopsissp. andDiaporthesp. fungal endophytes. Eugenol has been reported as antimicrobial and antioxidant agents with agronomic applications. Thus the two newly-isolated endophytes may be used for eugenol production, which in turn can be used in a variety of applications.

scholarly journals Chemical profiling and antioxidant activities of essential oil from rhizomes of Acorus calamus L.

BIBECHANA ◽  
2020 ◽  
Vol 17 ◽  
pp. 89-95
Author(s):  
Sumnath Khanal ◽  
Devi Prasad Bhandari ◽  
Laxman Bhandari ◽  
Achyut Adhikari
Keyword(s):  
Essential Oil ◽  
Antioxidant Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Acorus Calamus ◽  
Chemical Compositions ◽  
Chemical Profiling ◽  
Ic50 Value ◽  
Sweet Flag ◽  
Dpph Scavenging ◽  
Dpph Scavenging Assay

Background and Aims: Acorus calamus L. is an indigenous herb in Nepal. It belongs to family Acoraceae and grows in wet land with scented rhizomes. It is also known as Sweet flag in English and commonly as Bojho in Nepal. The present investigation reveals the chemical compositions and antioxidant activity of rhizome essential oil of A. calamus. Methods: Essential oil of rhizomes of Acorus calamus L. from Kaski district, Nepal was extracted by hydrodistillation method and volatile constituents were analyzed Gas chromatography-Mass spectrometry. The antioxidant potential of essential oil was analyzed by 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) scavenging assay. Results: A GC-MS analysis revealed the presence of β-asarone (22.38%), α-asarone (14.97%), 1-(4,6-dimethoxy-2,3-dimethylphenyl ethanone (14.24%), Isoelemicin (5.68%), cis-Methylisoeugenol (4.26%), α-calacorene (4.16%), and other 20 minor components. From DPPH assay, half maximal inhibitory concentration (IC50) value of essential oil was found to be 108.71 µg/mL. Conclusions: These findings have strengthened the A. calamus L. is good source of compounds like β-asarone, α-asarone and can be used as potential antioxidants. BIBECHANA 17 (2020) 89-95

scholarly journals Evaluation of the Antioxidant and Antiradical Properties of Some Phyto and Mammalian Lignans

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7099
Author(s):  
Leyla Polat Kose ◽  
İlhami Gulcin
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Nordihydroguaiaretic Acid ◽  
Butylated Hydroxytoluene ◽  
Frap Assay ◽  
Antiradical Properties ◽  
Reducing Ability ◽  
Ic50 Values ◽  
Dpph Scavenging ◽  
Dpph Scavenging Assay

In this study, the antioxidant and antiradical properties of some phyto lignans (nordihydroguaiaretic acid, secoisolariciresinol, secoisolariciresinol diglycoside, and α-(-)-conidendrin) and mammalian lignans (enterodiol and enterolactone) were examined by different antioxidant assays. For this purpose, radical scavenging activities of phyto and mammalian lignans were realized by 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical (ABTS•+) scavenging assay and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging assay. Additionally, the reducing ability of phyto and mammalian lignans were evaluated by cupric ions (Cu2+) reducing (CUPRAC) ability, and ferric ions (Fe3+) and [Fe3+-(TPTZ)2]3+ complex reducing (FRAP) abilities. Also, half maximal inhibitory concentration (IC50) values were determined and reported for DPPH• and ABTS•+ scavenging influences of all of the lignan molecules. The absorbances of the lignans were found in the range of 0.150–2.320 for Fe3+ reducing, in the range of 0.040–2.090 for Cu2+ reducing, and in the range of 0.360–1.810 for the FRAP assay. On the other hand, the IC50 values of phyto and mammalian lignans were determined in the ranges of 6.601–932.167 µg/mL for DPPH• scavenging and 13.007–27.829 µg/mL for ABTS•+ scavenging. In all of the used bioanalytical methods, phyto lignans, as secondary metabolites in plants, demonstrated considerably higher antioxidant activity compared to that of mammalian lignans. In addition, it was observed that enterodiol and enterolactone exhibited relatively weaker antioxidant activities when compared to phyto lignans or standard antioxidants, including butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), Trolox, and α-tocopherol.

scholarly journals Study of Some Functional Properties and Antioxidant Activity of Two Types of Cherry Trees (Prunus avium) Gum Exudates Grown in Syria

2020 ◽  
pp. 13-22
Author(s):  
Tahani Al-idee ◽  
Hoda Habbal ◽  
Francois Karabt ◽  
Hussien Alzubi
Keyword(s):  
Antioxidant Activity ◽  
Functional Properties ◽  
Prunus Avium ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Hydroxyl Groups ◽  
Power Method ◽  
Arabic Gum ◽  
Dpph Scavenging ◽  
Emulsifying Capacity

The aim of this research is to study some functional properties and the antioxidant activity of cherry gum, collected from Serghaya and Suwayda in Syria, and to compare these features with those of Arabic gum. The values of the hydroxyl groups for the Arabic gum, Serghaya and Suwayda cherry gums were 757.1, 655.1 and 564.3 mg KOH/gm, respectively. The solubility of exudate gums ranged from 53.53 to 86.53% and was arranged as follows: Arabic gum>Serghaya cherry gum >Suwayda cherry gum. Gum solubility increased with rising the temperature. Water and oil holding capacities of cherry gums were significantly higher (p<0.05) than those of Arabic gum, while their emulsifying capacity was significantly lower than that of Arabic gum. The antioxidant activity of the gum was evaluated using the DPPH scavenging activity method and reducing power method. The inhibition percentages of DPPH were 51.52%, 23.23% and 21.19% for Serghaya, Suwayda and Arabic gums, respectively. The antioxidant activities of Serghaya and Suwayda gums using the reducing power method were 753.12 and 312.93 meq ascorbic acid/100gm, whereas Arabic gum didnot exhibit any Fe+3 reducing activity. All of gum types showed similar FT-IR spectra.

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