Anticancer activity of leaves extracts of Convolvulus pluricaulis and Mimosa rubicaulis (Lam.) by Brine Shrimp Lethality Bioassay

2021 ◽  
pp. 5427-5432
Author(s):  
Ashpak. M. Tamboli ◽  
Kiran. A. Wadkar
Keyword(s):  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Biologically Active ◽  
Cytotoxic Activities ◽  
Significant Activity ◽  
Active Components ◽  
Brine Shrimp Lethality ◽  
Ethanolic Extracts ◽  
Convolvulus Pluricaulis

This study was subjected to investigate brine shrimp lethality bioassay of Convolvulus pluricaulis and Mimosa rubicaulis (Lam.) in different polarities of leaves extracts. Brine shrimp organisms were used to assess the cytotoxic activities of various polarities of leaves extracts while using brine shrimp lethality bioassay the cytotoxicity had been assessed and correlated with standard. Five concentrations (20, 50,100,200,500µg/ml) of each extract were used to find out the cytotoxicity. The Artemia salina lethality bioassay result was evaluated in terms of LC50 (lethality concentration).In each plant extracts into three replicates concentration naupli were added. After 24 h the surviving Artemia salina larvae were counted and LC50 was assessed. The cytotoxic activity of all polarities of extracts displayed activity within the value of LC50 (132.10–266.76 µg/ml) for Convolvulus pluricaulis and (213.86–279.47 µg/ml) for Mimosa rubicaulis (Lam.) Results revealed that the ethyl acetate extracts of Convolvulus pluricaulis and ethanolic extracts of Mimosa rubicaulis (Lam.) elicit significant activity compared to other extracts it indicated biologically active components are present in ethyl acetate and ethanolic extracts that could be accounted for its pharmacological effects.

scholarly journals Uji Toksisitas Akut Ekstrak dan Fraksi Kulit Batang Ketapang Laut (Terminalia Catappa L.) Menggunakan Metode BSLT

2020 ◽  
Vol 6 (1) ◽  
pp. 42
Author(s):  
Ari Sartinah ◽  
Yamin Yamin ◽  
Muhammad Arba ◽  
Nur Illiyyin Akib ◽  
Andi Nafisah Tendri Adjeng ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Ethyl Acetate ◽  
Program Analysis ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Test Method ◽  
Brine Shrimp Lethality ◽  
Terminalia Catappa ◽  
Water Fractions

AbstrakToksisitas akut merupakan kemampuan suatu bahan kimia (obat/bahan obat) dalam menimbulkan kerusakan  pada suatu organisme dalam waktu yang relatif singkat. Tujuan penelitian ini adalah untuk mengetahui potensi ketoksikan akut dari ekstrak metanol, fraksi etil asetat dan fraksi air kulit batang ketapang laut (Terminalia catappa L.). Pengujian toksisitas akut dilakukan dengan menggunakan metode BSLT (Brine Shrimp Lethality Test) dan menggunakan analisis program untuk mengetahui nilai LC50 dari ekstrak dan fraksi. Hasil uji toksisitas akut menunjukan nilai LC50  dari ekstrak metanol, fraksi etil asetat dan fraksi air kulit batang ketapang laut (Terminalia catappa L. )  berturut-turut 247,997 ppm; 400,666 ppm dan 618,046 ppm. Hal ini menunjukan bahwa ekstrak metanol batang ketapang laut (Terminalia catappa L.) bersifat paling toksik dibandingkan fraksi etil asetat dan fraksi air.Kata kunci: Ekstrak, Fraksi, Terminalia catappa L., BSLT, Artemia salina LeachAbstractAcute toxicity is the ability of chemical (drug/drug ingredient) to cause damage to organisms in a relatively short time. The purpose of this study was to determine the potential for acute toxicity of methanol extract, ethyl acetate and water fractions of sea ketapang stem skin (Terminalia catappa L.). Acute toxicity testing was performed using the BSLT (Brine Shrimp Lethality Test) method and program analysis to determine the LC50 values of extracts and fractions. The results of the acute toxicity test showed the LC50 values of methanol extract, ethyl acetate and water fractions of the plant stem skin consecutively were 247,997 ppm; 400,666 ppm and 618,046 ppm. This shows that the methanol extract of sea ketapang stems (Terminalia catappa L.) is the most toxic compared to the ethyl acetate and the water fraction.Keywords: Extract, Fraction, Terminalia catappa L., BSLT, Artemia salina Leach

scholarly journals A Cytotoxic Activity of Tahongai (Kleinhovia hospita Linn.) Leaves Extracts Using Brine Shrimp Lethality Test

2019 ◽  
Vol 4 (3) ◽  
pp. 60
Author(s):  
Indah Solihah ◽  
Herlina Herlina ◽  
Riana Sari Puspita Rasyid ◽  
Tri Suciati ◽  
Khairunnisa Khairunnisa
Keyword(s):  
Statistical Analysis ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Brine Shrimp Lethality ◽  
Ethanol Extracts

Tahongai (Kleinhovia hospita Linn.) leaves had been known contain alkaloids, flavonoids, saponins, steroids, and tannins. At Komering, South Sumatera tribe, tahongai leaves had been known to treat tumor, cancer, polyps, acne, and dysmenorrhea. The study of cytotoxic activity of tahongai bark and stem were done. This study aims to determine the cytotoxic activity of tahongai leaves extracts using BSLT method. Tahongai leaves were extracted using gradual maceration with n-hexane, ethyl acetate, and ethanol 96%. Each extract was tested cytotoxic activity towards Artemia salina L. larvae. The yield of n-hexane, ethyl acetate, and ethanol extracts are 2,686%, 7,033%, and 7,933% respectively. Ethanol extract of tahongai leaves had the best cytotoxic activity with lethality value 76,667% at 500ppm. Statistical analysis with two way ANOVA showed extract and concentration had a significant (p<0,05) effect on larvae lethality percentage.

scholarly journals Analisis Fitokimia Dan Toksisitas Dari Ekstrak Daun Tumbuhan Mempening (Lithocarpus bancanus (Scheff.) Rehd) Menggunakan Metode BSLT (Brine Shrimp Lethality Test)

2019 ◽  
Vol 2 (2) ◽  
pp. 29-33
Author(s):  
Retno Puji Lestari ◽  
Rudi Hendra Sy ◽  
Hilwan Yuda Teruna
Keyword(s):  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Analysis ◽  
Brine Shrimp Lethality ◽  
The Family ◽  
Tribal Region

Mempening (Lithocarpus bancanus (Scheff.) Rehd) is a species belongs to the family Fagaceae which is found growing around the Talang Mamak tribal region in Kelayang District, Indragiri Hulu Regency, Riau Province. The purpose of this study was to determine the toxicity of leaf extract of L. bancanus (Scheff.) Rehd against Artemia salina Leach larvae using the Brine Shrimp Lethality Test (BSLT) method and its phytochemical analysis. The results of the toxicity was determined against n-hexane, dichloromethane, ethyl acetate, methanol, and ethanol extract and they showed LC50 values ​​of 3.15 ppm; 41163.26 ppm; 5817.09 ppm; 817, 27 ppm; 9019.70 ppm. Phytochemical analysis of the leaves of L. bancanus (Scheff.) Rehd plant showed a class of flavonoids, terpenoids, phenolics and saponins.

scholarly journals Evaluation of antioxidant activity and cytotoxic potential of Cryp-tocoryne ciliata

2013 ◽  
Vol 2 (2) ◽  
pp. 38-41
Author(s):  
Ummey Jannatun Nahar ◽  
Shah Marzia Mahjabin Lina
Keyword(s):  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Methanolic Extract ◽  
Soluble Fraction ◽  
Cytotoxic Activities ◽  
Brine Shrimp Lethality ◽  
Whole Plant ◽  
Vincristine Sulphate

Cryptocoryne ciliata belonging to the Araceae family has been investigated for isolation of its secondary metabolites and evaluation of biological activities of the crude extractives with special emphasis to the antioxidant activity and brine shrimp lethality bioassay. The whole plant was extracted with methanol and concentrated extract was partitioned using petroleum ether, carbon tetrachloride and ethyl acetate. Aqueous soluble fraction of the methanolic extract showed the highest antioxidant activity. The carbon tetrachloride soluble fraction of the methanolic extract and the ethyl acetate soluble fraction of the methanolic extract showed moderate antioxidant activity as compared to free antioxidant activity of tert-butyl-1-hydroxytolunene. In the brine shrimp lethality bioassay, among all extracts of whole plant of C. ciliata, the carbon tetrachloride soluble fraction of the methanolic extract showed strong cytotoxic activity. Aqueous soluble fraction of the methanolic extract, methanolic crude and ethyl acetate soluble fraction of the methanolic extract showed mild cytotoxity as compared to that of vincristine sulphate. The study confirms the mild to moderate antioxidant and moderate potent cytotoxic activities of C. ciliata plant extract as compared to reference standards and therefore demands the isolation of active principles and thorough bioassay.DOI: http://dx.doi.org/10.3329/icpj.v2i2.13196 International Current Pharmaceutical Journal 2013, 2(2): 38-41

scholarly journals Cytotoxic Activity of Parmelia perlata Extracts against Artemia Salina

2016 ◽  
pp. 36-39
Author(s):  
M. Mayilsamy ◽  
K. Geetharamanan
Keyword(s):  
Ethyl Acetate ◽  
Plant Extracts ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Cytotoxic Activities ◽  
Preliminary Assessment ◽  
Cytotoxic Potential ◽  
Antitumor Properties ◽  
Bio Assay

The brine shrimp lethality bio assay represents a rapid, inexpensive and simple bioassay for testing plant extracts bioactivity which in most cases correlates reasonably well with cytotoxic and antitumor properties. The assay is considered to be a very useful tool for preliminary assessment of toxicity. In the present study the lichen Parmelia perlata was extracted with solvents of varying polarity such as hexane, ethyl acetate, acetone and methanol and tested for hatch inhibition of cysts of Artemia salina and cytotoxic activities against Artemia salina naupilii. The highest cytotoxic potential among all the plant extracts tested was explored from the hexane extract of P. perlata which showed 100% brine shrimp mortality at 100ppm. The least activity among all the plant extracts was found in the methanol extract of  P. perlata.

scholarly journals Chemical and Biological Studies of Leaf Extract of Dendrophthoe falcata Linn.

2018 ◽  
Vol 16 (2) ◽  
pp. 215-219 ◽  
Author(s):  
Md Rajdoula Rafe ◽  
Monira Ahsan ◽  
Choudhury Mahmood Hasan ◽  
Mohammad Mehedi Masud
Keyword(s):  
Brine Shrimp ◽  
Methanol Extract ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Cytotoxic Activities ◽  
Significant Activity ◽  
Brine Shrimp Lethality ◽  
Biological Studies ◽  
Dendrophthoe Falcata

Dendrophthoe falcata (Family Loranthaceae) is used extensively in rural area as a component of ethno-medicine for the treatment of various diseases. In this study, the crude extracts and the fractions obtained from D. falcata were investigated for potential chemical constituents and some biological activities. For medicinal properties the antioxidant, brine shrimp lethality and thrombolytic activities have been investigated. The structures of the isolated three compounds were solved by extensive analyses of their high resolution 1HNMR spectroscopic data. They were identified as Lupeol, 3-β-acetoxy-12-ene-11-one and β-sitosterol. For bioactivities, the petroleum ether, dichloromethane, chloroform and aqueous soluble fractions abbreviated as PESF, DCMSF, CSF and AQSF respectively. Brine shrimp lethality bioassay was used to evaluate potential cytotoxic activities, where all fractions showed significant activity with lower LC50. Most significant activity has been observed for methanol extract (LC50= 4.477 µg/ml). AQSF revealed maximum activity in DPPH free radical scavenging assay (IC50 = of 43.49 μg/ml). In assay for thrombolytic activity, the methanol extract and its chloroform soluble fraction demonstrated significant efficacy with 32.65% and 32.36% clot lysis, respectively.Dhaka Univ. J. Pharm. Sci. 16(2): 215-219, 2017 (December)

scholarly journals KAJIAN TOKSISITAS DARI TUBUH BUAH Ganoderma lucidum DENGAN METODE BRINE SHRIMP LETHALITY TEST (BST)

2014 ◽  
Vol 1 (1) ◽  
pp. 32
Author(s):  
Ross Nurul Rohmah ◽  
Nuniek Ina Ratnaningtyas ◽  
Ari Asnani
Keyword(s):  
Toxic Effect ◽  
Fruiting Body ◽  
Brine Shrimp ◽  
Ganoderma Lucidum ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Analysis ◽  
Brine Shrimp Lethality ◽  
One Stage ◽  
Body Extract

Ganoderma lucidum is polyporus fungi from Basidiomycetes which can be used as traditional medicines. Toxicity test with Brine Shrimp Lethality Test (BST) method using Artemia salina was conducted to find out toxic effect of G.lucidum. An extract would have toxic effect if the LC50 < 1000 µg/ml. The aims of this experiment were to know about the toxicity level from G.lucidum with Brine Shrimp Lethality Test (BST) method and determined the concentration of fruiting body extract of G.lucidum which had the best toxicity effect in LC50. There were two extraction methods used in this experiment, first one stage extraction and then multilevel extraction with hexane, ethyl acetate, and ethanol. Each solvent was made in 1000 ppm, 500 ppm, 250 ppm, and 125 ppm. This process was repeated for three times. This experiment used G.lucidum extract which was tested to A.salina and secunder compound metabolit test from the most toxic G. lucidum was done with phytochemical analysis. The result indicated that fruiting body extract from G.lucidum could kill A.salina because all extract was positively contained alkaloid and terpenoid but negatively contained flavonoid. Fruiting body extract from G.lucidum which had lowest LC50 wass ethyl asetat extract with one stage extraction in concentration 53,70 ppm and highest LC50 was ethanol extract with multilevel extraction in concentration 501,18 ppm.

scholarly journals Skrining antikanker menggunakan metode BST (Brine Shrimp Lethality Test) pada ekstrak metanol daun saga (Abrus precatorius L.) dengan partisi etanol

2018 ◽  
Vol 7 (1) ◽  
pp. 61
Author(s):  
Kurnia Ritma Dhanti
Keyword(s):  
Brine Shrimp ◽  
Artemia Salina ◽  
Brine Shrimp Lethality ◽  
Abrus Precatorius

Latar belakang : Pengembangan senyawa antikanker dari bahan alami perlu dilakukan untuk meminimalisir efek samping dari penanganan penyakit kanker yang saat ini banyak dilakukan. Suatu senyawa dapat diketahui potensi antikankernya dengan pendekatan menggunakan metode BST (Brine Shrimp Lethality Test). Ekstrak metanol daun saga (Abrus precatorius L.) bersifat toksik terhadap larva A. salina Leach.   Tujuan : Mengetahui bagian teraktif dari ekstrak metanol daun saga yang dipartisi (dipisahkan) menggunakan pelarut etanol.   Metode : Ekstrak metanol daun saga dipartisi dengan pelarut etanol hingga terbentuk bagian larut dan tidak larut. Kedua bagian tersebut diuji menggunakan metode BST dengan 5 kali ulangan dan 3 replikasi yang masing-masing menggunakan 10  ekor larva A.salina.   Hasil : Dari perhitungan didapatkan nilai LC50 bagian larut etanol sebesar 144,544 ppm sedangkan nilai LC50 bagian tidak larut etanol sebesar 151,356 ppm.   Kesimpulan : Bagian larut etanol menyebabkan persentase kematian yang lebih tinggi daripada bagian tidak larut. Nilai LC50 bagian larut etanol lebih rendah dibanding bagian tidak larutnya. Semakin rendah nilai LC50 senyawa, maka semakin berpotensi pula untuk dikembangkan sebagai agen antikanker.   Kata kunci : toksisitas, Abrus precatorius L., Artemia salina Leach., antikanker, partisi. 

scholarly journals UJI TOKSISITAS DARI EKSTRAK LAMUN JENIS Thalassia hemprichii DARI PERAIRAN KALASEY DENGAN MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST

2019 ◽  
Vol 7 (3) ◽  
pp. 265
Author(s):  
Faradila Y. Karim ◽  
Nickson J Kawung ◽  
Billy Th. Wagey
Keyword(s):  
Cytotoxic Activity ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Bioactive Compound ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Thalassia Hemprichii ◽  
Brine Shrimp Lethality ◽  
A Value ◽  
Small Doses

ABSTRACTBioactive compounds that are suspected of having anticancer activity are first tested for activity by means of a toxicity test. The purpose of this test is to obtain data on the ability of the activity of a bioactive compound to kill cells at small doses so as to obtain a lethal concentration or lethal data. These two measurements are often called LC50 or LD50, concentrations that can kill 50% of test animals. This study aims to test the cytotoxic activity of Thalassia hemprichii seagrass extract using the Brine Shrimp Lethality Test (BSLT) method.The results of the study showed that the mortality of Artemia salina larvae was influenced by the concentration of the test, meaning that the higher the concentration the more test animals died. Furthermore, the results of the probit analysis contained the toxicity activity of bio active compounds from seagrasses where the LC50 was 3.95 mg / l. A substance is declared to have the potential for cytotoxic activity if it has a value of LC50 at concentrations <1000 ppm for extracts and at concentrations <30 ppm for a compound. Keywords: Cytotoxic Activity, Thalassia hemprichii, Brine shrimp Lethality Test (BSLT)  

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