scholarly journals Evaluation of the Interaction Between Phytase and Xilanase on Metabolizability of Energy-Reduced Broiler Diets

2020 ◽  
Vol 8 (3) ◽  
pp. 515
Author(s):  
Natália Ramos Batista Chaves ◽  
Karina Marcia Ribeiro de Souza Nascimento ◽  
Charles Kiefer ◽  
Maurício Silva Rosa ◽  
Henrique Barbosa de Freitas ◽  
...  

To examine the association between phytase and xylanase in diets with nutritional adjustments on the metabolizability of feed by broiler diets, 250 chickens were used and distributed among five treatments and five replicates of 10 broilers each. The treatments were: positive control diet - without phytase or xylanase; negative control diet - with an energy reduction of 0.419 MJ/kg, without phytase or xylanase; and three diets containing xylanase and phytase and energy reductions of 0.209, 0.409 and 0.628 MJ/kg. For all energy-reduced diets, the nutritional matrix of phytase with phosphorus (0.15%), calcium (0.165%) and sodium (0.035%) was considered. The energy reduction of 0.628 MJ/kg with inclusion of the enzymes resulted in a higher metabolizability of the crude protein, also the apparent and apparent corrected metabolizable energy coefficients for nitrogen balance were higher in diets with a reduction energy of 0.209 MJ/kg, however had values similar to those obtained in the positive control diets and diets with energy reduction of 0.628 MJ/kg containing enzymes. The inclusion of phytase (500 FTU/kg) and xylanase (16,000 BXU/kg) in broiler diets, makes it possible to reduce metabolizable energy by up to 0.628 MJ/kg, available phosphorus, calcium, and sodium, and improve the metabolizability of the nutrients.

2018 ◽  
Vol 31 (3) ◽  
pp. 748-758 ◽  
Author(s):  
NATÁLIA YOKO SITANAKA ◽  
FÁBIO ENRIQUE LEMOS BUDIÑO ◽  
SIMONE RAYMUNDO DE OLIVEIRA ◽  
ANDREIA DONIZETE CHAGAS VILAS BOAS ◽  
JOSÉ EVANDRO DE MORAES

ABSTRACT The objective of this study was to evaluate the use of an enzyme complex-α-amylase, ß-glucanase, phytase, cellulase, xylanase and protease-in the feed of swine in growing and finishing phases, by assessing their performance (daily weight gain, daily feed intake, and feed conversion), dry matter, crude protein, and crude energy apparent digestibility, and the feed costs. Eighty pigs-castrated males, and females-of approximately 63 days of age and initial weight of 20.18±1.98 kg were distributed in a 5×2 factorial arrangement (diet × sex), in a randomized block design, with eight replications. The treatments used were: positive control diet consisted of 3,300 kcal/kg in the growth-I phase, and 3,250 kcal/kg in the growth-II and finishing phases; negative control diet with energy reduction of 85 kcal/kg of metabolizable energy (ME) (NC85); NC85 diet with addition of enzyme complex (EC85); negative control diet with energy reduction of 100 kcal/kg ME (NC100); NC100 diet with addition of enzyme complex (EC100). The addition of the enzyme complex to the diet with energy reduction of 100 kcal/kg ME increased the digestible protein content of the diet for the swine in the growth-II phase. The addition of the enzyme complex to the diet with energy reduction of 85 and 100 kcal/kg ME increased the digestible energy content of the diets for the swine in the finishing phase. The use of enzyme complex in diets with reduction of 85 kcal/kg ME for male swine in growing and finishing phases is recommended for improving feed conversion and economic efficiency during the growth-I phase. The use of enzyme complex is not justified for female swine.


2019 ◽  
Vol 40 (2) ◽  
pp. 767
Author(s):  
Gabriel Villela Dessimoni ◽  
Nilva Kazue Sakomura ◽  
Daniella Carolina Zanardo Donato ◽  
Fábio Goldflus ◽  
Nayara Tavares Ferreira ◽  
...  

Although phytase has been researched, new enzymes have been produced, leading to different animal responses. In this scenario, the present study proposes to evaluate the inclusion of a bacterial phytase produced by Escherichia coli in broiler diets based on corn and soybean meal, with or without nutrient reductions, on the performance, nutrient digestibility, phosphorus bioavailability, and bone minerals of those animals and on the economic viability of this practice. A total of 896 male broiler chickens were distributed into 32 experimental units, each housing 28 broilers. The experiment was set up as a completely randomized design with four treatments (Positive Control (PC)- diet meeting the nutritional requirements of the broiler chickens; Negative Control (NC) with reductions of 100kcal/kg of ME, 0.14% avP and 0.11% tCa; NC + phytase (500 FTU/kg); PC + phytase (500FTU/kg)) and 8 replicates. Phytase increased (p < 0.05) feed intake and body weight gain and improved feed conversion ratio in starter (1 to 21 days) and total (1 to 42 days) phases, respectively, compared with Negative Control diet without supplementation. The Negative Control + phytase diet also led to a feed intake similar to Positive Control in the starter and total phases. The inclusion of phytase without nutrient reductions improved (p < 0.05) feed conversion ratio in the starter phase, compared to Positive Control diet. There was an increase (p < 0.05) in the digestibility of dry matter, crude protein and phosphorus and in apparent digestible energy in the Negative Control + phytase diet compared to the Negative Control diet. Phytase supplementation increased (p < 0.05) the digestibility of crude protein, calcium and phosphorus, and apparent digestible energy compared to Positive Control diet. Ash, phosphorus, and calcium contents were higher in the Negative Control + phytase diet compared with those observed in the Negative Control diet without enzyme (p < 0.05). The Positive Control + phytase diet provided higher ash contents (p < 0.05), but calcium and phosphorus deposition was similar to those obtained with Positive Control diet. Phytase inclusion allows for a reduction in the diet cost per ton of produced feed. In conclusion, dietary supplementation with bacterial phytase produced from Escherichia coli for broiler chickens is recommended, as it provided increases in production performance, nutrient digestibility, and energy metabolizability and a reduction in the diet cost.


2010 ◽  
Vol 39 (6) ◽  
pp. 1237-1245 ◽  
Author(s):  
Adimar Cardoso Júnior ◽  
Paulo Borges Rodrigues ◽  
Antônio Gilberto Bertechini ◽  
Rilke Tadeu Fonseca de Freitas ◽  
Renato Ribeiro de Lima ◽  
...  

Two experiments using Cobb broiler chicks from 8 to 35 days of age were carried out to evaluate the effect of reducing levels of calcium and available phosphorus in diets supplemented with 500 ftu phytase/kg on the performance of the birds, the content of ashes in the tibia, the metabolizable energy of the diet, and the metabolizability of the dry matter and crude protein. It was used 1,404 broiler chicks in the experiment 1 to evaluate feed intake, weight gain, food conversion and percentage of the ashes in the tibia. In the experiment 2, simultaneously carried out with experiment 1, a total of 390 birds were transferred to a metabolism room to determine the metabolizable energy and metabolizability of the dry matter and crude protein. It was used in the two experiments, a complete random design in a 3 × 4 + 1 factorial scheme with three levels of available phosphorus (0.375; 0.325; and 0.275%) and four levels of calcium (0.85; 0.75; 0.65 and 0.55%) in the diets, supplemented with phytase. It was also used a control diet without phytase, based on corn and soybean bran, formulated with 0.425% of available phosphorus and 0.85% of calcium. In the experiment 1, the reduction of levels of calcium and phosphorus did not cause a significant difference on the performance and percentage of bone ashes. The levels of calcium and available phosphorus used in the experiment 2 can be reduced down to 0.65 and 0.325%, respectively, because the effects are similar to those obtained with the currently suggested levels.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 204-205
Author(s):  
Kelsey L Batson ◽  
Hilda I Calderon Cartagena ◽  
Mike D Tokach ◽  
Jason C Woodworth ◽  
Robert D Goodband ◽  
...  

Abstract A total of 355 pigs (DNA 200x400; initially 5.6 kg) were used to evaluate the effects of coarse wheat bran and crude protein (CP) level in diets without pharmacological levels of zinc oxide on nursery pig performance and fecal dry matter (DM%). Pigs were allotted to 1 of 6 treatments in a completely randomized design with 5 pigs per pen and 12 pens per treatment. Treatment diets were fed in two phases (d 0 to 7 and 7 to 21) followed by a common diet fed d 21 to 45. Treatments included a positive control diet with ZnO, negative control without ZnO, and negative control with 4% wheat bran and formulated to contain 21, 19.5, 18, or 16.5% CP. All diets contained 1.35% SID lysine except the 18 and 16.5% (1.25 and 1.20% lysine) CP diets. Data were analyzed using the lmer function from the lme4 package in R. During the experimental period, decreasing CP decreased (linear, P&lt; 0.05) ADG, G:F, and d 21 BW, but increased (linear, P=0.005) fecal DM%. Pigs fed the diet containing ZnO had increased (P&lt; 0.050) ADG, ADFI, and G:F compared to the negative control and diet with 21% CP and wheat bran. From d 21 to 45, pigs previously fed the 21% CP diet with wheat bran had increased (P&lt; 0.05) ADG compared to the positive control. Fecal DM on d 45 and G:F increased (P&lt; 0.05) for pigs fed the 21% CP, wheat bran diet and negative control compared to those fed the positive control. Overall, pigs fed low CP diets had decreased (P&lt; 0.05) ADG while pigs fed the positive control had greater (P&lt; 0.05) ADG compared to the negative control. Additional research is needed to determine if modifying low CP, wheat bran diets will elicit benefits of increased fecal DM% while maintaining growth performance.


2019 ◽  
Vol 19 (1) ◽  
pp. 159-172 ◽  
Author(s):  
Tércia Cesária Reis de Souza ◽  
Araceli Aguilera Barreyro ◽  
Sara Rubio Rubio ◽  
Yanier Machado González ◽  
Konisgmar Escobar García ◽  
...  

AbstractTwo experiments were conducted to test if dehydrated porcine plasma (DPP) and potato protein concentrate (PPC) could be used as an alternative to antibiotics in starter diets for piglets. Experiment one was conducted to test if DPP and PPC in an antibiotic-free diet affected pig performance, and faecal consistency. Eighty-four piglets weaned at 22 days and weighing 6.9 kg were used. Piglets were fed for two weeks with one of four diets: a positive control diet with antibiotics (C+); and three other diets without antibiotics added with DPP, PPC, or DPP and PPC (DPP+PPC) to measure the average daily feed intake (ADFI), average daily gain (ADG), feed efficiency (FE), and incidence and severity of diarrhoea (ID and ISD respectively). In experiment two, twenty-four piglets weaned at 17 days and weighing 5.7 kg, were implanted at 21 days of age with a T-cannula at the terminal ileum to measure the apparent ileal digestibility (AID) and the apparent total tract digestibility (ATTD) of nutrients. Piglets were fed one of four diets: a positive control diet with antibiotics (C+); a negative control diet without antibiotics (C–), and two diets without antibiotics added with DPP, or PPC. The results of experiment one showed that the DPP diet was the most consumed diet during the first week, and the ADG and FE were similar among treatments. During the second week and the total experimental period the ADFI, ADG, and FE were similar among diets. The ID was lower in the C+ diet than other diets. The ISD was lower in the C+ diet than DPP and DPP+PPC diets; piglets fed PPC diet were similar to piglets fed C+ and DPP and DPP+PPC diets. The results of digestibility showed that crude protein AID was higher in piglets fed C+ and PPC diets than C– and DPP diets. Dry matter ATTD and energy ATTD were higher for piglets fed PPC than other diets. Further, crude protein ATTD of DPP and PPC diets tended to have a similar digestibility to that of C+ diet. The results suggest that PPC is a potential controller of post-weaning diarrhoea.


2021 ◽  
Vol 42 (5) ◽  
pp. 3029-3046
Author(s):  
Rodrigo de Freitas Jacob ◽  
◽  
Luiz Fernando Teixeira Albino ◽  
Arele Arlindo Calderano ◽  
Romário Duarte Bernardes ◽  
...  

Three experiments were carried out to verify the effects of the enzyme phytase, alone or combined with an enzyme complex, in diets deficient in available phosphorus (AP), calcium (Ca), and metabolizable energy (ME) on broiler performance, ME, and dietary amino acid digestibility. A total of 1,538 male Cobb 500 broilers were allocated to the three experiments, each of which consisted of five treatments: positive control (PC; basal ration); negative control 1 (NC1; PC minus 0.15% of AP, 0.16% of Ca, and 68 kcal kg-1 ME); negative control 2 (NC2; PC minus 0.15% of AP, 0.16% of Ca, and 101 kcal kg-1 of ME); NC1 plus phytase; and NC2 plus phytase plus enzymatic complex. Body weight gain (WG) and feed intake were measured from 1-21 days and from 1-42 days, and the corrected feed conversion rate (FCR) for mortality was calculated. In the second and third experiments, the apparent ME corrected for nitrogen balance (AMEn) and standardized digestibility of amino acids, respectively, were determined, for the diets supplemented with phytase and the enzymatic complex. In the first experiment, enzyme supplementation increased (p < 0.05) WG at 21 days and 42 days relative to the negative controls. Phytase inclusion improved (p < 0.05) FCR at the initial phase compared to the NC1 diet. In the second experiment, enzyme supplementation did not affect (p > 0.05) AMEn. In the third experiment, both enzyme treatments improved (p < 0.05) the digestibility of amino acids in the supplemented diets compared to the deficient diets. Supplementation with phytase and carbohydrases preserves the performance of broilers fed diets deficient in AP, Ca, and ME and improves amino acid digestibility.


2020 ◽  
Vol 48 (2) ◽  
pp. 323-328
Author(s):  
Denis Rogério Sanches Alves ◽  
Suzana Raquel De Oliveira ◽  
Bruno Dos Santos Sosa ◽  
Wilson Rogério Boscolo ◽  
Altevir Signor ◽  
...  

This study determined the compelling palatability of Atractus AQVA® flavoring for Nile tilapia juveniles (Oreochromis niloticus). Five isoproteic (40% crude protein) and isoenergetic (3,420 kcal kg-1) experimental diets were elaborated containing 0.25 (A25), 0.50 (A50) and 0.75% (A75) flavoring inclusion, a positive control diet with 10% fishmeal (FPE) and a negative control without fishmeal (SPE). Five juvenile individuals (2.58 ± 0.27 g) were distributed in five 10 L tanks and were fed four times a day with one of the diets, randomly raffled. The same number of pellets was offered, and the following behaviors were observed: time to capture the first pellet, number of pellet rejections, and number of approximations without capture and consumed pellets in each feeding event, using three-minute recordings with a digital camera. A significant effect (P < 0.05) was found regarding the number of approximations without capturing the pellet, as well as a higher palatability index for A75, followed by A50, A25, SPE and FPE. Therefore, it was concluded that A75 provided the highest compelling palatability of all diets by increasing the palatability index by 10.49% and displaying a 23.13% reduced rejection of pellets, besides presenting a 3.3 fold reduction in the number of approximations without capturing pellets with diet FPE.


1990 ◽  
Vol 38 (3B) ◽  
pp. 597-607
Author(s):  
J.B. Schutte ◽  
M.W. Bosch ◽  
N.P. Lenis ◽  
J. de Jong ◽  
J.T.M. van Diepen

In 6 trials with 460 pigs, initially about 19 kg the threonine requirement for growth between about 20 to 40 kg was investigated. To a negative control diet, containing crude protein 160 g and threonine 5.6 g/kg. L-threonine 0.0, 0.6, 1.2 and 1.8 g/kg, was added and compared with a positive control diet with crude protein 185 g/kg and threonine 6.8 g/kg. The negative control diet was supplemented with lysine, methionine, tryptophan, isoleucine, histidine and valine so that no other amino acids than threonine were limiting. The positive control diet was supplemented with lysine and methionine. The experimental diets were fed ad libitum as pellets. In 2 separate trials, the apparent faecal and ileal digestibility of the amino acids of the 2 control diets was estimated. The requirement for total threonine was 7.1 g/kg for maximum weight gain, and 7.4 g/kg for maximum efficiency of feed utilization in a diet containing net energy 2290 kcal/kg. These results correspond with 6.2 and 6.5 g/kg faecal digestible and 5.7 and 6.0 g/kg ileal digestible threonine, respectively. Pig performance on the negative control diet supplemented with the first limiting amino acids, was similar to that of the positive control diet. (Abstract retrieved from CAB Abstracts by CABI’s permission)


2004 ◽  
Vol 79 (1) ◽  
pp. 149-153 ◽  
Author(s):  
N. R. Augspurger ◽  
D. H. Baker

AbstractThree pig trials were done to estimate an objective requirement of young (10 to 20 kg) pigs for leucine (Leu). Within each trial, four or five replicates of five pigs were given ad libitum access to their experimental diet for a period of 21 (trial 1) or 19 days (trials 2 and 3). A Leu-deficient diet (177 g crude protein per kg) based on maize, peanut meal, lactose, sucrose, dried whey, maize starch, soya-bean meal (SBM), and gelatin was used in these trials. Trial 1 showed that the Leu-deficient diet supplemented with Leu produced responses in weight gain and gain/food ratio that were similar (P > 0·10) to that obtained from a maize-SBM-whey positive-control diet containing 213 g crude protein (CP) per kg. Trials 2 and 3 utilized graded levels of crystalline L-Leu to determine the minimal Leu requirement in young pigs. Both trials showed linear increases (P < 0·05) in weight gain and gain/food as a result of Leu supplementation. Closer examination of the data revealed no significant change in gain/food or PUN responses above 11·2 g/kg total Leu in trial 2, or in weight gain or PUN values above 11·5 g/kg total Leu in trial 3. These data indicate that the Leu requirement of young pigs is probably not greater than 11·5 g/kg (10·5 g/kg true digestible), which equates to approximately 0·72 g true digestible Leu per MJ metabolizable energy. The ideal ratio of Leu to Lys appears to be close to 1: 1 for pigs in the weight range of 10 to 20 kg.


Author(s):  
Caroline Bavaresco ◽  
Everton Luis Krabbe ◽  
Diego Surek ◽  
Edenilse Gopinger ◽  
Fernando Nicolas Martinez ◽  
...  

Abstract The objective of this work was to evaluate the effect of the use of hybrid phytase, alone or combined with carbohydrases, in poultry diets with nutritional reductions of calcium, available phosphorus, and metabolizable energy on the nutrient digestibility and bone quality of broiler chickens. A total of 1,875 broilers were distributed in five treatments in a completely randomized design, with 15 replicates of 25 chickens each. The treatments consisted of a positive control feed (T1) and of four negative controls (T2 to T5): T1, basal diet (BD) with corn and soybean; T2 and T3, BDs with reductions of 70 and 100 kcal kg-1 metabolizable energy, respectively, and both with reductions of 0.16% Ca and 0.15% available P; and T4 and T5, BDs with the same nutritional reductions, but supplemented with enzymes, i.e., T4 = T2 + 500 phytase units (FTU) per kilogram and T5 = T3 + 500 FTU kg-1 + 560 xylanase units (TXU) per kilogram + 250 glucanase units (TGU) per kilogram. The use of 500 FTU kg-1 hybrid phytase in pelleted corn-soybean meal diets allows a good digestive performance by broilers and replaces the nitrogen-corrected apparent metabolizable energy at 70 kcal kg-1, as well as 0.16% Ca and 0.15% available P.


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