Assessment of antibacterial potentials of violacein extract from Chromobacterium violaceum isolated from domestic and recreational water sources in Owerri, Nigeria

2021 ◽  
Vol 1 (2) ◽  
pp. 007-012
Author(s):  
Lovelyn Chinyeaka Ugenyi ◽  
Joy Nkeiruka Dike-Ndudim ◽  
Henry Chidozie Amah ◽  
Chizaram Winners Ndubueze
Keyword(s):  
Bacterial Infections ◽  
Bacterial Count ◽  
Water Sources ◽  
Culture Broth ◽  
Chromobacterium Violaceum ◽  
Swimming Pool ◽  
Nutrient Agar ◽  
Recreational Water ◽  
Domestic Water ◽  
Bacterial Counts

This study was carried out with the aim of assessing the antibacterial potentials of violacein extracted from Chromobacterium violaceum isolated from domestic and recreational water sources in Owerri, Nigeria. Water samples were collected from different locations of the domestic water sources, five different swimming pools, and three borehole stations using sterile amber bottles. The isolation of C. violaceum was done using pour plate method on nutrient agar. The violet colonies of C. violaceum were counted, characterized and identified using standard microbiological and biochemical techniques. The mean viable bacterial counts were high. Water sample from Otamiri station-1 have the highest bacterial count (200 × 101 CFU/ml and 19.50 × 101 CFU/ml) respectively. Swimming pool 1 and 3 bacterial counts were (4.50 × 101 CFU/ml, 11 × 101 CFU/ml and 11.50 × 101 CFU/ml) respectively. For borehole 1, 2 and 3, swimming pool 2, 4 and 5, counts were (0.00 × 101 CFU/ml). Ethanolic extraction of violacein from C. violaceum was performed from a 48-hour culture broth. The sensitivity of the bacteria isolates to violacein was assayed on nutrient agar and nutrient broth by agar diffusion and broth dilution methods respectively. All the bacterial isolates were susceptible to the violacein extract at various concentrations, except MRSA that showed resistance to the violacein at 2.19mg/ml for extract from recreational water isolate and at 17.5mg/ml to 2.19mg/ml for extract from domestic water isolates. Conclusively, violacein has the potential to be used as an antibacterial compound for treatment of multidrug resistant bacterial infections.

scholarly journals Assessment of antifungal potentials of violacein extract from Chromobacterium violaceum isolated from domestic and recreational water sources in Owerri, Imo State, Nigeria

2021 ◽  
Vol 10 (3) ◽  
pp. 168-172
Author(s):  
Joy Nkeiruka Dike-Ndudim ◽  
Lovelyn Chinyeaka Ugenyi ◽  
Chizaram Winners Ndubueze
Keyword(s):  
Candida Albicans ◽  
Aspergillus Niger ◽  
Fungal Infections ◽  
Water Sources ◽  
Chromobacterium Violaceum ◽  
Swimming Pool ◽  
Recreational Water ◽  
Swimming Pools ◽  
Inhibitory Effects ◽  
Imo State

Anti-Microbial Resistance (AMR) in superficial fungal infections are major worldwide public health problem that affects a large part of the human population globally. The antifungal potentials of violacein extracted from Chromobacterium violaceum isolated from domestic and recreational water sources in Owerri, Imo State, Nigeria, was assessed. Three water samples were collected from different locations of the Otamiri River, five from different swimming pools and three from different borehole locations in Owerri Metropolis. The samples were cultured on nutrient agar by pour plate method. The violet colonies of Chromobacterium violaceum were counted, characterized and identified. Water sample from Otamiri River station-1 had the highest bacteria count (20.00 × 101 CFU/Ml and 19.50 × 101 CFU/mL) respectively. Swimming pool 1 and 3 bacterial counts were (14.50 × 101 CFU/mL, 11.00 × 101 CFU/mL and 11.50 × 101 CFU/mL) respectively. For borehole 1, 2 and 3, swimming pool 2, 4 and 5 counts were (0.00 × 101 CFU/mL). The ethanolic extracts from the isolates (violacein) and the control drug (fluconazole 50µg/mL) both had inhibitory effects on the test organisms (Candida albicans and Aspergillus niger) at different concentrations. The MIC of Fluconazole on Candida albicans and A. niger were 25µg/ml and 50µg/Ml, respectively. Violacein from both swimming pools and Otamiri River isolates had MIC of 8.75mg/ml on A. niger and MIC of 4.375mg/ml on Candida albicans. Violacein which proved to have inhibitory effects on Candida albicans and Aspergillus niger can be harnessed for treatment of infections caused by these fungi.

scholarly journals A field study of some swimming-pool waters with regard to bacteria, available chlorine and redox potential

1974 ◽  
Vol 72 (1) ◽  
pp. 101-110 ◽  
Author(s):  
Katarina Victorin
Keyword(s):  
Redox Potential ◽  
Bacterial Count ◽  
Swimming Pool ◽  
Total Bacterial Count ◽  
Aluminium Sulphate ◽  
Sand Filters ◽  
Bacterial Counts ◽  
Chlorine Gas ◽  
Swimming Pool Water

SUMMARYThe waters of one indoor bath and three outdoor baths were examined once an hour during 3 days (bath 1) or 6 days, for available chlorine, redox potential, permanganate number, ammonium, nitrate and total nitrogen, total bacterial count at 22° C., total bacterial count at 37° C. and faecal coliform bacteria. The weather, number of swimmers and the chlorine gas addition were continuously registered, and the pH was checked a few times at each bath. In bath 1, an indoor pool with aluminium sulphate precipitation about once a week and with sand filters back-washed every 2 days, less than 10 bacteria/ml. were found in all samples. In bath 2, an outdoor pool with aluminium sulphate precipitation twice a week and with sand filters back-washed twice a week, also few bacteria were found. In bath 3, an outdoor pool with only filtering through sand filters back-washed about every 14 days, high bacterial counts were found every day except the first, when the filters had been newly back-washed. In bath 4, an outdoor pool with only filtering through sand filters back-washed about once a week, high bacterial counts were found now and then during the first 4 days when the weather was warm, but few bacteria were found the last 2 days when the weather was cold and windy, and there were few swimmers.Values from different analyses on the same sample showed relatively good correlation between the redox potential and the free available chlorine. In bath 3 both the redox potential and the available chlorine were weakly correlated to the bacterial count, but in bath 4 there was no such correlation. No other factors were well correlated with the bacterial count either.The bacterial counts at 22° and 37° C. were of the same order. No faecal coliforms were ever found. Use of these bacteria as indicator organism in swimming pools is criticized.The method of using certain minimum values of the free available chlorine as guarantee for a satisfactory bacteriological quality of the swimming pool water is also questioned. The degree of purity of the water is fundamentally connected with the disinfecting power of the available chlorine.Use of certain minimum values of the redox potential, according to these investigations, seems to be a method of somewhat greater accuracy. Provided that the methods of precipitation are performed correctly and filters are being back-washed often enough, then an automatically registering redox potential device, perhaps connected to the chlorine gas pump, ought to constitute a good control of the hygienic quality of a swimming-pool water. This must, however, always be completed by bacteriological examinations, preferably made at high bathing load.

scholarly journals Relationship between Oral Bacterial Count and Postoperative Complications among Patients with Cardiovascular Disease Treated by Surgery: A Retrospective Cohort Study

Healthcare ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 850
Author(s):  
Rie Osako ◽  
Yuhei Matsuda ◽  
Chieko Itohara ◽  
Yuka Sukegawa-Takahashi ◽  
Shintaro Sukegawa ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Postoperative Complications ◽  
Operation Time ◽  
Bacterial Count ◽  
Cardiovascular Complications ◽  
Pericardial Fluid ◽  
Bacterial Counts ◽  
Lung Torsion ◽  
Propensity Score Adjustment ◽  
The Relationship

In this retrospective observational study, we evaluated the relationship between perioperative oral bacterial counts and postoperative complications in cardiovascular disease (CVD) patients. From April 2012 to December 2018, all patients scheduled for surgery received perioperative oral management (POM) by oral specialists at a single center. Tongue dorsum bacterial counts were measured on the pre-hospitalization day, preoperatively, and postoperatively. Background data were collected retrospectively. Among the 470 consecutive patients, the postoperative complication incidence rate was 10.4% (pericardial fluid storage, n = 21; postoperative pneumonia, n = 13; surgical site infection, n = 9; mediastinitis, n = 2; and seroma, postoperative infective endocarditis, lung torsion, and pericardial effusion, n = 1 each). Oral bacterial counts were significantly higher in the pre-hospitalization than in the pre- and postoperative samples (p < 0.05). Sex, cerebrovascular disease, and operation time differed significantly between complications and no-complications groups (p < 0.05). Multivariate analysis with propensity score adjustment showed a significant association between postoperative oral bacterial count and postoperative complications (odds ratio 1.26; 95% confidence interval, 1.00–1.60; p = 0.05). Since the development of cardiovascular complications is a multifactorial process, the present study cannot show that POM reduces complications but indicates POM may prevent complications in CVD patients.

A New Screening Approach For Fast And Accurate Prediction Of Positive And Negative Urine Cultures By SediMAX Compared With The Standard Urine Culture

2021 ◽  
Vol 1 (1) ◽  
pp. 46-55
Author(s):  
Massimo Pieri ◽  
Flaminia Tomassetti ◽  
Paola Cerini ◽  
Roberta Felicetti ◽  
Lucia Ceccaroni ◽  
...  
Keyword(s):  
Blood Cell ◽  
Bacterial Infections ◽  
Urine Culture ◽  
Screening Method ◽  
Significant Proportion ◽  
Bacterial Count ◽  
Urine Samples ◽  
Screening Methods ◽  
Urine Sediment ◽  
Tract Infections

Urinary tract infections (UTI) are the most frequent bacterial infections, and the detection of infection in urine samples is expensive and time-consuming. Also, in laboratories a significant proportion of samples processed yield negative results. For this, screening methods represent an important improvement towards the final UTI diagnosis. SediMAX is an automated microscopy, easier to use in laboratories due to its basic procedure and it is widely used for urine sediment analysis. In our study, we evaluated the performance of SediMAX, applying some screening parameters, compared with the gold standard methods, urine culture, to identify all the positive cases for UTI. We analysed 1185 urine samples from our daily laboratory routine. The basis of our screening model was to establish a cut-off for bacterial count (BACT), as 300 bacteria/µL in order to avoid missing positive cases. However, the sensitivity and the specificity achieved were not enough to identify all UTI infection in urine samples. So, in addition to BACT we have considered other parameters, such as White Blood Cell (WBC), Red Blood Cell (RBC), Yeasts (YEST), Age and Nitrates (NIT). The second screening method reached a sensitivity of 100%, that could be reliably employed in detect of UTIs.

scholarly journals Validation of Questionnaire Methods to Quantify Recreational Water Ingestion

2018 ◽  
Vol 15 (11) ◽  
pp. 2435 ◽  
Author(s):  
Laura Suppes ◽  
Kacey Ernst ◽  
Leif Abrell ◽  
Kelly Reynolds
Keyword(s):  
Cyanuric Acid ◽  
Swimming Pool ◽  
Recreational Water ◽  
Tracer Method ◽  
Response Options ◽  
Water Ingestion ◽  
Pool Water ◽  
Chemical Tracer ◽  
Swimming Pool Water ◽  
Validation Of Questionnaire

Swimming pool water ingestion volumes are necessary for assessing infection risk from swimming. Pool water ingestion volumes can be estimated by questionnaire or measuring a chemical tracer in swimmer urine. Questionnaires are often preferred to the chemical tracer method because surveys are less time consuming, but no research exists validating questionnaires accurately quantify pool water ingestion volumes. The objective of this study was to explore if questionnaires are a reliable tool for collecting pool water ingestion volumes. A questionnaire was issued at four pool sites in Tucson, Arizona to 46 swimmers who also submitted a urine sample for analyzing cyanuric acid, a chemical tracer. Perceived ingestion volumes reported on the questionnaire were compared with pool water ingestion volumes, quantified by analyzing cyanuric acid in swimmer urine. Swimmers were asked if they swallowed (1) no water or only a few drops, (2) one to two mouthfuls, (3) three to five mouthfuls, or (4) six to eight mouthfuls. One mouthful is the equivalent of 27 mL of water. The majority (81%) of swimmers ingested <27 mL of pool water but reported ingesting >27 mL (“one mouthful”) on the questionnaire. More than half (52%) of swimmers overestimated their ingestion volume. These findings suggest swimmers are over-estimating pool water ingestion because they perceive one mouthful is <27 mL. The questionnaire did not reliably collect pool water ingestion volumes and should be improved for future exposure assessment studies. Images of the ingestion volume categories should be included on the questionnaire to help swimmers visualize the response options.

Preslaughter Hide Status of Cattle and the Microbiology of Carcasses

1997 ◽  
Vol 60 (12) ◽  
pp. 1502-1508 ◽  
Author(s):  
JOYCE VAN DONKERSGOED ◽  
KLAUS W. F. JERICHO ◽  
HEIDI GROGAN ◽  
BEN THORLAKSON
Keyword(s):  
Bacterial Count ◽  
Control Points ◽  
Bacterial Counts ◽  
Negatively Associated ◽  
Surface Wetness ◽  
E Coli ◽  
Line Speed ◽  
Critical Control Points ◽  
Plant Personnel ◽  
The Individual

An assessment was made of the association between tag (mud, bedding, and manure) attached to hides of beef cattle at slaughter and bacterial deposition on carcasses. A total of 624 carcasses from 52 lots of cattle in southern Alberta from January to June 1996 were studied at a high-line-speed abattoir (HLSP) which processed 285 carcasses per h and at a slow-line-speed abattoir (SLSP) which processed 135 carcasses per h. Tag was quantitatively assessed on the belly, legs, and sides of 12 carcasses per lot by the same project worker (lot tag score) and for each incoming lot of cattle by plant personnel (plant lot tag score). Swabs (approximately 10 by 10 cm) were taken from the medial rump and sacrum immediately after hide removal and from the brisket and top of shoulder after carcass splitting. These samples were pooled for each carcass and aerobic mesophilic bacteria, coliforms, and Escherichia coli were enumerated. The lot bacterial count was calculated by averaging the individual bacterial results of the 12 carcasses in a lot. At the HLSP, the lot side scores and the plant lot tag scores were negatively associated (P &lt; 0.05) with the aerobic bacteria, coliforms, and E. coli. Counts were lower when tag was shaven off of the hides or when the line speed was slowed, but the reductions in counts were less than 0.5 log10/cm2. At the SLSP, the lot belly score was negatively associated (P &lt; 0.003) with the aerobic bacterial counts. Neither the lot tag score nor the plant lot tag score were associated (P &gt; 0.05) with the bacterial counts. Surface wetness of the hides was weakly (P &lt; 0.05) associated with coli forms and E. coli counts. This study indicates that there is no consistent association between lot tag scores, plant lot tag scores, and bacterial contamination of carcasses. Changes in bacterial counts when associated with lot tag scores, plant lot tag scores, surface wetness of hides, line speed, or shaving off of tag were generally less than 0.5 log10/cm2. Thus, these variables are individually assessed as control points, but not critical control points of HACCP plans for the prevailing beef slaughter processes (including line speed adjustment at the HLSP) at the two plants studied.

scholarly journals Isolation of Bacterial Pathogens from Borehole Water Sources within the University of Port Harcourt

2020 ◽  
pp. 39-44
Author(s):  
H. O. Stanley ◽  
C. N. Eze
Keyword(s):  
Water Samples ◽  
Bacterial Count ◽  
Water Sources ◽  
World Health ◽  
Coliform Bacteria ◽  
Adequate Treatment ◽  
Post Graduate ◽  
Port Harcourt ◽  
Borehole Water ◽  
The University

A study about the bacteriological and physicochemical quality of borehole water at the University of Port Harcourt was carried out. Eight water samples was pooled from NUH Block B (Under graduate Hostel), Nelson Mandela Block B (Undergraduate Hostel), Intercontinental Hostel (Post Graduate Hostel), Donald Ekong Block C (Post graduate Hostel), Clinical Hostel, Staff quarters (Opposite Uniport Bottling plant), Dan Etete (Undergraduate Hostel) and Gambiama Staff quarters designated as Sample 1 to sample 8 respectively. Physical examination was conducted to as the sanitary and hygiene practices within the collection area. It was observed that the undergraduate hostels had the least sanitary practice. Total counts of heterotrophic bacteria count showed that the highest bacterial count was recorded from Sample 2 with bacterial count of 2.3x104 CFU/ml while the least bacterial count was recorded from sample 8 with bacterial count of 3.0x102 CFU/ml. The isolated bacterial species from the water sources were identified as Bacillus sp., Micrococcus sp., E. coli., Serratia sp., Staphylococcus sp., Enterobacter sp., Citrobacter sp. The presence of coliform bacteria in the sampled water source does not comply with the World Health Organization (WHO) standard for coliform bacteria of zero total coliform per 100 ml of water. The borehole water samples collected had pH values within 5.9-6.85 which does not comply with the WHO recommended range for drinking water standards which should fall between ≥7 to ≤9.2. Nitrate concentration as observed amongst the eight water samples was below the WHO standard of 50mg/l. The Total dissolved solutes was below 0.01 in all the tested waters samples. This study has revealed that borehole water from sampled sources within the University of Port Harcourt is not fit for human consumption without adequate treatment.

scholarly journals Removal of Cryptosporidium and polystyrene microspheres from swimming pool water with sand, cartridge, and precoat filters

2011 ◽  
Vol 10 (1) ◽  
pp. 31-42 ◽  
Author(s):  
James E. Amburgey ◽  
Kimberly J. Walsh ◽  
Roy R. Fielding ◽  
Michael J. Arrowood
Keyword(s):  
Tap Water ◽  
Disease Outbreaks ◽  
Field Trials ◽  
Swimming Pool ◽  
Future Research ◽  
Recreational Water ◽  
Polystyrene Microspheres ◽  
Pool Water ◽  
Cryptosporidium Oocysts ◽  
Removal Efficiencies

Cryptosporidium has caused the majority of waterborne disease outbreaks in treated recreational water venues in the USA for many years running. This research project evaluated some common US swimming pool filters for removing Cryptosporidium oocysts, 5-µm diameter polystyrene microspheres, and 1-µm diameter polystyrene microspheres. A 946 L hot tub with interchangeable sand, cartridge, and precoat filters was used at room temperature for this research. Simulated pool water for each experiment was created from Charlotte, NC (USA) tap water supplemented with alkalinity, hardness, chlorine, and a mixture of artificial sweat and urine. Precoat (i.e., diatomaceous earth and perlite) filters demonstrated pathogen removal efficiencies of 2.3 to 4.4 log (or 99.4–99.996%). However, sand and cartridge filters had average Cryptosporidium removals of 0.19 log (36%) or less. The combined low filter removal efficiencies of sand and cartridge filters along with the chlorine-resistant properties of Cryptosporidium oocysts could indicate a regulatory gap warranting further attention and having significant implications on the protection of public health in recreational water facilities. The 5-µm microspheres were a good surrogate for Cryptosporidium oocysts in this study and hold promise for use in future research projects, field trials, and/or product testing on swimming pool filters.

Bacteriophage Therapy of Chronic Nonhealing Wound: Clinical Study

2019 ◽  
Vol 18 (2) ◽  
pp. 171-175 ◽  
Author(s):  
Pooja Gupta ◽  
Hari Shankar Singh ◽  
Vijay K. Shukla ◽  
Gopal Nath ◽  
Satyanam Kumar Bhartiya
Keyword(s):  
Wound Healing ◽  
Biofilm Formation ◽  
Bacterial Infections ◽  
Chronic Wounds ◽  
Bacterial Count ◽  
Complete Healing ◽  
Resistant Bacteria ◽  
Bacteriophage Therapy ◽  
Inflammatory Phase ◽  
Drug Resistant Bacteria

Background: A chronic wound usually results due to halt in the inflammatory phase of wound healing. Bacterial infections and biofilm formation are considered to be the basic cause of it. Chronic wounds significantly impair the quality of life. Antibiotics are now failing due to biofilm formation emergence of drug-resistant bacteria. Objective: This study aims to see the effect of bacteriophage therapy in chronic nonhealing wound infected with the following bacteria: Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. Subject: Patients with chronic nonhealing wound not responding to conventional local debridement and antibiotic therapy were included in the study. The age of patients ranged between 12 and 60 years. Method: A total of 20 patients selected and tissue biopsies and wound swabs were taken for isolation of the bacteria. After confirmation of organism, a cocktail of customized bacteriophages was topically applied over the wound on alternate days till the wound surface became microbiologically sterile. Mean bacterial count and clinical assessment were done and compared at the time of presentation and after 3 and 5 doses of application. Results: A significant improvement was observed in the wound healing, and there were no signs of infection clinically and microbiologically after 3 to 5 doses of topical bacteriophage therapy. Seven patients achieved complete healing on day21 during follow up while in others healthy margins and healthy granulation tissue were observed. Conclusion: Topical bacteriophage application may be quite effective therapy for the treatment of chronic nonhealing wounds.

Laboratory testing protocol to identify critical factors in bacterial compliance monitoring

2003 ◽  
Vol 47 (3) ◽  
pp. 131-136
Author(s):  
M. Abbaszadegan ◽  
P. Ghatpande ◽  
J. Brereton ◽  
A. Alum ◽  
R. Narasimhan
Keyword(s):  
Distribution System ◽  
Membrane Filtration ◽  
Bacterial Count ◽  
The Other ◽  
Residual Chlorine ◽  
First Flush ◽  
Sample Collection ◽  
Service Line ◽  
Bacterial Counts ◽  
Representative Sampling

This research focused on providing guidelines for water utilities on the collection and handling of routine bacteriological samples and in developing scientifically-based approaches in selecting the most representative sampling locations. A laboratory-scale pilot distribution system was designed comprising two parallel loops, one using unlined cast-iron pipe and one using PVC pipe. Each loop contained six sampling ports, including (1) a distribution main dead end faucet, (2) one long (5.5 m; 18 feet) and (3) one short (0.3 m; 1 foot) household copper service line with threaded hose-bibb taps, (4) one hose-bibb with welded faucet, (5) one dedicated sampling port (modeled after a manufacturer’s specifications) and (6) one laboratory-style (PVC) stop-cock sampling port. Residual chlorine concentrations were maintained at 0, 0.5, 1.5 and 2.0 mg/L stages during the course of the experiment. Bacterial samples were collected from the different sampling ports and assayed by membrane filtration and/or spread plate. Nutrient and R2A agars were used for heterotrophic plate counts (HPC), m-Endo agar for total coliform (TC) counts and Chromocult agar for injured bacterial analyses. Several methods of sample collection were tested using various combinations of flushing and tap disinfection, including “first flush” (no flushing, without tap disinfection), flushing only, tap disinfection only (using alcohol or hypochlorite solution) and flushing coupled with tap disinfection. The results indicated that the bacterial counts in samples drawn from dead ends were not significantly different from counts in samples from the other sample port configurations. First flush samples consistently produced the highest bacterial count results. Bacterial counts in samples from the long household copper service line were typically three orders of magnitude higher than in samples from the other sample ports. Thus, there is evidence that long copper household service connections may be unsuitable sample tap configurations for collecting samples intended to represent microbial quality in the distribution system.

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