HEPATOPROTECTIVE ACTIVITY OF WHOLE FLOWER OF HIBISCUS ROSA-SINENSIS LINN EXTRACTS IN WISTAR RATS

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (05) ◽  
pp. 65-70
Author(s):  
Krishna Kumar Agrawal ◽  
Keyword(s):  
Carbon Tetrachloride ◽  
Biochemical Markers ◽  
Hepatoprotective Activity ◽  
Hepatic Damage ◽  
Control Group ◽  
Dependent Manner ◽  
Aqueous Extracts ◽  
Sleeping Time ◽  
Hibiscus Rosa Sinensis ◽  
And Control

The aim of the present study was to evaluate the hepatoprotective effect of ethanolic and aqueous extracts of Hibiscus rosa-sinensis Linn flowers..Carbon tetrachloride-induced hepatic injury in rats and pentobarbitone induced hypnosis methods were used to evaluate the hepatoprotective activity. Alteration in biochemical parameters of hepatic damage, such as alanine transminase (ALT), aspartate transminase (AST), alkaline phosphatase (ALP), triglycerides (TG), cholesterol, total bilirubin, direct bilirubin and pentobarbitone induced sleeping time were tested in different groups of study. Carbon tetrachloride (1mL/kg i.p.) enhances the level of biochemical markers of hepatic damage and increases the sleeping time in mice. Treatment with ethanolic and aqueous extracts of H. rosa-sinensis flowers (200mg/kg and 400mg/kg) has significantly brought back the altered levels of biochemical markers to near normal levels in a dose dependent manner, as compared to silyamarin (100mg/kg) and control group (1% w/V CMC).The result indicaes that the hepatoprotective activity shown may be due to the presence of flavonoids, mucilage, tannins or alkaloids, which justify its folkloric use.

scholarly journals A study to assess the effectiveness of honey dates amla mix on biochemical markers among adolescent girls with Iron deficiency anaemia

2020 ◽  
Vol 11 (2) ◽  
pp. 2288-2293
Author(s):  
Bhuvaneswari G.
Keyword(s):  
Iron Deficiency ◽  
Adolescent Girls ◽  
Biochemical Markers ◽  
Serum Iron ◽  
Iron Deficiency Anaemia ◽  
Control Group ◽  
Deficiency Anaemia ◽  
Post Test ◽  
And Control ◽  
Experimental Group

WHO Global Database on Anaemia for 1993-2005, covering almost half of the world’s population, estimated the prevalence of anaemia worldwide at 25 per cent.The aim of the study is to determine the effectiveness of honey dates amla mix on biochemical markers among adolescent girls with Iron deficiency anaemia. Quantitative experimental and control group pre and post-test design was used in this study. The study was conducted among adolescent girls in two different residential homes. Total study population is 170 adolescent girls were selected by simple random sampling technique. 85 adolescents girls were assigned to the experimental group and 85 participants in control group. For experimental group honey dates amla mix was given for 3 months whereas in  the control group iron and folic acid supplementation were provided. The study results shows that pre and post-test intervention scores of control and experimental group were compared by Wilcoxon scores rank test. Comparison of pre-test and post-test scores of severity of anemia, clinical variables, and level of fatigue and level of satisfaction, bio chemical parameters (reticulocytes, MCV, haematocrite, serum iron, serum ferritin, TIBC) were assessed in both experimental and control group. Between the experimental and control group (unpaired‘t’ test) reticulocytes, mcv, heamatocrite, serum iron serum ferritin showed significant changes in the pre test and post-test but there is no changes in TIBC. The study concluded that alternative nutritional therapy was effective for enhancing the blood heamoglobin & biochemical paramteres level, decreased the clinical variables and fatigue level among the adolescent girls with iron deficiency anemia.

HEPATOPROTECTIVE ACTIVITY OF AERIAL PARTS OF NELUMBO NUCIFERA GAERTN. IN PARACETAMOL AND ISONIAZID-RIFAMPICINE INDUCED HEPATOTOXICITY IN RATS

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (12) ◽  
pp. 47-53
Author(s):  
D Gupta ◽  
Keyword(s):  
Synergistic Effect ◽  
Group Treatment ◽  
Hepatoprotective Activity ◽  
Nelumbo Nucifera ◽  
Control Group ◽  
Aqueous Extracts ◽  
Aerial Parts ◽  
Dose Dependent

Hepatoprotective activity of ethanolic and aqueous extracts (200 and 400 mg/kg b.w.) of Nelumbo nucifera Gaertn. (NNE and NNA respectively) was evaluated by paracetamol and Isoniazid-rifampicine (INH-RMP) induced hepatotoxicity models. In paracetamol induced hepatoxicity model, NNA 400 and NNE 400 produced significant (p<0.005) reduction in SGOT level when compared to that of control group. Treatment with NNE 200, NNE 400 and NNA 400 produced significant reduction (p<0.01) in SGPT levels when compared to that of control group. SALP levels were significantly (p<0.001) reduced in animals treated with Silymarin (standard), ethanolic and aqueous extracts at both doses when compared to that of control. In INH-RMP induced hepatotoxicity model, significant (p<0.001) reduction in SGOT levels was produced in animals treated with NNA 200 and 400, NNE 200 and 400, when compared to control group animals. Ethanolic and aqueous extracts of Nelumbo nucifera at both doses produced significant (p<0.05 and 0.001 respectively) decrease in the SGPT and SALP levels when compared with control group. Hepatoprotective activity was found to be dose dependent. Significant hepatoprotective activity produced by ethanolic & aqueous extracts of Nelumbo nucifera may be a consequence of synergistic effect of the constituents present in the extracts namely, β-sitosterol and flavonoids (kaempferol, quercetin etc.) The results suggest that the ethanolic and aqueous extracts (NNE and NNA) of Nelumbo nucifera aerial parts possess significant hepatoprotective activity.

Evaluation of Hepatoprotective activity of Methanol extract of Persicaria hydropiper: An in vivo screening

2021 ◽  
pp. 5819-5824
Author(s):  
Pooja Kamra ◽  
Mahaveer Singh ◽  
Hardarshan Singh Lamba ◽  
Birendra Srivastava
Keyword(s):  
Carbon Tetrachloride ◽  
Intraperitoneal Administration ◽  
Hepatoprotective Activity ◽  
Dependent Manner ◽  
Standard Drug ◽  
Whole Plant ◽  
Dose Dependent ◽  
Histopathological Investigation ◽  
Different Doses

The present study aimed to evaluate the hepatoprotective potential of methanolic whole plant extract of Persicaria hydropiper in carbon tetrachloride (CCl4) induced hepatotoxicity model. Hepatotoxicity was induced in rats by intraperitoneal administration of carbon tetrachloride (CCl4) for seven days. The extract was thereafter administered at two different doses of 200 mg/kg and 400 mg/kg body weight for next seven days. Silymarin was used as a reference standard. The extract revealed hepatoprotective activity in dose dependent manner. The dose of 400 mg/kg exhibited maximum hepatoprotective ability as apparent from several evaluation parameters including liver function profile, bilirubin, antioxidant enzymes as well as histopathological investigation which was comparable to the standard drug Silymarin respectively. These findings sustenance the use of the extract as an adjuvant with existing therapy for treatment of liver ailments.

scholarly journals Pharmacoprophylaxis of liver diseases: creating a new hepatoprotector

2020 ◽  
Vol 17 ◽  
pp. 00061
Author(s):  
Svetlana Zykova ◽  
Sergey Shurov ◽  
Aleksey Savinkov ◽  
Nino Gugushvili ◽  
Vladimir Talismanov
Keyword(s):  
Oxidative Stress ◽  
Carbon Tetrachloride ◽  
Toxic Effect ◽  
Alanine Aminotransferase ◽  
Liver Diseases ◽  
Hepatoprotective Activity ◽  
Control Group ◽  
Biochemical Indicators ◽  
Intact Group ◽  
Biochemical Studies

The article presents a study of the hepatoprotective activity of a tricyclic heterocycle, which refers to 5, 6, 7, 8-tetrahydroquinolines. The effect of 8, 8-dimethyl-5-p-tolyl-8, 9-dihydro-2H-pyrido [4, 3, 2-de] cinnolin-3 (7H) was studied on rats under the influence of the model of toxic hepatosis induced by carbon tetrachloride to find out the indicators of peroxidation and biochemical indicators. Biochemical studies have shown that modelling toxic fat hepatosis caused by the inception of carbon tetrachloride to rats increased the activity of alanine aminotransferase by 2.5 times more compared with the intact group, indicating the development of oxidative stress induced by the treatment of pyrido [4, 3, 2] Cinnol I that reduced the toxic effect of CTC by 79.9 %. Mexidol had a less pronounced hepatoprotective effect: the activity of Alanine aminotransferase on animals of the second group was lower by 29.2 % than on rats from the control group. Thus, a new compound with hepatoprotective activity has been developed and studied.

scholarly journals Study of some toxicological aspects of Alfa-cypermethrin in rabbits

2010 ◽  
Vol 9 (3) ◽  
pp. 82
Author(s):  
Dh. R.H. Al-Fetly And N. K. M. Al-Nakeeb
Keyword(s):  
Biochemical Study ◽  
Liver Parenchyma ◽  
Blood Parameters ◽  
Bile Secretion ◽  
Control Group ◽  
Histopathological Study ◽  
Dependent Manner ◽  
Petechial Hemorrhage ◽  
Hb Concentration ◽  
And Control

This study was conducted to evaluate the immediate alterations that occur in rabbitsafter acute toxicity with single oral dose of cypermethrin. Twenty one rabbits were randomlydivided into three equal groups: treated groups (A1), (A2) and control group used in thisstudy. Groups (A1), (A2) received orally cypermethrin in dose of (25, 50 mg/kg body weight)respectively, While control group received normal saline at same time. Most obvious clinicalsings in the treated groups were Restlessness, salivation, licking of legs and face, frequenturination, muscular tremor, incoordination, and ataxia appeared in dose dependent manner. Inaddition, significantly increased in respiratory rate and heart rate in treated groups comparedwith control group and appeared petechial hemorrhage and congestion in the conjunctiva intreated groups. The blood samples were taking to evaluate some of blood parameters includes(RBC counts, WBC counts, PLT counts, Hb concentration, PCV, RBCs indices (MCV, MCH,MCHC, RDW -CV and RDW - SD), PDW, MPV, P-LCR and some biochemical study asALT and AST. Two rabbits from each group were killed to evaluate the histopathologicalalteration in liver. The statistical analysis revealed that there were significant decreased inRBC counts and Hb concentration (P<0.05) in treated groups (A1), (A2) as compared withcontrol group. The PCV percentage, platelet counts and MCHC values revealed significantdecreased (P<0.05) between group (A2) and control group. The RDW-SD, RDW-CV valveswere significantly increased (p<0.05) between group (A1) and control group. Other parameterstudied did not show significant differences between treated groups and control group. Thestatistical analysis revealed that the levels of ALT and AST were significantly elevated(P<0.05) in (A1 and A2) groups as compared with control group. In gross appearance therewere petechial hemorrhage and erosion of liver parenchyma. Histopathological study revealedthat there were engorgement of hepatic vein with presence of extravasated RBCs between thehepatocytes in group (A1), while group (A2) the hepatocytes undergo atrophy and severenecrosis and destruction with infiltration of polymorphnuclear leukocytes and engorgement ofsome portal trait with bile secretion

scholarly journals Comparison of biomarkers between genotypes 1a and 3a in hepatitis C virus patients with control group

2019 ◽  
Vol 6 (4) ◽  
pp. 3121-3130
Author(s):  
Sajjad Yazdansetad ◽  
Hadi Razavi Nikoo ◽  
Seyed Mahmoud Azimi ◽  
Alireza Mohebbi ◽  
Massumeh Niazi ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Biochemical Markers ◽  
Liver Enzymes ◽  
Control Group ◽  
Genotype 1A ◽  
Significant Difference ◽  
Hcv Genotyping ◽  
And Control ◽  
Genotype 3A

Introduction: Three percent of people worldwide are infected with Hepatitis C virus (HCV). A few studies have been performed to evaluate the biochemical markers of the disease. In the current study, biochemical markers were evaluated in HCV patients and the control group. Methods: Two sex- and age-matched healthy individuals (n = 100) and HCV positive patients (n = 100) were included (mean age of 20-75, 26.0% females and 74.0% males). Biochemical markers, including liver enzymes (ALT, AST and ALP), lipid profiles (cholesterol, LDL, and HDL) and triglyceride (TG) were investigated in both groups. HCV genotyping was also performed by Polymerase Chain Reaction (PCR) and OHNO methods. Results: The biochemical markers between HCV patients and controls were compared (cholesterol, ALP, AST, ALT, LDL: p = 0.0001, HDL: p = 0.002, TG: p = 0.003), and statistically significant difference was found between two groups. The biochemical markers between HCV patients and the control group in terms of age was compared and no differences was observed (p = 0.741), however, there was a significant difference in sex between HCV patients and control group (26.0% females, 74.0% males in control group, and x% females and y% males in HCV patients) (p = 0.032). The results of HCV genotyping showed that 39 patients were genotype 1a, and 43 patients were genotype 3a, and 1 patient was genotype 2a. Evaluation of biochemical markers in patients with genotype 1a and 3a showed that there were significant differences in cholesterol (p = 0.001), LDL (p = 0.001) and HDL (p= 0.003) levels, but there were no significant differences in liver enzymes and TG levels in both genotypes. Conclusion: In the present study, we found significant difference in biochemical markers between HCV patients and controls. In HCV patients, the biochemical markers were dependent on HCV genotypes, and their levels in genotype 1a were higher than genotype 3a. In conclusion, biochemical markers are one of the most important factors for the identification of treatment.

scholarly journals COMBINATION OF MOMORDICA CHARANTIA AND PHYLLANTHUS AMARUS FOR HEPATOPROTECTIVE ACTIVITY IN ETHANOL AND ANTI-TUBERCULAR DRUGS INDUCED HEPATOTOXICITY IN RATS

2020 ◽  
pp. 166-171
Author(s):  
PURNIMA ASHOK ◽  
LAVANYA P. ◽  
HEMANTH K. ◽  
SANDESH K. M. ◽  
YUVRAJ SINGH SURANA
Keyword(s):  
Liver Damage ◽  
Biochemical Markers ◽  
Synergistic Effects ◽  
Hepatoprotective Activity ◽  
Momordica Charantia ◽  
Control Group ◽  
Phyllanthus Amarus ◽  
Histopathological Evaluation ◽  
Anti Oxidant ◽  
Oxidant Status

Objective: To evaluate the synergistic protective effect of Momordica charantia and Phyllanthus amarus combination (MC+PA) of doses 200 and 400 mg/kg on the liver in different experimental models of hepatotoxicity. Methods: The hepatoprotective activity was evaluated in ethanol and anti-tubercular drugs (isoniazid-INH, rifampicin-RIF) induced hepatotoxicity models. Hepatotoxicity in both models was induced to all groups except the normal control. Intoxicated rats were treated with silymarin and various doses of MC+PA for 8 d in ethanol-induced and 21 d in INH+RIF induced hepatotoxicity models. At the completion of study, the biochemical markers and the anti-oxidant status (SOD and MDA) were measured and also the histopathological evaluation of the liver tissue was carried out. Results: Combination therapy remarkably reduced the elevated profile of the biochemical markers and thereby improved the anti-oxidant status, thus exhibiting the synergistic hepatoprotective effect when compared with the positive control group (p<0.001). Histopathological evaluation demonstrated that MC+PA decreased the liver damage significantly in comparison with the positive group. Conclusion: The current work suggests that the combined extract showed synergistic effects on ethanol and anti-tubercular drugs induced hepatotoxicity models by significantly decreasing the liver damage.

scholarly journals ANTIOXIDANT PROPERTY OF ANTIHYPERTENSIVE DRUGS (CARVEDILOL, ENALAPRIL, AND AMLODIPINE) ON LIVER FUNCTION IN ANIMAL (RATS) MODELS

2016 ◽  
pp. 292
Author(s):  
Pusuloori Rajesh*
Keyword(s):  
Carbon Tetrachloride ◽  
Biochemical Markers ◽  
Antihypertensive Drugs ◽  
Beta Blockers ◽  
Serum Levels ◽  
Antioxidant Property ◽  
Hepatoprotective Activity ◽  
Albino Rats ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Serum Glutamate Pyruvate Transaminase

<p>ABSTRACT<br />Objective: The antihypertensive drugs (beta-blockers - carvedilol, calcium channel blocker - amlodipine, and angiotensin-converting enzyme<br />inhibitor - enalapril) were investigated for its hepatoprotective effect by antioxidant property against carbon tetrachloride (CCl<br />)-induced acute liver<br />damage on albino rats. <br />Methods: In this study the liver was damaged by giving Carbon tetra chloride (CCl<br />4<br />)-1 ml/kg-p.o. After giving CCl<br /> (1 ml/kg), it significantly elevated<br />the serum levels of biochemical markers such as serum glutamate-pyruvate transaminase, serum glutamic oxaloacetic transaminase, alkaline<br />phosphatase, total bilirubin, protein, and depleted antioxidant enzymes glutathione. After treatment with carvedilol (10 mg/kg), enalapril (0.5 mg/kg),<br />and amlodipine (10 mg/kg) for 2 weeks, these drugs were significantly reduces the elevated levels of biochemical markers mentioned above.<br />Conclusion: These results suggest that these antihypertensive drugs may have the potential therapeutic value in the treatment of CCl<br />4<br />-induced<br />hepatic damage and some liver diseases. Hepatoprotective activity of these drugs may be attributed to the antioxidant principles in it.<br />Keywords: Antioxidant, Carvedilol, Enalapril, Amlodipine, Carbon tetrachloride, Hepatoprotective.<br />4</p><p>4</p>

scholarly journals Evaluation of Hepatoprotective Effect of Leaves ofCassia sopheraLinn.

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Arijit Mondal ◽  
Sanjay Kumar Karan ◽  
Tanushree Singha ◽  
D. Rajalingam ◽  
Tapan Kumar Maity
Keyword(s):  
Carbon Tetrachloride ◽  
Total Protein ◽  
Total Bilirubin ◽  
Serum Alkaline Phosphatase ◽  
Histopathological Examination ◽  
Elevated Serum ◽  
Hepatoprotective Activity ◽  
Hepatic Damage ◽  
Protein Levels ◽  
Serum Glutamate

In the present study, the hepatoprotective activity of ethanolic extracts ofCassia sopheraLinn. leaves was evaluated against carbon-tetrachloride- (CCl4-) induced hepatic damage in rats. The extracts at doses of 200 and 400 mg/kg were administered orally once daily. The hepatoprotection was assessed in terms of reduction in histological damage, changes in serum enzymes, serum glutamate oxaloacetate transaminase (AST), serum glutamate pyruvate transaminase (ALT), serum alkaline phosphatase (ALP), total bilirubin, and total protein levels. The substantially elevated serum enzymatic levels of AST, ALT, ALP, and total bilirubin were restored towards the normalization significantly by the extracts. The decreased serum total protein level was significantly normalized. Silymarin was used as standard reference and exhibited significant hepatoprotective activity against carbon tetrachloride-induced hepatotoxicity in rats. The biochemical observations were supplemented with histopathological examination of rat liver sections. The results of this study strongly indicate thatCassia sopheraleaves have potent hepatoprotective action against carbon tetrachloride-induced hepatic damage in rats. This study suggests that possible activity may be due to the presence of flavonoids in the extracts.

Hepatoprotective and antioxidant activity of hydromethanolic extract of Daniella oliveri leaves in carbon tetrachloride-induced hepatotoxicity in rats

2015 ◽  
Vol 26 (5) ◽  
Author(s):  
Samuel Okwudili Onoja ◽  
Gideon Kelechi Madubuike ◽  
Maxwell Ikechukwu Ezeja
Keyword(s):  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Negative Control Group ◽  
Hepatoprotective Activity ◽  
Negative Control ◽  
Control Group ◽  
Photometric Assay ◽  
Dose Dependent Increase ◽  
Dose Dependent

AbstractThe hepatoprotective activity was investigated using carbon tetrachloride (CClThe pretreatment with extract (100, 200, and 400 mg/kg) and silymarin (100 mg/kg) produced a significant (p<0.05) dose-dependent increase in hepatoprotective activity when compared with the negative control group. The extract (25–400 μg/mL concentration) produced a concentration-dependent increase in antioxidant activity in 2, 2-diphenyl-1-picrylhydrazine (DPPH) photometric assay. The ICThe results of the study suggest that

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