scholarly journals RhC Phenotyping, Adsorption/Elution Test, and SSP-PCR: The Combined Test for D-Elute Phenotype Screening in Thai RhD-Negative Blood Donors

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Songsak Srijinda ◽  
Chamaiporn Suwanasophon ◽  
Unchalee Visawapoka ◽  
Malinee Pongsavee
Keyword(s):  
Blood Group ◽  
Blood Donors ◽  
Blood Type ◽  
Thai Population ◽  
Indirect Antiglobulin Test ◽  
Phenotype Screening ◽  
Combined Test ◽  
Clinically Significant ◽  
D Antigen ◽  
Asian People

The Rhesus (Rh) blood group is the most polymorphic human blood group and it is clinically significant in transfusion medicine. Especially, D antigen is the most important and highly immunogenic antigen. Due to anti-D, it is the cause of the hemolytic disease of the newborn and transfusion reaction. About 0.1%–0.5% of Asian people are RhD-negative, whereas in the Thai population, the RhD-negative blood type only occurs in 0.3%. Approximately 10%–30% of RhD-negative in Eastern Asian people actually were D-elute (DEL) phenotype, the very weak D antigen that cannot be detected by indirect antiglobulin test (IAT). There are many reports about anti-D immunization in RhD-negative recipients through the transfusion of red blood cells from individuals with DEL phenotype. D-elute phenotype screening in Thai RhD-negative blood donors was studied to distinguish true RhD-negative from DEL phenotype. A total of 254 Thai serologically RhD-negative blood donors were tested for RhCE phenotypes and anti-D adsorption/elution test. In addition, RhC(+) samples were tested for RHD 1227A allele by SSP-PCR technique. The RhD-negative phenotype samples consisted of 131 ccee, 4 ccEe, 1 ccEE, 101 Ccee, 16 CCee, and 1 CcEe. The 42 Ccee and 8 CCee phenotype samples were typed as DEL phenotype and 96% of DEL samples were positive for RHD 1227A allele. The incidence of RhC(+) was 46.4%, and 48 of the 118 RhC(+) samples were positive for both anti-D adsorption/elution test and SSP-PCR technique for RHD 1227A allele. The sensitivity and specificity were 96% and 100%, respectively, for RHD 1227A detection as compared with the adsorption/elution test. In conclusion, RhC(+) phenotype can combine with anti-D adsorption/elution test and RHD 1227A allele SSP-PCR technique for distinguishing true RhD-negative from DEL phenotype.

scholarly journals PREVALENCE OF BOMBAY BLOOD GROUP AMONGST THE HEALTHY BLOOD DONORS AT A TERTIARY CARE CENTRE IN WESTERN RAJASTHAN, INDIA

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Kaluram Meghwal ◽  
N.L. Mahawar ◽  
Dev Raj Arya ◽  
Arun Bharti ◽  
Shailendra Vashistha ◽  
...  
Keyword(s):  
Blood Group ◽  
Blood Donors ◽  
Tertiary Care ◽  
Tertiary Care Centre ◽  
Indirect Antiglobulin Test ◽  
Donor Population ◽  
Study Population ◽  
Clinically Significant ◽  
Group B ◽  
Blood Grouping

Introduction: The prevalence of Bombay (Oh Phenotype) is not precisely known in Rajasthan state. Because of the fact that Bombay blood group is clinically significant, we decided to conduct a study at our centre to determine the prevalence of Bombay blood group among blood donor population in Western Rajasthan. Methodology: This blood bank based prospective study was carried out amongst the blood donors over a period of 11 months, i.e., from February 2019 to December 2019. Total 30,000 donor samples were screened for ABO-RhD blood grouping and antibody screening. Auto control, indirect antiglobulin test (IAT) and Bombay blood group (Anti-H lectin) tests were run on all the O blood group samples showing agglutination with O reagent cells (in reverse grouping). Donors negative on Anti-H testing were tested for saliva A, B and H antigens and interpreted. Results: Out of the total 30,000 donors, the maximum number of donors had blood group B+ (32.76%), followed by O+ (29.9%), A+ (20.41%), AB+ (8.06%), B- (3.11), O- (3.02%), A- (1.93%) and AB- (0.8%). Prevalence of Bombay blood group among study population was calculated to be 0.003%. Conclusion: Bombay phenotype is a rare blood group but not uncommon. If serum grouping is not performed, it is misdiagnosed as blood group O. Therefore both forward and reverse blood grouping should be done on 100% samples. Keywords: Bombay, Anti-H lectin, Serum grouping, Blood donors.

scholarly journals Urgent Transfusion in a New Patient with Rare Blood Type

Blood ◽  
2020 ◽  
Vol 136 (Supplement 1) ◽  
pp. 28-28
Author(s):  
Catherine Dubé ◽  
Scherzinger Kritikopoulos ◽  
Marsha Downie ◽  
Elizabeth Krok ◽  
Katerina Pavenski
Keyword(s):  
B Cells ◽  
Blood Group ◽  
Research Funding ◽  
Antigen Expression ◽  
Cord Compression ◽  
Positive Control ◽  
Blood Type ◽  
Patient Specimen ◽  
Field Reaction ◽  
Clinically Significant

Background/Case Studies: Bombay is a rare blood group characterized by the absence of H substance at the surface of RBCs leading to naturally occurring anti-H antibodies. Anti-H presents the risk of severe hemolytic transfusion reactions in these patients. The case presented is of a 32-year-old female of Middle Eastern origin, who presented with a traumatic vertebral fracture with spinal cord compression and required urgent neurosurgery. Her presenting hemoglobin was 84 g/L. She had no previous group and screen on record, had never been transfused and had a history of a remote miscarriage. Study Design/Methods: Forward blood group with an automated gel-method instrument revealed the following reactions: negative with anti-A, unable to interpret (?) with anti-B, 4+ with anti-D. Reverse grouping revealed the following reactions: 4+ with A1-cells and an unexpected 1+ with B-cells. The antibody screen and 11 cell panel in gel (Micro Typing Systems) 2+; the panel, with enhancement reacted 3+ in Ficin. The auto control was negative. A second panel and pre-warm panel produced the same findings. An antibody reacting at 37C against a high-frequency antigen was suspected. Patient specimen was sent for investigation to the reference lab (RL), which performed blood group by manual tube test, antibody identification with panels by manual tube PEG-IAT method; RL also sent a sample for ABH sequencing (Sanger). Results/Findings: A thawed frozen plasma sample from a previous Bombay patient 12 years prior showed no reactivity against the patient's RBCs; positive control included. A frozen Bombay RBC unit was ordered urgently from the blood supplier and was crossmatch compatible. The patient underwent surgery and was transfused with a single unit of RBC for peri-operative bleeding. She was treated with erythropoietin and IV iron post-operatively and did not require any further transfusions. The investigation at the RL showed mixed field reaction on forward blood typing with anti-B and anti-A,B and negative reaction with anti-A commercial reagents. The RL reverse grouping showed 4+ with all O H+ and A1 red cells, but 2+ with B cells. The autocontrol and group O H- cells did not react, confirming anti-H and suggesting Para-Bombay group. ABH sequencing revealed a normal B allele (ABO*B.01) while genotyping of FUT1 revealed a null allele (FUT1*01N.12) and weak H allele (FUT1*01W.23). FUT2 genotyping (FUT2*01N.02) predicted a nonsecretor (sese) phenotype. Conclusions: This patient with non-secretor status, variant H production, clinically significant anti-H, greatly reduced B antigen expression, should be treated as a Bombay (Oh) for transfusion purposes. She was counselled and provided with an antibody card and a letter. This case illustrates the importance of timely communication with the clinical team about the risks and benefits of transfusion pending antibody identification, as it could have proved fatal in this case. Figure Disclosures Pavenski: Bioverativ:Research Funding;Shire/Takeda:Honoraria;Octapharma:Research Funding;Alexion:Honoraria, Research Funding;Sanofi:Research Funding;Ablynx/Sanofi:Honoraria, Research Funding.

scholarly journals Prevalence of ABO, RhD and other clinically significant Blood Group Antigens among blood donors at tertiary care center, Gwalior

2020 ◽  
Vol 9 (2) ◽  
pp. 437
Author(s):  
Shelendra Sharma ◽  
Dharmesh Chandra Sharma ◽  
Sunita Rai ◽  
Anita Arya ◽  
Reena Jain ◽  
...  
Keyword(s):  
Blood Group ◽  
Blood Donors ◽  
Care Center ◽  
Tertiary Care ◽  
Blood Group Antigens ◽  
Tertiary Care Center ◽  
Clinically Significant

Frequency of Minor Blood Group Antigens Among Hispanic-American Blood Donors in Southwest Texas.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 4188-4188
Author(s):  
Michael Silvey ◽  
Rachel Beddard ◽  
Yidong Chen ◽  
Melissa Frei-Jones
Keyword(s):  
Blood Group ◽  
Race And Ethnicity ◽  
Retrospective Cohort ◽  
Blood Donors ◽  
Conflicts Of Interest ◽  
South Texas ◽  
Blood Type ◽  
Blood Group Antigens ◽  
Hispanic American ◽  
Antigen Testing

Abstract Abstract 4188 The frequency of minor blood group antigens has not been described among Hispanic blood donors. A retrospective cohort study was performed using the South Texas Blood and Tissue Center (STBTC) donor database, LifeTrak. Blood donors self-identified race and ethnicity. Caucasian and Hispanic donors were eligible. Donors who did not have minor antigen testing performed were not included. The STBTC has a high proportion of Hispanic blood donors (299047/779409, 38%), who are primarily of Mexican ancestry. Minor antigen testing was performed in 10% (74140/779409) of all donors; 8% (25394/299407) of Hispanic donors and 11% (42891/392074) of Caucasian donors. Hispanic donors were significantly more like to express blood type O Rh+ than Caucasian donors (13920/25394, 55% v 14947/74140, 35%; p<0.001). The frequency of ABO and RhD blood groups were similar to those reported previously in Caucasian and Hispanic donors (Garraty, et al. Transfusion, 2004). Of the minor antigens, expression of Rh E also differed significantly between Hispanic and Caucasian donors (4706/11744, 40% v 5416/19497, 28%; p<0.001). The prevalence of the other minor antigens were more similar and are reported in Figure 1. Complete Rh haplotypes were available in 8296 Caucasian donors and 7176 Hispanic donors. The haplotype RhDce differed significantly in 36% of Caucasians and 50% of Hispanics (3022/8296 v 3589/7176). The haplotype RhDce was previously reported as most common in African-Americans at 44% (Avent, et al. Blood, 2000). Haplotype RhDCE was found in 8% of Caucasians and 10% of Hispanics (660/8296 v 643/7176); haplotype RhDcE in 16% of Caucasians and 15% of Hispanics (1354/8296 v 1041/7176); and haplotype RhDCe was found in 39% of Caucasians and 27% of Hispanics (3260/8296 v 1903/7176). Differences exist in the frequency of minor blood group antigens among Caucasian and Hispanic blood donors in Southwest Texas. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Associations between seroprevalence of Helicobacter pylori and ABO/rhesus blood group antigens in healthy blood donors in southwest Iran

2021 ◽  
Vol 49 (12) ◽  
pp. 030006052110588
Author(s):  
Azar Dokht Khosravi ◽  
Mehrandokht Sirous ◽  
Morteza Saki ◽  
Sakineh Seyed-Mohammadi ◽  
Seyed Reza Modares Mousavi ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Blood Group ◽  
Blood Donors ◽  
Blood Groups ◽  
Blood Type ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Group Antigens ◽  
Chi Square ◽  
Cytotoxin Associated Gene A ◽  
H Pylori

Objective To investigate correlations between ABO/rhesus (Rh) blood group antigens and anti- Helicobacter pylori and anti-cytotoxin-associated gene A (CagA) seropositivity in blood donors. Methods A total of 311 blood donors were enrolled. ABO and Rh blood groups were determined using hemagglutination tests. Specific anti- H. pylori IgG and anti-CagA IgG antibodies in sera were quantitated by enzyme-linked immunosorbent assay. Correlations between blood groups and anti- H. pylori and anti-CagA seropositivity were evaluated using the Chi-square test. Results O+ was the most frequent blood type (38%, n = 118). Anti- H. pylori IgG seropositivity was observed in 240 (77.2%) blood donors, while anti-CagA IgG seropositivity was observed in 132 (42.5%) blood donors. Although seropositivity rates for both anti- H. pylori and anti-CagA IgG were higher in individuals with blood type O, no statistically significant associations were observed between seropositivity and any ABO/Rh blood groups. Conclusion Individuals with blood type O may have higher rates of H. pylori seropositivity.

scholarly journals Clinically Significant Minor Blood Group Antigens amongst North Indian Donor Population

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Divjot Singh Lamba ◽  
Ravneet Kaur ◽  
Sabita Basu
Keyword(s):  
Racial Differences ◽  
Blood Group ◽  
Blood Donors ◽  
Blood Group Antigen ◽  
Blood Group System ◽  
Blood Group Antigens ◽  
Young Females ◽  
Group System ◽  
Clinically Significant ◽  
Antigen Frequency

Background. Racial differences in blood group antigen distribution are common and may result in striking and interesting findings. These differences in blood group antigen distribution are important due to their influence on the clinical practice of transfusion medicine.Study Design and Methods. This is a prospective study, involving 1000 healthy regular repeat voluntary blood donors associated with the department. The clinically significant minor blood group antigens of these donors were studied.Results. Out of 1000 healthy regular repeat voluntary blood donors, 93% were D positive and 2.8% were K positive. Amongst the Rh antigens, e was the most common (99%), followed by D (93%), C (85.1%), c (62.3%), and E (21.5%). Within the MNS blood group system, antigen frequency was M (88%), N (57.5%), S (57.8%), and s (87.5%). Within the Duffy blood group system, antigen frequency wasFya(87.3%) andFyb(58.3%).Conclusions. This data base will help us to prevent alloimmunisation in young females, pregnant women, and patients who are expected to require repeated transfusions in life by providing them with antigen matched blood. Antigen negative blood can also be made available without delay to already alloimmunized multitransfused patients.

scholarly journals Prevalence of Lewis Blood Group Polymorphisms in Southern Thai Blood Donors

2021 ◽  
Author(s):  
Natthaphon Nanakorn ◽  
Suwimon Boonthongkhao ◽  
Supattra Mitundee ◽  
Natda Tonwong
Keyword(s):  
Blood Group ◽  
Blood Donors ◽  
Polymerase Chain Reaction Amplification ◽  
Nucleotide Polymorphisms ◽  
Thai Population ◽  
Single Nucleotide ◽  
Lewis Blood Group ◽  
Reaction Amplification ◽  
Polymerase Chain ◽  
Negative Phenotype

Objective: To determine the frequencies of five of the most common (59T>G, 202T>C, 314C>T, 508G>A and 1067T>G) single nucleotide polymorphisms (SNPs) of the FUT3 gene in Thai blood donors and examine their associations with the presence or absence of Lewis antigens on red blood cells.Material and Methods: A total of 364 donor specimens from Songklanagarind Hospital and Regional Blood Centre XII Songkhla, Thailand, were recruited for the study. Molecular analysis of each SNP was performed by polymerase chain reaction amplification with sequence-specific primers (PCR-SSP). The Lewis phenotype was investigated in 159 individuals using the standard hemagglutination test.Results: The frequencies of the SNPs were 32.0% (59T>G), 46.6% (202T>C), 21.7% (314C>T), 37.9% (508G>A), and 25.0% (1067T>A). The prevalences of the Lewis phenotype were 61.0% for Le(a-b+), 7.6% for Le(a+b-), 11.3% for Le(a+b+), and 20.1% for Le(a-b-). The Lewis-negative phenotype was significantly associated with homozygosity in 59T>G and 1067T>A (χ2 =49.873, and χ2 =44.520, respectively).Conclusion: Our findings suggest that le59,1067 is largely responsible for the Lewis-negative phenotype in our southern Thai population. Genetic variations in FUT3 polymorphisms may be used as molecular markers for ethnicity and to help understand the roles of the Lewis blood group in infections or clinical diseases.

scholarly journals Detection of red cell alloantibodies in thalassaemia patients

2020 ◽  
Vol 7 (2) ◽  
pp. 419 ◽  
Author(s):  
Ansuman Sahu ◽  
Pankaj Parida ◽  
Smita Mahapatra ◽  
Binay Bhusan Sahoo
Keyword(s):  
Blood Group ◽  
Blood Donors ◽  
Red Cells ◽  
P Value ◽  
Blood Group Antigens ◽  
Red Cell ◽  
Thalassaemia Major ◽  
Medical College ◽  
Blood Group Serology ◽  
Clinically Significant

Background: β-thalassaemia patients receive regular blood transfusion to thrive. Due to antigen disparity between the blood donors and these patients they develop red cell alloantibodies due to alloimmunization.  The objective of this study is to predict the frequency of red cell alloimmunization amongst β-thalassaemia major patients receiving regular blood transfusion.Methods: This study including 106 patients with β-thalassaemia was conducted in the department of Transfusion Medicine, S. C. B. Medical College, Cuttack for a period of 12 months. Alloantibodies to different red cell blood group antigens in multi-transfused thalassaemia patients were detected using the glass bead technology for blood group serology in the present study.Results: Out of 106 β-thalassaemia major patients included in the study, 7.5% of patients developed alloantibodies, all being clinically significant. The alloantibodies were anti-E, anti c, anti e and anti-D. The rate of incidence of these alloantibodies was 3.8%, 1.9%, 0.9% and 0.9% respectively.  There was a significant association between alloantibody formation with number of transfused packed red cells (Mann-Whitney Test: p value = 0.035) and age at first transfusion (p value = 0.001). The factors having no association with alloimmunization to red cell antigens are age and gender.Conclusions: Alloimmunization to various erythrocyte blood group antigens is a common problem in multi-transfused β-thalassaemia patients. There is an association between number of transfused packed red cells and age at first transfusion with alloantibody formation in the study.

scholarly journals GAMBARAN PENDONOR DARAH DI UTD PMI KABUPATEN BANTUL TAHUN 2020

2021 ◽  
Vol 16 (3) ◽  
pp. 938-943
Author(s):  
Atik Dina Naseha ◽  
Nur’Aini Purnamaningsih ◽  
Kuswanto Hardjo
Keyword(s):  
Blood Group ◽  
Blood Donors ◽  
Blood Donation ◽  
Secondary Data ◽  
Blood Type ◽  
Age Group ◽  
Social Scale ◽  
The Social ◽  
Male Sex ◽  
Activity Report

At the end of 2019, it was found that there was a very fast mutating virus called the Covid-19 virus, this virus causes diseases ranging from coughs to colds to death, efforts to prevent the spread of this virus by limiting the social scale of the community which causes a decrease in the number of blood donors which has an impact on the amount of blood stock in Blood Donation Unit. The objective of this study was to determine the description of blood donors based on blood type, gender, age, and type of donor at Blood Donation Unit Bantul Regency in 2020. This study was an activity report by observing secondary data obtained from Blood Donation Unit Bantul Regency. The number of donors was 8.212 donors, of which the number of donations from inside the building was 5.936 donors (72,28%), and the number of donations from mobile units was 2.276 donors (27,72%). The results showed that the majority of blood donors were blood group 0, which was 3.506 (42,69%), and the least blood type was AB, which was 468 (5.70%). The majority of the gender of the donor was male, namely 7.020 (85,48%), for female, 1.192 (14,52%). The majority of donors are aged 17-24 years, namely 1.738 (21,16%), and at least 65 years old 45 (0,55%). The majority of voluntary donors were 5.151 (62,73%), and the subtitute/ family donors were 1.034 (12,59%). There were 8.212 blood donors where the majority of blood group 0 was 3.506 (42,69%), male sex was 7.020 (85,48%), in the 17-24 year age group 1.738 (21,16%), and repeated voluntary donors were 5.151 (62,73%).

scholarly journals Discrepancies Between SNPs and Serology in Calling Blood Types in Asian and Native American Blood Donors

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 1552-1552
Author(s):  
Jill M Johnsen ◽  
Gayle T Teramura ◽  
Samantha Harris ◽  
Meghan Delaney
Keyword(s):  
Genetic Variation ◽  
Native American ◽  
Ethnic Groups ◽  
Blood Group ◽  
Blood Donors ◽  
Blood Group Antigen ◽  
Blood Type ◽  
Blood Group Antigens ◽  
Gene Variants ◽  
Blood Types

Abstract Introduction: Blood types result from genetic variation at blood group genes which directs the expression of blood group antigens on red blood cells. Determination of blood types is clinically important, as blood type mismatches can instigate potentially life-threatening allo-immune responses in transfusion recipients, transplant patients, and pregnant women. Blood types present in Asian American and Native American populations but rare or absent in the blood donor inventory, which is largely European American, can pose significant challenges to effective transfusion and pre-transfusion testing. We sought to more deeply characterize the blood type diversity present in Asian and Native Americans. Methods: Consenting blood donors self-identified to be of Asian or Native American descent were eligible. Conventional serologic methods were used determine C, Jka, Jkb, M, and N blood types. Genotyping was performed with a blood type SNP array (HEA Beadchip™, BioArray Solutions) to genetically assign c, C, e, E, K, k, Kpa, Kpb, Jsa, Jsb, Jka, Jkb, Fya, Fyb, , M, N, S, s, Lua, Lub, Dia, Dib, Coa, Cob, Doa, Dob, Joa, Hy, LWa, LWb, Sc1, and Sc2. For both methods, blood type was designated as positive “+” (consistent with presence of a blood group antigen) or negative “0” (consistent with absence of a blood group antigen). A SNP-serology discrepancy was defined as a “+” assignment by one method and a “0” by the other. Ambiguous SNP determinations were called “+” or “0” based upon relative signal intensities using established algorithms whenever possible; if not possible, the SNP-determined blood types were designated either inconclusive (IC) or low signal (LS). Results: A total of 8454 Asian and Native American blood donors representing 9 distinct ethnic groups were included in the study. As expected, the frequencies of rare and uncommon blood types differed between ethnic groups and in comparison to Europeans. Overall, 4.7% of blood donors studied exhibited one or more SNP-serology discrepancies for the four blood types tested by both methods (Table 1). The frequency of discrepancies was widely variable between blood types and between study populations (Figure 1), reaching as high as 5.4% for N (in Southeast Asians) and 7.2% for Jkb (in Pacific Islander/Hawaiians). We additionally observed patterns in IC and LS calls which varied between blood types and between ethnicities, suggesting that underlying genetic variation may contribute to inconclusive or low signal SNP results. Conclusions: We characterized 8454 Asian and Native American blood donors for blood type by serology and with SNPs. As expected, we observed variation in the frequencies of blood type SNPs both between study populations and in comparison to Europeans. With additional testing, we found that 4.7% of donors exhibited discrepancies between SNP-predicted and serology-detected blood type, and that the frequencies of discrepancies varied between ethnic groups. We hypothesize that clinically relevant blood group gene variants were not accurately predicted using this SNP approach due to underlying genetic diversity at blood group loci in these populations. We propose that a more comprehensive approach, such as DNA sequencing, would characterize blood group gene variants in individuals of Asian and Native American heritage, as well as other genetically diverse populations. Disclosures No relevant conflicts of interest to declare.

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