Magnesium Sources and Inoculation with Azospirillum brasilense in the Initial Development of Maize

The aim was to evaluate the initial development of maize plants when submitted to mineral fertilization with magnesium sources in the presence or absence of seed inoculation with Azospirillum brasilense. To do so, was conducted an experiment in greenhouse. In the essay was adopted a randomized blocks design, in a factorial scheme 3 × 2, represented by the magnesium sources: magnesium sulfate (MgSO4), magnesium oxide (MgO) and control without Mg fertilization, in the presence or absence of inoculation with A. brasilense. The Mg sources were supplied with a dose of 30 kg ha-1, being homogenized in the substrate before sowing the crop. The analysis were carried out in the V4 stage, evaluating: basal stem diameter (BSD); relative chlorophyll content (SPAD index), leaf dry mass (LDM), stem + sheath blade dry mass (SSDM) and root dry mass (RDM). No differences were observed for the factors interaction and for the Mg sources. When considered the seed inoculation there was increases of 7.1%; 6.61%; 19.23%; 28.32%; and 15.17 %, for basal stem diameter, SPAD index, leaf dry mass, stem + sheath blade dry mass and roots, respectively. The inoculation of maize seeds with A. brasilense increases the initial development of maize plants and the SPAD index in greenhouse conditions in the V4, while the fertilization with the magnesium sources do not interfere in the maize development.

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Morphometry and production of maize inoculated with Azospirillum brasilense

Colloquium Agrariae ◽

10.5747/ca.2021.v17.n4.a447 ◽

2021 ◽

Vol 17 (4) ◽

pp. 34-46

Author(s):

Tauane Santos Brito ◽

Vandeir Francisco Guimarães ◽

Elisiane Inês Dall’Oglio Chaves ◽

Renan Pan ◽

Alexandre Wegner Lerner ◽

...

Keyword(s):

Azospirillum Brasilense ◽

Dry Mass ◽

Stem Diameter ◽

Root Volume ◽

Inoculation Methods ◽

Nutritional Parameters ◽

Seed Inoculation ◽

Maize Plants ◽

Phosphorus And Potassium ◽

Nitrogen Phosphorus

This study aimed to analyze the influence of inoculation methods with Azospirillum brasilense in morphometric and nutritional parameters of the maize crop. Maize plants, hybrid Formula VT®, were grown under different forms of inoculation: absence of inoculation; seed inoculation; leaf inoculation; seed inoculation associated to leaf inoculation. In the phenological stages V8 and VT the parameters number of leaves, aerial height, root volume, stem diameter, besides the leaves, stem, sheath, root and total dry mass and the leaf content of nitrogen, phosphorus and potassium were determined. In the R6 stage, in addition to the previous evaluations the following parameters were determined: ear diameter and length, number of kernel rows, number of kernels per row, the reproductive structure and thousand grains dry mass, and total number of grains, besides the grain’s content of nitrogen, phosphorus, and potassium. The SPAD index from the leave’s apex, medial and basal thirds was measured fortnightly. The inoculation methods with A. brasilense had little influence in maize’s morphometric and nutritional parameters, also not influencing in production. Maize plants inoculated with A. brasilense, via seed and via seed associated to leaf spraying, positively stood out for the stem diameter, leaves dry mas, root volume, and for the nitrogen, phosphorus, and potassium leaf content.

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EXCESSO DE FERRO SOBRE O DESENVOLVIMENTO DE PORTA-ENXERTOS CÍTRICOS

Nativa ◽

10.31413/nativa.v7i1.6075 ◽

2019 ◽

Vol 7 (1) ◽

pp. 50

Author(s):

Kassio Ewerton Santos Sombra ◽

Andreza Cristina Chagas de Oliveira ◽

Ronyce Do Nascimento Ferreira ◽

Alexandre Caique Costa e Silva ◽

Cleilson Do Nascimento Uchôa ◽

...

Keyword(s):

Stress Tolerance ◽

San Diego ◽

Block Design ◽

Dry Mass ◽

Stem Diameter ◽

Santa Cruz ◽

Initial Development ◽

Citrus Rootstock ◽

Randomized Complete Block Design

O trabalho objetivou avaliar os efeitos do excesso de ferro sobre a emergência e desenvolvimento inicial de genótipos de porta-enxertos cítricos. Adotou-se delineamento em blocos casualizados (DBC), em fatorial 5x2, com cinco porta-enxertos e dois substratos, repetidos quatro vezes, com parcela útil de 15 recipientes. Os genótipos consistiram do limoeiro ‘Cravo Santa Cruz’; citrandarins ‘Riverside’, ‘San Diego’ e ‘Índio’, além de, tangerineira ‘Sunki Tropical’, cultivados com ou sem óxido de ferro no substrato. Analisaram-se as porcentagens de emergência (PE) e calculou-se o índice de velocidade de emergência (IVE). Realizaram-se biometrias aos 30, 60 e 90 dias após a semeadura (DAS), mensurando-se a altura (H) e diâmetro de caule (D) das plântulas, determinando-se massa fresca (MV) e seca (MS). O excesso de ferro induziu subdesenvolvimento, e até mortalidade de plântulas já emergidas, o que refletiu diretamente nos resultados, porém, todos os genótipos apresentaram emergência superior a 75%, destacando-se o citrandarin ‘San Diego’, com maior altura e caule mais espesso, e o citrandarin ‘Riverside’ com maior sensibilidade ao estresse, registrando os menores valores. O citrandarin ’San Diego’ demonstrou tolerância o estresse e desenvolveu-se satisfatoriamente, necessitando estudos que avaliem a manutenção das características inferidas em combinação com a cultivar copa.Palavras-chave: ambiente protegido, citricultura, enxertia, estresse, mudas. EXCESS OF IRON ON THE DEVELOPMENT OF CITRUS ROOTSTOCKS ABSTRACT: The objective of this work was evaluated the effects of iron excess on the emergence and initial development of citrus rootstock genotypes. A randomized complete block design (DBC), factorial 5x2, with five rootstocks and two substrates, repeated four times, with useful plot of 15 containers was adopted. Genotypes consisted of the 'Santa Cruz' Rangpur lemon; 'Riverside', 'San Diego' and 'Indio' citrandarins, as well as 'Sunki Tropical' tangerine, grown with or without iron excess in substrate. The percentages of emergency (PE) were analyzed and the rate of emergency (IVE) was calculated. Biometry was performed at 30, 60 and 90 days after sowing (DAS), by measuring the height (H) and stem diameter (D) of the seedlings, determining fresh (MV) and dry mass (DM). The excess of iron induced underdevelopment, and even mortality of already emerged seedlings, which reflected directly in the results, however, all genotypes presented an emergency higher to 75%, standing out ’San Diego’ citrandarin, with greater height and more stem thick, and the ‘Riverside’ citrandarin with greater sensitivity to stress, registering the lowest values. 'San Diego’ citrandarin demonstrated stress tolerance and developed satisfactorily, requiring studies that evaluate maintenance of inferred characteristics in combination with cultivar canopy.Keywords: protected environment, citriculture, grafting, stress, seedlings.

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Rhizobacteria inoculation in maize associated with nitrogen and zinc fertilization at sowing

Revista Brasileira de Engenharia Agrícola e Ambiental ◽

10.1590/1807-1929/agriambi.v25n2p96-100 ◽

2021 ◽

Vol 25 (2) ◽

pp. 96-100

Author(s):

Andréia de L. Moreno ◽

Jorge F. Kusdra ◽

Angelita A. C. Picazevicz

Keyword(s):

Bacillus Subtilis ◽

Nitrogen Content ◽

Plant Height ◽

Nitrogen Fertilization ◽

Azospirillum Brasilense ◽

Dry Weight ◽

Stem Diameter ◽

Wide Range ◽

Maize Plants ◽

Total Dry Weight

ABSTRACT Rhizobacteria have a wide range of plant growth-promoting mechanisms of action, making them an alternative and/or complementary biological input for chemical fertilizers. In this respect, the present study aimed to assess growth and nitrogen accumulation in maize plants as a function of Azospirillum brasilense, Bacillus subtilis, zinc and nitrogen application at sowing. The experiment with the ‘AL Bandeirante’ maize variety was conducted in a greenhouse, using a completely randomized design. The treatments were arranged in a 2 x 2 x 2 x 2 factorial scheme, with six repetitions, considering the presence and absence of Azospirillum brasilense (5 g kg-1), Bacillus subtilis (5 mL kg-1) and zinc (20 g kg-1) in the seeds and addition or not of nitrogen (30 kg ha-1) to the soil. The variables assessed were plant height, stem diameter, leaf, stem, shoot, root and total dry weight, and shoot nitrogen content. Nitrogen fertilization in the absence of zinc increased shoot and total dry weight as well as shoot nitrogen content. Zinc applied to the seeds improved the total and stem dry weight of maize plants in the absence of Bacillus subtilis. Inoculation with Azospirillum brasilense and Bacillus subtilis increased the stem diameter and shoot nitrogen content of maize plants when nitrogen fertilization was not performed at sowing. There was no isolated or interaction effect between factors for plant height and root dry weight.

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Doses de esterco de galinha e água disponível sob o desenvolvimento inicial do milho

Revista Verde de Agroecologia e Desenvolvimento Sustentável ◽

10.18378/rvads.v11i5.4667 ◽

2016 ◽

Vol 11 (5) ◽

pp. 64

Author(s):

Francisco Marto de Souza ◽

Ellen Caroline Santos Lima ◽

Francisco Vanies da Silva Sá ◽

Lauter Silva Souto ◽

Jonathan Estivens Soares Araújo ◽

...

Keyword(s):

Dry Mass ◽

Chicken Manure ◽

Initial Growth ◽

Font Size ◽

Initial Development ◽

Available Water ◽

Chicken Litter ◽

Soil Irrigation ◽

Maize Plants ◽

Corn Plants

Objetivou-se estudar o desenvolvimento inicial de plantas de milho sob níveis de irrigação e doses de esterco de galinha. O experimento foi conduzido em condições de túnel plástico da Universidade Federal de Campina Grande, Campus de Pombal, Paraíba, no período de Dezembro de 2014 a janeiro de 2015. O arranjo dos tratamentos constituiu um fatorial 5 x 2, correspondentes a cinco doses de cama de frango e dois níveis de água disponível (AD) de 50 e 100% da CC, mantido após as irrigações do solo, com 10 tratamentos e quatro repetições, totalizando as 40 unidades experimentais. O ensaio foi realizado em unidades experimentais compostas por vasos de 18 dm3 de capacidade, semeando manualmente quatro sementes por vaso. As plantas foram conduzidas por 20 dias após a semeadura, onde foram avaliadas quanto a emergência, o crescimento inicial e o acúmulo de massa seca. As doses de 8,4 e 8,5 t ha-1 promovem o maior crescimento das plantas de milho na fase inicial de crescimento. Não houve influência da água disponível e da interação água disponível e as doses de cama de frango sobre a emergência e crescimento da plantas de milho cultivar AG 051 durante os primeiros 20 após a semeadura. Doses of chicken manure and water available under the initial development of maizeAbstract<span style="font-size: 10.0pt; font-family: 'Times New Roman','serif'; mso-bidi-font-family: 'Times New Roman'; mso-bidi-theme-font: minor-bidi; mso-ansi-language: EN-US; mso-fareast-language: PT-BR;" lang="EN-US">: The objective was to study the initial development of corn plants under irrigation levels and doses of chicken manure. The experiment was carried out in plastic tunnel conditions of the Federal University of Campina Grande, Campus of Pombal, Paraíba, from December 2014 to January 2015. The treatment arrangement consisted of a 5 x 2 factorial, corresponding to five doses of chicken litter and two levels of available water (AD) of 50 and 100% CC, maintained after soil irrigation with 10 treatments and four replications, Totaling the 40 experimental units. The experiment was carried out in experimental units composed of vessels of 18 dm3 capacity, manually seeding four seeds per pot. The plants were conducted for 20 days after sowing, where they were evaluated for emergence, initial growth and accumulation of dry mass. The doses of 8.4 and 8.5 t ha-1 promote the highest growth of maize plants in the initial phase of growth. There was no influence of available water and the interaction of available water and broiler litter doses on the emergence and growth of AG 051 maize plants during the first 20 years after sowing.Não houve influência da água disponível e da interação água disponível e as doses de cama de frango sobre a emergência e crescimento da plantas de milho cultivar AG 051 durante os primeiros 20 após a semeadura.

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Efeito da Inoculação de Azospirillum Brasilense em Plantas de Trigo Submetidas a Convivência com Azevém

Ensaios e Ciência C Biológicas Agrárias e da Saúde ◽

10.17921/1415-6938.2019v23n2p115-120 ◽

2019 ◽

Vol 23 (2) ◽

pp. 115

Author(s):

Jaqueline de Araújo Barbosa ◽

Ivonei Perego ◽

Claudia Tochetto ◽

Neumarcio Vilanova da Costa

Keyword(s):

Azospirillum Brasilense ◽

Lolium Multiflorum ◽

Block Design ◽

Dry Mass ◽

Wheat Crop ◽

Weed Competition ◽

Seed Inoculation ◽

Randomized Block Design ◽

Wheat Cultivation ◽

Number Of Leaves

A matocompetição limita o crescimento e o desenvolvimento da cultura do trigo. Porém, a inoculação com bactérias diazotróficas pode reduzir os efeitos da matocompetição. Este trabalho teve como objetivo avaliar a inoculação de Azospirillum brasilense em sementes de trigo, e seus efeitos nos estádios iniciais de desenvolvimento, sendo submetidas em convivência com plantas de azevém. Utilizou-se delineamento experimental em blocos casualizados, e esquema fatorial 2x3, com quatro repetições. O primeiro fator correspondeu a inoculação de sementes trigo com A. brasilense, e o segundo fator a três densidades populacionais de azevém (0, 25, 50). Avaliações foram realizadas nas plantas de trigo aos 18 e 34 dias após a emergência (DAE). Aos 18 DAE, na densidade de 50 plantas de azevém, as plantas de trigo inoculadas foram 30% maiores em altura. Houve redução no número de folhas, em que as plantas de trigo inoculadas apresentaram número de folhas 33% maior do que as não inoculadas, além de índice de SPAD 23% maior. Aos 34 DAE, na densidade de 50 plantas de azevém, houve aumento de 21% na altura das plantas de trigo, índice SPAD 13% maior, e área foliar 15% maior em relação às plantas não inoculadas. Apesar do aumento de 68% no acúmulo de massa seca, as plantas inoculadas não diferiram em biomassa em relação à testemunha, quando em matocompetição. Conclui-se que a inoculação com A. brasilense favorece a cultura do trigo em matocompetição até os 18 DAE, mas ao final de 34 DAE, não se observam efeitos satisfatórios. Palavras-chave: Diazotróficas. Lolium multiflorum. Matocompetição. Plantas Daninhas. AbstractThe weed competition limits the growth and development of the wheat crop. However, the inoculation with diazotrophic bacteria can reduce the effects of weed competition. The objective of this work was to evaluate the inoculation with Azospirillum brasilense in seeds, and its effects in the early stages of development, and subjected to coexistence with plants of azevém. A completely randomized block design, and factorial 2x3, with four replications were used. The first factor corresponded to wheat seed inoculation with A. brasilense, and the second factor to three population densities of azevém (0, 25, 50). Evaluations were performed in wheat plants at 18 and 34 days after emergence (DAE). At 18 DAE, the density of 50 plants of azevém, the wheat plants inoculated were 30% greater in height. There was a reduction in the number of leaves, in which the wheat plants inoculated presented a number of sheets 33% higher than those not inoculated, in addition to SPAD index 23% higher. At 34 DAE, the density of 50 plants of azevém, there was an increase of 21% in the height of wheat plants, SPAD index 13% higher leaf area, and 15% higher than the non-inoculated plants. Despite the increase of 68% in the accumulation of dry mass, the inoculated plants did not differ in biomass in relation to the control , when in weed competition. It is concluded that inoculation with A. brasilense favors wheat cultivation in weed competition up to 18 DAE, but at the end of 34 DAE, no satisfactory effects are observed. Keywords: Diazotrophic. Lolium multiflorum. Matocompetition. Weeds.

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Tratamentos Pré-Germinativos na Emergência e Desenvolvimento Inicial de Plântulas de Graviola

Ensaios e Ciência C Biológicas Agrárias e da Saúde ◽

10.17921/1415-6938.2021v25n1p72-76 ◽

2021 ◽

Vol 25 (1) ◽

pp. 72-76

Author(s):

Marcus Vinicius Sandoval Paixão ◽

Andrieli Ferrari Mônico ◽

Rafaela Barreto Cazaroto Grobério ◽

Gustavo Miranda Cremonini ◽

Hélio Pena de Faria Junior ◽

...

Keyword(s):

Seedling Emergence ◽

Dry Mass ◽

Experimental Unit ◽

Annona Muricata ◽

Initial Development ◽

Efficient Treatment ◽

Green Mass ◽

Leaf Dry Mass ◽

Number Of Leaves ◽

Collar Diameter

A graviola, Annona muricata L., produz sementes que podem apresentar dormência ocasionada pela impermeabilidade do tegumento à água, necessitando assim de métodos para superação desta. Objetivou-se avaliar o efeito de tratamentos pré-germinativos na emergência e desenvolvimento inicial de plântulas de graviola. O delineamento experimental utilizado foi em blocos ao acaso, com 13 tratamentos e quatro repetições, sendo cada unidade experimental composta de 50 sementes. As sementes foram submetidas aos tratamentos com imersão por 30 minutos, os quais foram: água (26 °C testemunha), solução de giberelina a 1.000, 2.000, 3.000 e 4.000 mg.L-1, água com gelo em ponto de fusão (0 °C), congelador por 6 horas (-10 °C), geladeira por 24 horas (10 °C), água em ponto de ebulição (100 °C), solução de NaCl 9 g.L-1, solução de KCl 5 g.L-1, água de coco e suco de laranja. A semeadura ocorreu em tubetes 280 mL contendo substrato composto por solo+eterco bovino. Trinta dias após a semeadura (DAS) avaliou-se a porcentagem, índice de velocidade e tempo médio de emergência das plântulas. Sessenta dias após a semeadura foram avaliados: número de folhas; altura da planta; diâmetro do coleto; comprimento da raiz; massa verde foliar; massa seca foliar; massa verde e massa seca da raiz. O tratamento com giberelina de 4.000 mg.L-1 de GA3 foi o tratamento mais eficiente para induzir a emergência e desenvolvimento inicial de plântulas de graviola. Palavras-chave: Annona muricata. Giberelina. Propagação. Abstract Soursop, Annona muricata L., produces seeds that may present dormancy caused by the impermeability of the integument to water, thus requiring methods to overcome it. The objective was to evaluate the effect of pre-germinative treatments on the emergence and initial development of soursop seedlings. The experimental design used was in randomized blocks, with 13 treatments and four replications, each experimental unit consisting of 50 seeds. The seeds were submitted to immersion treatments for 30 minutes, which were: water (26°C control), 1.000 gibberellin solution, 2.000, 3.000 e 4.000 mg.L-1, water with ice at melting point (0 °C), freezer for 6 hours (-10 °C), refrigerator for 24 hours (10 °C), boiling water (100°C), NaCl 9 g.L-1 solution, KCl 5 g.L-1 solution, coconut water and orange juice. Sowing took place in 280 mL tubes containing substrate composed of soil + bovine manure. Thirty days after sowing (DAS), the percentage, speed index and mean seedling emergence time were evaluated. Sixty days after sowing the following items were evaluated: number of leaves; plant height; collar diameter; root length; leaf green mass; leaf dry mass; green mass and dry mass of the root. The treatment with gibberellin of 4.000 mg.L-1 of GA3 was the most efficient treatment to induce the emergence and initial development of soursop seedlings. Keywords: Annona muricate. Gibberellin. Propagation.

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Irrigation and organic and mineral fertilization in sunflower crop

Comunicata Scientiae ◽

10.14295/cs.v8i2.1596 ◽

2018 ◽

Vol 8 (2) ◽

pp. 356-366 ◽

Cited By ~ 1

Author(s):

Krishna Ribeiro Gomes ◽

Thales Vinícius De Araújo Viana ◽

Geocleber Gomes de Sousa ◽

Fellype Rodrigo Barroso Costa ◽

Benito Moreira de Azevedo

Keyword(s):

Crop Yield ◽

Mineral Fertilizer ◽

Dry Mass ◽

Stem Diameter ◽

Mineral Fertilizers ◽

Mineral Fertilization ◽

Head Diameter ◽

Randomized Experimental Design ◽

Sunflower Crop ◽

Lower Depth

The amount of available water to certain crops can play a direct influence on the expected effect of the application of fertilizers, whether mineral or organic origin. Thus, this work was performed in order to evaluate the effects of the application of different irrigation depths and organic and mineral fertilizers in the development and production of sunflower plants in Ceará state, Brazil. The experiment was carried out in full sun in pots containing Alfisol, in a completely randomized experimental design with a 5 x 3 factorial, five irrigation depths (L1 = 50%, L2 = 75%, L3 = 100%, L4 and L5 = 125% = 150% of the Class-A Tank evaporation ECA) and three fertilizations (F1 = organic, using aerobic fermentation of Bovine biofertilizer, F2 = mineral fertilizers, NPK-based and F3 = control without an addition of fertilizers with five replications. At 65 days after sowing (DAS), the following characteristics were analyzed: number of leaves, stem diameter, and plant height. In the maximum development of achenes (about 90 DAS), the plants were harvested to determine the head diameter, dry mass of 1000 seeds, dry mass of sunflower head, seed dry mass and crop yield. The different depths applied causing a significant effect in all the sunflower crop yield parameters being as low as the effect of the applied lower depth. The application of biofertilizers in plants provided increments similar to those provided by the application of mineral fertilizers in stem diameter, dry mass of the sunflower head, the head diameter and seed dry mass under different irrigation depths. Since most applied irrigation depth (150% of the ECA), combined with the mineral fertilizer and bovine biofertilizer provided higher results in sunflower production.

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Inoculation of Maize Seeds With Azospirillum and Magnesium Through Foliar Application to Enhance Productive Performance

Journal of Agricultural Science ◽

10.5539/jas.v11n14p225 ◽

2019 ◽

Vol 11 (14) ◽

pp. 225

Author(s):

Roberto Cecatto Júnior ◽

Vandeir Francisco Guimarães ◽

Lucas Guilherme Bulegon ◽

Anderson Daniel Suss ◽

Guilherme Luiz Bazei ◽

...

Keyword(s):

Foliar Application ◽

Stem Diameter ◽

The Other ◽

Productive Performance ◽

Maize Crop ◽

Foliar Fertilization ◽

Maize Seeds ◽

Seed Inoculation ◽

Entre Rios ◽

Different Sources

The present study aimed to evaluate the productive performance of maize crop when seed inoculated with A. brasilense, associated with different foliar doses of magnesium in the crop vegetative stages. For this, two essays were conducted in field conditions, one located in Laranjeiras do Sul-PR and the other in Entre Rios do Oeste-PR. A randomized blocks scheme was used, with a 3 × 2 factorial, being the treatments with magnesium (Mg): magnesium sulphate; magnesium oxide and without magnesium, and the presence or absence of seed inoculation with A. brasilense. The magnesium sources were supplied via foliar at the V4 stage of the crop, using doses of 6 kg ha-1. Evaluations were carried at R1 determining the SPAD index and stem diameter and, at the end of the productive cycle, were evaluated production components and yield. In both sites no significant effects of foliar application with Mg were observed over the evaluated parameters. The A. brasilense inoculation provided an increase of 9.66% and 6.32% in stem diameter and of 6.8% and 6.24% in the SPAD index in Laranjeiras do Sul and Entre Rios do Oeste respectively, however, they did not increase production components and yield. It is concluded that the inoculation with A. brasilense increases of stem diameter values and SPAD index, in turn the foliar fertilization with different sources of magnesium do not interfere in the development and productivity of corn crop.

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Physical and physiological quality of corn seeds

Research Society and Development ◽

10.33448/rsd-v9i10.8687 ◽

2020 ◽

Vol 9 (10) ◽

pp. e7269108687

Author(s):

Ítala Thaísa Padilha Dubal ◽

Ivan Ricardo Carvalho ◽

João Roberto Pimentel ◽

Cristian Troyjack ◽

Vinícius Jardel Szareski ◽

...

Keyword(s):

Dry Matter ◽

Zea Mays L ◽

Shoot Length ◽

Stem Diameter ◽

Initial Growth ◽

Maize Seedlings ◽

Maize Seeds ◽

Physiological Quality ◽

Maize Plants

The aimed to evaluate the physical and physiological quality, as well the field initial performance of different maize seeds size, produced in two growing environments. It were used seeds of the Amarelo maize (Zea mays L.) genotype, where it were produced in two growing environments at Rio Grande do Sul, in the crops season of 2016/2017. The environment that the seeds are produced and the seeds form affect the maize plants initial growth. Flat seeds are tend to have a better performance on field in relation to the initial growth. The seedlings originated from Entre Ijuís-RS present superiority to shoot length, stem diameter, roots dry matter and shoot dry matter, while Pelotas-RS determines the sieves yield. The round and small seeds present lower sieves yield, shoot length, root length and stem diameter of maize seedlings.

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Selection of diazotrophic bacteria isolated from wastewater treatment plant sludge at a poultry slaughterhouse for their effect on maize plants

Revista CERES ◽

10.1590/0034-737x201865010011 ◽

2018 ◽

Vol 65 (1) ◽

pp. 85-92

Author(s):

Jorge Avelino Rodriguez Lozada ◽

Klever Cristiano Silveira ◽

Libério Junio da Silva ◽

Marihus Altoé Baldotto ◽

Lílian Estrela Borges Baldotto

Keyword(s):

Culture Media ◽

Treatment Plant ◽

Dry Weight ◽

Treated Wastewater ◽

Diazotrophic Bacteria ◽

Mineral Fertilization ◽

One Pot ◽

N Content ◽

Maize Seeds ◽

Maize Plants

ABSTRACT The economic and environmental costs of nitrogen fertilization have intensified the search for technologies that reduce mineral fertilization, for example atmospheric nitrogen-fixing (diazotrophic) bacteria inoculation. In this context, the present study addressed the isolation and quantification of diazotrophic bacteria in the sludge from treated wastewater of a poultry slaughterhouse; a description of the bacteria, based on cell and colony morphology; and an assessment of growth and N content of maize plants in response to inoculation. Sixteen morphotypes of bacteria were isolated in six N-free culture media (JMV, JMVL, NFb, JNFb, LGI, and LGI-P). The bacteria stained gram-positive, with 10 rod- and six coccoid-shaped isolates. To evaluate the potential of bacteria to promote plant growth, maize seeds were inoculated. The experiment consisted of 17 treatments (control plus 16 bacterial isolates) and was carried out in a completely randomized design with six replicates. The experimental units consisted of one pot containing two maize plants in a greenhouse. Forty-five days after planting, the variables plant height, leaf number, stem diameter, root and shoot fresh and dry weight, and N content were measured. The highest values were obtained with isolate UFV L-162, which produced 0.68 g total dry matter per plant and increased N content to 22.14 mg/plant, representing increments of 74 and 133%, respectively, compared with the control. Diazotrophs inhabit sludge from treated wastewater of poultry slaughterhouses and can potentially be used to stimulate plant development and enrich inoculants.

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