scholarly journals Pembuatan bioetanol dari rumput gajah dengan distilasi batch

2018 ◽  
Vol 8 (3) ◽  
pp. 94
Author(s):  
Ni Ketut Sari

Elephant grass is available continuously and in abundance, but has only been utilized as animal feed, and is sometimes regarded as a nuisance. However, elephant grass contains cellulose, glucose and starch that can be utilized as raw materials for ethanol production. The concentration of ethanol obtained from a study on the production of bioethanol from elephant grass was between 7-11%. To improve the purity of the ethanol, a batch distillation separation process was performed.  In the study of bioethanol production from elephant grass, a hydrolysis process was performed at the following fixed condition 30 oC temperature, 7 liter of water, 1 hour of hydrolysis time, while the following variables were changed fermentation period of 4, 5, 6, 7, and 8 days, and starter concentration of 8, 10, and 12%. From the bioethanol production study, the following best condition was obtained: 200 gram of grass, 10% Saccharomyces cerevisiae starter for 6 days. This condition produced 27.71% ethanol, with a 8.09% residual glucose. To obtain a higher purity ethanol product, a subsequent separation using batch distillation was performed, resulting in 90-95% ethanol. Therefore, elephant grass can be used as an alternative raw material for bioethanol production.Keywords: bioethanol, fermentation, hydrolysis, elephant grass Abstrak Ketersediaan rumput gajah dapat diperoleh secara kontinu dan melimpah, seringkali hanya digunakan sebagai makanan ternak, dan terkadang rumput gajah juga dianggap sebagai tanaman pengganggu. Rumput gajah mempunyai kadar selulosa, glukosa, pati yang dapat digunakan sebagai salah satu bahan penghasil etanol. Kadar etanol yang diperoleh dari kajian produksi bioetanol dari rumput gajah antara 7-11%. Untuk meningkatkan kemurnian kadar etanol dilakukan pemisahan menggunakan distilasi batch. Dalam penelitian kajian produksi bioetanol dari rumput gajah dilakukan proses hidrolisis pada kondisi tetap suhu 30 oC, air 7 liter, waktu hidrolisis 1 jam, dan kondisi berubah yaitu berat rumput gajah 50, 100, 150, 200, 250, dan 300 gram, volume larutan HCl 10, 20, 30, 40, 50 mL. Kemudian dilanjutkan proses fermentasi pada kondisi tetap suhu 30 oC, pH 4,5, volume fermentasi 500 mL dan kondisi berubah yaitu waktu fermentasi 4, 5, 6, 7, 8 hari, dan starter 8, 10, dan 12%. Dari penelitian kajian produksi bioetanol dari rumput gajah diperoleh hasil terbaik  yaitu: berat rumput gajah 200 gram, starter Saccharomyces cerevisiae 10% selama 6 hari, menghasilkan etanol sebesar 27,71% dan kadar glukosa sisa 8,09%. Untuk memperoleh produk etanol yang lebih murni dilakukan proses pemisahan lanjutan dengan distilasi batch, setelah dilakukan pemisahan lanjut diperoleh kadar etanol 90–95%, sehingga  rumput gajah dapat digunakan sebagai bahan baku alternatif pembuatan bioetanol.Kata Kunci: bioetanol, fermentasi, hidrolisis, rumput gajah.

Author(s):  
Dwi Anna Anggorowati ◽  
Sriliani Sriliani ◽  
Anis Artiyani ◽  
Harimbi Setyawati ◽  
Kevin J

Coconut husk waste is waste that has not been used optimally, generally only as a craft material. Seeing the composition of coconut husk, it has the potential to be used as an alternative fuel, one of which is to produce bioethanol products. The purpose of this research was to utilize coconut husk waste as raw material for bioethanol production and to assess the effect of the number of enzymes and time of hydrolysis on the glucose levels produced. In this research, the authors focused on obtaining glucose levels from coconut husks by hydrolysis using cellulase enzymes with an activity of 700 EGU/g. The variations used in this research were the volume of cellulase enzymes (2, 3, 4, 5, 6) ml and the hydrolysis time (4, 8, 12) hours. After the coconut husk undergoes physical and chemical treatment using 10% NaOH, there is a decrease in lignin levels from 44% to 14% and there is an increase in cellulose levels from 24% to 38%, and the use of a cellulase enzyme volume of 2 ml with a hydrolysis time of 4 hours was more optimal with a glucose level of 0.32%.


2021 ◽  
Vol 14 (2) ◽  
pp. 43
Author(s):  
Putra Oktavianto ◽  
Risdiyana Setiawan ◽  
Ilhami Ariyanti ◽  
Muhammad Fadhil Jamil

BIOETHANOL PRODUCTION FROM COCONUT HUSK USING the WET GAMMA IRRADIATION METHOD. The use of coconut husk has only been used as a material for making handicrafts such as ropes, brooms, mats, and others or just burned. The combustion of coconut husk can cause air pollution. In fact, coconut husk can be used as a raw material for bioethanol production so that the beneficial value of coconut husk will also increase. One way of bioethanol production from coconut husk is by irradiating the coconut husk. The coconut husk irradiation technique to be carried out in this study is the wet irradiation technique. Wet irradiation is carried out to accelerate the process of bioethanol production because at the time of irradiation, cellulose has been hydrolyzed and glucose has been formed so that it is more efficient in time and use of the material so that the cellulose hydrolysis process is not necessary. The coconut husk samples were wet because they were mixed with 4% NaOH and were irradiated using a gamma irradiator from STTN-BATAN Yogyakarta with a dose of 30 kGy and 50 kGy and 0 kGy (or without irradiation). Then the sample is fermented with the fungus Saccharomyces Cerevisiae from tape yeast to form ethanol. Ethanol is purified and then analyzed for concentrations using pycnometric and refractometric methods. The result is that the highest ethanol content is without irradiation (0 kGy), this is due to the low dosage used. However, the main point in this wet method research is evidence of hydrolysis of cellulose by the formation of gluoxane after irradiated wet coconut husk, and with Fehling A and B analysis, brown deposits are seen proving that glucose has been formed.


2020 ◽  
Vol 4 (2) ◽  
pp. 19
Author(s):  
Netty - Herawati

Elephant gass is cattle feed that contains good nutrition. One of its uses is converted into an energy source in the form bioethanol, Elephant grass has a high cellulose content reaching 40,85%, therefore elephant grass has the potential to be used as raw material in manufacture of bioethanol through the process of acid hydrolysis and fermentation. In research on percent yield of bioethanol from elephant grass chemically carried out at fixed conditions : grass weight 100 gr, temperature 100oC, water 1 liter, H2SO4 30 ml, hydrolysis timw 2 hours and conditions change : fermentation time 4,6,8 (day), saccharomyces cerevisiae starter 7%, 9%, 11%, 13%, HCl and H2SO4 catalys. From the research on chemical bioethanol production from elephant grass we got the best percent yield at 6 days of fermentation, 11% saccharomyces cerevisiae, HCl catalys which was 17,30%Keywords: bioethanol, fermentation, elephant grass,


Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 245
Author(s):  
Hyung-Eun An ◽  
Kang Hyun Lee ◽  
Ye Won Jang ◽  
Chang-Bae Kim ◽  
Hah Young Yoo

As greenhouse gases and environmental pollution become serious, the demand for alternative energy such as bioethanol has rapidly increased, and a large supply of biomass is required for bioenergy production. Lignocellulosic biomass is the most abundant on the planet and a large part of it, the second-generation biomass, has the advantage of not being a food resource. In this study, Sicyos angulatus, known as an invasive plant (harmful) species, was used as a raw material for bioethanol production. In order to improve enzymatic hydrolysis, S. angulatus was pretreated with different NaOH concentration at 121 °C for 10 min. The optimal NaOH concentration for the pretreatment was determined to be 2% (w/w), and the glucan content (GC) and enzymatic digestibility (ED) were 46.7% and 55.3%, respectively. Through NaOH pretreatment, the GC and ED of S. angulatus were improved by 2.4-fold and 2.5-fold, respectively, compared to the control (untreated S. angulatus). The hydrolysates from S. angulatus were applied to a medium for bioethanol fermentation of Saccharomyces cerevisiae K35. Finally, the maximum ethanol production was found to be 41.3 g based on 1000 g S. angulatus, which was 2.4-fold improved than the control group.


2000 ◽  
Vol 15 (1) ◽  
pp. 2-8 ◽  
Author(s):  
N.C. Wagner ◽  
S. Ramaswamy ◽  
U. Tschirner

AbstractA pre-economic feasibility study was undertaken to determine the potential of cereal straw for industrial utilization in Minnesota. Specifically, utilizing straw for pulp and paper manufacture was of interest. The availability of cereal straw fiber supplies at various locations across the state of Minnesota, along with pre-processing issues such as transportation, harvesting, handling, and storage, are discussed and priced. The greatest economic advantage of straw for industrial use appears to be the low cost of the raw material compared to traditional raw materials. This also provides an excellent opportunity for additional income for farmers. The methodology and information provided here should be helpful in evaluating the feasibility of utilizing straw for other industrial purposes in other parts of the world. However, in some Third World countries, long-standing on-farm, traditional uses of cereal straws for fuel, fiber, and animal feed may limit their availability for industrial utilization.


Author(s):  
Magdalena Śmiglak-Krajewska

The main purpose of this paper was to identify the factors affecting the selection of raw materials used by feed operators in feed production. An attempt was also made to indicate the barriers to increasing the use of native protein plants by feed operators in feed production. Today, many EU countries (including Poland) primarily rely on vegetable protein derived from genetically modified soya bean meal (mainly imported from South America and the U.S.) in addressing their needs for protein raw material used in animal feed. For many years now, Poland has taken steps to increase the production and use of native protein raw material to partially replace soya bean meal imports. The use of mixes of diverse domestic protein sources derived from grain legumes (peas, field beans, lupine) can provide an advantageous alternative to compound feeding stuff based on imported post-extraction soya bean meal. To meet the objective defined above, this paper relied on the results of a 2018 survey conducted with a sample of 29 feed operators located across the country. More than half (55%) of the enterprises surveyed did not use legumes in feed production; the use of legumes was above 10% in only 3% of respondents. When asked about the key factors affecting the selection of raw materials used in production processes, the respondents declared to be interested in buying large batches of homogeneous raw materials that meet specific quality parameters (33% replied “rather yes” and 67% replied “definitely yes”). The protein content of plant seeds used in feed production was identified as another aspect of extreme importance (55% replied “rather yes” and 24% replied “definitely yes”).


2018 ◽  
Vol 20 (1) ◽  
pp. 21
Author(s):  
Fitriani Kasim ◽  
Khaswar Syamsu ◽  
Dwi Setyaningsih ◽  
Prayoga Suryadarma ◽  
Sudirman Sudirman

THE EFFECT OF PRETREATMENT OF MICROWAVE HEATING ON EFFICIENCY OF HYDROLYSIS TIME AND α-CELLULOSIC CHARACTERISTICS OF PALM OIL WASTE BIOMASS. This research is underlied by the needs of various industries for alpha cellulose, especially for the needs of raw material for cellulose nanocristalline, from fiber derived from the most potential waste biomass in Indonesia, namely oil palm empty fruit bunches (OPEFB) and mesocarp fibers. This study aims to find out the effect of pretreatment by microwave heating on the efficiency of hydrolysis time and alpha cellulose characteristics from palm oil waste biomass. To produce alpha cellulose, acid hydrolysis is commonly used with the multistage pulping process method, where the fiber is dissolved into HNO3 mixed acid, 3.5% and NaNO2, heated at a temperature of 90 oC for 2 hours, hydrolyzed and delignified to remove lignin and bleached, then separated from betha and gamma cellulose by dissolving it with NaOH 17.5%. In this study, a preliminary treatment (pretreatment) with microwave heating was carried out before the fiber was hydrolyzed so that the hydrolysis process was more efficient without reducing the characteristics of the alpha cellulose produced. The research results showed that pretreatment with microwave heating can streamline the hydrolysis time from 2 hours to 1 hour, without reducing its characteristics, where the degree of fiber crystallinity (XRD) and the yield of alpha cellulose remain high. Microwave heating with 450 watts for 5 minutes (treatment A) is better than 300 watts of heating for 10 minutes (treatment B). From the results of the study, it can be concluded that the pretreatment with microwave heating can streamline the hydrolysis time of the fiber to obtain alpha cellulose and increase the yield of the produced alpha cellulose.


2018 ◽  
Vol 6 (2) ◽  
pp. 77
Author(s):  
RTM Sutamihardja ◽  
Nia Yuliani ◽  
Hana Laelasari ◽  
Devy Susanty

Acid Hydrolysis on The Starch Flour of white sweet potato (Ipomoea batatas L.) in Making of Liquid SugarNational sugar needs for both direct consumption and for industrial needs will continue to increase as the population increases. According to Dirjenbun, in 2014 the national sugar demand reaches 5.7 million tons. Consisting of 2.8 million tons of white crystalline sugar for direct community consumption and 2.9 million tons of refined crystal sugar to meet industrial needs. White sweet potato can be used as raw material for making liquid glucose through hydrolysis process with acid (HCl). The preparation of liquid glucose consists of two stages: gelatinization stage and hydrolysis stage. Optimum hydrolysis was determined by variations in HCl concentration of 0.25; 0.5; and 0.75 N and time variations of 30, 60, and 90 minutes. The yield of sweet potato starch was 28.82% and the highest yield of hydrolysis result of white sweet potato starch was 94.07% at acid concentration 0.75 N with hydrolysis time 90 minutes.Keywords: white sweet potato, Flour of white sweet potato, liquid sugar, acid hydrolysisABSTRAKKebutuhan gula nasional baik untuk konsumsi langsung maupun untuk kebutuhan industri akan terus meningkat sejalan dengan meningkatnya jumlah penduduk. Menurut Dirjenbun, pada tahun 2014 kebutuhan gula nasional mencapai 5,7 juta ton yang terdiri dari 2,8 juta ton gula kristal putih untuk konsumsi masyarakat langsung dan 2,9 juta ton gula kristal rafinasi untuk memenuhi kebutuhan industri. Ubi jalar putih dapat dijadikan bahan baku pembuatan glukosa cair melalui proses hidrolisis dengan asam (HCl). Pembuatan glukosa cair terdiri dari dua tahap yaitu tahap gelatinisasi dan tahap hidrolisis. Hidrolisis optimum ditentukan dengan variasi konsentrasi HCl yaitu 0,25; 0,5; dan 0,75 N dan variasi waktu 30, 60, dan 90 menit. Rendemen pati ubi jalar didapatkan sebesar 28,82% dan rendemen glukosa tertinggi hasil hidrolisis pati ubi jalar putih sebesar 94,07% pada konsentrasi asam 0,75 N dengan waktu hidrolisis 90 menit.Kata kunci: Ubi jalar putih, Tepung Pati Ubi Jalar Putih, Gula cair, hidrolisis asam


2016 ◽  
Vol 3 (3) ◽  
pp. 107
Author(s):  
Wagiman . ◽  
Makhmudun Ainuri ◽  
Rinda Gusvita ◽  
Jumeri .

<p>The aim of this research was study of E. cottonii to produce bioethanol fermentation substrate with a high reduction sugar content and low Hidroxymethilfurfural (HMF). Fermentation done by instant yeast and Saccharomyces cerevisiae culture of FNCC 3012.The best treatment was obtained in the combination of 2% of H2SO4 by time reaction of 120 minutes in 80°C produced 15.61 g/l reducing sugar and 5.03 g/l HMF. In fermented process, the hydrolysate with instant yeast starter delivered much more efficiency in 3.63 ml CO2 volume, 87.53% in fermentation efficiency, and 1.96 g/l reducing sugar on fifth day of fermentation. <br /><strong>Keywords</strong>: bioethanol, Eucheuma cottonii, fermentation, hydrolysis, process design</p>


2018 ◽  
Vol 41 (4) ◽  
Author(s):  
Sylvia Enid Vazquez ◽  
Luciana Buxedas ◽  
Silvana Bonifacino ◽  
Maria Belen Ramirez ◽  
Ana Lopez ◽  
...  

ABSTRACT Eucalyptus spp. residues of paper industry are a potential lignocellulosic raw material for production of second-generation bioethanol as an alternative to conventional production from cereal crops. Studying the behavior at 40 ºC of a commercial cellulase (Sunson), Eucalyptus sawdust saccharification was carried out under two pH conditions. With the aim to evaluate the bioethanol production from Eucalyptus wood, a strategy combining saccharification and Simultaneous Saccharification and Fermentation (SSF) was undertaken at 40 ºC with a thermotolerant Saccharomyces cerevisiae with different substrate and inoculum concentrations, and different nitrogen sources. At last, the process was carried out in optimal conditions with Saccharomyces cerevisiae M522 and SacSV-10. Saccharification produced more free glucose at pH 5, reaching a maximum of 1.5 g/L. Encouraging results were obtained with 500 mg/L of ammonium sulphate as a nitrogen source and 10 % v/v initial inoculum at 106 cfu/mL concentration. Yeast SacSV-10 was not inhibited by phenols present in the culture media using a wood concentration of 10 g/L, but when the solids concentration was increased, the bioprocess yield was compromised. When the process was carried out in optimal conditions the bioethanol production, expressed as the conversion percentage of cellulose to ethanol, was 71.5 % and 73.6 % for M522 and the mutant strain respectively. The studied properties of the mutant strain provide added value to it, which pose new challenges to national companies dedicated to the production and sale of inputs for bioethanol industry.


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