scholarly journals EVALUATION OF BIOETHANOL PRODUCTION FROM Eucalyptus WOOD WITH Saccharomyces cerevisiae AND SACSV-10 1

2018 ◽  
Vol 41 (4) ◽  
Author(s):  
Sylvia Enid Vazquez ◽  
Luciana Buxedas ◽  
Silvana Bonifacino ◽  
Maria Belen Ramirez ◽  
Ana Lopez ◽  
...  

ABSTRACT Eucalyptus spp. residues of paper industry are a potential lignocellulosic raw material for production of second-generation bioethanol as an alternative to conventional production from cereal crops. Studying the behavior at 40 ºC of a commercial cellulase (Sunson), Eucalyptus sawdust saccharification was carried out under two pH conditions. With the aim to evaluate the bioethanol production from Eucalyptus wood, a strategy combining saccharification and Simultaneous Saccharification and Fermentation (SSF) was undertaken at 40 ºC with a thermotolerant Saccharomyces cerevisiae with different substrate and inoculum concentrations, and different nitrogen sources. At last, the process was carried out in optimal conditions with Saccharomyces cerevisiae M522 and SacSV-10. Saccharification produced more free glucose at pH 5, reaching a maximum of 1.5 g/L. Encouraging results were obtained with 500 mg/L of ammonium sulphate as a nitrogen source and 10 % v/v initial inoculum at 106 cfu/mL concentration. Yeast SacSV-10 was not inhibited by phenols present in the culture media using a wood concentration of 10 g/L, but when the solids concentration was increased, the bioprocess yield was compromised. When the process was carried out in optimal conditions the bioethanol production, expressed as the conversion percentage of cellulose to ethanol, was 71.5 % and 73.6 % for M522 and the mutant strain respectively. The studied properties of the mutant strain provide added value to it, which pose new challenges to national companies dedicated to the production and sale of inputs for bioethanol industry.

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 245
Author(s):  
Hyung-Eun An ◽  
Kang Hyun Lee ◽  
Ye Won Jang ◽  
Chang-Bae Kim ◽  
Hah Young Yoo

As greenhouse gases and environmental pollution become serious, the demand for alternative energy such as bioethanol has rapidly increased, and a large supply of biomass is required for bioenergy production. Lignocellulosic biomass is the most abundant on the planet and a large part of it, the second-generation biomass, has the advantage of not being a food resource. In this study, Sicyos angulatus, known as an invasive plant (harmful) species, was used as a raw material for bioethanol production. In order to improve enzymatic hydrolysis, S. angulatus was pretreated with different NaOH concentration at 121 °C for 10 min. The optimal NaOH concentration for the pretreatment was determined to be 2% (w/w), and the glucan content (GC) and enzymatic digestibility (ED) were 46.7% and 55.3%, respectively. Through NaOH pretreatment, the GC and ED of S. angulatus were improved by 2.4-fold and 2.5-fold, respectively, compared to the control (untreated S. angulatus). The hydrolysates from S. angulatus were applied to a medium for bioethanol fermentation of Saccharomyces cerevisiae K35. Finally, the maximum ethanol production was found to be 41.3 g based on 1000 g S. angulatus, which was 2.4-fold improved than the control group.


2021 ◽  
Vol 14 (2) ◽  
pp. 43
Author(s):  
Putra Oktavianto ◽  
Risdiyana Setiawan ◽  
Ilhami Ariyanti ◽  
Muhammad Fadhil Jamil

BIOETHANOL PRODUCTION FROM COCONUT HUSK USING the WET GAMMA IRRADIATION METHOD. The use of coconut husk has only been used as a material for making handicrafts such as ropes, brooms, mats, and others or just burned. The combustion of coconut husk can cause air pollution. In fact, coconut husk can be used as a raw material for bioethanol production so that the beneficial value of coconut husk will also increase. One way of bioethanol production from coconut husk is by irradiating the coconut husk. The coconut husk irradiation technique to be carried out in this study is the wet irradiation technique. Wet irradiation is carried out to accelerate the process of bioethanol production because at the time of irradiation, cellulose has been hydrolyzed and glucose has been formed so that it is more efficient in time and use of the material so that the cellulose hydrolysis process is not necessary. The coconut husk samples were wet because they were mixed with 4% NaOH and were irradiated using a gamma irradiator from STTN-BATAN Yogyakarta with a dose of 30 kGy and 50 kGy and 0 kGy (or without irradiation). Then the sample is fermented with the fungus Saccharomyces Cerevisiae from tape yeast to form ethanol. Ethanol is purified and then analyzed for concentrations using pycnometric and refractometric methods. The result is that the highest ethanol content is without irradiation (0 kGy), this is due to the low dosage used. However, the main point in this wet method research is evidence of hydrolysis of cellulose by the formation of gluoxane after irradiated wet coconut husk, and with Fehling A and B analysis, brown deposits are seen proving that glucose has been formed.


2020 ◽  
Vol 9 (11) ◽  
pp. e45491110225 ◽  
Author(s):  
Anelise Christ-Ribeiro ◽  
Janaína Barreto Alves ◽  
Leonor Almeida de Souza-Soares ◽  
Eliana Badiale-Furlong

The idea that associated agro-industrial by-products only with the production of animal feed has been gradually being replaced, as the scientific community is interested in these products, as low-cost nutrients for the composition of food and/or culture media in research biotechnological to increase or improve nutrients or characteristics inherent in the raw material. Thus, this work aims to characterize input for baking with claim of natural fortification from the fermentation by Saccharomyces cerevisiae. For this, biomass was characterized in terms of its composition (ashes, fibers, lipids, proteins, and carbohydrates) and antioxidant activity of phenolic compounds. The results show an increase in nutrients such as ash, proteins and fibers, in addition the free and bound phenolic compounds present in the biomass showed antioxidant potential. Therefore, fermented rice bran is a potential input to be used in baking.


2020 ◽  
Vol 4 (2) ◽  
pp. 19
Author(s):  
Netty - Herawati

Elephant gass is cattle feed that contains good nutrition. One of its uses is converted into an energy source in the form bioethanol, Elephant grass has a high cellulose content reaching 40,85%, therefore elephant grass has the potential to be used as raw material in manufacture of bioethanol through the process of acid hydrolysis and fermentation. In research on percent yield of bioethanol from elephant grass chemically carried out at fixed conditions : grass weight 100 gr, temperature 100oC, water 1 liter, H2SO4 30 ml, hydrolysis timw 2 hours and conditions change : fermentation time 4,6,8 (day), saccharomyces cerevisiae starter 7%, 9%, 11%, 13%, HCl and H2SO4 catalys. From the research on chemical bioethanol production from elephant grass we got the best percent yield at 6 days of fermentation, 11% saccharomyces cerevisiae, HCl catalys which was 17,30%Keywords: bioethanol, fermentation, elephant grass,


2010 ◽  
pp. 111-115
Author(s):  
Péter Jobbágy

There are an enormous amount (2-3 million t/yr) of corn surplus is available year by year in Hungary. Inland utilization is an unsolved problem, whereas export facilities of raw (unprocessed) material could not be regarded as optimal way because of logistical barriers and the very low producer’s price. There are two basic opportunities for the export of the surplus of maize with reduced transportational costs and higher value: animal production and process of bio-ethanol. In Hungarian conditions both of them demand the same raw material so they should compete with each other for maize. Both need financial aid at least for the investment in order to reach profit. Decision makers are influenced by several factors in allocating of national supports between the differential branches, one of them could be the added value developing in the given vertical change. I will introduce and analyze the expectable added values of the abovementioned competitive activities.


Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 263 ◽  
Author(s):  
Celeiro ◽  
Lamas ◽  
Arcas ◽  
Lores

Three different by-products from the Eucalyptus wood industry have been exhaustively characterized to find compounds with antioxidant properties. The industrial process to manufacture Eucalyptus greenboards is distinguished by using just wood and water, which converts the generated by-products in a highly attractive source of bioactive compounds that are originally in the raw material. The studied by-products were: the screw water, derived from the washing of the wood chips; the condensates, obtained after the evaporation and further condensation of the screw water; and finally, the concentrate of eucalyptus. For all of them and for their derived organic extracts, the total polyphenols content (TPC) and antioxidant activity (AA) have been evaluated. The chromatographic fingerprints, based on gas chromatography-mass spectrometry (GC-MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS) have been obtained to identify the main extractable organic wood components. Besides, solid-phase microextraction (SPME) has been employed to characterize the most volatile compounds. Significant differences were observed for the chromatographic profiles of the studied by-products. Up to 48 and 30 different compounds were identified in the screw water, and condensate by-products, respectively; whereas the highest number of compounds, up to 72, have been identified in the organic extracts derived from the concentrate of Eucalyptus, highlighting the presence of monoterpenes, sesquiterpenes, polyphenols, and other bioactive compounds with antioxidant properties. Therefore, these by-products could be exploited to obtain natural extracts with added value which could be reused in the food, cosmetic or pharmaceutical industry, reducing the environmental impact of the industrial activity.


Holzforschung ◽  
2018 ◽  
Vol 73 (1) ◽  
pp. 3-13 ◽  
Author(s):  
Juan C. Solarte-Toro ◽  
Juan M. Romero-García ◽  
Ana Susmozas ◽  
Encarnación Ruiz ◽  
Eulogio Castro ◽  
...  

Abstract The aim of this work was to evaluate the economic feasibility of the bioethanol production (BEP) based on olive tree pruning (OTP) as a biomass feedstock with optimization of the dilute acid pretreatment in focus. For this, the BEP was simulated taking into account the influence of the operating conditions of the pretreatment stage. Then, the techno-economic results were analyzed by means of the response surface methodology (RSM). The results show that lowest price of BE was 1.94 USD l−1 with a yield of 174.12 l t−1 OTP under optimal conditions. As a conclusion, the raw material cost has a strong influence in the economic feasibility of the BEP from OTP. The inclusion of other processing lines into the process would improve the process economy.


Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 206
Author(s):  
Aleta Duque ◽  
Cristina Álvarez ◽  
Pablo Doménech ◽  
Paloma Manzanares ◽  
Antonio D. Moreno

The production of so-called advanced bioethanol offers several advantages compared to traditional bioethanol production processes in terms of sustainability criteria. This includes, for instance, the use of nonfood crops or residual biomass as raw material and a higher potential for reducing greenhouse gas emissions. The present review focuses on the recent progress related to the production of advanced bioethanol, (i) highlighting current results from using novel biomass sources such as the organic fraction of municipal solid waste and certain industrial residues (e.g., residues from the paper, food, and beverage industries); (ii) describing new developments in pretreatment technologies for the fractionation and conversion of lignocellulosic biomass, such as the bioextrusion process or the use of novel ionic liquids; (iii) listing the use of new enzyme catalysts and microbial strains during saccharification and fermentation processes. Furthermore, the most promising biorefinery approaches that will contribute to the cost-competitiveness of advanced bioethanol production processes are also discussed, focusing on innovative technologies and applications that can contribute to achieve a more sustainable and effective utilization of all biomass fractions. Special attention is given to integrated strategies such as lignocellulose-based biorefineries for the simultaneous production of bioethanol and other high added value bioproducts.


2021 ◽  
Vol 912 (1) ◽  
pp. 012008
Author(s):  
N Sembiring ◽  
H L Napitupulu ◽  
M T Sembiring ◽  
A Ishak ◽  
H A Gunawan

Abstract Eucalyptus is one of the major species used for pulp and paper industry. As industries develop, the need for Eucalyptus supply increases. However, there are challenges plantation companies must specifically determining eucalyptus market price potential risks in eucalyptus plantation and how eucalyptus plantation impacts the surrounding environment.. Stakeholders on eucalyptus supply chain have risks which have to be mitigated. The eucalyptus seedling and developer and the supplier hold crucial role in providing good quality eucalyptus raw material for pulp and paper production plants. This research is conducted to explore on ways to mitigate each stakeholders risk, challenges plantation companies face in fulfilling eucalyptus raw material for pulp and paper production plants. There are four stakeholders in eucalyptus supply chain: eucalyptus seedling developer and seller, supplier, distribution centre and pulp and paper production plants. Some of the major risk stakeholders are facing in eucalyptus supply chain are: early cut of eucalyptus trees, farmers’ lack of understanding on growing eucalyptus properly, small stem size, fluctuating eucalyptus wood price and low stock quantity. Some of the minor risk stakeholders are facing: earthquake, over fertilization, overwatering, cold climate, and plant setting. There are four methods used to determine eucalyptus pricing: cost-based pricing, customer-based pricing, competition-based pricing, and statutory pricing. Eucalyptus consume high amount of water which can cause dry areas around eucalyptus plantation area and low groundwater reservoir.


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