scholarly journals Antioxidant Effect of Saccharomyces cerevisiae Enriched with Selenium Against Pseudomonas aeruginosa Infection

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Khadijeh Mansouri-Habibabadi ◽  
Mahnoosh Fatemi ◽  
Fereshteh Ghandehari
Keyword(s):  
Oxidative Stress ◽  
Saccharomyces Cerevisiae ◽  
Pseudomonas Aeruginosa ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Blood Parameters ◽  
The Body ◽  
Control Group ◽  
Antioxidant Agents ◽  
Total Antioxidant

Background: The reaction of immune cells to the introduction of pathogens into the body is an increase in reactive oxygen species, which leads to the induction of oxidative stress. Reducing the level of oxidative stress through the use of antioxidants strengthens the immune system. Objectives: This study aimed to investigate the antioxidant effects of Saccharomyces cerevisiae and selenium-enriched S. cerevisiae against the oxidative stress induced by Pseudomonas aeruginosa in rats. Methods: A total of 36 rats were randomly divided into six groups, including control (A), S. cerevisiae (B), S. cerevisiae enriched with selenium (C), P. aeruginosa infection treated with S. cerevisiae (D), P. aeruginosa infection treated with S. cerevisiae enriched with selenium (E), and P. aeruginosa infection (F). At the end of the treatment period, the blood samples were collected to evaluate hematological parameters, total antioxidant capacity, glutathione concentration, and glutathione peroxidase activity. Results: The concentration and activity of antioxidant agents in group F were significantly decreased compared to those of the control group; however, in groups D and E, the levels of the aforementioned factors showed a significant increase compared to those of the infected group. The number of white blood cells in group F showed a significant increase, compared to that of the control group. In other groups, the observed differences in antioxidant factors and blood parameters were not significant, compared to those reported for the control group. Conclusions: The results showed that the induction of oxidative stress by P. aeruginosa was partially reduced by treatment with yeast and selenium-enriched yeast. Further studies are essential to confirm the aforementioned results.

Effect of B-ultrasound-guided nerve block on nerve block on analgesia and sedation in patients with acute cholecystitis undergoing puncture and drainage

2021 ◽  
Vol 7 (5) ◽  
pp. 3022-3028
Author(s):  
Fei Guo ◽  
Yongjun Wang ◽  
Shenghui Liu ◽  
Junrong Cao
Keyword(s):  
Oxidative Stress ◽  
Heart Rate ◽  
Acute Cholecystitis ◽  
Nerve Block ◽  
Sedation Score ◽  
The Body ◽  
Control Group ◽  
Observation Group ◽  
Ultrasound Guided ◽  
Total Antioxidant

To investigate the effect of B-ultrasound-guided nerve block on analgesia and sedation in patients with acute cholecystitis undergoing puncture and drainage. Methods 96 patients with acute cholecystitis who underwent puncture and drainage from May 2018 to November 2020 were randomly divided into control group and observation group by random number table method, with 48 cases in each group. The patients in the control group were anesthetized by local infiltration. The patients in the observation group were anesthetized by B-ultrasound-guided nerve block. Heart rate, blood pressure, analgesia score, sedation score, and oxidative stress level were compared between the two groups. Results At the time of puncture (T2), heart rate (HR) and mean arterial pressure (MAP) in the two groups were significantly lower than those before operation (T1) (P<0.05); at the end of operation (T3), HR and MAP in the control group were significantly higher than those at T1 (P<0.05); at T2, HR and MAP in the observation group were significantly higher than those in the control group (P<0.05); at T3, HR and MAP in the observation group were significantly lower than those in the control group (P<0.05). AtT2 and T3, Ramsay sedation score in both groups was significantly lower than that at T1 (P<0.05), while Ramsay score in the observation group was significantly higher than that in control group (P<0.05); atT2 and T3, visual analog scale (VAS) pain score in both groups was significantly higher than that at T1 (P<0.05), while VAS score in the observation group was significantly lower than that in the control group (P<0.05). After operation, the levels of serum malondialdehyde (MDA) and superoxide dismutase (SOD) in the two groups were significantly higher than those before operation (P<0.05), and the levels of serum total antioxidant capacity (T-AOC) were significantly lower than those before operation (P<0.05); after operation, the levels of serum SOD and T-AOC in the observation group were significantly higher than those in the control group (P<0.05), and the levels of MDA in the observation group were significantly lower than those in the control group (P>0.05). Conclusion B-ultrasound-guided nerve block anesthesia can maintain the stable hemodynamic index of patients with acute cholecystitis undergoing puncture and drainage therapy, improve the analgesic and sedative effect and reduce the oxidative stress response of the body.

scholarly journals EFFECT OF DEUTERIUM WATER ON BLOOD VALUES AND DIGESTIBILITY OF NUTRIENTS OF RHESUS MACAQUE

2020 ◽  
Vol 384 (2) ◽  
pp. 22-28 ◽  
Author(s):  
N. V. Gaponov ◽  
S. V. Svistunov ◽  
N. N. Bondarenko ◽  
I. A. Romanenko
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
The Body ◽  
Control Group ◽  
Deuterium Content ◽  
Animal Blood ◽  
Constant Weight ◽  
Effect Of Water

The use of water with a modified isotopic composition in the diet of humans and animals, for example, with a reduced deuterium content, affects the rate of biological processes. In this regard, the targeted formation of the isotopic D / H gradient in the body can be used to increase its adaptive properties to external influences. Primates have a high anatomical and physiological similarity with humans, therefore, are an indispensable model for reproducing various pathological and toxic conditions in humans. The aim of the study was to study the effect of water with a low deuterium content on the hematological and biochemical blood parameters of rhesus monkeys and on the digestibility of nutrients of the diet. In the control group, primates consumed water with a natural regional content of deuterium equal to 150 mg / l, and in the experimental group - with reduced to 50 mg/l. The duration of the experiment was 35 days. The material for the study was venous blood, serum and feces of primates. Blood was drawn from monkeys before the use of deuterium depleted water and after the completion of the experiment. All blood samples (2.5-3.0 ml) were taken from the cubital or femoral veins of animals on an empty stomach and stabilized with a heparin solution. Laboratory studies of animal blood were performed on an automatic hematological analyzer (Beckman Coulter, USA) of the CoulterAcT 5diffCP brand. In order to study the effect of water with a reduced deuterium content on hematological parameters, we determined the number of red blood cells, white blood cells, platelets, hemoglobin concentration, hematocrit, the average volume of red blood cells, red blood cell anisocytosis, and erythrocyte sedimentation rate (ESR). The metabolism trial was divided into two periods. Preliminary - to exclude the influence of previous feeding and accustoming primates to cage conditions of keeping. Experimental - conducting a thorough accounting of consumed feed, excreted excrement. Feces were collected daily at the same time (morning and evening), weighed and ground in a mortar. At each collection, 50% of the homogenized mass was taken for analysis. The collected portions were stored in the refrigerator. After the end of the experimental (accounting) period in the collected feces, the initial moisture was determined by drying at 60-70 ℃ to constant weight. The resulting air-dry mass was thoroughly ground and analyzed. It has been found that the introduction of water with a reduced deuterium content into the diet of primates has a positive effect on feed conversion rate and blood biochemical parameters.

scholarly journals Effect of Oral Contraceptive Pills on Oxidative Stress in Saudi women

2021 ◽  
Vol 7 (2) ◽  
Author(s):  
Nadia N. Osman ◽  
Dalal Mhammed Al-Mutairi
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Oral Contraceptive ◽  
Blood Cell ◽  
Hematological Parameters ◽  
Control Group ◽  
Oral Contraceptive Pills ◽  
Saudi Women ◽  
Contraceptive Pills ◽  
Total Antioxidant

Objective: The aim of this study was to evaluate the effect of oral contraceptive pills on oxidative stress in Saudi women. Methods: A total of fifty-five Saudi women were divided into two groups: users of oral contraceptive pills (OCP, N=30) for at least one year and non-users (NOCP, N=25). The demographic data were obtained through face-to-face interviews performed by the researcher. Blood specimen from both groups were drawn after 8 hours of fasting to estimate serum Total antioxidant (TAOC), Nitric oxide (NO), C-reactive protein (CRP), vitamins (E, B6, B12) and some hematological parameters: hemoglobin (Hb), Red blood cell (RBC), White blood cell (WBC). Results: The results showed a significant decreased in serum TAOC, NO, vitamins E and B6 accompanied with high significant increase in CRP level while no significant changes B12, HB, RBC and WBC were observed in the OCP users as compared to the control group. Conclusion: The results of this study indicated that the use of OCP resulted in low levels of the total antioxidant, nitric oxide, vitamin E, and B6 with a significant increase in CRP. Women who used OCP may be more susceptible to oxidative stress by enhanced depletion of antioxidants.

scholarly journals Ecotoxical influence of plumbum and cadmіum on gematological parameters of sylver carp (hypopthalmichtys molitrix)

2017 ◽  
Vol 19 (74) ◽  
pp. 24-28
Author(s):  
N. I. Hradovych
Keyword(s):  
Heavy Metals ◽  
Hematological Parameters ◽  
Silver Carp ◽  
Ecological Factors ◽  
Blood Parameters ◽  
The Body ◽  
Control Group ◽  
Research Station ◽  
Hematological Indices ◽  
Experimental Part

Many authors noticed the actuality of fish hematological parameters usage as indices of anthropogenic influence. Blood system reflects the reaction of the body on the action of different ecological factors. The problem of heavy metals influence on hematological parameters of silver cap (Hypopthalmichtys molitrix) needs to be investigated. The aim of the work was studying of ecotoxical Plumbum and Cadmium influence on hematological indices of silver carp (Hypopthalmichtys molitrix) during the period exposition. The results of the investigations concerning the influence of Plumbum and Cadmium on some hematological indices of silver carp (Hypopthalmichtys molitrix) for 12 days exposition up to the action of these heavy metals are given in this article. Experimental part was realized at the Lviv research station of Institute of fisheries NAAS (Lviv oblast, Horodok region, Velykyi Lyubin). Experimental groups contained 10 two-years individuals each. Fish were adopted to laboratory conditions for 5 days under the temperature 19 ± 2ºС, рН = 7.3 ± 0.2, oxygen content 7–8 mg/l. Blood parameters were measured on the sixth and twelfth days at the control group and groups which were under the influence on the level of 2 and 5 MPC of the appropriate metal. It was proved that the influence of Plumbum and Cadmium causes the lowering of erythrocytes number, hemoglobin and corpuscular volume: the effect becomes stronger when he concentration increases and in time. The effect from intoxication by Cadmium comes faster than by Plumbum.

scholarly journals Measurement of Pollution Level with Particulate Matter in Babylon Concrete Plant and Evaluation of Oxidative Stress and Hematological Profile of Plant Workers

2021 ◽  
pp. 3834-3841
Author(s):  
Shaimaa H. Al–Dulaimi ◽  
Adel M. Rabee
Keyword(s):  
Oxidative Stress ◽  
Particulate Matter ◽  
Glutathione Peroxidase ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Ambient Air ◽  
Pollution Level ◽  
Control Group ◽  
The Impact

The impact of exposure to different sizes of particulate matter (PM1, PM2.5, PM7, and PM10) was evaluated in  Babylon concrete plant workers who had been exposed to concrete dust for at least 10 years.  The effects of  these particles on the hematological parameters, malondialdehyde (MDA) levels, and  antioxidant enzymes (catalase and glutathione peroxidase ) were examined. The results exhibited that the levels of PM2.5 and PM10 were higher than the acceptable limits approved by the National Ambient Air Quality Standards (NAAQS). The blood parameters, namely white blood cells (WBC), red blood cell (RBC) and platelets counts, demonstrated non-significant differences between workers exposed to the PM as compared to the control group. However, differentiated white blood cells count revealed a significant increase of  polymorphonuclear leukocytes (PMN)  in exposed workers in comparison with the control group. However, both  MDA and glutathione peroxidase showed a highly significant increase in the workers ass compared to the control group. Thus, we may conclude that the concrete plant workers are exposed to a higher risk of oxidative stress that could lead to alterations in hematological parameters, enzymatic activities, and MDA level.

scholarly journals DYNAMICS OF HEMATOLOGICAL INDICES IN RATS AFTER ADMINISTRATION OF DIAMOND-CARBON COATED STEEL IMPLANTS INTO THE FEMUR

2019 ◽  
Vol 19 (3) ◽  
pp. 138-141
Author(s):  
V.B. Makarov ◽  
D.V. Morozenko ◽  
K. B. Gliebova
Keyword(s):  
Hematological Parameters ◽  
White Blood Cells ◽  
Test Group ◽  
The Body ◽  
Male Rats ◽  
Control Group ◽  
Coated Steel ◽  
Hematological Indices ◽  
Metal Implants ◽  
Body Response

Introduction. The pathological body response to metal implants is considered to be well known. There are a lot of studies devoted to this issue. This work is devoted to investigating the dynamic changes of haematological indicators as markers reflecting body response to the insertion of steel implants with diamond-carbon coating in rats. The purpose of the study is to assess the effects caused by steel implant with a diamond-coated coating on rat’s body by assessing hematological parameters. Materials and methods. The study involved 61 male rats: five of which were intact animals, and the rest was divided into two groups (28 animals in each). The first group was the control group, in which rats were inserted non-coated steel implants into the femoral metaphysic; the second group was test group, in which rats were inserted steel diamond-coated implants). The age of animals at the beginning of the experiment was 5–6 months, the body weight was 300–400 g. Blood for research was taken from the animals after decapitation in 7, 14, 30 and 90 days following the implantation. The blood samples were measured for the red blood cell, white blood cells, haemoglobin, leukogram. The findings obtained were processed by statistical analysis methods. Results and conclusions. The first group of the rats with inserted steel implants without coating showed the number of erythrocytes and haemoglobin did not differ from those in the intact rats, the leukocyte count on the 7th and 14th days was reduced by 31.8 % to 39.4 %, respectively; on the 7th day the percentage of lymphocytes and monocytes increased by 13.0 % and 5.0 % due to the toxic immunosuppressive effect of steel implants on the body of the experimental animals. The 2nd group of rats, which were inserted diamond-coated steel implants in their femurs, the rates of erythrocytopoiesis, the leukocyte number and leukogram characteristics remained unchanged during all the observation periods, except for an increase in the percentage of eosinophils on the 7th day and lymphocytes on the 90th day of follow- by 3 % that indicates a less pronounced body response in comparison with the animals of I group.

scholarly journals Hematological changes after Röntgenray exposure in radiologic technologists

2021 ◽  
Vol 25 (2) ◽  
pp. 526-531
Author(s):  
Govand Tawfeeq ◽  
Rawaz Tawfeeq ◽  
Aram Ommar ◽  
Shakar Ali
Keyword(s):  
Oxidative Stress ◽  
Teaching Hospital ◽  
Blood Cells ◽  
White Blood Cells ◽  
The Body ◽  
Radiology Department ◽  
Control Group ◽  
Convenience Sample ◽  
X Ray ◽  
Radiologic Technologists

Background and objective: In view of the known health hazards of X-ray radiation, this study focuses on the outlined effect of prolonged accidental or work-related Röntgenray exposure to hematological parameters such as red blood cells (RBCs), white blood cells (WBCs), platelets count as well as serum malondialdehyde (MDA) and glutathione in X-ray technicians. Methods: In this cross-sectional study with a comparison group, blood samples were collected over six months period from X-ray technician assistants across Rizgary teaching hospital, maternity teaching hospital, Nanakaly hospital, Erbil teaching hospital, Ashty hospital, Hundreen hospital, and Harem hospital. A convenience sample was chosen for collecting subjects exposed to X-ray at the radiology department in the hospitals mentioned above. The results were analyzed and compared with a second control group of healthy unexposed individuals. Results: It was determined that prolonged Röntgenray exposure could lead to an increase in the amount of oxidative stress as denoted by the decrease in the levels of reduced glutathione in the plasma of our test subjects. Conclusion: Although there were changes in the mean levels of plasma RBC, WBC, platelets, and malondialdehyde, the differences of these parameters between the two groups were not significant statistically. However, there was a significant reduction in glutathione levels in plasma samples of the subjects, indicating elevated oxidative stress levels within the body. Keywords: X-ray; Blood cells; Malondialdehyde; Glutathione.

Influence of DAFS-25+polizon composition on blood and functional state of liver of steers at fattening

2020 ◽  
pp. 15-18
Author(s):  
Inna R. Kilmetova ◽  
◽  
Igor A. Rodin ◽  
Nazira I. Khayrullina ◽  
Nikolay G. Fenchenko ◽  
...  
Keyword(s):  
Functional State ◽  
White Blood Cells ◽  
Live Weight ◽  
The Body ◽  
Farm Animals ◽  
Control Group ◽  
Standard Diet ◽  
The Impact ◽  
Experimental Group ◽  
Stimulation Of

Summary. The disbalanced feeding and the uneven distribution of micro- and macroelements in the environment leads to a trace element, in particular hypomelanosis. To accelerate the growth and preservation of young farm animals include in the diet of various biological additives and drugs, which include selenium. For stimulation of weight gain in the livestock industry, as well as for the prevention and treatment of pathological processes in addition to micro - and macrouse amino acids, primarily methionine. The aim of this work was to study the influence of composition of DAFS-25+Polizon on morpho-biochemical parameters of blood and functional state of the liver in fattening bulls of black-motley breed in the conditions of the Republic of Bashkortostan. Experiments using were conducted on bull-calves of black-motley breed of the properties in the properties age from 6 to 15 months. The first experimental group during the experiment was additionally given the composition of DAFS-25+Polizon at a dose of 2 mg/kg, the animals of the control group received a standard diet. To assess the impact of the composition DAFS-25+Polizon on metabolism cattle studied morphological and biochemical indicators of blood and conducted histological examination of the liver. It is established that the use of the composition of DAFS-25+Polizon at a dose of 2 mg/kg increases the number of erythrocytes and hemoglobin in the experimental group and reduces the amount of white blood cells. The serum content of total protein, phosphorus and calcium increases in the group of experimental animals. Microscopic examination of the liver revealed no changes in the structure of the organ and hepatocytes in the experimental group, whereas in the control group hemodynamic disorders and dystrophic changes in liver cells were observed. Thus, the use of the composition DAFS-25+Polizon at a dose of 2 mg/kg of live weight in fattening bulls black-and-white breed contributes to the increase of redox processes in the body, stimulation of metabolism, prevent the development of liver disorders of cellular mechanisms of metabolism, optimizes the structure of the liver, which generally provides higher productivity.

Erythrocyte and reticulocyte parameters during biological monitoring of lead exposure to the body

2020 ◽  
Vol 60 (11) ◽  
pp. 873-876
Author(s):  
Anastasiya G. Khotuleva ◽  
Mariya S. Kozyreva
Keyword(s):  
Biological Monitoring ◽  
Hematological Parameters ◽  
Lead Level ◽  
Statistical Processing ◽  
Absorption Spectrometry ◽  
The Body ◽  
Control Group ◽  
Processing Plant ◽  
Hematological Disorders ◽  
Globin Synthesis

Introduction. The most susceptible to lead is the hematopoietic system of hematopoietic organs due to lead inhibition of heme and globin synthesis and cytotoxic effect on the membrane of Mature red blood cells. The aim of study was to evaluate the informative value of the study of erythrocyte and reticulocyte parameters determined on modern hematological analyzers in patients working in contact with lead during medical and biological monitoring. Materials and methods. 45 employees of the lead battery processing plant and 30 persons of control group were examined. The level of lead in the blood was determined by atomic absorption spectrometry, δ-ALA in the urine-by the reaction of pyrol formation with acetylacetone in terms of gram of creatinine, the study of hematological parameters was performed on a Sysmex HT-2000i analyzer. Statistical processing of the results was performed using the program STATISTICA 10.0. Results. Significant changes in erythrocytic (RDW) and reticulocytic (RET, IRF, LFR, MFR, HFR, RET-He) parameters, erythropoietin in workers in contact with lead compared to the control group, changes in MCV, MCH, RDW, RET indicators in the group working in dynamics after 2 years were revealed. Associations of hematological parameters with biomarkers of exposure and effect (lead level in blood and ALA in urine) were revealed. Conclusions. Assessment of erythrocyte (MCV, MCH, RDW) and reticulocyte parameters (RET% and their distribution by maturity) in dynamics during periodic medical examinations of workers in contact with lead allows us to detect the development of hematological disorders at early stages.

Evaluation of Salivary Antioxidants and Oxidative Stress Markers in Male Smokers

2019 ◽  
Vol 22 (7) ◽  
pp. 496-501
Author(s):  
Fatemeh Ahmadi-Motamayel ◽  
Parisa Falsafi ◽  
Hamidreza Abolsamadi ◽  
Mohammad T. Goodarzi ◽  
Jalal Poorolajal
Keyword(s):  
Oxidative Stress ◽  
Uric Acid ◽  
Free Radicals ◽  
Antioxidant Capacity ◽  
Cigarette Smoke ◽  
Total Antioxidant Capacity ◽  
The Body ◽  
Total Antioxidant ◽  
The Mean ◽  
And Oxidative Stress

Background: Cigarette smoke free radicals can cause cellular damage and different diseases. All the body fluids have antioxidants which protect against free radicals. Objective: The aim of this study was to evaluate salivary total antioxidant capacity and peroxidase, uric acid and malondialdehyde levels in smokers and a nonsmoking control group. Methods: Unstimulated saliva was collected from 510 males. A total of 259 subjects were current smokers and 251 were non-smokers. The levels of salivary total antioxidant capacity, uric acid, peroxidase and malondialdehyde were measured using standard procedures. Data were analyzed with t test and ANOVA. Results: The smokers were younger and dental hygiene index was higher than healthy nonsmoking controls. The mean total antioxidant capacity in smokers and nonsmokers was 0.13±0.07 and 0.21±011, respectively (P=0.001). Smokers had significantly lower peroxidase and uric acid levels than healthy controls. In addition, the mean malondialdehyde levels in the smokers and nonsmokers were 4.55 ±2.61 and 2.79 ±2.21, respectively (P=0.001). Conclusion: Cigarette smoke produces free radical and oxidative stress, causing many side effects. Salivary antioxidant levels decreased and malondialdehyde levels increased in smokers, indicating the high oxidative stress among smokers compared to nonsmokers. Cigarette smoke had deleterious effects on main salivary antioxidants levels.

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