Effect of B-ultrasound-guided nerve block on nerve block on analgesia and sedation in patients with acute cholecystitis undergoing puncture and drainage

2021 ◽  
Vol 7 (5) ◽  
pp. 3022-3028
Author(s):  
Fei Guo ◽  
Yongjun Wang ◽  
Shenghui Liu ◽  
Junrong Cao
Keyword(s):  
Oxidative Stress ◽  
Heart Rate ◽  
Acute Cholecystitis ◽  
Nerve Block ◽  
Sedation Score ◽  
The Body ◽  
Control Group ◽  
Observation Group ◽  
Ultrasound Guided ◽  
Total Antioxidant

To investigate the effect of B-ultrasound-guided nerve block on analgesia and sedation in patients with acute cholecystitis undergoing puncture and drainage. Methods 96 patients with acute cholecystitis who underwent puncture and drainage from May 2018 to November 2020 were randomly divided into control group and observation group by random number table method, with 48 cases in each group. The patients in the control group were anesthetized by local infiltration. The patients in the observation group were anesthetized by B-ultrasound-guided nerve block. Heart rate, blood pressure, analgesia score, sedation score, and oxidative stress level were compared between the two groups. Results At the time of puncture (T2), heart rate (HR) and mean arterial pressure (MAP) in the two groups were significantly lower than those before operation (T1) (P<0.05); at the end of operation (T3), HR and MAP in the control group were significantly higher than those at T1 (P<0.05); at T2, HR and MAP in the observation group were significantly higher than those in the control group (P<0.05); at T3, HR and MAP in the observation group were significantly lower than those in the control group (P<0.05). AtT2 and T3, Ramsay sedation score in both groups was significantly lower than that at T1 (P<0.05), while Ramsay score in the observation group was significantly higher than that in control group (P<0.05); atT2 and T3, visual analog scale (VAS) pain score in both groups was significantly higher than that at T1 (P<0.05), while VAS score in the observation group was significantly lower than that in the control group (P<0.05). After operation, the levels of serum malondialdehyde (MDA) and superoxide dismutase (SOD) in the two groups were significantly higher than those before operation (P<0.05), and the levels of serum total antioxidant capacity (T-AOC) were significantly lower than those before operation (P<0.05); after operation, the levels of serum SOD and T-AOC in the observation group were significantly higher than those in the control group (P<0.05), and the levels of MDA in the observation group were significantly lower than those in the control group (P>0.05). Conclusion B-ultrasound-guided nerve block anesthesia can maintain the stable hemodynamic index of patients with acute cholecystitis undergoing puncture and drainage therapy, improve the analgesic and sedative effect and reduce the oxidative stress response of the body.

scholarly journals Antioxidant Effect of Saccharomyces cerevisiae Enriched with Selenium Against Pseudomonas aeruginosa Infection

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Khadijeh Mansouri-Habibabadi ◽  
Mahnoosh Fatemi ◽  
Fereshteh Ghandehari
Keyword(s):  
Oxidative Stress ◽  
Saccharomyces Cerevisiae ◽  
Pseudomonas Aeruginosa ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Blood Parameters ◽  
The Body ◽  
Control Group ◽  
Antioxidant Agents ◽  
Total Antioxidant

Background: The reaction of immune cells to the introduction of pathogens into the body is an increase in reactive oxygen species, which leads to the induction of oxidative stress. Reducing the level of oxidative stress through the use of antioxidants strengthens the immune system. Objectives: This study aimed to investigate the antioxidant effects of Saccharomyces cerevisiae and selenium-enriched S. cerevisiae against the oxidative stress induced by Pseudomonas aeruginosa in rats. Methods: A total of 36 rats were randomly divided into six groups, including control (A), S. cerevisiae (B), S. cerevisiae enriched with selenium (C), P. aeruginosa infection treated with S. cerevisiae (D), P. aeruginosa infection treated with S. cerevisiae enriched with selenium (E), and P. aeruginosa infection (F). At the end of the treatment period, the blood samples were collected to evaluate hematological parameters, total antioxidant capacity, glutathione concentration, and glutathione peroxidase activity. Results: The concentration and activity of antioxidant agents in group F were significantly decreased compared to those of the control group; however, in groups D and E, the levels of the aforementioned factors showed a significant increase compared to those of the infected group. The number of white blood cells in group F showed a significant increase, compared to that of the control group. In other groups, the observed differences in antioxidant factors and blood parameters were not significant, compared to those reported for the control group. Conclusions: The results showed that the induction of oxidative stress by P. aeruginosa was partially reduced by treatment with yeast and selenium-enriched yeast. Further studies are essential to confirm the aforementioned results.

Evaluation of Salivary Antioxidants and Oxidative Stress Markers in Male Smokers

2019 ◽  
Vol 22 (7) ◽  
pp. 496-501
Author(s):  
Fatemeh Ahmadi-Motamayel ◽  
Parisa Falsafi ◽  
Hamidreza Abolsamadi ◽  
Mohammad T. Goodarzi ◽  
Jalal Poorolajal
Keyword(s):  
Oxidative Stress ◽  
Uric Acid ◽  
Free Radicals ◽  
Antioxidant Capacity ◽  
Cigarette Smoke ◽  
Total Antioxidant Capacity ◽  
The Body ◽  
Total Antioxidant ◽  
The Mean ◽  
And Oxidative Stress

Background: Cigarette smoke free radicals can cause cellular damage and different diseases. All the body fluids have antioxidants which protect against free radicals. Objective: The aim of this study was to evaluate salivary total antioxidant capacity and peroxidase, uric acid and malondialdehyde levels in smokers and a nonsmoking control group. Methods: Unstimulated saliva was collected from 510 males. A total of 259 subjects were current smokers and 251 were non-smokers. The levels of salivary total antioxidant capacity, uric acid, peroxidase and malondialdehyde were measured using standard procedures. Data were analyzed with t test and ANOVA. Results: The smokers were younger and dental hygiene index was higher than healthy nonsmoking controls. The mean total antioxidant capacity in smokers and nonsmokers was 0.13±0.07 and 0.21±011, respectively (P=0.001). Smokers had significantly lower peroxidase and uric acid levels than healthy controls. In addition, the mean malondialdehyde levels in the smokers and nonsmokers were 4.55 ±2.61 and 2.79 ±2.21, respectively (P=0.001). Conclusion: Cigarette smoke produces free radical and oxidative stress, causing many side effects. Salivary antioxidant levels decreased and malondialdehyde levels increased in smokers, indicating the high oxidative stress among smokers compared to nonsmokers. Cigarette smoke had deleterious effects on main salivary antioxidants levels.

scholarly journals NF-κB and FosB mediate inflammation and oxidative stress in the blast lung injury of rats exposed to shock waves

2021 ◽  
Author(s):  
Hong Wang ◽  
Wenjuan Zhang ◽  
Jinren Liu ◽  
Junhong Gao ◽  
Le Fang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lung Injury ◽  
Shock Waves ◽  
Time Dependent ◽  
Inflammatory Factors ◽  
Control Group ◽  
Dependent Manner ◽  
Total Antioxidant ◽  
Blast Lung Injury ◽  
And Oxidative Stress

Abstract Blast lung injury (BLI) is the major cause of death in explosion-derived shock waves; however, the mechanisms of BLI are not well understood. To identify the time-dependent manner of BLI, a model of lung injury of rats induced by shock waves was established by a fuel air explosive. The model was evaluated by hematoxylin and eosin staining and pathological score. The inflammation and oxidative stress of lung injury were also investigated. The pathological scores of rats’ lung injury at 2 h, 24 h, 3 days, and 7 days post-blast were 9.75±2.96, 13.00±1.85, 8.50±1.51, and 4.00±1.41, respectively, which were significantly increased compared with those in the control group (1.13±0.64; P&lt;0.05). The respiratory frequency and pause were increased significantly, while minute expiratory volume, inspiratory time, and inspiratory peak flow rate were decreased in a time-dependent manner at 2 and 24 h post-blast compared with those in the control group. In addition, the expressions of inflammatory factors such as interleukin (IL)-6, IL-8, FosB, and NF-κB were increased significantly at 2 h and peaked at 24 h, which gradually decreased after 3 days and returned to normal in 2 weeks. The levels of total antioxidant capacity, total superoxide dismutase, and glutathione peroxidase were significantly decreased 24 h after the shock wave blast. Conversely, the malondialdehyde level reached the peak at 24 h. These results indicated that inflammatory and oxidative stress induced by shock waves changed significantly in a time-dependent manner, which may be the important factors and novel therapeutic targets for the treatment of BLI.

The Association between Oxidative Stress and Cardiac Functions in Infants Born to Preeclamptic Mothers

2018 ◽  
Vol 36 (11) ◽  
pp. 1205-1210
Author(s):  
Didem Arman ◽  
Secil Ercin ◽  
Sevilay Topcuoğlu ◽  
Ayşem Kaya ◽  
Taner Yavuz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Premature Infants ◽  
Stress Index ◽  
Control Group ◽  
Cardiac Functions ◽  
Total Antioxidant ◽  
Echocardiographic Parameters ◽  
Oxidant Status ◽  
The Relationship ◽  
And Oxidative Stress

Objective The present study aimed to assess the global oxidant and antioxidant status in infants born to preeclamptic mothers and their correlation with cardiac functions. Study Design We compared 40 infants born to preeclamptic mothers with 40 premature infants born to normotensive mothers. We assessed the relationship between echocardiographic measurements and total antioxidant capacity (TAC) and total oxidant status (TOS) values. Results In the study group, TAC, TOS, and oxidative stress index (OSI) levels were significantly higher in the cord blood (p = 0.03, 0.04, and 0.039, respectively) than in the control group. We did not observe any correlation between echocardiographic measurements and TAC, TOS, and OSI levels in infants born to preeclamptic mothers. Conclusion Compared with the control group, despite higher TAC levels in infants born to preeclamptic mothers, concurrent elevated OSI levels reveal that the oxidant–antioxidant balance is disturbed in favor of oxidants. Furthermore, the findings of this study suggest that echocardiographic parameters are unaffected by the oxidant status.

scholarly journals Perbedaan Kadar Glutation (GSH) Hepar Tikus Putih Jantan (Rattus norvegicus) yang diinduksi Parasetamol Dosis Toksik dengan Pemberian Ekstrak Daun Kelor (Moringa oleifera)

2020 ◽  
Vol 20 (1) ◽  
pp. 247
Author(s):  
Nur Insani ◽  
H.M.T Kamaluddin ◽  
Swanny Swanny
Keyword(s):  
Oxidative Stress ◽  
Treatment Group ◽  
Leaf Extract ◽  
The Body ◽  
Control Group ◽  
Bb Rat ◽  
Toxic Dose ◽  
Experimental Animals ◽  
Significant Difference ◽  
Group Ii

Glutathione (GSH) transferase deficiency due to paracetamol exposure causes further oxidative stress to liver necrosis. To reduce oxidative stress that can cause damage to the liver of the body requires antioxidants. One plant to treat liver disease is the kelor leaf (because it has an active flavonoid material also has antioxidant activity). This study was conducted to determine the difference of glutathione hepar levels of male white rat induced paracetamol toxic dose by giving kelor leaf extract. The type of research is experimental laboratory in vivo with rancagan randomized post test only control group design. With the stages as follows 1.Leaf Extract Kelor with Ethanol 96%, 2.Perpeteration of experimental animals, 3.Treatment of experimental animals by giving extract of 3-dose of kelor leaf that is KP I 250 mg / 200 gr BB rat, KP II 500 mg / 200 gr BB rat, KP III 1000 mg / 200 gr BB rat  for 14 days combined with paracetamol dose 2 gr / 200 gr BB rat compared with the negative control group (group given only paracetamol dose 2 gr / 200 gr BB rat) and control group positif only fed regular feed for 14 days). The result showed that there was a significant difference mean of GSH levels between all treatment groups obtained p = 0,000 (p <α) p values smaller than 0.05. There was the highest increase of GSH in treatment group II (142,7525 μmol / mg) and lowest dose of GSH in positive control group (57,1812 μmol / mg), dose paracetamol toxic dosage and kelor leaf extract 500 mg / gr BB rat can increase GSH hepar p = 0,000 (p <α) p less than 0 , 05. The conclusion of the test results showed that giving of kelor leaf extract at dose of treatment group II can increase GSH hepar level significantly

scholarly journals Metallothionein and Glutathione Content as Biomarkers of Metal Pollution in Mussels and Local Fishermen in Abu Qir Bay, Egypt

2016 ◽  
Vol 6 (12) ◽  
pp. 50-60 ◽  
Author(s):  
Aziza A. Saad ◽  
Amany El-Sikaily ◽  
Hany Kassem
Keyword(s):  
Oxidative Stress ◽  
Heavy Metals ◽  
Metal Pollution ◽  
The Body ◽  
Metal Exposure ◽  
Control Group ◽  
Blood Levels ◽  
Industrial Workers ◽  
High Concentrations ◽  
Abu Qir Bay

Background. When heavy metals accumulate in air, soil, and water, the risk of human exposure increases among industrial workers, as well as in people living near polluted areas. Heavy metals adversely affect a variety of bodily systems such as the cardiovascular, respiratory, endocrine, immune, and reproductive systems. In addition, long-term exposure and accumulation of heavy metals in the body may disturb oxidative stress genes and thus increase the susceptibility to various diseases. Objectives. The aim of this study is to estimate the metallothionein concentration in both mussel samples from Abu Qir Bay, Egypt and the blood of local fishermen as a biomarker of exposure to metal pollution. Methods. Levels of metallothionein and heavy metals were measured in mussels. Blood levels of metallothionein and heavy metals of local fishermen were measured and compared with a control group. The effect of heavy metal exposure on oxidative stress status was investigated through the determination of malondialdehyde (MDA), catalase and glutathione content. Results. The results of this study showed high concentrations of metallothionein in mussels and in fishermen's blood, accompanied by high concentrations of metals such as cadmium (Cd), copper (Cu), lead (Pb), chromium (Cr), and zinc (Zn). At the same time, a significant decrease in glutathione content and catalase enzyme activity was associated with a significant increase in the malondialdehyde concentrations in sera of fishermen. Conclusions. The present study found that the El Maadiya region is polluted with heavy metals, inducing oxidative stress in fishermen in the vicinity. These results reveal the necessity of further environmental monitoring in the study area in order to evaluate other types of pollutants and their effects on human health.

scholarly journals Clinical efficacy of oxidative stress correction by actovegin in diabetic polyneuropathy at type 2 diabetic patients with arterial hypertension

2010 ◽  
Vol 13 (2) ◽  
pp. 84-89
Author(s):  
Ivan Petrovich Gorshkov ◽  
Vladimir Ivanovich Zoloedov ◽  
Anna Petrovna Volynkina
Keyword(s):  
Oxidative Stress ◽  
Arterial Hypertension ◽  
Diabetic Polyneuropathy ◽  
Oxidative Capacity ◽  
Control Group ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Patients With Diabetes ◽  
Total Antioxidant

Aim. To study Actovegin efficacy in oxidative stress (OS) correction at diabetic polyneuropathy (DPN) in patients with diabetes mellitus type 2 (DM2)and arterial hypertension (AH).Materials and Methods. 51 patients (24 women and 27 men) aged 53.4?0.7 with the average duration of DM2 5.6?0.2 years, DPN - 4.9?0.2years and AH - 6.0?0.2 years were examined. Daily albuminuria, glomerular filtration rate (GRF) were evaluated, standard methods for diagnosisof DPN were used. 26 patients took Actovegin therapy during 6-8 weeks, the rest 25 patients were in the control group. Parameters of the OS werestudied. Results. The increase of total oxidative capacity, the decrease of total antioxidant capacity and the rise of levels of antibodies to oxidated LDL wererevealed in patients with DM2, DPN and AH. Antioxidant and anti-hypoxic effects of 400 mg/day of Actovegin were established in this group of patients.Conclusions. Actovegin impacts oxidative stress parameters and improves the clinical manifestation of diabetic polyneuropathy.

scholarly journals The Possible Role of Bifidobacterium longum BB536 and Lactobacillus rhamnosus HN001 on Locomotor Activity and Oxidative Stress in a Rotenone-Induced Zebrafish Model of Parkinson’s Disease

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Ovidiu-Dumitru Ilie ◽  
Emanuela Paduraru ◽  
Madalina-Andreea Robea ◽  
Ioana-Miruna Balmus ◽  
Roxana Jijie ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Locomotor Activity ◽  
Prolonged Exposure ◽  
Bifidobacterium Longum ◽  
The Body ◽  
The Other ◽  
Control Group

Background. As every organ within the body, the brain is also extremely susceptible to a plethora of noxious agents that change its chemistry. One component frequently found in current products against harmful species to crops is rotenone whose effect under prolonged exposure has been demonstrated to cause neurodegenerative disorders such as Parkinson’s disease. The latest reports have indeed revealed that rotenone promotes Parkinson’s in humans, but studies aiming to show congruent effects in zebrafish (Danio rerio) are lacking. Material and Methods. In this context, the aim of the present study was to demonstrate how chronic administration of rotenone for 3 weeks impairs the locomotor activity and sociability and induces oxidative stress in zebrafish. Results. There were no statistically significant differences following the analysis of their social interaction and locomotor tests ( p > 0.05 ). However, several exceptions have been noted in the control, rotenone, and probiotics groups when we compared their locomotor activity during the pretreatment and treatment interval ( p < 0.05 ). We further assessed the role of rotenone in disturbing the detoxifying system as represented by three enzymes known as superoxide dismutase (SOD), glutathione peroxidase (GPx), and malondialdehyde (MDA). Despite the fact that there were no statistically significant changes within SOD and GPx levels between the control group and rotenone, probiotics, and rotenone + probiotics ( p > 0.05 ), relevant changes have been observed between the analyzed groups ( p < 0.05 and p < 0.005 , respectively). On the other hand, significant differences ( p < 0.05 ) have been observed for MDA when we analyzed the data between the control group and the other three groups. Conclusions. Our results suggest that rotenone can be successfully used to trigger Parkinson’s disease-related symptomatology in zebrafish.

Effect Of Butylphthalide Combined with Ozagrel Sodium on Cerebral Perfusion and Oxidative Stress Indexes in Patients with Transient Ischemic Attack

2021 ◽  
Vol 7 (6) ◽  
pp. 6388-6394
Author(s):  
Changya Liu ◽  
Yun Li ◽  
Jianhua Zhou ◽  
Chuanxiang Wang ◽  
Tao Hu
Keyword(s):  
Oxidative Stress ◽  
Transient Ischemic Attack ◽  
Cerebral Perfusion ◽  
Reaction Rate ◽  
Effective Rate ◽  
Control Group ◽  
Observation Group ◽  
Plaque Area ◽  
Ischemic Attack ◽  
And Oxidative Stress

Background The pathogenesis of transient ischemic attack is not clear. Microthrombosis theory is a theory with high clinical recognition. Microthrombosis of carotid artery and vertebral basilar artery wall occurs shedding and vasospasm, resulting in transient cerebral insufficiency. Microembolic decomposition or distal movement, cerebrovascular dilatation, local collateral circulation after the establishment of symptoms relieved or disappeared. Most patients can be relieved in a short time, but recurrent attacks can gradually damage the structure and function of brain tissue, and increase the risk of acute cerebral infarction. The clinical treatment of this disease is mainly based on the comprehensive control of risk factors, anti thrombosis, and anti-platelet aggregation. Objective To observe the effect of butylphthalide combined with ozagrel sodium on cerebral perfusion and oxidative stress indexes in patients with transient ischemic attack. Methods A total of 116 patients diagnosed as transient ischemic attack in our hospital (February 2018 - February 2020) were selected and divided into two groups according to the treatment methods. 58 cases in the control group were treated with ozagrel sodium, 58 cases in the observation group were treated with butylphthalide combined with ozagrel sodium. The CT perfusion imaging parameters, oxidative stress indexes, plaque area, and the levels of platelet activating factor ( PAF ), α-granule membrane protein-140 ( GMP-140 ), fibrinogen ( FIB ), platelet aggregation rate (PAgT), neuron-specific enolase (NSE), oxygend-inducible factor-1α (HIF-1α), and matrix metalloproteinases 9 (MMP-9) in the two groups were recorded, and the total effective rate and adverse reaction rate were counted. Results The total effective rate of the observation group was higher than that of the control group, and the difference was statistically significant (χ2=4.640, P = 0.031). Compared with before treatment, the average time (MTT) required for the two groups of contrast agents to pass through the local brain tissue and the time (TTP) required to start the injection of contrast agents to reach the peak concentration decreased compared with before treatment. After treatment, the MTT and TTP of the observation group were shorter than those of the control group (P < 0.05). Compared with before treatment, superoxide dismutase ( SOD ) in the two groups was higher than that in the control group, PAF, GMP-140, FIB, PAgT, NSE, HIF-la, MMP-9, malondialdehyde (MDA) and plaque area decreased. After treatment, the levels of related factors in the observation group were better than those in the control group, and the plaque area was less than that in the control group (P < 0.05). There was no significant difference in the adverse reaction rate between the observation group and the control group (χ2 = 0.438, P=0.508). Conclusion Butylphthalide combined with ozagrel sodium in the treatment of transient ischemic attack can improve cerebral perfusion, reduce oxidative stress injury, reduce plaque and improve curative effect.

Lipid Peroxidation, Protein Oxidation, Gelatinases, and Their Inhibitors in a Group of Adults with Obesity

2019 ◽  
Vol 51 (06) ◽  
pp. 389-395 ◽  
Author(s):  
Gregorio Caimi ◽  
Baldassare Canino ◽  
Maria Montana ◽  
Caterina Urso ◽  
Vincenzo Calandrino ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Protein Oxidation ◽  
Thiobarbituric Acid ◽  
Protein Carbonyl ◽  
The Body ◽  
Control Group ◽  
The Matrix ◽  
Markers Of Oxidative Stress ◽  
Obese Subjects

AbstractThe association between obesity and cardiovascular diseases has a multifactorial pathogenesis, including the synthesis of inflammatory molecules, the increase in oxidative stress and the dysregulation of the matrix metalloprotease (MMP) concentration and activity. In a group of adults with obesity, divided in 2 subgroups according to the body mass index (BMI), we examined lipid peroxidation, expressed as thiobarbituric acid-reactive substances (TBARS), protein oxidation, expressed as protein carbonyl groups (PCs), plasma gelatinases (MMP-2 and MMP-9), and their tissue inhibitors (TIMP-1 and TIMP-2). In the whole group, as well as in the 2 subgroups (with BMI 30–35 or BMI>35) of obese subjects, we observed an increase in TBARS, PCs, MMP-2, and MMP-9, and also TIMP-1 and TIMP-2 in comparison with the control group. A positive correlation between TBARS and PCs emerged in obese subjects and persisted after dividing obese subjects according to BMI. The correlation between MMP-2 and TIMP-2 was not statistically significant, while a significant correlation was present between MMP-9 and TIMP-1. The correlations between the markers of oxidative stress (TBARS and PCs) and those of the MMP/TIMP profile indicated a more marked influence of protein oxidation on MMPs and TIMPs in comparison with TBARS. The innovative aspect of our study was the simultaneous evaluation of oxidative stress markers and MMP/TIMP profile in adult obese subjects. We observed significant alterations and correlations that may negatively influence the clinical course of the disease.

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