Evaluation of role of gapdh, gsk-3, b-catenin, dna fragmentation and immunohisto chemistry of caspase 3 in estimation of post mortem interval in albino rats

2020 ◽  
Vol 23 (1and2) ◽  
pp. 176
Author(s):  
Sarita Sharma ◽  
Jyoti Sarah A. Khater ◽  
Eman Abd ElHakim ◽  
Basma Emad Aboulhoda ◽  
Laila Ahmed Rashed ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Caspase 3 ◽  
Albino Rats ◽  
Post Mortem ◽  
Post Mortem Interval

scholarly journals Possible Role of Peroxynitrite in the Responses Induced by Fusicoccin in Plant Cultured Cells

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 182 ◽  
Author(s):  
Massimo Malerba ◽  
Raffaella Cerana
Keyword(s):  
Cytochrome C ◽  
Dna Fragmentation ◽  
Stress Responses ◽  
Molecular Chaperones ◽  
Caspase 3 ◽  
Cultured Cells ◽  
Acer Pseudoplatanus ◽  
Cytochrome C Release ◽  
Induced Stress

Fusicoccin (FC) is a well-known phytotoxin able to induce in Acer pseudoplatanus L. (sycamore) cultured cells, a set of responses similar to those induced by stress conditions. In this work, the possible involvement of peroxynitrite (ONOO−) in FC-induced stress responses was studied measuring both in the presence and in the absence of 2,6,8-trihydroxypurine (urate), a specific ONOO− scavenger: (1) cell death; (2) specific DNA fragmentation; (3) lipid peroxidation; (4) production of RNS and ROS; (5) activity of caspase-3-like proteases; and (6) release of cytochrome c from mitochondria, variations in the levels of molecular chaperones Hsp90 in the mitochondria and Hsp70 BiP in the endoplasmic reticulum (ER), and of regulatory 14-3-3 proteins in the cytosol. The obtained results indicate a role for ONOO− in the FC-induced responses. In particular, ONOO− seems involved in a PCD form showing apoptotic features such as specific DNA fragmentation, caspase-3-like protease activity, and cytochrome c release from mitochondria.

scholarly journals A Novel Role of SIRT1/ FGF-21 in Taurine Protection Against Cafeteria Diet-Induced Steatohepatitis in Rats

2017 ◽  
Vol 43 (2) ◽  
pp. 644-659 ◽  
Author(s):  
Azza H. Abd Elwahab ◽  
Basma K. Ramadan ◽  
Mona F. Schaalan  ◽  
Amina M. Tolba
Keyword(s):  
Caspase 3 ◽  
B Cell Lymphoma ◽  
Cafeteria Diet ◽  
Control Group ◽  
Albino Rats ◽  
Protective Mechanism ◽  
Alcoholic Fatty Liver ◽  
Group I ◽  
Tnf Α

Background: Non-alcoholic fatty liver disease (NAFLD) is one of the alarmingly rising clinical problems in the 21st century with no effective drug treatment until now. Taurine is an essential amino acid in humans that proved efficacy as a non-pharmacological therapy in a plethora of diseases; however, its impact on NAFLD remains elusive. The aim of the current study is to evaluate the protective mechanism of taurine in experimental steatohepatitis induced by junk food given as cafeteria-diet (CAF-D) in male albino rats. Methods: Forty adult male albino rats of local strain between 8-10 weeks old, weighing 150 ± 20 g, were divided into four equal groups: Group I (control group), Group II (Taurine group), Group III (CAF-D for 12 weeks) and Group IV (CAF-D +Taurine). CAF-D was given in addition to the standard chow for 12 weeks, where each rat was given one piece of beef burger fried in 15 g of sunflower oil, one teaspoonful of mayonnaise, and one piece of petit pan bread, weighing 60g/ piece. In the serum, liver function tests; ALT, AST, ALP, GGT and the lipid profile; TG, TC, HDL-C added to reduced glutathione (GSH) were assessed colorimetrically, while fibroblast growth factor (FGF)-21, adiponectin & interleukin (IL)-6 via ELISA. The same technique was used for the assays of the hepatic levels of FGF-21, silent information regulator (SIRT1), malondialdehyde (MDA),IL-10, tumor necrosis factor-α (TNF-α) as well as the apoptotic markers; caspase-3 and B-cell lymphoma (Bcl-2). Results: The cafeteria-diet induced steatohepatitis was reflected by significantly increased body and liver weight gain, elevation of liver enzymes; ALT, AST, ALP and GGT added to the dyslipidemic panel, presented as increased TC, TG, LDL-C and decreased HDL-C levels. The steatosis-induced inflammatory milieu, marked by elevated serum levels of FGF-21, IL-6, hepatic TNF-α, as well as reduced IL-10 and adiponectin, was associated with steatosis- induced hepatic oxidative stress, reflected by increased hepatic MDA and decreased GSH levels, along with stimulated caspase-3 and decline in BcL-2 hepatic levels. These pathological disturbances were significantly ameliorated by taurine supplementation and evidenced histopathologically. The cross talk between hepatic FGF-21 and SIRT1 and their association to the induced perturbations are novel findings in this study. Taurine's efficacy in restoration of hepatic structure and function is partially via the increase in SIRT1 and associated reduction of FGF-21. Conclusion: The findings of the current study prove the protective role of taurine in NAFLD via a novel role in the amelioration of FGF-21/ SIRT1 axis, which could be considered a new therapeutic target.

scholarly journals The Role of DNA Degradation in the Estimation of Post-Mortem Interval: A Systematic Review of the Current Literature

2020 ◽  
Vol 21 (10) ◽  
pp. 3540 ◽  
Author(s):  
Pamela Tozzo ◽  
Salvatore Scrivano ◽  
Matteo Sanavio ◽  
Luciana Caenazzo
Keyword(s):  
Analytical Techniques ◽  
Dna Degradation ◽  
Post Mortem ◽  
Post Mortem Interval ◽  
Target Organs ◽  
The One ◽  
Definition Of ◽  
Meta Analyses

The determination of the post-mortal interval (PMI) is an extremely discussed topic in the literature and of deep forensic interest, for which various types of methods have been proposed. The aim of the manuscript is to provide a review of the studies on the post-mortem DNA degradation used for estimating PMI. This review has been performed following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses and the PRISMA Guidelines. Several analytical techniques have been proposed to analyse the post-mortem DNA degradation in order to use it to estimate the PMI. Studies focused mainly on animal models and on particular tissues. The results have been mixed: while on the one hand literature data in this field have confirmed that in the post-mortem several degradation processes involve nucleic acids, on the other hand some fundamental aspects are still little explored: the influence of ante and post-mortem factors on DNA degradation, the feasibility and applicability of a multiparametric mathematical model that takes into account DNA degradation and the definition of one or more target organs in order to standardize the results on human cases under standard conditions.

scholarly journals Ameliorating Iron Overload in Intestinal Tissue of Adult Male Rats: Quercetin vs Deferoxamine

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Arwa A. El-Sheikh ◽  
Shimaa Hamed Ameen ◽  
Samaa Salah AbdEl-Fatah
Keyword(s):  
Oxidative Stress ◽  
Iron Overload ◽  
Adult Male ◽  
Caspase 3 ◽  
Iron Chelation ◽  
Male Rats ◽  
Albino Rats ◽  
Tissue Iron ◽  
Small Intestinal

Objective. The aim of our study is to compare the role of the new natural alternative (Quercetin) with the current iron-chelation therapy (Deferoxamine (DFO)) in the effect of iron overload on small intestinal tissues and to investigate the possible underlying molecular mechanisms of such toxicity. Methods. Forty-two adult male albino rats were divided into six groups: control groups, DFO, Quercetin, iron overload, iron overload+DFO, and iron overload+Quercetin groups. Animals received daily intraperitoneal injection of Deferoxamine (125 mg /kg), Quercetin (10 mg/kg), and ferric dextran (200 mg/kg) for 2 weeks. Results. Iron overloaded group showed significant increase in serum iron, total iron binding capacity (TIBC), transferrin saturation percentage (TS %) hepcidin (HEPC), serum ferritin, nontransferrin bound iron (NTBI), and small intestinal tissues iron levels. Iron overload significantly increased the serum oxidative stress indicator (MDA) and reduced serum total antioxidant capacity (TAC). On the other hand, iron overload increased IL6 and reduced IL10 in small intestinal tissues reflecting inflammatory condition and increased caspase 3 reactivity indicating apoptosis and increased iNOs expressing cell indicting oxidative stress especially in ileum. In addition, it induced small intestinal tissues pathological alterations. The treatment with Quercetin showed nonsignificant differences as compared to treatment with DFO that chelated the serum and tissue iron and improved the oxidative stress and reduced tissue IL6 and increased IL10 and decreased caspase 3 and iNOs expressing cells in small intestinal tissues. Moreover, it ameliorated the iron overload induced pathological alterations. Conclusion. Our study showed the potential role of Quercetin as iron chelator like DFO in case of iron overload induced small intestinal toxicity in adult rats because of its serum and tissue iron chelation, improvement of serum, and small intestinal oxidative stress, ameliorating iron induced intestinal inflammation, apoptosis, and histopathological alterations.

scholarly journals Evaluation of the Curative and Protective Role of Fresh Chicory Juice in Treatment of Hepatic Fibrosis in Male Albino Rats

2021 ◽  
Vol 14 (3) ◽  
pp. 1331-1343
Author(s):  
Ahmed Algazeery ◽  
Ahmed H. Moustafa ◽  
Ashraf S. El-Sayed ◽  
Marwa G. Rizk ◽  
Norhan A. Sabbah
Keyword(s):  
Liver Fibrosis ◽  
Lipid Profile ◽  
Dna Fragmentation ◽  
Kidney Function ◽  
Histopathological Examination ◽  
Antimicrobial Properties ◽  
Protective Role ◽  
Albino Rats ◽  
Synthetic Drugs

Background: Using synthetic drugs for treating liver fibrosis remains a challenge since, in contrast to natural products, are remarkably expensive and associated with several adverse effects. Herbs and plants showed strong antioxidant, anti-inflammatory, and antimicrobial properties. Aim: To investigate the hepatoprotective role of fresh chicory juice in delaying the immune response of hepatic cells to Carbon tetrachloride [CCl4]-induced fibrosis. Methods: Fresh chicory plant juice [50%] was given instead of drinking water to male albino rats [150-200 g]. Blood samples were collected for biochemical evaluation of liver and kidney function, antioxidant markers, lipid profile, and gene expression of TGF-ß by quantitative real-time quantification polymerase chain reaction [q PCR]. Liver tissue was removed and subjected to histopathological and genomic DNA fragmentation assay. Results: Measurements of liver enzymes, kidney function, lipid profile and levels of antioxidants confirmed the ability of chicory to protect the liver against CCl4-induced liver fibrosis by acting as a good inhibitor of TGF-ß. These results were confirmed by histopathological examination and DNA fragmentation. Conclusion: Administration of fresh chicory juice [50%] showed a significant protective role of chicory plant in delaying CCl4-induced liver fibrosis by decreasing TGF-ß.

scholarly journals Involvement of Rho/Rho kinase pathway in regulation of apoptosis in rat hepatic stellate cells

2003 ◽  
Vol 285 (5) ◽  
pp. G880-G886 ◽  
Author(s):  
Hitoshi Ikeda ◽  
Kayo Nagashima ◽  
Mikio Yanase ◽  
Tomoaki Tomiya ◽  
Masahiro Arai ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Hepatic Fibrosis ◽  
Lysophosphatidic Acid ◽  
Hepatic Stellate Cells ◽  
Therapeutic Strategy ◽  
Caspase 3 ◽  
Rho Kinase ◽  
Stellate Cells ◽  
Kinase Pathway

Hepatic stellate cells (HSCs) play a central role in the development of hepatic fibrosis. Recent evidence has revealed that HSCs also play a role in its resolution, where HSC apoptosis was determined. Moreover, induction of HSC apoptosis caused a reduction of experimental hepatic fibrosis in rats. Thus knowing the mechanism of HSC apoptosis might be important to clarify the pathophysiology and establish the therapeutic strategy for hepatic fibrosis. In HSCs, Rho and Rho kinase are known to regulate contraction, migration, and proliferation with modulation of cell morphology. Controversy exists as to the participation of Rho and Rho kinase on cell survival, and little is known regarding this matter in HSCs. In this study, we directed our focus on the role of the Rho pathway in the regulation of HSC survival. C3, an inhibitor of Rho, increased histone-associated DNA fragmentation and caspase 3 activity with enhanced condensation of nuclear chromatin in rat cultured HSCs. Moreover, Y-27632, an inhibitor of Rho kinase, had the same effects, suggesting that inhibition of the Rho/Rho kinase pathway causes HSC apoptosis. On the other hand, lysophosphatidic acid, which stimulates the Rho/Rho kinase pathway, decreased histone-associated DNA fragmentation in HSCs. Inhibition of the Rho/Rho kinase pathway did not affect p53, Bcl-2, or Bax levels in HSCs. Thus we concluded that the Rho/Rho kinase pathway may play a role in the regulation of HSC survival.

scholarly journals DNA methylation results depend on DNA integrity—role of post mortem interval

2015 ◽  
Vol 6 ◽  
Author(s):  
Mathias Rhein ◽  
Lars Hagemeier ◽  
Michael Klintschar ◽  
Marc Muschler ◽  
Stefan Bleich ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Post Mortem ◽  
Post Mortem Interval

scholarly journals The Terminalia laxiflora modulates the neurotoxicity induced by fipronil in male albino rats

2019 ◽  
Vol 39 (3) ◽  
Author(s):  
A.A. Khalaf ◽  
Mona K. Galal ◽  
Marwa A. Ibrahim ◽  
A.A. Abd Allah ◽  
Mostafa M. Afify ◽  
...  
Keyword(s):  
Caspase 3 ◽  
Methanol Extract ◽  
Reduced Glutathione ◽  
Control Group ◽  
Albino Rats ◽  
Mrna Levels ◽  
The Third ◽  
Neurotoxic Effects ◽  
Mda Content

AbstractThe extensive use of fipronil (FPN) may trigger hazards to more than insects. The present investigation was carried out to evaluate the abrogating role of Terminalia laxiflora (TL) methanol extract (TLE) against the neurotoxic effects provoked by FPN. Fourty male albino rats were assigned into four equal groups. The first group served as control, the second one was orally administered FPN (10.5 mg/kg BW), the third group was given combination of FPN and TLE) (100 mg/kg BW), and the fourth one was orally given TLE. Our findings highlighted the efficacy of TLE as a neuroprotectant through a significant reduction in malondialdehyde (MDA) content by 25.8%, elevations of the reduced glutathione (GSH) level, catalase (CAT,) and superoxide dismutase (SOD) activities by 30.9, 41.2, and 48.2% respectively. Consequently, the relative mRNA levels of both Bax and caspase-3 were down-regulated by 40.54% and caspase-3 by 30.35% compared with the control group. Moreover, restoration of the pathological tissue injuries were detected. In conclusion, TLE proved to be a potent neuroprotective agent against the FPN-induced toxicity.

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