Antioxidant properties of 5,10,15,20-tetrakis(4-hydroxypenyl)porphyrine based on electrochemical and biological researches

1970 ◽  
Author(s):  
Sergey Sergeevich Kletikov ◽  
Mariya Vasilyevna Tesakova ◽  
Vladislav Ruslanovich Kulagin ◽  
Mitar Lytovac ◽  
Vladimir Ivanovich Parfenyuk
Keyword(s):  
Antioxidant Activity ◽  
Cyclic Voltammetry ◽  
Free Radical ◽  
Body Mass ◽  
Causes Of Death ◽  
Physiological Condition ◽  
Antioxidant Properties ◽  
Living Organism ◽  
Considerable Change ◽  
Cyclic Voltammetry Method

The evaluation of antioxidant activity of porphyrin was carried out on the mutual reaction of 5,10,15,20-tetrakis(4-hydroxyphenyl)porphyrin with a free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). A cyclic voltammetry method was used to research the interaction of porphyrin and DPPH. The results of the electrochemical experiment served as a basis for testing antioxidant qualities of porphyrin on living creatures – chickens of the “Moscow-black” breed. During the duration of the research, no disturbance of physiological condition of the chickens was noticed, as well as any considerable change of body mass or causes of death. The data on blood’s antioxidant activity lets us evaluate the organism’s ability to defend itself from toxic influences of various oxygen-containing compounds. As data shows, the dilution Twin-80 with porphyrin does not lower the blood condition. During the experiment the following was noted: hydroxyl-containing porphyrin with concentrations of 10-4 mole/l lowers the amount of malondialdehyde and increases the amount of ceruloplasmin in blood, which leads to lowering the activity of oxidizing processes in a living organism, and also empowers the protein synthesizing function of the liver.

scholarly journals ESTIMATION OF ANTIOXIDANT PROPERTIES OF 5,10,15,20-TETRAKIS(4-HYDROXYPENYL)PORPHYRINE BASED ON ELECTROCHEMICAL AND BIOLOGICAL RESEARCHES

2019 ◽  
Vol 62 (3) ◽  
pp. 57-63 ◽  
Author(s):  
Sergey S. Kletikov ◽  
Mariya V. Tesakova ◽  
Vladimir I. Parfenyuk ◽  
Vladislav R. Kulagin
Keyword(s):  
Aqueous Solution ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Properties ◽  
Malonic Dialdehyde ◽  
Living Organism ◽  
Considerable Change ◽  
Control Group ◽  
Test Reaction ◽  
Fourth Group

The antioxidant activity of 5,10,15,20-tetrakis(4-hydroxyphenyl)porphyrin was established by a test reaction of interaction with free-radical 1,1-diphenyl-2-picrylhydrazyl (DPPH). A cyclic voltammetry method was used to research the interaction of porphyrin and DPPH. According to the results of the completed research, it was established that H2T(4-OHPh) P has the highest antioxidant activity among all the porphyrins compounds studied by us. The results of the electrochemical experiment served as a basis for testing antioxidant qualities of porphyrin on living creatures – chickens of the “Moscow-black” breed. Chickens were divided into 5 groups: the control group was given the basic ration, the first — the basic ration + 1% ascorbic acid solution, the second — the basic ration + an aqueous solution of Twin-80, the third — the basic ration + an aqueous solution of Twin-80 with porphyrin (10–3 mol/l), the fourth - basic ration + aqueous solution of Twin-80 with porphyrin (10-4 mol/l). In the course of the experiment, the chickens were orally administered the 2 ml solutions studied once a day in for 14 days. Wherein no disturbance of physiological condition of the chickens was noticed, as well as any considerable change of body mass or causes of death. The introduction solution Twin-80 does not impair the blood indexes. In all four experimental groups, a decrease in the concentration of malonic dialdehyde (MDA) was observed compared to the control group: the best result - a decrease in MDA by 56% is observed in the fourth group. In the three groups an increase in serum level of ceruloplasmin was observed. The greatest increase in the concentration of CP also occurs in the fourth group of chickens. With the introduction of ascorbic acid a decrease in CP relative to the control group is not observed. How it was established the 5,10,15,20-tetrakis(4-hydroxyphenyl)porphyrin with concentrations of 10-4 mole/l decline the amount of malonic dialdehyde and increases the amount of ceruloplasmin in blood, which leads to lowering the activity of oxidizing processes in a living organism and also empowers the protein synthesizing function of the liver.

scholarly journals A Facile Electrochemical Analysis to Determine Antioxidant Activity of Bee Pollen

2013 ◽  
Vol 14 ◽  
pp. 31-38 ◽  
Author(s):  
Abdelkrim Rebiai ◽  
Touhami Lanez
Keyword(s):  
Antioxidant Activity ◽  
Cyclic Voltammetry ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Electrochemical Analysis ◽  
Polyphenolic Compounds ◽  
Bee Pollen ◽  
Cyclic Voltammetry Method ◽  
High Antioxidant Activity ◽  
Phenolic And Flavonoid

The aim of this study was to determine the phenolic and flavonoid content, and antioxidant properties of tow samples of bee pollen harvested from Algeria. The content of total polyphenolic compounds, flavonoids, and antioxidant activity by the cyclic voltammetry method were determined. The contents of polyphenolic compounds was 22.68 mg/g for the pollen from Blida and 21,9 mg/g for Tipaza pollen. High antioxidant activities were found for the cyclic voltammetry method, with values that ranged from 22,3 to 27,63 mg of AAE·g–1 pollen. The bee pollen of Blida showed high antioxidant activity probably due to the high content of polyphenolic compounds present in pollen.

scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF INDIGOFERA TINCTORIA L.

2018 ◽  
Vol 11 (6) ◽  
pp. 136
Author(s):  
Chandrasekaran Swaminathan
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Root Extracts ◽  
Wide Range ◽  
Indigofera Tinctoria

Objective: The present study was designed to evaluate the phytochemical composition and antibacterial and antioxidant potential of methanolic leaf and root extracts of Indigofera tinctoria L.Methods: Phytochemical analysis was done using standard methods. The methanolic leaf and root extracts of the plant were tested against Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae, Enterobacter aerogenes, and Salmonella paratyphi B by cup-plate agar diffusion method. The free radical scavenging activities of the methanol extracts of leaves and roots were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay.Results: Phytochemical screening revealed the presence of carbohydrates, reducing sugars, alkaloids, saponins, phenolic compounds, and flavonoids in methanolic leaf and root extracts. Methanolic leaf extracts of the plant exhibited antibacterial activity against a wide range of bacteria, but the root extracts failed to inhibit the tested bacterial pathogens. The antioxidant activity determination revealed that at 100 μg/ml, methanolic root extracts had the highest antioxidant activity (89.10%) on DPPH free radicals followed by methanolic leaf extracts (46.74%).Conclusion: The results of the present study conclude that the studied plant possesses broad-spectrum antibacterial and antioxidant properties and may act as a potent antioxidant for biological systems susceptible to free radical-mediated reactions. 

scholarly journals SYNTHESIS, ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF ETHYL 2-(2-CYANO-3- (SUBSTITUTED PHENYL)ACRYLAMIDO)-4,5-DIMETHYLTHIOPHENE-3-CARBOXYLATES

2017 ◽  
Vol 10 (7) ◽  
pp. 95 ◽  
Author(s):  
Madhavi K ◽  
Sree Ramya G
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Properties ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Standard Drug ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Rat Paw

Objective: Objective of the study was to synthesize and evaluate a series of novel compounds, ethyl 2-(2-cyano-3-(substituted phenyl)acrylamido)- 4,5-dimethylthiophene-3-carboxylates, for in vitro antioxidant and in vivo anti-inflammatory activities.Methods: Ethyl 2-(2-cyano-3-(substituted phenyl)acrylamido)-4,5-dimethylthiophene-3-carboxylates were synthesized by knoevenagel condensation of active methylene group of ethyl 2-(2-cyanoacetamido)-4,5-dimethylthiophene-3-carboxylate with substituted benzaldehydes. The synthesized compounds were evaluated for their in vitro antioxidant properties in three different models, viz., reduction of 1,1-diphenyl-2-pycrylhydrazyl free radical, scavenging of nitric oxide free radical, and ferric ion-induced lipid peroxidation using rat brain homogenate. Few selected compounds with good antioxidant properties were pharmacologically evaluated for anti-inflammatory activity by carrageenan-induced rat paw edema model.Results: Clean and efficient synthetic procedure was used for the preparation of series of compounds. The structures of synthesized compounds were confirmed by infrared, 1H nuclear magnetic resonance and mass spectra. The antioxidant activity data revealed that the compounds of ethyl 2-(2-cyano-3-(substituted phenyl)acrylamido)-4,5-dimethylthiophene-3-carboxylate containing phenolic substitution showed greater antioxidant activity. Hence, the active compounds were evaluated for anti-inflammatory activity and found to possess good activity. The percentage inhibition of rat paw edema obtained for the evaluated compounds was in the range of 70.2-83.1, comparable to the standard drug diclofenac (85.0%).Conclusion: The use of inexpensive, eco-friendly and readily available reagents, easy work-up and high purity of products makes the procedure a convenient and robust method for the synthesis of title compounds. The compounds of ethyl 2-(2-cyano-3-(substituted phenyl)acrylamido)-4,5- dimethylthiophene-3-carboxylate containing phenolic substitution showed greater antioxidant and anti-inflammatory activities.

scholarly journals Antioxidant Properties of the Leaves of Chromolaena odorata Linn

2013 ◽  
Vol 35 (1) ◽  
pp. 53-57
Author(s):  
D Bhargava ◽  
CK Mondal ◽  
JN Shivapuri ◽  
S Mondal ◽  
S Kar
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Hydroxyl Radical ◽  
Methanolic Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Rational Approach ◽  
Chromolaena Odorata

 Introduction: Chromolaena odorata Linn (Asteraceae) locally known as “Banmara” has the reputation of being used as a medicinal herb in Nepal. A systemic search for drug research in now considered to be a rational approach. Therefore in the present study an effort has been made to evaluate the antioxidant activity of the leaves of the above mentioned plant. Methods: In the present research work the ethanolic and methanolic extract of leaves of the plant C. odorata Linn was assessed for free radical scavenging and antioxidant activity. In this work the ability of the extract to scavenge nitric oxide, hydroxyl radical and 1,1-diphenyl-2- picrylhydrazyl (DPPH) were used to assess its free radical scavenging potentials. Results: The DPPH radical inhibition (%) was 59.10, 52.13 and 81.12 for ethanolic extract, methanolic extract and ascorbic acid respectively. Similarly ethanolic and methanolic extract  also showed significant free radical scavenging action against nitric oxide and hydroxyl radical. Conclusion: Against the backdrop of many known medicinal properties of this plant its ethanolic and methanolic extract of leaves reveals significant antioxidant properties. DOI: http://dx.doi.org/10.2126/joim.v35i1.8900   Journal of Institute of Medicine, April, 2013; 35:53-57

scholarly journals Antioxidative activity of Silibum marianum cultivated in Mongolia

2014 ◽  
Vol 15 ◽  
pp. 53-55
Author(s):  
J Ganbaatar ◽  
O Tamir ◽  
E Lkhagvamaa ◽  
A Tsiiregzen ◽  
J Oyuntsetseg ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Antioxidative Activity ◽  
Antioxidant Properties ◽  
Milk Thistle ◽  
Dpph Assay

Silibum marianum (Milk thistle) contains high amount of phenolic compounds with antioxidant properties. The aim of this work was to evaluate the antioxidant activity of  phenolic compounds included in Milk thistle. The  antioxidant properties of the leaves and seeds of milk thistle were examined by determining its ability to scavenge the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical. The results obtained from DPPH assay confirm that Milk thistle extracts have high antioxidative activity.DOI: http://doi.dx.org/10.5564/mjc.v15i0.323 Mongolian Journal of Chemistry 15 (41), 2014, p53-55

scholarly journals COLLECTION, IDENTIFICATION, MOLECULAR CHARACTERIZATION, AND ANTIOXIDANT ACTIVITY OF NON-GILLED MUSHROOMS COLLECTED FROM NORTH WESTERN HIMALAYAS

2018 ◽  
Vol 11 (10) ◽  
pp. 254
Author(s):  
Shaveta Singh ◽  
Astha Tripathi
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Molecular Taxonomy ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Morphological Identification ◽  
Western Himalayas ◽  
Wide Range ◽  
North Western

Objective: The objective of present research deals with collection, taxonomic, molecular taxonomy characterization, and antioxidant activity of wild non-gilled mushrooms sample collected from North Western Himalayas.Methods: The wild non-gilled mushroom samples were characterized for micro, morphological properties, and molecular identification. 1,1 diphenylpicrylhydrazyl (DPPH), nitric oxide (NO2), hydrogen peroxide (H2O2), and free radical scavenging assay were used to evaluate the antioxidant properties of selected wild non-gilled mushrooms.Results: The obtained results revealed that on the basis of microscopic and morphological identification all the four non-gilled mushrooms samples were tentatively identified as 2/15 (Auricularia sp.1), 6/15 (Auricularia sp.2), 32/15 (Ganoderma sp.), and 132/15 (Trametes sp.). The colony, mycelial, and basidiospore morphology of fungal isolate were evidence of Basidiomycetes family. All these four non-gilled mushrooms were sequenced. Sequences were submitted to National Center for Biotechnology Information (NCBI), and their accession number was MF770159 (2/15), MF774107 (6/15), MF770158 (32/15), and MF770160 (132/15). The phylogenetic analysis of 18S ribosomal deoxyribonucleic acid (18S rDNA) sequence of four non-gilled mushrooms was compared with other species and identified as Auricularia polytricha, Ganoderma lucidum, Auricularia auricula-judae, and Trametes elegans. The four non-gilled mushrooms extract exhibited DPPH, NO2, and H2O2 free radical scavenging activities. G. lucidum methanolic extract has the highest effect on free radicals in comparison to hexane effect.Conclusion: All the isolated four non-gilled mushrooms showed good antioxidant potential, and it can be concluded that these mushrooms are not only consumed but also have a wide range of medicinal properties which must further explode for future use.

scholarly journals Antioxidant activity and free radicals of roasted herbal materials

2017 ◽  
Vol 63 (2) ◽  
pp. 34-41 ◽  
Author(s):  
Elżbieta Wojtowicz ◽  
Aldona Krupska ◽  
Renata Zawirska-Wojtasiak
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Raw Materials ◽  
Antioxidant Properties ◽  
Sea Buckthorn ◽  
Total Phenolic ◽  
Paramagnetic Resonance ◽  
Phenolic Contents ◽  
Roasting Process

Summary Introduction: Novel raw materials are being constantly searched for chicory coffee, which thanks to their specific composition can influence human health, thus promoting properties and of course the attractive aroma. Prior to their addition herbs – sea buckthorn (Hippophaë rhamnoides L.), rowanberry (Sorbus aucuparia L.), lovage roots (Levisticum officinale Koch) and dandelion (Taraxacum officinale coll.) – are roasted, which may change their antioxidant properties and generate free radicals with pro-oxidative properties. Objective: The characteristic of antioxidant activity of roasted herbal raw materials (sea buckthorn fruits, rowanberry, lovage roots and dandelion roots) for the production of chicory coffee and to determine the level of free radicals formed under the influence of roasting. Methods: Total phenolic contents were determined before and after the roasting process using the Folin-Ciocalteu phenol reagent and antioxidant activity by ABTS and DPPH assays. Free radicals were examined using electron paramagnetic resonance (EPR) spectroscopy. Results: The roasted materials were good sources of bioactive compounds, estimated at the total phenolic levels from 23.9 to 38.8 mg GAE/g. The highest antioxidant activity by ABTS and DPPH at 109.6 and 71.0 mg Tx/g was determined in roasted sea buckthorn. Free radicals were present in both the raw and roasted herbal materials. Roasted materials were characterised by markedly higher free radical concentrations when compared with the corresponding samples. The lowest free radical concentrations were recorded for roasted sea buckthorn fruits (0.031 × 1015 spin/g). Conclusion: Despite a high content of compounds with potent antioxidant activity, free radicals were present in novel roasted herbal materials. The formation of both antioxidant molecules and free radicals during the roasting process demonstrates that the two processes were not inversely related.

scholarly journals Impact of dietary supplements based on L-carnitine on free-radical processes in model systems

2021 ◽  
Vol 9 (4) ◽  
pp. 21-29
Author(s):  
Sh. N. Galimov ◽  
Yu. Yu. Gromenko ◽  
I. D. Gromenko ◽  
K. Sh. Galimov ◽  
I. R. Gilyazova ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Male Infertility ◽  
Dietary Supplements ◽  
Antioxidant Properties ◽  
Maximum Amplitude ◽  
Model System ◽  
Model Systems ◽  
Physiological Level ◽  
Biological Environment

Introduction. According to the results of numerous studies and meta-analyses, the effectiveness of male infertility therapy with micronutrients-antioxidants in various variants remains low, especially concerning the increase in live-birth rates and the incidence of clinical pregnancy. This may be due to both the empirical nature of such therapy with an imbalance of ejaculate redox systems, and an overdose of individual ingredients of antioxidant complexes.Purpose of the study. To evaluate the antioxidant activity of complex preparation based on L-carnitine for the treatment of male infertility in an experimental model system.Materials and methods. The dietary supplements «AndroDoz®» («Nizhfarm» JSC, Russian Federation) and «Proxeed® plus» (Sigma-Tau Pharmaceuticals Inc., Italy) were tested. The antioxidant properties of the preparations were determined in vitro by recording chemiluminescence in model systems generating reactive oxygen species using the «LKB-Wallac 1256 chemiluminometer» (Wallac Oy/PerkinElmer®, Finland). Chemiluminescence was evaluated by the light sum and the maximum amplitude of the glow, the antioxidant activity in the biological environment was studied when adding drugs to chicken yolk lipids similar to blood lipoprotein complexes.Results. The introduction of «AndroDoz®» and «Proxeed® plus» into the incubation medium in an amount comparable to the physiological level of L-carnitine in seminal plasma, inhibited the luminescence of the model system. At the same time, if «Proxeed® plus» almost completely inhibited the formation of radicals, then an equivalent dose of «AndroDoz®» reduced the intensity of chemiluminescence by 60%. Approximately in the same proportions, the preparations suppressed the intensity of lipid peroxidation processes in a model system with lipoprotein complexes similar to blood lipids, that is, the antioxidant activity was preserved in the biological environment. A decrease in the value of the chemiluminescence light sum was also found against the background of the use of these dietary supplements, which may indicate their protective effect on biological membranes, including subcellular structures of spermatozoa.Conclusion. The combined dietary supplements used in the study have a high antioxidant potential. The preparation «Proxeed® plus» can completely suppress the processes of lipoperoxidation with the possible development of a deficiency of free radicals that provide vital processes. The preparation «AndroDoz®» has a milder effect on free-radical phenomena probably due to a lower content of active ingredients and a balanced composition, which reduces the risk of complications in the form of an «antioxidant paradox» or reductive stress.

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