scholarly journals Impact of dietary supplements based on L-carnitine on free-radical processes in model systems

2021 ◽  
Vol 9 (4) ◽  
pp. 21-29
Author(s):  
Sh. N. Galimov ◽  
Yu. Yu. Gromenko ◽  
I. D. Gromenko ◽  
K. Sh. Galimov ◽  
I. R. Gilyazova ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Male Infertility ◽  
Dietary Supplements ◽  
Antioxidant Properties ◽  
Maximum Amplitude ◽  
Model System ◽  
Model Systems ◽  
Physiological Level ◽  
Biological Environment

Introduction. According to the results of numerous studies and meta-analyses, the effectiveness of male infertility therapy with micronutrients-antioxidants in various variants remains low, especially concerning the increase in live-birth rates and the incidence of clinical pregnancy. This may be due to both the empirical nature of such therapy with an imbalance of ejaculate redox systems, and an overdose of individual ingredients of antioxidant complexes.Purpose of the study. To evaluate the antioxidant activity of complex preparation based on L-carnitine for the treatment of male infertility in an experimental model system.Materials and methods. The dietary supplements «AndroDoz®» («Nizhfarm» JSC, Russian Federation) and «Proxeed® plus» (Sigma-Tau Pharmaceuticals Inc., Italy) were tested. The antioxidant properties of the preparations were determined in vitro by recording chemiluminescence in model systems generating reactive oxygen species using the «LKB-Wallac 1256 chemiluminometer» (Wallac Oy/PerkinElmer®, Finland). Chemiluminescence was evaluated by the light sum and the maximum amplitude of the glow, the antioxidant activity in the biological environment was studied when adding drugs to chicken yolk lipids similar to blood lipoprotein complexes.Results. The introduction of «AndroDoz®» and «Proxeed® plus» into the incubation medium in an amount comparable to the physiological level of L-carnitine in seminal plasma, inhibited the luminescence of the model system. At the same time, if «Proxeed® plus» almost completely inhibited the formation of radicals, then an equivalent dose of «AndroDoz®» reduced the intensity of chemiluminescence by 60%. Approximately in the same proportions, the preparations suppressed the intensity of lipid peroxidation processes in a model system with lipoprotein complexes similar to blood lipids, that is, the antioxidant activity was preserved in the biological environment. A decrease in the value of the chemiluminescence light sum was also found against the background of the use of these dietary supplements, which may indicate their protective effect on biological membranes, including subcellular structures of spermatozoa.Conclusion. The combined dietary supplements used in the study have a high antioxidant potential. The preparation «Proxeed® plus» can completely suppress the processes of lipoperoxidation with the possible development of a deficiency of free radicals that provide vital processes. The preparation «AndroDoz®» has a milder effect on free-radical phenomena probably due to a lower content of active ingredients and a balanced composition, which reduces the risk of complications in the form of an «antioxidant paradox» or reductive stress.

scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF INDIGOFERA TINCTORIA L.

2018 ◽  
Vol 11 (6) ◽  
pp. 136
Author(s):  
Chandrasekaran Swaminathan
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Root Extracts ◽  
Wide Range ◽  
Indigofera Tinctoria

Objective: The present study was designed to evaluate the phytochemical composition and antibacterial and antioxidant potential of methanolic leaf and root extracts of Indigofera tinctoria L.Methods: Phytochemical analysis was done using standard methods. The methanolic leaf and root extracts of the plant were tested against Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae, Enterobacter aerogenes, and Salmonella paratyphi B by cup-plate agar diffusion method. The free radical scavenging activities of the methanol extracts of leaves and roots were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay.Results: Phytochemical screening revealed the presence of carbohydrates, reducing sugars, alkaloids, saponins, phenolic compounds, and flavonoids in methanolic leaf and root extracts. Methanolic leaf extracts of the plant exhibited antibacterial activity against a wide range of bacteria, but the root extracts failed to inhibit the tested bacterial pathogens. The antioxidant activity determination revealed that at 100 μg/ml, methanolic root extracts had the highest antioxidant activity (89.10%) on DPPH free radicals followed by methanolic leaf extracts (46.74%).Conclusion: The results of the present study conclude that the studied plant possesses broad-spectrum antibacterial and antioxidant properties and may act as a potent antioxidant for biological systems susceptible to free radical-mediated reactions. 

Antioxidant properties of Galium verum L. (Rubiaceae) extracts

2010 ◽  
Vol 5 (3) ◽  
pp. 331-337 ◽  
Author(s):  
Neda Lakić ◽  
Neda Mimica-Dukić ◽  
Jelena Isak ◽  
Biljana Božin
Keyword(s):  
Antioxidant Activity ◽  
Corn Oil ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Model Systems ◽  
Protective Effects ◽  
Dry Extract ◽  
Scavenging Capacity ◽  
Scavenger Activity ◽  
Dried Extract

AbstractThe antioxidant properties of methanol extracts of Lady’s Bedstraw (Galium verum L., Rubiaceae) herb from two different localities in Serbia were evaluated. Antioxidant activity was assessed in four different model systems. Free radical scavenging capacity (RSC) was examined by measuring the scavenging activity of extracts on 2,2-diphenyl-1-pycrylhydrazil (DPPH) and hydroxyl radical (OH), as well as on hydrogen peroxide. In addition, the protective effects of lipid peroxidation (LP) in corn oil were evaluated by the TBA-assay using the Fe2+/ascorbate system of induction. The amount of dried extract, the content of total phenolics, flavonoids and chlorophylls was also determined. Extracts from both locations expressed very strong scavenger activity, reducing the DPPH⊙ (IC50=3.10 µg/mland 8.04 µg/ml) and OH radical formation (IC50=0.05 µg/ml and 0.54 µg/ml) and neutralising H2O2 (IC50=4.98 µg/ml and 3.80 µg/ml), in a dose dependant manner. Also, examined extracts showed notable inhibition of LP (IC50=11.69 µg/ml and 19.47 µg/ml). The observed differences in antioxidant activity could be partially explained by the levels of phenolics (2.44–4.65 mg and 4.57–5.16 mg gallic acid equivalents/g dry extract), flavonoids (6.38–10.70 µg and 15.56–17.96 µg quercetin equivalents/g dry extract) and chlorophylls in the investigated Lady’s Bedstraw extracts.

scholarly journals SYNTHESIS, ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF ETHYL 2-(2-CYANO-3- (SUBSTITUTED PHENYL)ACRYLAMIDO)-4,5-DIMETHYLTHIOPHENE-3-CARBOXYLATES

2017 ◽  
Vol 10 (7) ◽  
pp. 95 ◽  
Author(s):  
Madhavi K ◽  
Sree Ramya G
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Properties ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Standard Drug ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Rat Paw

Objective: Objective of the study was to synthesize and evaluate a series of novel compounds, ethyl 2-(2-cyano-3-(substituted phenyl)acrylamido)- 4,5-dimethylthiophene-3-carboxylates, for in vitro antioxidant and in vivo anti-inflammatory activities.Methods: Ethyl 2-(2-cyano-3-(substituted phenyl)acrylamido)-4,5-dimethylthiophene-3-carboxylates were synthesized by knoevenagel condensation of active methylene group of ethyl 2-(2-cyanoacetamido)-4,5-dimethylthiophene-3-carboxylate with substituted benzaldehydes. The synthesized compounds were evaluated for their in vitro antioxidant properties in three different models, viz., reduction of 1,1-diphenyl-2-pycrylhydrazyl free radical, scavenging of nitric oxide free radical, and ferric ion-induced lipid peroxidation using rat brain homogenate. Few selected compounds with good antioxidant properties were pharmacologically evaluated for anti-inflammatory activity by carrageenan-induced rat paw edema model.Results: Clean and efficient synthetic procedure was used for the preparation of series of compounds. The structures of synthesized compounds were confirmed by infrared, 1H nuclear magnetic resonance and mass spectra. The antioxidant activity data revealed that the compounds of ethyl 2-(2-cyano-3-(substituted phenyl)acrylamido)-4,5-dimethylthiophene-3-carboxylate containing phenolic substitution showed greater antioxidant activity. Hence, the active compounds were evaluated for anti-inflammatory activity and found to possess good activity. The percentage inhibition of rat paw edema obtained for the evaluated compounds was in the range of 70.2-83.1, comparable to the standard drug diclofenac (85.0%).Conclusion: The use of inexpensive, eco-friendly and readily available reagents, easy work-up and high purity of products makes the procedure a convenient and robust method for the synthesis of title compounds. The compounds of ethyl 2-(2-cyano-3-(substituted phenyl)acrylamido)-4,5- dimethylthiophene-3-carboxylate containing phenolic substitution showed greater antioxidant and anti-inflammatory activities.

Effect of Metal Ions (Cu2+/Fe2+) on the Antioxidant Activity of L-Ascorbic Acid-Glycine Model System

2011 ◽  
Vol 396-398 ◽  
pp. 28-31
Author(s):  
Cheng Yi Lei ◽  
Da Zhai Zhou ◽  
Rong Zhao ◽  
Qi Hui Deng ◽  
Ai Nong Yu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Maillard Reaction ◽  
Antioxidative Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Model System ◽  
Model Systems ◽  
Scavenging Activity ◽  
Metal Concentrations

The influence of Cu2+, Fe2+ on the Maillard reaction were studied by heating L-ascorbic(ASA) and glycine (Gly) solutions adjusted to pH 5 at 120±2°C for 140 min in an oil bath. The presence of metals affected the intensity of browning and intermediate products, as monitored by absorbance at 420 nm and absorbance at 294 nm, sharply increased with the increase of metal concentrations applied (0.000M, 0.005M, 0.010M, 0.015M, 0.020M). Thereafter, slight increases were observed up to 0.020M. Antioxidative activity of all MRPs derived from ASA-Gly model systems sharply increased at 0.015M (P < 0.05) and slightly changes in activity were found with increasing metal concentrations up to 0.020M. Moreover, radical-scavenging activity correlated well with browning intensity and absorbance at 294 nm.

scholarly journals Influence of microwave assisted extraction on antioxidant and antiplasmodial activities of Trichilia roka extracts

2015 ◽  
Vol 4 (5) ◽  
pp. 248-252
Author(s):  
Olga Nana ◽  
◽  
Jean Momeni ◽  
Fabrice Fekam Boyom ◽  
Martin Benoît Ngassoum ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Model Systems ◽  
Antiplasmodial Activity ◽  
Root Bark ◽  
Microwave Assisted Extraction ◽  
Mechanical Agitation ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Β Carotene

Antiplasmodial and antioxidant activities of extracts derived from Trichilia roka (Chiov) (Meliaceae) root bark were determined respectively in vitro and using two methods as well as 1,1-diphenyl-2-picrylhydrazyl radicalscavenging and β-carotene-linoleate model systems. The microwave assisted extraction extract was more effective concern antioxydant activity than the antiplasmodial activity compare to conventional mechanical agitation extraction method. Extracts obtained by microwave assisted extraction showed a high total polyphenol content of 126766 μg Equivalent of Gallic acid /g Dry Mater and a total flavonoid content of 789.22 μg Equivalent of Quercetin/g dry mater but a low antiplasmodial activity with and inhibition concentration of 48.386 μg /mL and 23.983 μg/mL for mechanical agitation extract. The evaluation of the antioxidant properties of the two extracts showed that those obtained by microwave assisted extraction shown in the 1,1-diphenyl-2-picrylhydrazyl assay and in the β-carotene bleaching test, the highest antioxidant activity respectively, with an antioxidant activity of 82.12% and with a radical-scavenging activity with inhibition percentage of 88.78%..

scholarly journals Antioxidant Properties of the Leaves of Chromolaena odorata Linn

2013 ◽  
Vol 35 (1) ◽  
pp. 53-57
Author(s):  
D Bhargava ◽  
CK Mondal ◽  
JN Shivapuri ◽  
S Mondal ◽  
S Kar
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Hydroxyl Radical ◽  
Methanolic Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Rational Approach ◽  
Chromolaena Odorata

 Introduction: Chromolaena odorata Linn (Asteraceae) locally known as “Banmara” has the reputation of being used as a medicinal herb in Nepal. A systemic search for drug research in now considered to be a rational approach. Therefore in the present study an effort has been made to evaluate the antioxidant activity of the leaves of the above mentioned plant. Methods: In the present research work the ethanolic and methanolic extract of leaves of the plant C. odorata Linn was assessed for free radical scavenging and antioxidant activity. In this work the ability of the extract to scavenge nitric oxide, hydroxyl radical and 1,1-diphenyl-2- picrylhydrazyl (DPPH) were used to assess its free radical scavenging potentials. Results: The DPPH radical inhibition (%) was 59.10, 52.13 and 81.12 for ethanolic extract, methanolic extract and ascorbic acid respectively. Similarly ethanolic and methanolic extract  also showed significant free radical scavenging action against nitric oxide and hydroxyl radical. Conclusion: Against the backdrop of many known medicinal properties of this plant its ethanolic and methanolic extract of leaves reveals significant antioxidant properties. DOI: http://dx.doi.org/10.2126/joim.v35i1.8900   Journal of Institute of Medicine, April, 2013; 35:53-57

Antioxidant Activity of Some Selected East Borneo Plants

2015 ◽  
Vol 4 (1) ◽  
pp. 58
Author(s):  
Islamudin Ahmad ◽  
Risky Sulistiarini ◽  
Laode Rijai
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Native Plants ◽  
Ethyl Acetate Extract ◽  
Model Systems ◽  
Brucea Javanica ◽  
Butanol Extract ◽  
Sonneratia Caseolaris ◽  
Free Radical Activity

The native plants of East Borneo the <em>Costus specious </em>(Koening) J.E. Smith stem, <em>Lagerstroema spesiosa</em> Pers leaf, <em>Cerbera mangans </em>L leaf, <em>Vitis trifolia </em>L fruit., <em>Scurrula atropurpurea </em>(Blume) Danser root, <em>Brucea javanica </em>(L.) Merr. leaf, <em>Lygodium microphyllum, Bidens Chinensis </em>Willd., <em>Sonneratia caseolaris </em>L. peel, <em>Sonneratia caseolaris </em>L. stem is almost under-explored for their potensial benefits. They were extracted by the solvents of increasing polarity (n-hexane, ethyl acetate, and n-butanol) were tested for their free radical activity against DPPH (2,2-diphenyl-1-picrylhydrazyl). The ethyl acetate extract of <em>Costus spesiosus </em>(Koening) J. E. Smith antioxidant were screnned concentration of 68 ppm, similarly athyl acetate extract of <em>Vitis trivolia</em> L., showed antioxidant activity at 64,30 ppm. As the ethyl acetate extract of <em>Scurrulla atropurpurea </em>(Blume), <em>Brucea javanica</em> (L.) Merr, <em>Lygodium microphyllum</em> and <em>Sonneratia caseolaris</em> L. stem, showed antioxidant activity at 273,52 ppm, 91,12 ppm, 17,39 ppm and 7,03 ppm. N-butanol extract of <em>Lagerstroema spesiosa </em>Pers,<em> Cerbera mangans</em> L, <em>Bidens chinensis</em> Willd, and <em>Sonneratia caseolaris</em> L. peel showed 8,37 ppm, 128,59 ppm, 18,17 ppm and 54,29 ppm antioxidant activity using DPPH model systems. Owing to the property, the studies can be further extended to exploit them for their possible application for preservation of food products as well as their use as health supplements.

scholarly journals Assessment of the Antioxidant Ability of Thymus albicans, T. mastichina, T. camphoratus and T. carnosus Essential Oils by TBARS and Micellar Model systems

2007 ◽  
Vol 2 (4) ◽  
pp. 1934578X0700200 ◽  
Author(s):  
M. Graça Miguel ◽  
Ludmila A. Costa ◽  
A. Cristina Figueiredo ◽  
José G. Barroso ◽  
Luís G. Pedro
Keyword(s):  
Antioxidant Activity ◽  
Essential Oils ◽  
Model System ◽  
Model Systems ◽  
Antioxidant Ability ◽  
Vegetative Phase ◽  
The Third ◽  
Different Populations ◽  
Main Components ◽  
Tbars Assay

The essential oils isolated by hydrodistillation from different populations of Thymus albicans, T. mastichina, T. carnosus and T. camphoratus, collected during the vegetative phase, were analysed by GC and GC-MS. The same oils were used in the antioxidant activity evaluation using the TBARS assay and the micellar model systems. T. albicans oils and two of the oils from T. mastichina populations were 1,8-cineole-rich. The third T. mastichina oil was linalool-rich. trans-Sabinene hydrate, borneol and linalool in similar amounts, and 1,8-cineole were the main components of the oils from each of the three populations of T. camphoratus. T. carnosus oils were mainly composed of borneol. All essential oils revealed some degree of antioxidant activity either in the presence or absence of ABAP in the TBARS method. The micellar model system showed higher antioxidant indices than those obtained by the TBARS method.

scholarly journals Antioxidative activity of Silibum marianum cultivated in Mongolia

2014 ◽  
Vol 15 ◽  
pp. 53-55
Author(s):  
J Ganbaatar ◽  
O Tamir ◽  
E Lkhagvamaa ◽  
A Tsiiregzen ◽  
J Oyuntsetseg ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Antioxidative Activity ◽  
Antioxidant Properties ◽  
Milk Thistle ◽  
Dpph Assay

Silibum marianum (Milk thistle) contains high amount of phenolic compounds with antioxidant properties. The aim of this work was to evaluate the antioxidant activity of  phenolic compounds included in Milk thistle. The  antioxidant properties of the leaves and seeds of milk thistle were examined by determining its ability to scavenge the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical. The results obtained from DPPH assay confirm that Milk thistle extracts have high antioxidative activity.DOI: http://doi.dx.org/10.5564/mjc.v15i0.323 Mongolian Journal of Chemistry 15 (41), 2014, p53-55

scholarly journals Evaluation of Antioxidant Interactions of Combined Model Systems of Phenolics in the Presence of Sugars

2016 ◽  
Vol 11 (10) ◽  
pp. 1934578X1601101 ◽  
Author(s):  
Mirela Kopjar ◽  
Ante Lončarić ◽  
Mateja Mikulinjak ◽  
Žaklina Šrajbek ◽  
Mihaela Šrajbek ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Synergistic Effect ◽  
Gallic Acid ◽  
Synergistic Effects ◽  
Model System ◽  
Model Systems ◽  
Combined Model ◽  
Frap Assay ◽  
Antagonistic Effects ◽  
Acid Addition

The aim of this study was to determine the antioxidant activity of model systems of phenolics (individual and combinations) without and with the addition of sugars (sucrose or trehalose). Also, the synergistic or antagonistic effects of the phenolics in all model systems were evaluated. The results for the antioxidant activity were obtained by three assays, ABTS+•, DPPH• and FRAP. ABTS+• and DPPH• assay showed that quercetin and gallic acid had the same antioxidant activity, higher than catechin. When the FRAP assay was applied, the order of increasing of antioxidant activity was quercetin < gallic acid < catechin. The highest synergistic effect was achieved in the model system of catechin, quercetin and gallic acid. Addition of sugars, sucrose and trehalose, influenced both antioxidant activity and synergistic effect of phenolics in the model systems. The highest synergistic effect in the model systems with sucrose addition was observed with catechin, quercetin and gallic acid, while in samples with trehalose, the highest synergistic effect was in combination with catechin and gallic acid. Interactions of phenolics and phenolics/sugars caused changes in antioxidant activity as well as synergistic effects.

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