scholarly journals ANALYSIS OF MASS PORTIONS OF BIOGENIC ELEMENTS IN DENTAL TISSUE

2018 ◽  
Vol 9 (2) ◽  
Author(s):  
Irena Kuzmanović Radman ◽  
Aleksandra Đeri ◽  
Adriana Arbutina ◽  
Ognjenka Janković ◽  
Nataša Trtić ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Drinking Water ◽  
Biogenic Elements ◽  
Long Term Effects ◽  
Dental Tissue ◽  
Dental Tissues ◽  
Significant Difference ◽  
Potassium Iodine ◽  
Experimental Group ◽  
Experimentally Induced

Hard dental tissues represent ideal tissues for assessing the long-term effects of exposure to toxic metals. The aim of this paper was to determine the representation and the relation between mass portions of the following elements: carbon, oxygen, sodium, magnesium, aluminum, chlorine, potassium, iodine and lead in certain parts of the teeth (enamel-dentine line, dentine, pulp) with rats with experimentally induced diabetes mellitus (DM) by using SEM/EDS analysis, after 14 and 30 days of exposing animals to lead. Material and methods of work: The study was conducted in rats of Wistar strains divided into two groups. The first experimental group (A1) consisted of 8 rats, taking lead in the course of 14 days at a concentration of 1500 ppm and the second experimental group (A2) consisted of 8 rats taking lead in the course of 30 days at a concentration of 1500 ppm. The rats from group А1 and А2 had induced diabetes mellitus by using the Alloxan which was administered intraperitoneally at a dose of 100mg per kilogram of body weight. The teeth samples were analyzed by scanning electron microscopy (SEM). Results: No lead was detected in the teeth of rats with experimentally induced diabetes that received lead in drinking water in the course of 14 days, while the average values of mass portions of other examined elements amounted to: carbon -24,25 %, oxygen – 38,17%, sodium -0,9%, magnesium -0,11%, aluminum – 0,07%, chlorine – 0,21% and iodine – 0,32%. The average values of the mass portions of examined elements in the enamel of teeth of rats receiving lead in the course of 30 days amounted to: lead -0,36%, carbon-31,09%, oxygen – 41,13%, sodium – 0,91%, magnesium – 0,21%, chlorine – 0,22%, potassium – 0,03% and iodine – 0,17%. Conclusion: Mass portions of elements found in the teeth enamel of rats receiving lead in the course of 30 days with experimentally induced diabetes were higher but with no statistically significant difference compared to the mass portions of elements in the group of teeth of rats taking lead through drinking water in the course of 14 days with experimentally induced diabetes, too. The lead was detected in the teeth of rats that received lead for 30 days with induced diabetes but only in the enamel.

scholarly journals Expression of a fibronectin in the dental pulp of lead-intoxicated rats with experimentally-induced diabetes mellitus

2020 ◽  
pp. 102-102
Author(s):  
Irena Kuzmanovic-Radman ◽  
Aleksandra Djeri ◽  
Radoslav Gajanin ◽  
Adriana Arbutina ◽  
Renata Josipovic ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Drinking Water ◽  
Lead Exposure ◽  
Immunohistochemical Analysis ◽  
Control Group ◽  
Wistar Strain ◽  
Significant Difference ◽  
Fibronectin Expression ◽  
Diffuse Positivity ◽  
Experimentally Induced

Introduction/Objective. Lead exposure represents one of the most important factors that affect the general health, including oral health and it is associated with enamel and dentin tooth defects. The aim of this paper was to determine expression of the fibronectin in the pulp of rats with experimentally induced diabetes mellitus (DM), after lead exposure, by using immunohistochemical analysis. Methods. The study was conducted on 42 rats of Wistar strain. Intoxication of rats with lead-acetate was done via drinking water ad libitum. The first group (Exp_14) consisted of 16 rats, which received lead in water for 14 days, the second group (Exp_30) consisted of 16 rats which received lead in water for 30 days at the same concentration (1500 ppm), while the control consisted of 10 healthy rats. Groups Exp_14 and Exp_30 were induced into DM, by using the Alloxan intraperitoneally. Pathohistological and immunohistochemical analysis determined fibronectin expression in pulp, odontoblasts, predentin and dentine of the teeth. Results. High diffuse positivity of fibronectin in group Exp_14 was noticed in 63.6% of rats, in group Exp_30 in 24.0% of rats, while in the control group it was noticed in 50.0% of rats. There was no statistically significant difference in the expression of fibronectin between the examined groups. Conclusion. Lead intoxication through drinking water, for a period of 14 and 30 days, had effect on the expression of fibronectin in the pulp, odontoblasts, predentin and dentin of the teeth of animals experimentally induced DM.

Thyroid Dysfunction in Pregnant Women with Gestational Diabetes Mellitus

2020 ◽  
Vol 16 (8) ◽  
pp. 895-899 ◽  
Author(s):  
Shahin Safian ◽  
Farzaneh Esna-Ashari ◽  
Shiva Borzouei
Keyword(s):  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Pregnant Women ◽  
Thyroid Dysfunction ◽  
Control Group ◽  
Thyroid Peroxidase ◽  
Free T4 ◽  
Significant Difference ◽  
Experimental Group

Aims: Investigation thyroid dysfunction and autoimmunity in pregnant women with gestational diabetes mellitus. Background: This article was written to evaluate the thyroid function and anti-thyroid peroxidase (anti- TPO) antibodies in pregnant women with gestational diabetes mellitus (GDM). Method: A total of 252 women with GDM and 252 healthy pregnant women were enrolled. Thyroid tests, including TSH, FreeT3, Free T4, and anti-TPO were performed for all women at 24–28 weeks of gestation. Data analysis was then carried out using SPSS ver. 22. Result: There was a significant difference between the experimental group (38.4%) and the control group (14.06%) in terms of the prevalence of subclinical hypothyroidism (p= 0.016). The frequency of anti-TPO was higher in the experimental group than the control group and positive anti-TPO was observed in 18.6% of women with GDM and 10.3% of healthy pregnant women (P= 0.008). Conclusion: Thyroid disorders are observed in pregnant women with GDM more frequently than healthy individuals and it may be thus reasonable to perform thyroid tests routinely.

scholarly journals The Antioxidant Effect of Curcumin and Rutin on Oxidative Stress Biomarkers in Experimentally Induced Periodontitis in Hyperglycemic Wistar Rats

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1332
Author(s):  
Gilda M. Iova ◽  
Horia Calniceanu ◽  
Adelina Popa ◽  
Camelia A. Szuhanek ◽  
Olivia Marcu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Diabetic Rats ◽  
Gingival Tissue ◽  
Control Group ◽  
Albino Rats ◽  
Oxidative Stress Biomarkers ◽  
Significant Difference ◽  
The Impact ◽  
Experimentally Induced

Background: There is a growing interest in the correlation between antioxidants and periodontal disease. In this study, we aimed to investigate the effect of oxidative stress and the impact of two antioxidants, curcumin and rutin, respectively, in the etiopathology of experimentally induced periodontitis in diabetic rats. Methods: Fifty Wistar albino rats were randomly divided into five groups and were induced with diabetes mellitus and periodontitis: (1) (CONTROL)—control group, (2) (DPP)—experimentally induced diabetes mellitus and periodontitis, (3) (DPC)—experimentally induced diabetes mellitus and periodontitis treated with curcumin (C), (4) (DPR)—experimentally induced diabetes mellitus and periodontitis treated with rutin (R) and (5) (DPCR)—experimentally induced diabetes mellitus and periodontitis treated with C and R. We evaluated malondialdehyde (MDA) as a biomarker of oxidative stress and reduced glutathione (GSH), oxidized glutathione (GSSG), GSH/GSSG and catalase (CAT) as biomarkers of the antioxidant capacity in blood harvested from the animals we tested. The MDA levels and CAT activities were also evaluated in the gingival tissue. Results: The control group effect was statistically significantly different from any other groups, regardless of whether or not the treatment was applied. There was also a significant difference between the untreated group and the three treatment groups for variables MDA, GSH, GSSG, GSH/GSSG and CAT. There was no significant difference in the mean effect for the MDA, GSH, GSSG, GSH/GSSG and CAT variables in the treated groups of rats with curcumin, rutin and the combination of curcumin and rutin. Conclusions: The oral administration of curcumin and rutin, single or combined, could reduce the oxidative stress and enhance the antioxidant status in hyperglycemic periodontitis rats.

scholarly journals Effect of Diabetes Education Program on Gestational Diabetes Mellitus Knowledge Among Diabetic Pregnant Women: An Experimental Study

2020 ◽  
Author(s):  
Uchenna Cosmas Ugwu ◽  
Osmond Chukwuemeka Ene
Keyword(s):  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Pregnant Women ◽  
Education Program ◽  
Diabetes Education ◽  
Control Groups ◽  
Significant Difference ◽  
And Control ◽  
Experimental Group

Abstract Background: Gestational diabetes mellitus (GDM) is a disease condition present during pregnancy and if not properly managed would have severe effect on the maternal and neonatal health. This research determined the effect of diabetes education program (DEP) on gestational diabetes mellitus knowledge (GDMK) among diabetic pregnant women (DPW) and significant differences within groups.Methods: This study was based on a pretest – posttest measures of experimental research design involving experimental (n=110) and control (n=110) groups. A total of 220 DPW were purposively recruited from public hospitals in Nigeria between October and December 2019. The DEP was delivered to DPW in experimental group. The Gestational Diabetes Mellitus Knowledge Questionnaire (GDMKQ) of 0.774 was used for data collection. The statistical analysis was completed using IBM SPSS version 22. Results: The results revealed that no statistically significant difference existed on GDM history (P=0.801>0.05), smoking habit (P=0.0615>0.05), parity status (P=0.503>0.05), and level of education (P=0.720>0.05), while difference was observed on alcohol status (P=0.011<0.05), and age by birth (P=0.009<0.05). Also, while no significant difference existed between the DPW in the treatment and control groups on GDMK pretest measures (P=0.901>0.05), difference existed between the DPW in the intervention and control groups on GDMK posttest scores (P=0.026<0.05). Also, the higher percentage score (78%) of DPW in the experimental group in the posttest GDMK measures which is higher than the score in the pretest measures (55%) of the same group were indications that DEP has significant effect on GDMK among DPW.Conclusion: This demonstrates that reliable interventions are effective in combating pregnancy-related complications. This requires collaborative efforts by concerned bodies to combat notable complications during pregnancy using reliable intervention.Trial Registration: In retrospect, the Pan African Clinical Trial Registry documented the study with registration trial code (Trial No.: PACTR201903003187005)

scholarly journals Lead concentration in hard dental tissues – SEM/EDS analysis

2018 ◽  
Vol 65 (1) ◽  
pp. 14-21
Author(s):  
Irena Kuzmanović Radman ◽  
Adriana Arbutina ◽  
Renata Josipović ◽  
Aleksandra Đeri
Keyword(s):  
Mass Fraction ◽  
Human Organism ◽  
Control Group ◽  
Long Term Effects ◽  
Dental Tissues ◽  
Eds Analysis ◽  
Single Tooth ◽  
Hard Dental Tissues ◽  
Experimentally Induced

Summary Introduction Currently, one of the most important ecological issues is exposure to lead in environment, since it is a metal with evident toxic effects on human organism. Hard dental tissues are suitable structures for assessing long-term effects of exposure to toxic metals. The aim of this paper was to determine the concentration of lead in hard dental tissues of a rat with experimentally induced DM using SEM/EDS analysis, after 14 and 30 days of exposing animals to lead. Material and methods The study was conducted in rats of Wistar strains divided into the three groups. The first group consisted of 8 rats (128 molars and premolars of the upper and lower jaws) with experimentally induced DM, taking lead in the course of 14 days at the concentration of 1500 ppm; the second group included 8 rats (128 molars and premolars of the upper and lower jaws) taking lead in the course of 30 days at the concentration of 1500 ppm, while the third control group consisted of 5 healthy rats (80 molars and premolars of the upper and lower jaws). Experimental animals received lead-acetate every day at the concentration of 1500 ppm via water ad libitum. In these animals, diabetes mellitus was induced by Alloxan. The teeth samples were analysed using scanning electron microscopy (SEM). EDS analysis determined the mass fraction of lead and other elements in hard dental tissues. Results No lead was detected in a single tooth layer in the teeth of rats that received lead in drinking water in the course of 14 days. The average values of the mass fraction of lead, calcium, and phosphorus in enamel of teeth of rats receiving lead in the course of 30 days amounted to: lead 0.36%, calcium 15.48%, and phosphorus 10.62%. Lead was registered only in enamel. Conclusion Lead was detected in enamel only in rats receiving lead in the course of 30 days while it was not detected in teeth after the course of 14 days.

scholarly journals Penyembuhan Luka Grade 2 pada Pasien Diabetes Mellitus dengan Modern Dressing Wound Care

2020 ◽  
Vol 4 (1) ◽  
pp. 222-230
Author(s):  
I Dewa Ayu Rismayanti ◽  
I Made Sundayana ◽  
Putu Eka Pratama
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Standard Deviation ◽  
Wound Care ◽  
Statistical Tests ◽  
Control Group ◽  
P Value ◽  
Significant Difference ◽  
Post Test ◽  
Experimental Group

This study aims to determine the effect of modern dressings on wound healing of grade 2 diabetes mellitus at Griya Utami Care Bali. The design used in the study was a real experiment with a one-group pre-post test approach. The results showed that the experimental group's average post value was 8.67, with a standard deviation of 2.024. In the control group, the average post value was 10.60, with a standard deviation of 2.874. Based on the results of statistical tests, it was found that the p-value was 0.042, meaning that there was a significant difference in the average post value between the experimental group and the control group. In conclusion, there is an effect of modern dressings on wound healing for grade 2 diabetes mellitus at Griya Utami care Bali. Keywords: Diabetes Mellitus, Grade 2 Diabetes Wounds, Modern Dressing

scholarly journals Assessment of Family Focused Intervention on Knowledge among Diabetic Clients

2020 ◽  
Vol 5 (9) ◽  
pp. 620-625
Author(s):  
Manonmani K ◽  
Kanchana S
Keyword(s):  
Diabetes Mellitus ◽  
Structured Interview ◽  
Control Group ◽  
Research Approach ◽  
Foot Care ◽  
Significant Difference ◽  
Post Test ◽  
Level Of Knowledge ◽  
And Control ◽  
Experimental Group

Diabetes mellitus is one of the most common chronic disease worldwide. The low levels of education and poor awareness about the disease have an impact on the health of the people living with diabetes mellitus. To assess the need of family focused intervention on knowledge among diabetic clients. Research approach utilized was quantitative research approach. Research design followed was true experimental design. Family focused intervention was the independent variable in the study. Knowledge was the dependent variable. Samples were selected using total enumeration technique. Pre test was assessed using structured interview schedule. Family focused intervention was given after pre test which includes IEC, counseling regarding diet and specific management, demonstration regarding foot care and exercise. Post test was conducted using the same tool. Comparison of the pre and post test level of knowledge showed that the overall mean knowledge score in the post test was 24.50 and 10.29 in experimental and control group respectively. Comparison between experimental and control group by unpaired ‘t’ test, t=28.03 revealed that there was a high significant difference between the experimental and control group. The results showed that there was significant improvement in the level of knowledge only in the experimental group. This revealed that family focused intervention was effective among diabetic clients. Knowledge was improved among experimental group to whom intervention was given. Hence family focused intervention can be incorporated as an integral component of the comprehensive health care services at primary level to enable the diabetic clients to manage the condition and prevent the complications.

scholarly journals Research on the changes and significance of serum RBP4 level in patients with senior coronary heart disease accompanying diabetes mellitus

2019 ◽  
Vol 6 (1) ◽  
pp. 26
Author(s):  
Qinxue Li ◽  
Biao Ge ◽  
Ruijing Yan ◽  
Yinlong Bai ◽  
Yan Liu
Keyword(s):  
Diabetes Mellitus ◽  
Coronary Heart Disease ◽  
Heart Disease ◽  
Coronary Artery ◽  
Density Lipoprotein ◽  
Coronary Artery Lesion ◽  
Lipoprotein Cholesterol ◽  
Control Group ◽  
Significant Difference ◽  
Experimental Group

Objective: To explore the significance of serum Retinol binding protein 4 (RBP4) and its relationship with coronary artery lesion in patients with senior coronary heart disease accompanying T2DM by determining the level of serum RBP4 and Gensini score.Methods: 30 cases of patients who were 60 years old above with coronary heart disease accompanying T2DM were selected and included in the experimental group, and 30 cases of patients of 60 years old above with coronary heart disease alone were included in the control group. Both groups of patients were given CAG examinations. In addition, Gensini score was calculated according to different degrees and parts of coronary artery lesion. It was required to record each patient’s age, gender, fasting blood glucose (FPG), triglycerides (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), glycated hemoglobin (HbA1c) and other laboratory examination indexes. ELISA was used to detect the level of serum RBP4 in each group, and statistical analysis was performed to the data in each group.Results: (1) RBP4 level, GS score, FPG and LDL-C in the experimental group were all higher than those in the control group, and the difference was of statistical significance (p < .05). There was no statistically significant difference in age, gender, TC, TG and HDL-C between two groups. (2) RBP4 was positively correlated to FPG and HbA1c. (3) In patients with senior coronary heart disease accompanying diabetes mellitus, HbA1c was positively correlated to GS score, RBP4 and FPG. (4) RBP4 was a risk factor for coronary artery stenosis in patients with senior coronary heart disease accompanying diabetes mellitus.Conclusions: The level of serum RBP4 in patients with senior coronary heart disease accompanying diabetes mellitus is higher than that in patients with coronary heart disease alone, with a deeper degree of coronary artery lesion. The level of serum RBP4 is increased with the degree of lesion deepened in patients with senior coronary heart disease, suggesting that the level of serum RBP4 is expected to be an early predictor of coronary artery lesion for patients with senior coronary heart disease.

scholarly journals Distortion Product Otoacoustic Emissions (DPOAE) patterns in individuals with Type 1 and Type 2 Diabetes Mellitus - A comparative study

2021 ◽  
Vol 8 (3) ◽  
pp. 001-009
Author(s):  
Madhavan Balakrishnan ◽  
Jyothis Kurian ◽  
Prem G Nair
Keyword(s):  
Diabetes Mellitus ◽  
Outer Hair Cells ◽  
Control Group ◽  
Distortion Product ◽  
Type 2 Dm ◽  
Significant Difference ◽  
Group 2 ◽  
Experimental Group

Diabetes mellitus (DM) is a chronic and potentially life-threatening condition, incidence of which is increasing rapidly in the present era. Various studies showed conflicting relationship between DM and Hearing Loss (HL). The current study was carried out to find the effect of Type 1 and Type 2 DM on hearing. The study group consisted of 90 adults in the age range of 20 to 40 years from various hospitals in and around Calicut, Kerala. These participants were divided into three groups: Group1 (Experimental group 1), included 30 individuals with Type 1 DM; Group 2 (Experimental group 2), included 30 individuals with Type 2 DM and Group 3 (Control group) included 30 age-matched non-diabetic individuals with normal hearing sensitivity. Results of the study revealed that there was significant difference in the DPOAE amplitude between Type 1 and Type 2 DM with control group and there was no significant difference in the DPOAE amplitude between Type 1 and Type 2 DM group. From the results it can be concluded that DPOAE amplitude were reduced in both Type 1 and Type 2 DM when compared to control group. This could be attributed to damage of the cochlear Outer Hair Cells (OHCs). Further, it could be assumed that damage to the OHCs due to DM in both Type 1 and Type 2 groups are relatively equal.

scholarly journals ¬Long-Term Effects of CIPS-based Training Module on Professional Elementary School Teachers

2021 ◽  
Vol 10 (4) ◽  
Author(s):  
Erni Munastiwi
Keyword(s):  
Elementary School ◽  
Elementary School Teachers ◽  
Long Term Effects ◽  
Training Module ◽  
Control Groups ◽  
School Teachers ◽  
Significant Difference ◽  
And Control ◽  
Experimental Group

CIPS-based Training Module has been developed to encourage teachers to improve their personal skills. Hence, the aim of this study is to investigate the long-term effects of the CIPS-based Training Module on the elementary school teachers. This study used the quasi-experimental design that involved 63 elementary school teachers. The teachers were divided into the experimental and control groups to test the objective of this study. The indicators of data analysis used in this study are mean score, standard deviation and independent sample t-test. The results show that there is a significant difference between the experimental and control groups. The experimental group obtained a higher mean score than the control group, which indicates that the CIPS-based Training Module has a positive long-term effect on the elementary school teachers from the experimental group. Hence, it is recommended the CIPS-based Training Module be implemented in the training of teachers, as their thinking and teaching skills will improve, which in turn will benefit the students in attaining high achievement.

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