scholarly journals Purification, characterization and antioxidant activities in vitro of polysaccharides from Amaranthus hybridus L.

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9077
Author(s):  
Zizhong Tang ◽  
Caixia Zhou ◽  
Yi Cai ◽  
Yujia Tang ◽  
Wenjun Sun ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Structural Characteristics ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Monosaccharide Composition ◽  
In Vitro Test ◽  
Amaranthus Hybridus ◽  
Total Antioxidant

Background Amaranthus hybridus L. is an annual, erect or less commonly ascending herb that is a member of the Amaranthaceae family. Polysaccharides extracted from traditional Chinese medicines may be effective substances with antioxidant activity. Methods In this study, we isolated crude polysaccharides from A. hybridus (AHP-M) using microwave-assisted extraction. Then, the AHP-M was purified by chromatography with DEAE-32 cellulose, and two fractions, AHP-M-1 and AHP-M-2, were obtained. The structural characteristics of AHP-M-1 and AHP-M-2 were investigated, and their antioxidant activities were analyzed in vitro. Results We found that the monosaccharide composition of AHP-M-1 was different from that of AHP-M-2. The molecular weights of AHP-M-1 and AHP-M-2 were 77.625 kDa and 93.325 kDa, respectively. The results showed that the antioxidant activity of AHP-M-2 was better than that of AHP-M-1. For AHP-M-2, the DPPH radical scavenging rate at a concentration of 2 mg/mL was 78.87%, the hydroxyl radical scavenging rate was 39.34%, the superoxide anion radical scavenging rate was 80.2%, and the reduction ability of Fe3+ was approximately 0.90. The total antioxidant capacity per milligram of AHP-M-2 was 6.42, which was higher than that of Vitamin C (Vc). Conclusion The in vitro test indicated that AHP-M-1 and AHP-M-2 have good antioxidant activity, demonstrating that A. hybridus L. polysaccharide has immense potential as a natural antioxidants.

scholarly journals Antioxidant potential of some Sudanese medicinal plants used in traditional medicine

2015 ◽  
Vol 3 (2) ◽  
pp. 192 ◽  
Author(s):  
Hatil EL-Kamali ◽  
Sana E. M. Hamed
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Punica Granatum ◽  
In Vitro Models ◽  
Water Extracts ◽  
Total Antioxidant ◽  
Sudanese Medicinal Plants

<p>In the present work methanolic and water extracts of 19 medicinal plants, commonly used in Sudanese folk medicines against gastrointestinal tract, infectious diseases were investigated for their antioxidant activity. Antioxidant assays were carried out by using different in vitro models such as DPPH (2, 2-diphenyl-1picrylhydrazyl) radical scavenging and Iron chelating activity. Methanolic and water extracts of <em>Punica granatum </em>showed the highest total antioxidant activity of 71±0.03 and 73±0.01 respectively. The antioxidant activities of water extracts were poor when compared to the methanol extract. The iron chelating power of methanol and aqueous extracts of <em>Commiphora myrrha</em> 58±0.01 and <em>Azadirachta indica</em> 57±0.20 respectively.</p>

scholarly journals Extraction of polysaccharides from Amaranthus hybridus L. by hot water and analysis of their antioxidant activity

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7149 ◽  
Author(s):  
Yujia Tang ◽  
Yirong Xiao ◽  
Zizhong Tang ◽  
Weiqiong Jin ◽  
Yinsheng Wang ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Economic Value ◽  
Radical Scavenging ◽  
Hot Water ◽  
Amaranthus Hybridus ◽  
Diethylaminoethyl Cellulose ◽  
Acidic Polysaccharides ◽  
Total Antioxidant ◽  
Effective Use

Background Amaranthus hybridus L. is an annual herb that belongs to the Amaranthceae family, a type of multi-purpose grain, vegetable and feed crop that has received considerable attention due to its great economic value. However, the composition of polysaccharides from A. hybridus has rarely been previously reported. Methods In this study, the aboveground part of A. hybridus was used as material and polysaccharides were isolated by the hot water extraction method. Two acidic polysaccharides were isolated and purified by the Sevage method and diethylaminoethyl cellulose-32 column chromatography. Results Two acidic polysaccharides were obtained from A. hybridus: AHP-H-1 and AHP-H-2. There were significant differences between the monosaccharide content from each sample according to gas chromatography-mass spectrometer. AHP-H-2 had higher antioxidant activity in vitro than AHP-H-1. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate of two mg/mL AHP-H-2 was 80%, its hydroxyl radical scavenging rate was approximately 48.5%, its superoxide anion radical scavenging rate was 85.3% and its reduction ability of Fe3+ was approximately 0.92. The total antioxidant capacity of each milligram of AHP-H-2 was 6.5, which was higher than ascorbic acid. Conclusion The results of the study promote the effective use of A. hybridus and provide a theoretical basis for its development.

scholarly journals QUANTITATIVE ANALYSIS OF NATURAL ANTIOXIDANTS AND FREE RADICAL SCAVENGING ACTIVITIES OF CRUCIFERAE VEGETABLES

2017 ◽  
Vol 10 (4) ◽  
pp. 470
Author(s):  
Jamuna K S ◽  
Ramesh C K ◽  
Aditya Rao Sj ◽  
Paramesha M ◽  
Riaz Mahmood
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Extraction Procedure ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Plant Materials ◽  
Cruciferous Vegetable ◽  
The Relationship ◽  
In Vitro Antioxidant Activity

Objective: The aim of this research program is to evaluate the antioxidant potentialities comprehensively utilizing commonly available cruciferous vegetables of India, viz., cabbage, cauliflower, kohlrabi, and radish.Methods: The plant materials were procured and processed for cold extraction procedure using 70% ethanol. The extracts were primarily assessedfor their phytoconstituents and further for their in vitro antioxidant activity using various qualitative and quantitative estimations. The results of quantitative estimations were expressed in terms of equivalence with respective standards, and the antioxidant potentiality of various scavenging and chelating activities were expressed in terms of 50% effective concentration. Further, correlation studies were made between quantitative and qualitative assays to study the relationship between the effects of different phytoconstituents groups.Results: In this study, the results revealed that all the four cruciferous vegetable extracts possess potential antioxidant activities. Among the extractsunder study kohlrabi has recorded superior antioxidant potential than others. It is clear from the study that the tested cruciferous vegetablesmanifested differential expression of antioxidant capacity due to their phytoconstituents.Conclusion: From the results, it can be concluded that the Cruciferae vegetables have potent antioxidant activity contributing to the use for healthbenefits in addition to their nutritive role as vegetable.Keywords: Cruciferae vegetables, Phytochemicals, In vitro antioxidant activity, Correlation.

CHEMO-PROFILING AND ASSESSMENT OF IN VITRO ANTIOXIDANT EFFICACY OF EIGHT FERNS OF DARJEELING HIMALAYAS, INDIA

2021 ◽  
pp. 25-27
Author(s):  
Nishika Jaishee ◽  
Rohini Lama ◽  
Usha Chakraborty
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Natural Antioxidants ◽  
Phenolic Contents ◽  
Darjeeling Himalayas

AIM: The present study was performed to prole some phenolics and explore the antioxidant effect of eight locally available ferns collected from different places of Darjeeling Himalayas, India. Methods: The antioxidant activities of methanol (MeOH) extract was evaluated by DPPH free radical scavenging activity. Qualitative analysis of phenol was done using standard methods. Further, characterization of phenolics was done using High performance liquid chromatography. Result: The content of phenolics ranged from 6.77 to 60.066mg FAE/g dry weight. The DPPH antioxidant activity expressed as IC values 50 revealed Nephrolepis cordifolia and Microsorum punctatum to exhibit highest and lowest antioxidative activity respectively. Moderate correlation 2 (R =0.547) was observed between the total phenolics content and antioxidant activity. HPLC analysis of phenolics from all the investigated plants revealed the presence of caffeic acid, ferulic acid and salicylic acid while the other phenolics such as phloroglucinol, gallic acid, pyrogallol, 3,4- dihy droxybenzoic acid, catechol, catechin, chlorogenic acid, caffeine, vanillic acid and cinnamic acid were not uniformly present in all the plants. The phenolic contents values showed wide variation among themselves, as well as within different plants. These ferns with considerable amount of phenolics can be the potential source of natural antioxidants.

scholarly journals EFFECT OF TEMPERATURES EXTRACTION ON IN VITRO ANTIOXIDANT ACTIVITIES OF POLYSACCHARIDES FROM OPHIOCORDYCEPS SOBOLIFERA

2019 ◽  
Vol 128 (1D) ◽  
Author(s):  
Khoa Van Tran ◽  
Hieu Trung Le ◽  
Son Lam Le ◽  
Vu Xuan Anh Ho ◽  
Kiet Tam Trinh ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Extraction Temperature ◽  
Total Antioxidant ◽  
Value Range ◽  
Ophiocordyceps Sobolifera ◽  
Dpph Radical Scavenging

<p>The objective of the work reported in this paper was to evaluated effects of temperatures extraction on <em>in vitro</em> antioxidant activities of polysaccharides from the <em>Ophiocordyceps sobolifera. </em>The antioxidant capacity of <em>Ophiocordyceps sobolifera </em>polysaccharide was evaluated to use  2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and total antioxidant activity methods. From this study, it could be concluded that the highest antioxidant activity of the polysaccharides could be obtained from extraction temperature 80 <sup>o</sup>C. Total antioxidant capacity of polysaccharides from <em>Ophiocordyceps sobolifera </em>  contained from 0.1183 ± 0.0011 to 0.1454 ± 0.0021 mg GA/g or from 0.1105 ± 0.0004 to 0.1215 ± 0.0006 μmol AS/g. Polysaccharides has the most potential antioxidant activity with the lowest half maximal inhibitory concentration (IC<sub>50</sub>) value range 0.97 mg/mL to 1.06 mg/mL.</p><p> </p>

scholarly journals Phenolic and Flavonoid Content and Antioxidant Activity of Daphne Blagayana Growing in Serbia

2014 ◽  
Vol 15 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Nedeljko Manojlovic ◽  
Miroslav Sovrlic ◽  
Pavle Maskovic ◽  
Perica Vasiljevic ◽  
Marina Juskovic
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Total Antioxidant ◽  
First Time ◽  
Phenolic And Flavonoid

ABSTRACT Th e aim of this study was to examine the phenolic and flavonoid contents and antioxidant activities of methanol and chloroform extracts of leaves and twigs of Daphne blagayana. Th e total phenolic content in the chloroform extract of plant twigs (90.26 ± 0.69 mg GA/g) was higher than that of the other extracts (from 76.56±0.89 to 77.45±0.43 mg GA/g). In the case of flavonoids, a greater value was also obtained for the chloroform extract of twigs (35.24±0.55 mg RU/g). Several diff erent methods were used to determine the antioxidant activity of the tested extracts, including total antioxidant capacity, metal chelating activity, hydroxyl radical scavenging activity and inhibitory activity against lipid peroxidation. Our results showed that although secondary metabolites of the plants may contribute significantly to their antioxidant activities, those antioxidant activities were not directly related to the phenolic and flavonoid amounts. Th e results of the present analysis demonstrated, for the first time, that Daphne blagayana leaves and twigs possess high phenolic and flavonoid contents, as well as potential antioxidant activity. Th is study suggests that Daphne blagayana twigs and leaves may potentially be used as an accessible source of natural antioxidants.

scholarly journals Optimization of sunflower head pectin extraction by ammonium oxalate and the effect of drying conditions on properties

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xuemei Ma ◽  
Jiayi Yu ◽  
Jing Jing ◽  
Qian Zhao ◽  
Liyong Ren ◽  
...  
Keyword(s):  
High Performance ◽  
Freeze Drying ◽  
Antioxidant Activities ◽  
Structural Characteristics ◽  
Radical Scavenging ◽  
Ammonium Oxalate ◽  
Extraction Process ◽  
Size Exclusion ◽  
Pharmaceutical Industries

AbstractPectin is a kind of natural and complex carbohydrates which is extensively used in food, chemical, cosmetic, and pharmaceutical industries. Fresh sunflower (Helianthus annuus L.) heads were utilized as a novel source of pectin extracted by ammonium oxalate. The conditions of the extraction process were optimized implementing the response surface methodology. Under optimal extraction parameters (extraction time 1.34 h, liquid–solid ratio 15:1 mL/g, ammonium oxalate concentration 0.76% (w/v)), the maximum experimental yield was 7.36%. The effect of spray-drying and freeze-drying on the physiochemical properties, structural characteristics, and antioxidant activities was investigated by FT-IR spectroscopy, high performance size exclusion chromatography, and X-ray diffraction. The results showed freeze-drying lead to decrease in galacturonic acid (GalA) content (76.2%), molecular weight (Mw 316 kDa), and crystallinity. The antioxidant activities of pectin were investigated utilizing the in-vitro DPPH and ABTS radical-scavenging systems. This study provided a novel and efficient extraction method of sunflower pectin, and confirmed that different drying processes had an effect on the structure and properties of pectin.

In Vitro Antioxidant Activity Metaplexis Japonica Makino Extract and Derivatives

2014 ◽  
Vol 955-959 ◽  
pp. 387-389 ◽  
Author(s):  
Bao Qing Wang
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Acid Test ◽  
Acetone Extracts ◽  
Β Carotene ◽  
Ethyl Acetate Extracts

Antioxidant activities of acetone and ethyl acetate extracts from Metaplexis japonica Makino, one of famous medicine plants in the eastnorth region of China, named luomo in Chinese, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In DPPH, the antioxidant activity of the acetone extracts, ethyl acetate extracts and derivative were IC50 were 313.21, 266.92 and 118.78μg/mL, respectively. In the β-carotene-linoleic acid test, IC50 were 285.09, 351.57 and 123.89μg/mL. It was concluded that Metaplexis japonica Makino and its derivatives might be a potential natural source of antioxidants .

ASSESSMENT OF PHYTOCHEMICALS, TOTAL PHENOL, FLAVONOID CONTENT AND IN VITRO ANTIOXIDANT PROPERTY OF LARGE CARDAMOM EXTRACTS

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (10) ◽  
pp. 34-41
Author(s):  
Kanthlal S. K. ◽  
Jipnomon Joseph ◽  
Bindhu P. Paul ◽  
Vijayakumar M ◽  
Rema Shree A. B. ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Property ◽  
Bioactive Constituents ◽  
Preliminary Assessment ◽  
Reducing Power Assay ◽  
Large Cardamom

Amomum subulatum, commonly known as large or black cardamom, is a commonly used spice in Indian kitchens and is traditionally used to treat various ailments. To add more knowledge about the medicinal values of the fruit, this study was conducted to evaluate the in vitro antioxidant activities of aqueous, methanol, ethanol, hydro alcohol, ethyl acetate, acetone and chloroform extracts of the fruit. Preliminary assessment was done to detect the presence of phytoconstituents using identification tests. The antioxidant activity was measured by employing methods such as diphenylpicrylhydrazyl (DPPH) radical scavenging assay, total antioxidant activity equivalent to ascorbic acid, reducing power assay and superoxide anion scavenging assay. The antioxidant activities were compared with their respective phenol and flavonoid contents. Preliminary assessment revealed that large cardamom fruit is a good source of all the bioactive constituents as well as phenol and flavonoid essential for medicinal values. The extract obtained by polar solvents showed the highest antioxidant efficacy in relation to its phenol content. Also, all the solvent-soluble fractions showed a concentration-dependent antioxidant effect. Results from our study prove that large cardamom can alleviate oxidative stress, suggesting the potential of large cardamom as a functional food

scholarly journals An Overview of Structural Aspects and Health Beneficial Effects of Antioxidant Oligosaccharides

2020 ◽  
Vol 26 (16) ◽  
pp. 1759-1777 ◽  
Author(s):  
Tatiane F. Vieira ◽  
Rúbia C. G. Corrêa ◽  
Rosely A. Peralta ◽  
Regina F. Peralta-Muniz-Moreira ◽  
Adelar Bracht ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Sugar Content ◽  
Animal Health ◽  
Monosaccharide Composition ◽  
Degree Of Polymerization ◽  
Chemical Diversity ◽  
In Vivo Studies

Background: Non-digestible oligosaccharides are versatile sources of chemical diversity, well known for their prebiotic actions, found naturally in plants or produced by chemical or enzymatic synthesis or by hydrolysis of polysaccharides. Compared to polyphenols or even polysaccharides, the antioxidant potential of oligosaccharides is still unexplored. The aim of the present work was to provide an up-to-date, broad and critical contribution on the topic of antioxidant oligosaccharides. Methods: The search was performed by crossing the words oligosaccharides and antioxidant. Whenever possible, attempts at establishing correlations between chemical structure and antioxidant activity were undertaken. Results: The most representative in vitro and in vivo studies were compiled in two tables. Chitooligosaccharides and xylooligosaccharides and their derivatives were the most studied up to now. The antioxidant activities of oligosaccharides depend on the degree of polymerization and the method used for depolymerization. Other factors influencing the antioxidant strength are solubility, monosaccharide composition, the type of glycosidic linkages of the side chains, molecular weight, reducing sugar content, the presence of phenolic groups such as ferulic acid, and the presence of uronic acid, among others. Modification of the antioxidant capacity of oligosaccharides has been achieved by adding diverse organic groups to their structures, thus increasing also the spectrum of potentially useful molecules. Conclusion: A great amount of high-quality evidence has been accumulating during the last decade in support of a meaningful antioxidant activity of oligosaccharides and derivatives. Ingestion of antioxidant oligosaccharides can be visualized as beneficial to human and animal health.

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