scholarly journals Pith Necrosis of Tomato Caused by Pseudomonas viridiflava May Not Decrease Production

2019 ◽  
pp. 1-6
Author(s):  
F. P. Monteiro ◽  
C. Ogoshi ◽  
D. A. Cardoso ◽  
V. Perazolli
Keyword(s):  
Agricultural Research ◽  
Petri Dish ◽  
Adventitious Root Formation ◽  
First Year ◽  
Tomato Plants ◽  
Randomized Design ◽  
Pseudomonas Viridiflava ◽  
Etiological Agents ◽  
Pith Necrosis ◽  
Design Experiments

Aims: This study aimed to measure the losses in the production of tomato plants caused by the bacteria P. viridiflava. Study Design: Experiments were performed in a completely randomized design with six replicates. Place and Duration of Study: The study was conducted on the Caçador experimental station of the Agricultural Research and Rural Extension Enterprise of Santa Catarina (EPAGRI) from October to April during 2017/2018 and 2018/2019 crop season. Methodology: Bacteria were isolated from tomato plants with pith necrosis symptoms, using nutrient agar. The isolated strain was identified by a scheme of tests for bacteria that emit fluorescence, known as LOPAT, and by sequencing the 16S rDNA region. Tomato plants were cultivated for two seasons during 2017/2018 and 2018/2019. In the first year the cultivar Paronset was cultivated and in the next season the experiment was performed with the cultivars Compack, Nagai, Paronset and Pizzadoro. During the production season, tomato fruits were harvested and the weight was accounted for. At the beginning of the first bunch formation, the stems of the plant were inoculated with wood sticks containing bacterial colonies removed from a 48h-Petri dish culture medium. Tomato plants cultivated as control treatments were not inoculated. At the end of the cultivation seasons, the stems were cut to analyze the pith necrosis progress. Results: In both cultivation seasons, there was no decrease in the production associated with the pith necrosis caused by P. viridiflava EPAGRI BacPvT1 because the total weight of fruits harvested from inoculated plants was not statistically different compared to the non-inoculated plants. The disease progressed in all inoculated plants and adventitious root formation as external symptoms was observed. Conclusion: The bacteria Pseudomonas viridiflava EPAGRI BacPvT1, one of the etiological agents of pith necrosis of tomato, may not decrease the production. Even causing some injuries, it may be not cause any damage.

scholarly journals Envisioning the atmospheric effect through (im)materiality

2019 ◽  
Vol 64 ◽  
pp. 02007
Author(s):  
Nilay Ünsal Gülmez ◽  
Dürnev Atılgan Yagan ◽  
Murat Şahin ◽  
Efsun Ekenyazıcı Güney ◽  
Hande Tulum
Keyword(s):  
Interior Design ◽  
Analytical Method ◽  
Design Studio ◽  
First Year ◽  
Second Phase ◽  
Design Practice ◽  
Atmospheric Effect ◽  
Two Phases ◽  
Light Color ◽  
Design Experiments

In an attempt to bridge the gap between architectural/interior design practice and education, ‘atmosphere’ as a prolific contemporary architectural debate in practice and theory is covered by the experiment of ‘Staging Poe’ carried out as a first year Design Studio through the study of Edgar Allen Poe’s selected poems. Poe’s 1846 text of ‘The Philosophy of Composition’, unfolding his analytical method of writing and emphasis on “effect” in poetry, provides a ground for experimenting with facets of materiality and structuring the studio. Aiming to cultivate intuitive design experiments of students into informed processes in hybridizing conceptual/textual and material/sensual aspects, studio is structured in two phases. In the first phase, “materialization”, idiosyncratic interpretations of students from words to materials with a focus on tectonic experiments and haptic experiences are sought in between materializing and dematerializing processes. In the second phase, the “atmospheric”, emphasis on dematerialization of the perception of materials through tools, such as light, color and sound is exercised to transform the object into a performance stage. Outcomes of the studio on aspects pertaining to material and materialities in creation of the immaterial that is the atmosphere is followed and evaluated through responses of students’ weekly reports.

Physician Burnout and the Electronic Health Record Leading up to and During The First Year of COVID-19: A Systematic Review (Preprint)

2022 ◽  
Author(s):  
Clemens Scott Kruse ◽  
Michael Mileski ◽  
Gevin Dray ◽  
Zakia Johnson ◽  
Cameron Shaw ◽  
...  
Keyword(s):  
Empirical Evidence ◽  
Human Subjects ◽  
First Year ◽  
Physician Burnout ◽  
Life Balance ◽  
Loss Of Autonomy ◽  
Lack Of Control ◽  
Two Factors ◽  
The Hours ◽  
Design Experiments

BACKGROUND Physician burnout was first identified in 1974, and it has been particularly prevalent during the first year of the pandemic. OBJECTIVE To objectively analyze the literature over the last five years for empirical evidence of burnout incident to the EHR and to identify barriers, facilitators, associated patient satisfaction to using the EHR to improve symptoms of burnout. METHODS No human subjects were used in this review, however 100% of participants in studies analyzed were adult physicians. Four research databases and one targeted journal were queried for studies commensurate with the objective statement from January 1, 2016 through January 31st 2021 (n=25). RESULTS The hours spent in documentation and workflow are responsible for the sense of loss of autonomy, lack of work-life balance, lack of control of one’s schedule, cognitive fatigue, a general loss of autonomy and poor relationships with colleagues. Researchers have identified training, local customization of templates and workflow, and the use of scribes to alleviate the administrative burden of the EHR and decreased symptoms of burnout. CONCLUSIONS The solutions provided in the literature only addressed two of the three factors, workflow and documentation time, but not the third, usability. Practitioners and administrators should focus on the former two factors because they are within their sphere of control. EHR vendors should focus on empirical evidence to identify usability features with the greatest impact to improve. Researchers should design experiments to explore solutions that address all three factors of the EHR that contribute to burnout. INTERNATIONAL REGISTERED REPORT RR2-10.2196/15490

scholarly journals Sourcebook update: intestinal smooth muscle contractility and autonomic control

2018 ◽  
Vol 42 (2) ◽  
pp. 311-320 ◽  
Author(s):  
Philip D. Langton
Keyword(s):  
First Year ◽  
Smooth Muscle Contractility ◽  
Gut Motility ◽  
First Year Students ◽  
Authentic Inquiry ◽  
Student Teams ◽  
Functional Antagonism ◽  
The Individual ◽  
Design Experiments

This laboratory practical requires first-year students to anticipate the effects of drugs active at cholinergic and adrenergic receptors on gut motility in order to design experiments during an authentic inquiry exercise. Rather than specifying a strict sequence of drug additions that aim to provide ideal demonstrations of pharmacological and physiological antagonism, I have instead designed switches into the drugs provided and set students, working in small teams, the task of identifying the switched drugs, an inquiry activity. To extend the teamwork aspect, laboratory reports were submitted by the student teams rather than individual students. Staff observed that discussions within the teams were stimulated by the inquiry-led nature of the practical. The quality of the laboratory reports submitted by teams were substantially improved over the individual reports submitted in previous years. (Students previously worked in teams, but simply followed a list of prescribed experiments and wrote individual reports.) Although, in conversation, teams of students had an improved understanding of the regulation of gut motility by the parasympathetic and sympathetic divisions of the autonomic nervous system and could readily distinguish between pharmacological and functional antagonism, no attempt was made to evaluate learning because the revision was triggered by the observed effect of a technical error and was not otherwise planned. It is likely that laboratory practicals, in general, would benefit from inclusion of inquiry.

Attempts at Biological Control of Clavibacter michiganensis subsp. michiganensis On Rockwool-Grown Greenhouse Tomatoes

2008 ◽  
Vol 69 (1) ◽  
pp. 125-134 ◽  
Author(s):  
Czesław Ślusarski
Keyword(s):  
Biological Control ◽  
Biocontrol Agent ◽  
Root Zone ◽  
First Year ◽  
Bacterial Canker ◽  
Clavibacter Michiganensis ◽  
Tomato Plants ◽  
Clavibacter Michiganensis Subsp ◽  
Greenhouse Tomatoes ◽  
Bacterial Canker Of Tomato

Attempts at Biological Control ofClavibacter michiganensissubsp.michiganensisOn Rockwool-Grown Greenhouse TomatoesTwo greenhouse experiments were conducted in which tomato plants artificially inoculated withClavibacter michiganensissubsp.michiganensis(Cmm) were grown in an open rockwool system as spring and autumn crops. Two isolates of the rhizosphere bacteria,Pseudomonas fluorescensstrain PSR21,Pseudomonas reactansstrain GGS14, a commercial biocontrol agent Aqua Bac Plus (Bacillusspp.) and a proprietary disinfectant containing QAC+Chx, applied at weekly intervals, were evaluated for their efficiency in the suppression of the bacterial canker of tomato. All treatments tested revealed to be ineffective in controlling the disease. The introduction ofCmmbacteria into the fresh rockwool in the first year of its usage resulted in a 100% death of tomato plants, whereas following an artificial inoculation of two- and three-year-old rockwool slabs withCmmbacteria dead plants amounted to 70 and 58%, respectively. This indicates that in the re-used rockwool a natural microbial suppressiveness to bacterial canker of tomato might be developed in the root zone.

scholarly journals The Effect of Guano Walet Fertilizer and Ratu Biogen Foliar Fertilizer on the Growth and Yield of Tomato (Lycopercicum esculentum Mill.) Monza Variety

AGRIFOR ◽  
2018 ◽  
Vol 17 (2) ◽  
pp. 231
Author(s):  
Dian Kristina ◽  
Abdul Rahmi
Keyword(s):  
Plant Height ◽  
Fertilizer Application ◽  
Fruit Weight ◽  
Factorial Experiment ◽  
Growth And Yield ◽  
Tomato Plants ◽  
Foliar Fertilizer ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Village

This experiment aims to: (1) to study of the effect of guano walet fertilizer and Ratu Biogen foliar fertilizeras well as their interaction on the growth and yield of tomato plants; and (2) to find proper dosage of guano walet fertilizer and proper concentration of Ratu Biogen foliar fertilizer for better growth and yield of tomato plants.The research carried out from May 2014 to July 2014, in the Village Melak Ulu RT.20 Subdistrict Melak, West Kutai. It applied Completely Randomized Design with factorial experiment 4 x 4 and five replications.  The first factor is the dosage of the guano walet fertilizer (G) consists of 4 levels, namely: no fertilizer application guano walet (g0), 10 Mg ha ̵ ¹, or 100 g of polybag ̵ ¹ (g1), 15 Mg ha ̵ ¹ or 150 g polibag ̵ ¹ (g2), 20 Mg ha ̵ ¹ or 200 g polybag ̵ ¹   (g3). The second factor is the concentration of Ratu Biogen (B) consists of 4 levels: without POC Ratu Biogen (b0), 1 ml 1 ̵ ¹ water (b1), 2 ml 1 ̵ ¹ water (b2), 3 ml 1 ̵ ¹  water (b3).Result of the research revealed that : (1) application of guano walet fertilizer affect very significantly on plant height at 14, 28, 42 days after planting, the number of fruits per plant, and weight of fruit per plant, but the effect is not significant on the days of plant flowered and days of plant harvest.  The best production is attained by the 200 g polybag-1 fertilizer guano walet (g3), namely 282,50 plant-1, In reverse, the least production is attained by without fertilizer guano walet (g0), namely 227,25 g plant ̵ ¹; (2) application of Ratu Biogen foliar fertilizer after significantly to very significantly on the plant height at 14 days after planting  and the number of fruits per plant, but the effect is no significant on the plant height at 28 and 42 days after planting, days of plant flowered, days of plant harvest, and weight of fruit per plant; and (3) interaction between guano walet fertilizer and Ratu Biogen foliar fertilizer no significantly on the plant height at 14, 28, and 42 days after planting, days of plant flowered, days of plant harvest, number of fruit per plant, and fruit weight per plant.

scholarly journals Selection and Inheritance of Tomato Resistance against Ralstonia solanacearum

2019 ◽  
Vol 23 (1) ◽  
pp. 61
Author(s):  
Isna Maulida ◽  
Rudi Hari Murti ◽  
Triwidodo Arwiyanto
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Dominant Gene ◽  
Tomato Plants ◽  
Breeding Lines ◽  
Back Cross ◽  
Inheritance Patterns ◽  
Randomized Design ◽  
Resistant Varieties ◽  
Resistant Lines

Ralstonia solanacearum is a plant pathogen causes wilting which is a major obstacle in the cultivation of tomato plants. In plant breeding, knowledge of the source of resistance genes and inheritance patterns is important in the development of bacterial wilt resistant varieties. This study aimed to obtain bacterial wilt resistant lines and to find out the inheritance pattern of tomato resistance to bacterial wilt. Selection of resistant plant involved the selected breeding lines from irradiation and crossing collections of the Genetic Laboratory, Faculty of Agriculture, Universitas Gadjah Mada. Introduced lines of H-7996 and F1 Permata and Timoti were used as a control. H-7996 as resistant parents and GM2 as susceptible parents, and their offspring include F1 GM2 x H-7996, F1 reciprocal, F2, Back Cross 1 (F1 x GM2), and Back Cross 2 (F1 x H-7996) used in testing inheritance patterns. Inoculation was carried out 1 week after planting by pouring 100 ml of water suspension of R. solanacarum (108  cfu/ml) on the roots. Completely Randomized Design (CRD) was used in this experiment. The scoring observation was carried out every week for one month. This study showed that Permata as a control was the most resistant, while Timoti and H-7996 were medium resistant. The CLN, G6, G8, and G7 lines were susceptible medium, yet only G8 and G7 with the smallest percentage of disease intensity and not significantly different than Timoti. The resistance gene to bacterial wilt on H-7996 was controlled by genes in the cell nucleus with additive-dominant gene action. Resistance to bacteria has a moderate level of heritability.

scholarly journals Responses of Tomatoes Grafting Using Variation of Rootstock against Virus Infection and Tomato Yields

2020 ◽  
Vol 24 (1) ◽  
pp. 98
Author(s):  
Tri Retno Widyastuti ◽  
Sri Sulandari ◽  
Sedyo Hartono ◽  
Triwidodo Arwiyanto
Keyword(s):  
Viral Infection ◽  
Viral Infections ◽  
Field Research ◽  
Viral Diseases ◽  
Specific Primer ◽  
Resistance Response ◽  
Tomato Production ◽  
Tomato Plants ◽  
Randomized Design ◽  
Tomato Chlorosis Virus

Grafting methods on tomato have been done to reduce the infection rate of various pathogens. Begomovirus and Crinivirus are important viruses in tomato plants. The research aimed to determine the resistance response of tomato plants to viral infection, and tomato production. Field research was conducted in Harjobinangun, Pakem, Sleman, Yogyakarta in the endemic area of the viral diseases transmitted by Bemisia tabaci. This experiment used a Completely Randomized Design non-factorial with “Servo” as scion and “Amelia”, “H-7996”, “Mawar” as rootstock. The disease development, presence of viral diseases, and tomato yields were observed. PCR detection using Krusty & Hommr primer successfully amplified Begomovirus DNA bands with an approximate size of 580 bp in tomato plant with interveinal chlorosis, curling, thick, rigid, and stunt symptoms. Chlorotic spots and yellowing symptoms successfully amplified using ToCV-CF/ToCV-CR specific primer for the amplification of Tomato chlorosis virus with DNA band approximately size of 360 bp, whereas using TICV-CF/TICV-CR specific primer could not amplify the virus cDNA. The leaves roll upward with purple interveinal symptoms that were not infected by both viruses. Both viral infections affected the quality of the fruit which indicated by a higher number of abnormal fruits. “Servo” grafted onto “Amelia” and non-grafted Servo were tolerant to viral infection, “Servo” grafted onto “H-7996” or to “Mawar variety were susceptible to viral infection, self-grafted Servo were very susceptible to viral infection. 

Phosphite-based Products in the In vitro Colletotrichum musae Control

2019 ◽  
pp. 1-9
Author(s):  
Maria Luísa Mendes Rodrigues ◽  
Edson Hiydu Mizobutsi ◽  
Paola Junayra Lima Prates ◽  
Paula Virgínia Leite Duarte ◽  
Regina Cássia Ferreira Ribeiro ◽  
...  
Keyword(s):  
Mycelial Growth ◽  
Culture Medium ◽  
Culture Media ◽  
Petri Dish ◽  
State University ◽  
Randomized Design ◽  
Colletotrichum Musae ◽  
Study Laboratory ◽  
Vitro Effect

Aims: The aim of this study was to evaluate the in vitro effect of different phosphite formulations and concentrations on the development of Colletotrichum musae. Sample: to evaluate the inhibition of germination, mycelial growth and sporulation of Colletotrichum musae. Study Design:  Treatments were conducted in a completely randomized design, with 4 replicates, each replicate consisting of 1 Petri dish. Place and Duration of Study:  Laboratory of Post-Harvest Pathology, State University of Montes Claros, between March and October 2017. Methodology: Three different phosphite formulations were used: FCu1 (4% Cu + 20% P2O5), FCu2 (4% Cu + 22% P2O5) at concentrations of 0.5;1.0; 1.5 and 2.0 mL L-1 and FK (42% P2O5 + 27.7% K2O) at concentrations of 0.5; 1.0; 1.5 and 2.0 mg.L-1. Products were incorporated into the respective culture media. Culture medium alone and culture medium + imazalil were used as controls. Petri dishes were housed in BOD chamber at 25°C under a 12 hours photoperiod. Results: Results were submitted to analysis of variance and regression, and means were compared by the Tukey test (P <0.05). Control was compared to the other treatments by the Dunnet's test (P <0.05). Among the tested phosphite formulations, copper and potassium phosphites were found to reduce the mycelial growth of Colletotrichum musae. FCu2 presents a fungicide-like effect from the concentration of 0.5 m.L-1 in the control of conidia production. As for the FCu1, a fungicide-like effect was observed in the control of germination from the concentration of 1.5 mL.L-1. Conclusion: A significant fungistatic effect was observed between the concentrations of the products in the mycelial growth, sporulation and germination obtaining control of up to 100% of the development of C. musae. Copper phosphites were as effective as fungicide in inhibiting fungal development.

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