scholarly journals Bacterial Flora and Proximate Composition of Edible Frogs (Ptychadena mascareniensis and Ptychadena pumilio) from Some Locations in Rivers State, Nigeria

Author(s):  
Douglas, Salome Ibietela ◽  
Chika C. Amadi

Aim:  To determine and compare the bacterial flora and proximate composition of edible frogs from some selected Local Government Areas (LGA) of Rivers State. Place and Duration of Study: Live adult edible frogs were collected from six locations in six Local Government Areas in Rivers State which includes; Biara (E 7º29’ 2.097’’, N 4º78’ 70.608’’) in Gokana, Rumuodumaya (E 7º0’ 57.16.908’’, N 4º52’ 40.398’’) in Obio/Akpo, Umuikere Oyigbo (E 6º55’ 50.606’’, N 9º1’ 13.831’’) in Oyigbo, Diobu (E 6º59’ 6.882’’, N 4º48’ 29.514’’) in Port Harcourt, Igwuruta (E 4º55’ 57.006’’, N 7º1’13.692’’) in Ikwerre and Akpajo (E 5º50’ 67.306’’, N 9º1’ 43.112’’) in Eleme Local Government Areas of Rivers State. This study was conducted from July 2017 to February 2018, covering both the wet and dry seasons. Methodology: The microbiological analyses were carried out using standard microbiological methods. Conventional and molecular identification methods were used to identify the bacteria isolated. Proximate compositions of the two species of edible frogs were also determined using standard methods. Analysis of variance (ANOVA) was used to test for significant difference between the data obtained from the various locations using a computer based program SPSS version 22. Results: Results revealed that Ptychadena pumilios had high bacteria counts across the six Local Government areas. Counts of total heterotrophic bacteria  for Ptychadena pumilios ranged from 1.0 x108cfu/g to 9.0 x108cfu/g, Total Staphylococcus count ranged from 1.0 x105cfu/g to 9.4 x105cfu/g, Total coliform counts ranged 1.0 x105cfu/g to 8.9 x105 cfu/g, Total Feacal Coliform counts ranged from 1.4 x104cfu/g to 9.0 x104cfu/g. while for Ptychadena mascareniensis Total heterotrophic bacterial counts ranged from 2.2 x108 to 4.5 x107cfu/g, Staphylococcal counts ranged from 1.8 x105cfu/g  to 9.5  x105cfu/g, Total coliform counts ranged 1.0 x105cfu/g to 9.8 x105cfu/g, Total Feacal Coliform ranged  from 1.2 x104cfu/g 9.8 x104cfu/g. Statistically there were significant differences in bacterial counts in the six studied LGAs. Statistically there were significant differences in the bacterial counts from the six studied locations. A total of 259 bacterial isolate belonging to the following genera: Escherichia, Staphylococcus, Bacillus, Klebsiella, and Pseudomonas were isolated during this study. The proportion of nutritional composition obtained in this study ranged as follow: carbohydrate from 1.82 to 2.24, crude fibre 2.28 to 3.00, ash 3.99 to 4.89%, lipid 11.90 to 12.32%, protein13.74 to14.00%, moisture 64.45 to 65.74%, for the species of the edible frogs tested. Conclusion: The presence of Escherichia coli identified indicates faecal contamination and indicates the possible presence of potential pathogens. Proper processing and cooking of the frog meat will destroy these organisms, thereby preventing food borne infections. Improper handling and cooking may lead to food borne infections with greater effect observed in immunocompromised patents, the elderly and children. Ptychadena mascareniensis and Ptychadena pumilio have high protein contents therefore, they could be considered as an alternative source of protein despite the bacterial load.

2018 ◽  
Author(s):  
LeBari Barine Gboeloh ◽  
Ike-Ihunwo Chikaire Ndamzi

ABSTRACTSoil transmitted helminthes (STHs) are common public health concern among children in Sub saharan Africa. A study to determine the prevalence and intensity of these parasites among pupils of two primary schools in Nkpor and Mgbodohia communities, Obio/Akpor Local Government Area, Rivers State, Nigeria was conducted. The formo-ether concentration technique was used to concentrate and separate the eggs and cysts from the faeces. Out of 107 pupils (56males and 51 females) investigated, 81 (75.7 %) were positive for at least one gastrointestinal helminth. The parasites identified included Ascaris lumbricoide(58.0%), Hookworms(28.4%), Trichirus trichiura(13.6%) and A.lumbricoide + T. trichiura (6.3%). Ascaris lumbricoide was significantly (P<0.05) higher in prevalence than other parasites. Although more females (54.3%) were infected than males (45.7%), there was no significance(P>0.05) difference in the prevalence in relation to sex. Of the 44 males infected, 24(54.5%), 5 (11.4%), 13(29.5%) and 2(4.5%) haboured A. lumbricoide, T. trichiura, Hookworms and mixed infection (A. lumbricoide + T. trichiura) repectively. Out of the 37 females infected, 21 (56.8%), 3(8.1%), 10(27.0%) and 3(8.1%) haboured A. lumbricoide, T. trichiura, Hookworms and mixed infection (A. lumbricoide + T. trichiura) repectively. There was no significance difference in the prevalence of A. lumbricoide and Hookworms between males (54.5%) and females (56.8%). There was significant difference in prevalence among two major age groups (5-10years-45% and 11-15years-41.9%). Children within the age group of 16-20years had the least infection (9.9%). The intensities of A.lumbricoide, T.trichiura and hookworm were 246.5, 107.5 and 187 Epg respectively. The intensity of A. lumbricoide was significantly difference than other parasites identified.Soil transmited helminthes remain a public health concern among children in the study area. Provision of portable water, toilet facilities, good education on the epidemiology of STHs and regular de-worming will enhance control measures.


2016 ◽  
Vol 7 (1) ◽  
pp. 44
Author(s):  
Abosede Anthonia Olufemi Mumuni ◽  
John Worlu Dike ◽  
Azibaolanari Uzoma-Nwogu

This study investigated the effects of teaching trajectories on students’ understanding of difficult concepts in Biology.Two research questions and two null hypotheses guided the study which was carried out in Obio/Akpor LocalGovernment Area of Rivers State. Two public coeducational schools out of thirteen drawn through purposive samplingtechniques were used. The instrument for data collection, the Students’ Photosynthesis Achievement Test (SPAT) wasvalidated and its reliability coefficient was 0.78. Three intact streams of SS 1 classes in the two schools assigned tobook-ending, guided discussion and jigsaws groups were used. The sample consisted of two hundred and thirteen SS 1Biology students. Mean and standard deviations were used to answer the two research questions while paired t-testsand ANCOVA were used to answer the two hypotheses at 0.05 level of significance. The study revealed that though thethree instructional strategies of book-ending, jig-saws and guided discussion enhanced students’ understanding ofdifficult concept in Biology as shown by the improved achievement scores, the jig-saw strategy was the best. It alsorevealed that there was a significant difference between the achievement of males and females in favour of females inthe three groups. Recommendations made based on the findings of the study included that teachers adopt the jigsawstrategy to enhance students’ understanding of difficult concepts in Biology.


2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Nicoline F. Tanih ◽  
Eunice Sekwadi ◽  
Roland N. Ndip ◽  
Pascal O. Bessong

Pathogenic food-borne bacteria have been associated with severe morbidity and mortality in humans and animals. This study was aimed at determining the prevalence ofStaphylococcus aureus, Salmonellaspp., andEscherichia colipresent in cattle and pigs slaughtered in selected abattoirs in Vhembe District and at determining the susceptibility of the isolates to antibiotics. A total of 176 swab samples (28 cattle and 16 pigs) of the rump, flank, brisket, and neck of the animals were analyzed using standard microbiological methods.E. coliisolates were genotyped to detect pathogenic strains. Of the 176 samples, 104 (67.5%) were positive forE. coliand 50 (32.5%) forS. aureus. There was no statistically significant difference(P>0.05)in the isolation rate from the different animal parts or abattoirs. Overall, 14/104 (13.46%) of theE. coliisolates were pathogenic strains which included enteropathogenicE. coli(EPEC) (bfpA) 1.9%, enterotoxigenicE. coli(ETEC) (LT) 3.8%, and enteroaggregativeE. coli(EAEC) (aaiC) 7.6%.E. coliisolates were resistant (100%) to vancomycin and bacitracin.S. aureus(100%) were resistant to oxacillin and nalidixic acid. The presence of resistant strains of these bacteria in food of animal origin could serve as important vehicles transmitting these bacteria to humans. This finding is of epidemiological significance.


Author(s):  
Lawrence O. Amadi ◽  
Christian C. Nwankwo

This study attempts to evaluate culture-dependent microbial and proximate composition of African salad (Abacha) sold at Nkpolu Oroworukwo (mile 3) market, Port Harcourt, Rivers State, Nigeria. Samples were bought from stationary vendors at market within the months of March, 2019 to March, 2021. Garnished African salad (GAS) and ungarnished African salad (UAS) samples were determined by standard microbiological techniques whereas proximate composition (PC) was analysed by the methods of Association of Official of Analytical Chemists (AOAC) Total aerobic plate counts (TAPCs), total coliform counts (TCCs) and fungal counts (FCs) of GAS were higher 4.5x104CFU/g, 5.3x104CFU/g and 1.1x104CFU/g respectively as against 3.4x104CFU/g, 3.8x104 CFU/g and 0.8x104CFU/g for UAG. Microfloral diversity were more in GAS with species such as Escherichia coli, Bacillus subtilis, Klebsiella sp., Micrococcus sp.. Bacillus sp., Enterobacter sp., and Aspergillus sp., Penicillium sp., Rhizopus sp., Fusarium sp., Mucor sp., Saccharomyces and Candida species than in UAS. Nutrient composition, energy (116.00Kcal/100g) and pH (6.4) values were higher in GAS. In contrast, only the moisture (62.52±0.53%) and carbohydrate (23.21±0.21%) contents were slightly higher in UAS than those in GAS. The study revealed that African salad’ bioburden was slightly above the microbiological standard limits of International Commission on Microbiological Specification for Foods (ICMSF) and (Public Health Laboratory Service (PHLS) for RTE foods for mesophilic aerobic bacteria (MAB)/TAPC but exceeded that for total coliforms. However, the microbes detected are likely aetiologic agents of several food borne diseases (FBDs) in human and animal pathologies. Therefore, adequate surveillance, monitoring and compliance with hygienic practices and environmental sanitary measures during processing, fortification and distribution should be enforced by regulatory agencies and stakeholders for wholesome and palatable RTE foods.


Author(s):  
A. A. Okwelle ◽  
B. E. Asikong ◽  
J. A. Lennox

Background: Majority of the human population in semi-urban and urban areas in Nigeria are heavily reliant on well and stream water as the main source of water supply for drinking and domestic use due to the inadequate provision of potable pipe borne water. These groundwater sources can easily be fecally contaminated and thus, increase the incidence and outbreaks of preventable waterborne diseases. This study was carried out to determine the bacteriological quality of some well and stream waters sources in Ikwerre, Emohua and Etche Local Government Areas of Rivers State. Methodology: Twenty-four sampling sites with 8 from each Local Government Area were randomly selected for this study. A total of 48 samples, 2 from each of the sites were collected aseptically for 12 months. Samples were analysed using membrane filtration technique for total heterotrophic bacterial count and total coliform count. The well and stream water samples were also cultured for E. coli, Salmonella, and Vibrio sp. Results: The results show that Emohua Local Government Area had the highest total heterotrophic bacterial count of 5.2 x 103 cfu/ml. This was followed by Ikwerre LGA with total bacterial count of 4.3 x 102 cfu/ml, while Etche LGA had 3.1 x 102 cfu/ml. The total coliform count ranged from 25 cfu/100ml to 50 cfu/100ml for the three LGA’s, whereas the faecal coliform count was between 12 cfu/100ml and 20 cfu/100ml. The total coliform, E. coli, Salmonella and Vibrio counts from the three local government areas occurred above the WHO stipulated zero count in 100ml of water. Conclusion: The results from this study showed contamination of all the wells studied with faecal coliforms thus, indicating the presence of other enteric pathogens and a potential source for waterborne disease outbreaks. Well water in Ikwerre, Emohua and Etche Local Government Areas of Rivers State are not safe for drinking without additional treatment like disinfection or boiling. Periodic testing and constant monitoring of these water sources should also be done to meet up with the World Health Organization Standards in the provision of safe, clean drinking water. 


Author(s):  
N. P. Akani ◽  
I. O. Hakam ◽  
T. Sampson

The menace of opportunistic infections arising from food contamination can be tackled with knowledge of agents effective against the etiologic agents. A bacteriological examination was carried out on Tympanotonos fuscatus (West African Mud Creeper) sold at different markets in Port Harcourt, Nigeria with interest on Pseudomonas aeruginosa and their antibiogram using standard microbiological methods. Results showed that parboiled samples had the mean total heterotrophic bacterial counts between 26.33±11.02x106 and 85.67±3.79x106 cfu/g, total coliform counts, 6.0±1.73x106 and 13.0±7.21x106 cfu/g and total Pseudomonad counts, 0.00 and 0.67±1.16x102 cfu/g. Roasted samples had total heterotrophic bacterial counts between 40.0±1.73x106 and 45.75±3.86x106 cfu/g, total coliform counts, 10.0±7.211x106 and 13.0±9.539 x 106 cfu/g and total Pseudomonad counts, 6.67±4.619x102 and 20.75±22.824 x102 cfu/g. There was difference (p<0.05) in the total heterotrophic bacteria counts of parboiled samples from the different markets but none in coliform and Pseudomonads counts. No difference (p>0.05) was recorded in counts from the roasted samples. Thirty-two (32) Pseudomonas isolates were obtained from the markets occurring least in the parboiled 6 (18.75%) samples compared to roasted 26 (81.25%) samples. Mile3 market had the highest occurrence 3 isolates (50%) for parboiled samples whereas Mile1 market with 1 isolate (16.67%) was lowest. Results of Antibiogram revealed that Pseudomonas species had varying sensitivity to all the antibiotics tested and was most susceptible to Ofloxacin (90.6%) and most resistant to Nalidixic acid (84.4%). Although the counts obtained are within the upper limits (5x107cfu/gm) recommended by the International Commission on Microbiological Specifications for Foods (ICMSF), there is need to properly cook Tympanotonus fuscatus before consumption to reduce risks of opportunistic pathogens. Infections from Pseudomonas species may be managed with appropriate doses of ofloxacin.


Author(s):  
T. Sampson ◽  
L. K. Giami ◽  
J. A. Okedike

Recreational water bodies are water bodies used for recreational activities such as swimming, surfing, water skiing, water diving and sailing. They include rivers, lakes, beaches, spas and swimming pools. This work was therefore aimed at determining the bacteriological profile of recreational water bodies in Orashi region of Rivers State, Nigeria. Surface water samples were collected from two different sites (Orashi River, Mbiama and Sombreiro River, Ahoada) using standard microbiological methods. Upstream, midstream and downstream samples were collected for a period of three months at monthly interval. Standard plate counts were used for total heterotrophic and coliform bacterial counts using standard microbiological media. The total heterotrophic bacterial count ranged from 4.1X104 to 9.5X104 for Orashi River and 3.0X103 to 4.0X103 for Sombreiro River. A significant statistical difference (p < 0.05) however, existed between total heterotrophic bacterial counts of the samples collected from Orashi River, while no statistical difference (p > 0.05) was observed in the total heterotrophic bacterial counts of samples from Sombreiro River. In the comparative analysis of the samples from the two water bodies, no statistical difference (p > 0.05) was recorded in the total coliform count in Orashi and Sombreiro Rivers. The phenotypic characterization identified the isolates to include Staphylococcus spp., Klebsiella spp., Pseudomonas spp., Bacillus spp., Enterococcus spp. and Micrococcus spp., with Klebsiella pneumoniae as the most occurring (26.1%). Klebsiella pneumoniae and Staphylococcus aureus are known for their pathogenic potentials, hence their presence in these recreational sites are of public health importance. Provision of standard recreational facilities in localities will however reduce the dependency on river sites for recreational activities, and as well prevent recreational associated illnesses.


2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
W. D. Nafarnda ◽  
I. E. Ajayi ◽  
J. C. Shawulu ◽  
M. S. Kawe ◽  
G. K. Omeiza ◽  
...  

Bacteriological characteristics of abattoir effluents (wastewater), abattoir water source, and water bodies receiving abattoir wastewater were investigated in Abuja, Nigeria using the multiple-tube fermentation technique. Source of water to the abattoirs and the usage of water bodies receiving abattoir effluents were determined using questionnaires. Bacterial counts ranged from 4.8 × 106 to 5.8 × 105 /100 mL of total coliform (TC), 8.2 × 104 to 3.2 × 104/100 mL of Fecal coliform (FC), 5.2 × 104 to 2.0 × 104/100 mL of Fecal streptococcus and 1.2 × 104 to 2.0 × 103/100 mL of Escherichia coli for abattoir effluents 6.6 × 105 to 6.0 × 105/100 mL of TC, 6.2 × 104 to 1.8 × 104/100 mL of FC, 1.8 × 104 to 6.0 × 103/100 mL of F. streptococcus, and 4.8 × 103 to 6.6 × 102/100 mL of E. coli for water bodies receiving abattoir effluents 100 m downstream. TC bacteria counts for abattoir effluents exceeded recommended limit for discharge into surface water in Nigeria. No significant difference (P<0.05) was observed between bacterial counts of abattoir effluents and receiving water bodies 100 m downstream: an indication of contamination of receiving water bodies by abattoir effluents and possible public and environmental health hazards.


2018 ◽  
Vol 14 (31) ◽  
pp. 71
Author(s):  
Omeodu M. Doris

The study examined the effect of concept mapping in the teaching of physic in senior secondary school in port Harcourt local government area Rivers state. The purpose of the study was to determine the effect of concept mapping on the teaching of physics. The study also found the difference in the academic achievement of students taught physics with concept mapping and conventional teaching method. Eighty-four SS3 physics students were used for the study. Forty-three 43 comprised of the experimental group and fortyone in the control group. The study adopted pre-test post test quasi experimental design. The instrument used for the study was Physics achievement test (PAT).The instrument contained 25 multiple choice questions, in which each of the questions 2 marks. The instrument was validated by two experts in the department of science education in Rivers State University. Pearson product moment correlation coefficient was used to ascertain the instrument’s level of reliability, which resulted to r value of 0.62. findings of the study shows that students taught Physics with concept mapping significantly better than those taught with conventional method. Also the study found that there was no significant difference between male and female students taught Physics with concept mapping. The study recommended that teachers should imbibe concept mapping method in the teaching of Physics so as to enhance students’ comprehension, identification of relationships that exits between concepts and creativity.


Author(s):  
LeBari Barine Gboeloh

Soil transmitted helminthic infections (STHIs) are common public health concern among children in Sub saharan Africa. A study to determine the prevalence of these infections among pupils in two primary schools in Nkpor and Mgbodohia communities, Obio/Akpor Local Government Area, Rivers State, Nigeria was conducted. The formo-ether concentration technique was used to concentrate and separate the eggs and cysts from the faeces. Out of 107 pupils investigated, 81 (75.7 %) were positive for at least one helminthic infection. Although more females (54.3%) were infected than males (45.7%), there was no significant (P&gt;0.05) difference in the prevalence of Soil transmitted helminthic infections in relation to sex. There was a significant difference (P&gt;0.5) in infection among two major age groups (5-10years-45% and 11-15years-41.9%). Children within the age group of 16-20years had the least infection (9.9%). Out of the 81 children positive for STH, 47 (43.9 %,), 23 (21.5%), 11 (10.3%) and 5(4.7%) had Ascaris lumbricoide, Hookworm, Trichirus trichiura and mixed infection (A.lumbricoide + T. trichiura) respectively. Ascaris lumbricoide (43.9%) was significantly (P&lt;0.05) higher in prevalence than other parasites. Soil transmited helminthic infections are a public health among children concern in the study area. Provision of portable water, toilet facilities and good education on the epidemiology of STHIs in addition to regular de-worming will enhance control measures.


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