scholarly journals Viability and in vivo Hypocholesterolemic Effect of Lactobacillus plantarum 29V in Local Honey

2021 ◽  
pp. 24-33
Author(s):  
Ulrich Landry Kamdem Bemmo ◽  
Chancel Hector Momo Kenfack ◽  
Jean Marcel Bindzi ◽  
Raoul Borkeum Barry ◽  
François Zambou Ngoufack
Keyword(s):  
Lactobacillus Plantarum ◽  
Intestinal Tract ◽  
Control Diet ◽  
Hdl Cholesterol ◽  
Probiotic Strain ◽  
In Vivo Studies ◽  
Human Beings ◽  
Food Matrix ◽  
Gastro Intestinal Tract

The conservation of probiotic products requires low temperatures and suitable equipment that are less available in developing countries. The challenge today is to find a local food matrix that can also carry probiotics (microorganisms with benefits for consumers) in the gastro-intestinal tract. The study mainly focus in the current research was to study the use of honey as a food matrix to carry probiotics in treating the cardiovascular disease, hypercholestreolemia. Thus, this study aimed to assess the viability of Lactobacillus plantarum 29V and its in vivo hypocholesterolemic properties when contained in honey. The strain L. plantarum 29V was added in pasteurized honey and was studied its viability in honey and its impact on the physicochemical parameters of honey.¶ For in vivo studies, 0.5 mL of the pasteurized honey containing approximately 108 CFU/mL of L. plantarum 29V were administered to rats fed on a cholesterol-enriched diet (control diet+ pure cholesterol solution (0.04 g/mL) per day per rat) using a feeding syringe; the treatment lasted 4 weeks. Serum lipids were analyzed during the experiment. The results have shown that the probiotic strain L. plantarum 29V can survive in honey for 28 days without affecting the honey’s qualities. Even present in honey, this strain continues to lower serum total cholesterol, (VLDL +LDL)-cholesterol and triglycerides levels of hypercholesterolemic rats. In addition, HDL-cholesterol levels significantly increased, and the atherosclerosis index was significantly lowered. The present study revealed that honey could be used as a food matrix to carry the probiotic Lactobacillus plantarum 29V strain very well into the gastro-intestinal tract. Hence, a probiotic formulation made of pasteurized honey and L. plantarum 29V would be used to treat or prevent hypercholesterolemia if these effects are confirmed in Human beings.

scholarly journals The Separation and 51Chromium Labeling of Human Lymphocytes With In Vivo Studies of Survival and Migration

Blood ◽  
1971 ◽  
Vol 38 (3) ◽  
pp. 360-371 ◽  
Author(s):  
PETER HERSEY
Keyword(s):  
Human Lymphocytes ◽  
Normal Subjects ◽  
The Body ◽  
In Vivo Studies ◽  
Human Beings ◽  
Lymphatic Leukemia ◽  
Circulating Lymphocytes ◽  
Relative Inability ◽  
And Migration

Abstract This study looks at the application of 51Cr labeling of lymphocytes as a method of obtaining in vivo information about the lymphocyte in human beings. Lymphocytes were separated from whole blood by methods based on isopycnic and rate zonal centrifugation techniques and the conditions for 51Cr uptake by the separated lymphocytes standardized to enable a known amount of radioactivity to be injected into the subjects under study. The uptake of the label into various sites in the body was studied by the means of surface probes linked synchronously to a digital printout device and the survival in the circulation estimated by scintillation counting of blood samples taken at various times after injection of the label. The in vivo studies of survival and migration in 10 normal subjects show an initial rapid clearance of cells from the circulation associated with an uptake of cells into spleen and liver sites, and to a lesser extent, into sites over bone marrow and the abdomen. Survival of the circulating lymphocytes after this period appears to be relatively short, with a half-life of 1.7 days. As the available evidence suggests, this short life may be due to the differential trapping of short-lived lymphocytes in the circulation at the expense of the long-lived lymphocytes. Kinetic interpretations of the data indicate an inverse exponential uptake of cells into the sites studied, and the decline over the organs appears to follow the death rate of the cells in the body as a whole. Comparisons with studies in patients having chronic lymphatic leukemia show a relative inability of leukemic lymphocytes to leave the circulation and enter some sites in the body. These preliminary studies indicate the potential of 51Cr labeling as a useful clinical research tool in the study of lymphocytes in human beings.

Impact of probiotic Lactobacillus plantarum TENSIA in different dairy products on anthropometric and blood biochemical indices of healthy adults

2015 ◽  
Vol 6 (3) ◽  
pp. 233-243 ◽  
Author(s):  
P. Hütt ◽  
E. Songisepp ◽  
M. Rätsep ◽  
R. Mahlapuu ◽  
K. Kilk ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Lactobacillus Plantarum ◽  
Functional Properties ◽  
Dairy Products ◽  
Probiotic Strain ◽  
Healthy Adults ◽  
Food Matrix ◽  
Probiotic Lactobacillus ◽  
Daily Dose ◽  
Baseline Values

The blood pressure-lowering effect of dairy products holds the potential to decrease the risk of cardiovascular disease (CVD). An open question is if the successful expression of functional properties of the probiotic strain depends on host biomarkers and/or food matrix properties. The probiotic Lactobacillus plantarum strain TENSIA® (DSM 21380) is a novel microorganism with antimicrobial and antihypertensive functional properties. The aim of this study was to characterise the functional properties of the probiotic L. plantarum TENSIA and compare its effects on host anthropometric, clinical, and blood biomarkers when consumed with cheese or yoghurt. This study involved two double-blinded randomised placebo-controlled exploratory trials (ISRCTN15061552 and ISRCTN79645828) of healthy adults over a three-week period. The three-week consumption of probiotic L. plantarum TENSIA in a daily dose of 1×1010 cfu in probiotic cheese or a daily dose of 6×109 cfu in yoghurt with different content of carbohydrates, proteins, and lipids did not significantly change the body mass index (BMI), plasma glucose and lipid levels, or inflammatory markers in the blood. Reduced lowered systolic and diastolic blood pressure values were detected, regardless of food matrix or baseline values for blood pressure and BMI. In conclusion, our study showed that three-week consumption of the probiotic L. plantarum TENSIA either in cheese or yoghurt lowered diastolic and systolic blood pressure regardless of food matrix and baseline values of blood pressure and BMI, confirming the impact of the functional properties of the probiotic strain in decreasing CVD risk.

scholarly journals A Review on the Anti-Inflammatory Activity of Pomegranate in the Gastrointestinal Tract

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Elisa Colombo ◽  
Enrico Sangiovanni ◽  
Mario Dell'Agli
Keyword(s):  
Gastrointestinal Tract ◽  
Clinical Studies ◽  
Intestinal Tract ◽  
Biological Activities ◽  
Inflammatory Activity ◽  
Beneficial Effects ◽  
Anti Inflammatory ◽  
Gastro Intestinal Tract ◽  
Inflammatory Effect

Several biological activities of pomegranate have been widely described in the literature, but the anti-inflammatory effect in the gastrointestinal tract has not been reviewed till now. The aim of the present paper is to summarize the evidence for or against the efficacy of pomegranate for coping with inflammatory conditions of the gastro-intestinal tract. The paper has been organized in three parts: (1) the first one is devoted to the modifications of pomegranate active compounds in the gastro-intestinal tract; (2) the second one considering the literature regarding the anti-inflammatory effect of pomegranate at gastric level; (3) the third part considers the anti-inflammatory effect of pomegranate in the gut.In vivostudies performed on the whole fruit or juice, peel, and flowers demonstrate antiulcer effect in a variety of animal models. Ellagic acid was the main responsible for this effect, although other individual ellagitannins could contribute to the biological activity of the mixture. Different preparations of pomegranate, including extracts from peels, flowers, seeds, and juice, show a significant anti-inflammatory activity in the gut. No clinical studies have been found, thus suggesting that future clinical studies are necessary to clarify the beneficial effects of pomegranate in the gastrointestinal tract.

scholarly journals The Effects of Using Chemicals to Remove Slime from African Giant Land Snails Flesh during Processing on Some Nutritional and Biochemical Parameters

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Agbor Esther Etengeneng ◽  
Lamye Glory Moh ◽  
Suffo Kamela Arnaud Landry
Keyword(s):  
Biochemical Parameters ◽  
Relative Weight ◽  
Hdl Cholesterol ◽  
Land Snails ◽  
In Vivo Studies ◽  
Albino Rats ◽  
Protein Ratio ◽  
Crude Lipid ◽  
Hematological Indices

The effects of chemicals commonly used in Cameroon to eliminate slime from the flesh of the African giant land snail, Archachatina marginata, during processing on some nutritional and biochemical parameters were investigated. Groups of snails were processed with these chemicals at three different concentrations. Proximate analysis of all the treated snail groups was carried out, and groups with the highest concentration of each chemical were used to compose diets for experimental rats. Thirty weanling male Wistar albino rats ( 31.25 ± 3.09   g ) aged 21days old were distributed into four groups and fed with 10% protein based diets of A. marginata named D1 (washed with only water), D2 (lime C-treated), D3 (alum C-treated), and D4 (salt C-treated). The crude protein contents of the treated groups reduced significantly when compared with the control (CW), with lime C-treated (LC) having the least here and in crude fiber, but higher (LC, LB, and LA) in dry matter. There was a significant reduction in the crude lipid of alum C-treated (AC) and salt A-treated (SA). In vivo studies showed a general decrease in food consumption, weight gained, efficiency of feed utilization (EFU), true protein digestibility (TD) (except D2), and hematological indices (RBCs (red blood cells), PCV (packed cell volume) of the treated groups (D2, D3, D4) when compared to the control (D1). On the other hand, an increase in the relative weight of the liver (RWL), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and total cholesterol was observed with some of the treated diets; meanwhile, protein efficiency ratio (PER), net protein ratio (NPR), relative weight of the kidneys (RWK), HDL cholesterol, and triglycerides were not affected by these diets. These chemicals should only be used at low concentrations or not at all because of its toxicity at high concentrations.

scholarly journals Changes in the distribution of copper and molybdenum after Mo administration and subsequent additional oral or intraperitoneal Cu administration to rats

1982 ◽  
Vol 48 (2) ◽  
pp. 353-364 ◽  
Author(s):  
H. Nederbragt
Keyword(s):  
Intestinal Tract ◽  
Qualitative Difference ◽  
Inbred Rats ◽  
Liver And Kidney ◽  
A Minor ◽  
Gastro Intestinal Tract ◽  
Minor Extent

1. Male WAG/Cpb inbred rats fed on rations containing 1·5 mg copper/kg (deficient) and 6·0 mg Cu/kg (adequate) were supplemented with molybdenum (500 mg/kg diet). Starting at week 0 rats were killed weekly for up to 6 weeks and the caeruloplasmin activity of plasma, the Cu concentration of plasma, liver and kidney and the Mo concentration of liver and kidney were determined. The experiment was repeated with rats fed on diets of the same composition but given additional Cu for periods of 2 weeks. Cu was given orally by increasing dietary Cu to 6·0 mg/kg and 25·0 mg/kg for Cu-deficient and Cu-adequate rats respectively or intraperitoneally by injecting 75 μg and 250 μg every second day to Cu-deficient and Cu-adequate rats respectively.2. After Mo administration to Cu-deficient rats plasma and kidney Cu and liver and kidney Mo increased but caeruloplasmin activity and liver Cu decreased. In Cu-adequate rats plasma, liver and kidney Cu and liver and kidney Mo increased to much higher levels than in Cu-deficient rats. Caeruloplasmin activity was not affected. Fluctuations in plasma Cu and kidney Mo were correlated closely.3. No qualitative difference between the effect of oral or intraperitoneal Cu administered to Mo-treated Cu-deficient or Cu-adequate rats was found. In Cu-deficient Mo-supplemented rats additional Cu increased plasma Cu, caeruloplasmin activity and liver and kidney Cu and Mo. In Cu-adequate Mo-supplemented rats additional Cu decreased plasma Cu and liver and kidney Mo and increased caeruioplasmin activity and kidney Cu and, to a minor extent, liver Cu.4. In view of the assumption that in rats a Cu, Mo and S containing compound, related to Cu-thiomolybdate, may be formed in vivo the results suggest thai Cu binds to the Mo-S part of the compound; when this compound is formed in the gastro-intestinal tract it can not be absorbed and when it is formed at systemic sites it changes the Cu distribution.

scholarly journals In vivotransfer of an R factor within the lower gastro-intestinal tract of sheep

1977 ◽  
Vol 79 (2) ◽  
pp. 259-268 ◽  
Author(s):  
M. G. Smith
Keyword(s):  
Antibiotic Treatment ◽  
Intestinal Tract ◽  
Host Cells ◽  
E Coli ◽  
Adult Sheep ◽  
Resistance Determinants ◽  
R Factor ◽  
Short Period ◽  
Gastro Intestinal Tract

SUMMARYThe transfer of an R factor from donorE. coliintroduced into the rumen of adult sheep to strains of the coliform microflora resident post rumen in the lower gastro-intestinal tract was found to be greatly increased when the animals were subjected to a short period of starvation (ca. 24–48 h). This also resulted in coliform organisms containing the resistance determinants of the R factor being excreted for much longer periods, sometimes for months afterwards. As no antibiotic treatment was given to the animals during these experiments, possession of the R factor should have conferred no selective advantages on the host cells and other plasmids could possibly be transferred similarlyin vivoin sheep or other ruminants and perhaps also within the gut of monogastric animals.

Procoagulant activities of plasma factor VIIc and factor Xc are positively and independently associated with concentrations of the high-density lipoprotein apolipoprotein, apo A-II

2008 ◽  
Vol 100 (09) ◽  
pp. 391-396 ◽  
Author(s):  
Maria Atta ◽  
David Crook ◽  
Faria Shafique ◽  
Desmond G. Johnston ◽  
Ian F. Godsland
Keyword(s):  
High Density Lipoprotein ◽  
Total Cholesterol ◽  
Multivariable Analysis ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
High Density ◽  
Plasma Lipoproteins ◽  
In Vivo Studies ◽  
Plasma Factor

SummaryThe pro- and antiatherogenic roles of apolipoproteins B andA-I, respectively, are well-established although the importance of apolipoprotein A-II remains unclear. There is extensive evidence for the involvement of plasma lipoproteins in haemostatic function. However, in-vivo studies of relationships between haemostatic variables and apolipoprotein concentrations are very limited. Plasma fibrinogen, factors VIIc and Xc (FVIIc and FXc, respectively), apolipoproteins (apo) A-I, A-II and B, triglycerides, total, low-density and high-density lipoprotein (HDL) cholesterol, and cholesterol in HDL subfractions 2 and 3 were measured in 186 apparently healthy Caucasian men (aged 26–78 years; body mass index 19.9–37.8 kg/m2).Associations between haemostatic, apolipoprotein,lipid and lipoprotein variables were explored in uni- and multivariable analyses. Fibrinogen did not correlate with any of the lipid-related variables. FVIIc and FXc were significant positive univariate correlates of total cholesterol (correlation coefficients 0.26,p<0.001 and 0.19,p<0.05,respectively) triglycerides (0.37, p<0.001 and 0.36, p<0.001), and apoB (0.21, p<0.01 and 0.17, p<0.05) and apoA-II (0.19, p<0.05 and 0.29, p<0.001). HDL2 subfraction cholesterol correlated negatively with FVIIc and FXc (-0.20, p<0.01 and –0.22, p<0.01, respectively). In multivariable analysis, only the associations of FVIIc and FXc with total cholesterol, triglycerides and apoA-II remained statistically significant. In conclusion, total cholesterol and triglycerides were the major independent lipid correlates of FVIIc and FXc. The independent and positive associations of apoA-II with FVIIc and FXc suggest a prothrombotic involvement for this apolipoprotein

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