Diagnostic Value of Serum Leptin Level in Critically Ill Septic Child

Introduction: Diagnosis of sepsis is important to reduce morbidity and mortality. Leptin is an important immunoregulatory hormone that enhances a number of immune responses in sepsis. Objective: to assess serum leptin in diagnosing sepsis in critically ill pediatric patients. Subjects and Methods: This study was carried out on 50 children divided into a case group included 40 critically ill patients initially sepsis free and fulfilling 2 of 4 criteria of SIRS admitted to the Pediatric Intensive Care Unit, children hospital, Tanta university and a control group included 10 apparently healthy children. According to the presence or absence of infection, our patients were classified into SIRS group and sepsis group. For all studied patients; serum leptin, CRP and others indicators of sepsis were measured at admission and 72h later, while were measured one time only for control group. Results: patients who developed sepsis had significantly higher serum leptin levels than those of the control group and SIRS (33.9 ± 20.8 vs. 1.9 ± 0.4, 21.5+ 10.1 respectively, p>0.05). Conclusion: Serum leptin may have a role in early diagnosis of sepsis.

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Proadrenomedullin as a prognostic biomarker for critically-ill septic children

10.21203/rs.2.22810/v1 ◽

2020 ◽

Author(s):

Emad E. Ghobrial ◽

Mervat M Khorshied ◽

Sally B Adly ◽

Miriam Magdy Aziz

Keyword(s):

Critically Ill ◽

Pediatric Intensive Care ◽

Diagnostic Value ◽

Control Group ◽

P Value ◽

Future Studies ◽

Promising Tool ◽

Risk Of Mortality ◽

New Biomarkers ◽

Group Serum

Abstract Background: The use of new biomarkers is a promising tool to assess risk of mortality among septic children. The aim of the study was to confirm prognostic and diagnostic value of pro-ADM as a marker in critically ill septic children. Methods: This study was conducted on 40 patients who were admitted at pediatric intensive care unit (PICU), diagnosed as sepsis in addition to 40 patients enrolled as control group. Serum pro-ADM level was measured for both cases and controls. Results: The level of pro-ADM was significantly higher in cases than controls (p-value <0.001). However, no correlation between level of pro-ADM and outcome (p value 0.720) was found. Conclusion: The pro-ADM level was higher in cases than control, thus proved its diagnostic value but can’t be used as prognostic factor. Future studies are recommended and daily measurements are warranted to study its prognostic value.

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Indicators of the kinin blood system in severe forms of acute respiratory viral infections in children

Kazan medical journal ◽

10.17816/kazmj60779 ◽

1982 ◽

Vol 63 (2) ◽

pp. 51-52

Author(s):

V. A. Anokhin ◽

A. D. Tsaregorodtsev

Keyword(s):

Viral Infection ◽

Viral Infections ◽

Severe Form ◽

Blood System ◽

Control Group ◽

Healthy Children ◽

Respiratory Viral Infection ◽

Respiratory Viral Infections ◽

Acute Respiratory Viral Infection ◽

Apparently Healthy

The aim of this work was to study the parameters of the components of the kinin blood system in children with severe forms of acute respiratory viral infections (ARVI) with neurotoxicosis syndrome. 55 children with ARVI (aged from 1 to 6 months - 14, from 6 months to 1 year - 18, from 1 to 3 years - 11, from 3 to 7 years - 12). 38 patients were admitted in the first three days of illness, 12 - on 4-5 days and 5 - at a later date. 30 children had a severe form of acute respiratory viral infection and 25 - moderate. Adenovirus infection was diagnosed in 14 patients, influenza - in 16, parainfluenza - in 7, MS-viral infection in 5, mixed viral infection - in 13. The control group consisted of 10 apparently healthy children.

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Evaluation of depression and self-esteem in children with monosymptomatic nocturnal enuresis: A controlled trial

Archivio Italiano di Urologia e Andrologia ◽

10.4081/aiua.2014.3.212 ◽

2014 ◽

Vol 86 (3) ◽

pp. 212 ◽

Cited By ~ 7

Author(s):

Orhan Koca ◽

Mehmet Akyüz ◽

Bilal Karaman ◽

Zeynep Yesim Özcan ◽

Metin Öztürk ◽

...

Keyword(s):

Children And Adolescents ◽

Nocturnal Enuresis ◽

Depression Scale ◽

Self Esteem ◽

Control Group ◽

Case Group ◽

Healthy Children ◽

Significant Difference ◽

Monosymptomatic Nocturnal Enuresis ◽

Age Range

Objectives: Nocturnal enuresis (NE) is very common and is one of the most common causes for patients to be admitted to urology, pediatrics, child psychiatry and child surgery departments. We aimed to investigate the effect on depression and self-esteem of this disorder that can cause problems on person's social development and human relations. Material and methods: 90 patients who were admitted to our clinic with complaints of nocturnal enuresis were enrolled. Investigations to rule out organic causes were performed in this group of patients. Out of them 38 children and adolescents (age range 8-18 years) with primary monosymptomatic nocturnal enuresis (PMNE) agreed to participate in the study In the same period 46 healthy children and adolescents with a similar age range without bed wetting complaint were included in the study as a control group. The age of the family, educational and socioeconomic level were questioned and Piers-Harris Children's Self-Concept Scale (PHCSCS) and Children's Depression Inventory (CDI) forms were filled out. Results: Mean age of the cases (18 females or 47.4% and 20 males or 52.6%) was 10.76 ± 3.82 years whereas mean age of controls (26 females or 56.5% and 20 males or 43.5%) was 10.89 ± 3.11 years. Depression scale was significantly higher (p = 0.001) in the case group than in the control group (10.42 ± 4.31 vs 7.09 ± 4.35). In both groups there was no statistically significant difference by age and sex in terms of depression scale (p > 0.05). Conclusion: NE is widely seen as in the community and is a source of stresses either for children and for their families. When patients were admitted to physicians for treatment, a multidisciplinary approach should be offered and the necessary psychological support should be provided jointly by child psychiatrists and psychologists.

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The Significance of Routine Biochemical Markers in Patients With Sepsis

10.21203/rs.3.rs-135063/v1 ◽

2020 ◽

Author(s):

Yan Meng ◽

Yue Wang ◽

Wenbin Qiao ◽

Yumei LIU ◽

Liang Wang ◽

...

Keyword(s):

Retrospective Study ◽

Early Diagnosis ◽

Diagnostic Criteria ◽

Assessment Tool ◽

Diagnostic Value ◽

Sepsis Group ◽

Control Group ◽

Diagnostic Efficiency ◽

Infection Group ◽

D Dimer

Abstract Background: Sepsis is a highly complex and fatal syndrome. It is the main cause of death in the intensive care unit. Early diagnosis is beneficial to reduce the mortality of sepsis and improve the prognosis of patients. Therefore, we look forward to finding cheap and fast diagnostic criteria to quickly assess the patient's condition.Methods: This is a retrospective study. The study enrolled 499 patients in the First Affiliated Hospital of Xinjiang Medical University from January 1, 2018 to June 22, 2020, and 96 healthy cases in the same period. Using the diagnostic criteria of bacterial infection, SIRS criteria and Sepsis-2 consensus criteria, 499 patients and 96 healthy cases were divided into 4 groups: sepsis group (n=300), SIRS group (n=151), infection group (n= 48), the control group (n=96). We collected the results of routine laboratory tests, inflammation indicators and blood culture results of these patients. Results: The sepsis group compared with the control group, MCV, NE, WBC, PLT, HB, D-Dimer, PT, CRP, PCT, IL-6, ALB, TBIL, Cr, LAC, CysC and BNP were statistically significant. D-dimer, CRP and PCT have higher diagnostic efficiency. Compared with the difference between the infection group and the SIRS group, PLT and IL-6 are statistically significant, and have a certain diagnostic value. Sepsis group VS infection group, WBC, IL-6, NE and TBIL showed statistical differences in the comparison. The AUC of NE was 67.6, which was the largest among the three. The specificity (95.8%) was the highest, but the sensitivity (49%) was low.Conclusions: This retrospective study shows that NE, WBC, and D-dimer can help in the early diagnosis of sepsis. D-dimer performs best. WBC and NE may have a differential diagnosis significance between the sepsis group and the infection group. This result can provide a timely and convenient assessment tool for early diagnosis of sepsis.

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The Potential Value of Plasma Receptor Interacting Protein 3 for Neonatal Sepsis Diagnosis

10.21203/rs.3.rs-300543/v1 ◽

2021 ◽

Author(s):

Chuchu Gao ◽

Zongtai Feng ◽

Lixia Wang ◽

Xingxing Zhao ◽

Kai Fu ◽

...

Keyword(s):

Neonatal Sepsis ◽

Diagnostic Value ◽

Sepsis Group ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Interacting Protein ◽

Sepsis Diagnosis ◽

Reactive Protein ◽

Potential Value ◽

Hs Crp

Abstract Background: Neonatal sepsis is a systemic inflammatory response syndrome in neonates. The molecular mechanism of neonatal sepsis remains incompletely clarified. The purpose of this study was to explore the potential value of receptor interacting protein 3 (RIP3) in neonatal sepsis.Methods: 93 neonates with sepsis and 93 neonates without infectious diseases were enrolled in this study from September 2019 to March 2021. Plasma RIP3 was detected by enzyme-linked immunosorbent assay (ELISA) and assessed along with whole blood hypersensitive C-reactive protein (hs-CRP) and platelet count (PLT). Differences of RIP3, hs-CRP and PLT between the two groups were compared. Changes of the three indicators in sepsis were also observed after treatment. The diagnostic value of indicators for neonatal sepsis was evaluated by receiver operating characteristic (ROC) curve.Results: In sepsis group, RIP3 and hs-CRP levels were significantly higher than those in control group (RIP3, p ＜ 0.0001; hs-CRP, p ＜ 0.0001), and PLT level was significantly lower than that in control group (p＜0.0001). After treatment, RIP3 and hs-CRP levels among septic survivors had a significant decrease (p＜0.0001) and PLT level had a significant improvement (p=0.0028). With RIP3＞15464.72 pg/mL, CRP>3.24 mg/L, PLT<205.00×109 /L as the positive criteria, the sensitivity of the three indicators in the diagnosis of neonatal sepsis was 68.8%, 64.5%, 59.1%, and the specificity was 91.4%, 82.8%, 79.6%, respectively. The combination of RIP3, CRP and PLT showed 76.3% sensitivity and 94.6% sensitivity.Conclusions: RIP3 may attribute to the early diagnosis and understanding therapeutic effect of neonatal sepsis. The combined detection of RIP3, CRP and PLT may be more effective than individual ones in the diagnosis of neonatal sepsis.

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Detection of Human Parvovirus B19 Infection in Children with Chronic Haemolytic Anaemia in Benha University Hospitals

10.51429/ejmm30208 ◽

2021 ◽

Vol 30 (2) ◽

pp. 51-58

Author(s):

Amal M. Matta ◽

Elsayed M. Abd-Elghany ◽

Abeer A. Aboelazm ◽

Osama Abo. Zaki, ◽

Doaa Abd. Shaker

Keyword(s):

Hemolytic Anemia ◽

Parvovirus B19 ◽

Control Group ◽

P Value ◽

Healthy Children ◽

Igg Antibodies ◽

University Hospitals ◽

History Of ◽

Chronic Hemolytic Anemia ◽

Apparently Healthy

Background: Due to the tropism of human parvovirus B19 to erythroid progenitor cells, infection in patients with an underlying hemolytic disorder such as thalassemia, hereditary spherocytosis, sickle cell disease and Glucose-6-phosphate dehydrogenase deficiency leads to suppression of erythrocyte formation, referred to as transient aplasia crisis (TAC), which may be life-threatening. Objectives: Detection of parvovirus B19 DNA and its IgG antibodies in the serum of children with chronic hemolytic anemia and in apparently healthy children in Benha University Hospitals. Methodology: The study was conducted on 80 children. Forty of them with chronic hemolytic anemia, they were subdivided into 2 groups, Group (1a) included 20 patients without history of aplastic crisis, Group (Ib) included 20 patients with a history of aplastic crisis and 40 age and sex-matched apparently healthy children representing control (Group II). All patients were subjected to full history taking, clinical examination and laboratory investigations. Parvovirus B19 IgG was measured using anti-parvovirus B19 ELISA kits (SUNRED), and parvovirus B19 DNA was detected by using nestedpolymerase chain reaction. Results: The seroprevalence of parvovirus B19 IgG was significantly higher (P value =0.016) in Group Ia (50%) (10 out of 20) and Group Ib (45%) (9 out of 20) than the control group (Group II) (17.5%) (7 out of 40). There was a significant positive correlation between anti-parvovirus B19 IgG and age of all patients, frequency of blood transfusion. The prevalence of parvovirus B19 DNA was 10% (2 out of 20) in group Ia and 30% (6 out of 20) in group Ib and no viral DNA was detected in the controls (P value=0.001). Although 42.3% (11 out of 26) of children with β thalassemia major had a detectable level of antiparvovirus B19 virus IgG antibodies, only (23.1%) (6 out of 26) of them had B19 DNA. Anti-parvovirus B19 IgG antibodies were detected in 4 children out of 5 children of sickle cell anemia (80%) but the the prevalence of Parvovirus B19 DNA was 20% among them. Conclusion: Measures to keep away from iatrogenic and nosocomial infection transmission should be implemented including screening of donated blood for parvovirus B19 especially blood given to patients with blood disorders. Recommendation: Data from this study support the need for introduction of an approved vaccine that mainly protects children with chronic hemolytic anemia against that infection.

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Bowel Frequency in Critically III Children

The Journal of Pediatric Pharmacology and Therapeutics ◽

10.5863/1551-6776-9.3.187 ◽

2004 ◽

Vol 9 (3) ◽

pp. 187-191

Author(s):

Karen D. Dominguez ◽

Matthew E. Borrego

Keyword(s):

Risk Factors ◽

Critically Ill ◽

Prospective Observational Study ◽

Pediatric Intensive Care ◽

Male Gender ◽

Stool Frequency ◽

Critically Ill Children ◽

Healthy Children ◽

Stepwise Linear Regression ◽

Bowel Movements

BACKGROUND Bowel frequency in healthy children has been determined, but it is not well documented in critically ill children. The objectives of this prospective observational study were to determine if critical illness alters stool frequency in children and to identify risk factors that may increase or decrease stool frequency. METHODS Stool frequency was assessed in children admitted to the pediatric intensive care unit (PICU) over a five month period. The median daily number of bowel movements during admission to the PICU was compared to the patient's estimated number of bowel movements prior to illness. Stepwise linear regression was performed to determine which factors best predicted stool frequency in critically ill children. RESULTS Daily stool frequency was significantly reduced (P < 0.001) during PICU stay (median = 0.5; interquartile: 0, 0.8) compared to preadmission stool frequency estimates (median = 2; interquartile: 1, 2.5). Covariates associated with an increase in stool frequency included male gender and length of stay in the PICU. Conversely, the administration of opioids decreased stool frequency. CONCLUSIONS Bowel frequency was reduced by 75% in children admitted to the PICU. The use of opioids was associated with decreased bowel frequency. Male gender and increased PICU stay was associated with increased bowel frequency.

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Serum Dihydropyrimidinase-Like 3 Concentration in Patients with Gastric Cancer and Its Diagnostic Value

Iranian Journal of Public Health ◽

10.18502/ijph.v50i9.7051 ◽

2021 ◽

Author(s):

Huiqiu Zhong ◽

Xiaojiang Luo

Keyword(s):

Gastric Cancer ◽

Diagnostic Value ◽

Control Group ◽

Case Group ◽

Serum Concentrations ◽

Medical College ◽

Healthy Control ◽

Serum Cea ◽

Gastric Cancer Patients ◽

Group Serum

Background: We aimed to investigate the serum concentration of dihydropyrimidinase-like 3 (DPYSL3) in patients with gastric cancer and its clinical significance. Methods: Seventy four patients with gastric cancer from Wuhan Central Hospital, Tongji Medical College, Huazhong University of Science and Technology, China from October 2018 to April 2019 were selected as the case group. Sixty patients with normal gastric mucosa or mild non-atrophic gastritis were selected as the control group. Serum DPYSL3, CA72-4 and CEA concentrations were measured in both groups. Results: The serum DPYSL3 concentration in the case group was significantly higher than that in the healthy control group (22.04±9.22 vs. 8.36±4.19 μg/L, P<0.001). The serum DPYSL3 concentration in patients with advanced gastric cancer was significantly higher than that in early gastric cancer (27.09±9.12 vs. 13.04±8.22 μg/L, P<0.01); serum DPYSL3 concentration was significantly correlated with tumor size, TNM stage and differentiation (P<0.05). When the cutoff value was 20.98 μg/L, the serum DPYSL3 concentration could differentiate the gastric cancer with ROCAUC 0.882 (95% CI: 0.828-0.937) with sensitivity and specificity of 75% and 94%, respectively. Serum CA72-4 concentration could differentiate the gastric cancer from health controls with ROCAUC 0.812 (95% CI: 0.734-0.834), serum CEA concentration could differentiate gastric cancer with ROCAUC 0.612 (95% CI: 0.534 ~ 0.634). The serum concentrations of DPYSL3, CA72-4 and CEA in gastric cancer patients were increased compared to health controls. Conclusion: Three serological markers have complementary diagnostic value for gastric cancer. Serum DPYSL3 is a new potential molecular marker for gastric cancer.

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Diagnostic Value of the TpTe Interval in Children with Ventricular Arrhythmias

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph182212194 ◽

2021 ◽

Vol 18 (22) ◽

pp. 12194

Author(s):

Joanna Jaromin ◽

Grażyna Markiewicz-Łoskot ◽

Lesław Szydłowski ◽

Agnieszka Kulawik

Keyword(s):

Ventricular Arrhythmia ◽

Ventricular Arrhythmias ◽

Clinical Symptoms ◽

Diagnostic Value ◽

Control Group ◽

Healthy Children ◽

T Wave ◽

Electrical Instability ◽

Qt Intervals ◽

Wave Morphology

Background: The changes in the period of ventricular repolarization, i.e., QT interval, QTp (Q-Tpeak) and TpTe interval (Tpeak–Tend), make it possible to assess the electrical instability of the heart muscle, which may lead to the development of life-threatening ventricular arrhythmia. The aim of the study was to determine and evaluate the use of differences in T-wave morphology and durations of repolarization period parameters (QT, TpTe) in resting ECGs for children with ventricular arrhythmias. Methods: The retrospective analysis was made of the disease histories of 80 examined children with resting ECGs, which were admitted to the Children’s Cardiology Department. The study group consisted of 46 children aged 4 to 18 with ventricular arrhythmias and the control group consisted of 34 healthy children between 4 and 18 years of age, with no arrhythmias. Results: The duration of the TpTe interval was significantly (p < 0.001) longer in the group of children with ventricular arrhythmia with abnormal T-wave (bactrian/bifid, humid/biphasic) compared to the TpTe interval in children with ventricular arrhythmia with the normal repolarization period. The duration of the TpTe (p < 0.001), QTcB (p < 0.001) and QTcF (p < 0.001) intervals were significantly longer in the group of children with ventricular arrhythmias and with abnormal T-wave compared to the values of the TpTe, QTcB, and QTcF intervals of the control group with normal morphology of the repolarization period. Only the duration of the TpTe interval was significantly (p = 0.020) longer in the group of children with ventricular arrhythmia without clinical symptoms. Conclusions: Children with benign ventricular arrhythmias recorded on a standard ECG with prolonged TpTe and QT intervals and abnormal T-wave morphology require systematic and frequent cardiac check up with long term ECG recordings due to the possibility of future more severe ventricular arrhythmias. Further follow-up studies in even larger groups of patients are necessary to confirm the values of these repolarization parameters in clinical practice.

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iTRAQ and PRM-based quantitative proteomics in early recurrent spontaneous abortion: biomarkers discovery

Clinical Proteomics ◽

10.1186/s12014-019-9256-y ◽

2019 ◽

Vol 16 (1) ◽

Cited By ~ 2

Author(s):

Ying Cui ◽

Ling He ◽

Chun-Yan Yang ◽

Qian Ye

Keyword(s):

Spontaneous Abortion ◽

Early Stage ◽

Pregnancy Termination ◽

Diagnostic Value ◽

Differentially Expressed ◽

Recurrent Spontaneous Abortion ◽

Control Group ◽

Case Group ◽

Differentially Expressed Proteins ◽

History Of

Abstract Background Early recurrent spontaneous abortion (ERSA) is a common condition in pregnant women. To prevent ERSA is necessary to look for abortion indicators, such as hormones and proteins, in an early stage. Methods Thirty patients with ERSA were enrolled in the case group. In the control group, we recruited 30 healthy women without a history of miscarriage undergoing voluntary pregnancy termination. The differentially expressed proteins in the serum were identified between the two groups using PRM and iTRAQ. Results Seventy-eight differentially expressed proteins were identified. Using GO functional annotation and KEGG pathway analysis, we detected that the most significant changes occurred in the pathway of Fc gamma R-mediated phagocytosis. Meanwhile, using PRM, we identified three proteins that were closely related to abortion, B4DTF1 (highly similar to PSG1), P11464 (PSG1), and B4DF70 (highly similar to Prdx-2). The levels of B4DTF1 and P11464 were down-regulated, while the level of B4DF70 was up-regulated. Conclusions CD45, PSG1, and Prdx-2, were significantly dysregulated in the samples of ERSA and could become important biomarkers for the prediction and diagnosis of ERSA. Larger‑scale studies are required to confirm the diagnostic value of these biomarkers.

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