scholarly journals Antifungal Activity of Flavonoid Isolated from Lindernia crustacea (L) F. Muell against Traditionally Claimed Dermatophytes

2021 ◽  
pp. 512-521
Author(s):  
Smriti Rekha Chanda Das ◽  
Abdul Baquee Ahmed ◽  
Dibyendu Shil ◽  
Trishna Das ◽  
Faruk Alam ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Spectroscopic Method ◽  
Research Work ◽  
Scientific Basis ◽  
Flash Chromatography ◽  
Diffusion Method ◽  
Fungal Strains ◽  
Isolated Compound ◽  
Anti Fungal ◽  
Benzene Extract

Aim: The present study was aimed to establish antifungal activity of flavonoid isolated from Lindernia crustacea (L) F. Muell. Methods: Potent bioactive constituent from benzene extract was isolated through flash chromatography with solvent Toluene and Acetic acid (4:1). Isolated compound was structurally established by spectroscopic method. Antifungal potential of benzene extract as well as isolated compound was tested by disc diffusion method against two fungal strains Candida albicans (C. albicans) and Trichophyton rubrum (T. rubrum). Results: The isolated compound from benzene extract was found to be flavonoid. Benzene extract as well as flavonoid were shown remarkable zone of inhibition against both fungal strains which indicates strong antifungal activity. Conclusion: The experimental results concluded that Lindernia crustacea (L) F. Muell have significant anti fungal activity due to flavonoid content. It is expected that the results of the present research work would be beneficial in establishing the scientific basis of the traditional claims of medicinal values of Lindernia crustacea.

scholarly journals Anti-fungal potential of tridhara (Tridax procumbens) leaves

2016 ◽  
Vol 1 (3) ◽  
pp. 686-689 ◽  
Author(s):  
Md Faruk Miya ◽  
Zakirul Islam ◽  
Sayeed Shahriyar ◽  
Md Riad Hossain Khan ◽  
Md Salim Reza
Keyword(s):  
Antifungal Activity ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Current Investigation ◽  
Disc Diffusion Method ◽  
Fungal Strains ◽  
Methanolic Extracts ◽  
Tridax Procumbens ◽  
Effective Inhibition ◽  
Anti Fungal

The antifungal activity of methanolic extracts of leaves of Tridax procumbens was studied. Fungal strains Aspergillus niger and Aspergillus ocraceous were selected for the study. Disc diffusion method was applied against the selected fungal strains and compared to the drug ciprofloxacin to observe the antifungal activity of the methanolic extracts of Tridax procumbens leaves. The present study demonstrated that the disks of methanolic extracts of T. procumbens showed effective inhibition against A. niger and A. ocraceous compared to ciprofloxacin. The results of the current investigation suggest that the methanolic extracts of T. procumbens leaves can be used for the treatment of the diseases caused by the tested organism and it has significant scope to develop a novel broad spectrum of antifungal herbal formulations.Asian J. Med. Biol. Res. December 2015, 1(3): 686-689

scholarly journals ANTIFUNGAL ACTIVITY OF RED GALANGAL (Alpinia purpurata K. Schum) ETHANOL EXTRACT AGAINST Malassezia furfur AND Microsporum canis

2020 ◽  
Vol 3 (2) ◽  
pp. 76-81
Author(s):  
Rini Lestari
Keyword(s):  
Antifungal Activity ◽  
Secondary Metabolites ◽  
Ethanol Extract ◽  
Average Concentration ◽  
Diffusion Method ◽  
Microsporum Canis ◽  
Malassezia Furfur ◽  
Agar Diffusion Method ◽  
Alpinia Purpurata ◽  
Anti Fungal

Red galangal (Alpinia purpurata) is a plant that belongs to the Zingiberaceae family. Red galangal rhizome is traditionally used to treat phlegm and ringworm. Red galangal rhizome contains secondary metabolites of essential oils, eugnol, sesquiterpenes, pinen, kaemferida, galanagn and galangol which have anti-fungal properties. This study aims to determine the antifungal activity of red galangal rhizome ethanol extract against Malasezia furfur and Microsporum canis using agar diffusion method. Red galangal extract is made with a concentration variation of 2%, 5% and 10%. The results showed the antifungal activity of red galangal ethanol extract in the form of inhibiting diameters at an average concentration of 2%, 5% and 10% against Malasezia furfur was 16.77 mm, 17.91 mm and 19.72 mm. The average inhibitory diameter of Microsporum canis is 10.58 mm, 13.57 mm and 14.51 mm. Based on the results of this study it can be concluded that the ethanol extract of red galangal has antifungal activity against Malasezia furfur and Microsporum canis.

scholarly journals Isolation and Characterizations of new alkaloid 3-deoxy- 3, 11-epoxy cephalotaxine from Clitoria ternatea

2019 ◽  
Vol 9 (4-A) ◽  
pp. 458-462
Author(s):  
MANIVANNAN - RAJAMANICKAM
Keyword(s):  
Fungal Infections ◽  
Bioactive Compound ◽  
Inflammatory Activity ◽  
Clitoria Ternatea ◽  
E Coli ◽  
Fungal Strains ◽  
Anti Inflammatory ◽  
Isolated Compound ◽  
Anti Fungal ◽  
Dose Levels

The present study describes the isolation and structural elucidation of new alkaloid 3-deoxy-3, 11-epoxy cephalotaxine (1) from the flowers of C. ternatea. It was isolated by careful column chromatographic separation of the crude extract on silica gel 60. The structure was established based on UV, IR, 1HNMR, 13CNMR and GC-MS spectroscopy methods. The antibacterial activity of various bacterial and fungal strains and anti-inflammatory activities of the isolated compound and its crude methanol extract was studied. The highest zone of inhibition (13.0 and 12.0 mm) was shown by 1 at a dose of 200 µg/kg against E. coli and S. aureus strains and (16.0, 12.0 mm) against anti-fungal strains of C. albicans and A. flavus. The results indicated that at both dose levels (100 mg and 200 mg / kg) of isolated compound 1 had significant anti-inflammatory activity from 2nd hour onwards. The bioactive compound isolated from this plant can be employed for antimicrobial activity, also for the treatment of various bacterial and fungal infections and to show pronounced anti-inflammatory effects after three hours of injection. Keywords: Clitoria ternatea, Alkaloid, 3-deoxy-3, 11-epoxy cephalotaxine, Anti-microbial activity, Anti-inflammatory activity 

Design, Synthesis, Docking and Computational Pharmacokinetic Profiling of New Pyrazolinyl Thiazolinone Biheterocycles as Potent Antimicrobial Agents

2020 ◽  
Vol 17 (11) ◽  
pp. 1342-1354
Author(s):  
Vinutha Vittala Salian ◽  
Badiadka Narayana ◽  
Balladka Kunhanna Sarojini ◽  
Sharath Chandra Kodandoor ◽  
Anupam Glorious Lobo
Keyword(s):  
Antifungal Activity ◽  
Active Sites ◽  
Antimicrobial Agents ◽  
Building Blocks ◽  
Diffusion Method ◽  
Dilution Method ◽  
Bacterial Strains ◽  
Design Synthesis ◽  
Reference Drug ◽  
Fungal Strains

Background: Development of potential antimicrobial agents is the main aim in the drug discovery process to overcome the problem of drug resistance. Pyrazolines and thiazolinones are extensively used as building blocks for the synthesis of diverse and medicinally important compounds. Methods: In this present work, a new series of functionalized pyrazolinyl-thiazolinone biheterocycles is designed and synthesized from N-pyrazolinecarbothioamide. Antimicrobial screening is carried out in order to discover their potential towards six bacterial and four fungal strains. The zone of inhibition (ZI in mm) was determined by the disc diffusion method and minimum inhibitory concentration (MIC in μg/mL) by macro dilution method. The druggability of these new entities is done through in silico pharmacokinetic profiling using Maestro 2017-1 interface of Schrӧdinger software. Results and Disscusion: Compounds 4c and 4e with chloro and iodo substituents on Nphenylacetamide ring displayed good inhibitory antibacterial activity against the tested bacterial strains with minimum MIC values when compared to the reference drug tetracycline. Compound 3 with an acetic acid derivative showed high antifungal activity among all the tested derivatives. Compound 3 not only showed antifungal activity but also qualified druggability test with no violation of Lipinski rule of five. Conclusion: The capability of the synthesized pyrazolinyl-thiazolinone derivatives was performed to efficiently inhibit the growth of microorganisms against selected bacterial and fungal strains. Further, these compounds are found to be effectively bound to the active sites of attractive target Escherichia coli FabH.

scholarly journals ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF HEDYOTIS CORYMBOSA, INULA RACEMOSE, HOLOSTEMMA ADAKODIEN MEDICINAL PLANTS

2019 ◽  
pp. 25-29
Author(s):  
AJIT KUMAR MARISETTI ◽  
VENKATARATHANAMMA V.
Keyword(s):  
Antifungal Activity ◽  
Medicinal Plants ◽  
Microbial Activity ◽  
Positive Control ◽  
Diffusion Method ◽  
Antibacterial And Antifungal Activity ◽  
Zone Of Inhibition ◽  
Activity Screening ◽  
Antibacterial And Antifungal ◽  
Anti Fungal

Objective: To evaluate the antibacterial and antifungal activity of three different medicinal plants invitro. Oil extracts of the plants from three different families (Hedyotis corymbosa, Inula racemose, Holostemma adakodien) are used to find the antibacterial and antifungal activity. Methods: Study of anti-microbial activity using agar cup diffusion method, inoculums preparation, Anti-bacterial activity screening (well diffusion method), Anti-fungal activity screening (well diffusion method) are the different methods which are used to find the anti-bacterial and anti-fungal activities of the oil extracts of the above medicinal plants. The activity of these oil extracts and the selected antibiotics are evaluated against four bacterial strains (E. Coli, S. aureus and P. aeruginosa) and three Candida fungal strains (albicans, parapsilosis and tropicalis.). The anti microbial activity of Hedyotis corymbosa, Inula racemose, Holostemma adakodien oil extracts zone of inhibition evaluation was compared with positive control of fungal strain Fluconozole and bacterial strain Norfloxacin, Cefepime and Gatifloxacin. Results: The anti microbial activity of Hedyotis corymbosa, Inula racemose, Holostemma adakodien oil extracts zone of inhibition evaluation was compared with positive control of fungal strain Fluconozole and bacterial strain Norfloxacin, Cefepime and Gatifloxacin. Further studies are needed to explore the novel antibacterial bioactive molecules. Conclusion: The plants Hedyotis corymbosa, Inula racemose and Holostemma adakodien have shown significant anti-bacterial and anti-fungal activity.

scholarly journals 3D Series Metal Complexes Containing Schiff Base Ligand with 2,2'-Bipyridine: Synthesis, Characterization and Assessment of Antifungal Activity

2021 ◽  
pp. 34-45
Author(s):  
B. Kpomah ◽  
U. Ugbune ◽  
T. E. Idu
Keyword(s):  
Antifungal Activity ◽  
Metal Ion ◽  
Synergistic Effects ◽  
Research Work ◽  
Pathogenic Fungi ◽  
Multidrug Resistant ◽  
Ftir Spectra ◽  
Diffusion Method ◽  
Penicillium Species ◽  
Chloride Complexes

The rapid increase in the number of multidrug-resistant of most pathogenic organisms is fast becoming a global concern, thus, the discovery of novel active pharmacological compounds against new targets is a matter of urgency. The incorporation of metal ions into organic ligands has introduced metal-organic drugs framework with synergistic effects for novel applications in the biological system. In this research work, metal(II) chloride complexes of copper, nickel and zinc containing methylphenylketone thiosemicarbazone (MPK-TSC) with 2,2’-bipyridine (bipy) were synthesized; they were further characterized by satisfactory microelemental analysis, Fourier Transform InfraRed (FTIR) spectra as well as electronic spectra study. The complexes are proposed to have the formulae [L1ML2(Cl2)] where M=metal ion, L1=methylphenylketone thiosemicarbazone   L2=2,2’-bipyridine. The complexes are of 1:2 (metal:ligand) stoichiometry and non-electrolytes in solution, the bidentate nature of the two ligands was evident from the FTIR spectra. The compounds were screened for their antifungal activity against four pathogenic fungi: Aspergillus niger, Penicillium Species, Rizopus and Candida albicans using disc diffusion method. The activities of the complexes have been found to be greater than those of the metal salts and the uncoordinated ligands.

scholarly journals Evaluation of Antifungal Activity of Various Plant Latex against Selected Fungal Strains

2021 ◽  
Vol 9 (10) ◽  
pp. 875-883
Author(s):  
Mubashira Begum
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Aspergillus Flavus ◽  
Trichoderma Viride ◽  
Aloe Vera ◽  
Diffusion Method ◽  
Calotropis Procera ◽  
Calotropis Gigantea ◽  
Fungal Strains ◽  
Plumeria Rubra

Abstract: Latex is a natural plant polymer and milky white fluid distributed throughout the plant body like leaves, stems, roots and fruits of all flowering plants and consists of proteins, alkaloids, starches, sugars, oils, tannins, resins and gums that coagulate on-air exposure. It is secreted by specialized plant cells called Laticifers. Plants exude latex in response to physical damage. The present study was carried out to assess the potential antifungal activity of latex of five different plants namely Plumeria rubra, Plumeria alba, Aloe vera, Calotropis procera and Calotropis gigantea against three different pathogenic strains of fungi. All five latexes were found to show good to moderated activity against all the three fungal strains, namely Trichoderma viride, Aspergillus flavus and Aspergillus niger. The latex of each plant was tested in two volumes (i.e. 10µl & 20µl), and it was found that the antifungal activity was volume-dependent, and a significant difference was also observed in the case of different fungal strains. The antifungal activity of plant latexes was evaluated by Agar well diffusion method; results revealed that among all the five latexes, the fresh latex of Plumeria rubra (Vol: 10µl &20µl) showed excellent antifungal activity against Aspergillus niger and Aspergillus flavus, whereas fresh latex of Aloe vera (Vol: 10µl & 20µl) showed excellent antifungal activity against Trichoderma viride. The results of the current research imply that the antifungal activity of latex varies with the species of plants and the fungal strains used. The results therefore demonstrated that, the used five latexes effectively inhibited the growth of three tested fungi. Hence, these plant latexes are natural, ecofriendly and can be used as good candidates for the treatment of various fungal diseases. In further words, they can be used for therapy of antifungal-resistant fungi. Keywords: Antifungal activity, Proteins, Alkaloids, Tannins, Resins, Laticifers, Plumeria rubra, Plumeria alba, Aloe vera, Calotropis procera, Calotropis gigantea, Aspergillus flavus, Aspergillus niger, Trichoderma viride, Latex, Agar well diffusion.

scholarly journals ANTIFUNGAL ACTIVITY OF CYANOTIS AXILLARIS (L.) D. DON EX SWEET AGAINST OPPORTUNISTIC FUNGAL STRAINS

2017 ◽  
Vol 9 (7) ◽  
pp. 140
Author(s):  
Anto Suganya Regis ◽  
Jeya Jothi Gabriel
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
High Concentration ◽  
Disc Diffusion Method ◽  
Antifungal Activities ◽  
Fungal Strains ◽  
Methanol Extracts

Objective: The main focus of the study was to compare and determine the antifungal activities of different solvent extracts of Cyanotis axillaris.Methods: The dried whole plant of C. axillaris was extracted by sequential extraction method using solvents hexane, ethyl acetate and methanol based on their polarity. The antifungal activity of the extracts was tested against 12 opportunistic fungal strains by disc diffusion method. Minimum inhibitory concentration (MIC) was determined using microtiter plate method.Results: The hexane, ethyl acetate and methanol extracts showed significant antifungal activities. The highest antifungal activity was recorded for ethyl acetate extract of C. axillaris. In disc diffusion method at high concentration (5 mg/ml), the ethyl acetate extract exhibited the zone of inhibition>30 mm against C. krusei, mentagrophytes, Scopulariopsis sp. and B. cinerea. In MIC the ethyl acetate extract inhibited the growth of T. mentagrophytes, Scopulariopsis sp., B. cinerea in its low dose (0.031 mg/ml). The hexane, ethyl acetate and methanol extracts of C. axillaris did not show activity against M. gypseum, T. rubrum and E. floccosum.Conclusion: This is the first report for the antifungal efficacy of C. axillaris. The results proved that the extracts of C. axillaris have high potential antifungal principles which could fight against the opportunistic and multidrug resistant fungal strains.

scholarly journals Isolation, Characterization and Antimicrobial Activity of Quercetin-3-O-(2”-α-Methyl-p-Coumaryl)-Rutinoside from Delonix elata Flowers

2020 ◽  
pp. 1-11
Author(s):  
A. Rajeshkanna ◽  
M. M. Senthamilselvi ◽  
D. Prabhakaran
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Research Work ◽  
Ethyl Acetate Fraction ◽  
Salmonella Typhi ◽  
Flavonoid Compound ◽  
Diffusion Method ◽  
Curvularia Lunata ◽  
Bacterial Strains ◽  
Isolated Compound

Aims: To investigate the isolated flavonoid compound, characterization and antimicrobial activity of Quercetin-3-O-(2”-α-methyl-p-Coumaryl)-rutinoside from Delonix elata flowers. Place and Duration of Study: The research work was carried out at Research laboratory, Department of chemistry, Periyar E.V.R College, Trichy-23, between May 2016 to January 2018. Methodology: Extraction and fractionation was carried out from the solvents of ethanol, benzene, petroleum ether, diethyl ether and ethyl acetate. The structure of the isolated compound (Quercetin-3-O-(2”-α-methyl-p-Coumaryl)-rutinoside) was elucidated through their physical and chemical methods. The isolated compound was characterized by using various spectral data such as UV, 1H NMR, 13C NMR, MS. Four bacterial strains Salmonella typhi, Escherichia coli, Enterococcus faecalis, Bacillus cereus and two fungal strains Curvularia lunata and Candida albicans were tested by using disc diffusion method. Conclusion: The present study was concluded that the dry sample of ethyl acetate fraction of Delonix elata flowers was Quercetin-3-O-(2”-α-methyl-p-Coumaryl)-rutinoside and it possesses effective antimicrobial activity against bacteria and fungi.

SYNERGISTIC EFFECT OF NIGELLA OIL AND CLOTRIMAZOLE AS ETHOSOMAL GEL FOR IMPROVED TREATMENT OF FUNGAL INFECTIONS

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (04) ◽  
pp. 45-49
Author(s):  
M Momin ◽  
K. Butte ◽  
A. D’Souza ◽  
Keyword(s):  
Thin Film ◽  
Antifungal Activity ◽  
Fungal Infections ◽  
Research Work ◽  
Entrapment Efficiency ◽  
Synergistic Action ◽  
Physiochemical Parameters ◽  
Anti Fungal ◽  
In Vitro Antifungal Activity

The aim of this research work was to develop an ethosomal gel containing nigella oil encapsulated with clotrimazole (CLT) for improved antifungal activity. The ethosomes were prepared using soya lecithin (SpC) and Nigella oil (NO) as lipids by thin film hydration and they exhibited a particle size of 136±69 nm with an entrapment efficiency of 65.44±1.08 %. CLT ethosomal gel exhibited controlled release of CLT with a steady flux of 7.20mcg/cm2/h across cellophane membrane. The ethosomal gel was stable for 180 days with no significant change in physiochemical parameters. In vitro antifungal activity demonstrated a synergistic action of CLT and NO against the fungal strains compared to plain CLT and NO. This signifies that the combination of NO and CLT in an ethosomal gel has tremendous potential to serve as a synergistic topical anti-fungal preparation than simple ethosomal formulation of CLT.

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