Effect of Lycopene on Histological Alterations of Basement Membrane & Fibrosis in Renal Interstitial Tissue of Celebrex Induced Albino Rats; An Experimental Study

Objective: To assess the histological alterations in basement membrane and fibrosis in renal interstitium of albino rats due to celebrex with enhancement by lycopene. Study Design: Experimental study. Abode of Study: Animal House of Jinnah Postgraduate institute, Karachi, Materials and Methods: COX-2 inhibitor and antioxidant medicines were used in this research work. These medications were orally administered in 40 male albino rats weighing 200-220gm for experimentation. Rats were housed in separate pens at 23ºC. Rats were arranged into 4 groups including control horde and three experimental hordes. The medications were dose up orally by gastric tube daily for one month. At completion of experiment, animals were dissected and tissues were well-preserved for staining. Results: In second horde PAS stained kidney segments showed disrupted basement membrane of distended proximal convoluted tubules & ill-defined brush border and fibrosis in renal interstitium, but 3rd horde had intact basement membrane & well-define brush border at the luminal surface of proximal tubular epithelium and there was mild fibrosis in renal interstitium. Conclusion: This study divulges that lycopene convalesce the disrupted basement membrane and fibrosis in second horde.

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Scanning Electron Microscopy of Human Jejunum in Whipple's Disease

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100079279 ◽

1977 ◽

Vol 35 ◽

pp. 354-355

Author(s):

John H. L. Watson ◽

C. N. Sun

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Basement Membrane ◽

Lamina Propria ◽

Brush Border ◽

Whipple’S Disease ◽

Whipple's Disease ◽

Biopsy Specimens ◽

Brush Borders ◽

Scanning Electron

That the etiology of Whipple's disease could be bacterial was first suggested from electron micrographs in 1960. Evidence for binary fission of the bacteria, their phagocytosis by histiocytes in the lamina propria, their occurrence between and within the cells of the epithelium and on the brush border of the lumen were reported later. Scanning electron microscopy has been applied by us in an attempt to confirm the earlier observations by the new technique and to describe the bacterium further. Both transmission and scanning electron microscopy have been used concurrently to study the same biopsy specimens, and transmission observations have been used to confirm those made by scanning.The locations of the brush borders, the columnar epithelial cells, the basement membrane and the lamina propria beneath it were each easily identified by scanning electron microscopy. The lamina propria was completely filled with the wiener-shaped bacteria, Fig. 1.

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Low-affinity transport of pyroglutamyl-histidine in renal brush-border membrane vesicles

AJP Cell Physiology ◽

10.1152/ajpcell.1989.257.5.c971 ◽

1989 ◽

Vol 257 (5) ◽

pp. C971-C975 ◽

Cited By ~ 3

Author(s):

H. A. Skopicki ◽

K. Fisher ◽

D. Zikos ◽

G. Flouret ◽

D. R. Peterson

Keyword(s):

Brush Border ◽

Brush Border Membrane ◽

Membrane Vesicles ◽

Brush Border Membrane Vesicles ◽

Proximal Tubular Cells ◽

Tubular Cells ◽

Luminal Membrane ◽

Renal Brush Border Membrane ◽

Proximal Tubular ◽

Renal Brush Border

These studies were performed to determine if a low-affinity carrier is present in the luminal membrane of proximal tubular cells for the transport of the dipeptide, pyroglutamyl-histidine (pGlu-His). We have previously described the existence of a specific, high-affinity, low-capacity [transport constant (Kt) = 9.3 X 10(-8) M, Vmax = 6.1 X 10(-12) mol.mg-1.min-1] carrier for pGlu-His in renal brush-border membrane vesicles. In the present study, we sought to demonstrate that multiple carriers exist for the transport of a single dipeptide by determining whether a low-affinity carrier also exists for the uptake of pGlu-His. Transport of pGlu-His into brush-border membrane vesicles was saturable over the concentration range of 10(-5)-10(-3) M, yielding a Kt of 6.3 X 10(-5) M and a Vmax of 2.2 X 10(-10) mol.mg-1.min-1. Uptake was inhibited by the dipeptides glycyl-proline, glycyl-sarcosine, and carnosine but not by the tripeptide pyroglutamyl-histidyl-prolinamide. We conclude that 1) pGlu-His is transported across the luminal membrane of the proximal tubule by multiple carriers and 2) the lower affinity carrier, unlike the higher affinity carrier, is nonspecific with respect to other dipeptides.

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Amelioration of renal cortex histological alterations by aqueous garlic extract in gentamicin induced renal toxicity in albino rats: a histological and immunohistochemical study

Alexandria Journal of Medicine ◽

10.1080/20905068.2020.1871179 ◽

2021 ◽

Vol 57 (1) ◽

pp. 28-37

Author(s):

Hala ZE Mohamed ◽

Merry BK Shenouda

Keyword(s):

Renal Cortex ◽

Renal Toxicity ◽

Immunohistochemical Study ◽

Garlic Extract ◽

Albino Rats ◽

Histological Alterations

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An electron microscope study of kidney basement membrane changes in the mouse by lipoteichoic acid from Streptococcus pyogenes

Canadian Journal of Microbiology ◽

10.1139/m87-124 ◽

1987 ◽

Vol 33 (8) ◽

pp. 709-717 ◽

Cited By ~ 6

Author(s):

Ofra Leon ◽

Charles Panos

Keyword(s):

Basement Membrane ◽

Streptococcus Pyogenes ◽

Membrane Damage ◽

Lipoteichoic Acid ◽

Poststreptococcal Glomerulonephritis ◽

Membrane Morphology ◽

Proximal Tubular ◽

Weight Analysis ◽

Membrane Changes ◽

Almost All

Mice injected repeatedly, intraperitoneally or intravenously, for approximately 1 month with a total of 1.04 mg lipoteichoic acid from a nephritogenic strain of Streptococcus pyogenes lost weight. Analysis by electron microscopy revealed that they also exhibited extensive kidney changes in basement membrane morphology which resembled, in part, those observed in human poststreptococcal glomerulonephritis. For example, the glomerular basement membrane became electron dense and exhibited at least a twofold increase in width sporadically within the same preparation after exposure to lipoteichoic acid. Also, whereas appreciable loss or reduction in epithelial foot processes as a result of fusion was clearly evident, epithelial slits and slit membranes or diaphragms between normal foot processes were not selectively affected. In addition, another mostly thickened, highly coiled or serpentinelike basement membrane with amorphous nodules appeared in these preparations. This type membrane was not observed surrounding the capillary lumina and was the most pronounced abnormality apparent in almost all preparations from mice exposed to lipoteichoic acid. Likewise, the proximal tubular basement membrane became variable in width and increased in electron density in mice given lipoteichoic acid as compared with controls. In addition, this membrane was often punctuated with various morphological protrusions originating from only its thickened areas and which extended away from, and not into, the capillary space. This change was only associated with the basement membrane of the proximal tubular capillaries. All membrane changes persisted but gradually subsided, with normal kidney membrane morphology reappearing on the 4th day following the last injection of lipoteichoic acid. The use of mice and minute amounts of lipoteichoic acid to study membrane damage or change in the fine structure of the glomerular or proximal tubular areas at the immediate presymptomatic, and very early symptomatic, phase of streptococcal glomerulonephritis is noted.

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Cardiovascular Side Effect Remotely Related to NSAIDs: A Comparative Experimental Study on Albino Rats

Journal of Anatomical Society of India ◽

10.1016/s0003-2778(11)80016-x ◽

2011 ◽

Vol 60 (2) ◽

pp. 155-159 ◽

Cited By ~ 1

Author(s):

R Niranjan ◽

R Manik ◽

A.K. Srivastava ◽

G. Palit ◽

S.M. Natu

Keyword(s):

Experimental Study ◽

Side Effect ◽

Albino Rats ◽

Cardiovascular Side Effect

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An experimental study of the effects ofMatricaria chamomillaextract on cutaneous burn wound healing in albino rats

Natural Product Research ◽

10.1080/14786410701591713 ◽

2008 ◽

Vol 22 (5) ◽

pp. 422-427 ◽

Cited By ~ 21

Author(s):

Morteza Jarrahi

Keyword(s):

Experimental Study ◽

Wound Healing ◽

Albino Rats ◽

Burn Wound ◽

Burn Wound Healing

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A STUDY OF THE EFFECT OF THE PHYTOCHEMICAL CONTENT OF P. AMERICANALEAF ON HEMATOLOGICAL SYSTEM OF ALBINO RATS

International Journal of Advanced Research ◽

10.21474/ijar01/12711 ◽

2021 ◽

Vol 9 (04) ◽

pp. 464-467

Author(s):

Ngozi a ◽

◽

N. Omeke ◽

Haruna M. Ndahi ◽

◽

...

Keyword(s):

Adverse Effect ◽

Experimental Study ◽

Aqueous Extract ◽

Leaf Extract ◽

Control Group ◽

Albino Rats ◽

Phytochemical Content ◽

Other Substances ◽

And Control ◽

Experimental Group

Given the medicinal importance of the avocado plant in alternative medicine,the present study aimed to study the effect of the phytochemical content of P. americana leaf extract on the hematological system of albino rats. Albino rats were obtained andclustered into sixgroups,with five rats per groupsimultaneously were assigned experimental and control. The rats in the experimental group were administered with varying doses of the prepared aqueous extract of P. americanawithin the period of the study. The rats in the control group were administered with other substances such as water and feed for the study period.The hematological system of the experimental animal was assessed after the administration of the extract, and it was observed that the aqueous extract of P. americana leaf recorded no significant adverse effect on the hematology system of the experimental study animal.

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Evaluation of analgesic activity of levofloxacin in adult albino rats

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20205544 ◽

2020 ◽

Vol 10 (1) ◽

pp. 85

Author(s):

Prasanna Kumari S. ◽

Latha K. ◽

Meeradevi A.

Keyword(s):

Analgesic Activity ◽

Bacterial Infections ◽

Albino Rats ◽

Tail Flick ◽

Albino Rat ◽

Group Iii ◽

Group I ◽

Analgesic Agents ◽

Active Isomer ◽

Animal House

Background: Levofloxacin is a fluorinated quinolone antimicrobial used in the treatment of various bacterial infections and was active against both gram positive and gram negative bacteria. Levofloxacin is an optically active isomer of ofloxacin. Apart from its antibacterial action, it also exhibit antinociceptive properties. This study was conducted with the aim to evaluate the analgesic activity of Levofloxacin in albino rat in comparison with aspirin.Methods: Thirty adult albino rats weighing 150-250 gm were obtained from central animal house. The animals were divided into five groups of six animals each. Group I served as control received normal feed and water. Group II served as standard received tablet aspirin 100 mg/kg (oral) and Group III, IV, V served as test T1, T2, T3 and received tablet levofloxacin 10 mg/kg, 20 mg/kg and 40 mg/kg (oral), respectively. The analgesic effect of levofloxacin was evaluated using Eddy’s hot plate and tail flick methods and compared with standard analgesic aspirin. The values obtained were expressed as mean±SD. Statistical analysis of difference between groups were carried out using one-way analysis of variance (ANOVA). Probability p<0.05 was taken as the level of statistical significance.Results: Levofloxacin at 40 mg/kg showed statistically (p<0.05) elevation in pain threshold and a higher antinociceptive activity in comparison to control and standard groups.Conclusions: In the present study, levofloxacin has showed promising results as an analgesic when compared to the control and standard groups. It may be a lead compound for identifying newer adjuvant analgesic agents.

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