Toxoplasmosis impact on prematurity and low birth weight

Background Toxoplasma gondii, one of the most common parasites, causes toxoplasmosis, one of the most frequent zoonotic diseases worldwide. T. gondii infects about one-third of the world’s population. T. gondii infection is generally considered a major risk for spontaneous abortion, prematurity and low birth weight in the animal sphere. Less commonly, a toxoplasma serological profile is correlated with the particular data of delivery. Acute T. gondii infection during pregnancy often leads to spontaneous abortion and/or a severe injury of the eyes, brain, and other structures of the foetus. Latent T. gondii infection of pregnant women could lead to less obvious but important changes during pregnancy, including the end product of pregnancy and the timing of labour. This study aimed to contribute to the current knowledge by comparing serological T. gondii profiles of pregnant women with prematurity and low birth weights of newborns. Material and methods A retrospective study design was adopted. The study participants included a cohort of 1733 pregnant women who consecutively gave birth to their children and underwent regular antenatal biochemical screening between the 14th and 16th weeks of pregnancy. Prematurity was defined as the liveborn preterm delivery in gestational age of pregnancy <37 weeks. Low birth weight was defined as weight at birth of ≤2499 grams. The complement-fixation test (CFT) provided serological profiles for toxoplasmosis that expresses the overall levels of toxoplasma immunoglobulins of all classes. Enzyme-linked immunosorbent assay (ELISA) tests for IgG and IgM were used simultaneously. IgM positivity helped to differentiate acute from the latent stage of toxoplasmosis. Birth data, especially the week of delivery and fetal weight, were evaluated accordingly. Results Of the 1733 pregnant women, 25% were diagnosed as latent toxoplasma positive, and 75% as toxoplasma negative. There were 87 premature deliveries versus 1646 timely births. We observed 88 low birth weights and 1645 normal fetal weights. We found a statistically significant association between latent toxoplasmosis and prematurity, χ2(1) = 5.471, p = .019 and between latent toxoplasmosis and low birth weight of newborns, χ2(1) = 7.663, p = .006. There was a 1.707 times higher risk of prematurity for toxoplasma-positive women, while the risk for low birth weight was 1.861 times higher. The strength of both tests of association was mild. We tested the correlation between the levels of CFT titres and week of delivery and weight of newborns. No association was found between the level of latent toxoplasmosis and the week of delivery and fetal weight. Conclusion Latent toxoplasmosis was associated with premature birth rate and lower birth weight of newborns. The odds of premature delivery was 1.7 and low birth weight 1.9 times higher in women with latent toxoplasmosis compared to toxoplasma negative women. Even though the strength of the association in our large sample is relatively mild, the combination of latent toxoplasmosis with other adverse factors could cause serious harm. Whole CFT and specific IgG levels of latent toxoplasmosis are not linked to the severity of prematurity or low birth weight in newborns.

The Relationship between Comfort with Writing and Comfort Working with Numbers in STEM

Informed by writer-identity theory explaining links between emotion and identity, this study explores college STEM students’ feelings of comfort pertaining to math literacy, quantitative literacy, writing in STEM, and writing in general. Survey data from STEM majors (N = 134) was analyzed with Spearman rho tests of association. Results indicated that feelings of comfort working with numbers was significantly associated with comfort writing about numbers (rs = .504, p < .001); comfort writing about numbers was significantly associated with comfort writing in STEM (rs = .265, p = .002); and comfort writing in STEM was significantly associated with comfort writing in general (rs = .558, p < .001). This study suggests links between positive emotional experiences, which are implicated in identity performances, of quantitative writing, disciplinary writing, and writing in general. Future research on emotional experience and writer identity across the curriculum and in the disciplines is called for.

Tests of association based on genomic windows can lead to spurious associations when using genotype panels with heterogeneous SNP densities

Dense single nucleotide polymorphism (SNP) panels are widely used for genome-wide association studies (GWAS). In these panels, SNPs within a genomic segment tend to be highly correlated. Thus, association studies based on testing the significance of single SNPs are not very effective, and genomic-window based tests have been proposed to address this problem. However, when the SNP density on the genotype panel is not homogeneous, genomic-window based tests can lead to the detection of spurious associations by declaring effects of genomic windows that explain a large proportion of genetic variance as significant. We propose two methods to solve this problem.

A case study of a conservation flagship species: the monarch butterfly

What makes a flagship species effective in engaging conservation donors? Large, charismatic mammals are typically selected as ambassadors, but a few studies suggest butterflies—and monarchs in particular—may be even more appealing. To gather more information about people’s responses to monarchs, we conducted an empirical study of member submissions to a successful conservation campaign, the Monarch Story Campaign, conducted by the Environmental Defense Fund (EDF). The set of 691 stories along with their associated demographic and donation data was analyzed in a mixed-methods study using qualitative analysis and tests of association. The results showed that people often described encounters with monarchs in childhood and as adults. They expressed strong, positive emotions, and lauded the monarch’s beauty and other “awe-inspiring” qualities and expressed wonder at their lifecycle (i.e., metamorphosis and migration). They also raised conservation themes of distress at monarch loss, calls for action, and caretaking, such as being “fragile” and “in need.” Sharing personal encounters was associated with current efforts to save the species and more past financial donations, while a second pattern tied more donations to awe at the monarch’s mass migration. These results imply that conservation campaigns built around species people encounter may build lifelong awareness, concern, and actions towards conservation.

A Case Study of a Conservation Flagship Species: The Monarch Butterfly

What makes a flagship species effective in engaging conservation donors? Large, charismatic mammals are typically selected as ambassadors, but a few studies suggest butterflies – and monarchs in particular – may be even more appealing. To gather more information about people’s responses to monarchs, we conducted an empirical study of member submissions to a successful conservation campaign, the Monarch Story Campaign, conducted by the Environmental Defense Fund (EDF). The set of 691 stories along with their associated demographic and donation data was analyzed in a mixed-methods study using qualitative analysis and tests of association. The results showed that people often described encounters with monarchs in childhood and as adults. They expressed strong, positive emotions, and lauded the monarch’s beauty and other “awe-inspiring” qualities and expressed wonder at their lifecycle (i.e., metamorphosis and migration). They also raised conservation themes of distress at monarch loss, calls for action, and caretaking, such as being “fragile” and “in need.” Sharing personal encounters was associated with current efforts to save the species and more financial donations in the past, while a second pattern tied more donations to awe at the monarch’s mass migration. These results imply that conservation campaigns built around species people encounter may build lifelong awareness, concern, and actions towards conservation.

Utility of teleconsultation in accessing eye care in a developing country during COVID-19 pandemic

Objective To evaluate the utility of teleconsultation in the provision of eye care services during the COVID-19 lockdown. Disparities in the consultation burden of sub-specialities and socio-demographic differences in teleconsultation utilization were also assessed. Methods Al-Shifa Trust Eye Hospital Rawalpindi began audio and video teleconsultation using broadband telecommunication services during the lockdown. Patients’ and consultations’ data gathered during the first three weeks after the commencement of this programme were compared with data from the four weeks prior to lockdown. The weekly consultation ratio and overall consultation burden of sub-specialities were measured. Chi-Square tests of association determined the relationship between different variables (socioeconomic status and consultation characteristics) and consultation modality (on-site vs online). Results In total, 17507 on-site consultations (4377/week) were conducted compared to 1431 teleconsultations (477/week), which maintained 10.89% of the weekly pre-lockdown eye care services. The post-lockdown teleconsultation programme saw a relatively higher percentage of service utility among female (47.09% vs 44.71%), younger-age (31.33±19.45 vs 41.25±23.32 years) and higher-socioeconomic-status (32.21% vs 0.30%) patients compared to pre-lockdown on-site consultations. The most common indication for teleconsultation was red-eye (16.70%). While cornea and glaucoma clinics maintained most of the pre-lockdown services (30.42% and 29% respectively), the highest dropout was seen in optometric and vitreoretinal services supporting only 5.54% and 8.28% of pre-lockdown services, respectively. Conclusion Digital initiatives could partially maintain eye care services during the lockdown. Focused strategies to improve teleconsultation utilization are required during the pandemic and beyond.

PheWAS-ME: a web-app for interactive exploration of multimorbidity patterns in PheWAS

Summary Electronic health records (EHRs) linked with a DNA biobank provide unprecedented opportunities for biomedical research in precision medicine. The Phenome-wide association study (PheWAS) is a widely used technique for the evaluation of relationships between genetic variants and a large collection of clinical phenotypes recorded in EHRs. PheWAS analyses are typically presented as static tables and charts of summary statistics obtained from statistical tests of association between a genetic variant and individual phenotypes. Comorbidities are common and typically lead to complex, multivariate gene–disease association signals that are challenging to interpret. Discovering and interrogating multimorbidity patterns and their influence in PheWAS is difficult and time-consuming. We present PheWAS-ME: an interactive dashboard to visualize individual-level genotype and phenotype data side-by-side with PheWAS analysis results, allowing researchers to explore multimorbidity patterns and their associations with a genetic variant of interest. We expect this application to enrich PheWAS analyses by illuminating clinical multimorbidity patterns present in the data. Availability and implementation A demo PheWAS-ME application is publicly available at https://prod.tbilab.org/phewas_me/. Sample datasets are provided for exploration with the option to upload custom PheWAS results and corresponding individual-level data. Online versions of the appendices are available at https://prod.tbilab.org/phewas_me_info/. The source code is available as an R package on GitHub (https://github.com/tbilab/multimorbidity_explorer). Supplementary information Supplementary data are available at Bioinformatics online.

Analysis of pre-prosthetic surgeries performed in a private dental institution

Pre-prosthetic surgery is part of the oral and maxillofacial surgery, which concerns restoration of facial form and oral function. Pre-prosthetic surgery is a surgery done to obtain a better anatomic environment and to provide proper supporting structures for denture construction. The aim of this study was to analyse the various pre-prosthetic surgeries performed in a private dental institution. In this retrospective study, digital case records of all patients who underwent pre-prosthetic surgeries in Saveetha Dental College and Hospital from June 2019 to March 2020 were reviewed. Demographic details of patients and types of pre-prosthetic surgeries performed were recorded from digital case records. Retrieved data was analysed using IBM SPSS Software Version 23.0. Descriptive statistics and tests of association for categorical variables by Chi square tests were done and results were obtained. P value <0.05 was considered statistically significant. In this study, we observed that pre-prosthetic surgery was more commonly performed in the age group of 51-60 yrs (23.3%). Pre-prosthetic surgeries were done more in males than females. The most common type of pre-prosthetic surgery was valvuloplasty (62.2%), followed by frenectomy (20.7%). Statistically, a significant association was found between pre-prosthetic surgery and age group; pre-prosthetic surgery and gender; pre-prosthetic surgery and quadrant involved (p<0.05) Within the limits of this study, it can be concluded that the most common pre-prosthetic surgery performed was valvuloplasty. Pre-prosthetic surgery was performed more in males and the age group of 51-60 years, mostly in the third quadrant.

Inferring the Allelic Series at QTL in Multiparental Populations

Multiparental populations (MPPs) are experimental populations in which the genome of every individual is a mosaic of known founder haplotypes. These populations are useful for detecting quantitative trait loci (QTL) because tests of association can leverage inferred founder haplotype descent. It is difficult, however, to determine how haplotypes at a locus group into distinct functional alleles, termed the allelic series. The allelic series is important because it provides information about the number of causal variants at a QTL and their combined effects. In this study, we introduce a fully Bayesian model selection framework for inferring the allelic series. This framework accounts for sources of uncertainty found in typical MPPs, including the number and composition of functional alleles. Our prior distribution for the allelic series is based on the Chinese restaurant process, a relative of the Dirichlet process, and we leverage its connection to the coalescent to introduce additional prior information about haplotype relatedness via a phylogenetic tree. We evaluate our approach via simulation and apply it to QTL from two MPPs: the Collaborative Cross (CC) and the Drosophila Synthetic Population Resource (DSPR). We find that, although posterior inference of the exact allelic series is often uncertain, we are able to distinguish biallelic QTL from more complex multiallelic cases. Additionally, our allele-based approach improves haplotype effect estimation when the true number of functional alleles is small. Our method, Tree-Based Inference of Multiallelism via Bayesian Regression (TIMBR), provides new insight into the genetic architecture of QTL in MPPs.

Non-use of Contraceptive Methods Among Married: Analysis of the 2018 Guinea Demographic and Health Survey

Background: Modern contraceptive prevalence in Guinea was low in 2018. This study aimed to examine current modern contraceptive non-use among 1086 married women in Guinea who have used a contraceptive method. Methods: We used the dataset of the 2018 Guinea Demographic and Health Survey (GDHS). The analysis included descriptive statistics, tests of association, and logistic regression of women who are not currently using modern contraceptive methods among ever users. Results: Among the sampled women, 454 (42%) did not use modern contraceptive methods. Three out of five non-users made the decision to not use contraceptives. The factors associated with modern contraceptive non-use included living in the administrative regions of Boké (adjusted odds ratio [AOR]: 2.87; 95% confidence interval [CI]: 1.46–5.56) and Mamou (AOR: 3.56; 95% CI: 1.38–9.19); age 25–29 years (AOR: 2.80; 95% CI: 1.25–6.30), 30–34 years (AOR: 3.10; 95% CI: 1.31–7.38), and 45–49 years (AOR: 3.49; 95% CI: 1.22–10.00); and husband’s desire for more children (AOR: 1.58; 95% CI: 1.02–2.46). Conclusions: Interventions that focus on contraceptive non-users among ever users who are currently not using any modern contraceptive method could lead to the resumption of modern contraceptive use among these women. Furthermore, community-level family planning interventions such as male or couple sensitisation or men’s involvement in reproductive issues could improve community perception about contraception and women’s rights for reproductive health.