Chromatin fiber breaks into clutches under tension and crowding

The arrangement of nucleosomes inside chromatin is of extensive interest. While in vitro experiments have revealed the formation of 30 nm fibers, most in vivo studies have failed to confirm their presence in cell nuclei. To reconcile the diverging experimental findings, we characterized chromatin organization using a near atomistic model. The computed force-extension curve matches well with measurements from single-molecule experiments. Notably, we found that a dodeca-nucleosome in the two-helix zigzag conformation breaks into structures with nucleosome clutches and a mix of trimers and tetramers under tension. Such unfolded configurations can also be stabilized through trans interactions with other chromatin chains. Our study supports a hypothesis that disordered, in vivo chromatin configurations arise as folding intermediates from regular fibril structures. We further revealed that chromatin segments with fibril or clutch structures engaged in distinct binding modes and discussed the implications of these inter-chain interactions for a potential sol-gel phase transition.

Development of a new joining technology for hybrid joints of sheet metal and continuous fiber-reinforced thermoplastics

Punctiform mechanical joining technologies, such as riveting, clinching, or screwing, which are widely used in sheet metal processing, are frequently applied because they have been established for many years. Depending on the process, they offer a variety of advantages such as one-sided accessibility, re-detachability, and no need for pre-punching operations or auxiliary joining elements. In addition, the processes often guarantee a high process reliability and extensive process monitoring. However, with thermoplastic composites, they lead to considerable stress concentrations at the joint due to the fibers. Undesirable fiber and inter-fiber breaks then result. With the development of the novel joining technology of joint stamp riveting, an improvement is achieved in this situation that has been described for hybrid joints on components made of thermoplastic composites and metal sheets. The joining principle is based on the formation of a form lock between the joining partners. The thermoplastic composite is thermomechanically formed by means of a joint stamp without using an auxiliary joining element. Within the scope of a research project, the joining process was characterized with regard to the structure of the joining spot, the geometry of the forming tools, and also the mechanical properties for purposes of analyzing and designing the joining process.

SUB-MICROSCALE SPECKLE PATTERN CREATION ON SINGLE CARBON FIBERS FOR IN-SITU DIC EXPERIMENTS

High performance carbon and glass fibers are widely used as reinforcements in composite material systems for aerospace, automotive, and defense applications. Modifications to fiber surface treatment (sizing) is one of the ways to improve the strength of fibers and hence the overall longitudinal tensile strength of the composite. Single fiber tensile tests at the millimeter scale are typically used to characterize the effect of sizing on fiber strength. However, the characteristic length-scale governing the composite failure due to a cluster of fiber breaks is in the micro-scales. To access such micro-scale gage-lengths, we aim to employ indenters of varying radii to transversely load fibers and use scanning electron microscope (SEM) with digital image correlation (DIC) to measure strains at these lengthscales. The use of DIC technique requires creation of a uniform, random, and high contrast speckle pattern on the fiber surface such as that shown in Figure 1. In this work, we investigate the formation of sub-microscale speckle pattern on carbon fiber surface via sputter deposition and pulsed laser deposition techniques (PLD) using Gold-Palladium (Au-Pd) and Niobium-doped SrTiO3 (Nb:STO) targets respectively. Different processing conditions are investigated for both sputter deposition: sputtering current and coating duration, and PLD: number of pulses respectively to create sub-micron scale patterns viable for micro-DIC on both sized and unsized carbon fibers. By varying the deposition conditions and SEM-imaging the deposited patterns on fibers, successful pattern formation at sub-micron scale is demonstrated for both as-received sized and unsized IM7 carbon fibers of average diameter 5.2 μm via sputter deposition and PLD respectively.

MICROSTRUCTURAL CORRELATIONS IN SHORT-ALIGNED GLASS FIBER COMPOSITES

Research shows that in unidirectional composites, fiber breaks and clusters of fiber breaks play a role in the failure onset of the composites [1]. So far, we know no attempts to the date have been made to analyze the fiber break development in short-aligned fiber composites, specifically using E-glass HYBON 2026. O[1]ur challenge is to determine whether there is a similar behavior in aligned short composites and its relationship with the microstructural parameter of fiber orientation distribution. This is important as it is measurable parameter to add value to recycled fiber composites. The more aligned the composite the higher mechanical properties and the higher the added value for recycling. We focused on using synchrotron x-ray computed tomography while performing hold-at-displacement scans to evaluate the fiber microstructure. We found the fiber breaks do not have the same orientation distribution as the non-broken fibers.

A machine learning framework for damage mechanism identification from acoustic emissions in unidirectional SiC/SiC composites

In this work, we demonstrate that damage mechanism identification from acoustic emission (AE) signals generated in minicomposites with elastically similar constituents is possible. AE waveforms were generated by SiC/SiC ceramic matrix minicomposites (CMCs) loaded under uniaxial tension and recorded by four sensors (two models with each model placed at two ends). Signals were encoded with a modified partial power scheme and subsequently partitioned through spectral clustering. Matrix cracking and fiber failure were identified based on the frequency information contained in the AE event they produced, despite the similar constituent elastic properties of the matrix and fiber. Importantly, the resultant identification of AE events closely followed CMC damage chronology, wherein early matrix cracking is later followed by fiber breaks, even though the approach is fully domain-knowledge agnostic. Additionally, the partitions were highly precise across both the model and location of the sensors, and the partitioning was repeatable. The presented approach is promising for CMCs and other composite systems with elastically similar constituents.

CRACKING PHENOMENA OF COATINGS ON LABEL PAPERS

"The phenomenon of paper coating cracking when folded was studied. Coating mixtures were compared according to their composition, applied on different base paper, to find which combinations of the constituents of the base paper and the paper coating results in a lower tendency towards fold cracking of the coating layer. It was shown that the appropriate selection of pigments (calcium carbonate, kaolin) and binders (starch, latex), whose elastic behavior extensively influences the deformation of paper under physical and mechanical stress, is of central importance. The choice of the base paper plays a significant role as well, it should have the longest and the most numerous cellulose fibers possible in order to avoid fiber breaks and inter- and intra-fiber connections. The content of inorganic substances has the greatest negative effect on deteriorating the physical-mechanical properties of the studied label papers, leading to the deposition of inorganic substances at the joints between the cellulose fibers and reducing their bonding."

Fragmentation model for tensile behavior of intermingled hybrid composites and optimal mixing ratio

A numerical fragmentation model is proposed to predict the mechanical response of intermingled, unidirectional hybrid composites under tensile loads. The model is based on a previously developed for unidirectional composites considering the critical number of fiber breaks and the correction of the fiber-matrix interfacial strength. Hybrids comprising two reinforcements are considered, and the energetic contribution of reinforcements is evaluated during the damage process. Additionally, the pseudo-ductile strain, yield strength, and the level of degradation of each reinforcement are estimated. The present model is compared with a progressive failure model and micromechanical finite element simulations, obtaining some similarities in the stress-strain behavior. Results show that both low elongation and high elongation fiber sub-composite experience a linear tensile response where fibers remain intact (IF), and fragmentation (FM) where breaking appears. The sliding/separation phenomenon (SS) occurs in one of the sub-composites when crack saturation is obtained, and failure occurs when the other one undergoes the crack saturation. Results also show that the IF, FM, and SS phenomena are conditioned by the fiber mixing ratio, α. The model allows estimating the optimal value of α for which the highest pseudo-ductile strain and hybrid effect are reached.

Influence of Elongation of Paclitaxel-Eluting Electrospun-Produced Stent Coating on Paclitaxel Release and Transport Through the Arterial Wall after Stenting

It was previously shown that polycaprolactone (PCL)-based electrospun-produced paclitaxel (PTX)-enriched matrices exhibit long-term drug release kinetics and can be used as coatings for drug-eluting stents (DES). The installation of vascular stents involves a twofold increase in stent diameter and, therefore, an elongation of the matrices covering the stents, as well as the arterial wall in a stented area. We studied the influence of matrix elongation on its structure and PTX release using three different electrospun-produced matrices. The data obtained demonstrate that matrix elongation during stent installation does not lead to fiber breaks and does not interfere with the kinetics of PTX release. To study PTX diffusion through the expanded artery wall, stents coated with 5%PCL/10%HSA/3%DMSO/PTX and containing tritium-labeled PTX were installed into the freshly obtained iliac artery of a rabbit. The PTX passing through the artery wall was quantified using a scintillator β-counter. The artery retained the PTX and decreased its release from the coating. The retention of PTX by the arterial wall was more efficient when incubated in blood plasma in comparison with PBS. The retention/accumulation of PTX by the arterial wall provides a prolonged drug release and allows for the reduction in the dose of the drugs in electrospun-produced stent coatings.

A Stochastic Model Based on Fiber Breakage and Matrix Creep for the Stress-Rupture Failure of Unidirectional Continuous Fiber Composites 2. Non-linear Matrix Creep Effects

Stress rupture (sometimes called creep-rupture) is a time-dependent failure mode occurring in unidirectional fiber composites under high tensile loads sustained over long times (e. g., many years), resulting in highly variable lifetimes and where failure has catastrophic consequences. Stress-rupture is of particular concern in such structures as composite overwrapped pressure vessels (COPVs), tension members in infrastructure applications (suspended roofs, post-tensioned bridge cables) and high angular velocity rotors (e.g., flywheels, centrifuges, and propellers). At the micromechanical level, stress rupture begins with the failure of some individual fibers at random flaws, followed by local load-transfer to neighboring intact fibers through shear stresses in the matrix. Over time, the matrix between the fibers creeps in shear, which causes lengthening of local fiber overload zones around previous fiber breaks, resulting in even more fiber breaks, and eventually, formation clusters of fiber breaks of various sizes, one of which eventually grows to a catastrophically unstable size. Most previous models are direct extension of classic stochastic breakdown models for a single fiber, and do not reflect the micromechanical detail, particularly in terms of the creep behavior of the matrix. These models may be adequate for interpreting experimental, composite stress rupture data under a constant load in service; however, they are of highly questionable accuracy under more complex loading profiles, especially ones that initially include a brief “proof test” at a “proof load” of up to 1.5 times the chosen service load. Such models typically predict an improved reliability for proof-test survivors that is higher than the reliability without such a proof test. In our previous work relevant to carbon fiber/epoxy composite structures we showed that damage occurs in the form of a large number of fiber breaks that would not otherwise occur, and in many important circumstances the net effect is reduced reliability over time, if the proof stress is too high. The current paper continues our previous work by revising the model for matrix creep to include non-linear creep whereby power-law creep behavior occurs not only in time but also in shear stress level and with differing exponents. This model, thus, admits two additional parameters, one determining the sensitivity of shear creep rate to shear stress level, and another that acts as a threshold shear stress level reminiscent of a yield stress in the plastic limit, which the model also admits. The new model predicts very similar behavior to that seen in the previous model under linear viscoelastic behavior of the matrix, except that it allows for a threshold shear stress. This threshold allows consideration of behavior under near plastic matrix yielding or even matrix shear failure, the consequence of which is a large increase in the length-scale of load transfer around fiber breaks, and thus, a significant reduction in composite strength and increase in variability. Derivations of length-scales resulting from non-linear matrix creep are provided as Appendices in the Supplementary Material.

Research for the conservation of cultural heritage in the context of the circular economy

The heritage woven objects could be analyzed for defects hidden to the naked eye using non-invasive and non-destructive ultrasonography techniques. Ultrasonography is able to offer information about altered areas, such as gasps, interruptions, discontinuances, narrowed areas, fiber breaks, different densities of the material, defects caused by natural or anthropic factors: improper storage and exposure conditions, the presence of microorganisms and traces of their activity, mechanical causes etc. By recycling of the cotton fibers from other decrepit materials, which are not directly usable, the recondition and rendition of the national and world cultural heritage of these refurbished objects would be accomplished. The impact on the environment is diminished compared to the case when new cotton fibers are created.