Wastewater based environmental surveillance of toxigenic Vibrio cholerae in Pakistan

Background Pakistan has been experiencing intervals of sporadic cases and localized outbreaks in the last two decades. No proper study has been carried out in order to find out the environmental burden of toxigenic V. cholerae as well as how temporal and environmental factors associated in driving cholera across the country. Methods We tested waste water samples from designated national environment surveillance sites in Pakistan with RT-PCR assay. Multistage sampling technique were utilized for samples collection and for effective sample processing Bag-Mediated Filtration system, were employed. Results were analysed by district and month wise to understand the geographic distribution and identify the seasonal pattern of V. cholera detection in Pakistan. Results Between May 2019, and February 2020, we obtained and screened 160 samples in 12 districts across Pakistan. Out of 16 sentinel environmental surveillance sites, 15 sites showed positive results against cholera toxigenic gene with mostly lower CT value (mean, 34±2) and have significant difference (p < 0.05). The highest number of positive samples were collected from Sindh in month of November, then in June it is circulating in different districts of Pakistan including four Provinces respectively. Conclusion V. cholera detection do not follow a clear seasonal pattern. However, the poor sanitation problems or temperature and rainfall may potentially influence the frequency and duration of cholera across the country. Occurrence of toxigenic V. cholerae in the environment samples showed that cholera is endemic, which is an alarming for a potential future cholera outbreaks in the country.

Scyphomedusae and Ctenophora of the Eastern Adriatic: Historical Overview and New Data

One of the obstacles to detecting regional trends in jellyfish populations is the lack of a defined baseline. In the Adriatic Sea, the jellyfish fauna (Scyphozoa and Ctenophora) is poorly studied compared to other taxa. Therefore, our goal was to collect and systematize all available data and provide a baseline for future studies. Here we present phenological data and relative abundances of jellyfish based on 2010–2019 scientific surveys and a “citizen science” sighting program along the eastern Adriatic. Inter-annual variability, seasonality and spatial distribution patterns of Scyphomedusae and Ctenophore species were described and compared with existing historical literature. Mass occurrences with a clear seasonal pattern and related to the geographical location were observed for meroplanktonic Scyphomedusae Aurelia solida, Rhizostoma pulmo, and to a lesser extent Chrysaora hysoscella, Cotylorhiza tuberculata and Discomedusa lobata. Holoplanktonic Pelagia noctiluca also formed large aggregations, which were seasonally less predictable and restricted to the central and southern Adriatic. Four species of Ctenophora produced blooms limited to a few areas: Bolinopsis vitrea, Leucothea multicornis, Cestum veneris and the non-native Mnemiopsis leidyi. However, differences between Adriatic subregions have become less pronounced since 2014. Our results suggest that gelatinous organisms are assuming an increasingly important role in the Adriatic ecosystem, which may alter the balance of the food web and lead to harmful and undesirable effects.

Cruise Vessels Air Pollution Inventory for the Port of Kotor

Prevention of air pollution from ships, according to MARPOL Annex VI, regulates the emission of specific pollutants contained in the exhaust gases into the atmosphere. In this paper, the Port of Kotor is analysed as a case study because of its distinct geographical features, permanent attraction for tourists, and the title of the third busiest port in the Adriatic in 2017. Ships arriving to the Port of Kotor represent one of the major sources of air pollution not only in the Port area, but also in Boka Kotorska Bay as an approach route towards the Port. For calculation of air pollution inventory in this case study, ship engines’ power method was used. The focus of the research analysis is on the cruise ships visiting the Port of Kotor in 2018. For that reason, all ships under 500 GT were excluded from the analysis. The pollution shows clear seasonal pattern characteristic for ports in the Mediterranean. This behaviour is emphasised as an increasing problem that needs to be addressed. The seasonal pattern causes 82.6% of all air pollution from cruise vessels concentrated in the period from May to November.

The seasonal cycle of atmospheric CO2 in the southern hemisphere over the last ten years seen by GOSAT

&lt;p&gt;In the southern hemisphere, the sparse coverage of in-situ CO&lt;sub&gt;2&lt;/sub&gt; measurements prevents a robust determination of regional carbon fluxes and leads to large uncertainties in inverse model results. Therefore, the extensive spatial coverage afforded by satellite CO&lt;sub&gt;2&lt;/sub&gt; measurements is especially valuable there. By analyzing satellite measurements, new insights on the carbon cycle can be derived and carbon cycle models can be validated for the southern hemisphere.&lt;/p&gt;&lt;p&gt;Here, we present a comparison of atmospheric CO&lt;sub&gt;2&lt;/sub&gt; data in Australia provided by the Greenhouse gases Observing SATellite (GOSAT) and the CarbonTracker (CT2019) inverse model from 2009 to 2018. We find that the seasonality of GOSAT CO&lt;sub&gt;2&lt;/sub&gt; is different from that of CarbonTracker across much of the southern hemisphere. This discrepancy follows a clear seasonal pattern with the largest difference of ~2ppm between October and December. We investigate the origin of the discrepancy by utilizing the CO&lt;sub&gt;2&lt;/sub&gt; components provided by CarbonTracker and different fire CO&lt;sub&gt;2&lt;/sub&gt; emission databases. Further, we conduct several sensitivity studies by assimilating GOSAT CO&lt;sub&gt;2&lt;/sub&gt; in the TM5-4DVar data assimilation system, and by transporting different surface fluxes through the TM5 transport model. Our results suggest that the underestimation of local and transported wildfire CO&lt;sub&gt;2&lt;/sub&gt; emissions could cause the observed discrepancy in the seasonality of column CO&lt;sub&gt;2&lt;/sub&gt; between GOSAT and inverse models such as CarbonTracker in the southern hemisphere.&lt;/p&gt;

Alongshore Variability in the Response of a Mixed Sand and Gravel Beach to Bimodal Wave Direction

Characterising spatial and temporal variations in coastal behaviour is essential for the management of beach systems. Recent studies have shown that beach response is more complex in coasts subjected to bimodal wave directions. Despite being pervasive at higher latitudes, relatively little is known about the spatial variability in the response of mixed sand and gravel beaches. This work presents evidence that the response of mixed sand and gravel beaches to bimodal wave directions can be highly variable (both in magnitude and direction of change) even within short shoreline stretches. The analyses focused on beach topography data collected between 2009 and 2018 along five cross-shore transects within a 2-km-long shoreline in Suffolk (East England) and offshore wave data recorded at the West Gabbard Smart buoy. The dominant offshore wave direction oscillates between the southwest and the northeast from year to year, and the bimodal beach sediment has modes at 0.35 mm and 16 mm. Analyses were undertaken considering two timeframes: Biannual surveys from January 2009 to February 2018, and more intensive surveying (from seasonal to pre- and post-storm) from July 2016 to March 2018. Results highlighted large differences in beach response even between transects 350 m apart and no clear seasonal pattern of change. Instead, response seemed to depend on a complex interaction between wave power, dominant wave direction, and local settings. Although correlations were identified between indicators of beach change and wave conditions, these varied across transects. Divergence of longshore transport may occur locally, likely influencing the high alongshore variability.

Another brick in the wall: population dynamics of a symbiotic species of Oxydromus (Annelida, Hesionidae), described as new based on morphometry

Oxydromus humesi is an annelid polychaete living as a strict bivalve endosymbiont (likely parasitic) of Tellina nymphalis in Congolese mangrove swamps and of Scrobicularia plana and Macomopsis pellucida in Iberian saltmarshes. The Congolese and Iberian polychaete populations were previously considered as belonging to the same species, the latter showing regular distribution, intra-specific aggressive behaviour, and complex hostentering behaviour. The fresh Iberian samples enabled us to undertake consistent morphometric analyses, as well as to further analyse the characteristics of the association and the population dynamics of the Iberian population hosted by S. plana. Among the morphological differences between the Congolese and Iberian specimens, leading to the description of the latter as Oxydromus okupa sp. nov., the most important are: 1) longer cephalic appendages, 2) greater distance between the eyes, 3) larger dorsal cirrostyle in relation to the corresponding dorsal lobe and cirrophore. Moreover, dorsal and ventral lobes are similar in length, with the tip of the former reaching the tip of the latter in O. okupa sp. nov., while the dorsal lobe is much shorter than the ventral one in O. humesi. Mature adults of O. okupa sp. nov. occurred during the whole study period, with a higher percentage of ripe females in spring and, particularly, in summer. Numerous host specimens showed the symbiont’s most preferred shell length (>26 - 36 mm). However, the prevalence was very low (usually <5%) and showed a clear seasonal pattern, being lower during spring/summer. This suggests that males are able to leave their hosts during this period, most likely to improve fertilization by directly entering or approaching a host occupied by a ripe female, while females usually remain inside. Based on the new results, the current knowledge of symbiotic Hesionidae and their relationships with invertebrate hosts is updated and discussed.

Vertical and seasonal variations in soil CO2 production in a 55-year-old oriental arborvitae (Platycladus orientalis) plantation in China

The aim of this work was to explore the vertical and seasonal variations of CO2 within the soil profile based on Fick’s law of diffusion in an oriental arborvitae plantation. We continuously measured the soil CO2 concentration profile using CO2 sensors buried at different depths in a coniferous forest in northern China and calculated the CO2 flux based on the profile measurements using a dynamic model. The diurnal pattern of CO2 concentration and flux fluctuated during the day and varied less at night. The CO2 profile had a vertical gradient, with the highest concentrations in the deepest soil layers. The CO2 flux had a clear seasonal pattern with a maximum in summer and a minimum in winter. The contributions of the H, A, B, and C horizons to the total CO2 flux were 75.38, 13.52, 7.61, and 3.49%, respectively. Q10 was 2.668, 4.469, 1.175, and 3.333 in the H, A, B, and C horizons, respectively. The CO2 flux determined from the concentration profiles agreed well with the CO2 flux measured by open dynamic chambers, suggesting that this model could be used to successfully measure soil CO2 emissions and to describe the processes underlying CO2 efflux.

Acoustic telemetry reveals cryptic residency of whale sharks

Although whale sharks ( Rhincodon typus ) have been documented to move thousands of kilometres, they are most frequently observed at a few predictable seasonal aggregation sites. The absence of sharks at the surface during visual surveys has led to the assumption that sharks disperse to places unknown during the long ‘off-seasons’ at most of these locations. Here we compare 2 years of R. typus visual sighting records from Mafia Island in Tanzania to concurrent acoustic telemetry of tagged individuals. Sightings revealed a clear seasonal pattern with a peak between October and February and no sharks observed at other times. By contrast, acoustic telemetry demonstrated year-round residency of R. typus . The sharks use a different habitat in the off-season, swimming deeper and further away from shore, presumably in response to prey distributions. This behavioural change reduces the sharks' visibility, giving the false impression that they have left the area. We demonstrate, for the first time to our knowledge, year-round residency of unprovisioned, individual R. typus at an aggregation site, and highlight the importance of using multiple techniques to study the movement ecology of marine megafauna.

Cyanobacterial occurrence and detection of microcystins and saxitoxins in reservoirs of the Brazilian semi-arid

Aim:The rapid spread of cyanobacteria in water sources and reservoirs has caused serious environmental damage and public health problems, and consists in a problem that challenges the institutions responsible for providing water to the population. In this study, the quantification of microcystin, saxitoxins and cyanobacteria levels was performed over 3 years in the semi-arid reservoirs of Rio Grande do Norte (Brazil). In addition, we analyzed the seasonal distribution of cyanotoxins and the percentage of cyanobacteria and cyanotoxins which were above the limit established by Brazilian law.MethodsThe study was conducted between 2009 and 2011 in four dams with six sites: Armando Ribeiro Gonçalves (ARG) in Itajá, San Rafael (SR) and Jucurutu; Passagem das Traíras (PT); Itans and Gargalheiras (GARG). Cyanobacteria presence were quantified and identified and the presence of microcystins (MCYs) and saxitoxins (STXs) was investigated by ELISA.ResultsThe densities of cyanobacteria were found to be above the permitted in 76% of cases. The ELISA results showed that of the 128 samples analyzed, 27% were above the maximum allowed by the Brazilian Ministry of Health Order 2914/2011. A seasonal pattern for the presence of MCYs was found (0.00227 to 24.1954 µg.L–1), with the highest values in the rainy season. There was no clear seasonal pattern for STXs (0.003 to 0.766 µg.L–1).ConclusionsThis study showed the importance of establishing a water quality monitoring for human consumption and its potability standards since the concentration of MCYs in some samples was above the maximum limit allowed by Brazilian law, thus posing a risk to public health since the conventional water treatment is not able to eliminate these potent hepatotoxins.

Limnological variations of a deep reservoir in periods with distinct rainfall patterns

AIM: This study intends to provide information on how precipitation variation may impact environmental parameters (total phosphorus, water temperature, pH, conductivity, dissolved oxygen, Secchi depth, chlorophyll a and Anabaena abundance) and crustacean zooplankton composition and abundance. METHODS: Samples were collected monthly from October 2000 to September 2002 and from October 2007 to September 2009 at one single sampling station located at the deepest point of the reservoir. All data were obtained in the euphotic zone. RESULTS: The highest values of total phosphorous concentrations were reported together with the maximal values of precipitation. Conversely, conductivity decreased during the wet periods. Anabaena presence was only detected between September and December 2001. Daphnia longispina total abundance showed large inter-annual differences. A clear seasonal pattern was inferred for Diaphanosoma brachyurum and Ceriodaphnia pulchella. Both species are thermophilic and begin to be detected in early summer when the water temperature is around 15 ºC. Copidodiaptomus numidicus abundance did not present a clear seasonal pattern. CONCLUSIONS: Some of the observed shifts in environmental parameters (Conductivity, total phosphorus concentrations and Anabaena abundance) resulting in changes in zooplankton abundance are induced by rainfall variation. The most affected species were those with a lack of seasonality (C. numidicus) or species occurring mainly in winter/early summer (Daphnia longispina). However, a wide range of physical, chemical and biotic interactions is likely to influence zooplankton abundance. Therefore, caution is advised when relating the observed variation exclusively with rainfall intensity. In fact, some of the observed shifts were induced by other factors varying subtly in a seasonal or inter-annual pattern, independently of rainfall intensity.