Universal Nanopore Platform Integrating Multiple Resistive Pulse Sensors into a Single Microfluidic Device

Resistive pulse sensors have been used to characterise everything from whole cells to small molecules. Their integration into microfluidic devices have simplified sample handling whilst increasing throughput. Typically, these devices measure a limited size range or a specific analyte, making them prone to blockages in complex sample matrixes. To prolong their life and facilitate their use, samples are often filtered or prepared to match the sample with the sensor diameter. Here, we advance our tuneable flow resistive pulse sensor which utilises additively manufactured parts. The sensor allows parts to be easily changed, washed and cleaned, its simplicity and versatility allows components from existing nanopore fabrication techniques such as silicon nitride, polyurethane and glass pipettes to be integrated into a single device. This creates a multi-nanopore sensor that can simultaneously measure particles from 0.1 to 30 m in diameter. The orientation and controlled fluid flow in the device allows the sensors to be placed in series, whereby smaller particles can be measured in the presence of larger ones without the risk of being blocked. We demonstrate the device with a range of nanopore materials commonly found within the literature, the easiest to set up was the pulled glass pipette and glass nanopore membrane. However, the glass nanopore membrane was by far the most robust and reusable component tested. We illustrate the concept of a multi-pore flow resistive pulse sensor, by combining an additively manufactured tuneable sensor, termed sensor 1, with a fixed nanopore sensor, termed sensor 2. Sensor 1 measures particles 2 to 30 m in diameter, whilst sensor 2 can be used to characterise particles as small as 100 nm, depending upon its dimensions.

Micro-PIV Measurements of Multiphase Flow in Deforming Porous Media Subject to Mineral Dissolution

Mineral dissolution is studied in novel calcite-based porous micromodels under single- and multiphase conditions, with a focus on the interactions of mineral dissolution with pore flow. Microscopic particle image velocimetry (PIV) was utilized to simultaneously characterize the local velocity field and the instantaneous shapes of the dissolving grains. The preliminary results provide a unique view of the coupled dynamics between pore flow and mineral dissolution.

Adsorption-based membranes for air separation using transition metal oxides

In this work, we use computational modeling to examine the viability of adsorption-based pore-flow membranes for separating gases when a purely size-based separation strategy is ineffective. Using molecular dynamics simulations...

Evidence for the Heterogeneity of the Pore Structure of Rocks from Comparing the Results of Various Techniques for Measuring Hydraulic Properties

We applied three oscillatory methods, the previously presented axial pore-pressure and pore-flow methods, and the laboratory application of the radial oscillatory pore-flow method, and performed steady-state flow-through experiments (Darcy tests), for comparison, in experiments on samples of Westerly granite and Wilkeson sandstone. The granite and the sandstone exhibit pore spaces dominated by micro-fractures and by the granular-medium character with a connected porosity of about 1 and 10 %, respectively. Permeability determined by the axial pore-pressure method shows the closest agreement with the results of the Darcy tests. Apparent porosity and drained modulus derived from specific storage capacity deviate from measured connected porosity and reference values, respectively. The observed deviations of the hydraulic properties between methods suggest that they bear information about the structure of the pore space. Only for the sandstone, anisotropy in hydraulic properties appears to contribute to differences between the results of the various methods. We argue that oscillatory testing provides three indicators for heterogeneity, period dependence, the relation between apparent and connected porosity, and the relation between amplitude ratio and apparent penetration depth, calculated from the simple scaling law for homogeneous materials. These indicators consistently classify the samples of Wilkeson sandstone as hydraulically homogeneous and those of Westerly granite as heterogeneous.

Transport of Turbulence Across Permeable Interface in a Turbulent Channel Flow: Interface-Resolved Direct Numerical Simulation

Turbulence transportation across permeable interfaces is investigated using direct numerical simulation, and the connection between the turbulent surface flow and the pore flow is explored. The porous media domain is constructed with an in-line arranged circular cylinder array. The effects of Reynolds number and porosity are also investigated by comparing cases with two Reynolds numbers ($$Re\approx 3000,6000$$ R e ≈ 3000 , 6000 ) and two porosities ($$\varphi =0.5,0.8$$ φ = 0.5 , 0.8 ). It was found that the change of porosity leads to the variation of flow motions near the interface region, which further affect turbulence transportation below the interface. The turbulent kinetic energy (TKE) budget shows that turbulent diffusion and pressure transportation work as energy sink and source alternatively, which suggests a possible route for turbulence transferring into porous region. Further analysis on the spectral TKE budget reveals the role of modes of different wavelengths. A major finding is that mean convection not only affects the distribution of TKE in spatial space, but also in scale space. The permeability of the wall also have an major impact on the occurrence ratio between blow and suction events as well as their corresponding flow structures, which can be related to the change of the Kármán constant of the mean velocity profile.

Influence of Soil Particle Size on the Temperature Field and Energy Consumption of Injected Steam Soil Disinfection

Soil steam disinfection (SSD) technology is an effective means of eliminating soil borne diseases. Among the soil cultivation conditions of facility agriculture in the Yangtze River Delta region of China, the clay soil particles (SPs) are fine, the soil pores are small, and the texture is relatively viscous. When injection disinfection technology is applied in the clay soil, the diffusion of steam is hindered and the heating efficiency is substantially affected. To increase the heating efficiency of SSD, we first discretized the continuum model of Philip and De Vries into circular particle porous media of different sizes and random distribution. Then with Computational Fluid Dynamics (CFD) numerical simulation technology, a single-injection steam disinfection model for different SP size conditions was constructed. Furthermore, the diffusion pattern of the macro-porous vapor flow and matrix flow and the corresponding temperature field were simulated and analyzed. Finally, a single-pipe injection steam disinfection verification test was performed for different SP sizes. The test results show that for the clay soil in the Yangtze River Delta region of China, the test temperature filed results are consistent with the simulation results when the heat flow reaches H = 20 cm in the vertical direction, the simulation and test result of the heat flow in the maximum horizontal diffusion distance are L = 13 cm and 12 cm, respectively. At the same disinfection time, the simulated soil temperature change trend is consistent with the test results, and the test temperature is lower than the simulated temperature. The difference between the theoretical temperature and the experimental temperature may be attributed to the heat loss in the experimental device. Further, it is necessary to optimize the CFD simulation process and add the disintegration and deformation processes of soil particle size with the change of water content. Furthermore, the soil pores increase as the SP size increases and that a large amount of steam vertically diffuses along the macropores and accumulates on the soil surface, causing ineffective heat loss. Moreover, soil temperature distribution changes from oval (horizontal short radius/vertical long radius = 0.65) to irregular shape. As the SP size decreases, the soil pore flow path becomes fine; the steam primarily diffuses uniformly around the soil in the form of a matrix flow; the diffusion distance in the horizontal direction gradually increases; and the temperature distribution gradually becomes even, which is consistent with the soil temperature field simulation results. Similar to the energy consumption analysis, the maximum energy consumption for SP sizes>27mm and <2mm was 486and 477kJ, respectively. Therefore, proper pore growth was conducive to the diffusion of steam, but excessive pores cause steam to overflow, which increased energy consumption of the system. Considering that the test was carried out in an ideal soil environment, the rotary tiller must be increased for fine rotary tillage in an actual disinfection operation. Although large particles may appear during the rotary tillage process, an appropriate number of large particles contributes to the formation of a large pore flow, under the common effect of matrix flow, it will simultaneously promote greater steam diffusion and heating efficiency. The above theoretical research has practical guiding significance for improving the design and disinfection effect of soil steam sterilizers in the future.

Modern trends in the mathematical simulation of pressure-driven membrane processes

The presented article is an attempt to evaluate the progress in the development of the mathematical simulation of the pressure-driven membrane processes. It was considered more than 170 articles devoted to the simulation of reverse osmosis, nanofiltration, ultrafiltration, and microfiltration and the others published between 2000 and 2010 years. Besides the conventional approaches, which include the irreversible thermodynamics, diffusion and pore flow (and models which consider the membrane surface charge for nanofiltration process), the application of the methods the computational fluid dynamics, artificial neural networks, optimization, and economic analysis have been considered. The main trends in this field have been pointed out, and the areas of using approaches under consideration have been determined. The technological problems which have been solved using the mentioned approaches have also been considered. Although the question of the concentration polarization has not been considered separately, it was defined that, in many cases, the sufficiently accurate model cannot be designed without considering this phenomenon. The findings allow evaluating more thoroughly the development of the simulation of pressure-driven membrane processes. Moreover, the review allows choosing the strategy of the simulation of the considered processes. Keywords: membrane, simulation, model, reverse osmosis nanofiltration, ultrafiltration, microfiltration.

The Calculation of Coal Rock Fracture Conductivity with Different Arrangements of Proppants

An accurate evaluation of coal rock fracture conductivity is an important prerequisite for predicting the productivity of CBM wells. Coal rock is soft and fragile, with low elastic modulus and high Poisson ratio. In the process of fracturing flowback, the contact deformation between proppant and fracture wall will affect the fracture conductivity when the proppant is embedded in the coal rock; thus the calculation method of plate fracture conductivity is no longer suitable for the evaluation of coal rock. Based on the contact deformation theory of elastic mechanics, a method for calculating contact deformation of proppant in fracture is proposed. Considering the effect of the deformation and embedded depth of proppant and the tortuosity of pore flow channel between proppant particles on fracture conductivity, a model for calculating fracture conductivity of coal rock fractures under three kinds of proppant arrangement (Model 4-1, Model 3-1, and Model 2-1) is established. Comparison of calculation results of theoretical model and experiments confirmed that the arrangement of proppant in coal rock fracture is closest to Model 3-1, and the influence of mechanical parameters of coal rock and proppant on fracture conductivity is calculated and analyzed by this theoretical model. The study shows that the coal rock fracture conductivity is affected little by Poisson’s ratio of coal rock and proppant, which is greatly influenced by the elastic modulus of them, and the effect of particle size of proppant is especially significant.