scholarly journals ISOLASI DAN IDENTIFIKASI SENYAWA ANTIOKSIDAN 2-ETILHEKSIL-4-METOKSISINAMAT DARI EKSTRAK BIJI ALPUKAT (Persea americana Mill.)

2021 ◽  
Vol 32 (1) ◽  
pp. 1
Author(s):  
Fauzy Rachman ◽  
Eris Septiana ◽  
Rika Damayanti ◽  
NFN Yadi ◽  
Yatri Hapsari ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Extract ◽  
Persea Americana ◽  
Chemical Laboratory ◽  
Tropical Regions ◽  
Nmr Spectrometry ◽  
Avocado Fruit ◽  
Fourier Transformation Infrared Spectroscopy ◽  
Radical Method ◽  
Avocado Seed

<em><span lang="EN-US">Avocado (</span></em><span lang="EN-US">Persea americana</span><em><span lang="EN-US"> Mill.) is a plant widely found in tropical regions such as Indonesia. Currently, people only consume the avocado flesh and dispose of the seed. This study aimed to examine the antioxidant activity, isolate and identify the active antioxidant structure from avocado seed extracts. The trial was conducted from January to June 2021 at the Chemical Laboratory of Natural Substance, Research Center for Biotechnology, LIPI.  The avocado fruit used in this research was obtained from Cibitung Central Market, Bekasi. The solvents used in the extraction were n-hexane, ethyl acetate, 96% ethanol, and water with multilevel extraction. Antioxidant activity was tested using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical method. The structure of the active compound was determined using UV-Vis spectrophotometry, Fourier Transformation Infrared Spectroscopy (FTIR), and GCMS. The 96% ethanol extract had the highest antioxidant activity with EtOH.4.3.1 isolate as the most active antioxidant isolate with IC50 23,07±1,63 μg.ml-1. The identification results of isolate EtOH.4.3.1 of avocado seed extract with UV-Vis spectrophotometry, FTIR, and GCMS indicated 2-Ethylhexyl-4-methoxycinnamate acting as an antioxidant. Further analysis with Nuclear Magnetic Resonance (NMR) spectrometry of protons and carbon is required to determine the number and position of protons and carbon in the chemical structure.</span></em>

scholarly journals AKTIVITAS ANTIOKSIDAN KECIBELING, BAKAU MERAH, DAN KATUK PADA METODE EKSTRAKSI DAN RASIO EKSTRAK YANG BERBEDA

2020 ◽  
Vol 31 (1) ◽  
pp. 1
Author(s):  
Lilik Sulastri ◽  
Ika Oktavia ◽  
Partomuan Simanjuntak
Keyword(s):  
Antioxidant Activity ◽  
Mesh Size ◽  
Ethanol Extract ◽  
Extraction Methods ◽  
Rhizophora Stylosa ◽  
Red Mangrove ◽  
Water Solvent ◽  
Radical Method ◽  
Infusion Method ◽  
Ethanol Extracts

<em></em><em><em>Indonesian medicinal plants, such as </em>Strobilanthes crispa<em> (L.) Blume (locally known as kecibeling), red mangrove (</em>Rhizophora stylosa<em> Griff.) and star gooseberry {</em>Sauropus androgynus<em> (L.) Merr.} contain active compounds that act as antioxidants. The study aimed to determine the effect of extraction methods (maceration and infusion) on the antioxidant activity of both single extract and  several combination ratios of the extracts mixture of kecibeling leaves, red mangrove leaves and star gooseberry stems. Dried powdered of the samples of a 40 mesh size were extracted using 96 % ethanol solvent (maceration method) and water solvent (infusion method). A single extract or a combination of the three-single extracts (1: 1: 1; 1: 1: 2; 1: 2: 1; and 2: 1: 1) were then evaluated for their antioxidant activity based on the free radical method 2,2-diphenyl-1-picrylhydrazyl (DPPH). In general, extraction method using ethanol (maceration) was better than water (infusion). Antioxidants activities from ethanol extracts of the kecibeling leaves showed the strongest activity with an inhibitory concentration (IC<sub>50</sub>) value of</em> <em>37.65 ppm than the infusion extracts. The combination of a single ethanol extract from the three plants at a ratio of 2: 1: 1 was synergistic, which indicated by its strongest antioxidant activity (IC<sub>50</sub> = 18.78 ppm).  However, it still below the antioxidant activity of vitamin C (IC<sub>50</sub> = 4.24 ppm). Ethanol extract of kecibeling leaves singly or combined with the ethanol extracts of red mangrove leaves and star gooseberry stems can be developed as a potential antioxidant.</em><br /></em>

scholarly journals PENGARUH JENIS PELARUT DAN RASIO BAHAN DENGAN PELARUT TERHADAP KANDUNGAN SENYAWA FENOLIK DAN AKTIVITAS ANTIOKSIDAN EKSTRAK BIJI ALPUKAT (PERSEA AMERICANA MILL.)

2018 ◽  
Vol 7 (2) ◽  
pp. 22
Author(s):  
Ginanjar Rifai ◽  
I Wayan Rai Widarta ◽  
Komang Ayu Nocianitri
Keyword(s):  
Antioxidant Activity ◽  
Experimental Design ◽  
Analysis Of Variance ◽  
Persea Americana ◽  
Total Phenolic ◽  
Experiment Data ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design ◽  
Avocado Seed

This research was conducted in order to determine the type of solvent and ratio between sample and solvent to produce extract avocado seed with the highest antioxidant activity. The experimental design used in this research was a factorial completely randomized design which consisted of two factors. The first factor was the type of solvent consisting of ethanol, methanol, and acetone. The second factor was ratio between sample and solvent of 1:5, 1;10, and 1:15. The treatment was repeated three time to obtain 27 units of the experiment. Data were analyzed with analysis of variance, followed by Duncan test. The results show that the solvent of acetone and ratio between sample and solvent of 1:15 was highest antioxidant activity with total phenolic 803.60 mg/100g, IC50 was 540.95 ppm, and yield 41.36%.

scholarly journals The Bioprocessing Quick Wins from Avocado Fruit in Uganda

2020 ◽  
Vol 5 (5) ◽  
pp. 582-587
Author(s):  
Emmanuel Baidhe ◽  
Nicholas Kiggundu ◽  
Noble Banadda
Keyword(s):  
Industrial Development ◽  
Culture Media ◽  
Biogas Production ◽  
Starch Content ◽  
Persea Americana ◽  
Raw Material ◽  
Biodiesel Production ◽  
Avocado Fruit ◽  
Pharmaceutical Industries ◽  
Avocado Seed

Bioprocessing can help redeem the economic value for avocado (Persea americana) in Uganda. This study reviews the virgin potential of avocado bioprocessing in Uganda. Avocado consists of flesh, seed, and peel. The review indicates that the waste seed and peel are vital for development of high-value products. Both the edible part and waste (peel and seed) can be used for biogas production through a solid-state fermentation process. Biodiesel can be developed using avocado seed oil through a process called transesterification. Avocado oil is a better alternative for biodiesel production compared to waste cooking oil as it requires no further conversions processes for transforming fatty acids to esters. The starch-rich avocado seed is a suitable substrate for bioethanol, pigment and starch production. The high starch content places the avocado fruit as a potential (i) raw material for the production of bioplastics (ii) substrate for bacterial culture media production as opposed to potatoes, cereals, and cassava that double as a staple food. Avocado seeds can also be used for the production of antioxidants relevant for preventing enzymatic browning, thereby increasing product shelf life. Despite the quick wins, there is a need for increased research, financing, personnel training and development of appropriate policies to spur the benefits and untapped potential of avocado bioprocessing in Uganda. The circular economy of avocado waste alone into high-value products could increase gains in the environment and stimulate industrial development especially the cosmetic, food and pharmaceutical industries in Uganda.

scholarly journals KARAKTERISASI DAN AKTIVITAS ANTIOKSIDAN SERAT PANGAN DARI TEPUNG BIJI ALPUKAT (Persea americana Mill)

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Yosep Rudol Parinding ◽  
Edi Suryanto ◽  
Lidya Irma Momuat
Keyword(s):  
Antioxidant Activity ◽  
Fourier Transform ◽  
Dietary Fiber ◽  
Persea Americana ◽  
Soluble Dietary Fiber ◽  
Total Dietary Fiber ◽  
Insoluble Dietary Fiber ◽  
Infra Red ◽  
Phenolic Extract ◽  
Avocado Seed

Penelitian ini bertujuan untuk mengetahui karakteristik dan aktivitas antioksidan serat pangan dari tepung biji alpukat yang diekstraksi menggunakan gelombang ultrasonik dengan pelarut etanol dan aquades. Penelitian ini terdiri dari 3 tahap yaitu mikronisasi, ekstraksi dan karakterisasi. Parameter yang digunakan adalah komposisi proksimat, serat pangan, kandungan hemiselulosa, selulosa, lignin, karakteristik gugus fungsi, aktivitas antioksidan dan kapasitas penangkal nitrit. Hasil karakterisasi secara fisik tepung biji alpukat yang diekstraksi dengan etanol (EBA) dan aquades (ABA) dengan Fourier Transform Infra Red (FTIR) tidak mengalami perubahan mendasar pada komponen utama setelah proses penggilingan dan ekstraksi secara sonikasi. Hasil karakterisasi secara kimia menunjukkan EBA mengandung komposisi kimia seperti air (7,94%), abu (1,87%), protein (5,32%), lemak (0,47%) serat pangan tak larut (18,40%), serat pangan larut (0,24%), serat pangan total (18,64%), hemiselulosa (42,69%), selulosa (4,35%) dan lignin (15,14%). ABA mengandung air (8,84%), abu (1,64%), protein (5,20%), lemak (0,79%) serat pangan tak larut (17,79%), serat pangan larut (0,37%), serat pangan total (18,16%), hemiselulosa (37,71%), selulosa (5,47%) dan lignin (20,08%). Hasil pengujian aktivitas antioksidan menunjukkan bahwa ekstrak fenolik terikat EBA (95,93%) tertinggi diikuti oleh ekstrak fenolik terikat ABA (92,22%), ekstrak fenolik bebas EBA (85,04%) dan ekstrak fenolik bebas EBA (77,51%).ABSTRACTThe objectives of this research were to determine the characteristics and antioxidant activity of dietary fiber from avocado seed powder extracted using ultrasonic waves with ethanol and aquades as solvents. This research consisted of 3 stages, namely micronization, extraction and characterization. The parameters used were proximate composition, dietary fiber, hemicellulose content, cellulose, lignin, functional group characteristics, antioxidant activity and nitrite-scavenging capacity. The results of physical characterization of avocado seed flour extracted with ethanol (EBA) and distilled water (ABA) with Fourier Transform Infra Red (FTIR) did not experience a fundamental change in the main components after the milling and extraction process by sonication. The results of chemical characterization showed that EBA contained chemical compositions such as water (7,94%), ash (1,87%), protein (5,32%), fat (0,47%) insoluble dietary fiber (18,40%), soluble dietary fiber (0,24%), total dietary fiber (18,64%), hemicellulose (42,69%), cellulose (4,35%) and lignin (15,14%). ABA contains water (8,84%), ash (1,64%), protein (5,20%), fat (0,79%) insoluble dietary fiber (17,79%), soluble dietary fiber (0,37%), total dietary fiber (18,16%), hemicellulose (37,71%), cellulose (5,47%) and lignin (20,08%). The results of the antioxidant activity test showed that EBA had the highest bound phenolic extract (95,93%) followed by ABA bound phenolic extract (92,22%), EBA-free phenolic extract (85,04%) and EBA-free phenolic extract (77,51 %).

scholarly journals Uji Aktivitas Antioksidan Ekstrak Etanol Kulit Alpukat (Persea americana Mill.) terhadap Peredaman DPPH

2019 ◽  
Vol 10 ◽  
pp. 72-75
Author(s):  
Amelia Siyanti ◽  
Nurul Fitriani ◽  
Angga
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Chemical Compound ◽  
Extraction Method ◽  
Ethanol Extract ◽  
Positive Control ◽  
Persea Americana ◽  
Dpph Radical ◽  
Inhibition Concentration ◽  
Radical Reduction

Avocado peel is contains chemical compound of alkaloid as antioxidant. So, has been research is to determine the antioxidant activity of ethanol extract avocado peel (Persea americana Mill.) inhibition of DPPH radical (2,2-diphenyl-1-picrylhydrazyl). The extraction method used maceration with 96% ethanol solvent. The ethanol extract avocado peel with variant concentrations are 50 ppm, 75 ppm, 100 ppm, 125 ppm, 150 ppm and ascorbic acid standard as a positive control with variant concentrations are 2 ppm, 4 ppm, 6 ppm, 8 ppm, 10 ppm. Antioxidant activity inhibit DPPH (2,2-diphenyl-1-picrylhydrazyl) radical reduction was measured using Uv-Vis spectrophotometry. The results of antioxidant activity was obtained at IC50 (inhibition concentration) from the ethanol extract avocado is 137.34 ppm and ascorbic acid is 13.18 ppm. The results of ethanol extract avocado peel has moderate antioxidant activity.

Composition of White Potato Starch (Ipomea batatas L.) with Avocado Seed Starch (Persea americana Mill) and Glyserol Concentration in Edible Film

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Musdar Musdar ◽  
Lukmanul Hakim ◽  
Juliani Juliani ◽  
Jailani Jailani
Keyword(s):  
Sweet Potato ◽  
Potato Starch ◽  
Persea Americana ◽  
Edible Film ◽  
Glycerol Concentration ◽  
White Potato ◽  
Ipomea Batatas ◽  
Sweet Potato Starch ◽  
Local Plants ◽  
Avocado Seed

White sweet potato starch (Ipomea batatas L.) and avocado seed starch (Parsea americana Mill) derived from local plants have the potential to be developed as agricultural products. Starch is a hydrocolloid compound as a potential local resource to be utilized. Glycerol function as an anti-freezing which is hygroscopic. This study aims to determine the ratio of white sweet potato starch with avocado seed starch and the concentration of glycerol for making edible film. This study was an experiment using a completely randimized factorial design with 2 (two) main factor consisting of a comparison of white sweet potato starch and avocado seed with 3 levels: P1 = 35%:65%., P2=50%:50%., P3=65%:35% and glycerol concentration with 3 levels: G1=1%., G2=2%., G3=3%. The best result reasearch were content of 23.03% (tratment P1G1), solubility of 55.57% (treatment P3G2)., swelling test of 9.83% (treatment P2g3)., elongation of 8.18% (treatment P3G2)

Antioxidant Activity, Inhibition α-Glucosidase of Ethanol Extract of Strychnos nitida G. Don and Identification of Active Compounds

2019 ◽  
Vol 5 (3) ◽  
pp. 11-20
Author(s):  
Stefani Dhale Rale ◽  
Hasim Hasim ◽  
Syamsul Falah
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Inhibition Activity ◽  
Active Compounds ◽  
Activity Inhibition ◽  
Activity Test ◽  
Ic50 Value ◽  
Treatment Of Diabetes

This study aims to find the treatment of diabetes using natural materials by exploring plants in the province of East Nusa Tenggara. his research was conducted out by extracting the Strychnos nitida G.Don stem using a method of maceration by ethanol 70%. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Simplicia from maceration and fractionation results were then tested for antioxidant activity, α-glucosidase inhibition activity and identification of active compounds. The results showed that ethyl acetate fraction had the lowest IC50 value of 86.83 μg / ml. Results of the α-glucosidase activity test showed that ethyl acetate fraction and n-heksan fraction at 900 ppm had the highest percentage of inhibition of 34.23% and 33.89%. Identification using LCMS/MS method showed that ethyl acetate fraction consist of Benzenemethamine, N, N-dioctyl- as an antioxidantcompound and compound 24-methyl-5-cholestone-hexol as an antidiabetic compound. From the results of this study, we concluded that the extract of kayu ular Strychnos nitida G.Don stem has inhibition activity toward α-glucosidase enzyme.

UJI AKTIVITAS ANTIOKSIDAN FRAKSI N-HEKSAN DAUN AFRIKA (Vernonia amygdalina Del.) DENGAN METODE DPPH

2020 ◽  
Vol 5 (2) ◽  
pp. 250-257
Author(s):  
Nurul Fatimah ◽  
◽  
Reksi Sundu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Vernonia Amygdalina ◽  
Tropical Africa ◽  
Ic50 Value

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

UJI IMUNOSTIMULATOR DARI KOMBINASI EKSTRAK ETANOL HERBA BINARA (ARTEMISIA VULGARIS LINN) DAN EKSTRAK ETANOL DAUN PUCUK MERAH (SYZYGIUM OLEANA) DENGAN METODE HIPERSENSITIVITAS TIPE LAMBAT PADA TIKUS JANTAN

2019 ◽  
Vol 1 (2) ◽  
pp. 22-26
Author(s):  
Romauli Anna Teresia Marbun ◽  
Aminah Syarifuddin ◽  
Montysory Silalahi ◽  
Radika Bella Fista Ginting
Keyword(s):  
Antioxidant Activity ◽  
Immune System ◽  
Secondary Metabolites ◽  
Ethanol Extract ◽  
Positive Control ◽  
Artemisia Vulgaris ◽  
Chemical Screening ◽  
Chemical Content ◽  
High Antioxidant Activity ◽  
New Treatments

Diseases mediated by the immune system are difficult problems to treat such as human immunodeficiency virus (HIV) and other lethal viruses. Infections that occur in normal people are generally brief and rarely leave permanent damage. Treatment of this disease requires an aggressive and innovative approach to the development of new treatments so that it requires the role of immunomodulators to improve the immune system. A substance that acts as an enhancer or immune enhancer can be obtained by using herbs that are efficacious as immunostimulants. One of the herbs used is herbal binara (Artemisia vulgaris L) which has been studied as a potential immunomodulator with high antioxidant activity. Previous research also stated that red shoots (Syzygium oleana) were studied as potential immunomodulators with high antioxidant activity. Several other species such as Syzygium samarangense have 16 flavonoida compounds which show pharmacological immunological activity. The purpose of this study was to determine the content of secondary metabolites of ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) and to determine the best dose of extract from the ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) can reduce the volume of swelling of mouse feet. Examination of the chemical content of secondary metabolites from the ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) is carried out by chemical screening and characterization of simplicia and extract. The method used is the slow type hypersensitivity method. In this test the independent variable is the secondary metabolite of ethanol extract of herb binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) with four concentrations (50, 100, 200 and 400 mg / kgBB). The positive control used by Stimuno dose is 32.5 mg / kgBB

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