Strategic directions of increase of bean production in Ukraine

Goal. Based on the results to substantiate the need for a significant increase in legume crops in Ukraine on the basis of analysis of food and feed indicators of their seeds, the impact on soil quality and the environment. Material and methods. Field – determination of features of growth and development of plants, elements of seed productivity; biometric and measuring-weight – accounting of yield and total biomass; biochemical – determination of protein, fat and other components of seeds; statistical – variation, regression and analysis of variance. Results and discussion. The significant nutritional value of legume seeds, which is rich in high-quality protein with a high content of essential amino acids, isoflavones, essential micronutrients, is substantiated. It is distinguished by high taste, quickly swells and boils, has a pleasant aroma. This group of crops is able to fix nitrogen from the air, provide for their own needs and leave a significant amount of it in the soil for subsequent crop rotations. Legumes should be considered in the crop rotation system together with winter wheat as fallow crops. Their synergistic effect on subsequent crop rotations is explained by the peculiarity of the microflora of the root zone, where symbiotic and free-living bacteria are concentrated. Observations indicate the need to increase crops of pea, chickpea and lentil in Ukraine, as there are all the necessary conditions - adapted to insufficient moisture varieties, developed technology for their cultivation, accumulated positive experience for obtaining high yields. Conclusions. Based on our own results and analysis of the experience of many countries around the world revealed the importance of legumes for our country. Their role in providing high-quality food, improving soil quality, increasing the country’s export potential is noted

Effect of pesticide treatment of spring wheat plots on seed yields

Introduction. High-quality varietal seeds, which can ensure a gain of 0.2–0.4 t/ha in the yields from their offspring, is one of the most important and cost-effective means to increase the gross grain collection of grain. Purpose. To study productive capacities of spring wheat seeds depending on treatment of fields with fungicides and insecticides. Materials and methods. The following indicators were evaluated: swelling activity, germination energy, laboratory germinability, initial growth strength, coleoptile length and the number of radicles. Seed samples that after pesticide treatment had showed the best results were tested in field experiments for productive capacities. The experimental plot area was 10 m2, in six replications. Seeds were sown with a seeder SN-10Ts after soybean with a seeding rate of 5 million germinable seeds per hectare. Spring wheat seeds produced in the experimental plots, which were treated with fungicides Akula (0.6 L/ha) and Soligor 425 EC (0.6 L/ha) and insecticides Fas (0.15 L/ha) and Karate Zeon 050 CS (0.15 L/ha) in organogenesis stages VI, VIII and X, were evaluated for productive capacities. The study was carried out on varieties MIP Zlata, Bozhena, MIP Raiduzhna and Diana. Results and discussion. Over the study years, the gain in the yields of spring wheat varieties grown from seeds of fungicide-treated plants was 0.23–0.36 t/ha; the gain in the yields of spring wheat varieties grown from seeds of insecticide-treated plants was – 0.24-0.31 t/ha. Pesticide-treated parental plants produced seeds with increased productive capacities: when such seeds were sown, the field germinability increased by 3-5%, and the plant survival – by 5-7%. Conclusions. The results indicate that the treatment of vegetating plants of spring wheat varieties in seed plots with fungicides and insecticides is a reliable way to obtain seeds with high productive capacities

Seed quality indicators of winter bread wheat accessions depending on the grain size

Purpose and objectives. To evaluate the effect of sorting seeds by size on their quality indicators: germination energy, laboratory germinability and 1000-grain weight. Materials and methods. The experiments were carried out in the experimental field of the Plant Production Institute named after V.Ya. Yuriev of NAAS in accordance with the field experimentation method in 2016–2018; 26 winter bread wheat lines and varieties were studied. The varieties and lines were grown in variety trial experiments; the plot area was 10 m2. Seeds were separated on sieves of a laboratory separator RLU-3; the 1000-grain weight was measured; the laboratory germinability and germination energy were determined. Results and discussion. When winter bread wheat seeds are sorted on sieves, they are separated into fractions with various 1000-grain weights. The separated fractions of winter wheat seeds have different sowing qualities. The 2.5 x 20 mm seeds and 2.8 x 20 mm seeds are of the best quality (germination energy, germinability). It was found that in 2016–2018, the germination energy, laboratory germinability and 1000-gain weight changed depending on the growing conditions. They were the highest in 2017. The varieties with a high 1000-grain weight have been identified: Doskonala, Pryvablyva, Smuhlianka, and Haiok. The highest germination energy and germinability were intrinsic to varieties Yednist, Harmonika and Bunchuk. Yednist (93.3%), Harmonika (94.3%), Metelytsia Kharkivska (94.5%), Bunchuk (94.7%), Alians (93.3%), Podolianka (92.5%), Patriotka (93.6%), Zapashna (93.3%), Dyvo (93.7%), Doskonala (93.6%), Krasa Laniv (94.0%), Smuhlianka (93.1%), and Erythrospermum 408-13 (93.7%) had a high germinability of small seeds (2.2 x 20 mm). To clean these varieties, especially in years with low seed yields, it is recommended to use seeds of the standard size (2.2–2.5 x 20 mm). Conclusions. The relationships between the grain size and sowing qualities of seeds have been established. Separation of seeds is an effective technological method allowing for selection of top-quality seeds

Productivity of mustachioed pea varieties depending on the nutrition background under the conditions of the eastern Forest-Steppe of Ukraine

The purpose of the research was to find out the effect of the main fertilization system in the crop rotation scheme on the yield and quality of seeds of moustachioed pea varieties in the Eastern Forest-Steppe of Ukraine. Materials and methods. Field studies were carried out in the period 2016–2020 in the stationary crop rotation scheme of V.Y.Yuriev Plant Production Institute of NAAS of Ukraine. The soil is powerful, poorly leached chernozem. The object of study were seven zoned varieties – Deviz, Tsarevych, Otaman, Oplot, Korvet, Haiduk and Malakhit. The experiment scheme included three backgrounds: 1 – crop rotation (without fertilizers); 2 – organic (manure aftereffect); 3 – organo-mineral (aftereffect of manure + N30P30K30 in the main application). The cultivation technology is generally accepted for the zone. The experimental results were statistically processed by the method of analysis of variance. Results and discussion. In unstable weather conditions 2016–2020 on the unfertilized background, the highest yield level was provided by the varieties Tsarevych (2.33 t/ha), Haiduk (2.28 t/ha) and Oplot (2.27 t/ha) with an excess of the standard variety Deviz by 0.19–0, 25 t/ha. Depending on the year of research, the maximum yield of these varieties varied within 3.04–3.21 t/ha, the minimum yield was 0.77–0.88 t/ha, and the range of variation was 2.27–2.38 t/ha. The productivity of the above mentioned varieties (2.75–2.77 t/ha) exceeded the standard by an average of 0.43–0.45 t/ha on the organic background, and compared with the unfertilized background, the increase in grain yield was 0.44–0.45 t / ha or 18.9–21.5 %. The highest yield (3.04–3.07 t/ha) and an increase in grain (0.77–0.84 t/ha) were provided by the varieties Tsarevych, Haiduk and Oplot on the organo-mineral background. This testifies to their good response to fertilizers and the possibility of cultivation using intensive technology. During the years of research, the maximum yield of these varieties was 4.16–4.39 t/ha, the minimum – 1.27–1.51 t/ha, and the highest range of its variation was from 2.79 t/ha to 2.89 t/ha. The smallest increase in grain on the organo-mineral background of fertilization was obtained in the varieties Malakhit and Korvet – 0.47 t/ha and 0.56 t/ha respectively. At the same time, the range of yield variation over the years was the smallest – 2.43–2.44 t/ha. The most favourable weather conditions for peas were in 2016, 2017 and 2020. Accordingly, this provided the highest level of average yield of varieties in terms of backgrounds: without fertilization – 2.70–2.72 t/ha, organic – 2.87–3.23 t/ha, organo-mineral – 3.14–3.83 t/ha. In the most unfavourable weather conditions of 2019, regardless of the nutritional background, the Tsarevych and Otaman varieties were more resistant to drought. The average yield of these varieties on the unfertilized background was 0.81 t/ha. At the same time, organic and organo-mineral nutritional backgrounds provided a significant increase in the grain – respectively 0.39 t/ha and 0.48 t/ha. Conclusions. On average for 2016–2020, the Tsarevych and Haiduk varieties were the most responsive to fertilization. The increase in grain of these varieties were consistently high – 0.44 and 0.47 t/ha on the organic background (aftereffect of manure) and 0.84 and 0.79 t/ha with the main application of N30P30K30 on the organic background respectively. On average, organic and organo-mineral fertilizer backgrounds for Tsarevych and Haiduk varieties ensured an increase in their productivity 0.38 t/ha and 0.69 t/ha respectively, with a yield under control (without fertilizers) of 2.21 t/ha. The dependence of the quality of pea grain on the variety and nutrition background was revealed. The highest protein content of grain was obtained in less productive varieties of Korvet and Malakhit with indicators, in the control of 24.09 and 23.35%, and on the organic-mineral background – 24.63% and 23.39% respectively. The possibility of the simultaneous increase in yield and protein content in the grain of the Haiduk variety has been established. The most significant gross protein harvest was obtained on the organic-mineral nutritional background in the Haiduk, Oplot and Tsarevych varieties (0.682–0.689 t/ha) with an increase of 16.8–17.8% in comparison with the standard variety Devyz. The increase in the gross yield of protein per hectare, in most varieties, depended on the level of their yield rather than on the protein content in the grain

Variability and genetic control of the “seedlings-earing” interphase period in spring barley under water deficit

Purpose. To determine the variability and genetic control of the “seedlings–earing” interphase period in spring barley under water deficit, to theoretically substantiate generations for selection in the diallel crossing design using cultivars bred at different breeding institutions and of different ecotypes, thereby solving the problem of shortening the spring barley breeding process. Material and methods. The study was conducted at Donetsk State Agricultural Station of NAAS of Ukraine. In 2018–2019, hybridization was performed and over 150 grains for each combination were obtained. In 2019–2020, the field experiments were laid out; cultivars and hybrids were sown within the optimal timeframe. The plots were arranged as per a P1 F1 P2 scheme. The row length was 1.5 m. A cassette seeder SKS-6-10 was used. The nutrition area was 10 cm x 20 cm. The experiments were carried out in three replications. The predecessor was black fallow. Data were processed using the package of applications for processing genetic and breeding experiments "EliteSystems gr." developed by the PPI nd.a. V.Ya. Yuriev NAASU. Based on genetic analysis, the Hayman parameters were determined. Results and discussion. The “seedlings–earing” interphase period in the cultivars varied 42.8 days to 49.1 days. In F1 hybrids, the “seedlings–earing” interphase period varied significantly, depending on crossing of cultivars of different ecotypes. Significant influence and SCA variance, the effects of allelic and non-allelic interactions were higher in a less favorable year: 18% vs. 14.5%. Analysis of the hybrid combinations indicates that the female effect, which increases the earing time, was seen in the hybrid combinations Partner/Komandor, Komandor/Baskak, Bohun/Baskak, and Bohun/Komandor. Baskak and Svarozhych reduced the earing time, while Komandor and Bohun increased it, and the GCA effects were most pronounced in the latter. Evaluation of the Wr–Vr difference homogeneity using t-test revealed no epistatic interaction (t = 0.21 and 0.10 insignificant). The regression line passes above the origin, indicating the leading role of dominance in the genetic control of the “seedlings–earing” period. This is confirmed by the indicator of medium degree of dominance. The divergence of the cultivar points along the regression line is significant, indicating the differentiation of the genotypes by the presence of dominant and recessive genes. In 2019, Partner and Komander were in the dominant zone, Baskak, Svarozhych and Bohun – in the recessive one. In 2020, Svarozhych and Komandor were in the recessive zone, and Bohun moved from the recessive zone to the dominant one. In general, the location of the cultivars along the regression line is relatively stable. In the loci that show dominance, the product of the frequencies of positive and negative alleles was asymmetric, and the ratio of the total number of dominant genes to the total number of recessive ones, proceeding from the values of >1, indicates prevalence of the former. In the genetic control of the “seedlings–earing” interphase period, incomplete intra-locus dominance and inter-locus additivity were recorded. Conclusions. Cultivars of different ecotypes bred at different breeding institutions, with due account for their competitiveness in terms of performance, were taken as initial components. Of crossing designs, we preferred diallel crossing for the following reasons: it allows obtaining the full range of combinatorics of the parents’ genetic information. The assemblage of F1 hybrids and parental cultivars gives a typical segregation. Starting selection, we have an idea about the trait inheritance and can determine from which generation to start it. Positive correlation coefficients between Wr + Vr and Xp (P3 = 0.32 ± 40 and 0.52 ± 0.32) indicate the stability of genetic systems determining the barley earing time, but they are insignificant, indicating the dominance direction, i.e. both dominant and recessive genes can reduce or increase this trait. The trait is controlled by a single genetic system, so selection can be based both on dominant alleles and on recessive ones, regardless of whether or not they reduce “seedlings-earing” interphase period. One should prefer recessive alleles, because they can be manifested in F2. Svarozhych can be used as a source to reduce the “seedlings-earing” interphase period, while Bohun can lengthen it

Performance inheritance and combining ability of spring barley accessions

Purpose and objectives. To establish the gene interaction types in the performance inheritance, to evaluate the combining abilities and effects of genes for this trait in spring barley accessions. Materials and methods. We investigated 75 F1 spring barley hybrid combinations derived from crossing 25 female forms with three male ones (chaffy and naked). Crossing was conducted in accordance with topcross design. F1 seeds and parents were sown with a cassette breeding planter SKS-6A. Grain pea was the forecrop. The plot area was 0.20 m2. The interrow distance was 0.20 m; the inter-plot tracks were of 0.50 m. Crossings were performed in two replications. Plants were harvested manually, with roots. For structural analysis, 20 typical plants were chosen from each F1 hybrid population, and the performance inheritance was determined by dominance degree (hp). The obtained data were grouped and gene interaction types were determined as per G.M. Beil and R.E. Atkins’s classification. Using two-factor analysis of variance in STATISTICA 10, we found significant differences between the GCA and SCA variances for the performance and evaluated the combining ability effects. Results and discussion. In 2019–2020, the features of 22 spring barley cultivars and three lines were described in terms of the gene interaction types in the performance inheritance and combining ability. On this basis, the prospects of biotype selections were evaluated. In the arid conditions of 2019, F1 only showed positive over dominance, while in favorable 2020 the inheritance types varied from positive to negative overdominance. In the two years, the general combining ability was high in female cultivars Khors and Troian. The male components did not show consistently high GCA for the both years. Hybrids between accessions with a high GCA and accessions with a lower or intermediate GCA may be promising in breeding due appearance of positive transgressions in the offspring. In 2019, the SCA effects were significantly strong in female forms Troian, Datcha, Gladys, Grace, Gatunok, Modern, and Herkules and in male forms Ahrarii and Scrabble. Conclusions. The study found that the gene interaction types in the spring barley performance inheritance depended on cross combinations and growing conditions. In unfavorable 2019, F1 only showed positive overdominance (heterosis), while in favorable 2020, positive overdominance, positive dominance and intermediate inheritance were observed. In 2019–2020, the high GCA was seen in female cultivars Khors and Troian. The strong SCA effects were noticed in female components Troian, Datcha, Gladys, Grace, Gatunok, Modern, and Herkules and male forms Agrarii and Scrabble. The hybrid combinations with the maximum probability of producing transgressive segregants have been selected

Effect of thickening of watermelon sowing on yield and seed quality

Purpose and objectives. To study the effect thickening plantations of watermelon seed plants on seed yield and quality, using different thickening designs in the Northern Steppe of Ukraine. Materials and methods. The main crop was watermelon. Sweet corn and green bean were used as thickeners. The thikeners were placed in watermelon interrows or in rows with the following layouts: 1.4 m x 1.0 m and 2.8 m x 1.0 m (sweet corn) and 1.4 m x 0.5 m, 2.8 m x 0.5 m (green beans). Results and discussion. The highest output of seeds per fruit (21.3 g) was recorded when sweet corn was sown between watermelon rows with a layout of 2.8 m x 1.0 m (18.4 g in the control). Growing sweet corn in watermelon interrows increased the 1000-seed weight from 52.6 g (control) to 55.5 g. The highest yield of watermelon seeds (187 kg/ha) was obtained in plantations where sweet corn was grown watermelon interrows with a layout of 2.8 m x 1.0 m (+ 21 kg/ha or 12.7% to the control). The use of green bean as a thickener for watermelon was less effective. The yield increased by 7-15 kg/ha (4.2–9.0%) compared to the control. In addition, 0.8-1.8 t/ha of milky-wax cobs of sweet corn and 47–98 kg/ha of green beans were harvested. Conclusions. The use of thickeners grown between rows of the main crop is the most effective technological technique in the Northern Steppe of Ukraine to increase the yield and improve the quality of watermelon seeds. Our experiments demonstrated that sweet corn was the best thickener, interrow was the best placement, and 2.8 m x 1.0 m was the best layout. This method in watermelon seed production allows increasing the seed yield by 12.7% related to the control (no thickeners) and raising the economic efficiency of seed production due to a gain in the net profit of 14,800 UAH/ha (26.4%) and profitability by 37.9% while maintaining high sowing indicators of seeds

Inheritance of the performance and its constituents by common bean (Phaseolus vulgaris L.) hybrids and lines

Purpose. To study the inheritance nature and polymorphism of the performance as well as the traits determining it in intervariety hybrids of common bean (Phaseolus vulgaris L.) and to identify promising genotypes and lines for further practical breeding. Material and methods. The intraspecies F1–F6 hybrid common bean (Phaseolus vulgaris L.) combinations derived from crosses of varieties of different eco-geographical origin (Mavka/CDC Camino, Belko/CDC Rosalee and Mavka/Niger Wally) were studied. The study object was the performance and suitability for mechanized harvesting as well as traits that determine them. The breeding was conducted according to the full breeding design. We conducted multiple individual selections, starting with F2. We used experimental-field and laboratory-analytical methods and statistical processing of data on a PC. Results and discussion. The degree of phenotypic dominance of the plant height in F1 hybrids varied 9.4 to –1.75, of the performance and its constituents – 2.7 to – 1.5. The frequency of probable transgressions in F2 was within 16.5–32.0 and 0–14.7%, respectively, with a coefficient of variation ranging 17.5% to 61.9%. The index traits were less variable. In the F3 Mavka/CDC Camino the share of performance-positive transgressions was 50.0%; in the F3 Belko/CDC Rosalee it was 12.0% related to the number of transgressive forms in F2. In the F4 Mavka/CDC Camino, it increased to 66.1%. 57.1% of the F5–F6 breeding lines gave higher yields than that from check variety Pervomaiska. In 64.3% of the breeding lines, the yield stability exceeded 70%; 39.2% of the accessions were better (81.4–97.1%) than the check variety (76.5%). Conclusions. Intravariety hybridization was proved to be an effective method for the crop breeding. It is recommended to select idiotypes from hybrid populations from crossing forms have contrasting traits, starting with F3; it is advisable to use the harvest index as a selection criterion

Influence of macro- and micro-fertilizers on biometry, performance and qualitaty of sunflower hybrids

Sunflower is a crop requiring intensive mineral nutrition, therefore, its cultivation demands reserves of nutrients in the soil, which can be replenished by applying mineral fertilizers. Purpose. Our purpose was to study the effects of fertilizers on the growth, development and yield of sunflower hybrids of different ripeness groups (bred at the Institute of Oil Crops) in the Left-Bank Forest-Steppe of Ukraine. Material and methods. The study was conducted at Poltava State Agricultural Research Station named after N.I. Vavilov in 2019–2020 The study object was processes of the growth and development of sunflower plants and formation of sunflower seeds depending on farming techniques. The study subject was sunflower hybrids Ahronomichnyi, Ahent and Serpanok. The following fertilizers were applied: 1) no fertilizers (control); 2) N32Р32К32; 3) N32Р32К32 + foliar fertilization of plants with urea (10 kg/ha) in the phase of 5–6 pairs of leaves; 4) N32Р32К32 + foliar fertilization of plants with microfertilizer Novalon Foliar (1 kg/ha) in the phase of 2–3 pairs of leaves; 5) N32P32K32 + foliar fertilization of plants with microfertilizer Novalon Foliar (1 kg/ha) in the phase of 5–6 pairs of leaves. Results and discussion. The results show that mineral macro- and microfertilizers contributed to more intensive growth and development of plants. Thus, the tallest plants, the largest leaves and calathidiums, and the highest 1000-seed weight were recorded hybrids Ahronomichnyi, Ahent and Serpanok with basic mineral fertilizers at a dose of N32Р32К32 + foliar fertilization with carbamide (10 kg/ha) or with microfertilizer Novalon Foliar (1 kg/ha). The maximum yields from hybrids Ahronomichnyi, Ahent and Serpanok were achieved with basic mineral fertilizers N32Р32К32 + foliar fertilization with microfertilizer Novalon Foliar (1 kg/ha) – 3.35 t/ha, 3.41 t/ha and 3.15 t/ha respectively. The gain to the control (no fertilizers) was 0.33 t/ha, 0.32 t/ha and 0.22 t/ha, respectively. Fertilization also affected the oil content in seeds. Mineral fertilizers N32Р32К32 decreased the oil content in the hybrid seeds by 1.1–1.7% compared to the ‘no fertilizers’ experiment. However, fertilization of plants during the growing period with urea or microfertilizer Novalon Foliar in combination with basic mineral fertilizers N32Р32К32 significantly increased the oil content in seeds. With the best yields, the highest output of oil from the hybrids was 1,406 kg/ha, 1,446 kg/ha and 1,356 kg/ha, respectively. Conclusions. Our results mainly confirm the results of other studies. Mineral fertilizers and microfertilizers promote more intensive growth and development of plants and increase the yields of sunflower seeds. However, there are some discrepancies in the oil content in seeds. Some researchers reported that mineral fertilization increased the oil content in seeds compared to the control (no fertilizers). We found that application of mineral fertilizers at a dose of N32P32K32 caused a decrease in the oil content in seeds, and only supplementary foliar fertilization with urea or microfertilizers increased this indicator

Environmental trial of pea breeding acessions

The aim of the study was to determine the information content and adequacy of the ecological testing points of the pea breeding material and to identify the "ideal" genotype. Materials and methods. The breeding material in the experiments was represented by cultivars bred at the PPI nd.a. V.Ya. Yuriev (Tsarevych, Oplot, Otaman, Metsenat, Korvet, Haiduk, and Malakhit) and ten breeding lines (SL 11-129, SL 11-213, SL 11-55, SL 11-58, SL 10-37, SL 11-32, SL 10-132, SL 09-118, SL 11-166, and SL 11-176). In addition there was one cultivar (Svit) bred at the Plant Breeding and Genetics Institute. All the cultivars are leafless, semi-dwarf, mid-ripening, except for Tsarevych (mid-early). The field experiments were carried out in accordance with the methods of field experimentation, using the conventional pea growing technology. The seeding rate was 1.2 million germinable seeds/ha; the plot area was 10 m2. To evaluate the accessions for the variability in different environments, we used a regression model developed by S.A. Eberhart and W.A. Russel, where the regression coefficient is an indicator of the genotype-environment interaction. This model is included in "Guidelines for Environmental Trials of Corn". Results and discussion. Thus, comparing the regression coefficient in pea cultivars Oplot, Tsarevych, Haiduk, Korvet, and Metsenat, we could conclude that these accessions were highly intensive in the OSES conditions and extensive in the PPI NAAS conditions (except for Metsenat). Regarding the regression coefficient in the breeding lines, none of them had a regression coefficient of 1.0. Over the study period, the regression coefficient was 1.4 only in line SL 11-58 (PPI NAAS) and 1.2 (OSES), characterizing this line as intensive regardless of the place of cultivation. Taking into account that the regression coefficient values of <1 are intrinsic to extensive accessions, lines SL 10-132 (RC = 0.4) and SL 11-176 (RC = 0.8) are preferred. Because these accessions also have a high genotypic effect. In addition, the regression coefficient in breeding line SL 09-118 was 0.9, with a genotypic effect of 0.07. Such combination of the indicators characterizes the line as relatively stable, with sufficient potential performance, and this breeding line will not be demanding to growing conditions similar to the OSES ones. Conclusions. Thus, the evaluation of both cultivars and breeding lines in the environmental trial showed that the pea breeding at the Plant Production Institute named after VYa Yuryev had a significant potential to create cultivars that would be well-adapted to both eastern and southern conditions, and that environmental trials remained an effective tool for assessing breeding material and selecting accessions with the maximum fulfillment of the genetic potential