Factors Contributing to the Persistence of E. coli in the Human Large Intestinal Microflora

1991 ◽  
pp. 331-333
Author(s):  
Agnes E. Wold ◽  
Dominique Caugant ◽  
Gunilla Lidin-Jansson ◽  
Peter de Man ◽  
Catharina Svanborg
Keyword(s):  
Intestinal Microflora ◽  
E Coli

Correlation Between Intestinal Microflora and Gastrointestinal Dysfunction in Elderly Patients with Sepsis

2020 ◽  
Vol 12 (8) ◽  
pp. 1030-1037
Author(s):  
Hailing Yang ◽  
Xiaolin Zhang ◽  
Weijun Chen ◽  
Libo Shang ◽  
Bin Chen ◽  
...  
Keyword(s):  
Elderly Patients ◽  
Intestinal Microflora ◽  
Sepsis Group ◽  
Control Group ◽  
Gastrointestinal Dysfunction ◽  
E Coli ◽  
Significant Difference ◽  
Prevention And Treatment ◽  
The Common ◽  
Wbc Count

Multiple organ dysfunction syndrome (MODS) is a key factor that leads to death in elderly patients with sepsis. Therefore, early prevention and treatment of gastrointestinal dysfunction (GIDF) in elderly patients with sepsis is an important measure to prevent MODS occurrence. This research explores the correlation between intestinal microflora and GIDF in elderly patients with sepsis and provides ideas for the prevention and treatment of GIDF in elderly patients with sepsis. In this study, 152 patients with sepsis (122 patients with sepsis and GIDF) treated in the Third Affiliated Hospital of Yunnan University of Chinese Medicine from January to September 2019 were selected as the sepsis group and 100 elderly who had normal physical examination results were selected as the control group. The common intestinal microflora of the two groups was compared. Patients with sepsis and GIDF were treated as the GIDF group and the other patients with sepsis were treated as the non-GIDF group. The common intestinal microflora, gastrointestinal indicators, serum inflammatory factors, and immune function indices were compared between the two groups. Correlation analysis of the observed indices with statistical significance was carried out. The results showed 152 patients with sepsis and 122 patients with sepsis and GIDF; thus, the incidence of sepsis with GIDF was 80.26%. The total average score of sepsis with GIDF was 3.61±0.09. There was no statistically significant difference in GIDF scores of patients ages 65–75 and > 75 years old. The number of Bifidobacterium and Lactobacillus in elderly patients with sepsis was lower and the number of Escherichia coli was higher than in the control group. In elderly patients with sepsis, the number of Bifidobacterium and Lactobacillus in the GIDF group was lower and the number of E. coli was higher than in the non-GIDF group. White blood cell (WBC) count, procalcitonin (PCT), C-reactive protein (CRP), tumor necrosis factor-α (TNF-α), gastrin (GAS), and diamine oxidase (DAO) in GIDF patients were higher and motilin (MOT), CIT (CIT), CD4+, and CD8+ were lower than in the non-GIDF group. WBC count, PCT, CRP, TNF-α, GAS, and DAO were negatively correlated with the number of Bifidobacterium and Lactobacillus but positively correlated with E. coli. MOT, CIT, CD4+, and CD8+ were positively correlated with the number of Bifidobacterium and Lactobacillus but negatively correlated with E. coli. There was a negative correlation between Bifidobacterium and Lactobacillus and GIDF score and a positive correlation between E. coli and GIDF score. Therefore, the change in the intestinal microflora in elderly patients with sepsis is related to GIDF.

scholarly journals Is there any function for colicinogeny in the post-weaning diarrhoea of piglets?

2012 ◽  
Vol 48 (No. 7) ◽  
pp. 190-198
Author(s):  
J. Trcka ◽  
J. Smarda
Keyword(s):  
Intestinal Tract ◽  
Intestinal Microflora ◽  
Weaned Piglets ◽  
E Coli ◽  
Decline Phase ◽  
Commensal Flora ◽  
Pathogenetic Factor ◽  
Experimental Approaches ◽  
Colicin V

Using seven experimental approaches, we attempted to solve the question of possible participation of colicinogeny and colicin-sensitivity in the pathology of the post-weaning diarrhoeic enteritis of piglets. In our research, both enterotoxic E. coli strains (ETEC) and normal, commensal E. coli strains in the intestinal microflora of 803 weaned piglets were followed. In diarrhoeic piglets, colicinogeny was more frequent in the ETEC strains than in the simultaneously isolated commensal ones. ETEC strains were largely insensitive to the most frequently appearing colicin types and the inhibitive effect of their colicins on commensal E. coli was less likely than the opposite (inhibition of ETEC strains by colicins of the commensal ones). Bolstering the diet of healthy piglets with a mixture of symbiotic colicinogenic strains and colicin-sensitive ETEC strains could not prevent diarrhoeic enteritis due to established dominance of ETEC. In all parts of the intestinal tract during the post-weaning diarrhoea (PWD), mostly non-colicinogenic strains of the commensal flora survived. In six serotypes of ETEC strains, the frequency of colicinogenic strains ranged from 7% (in the serotype O139) to 66% (in serotype O141). From 9 frequent colicin types, colicin V (mainly against the serotypes O8 and O147), E2 (against O139 and O8), D (mainly against O8) and E3 (mainly against O138) met most sensitive strains among ETEC. Hence, colicinogeny was no pathogenetic factor of PWD. Nevertheless, colicinogenic commensal strains gradually regained dominance during the decline phase of the disease in surviving piglets.

scholarly journals Rape-seed meal toxicity in gnotobiotic rats: influence of a whole human faecal flora or single human strains ofEscherichia coliandBacteroides vulgatus

1993 ◽  
Vol 70 (1) ◽  
pp. 323-331 ◽  
Author(s):  
Sylvie Rabot ◽  
Lionelle Nugon-Baudon ◽  
Pierre Raibaud ◽  
Odette Szylit
Keyword(s):  
Plasma Levels ◽  
Intestinal Microflora ◽  
Diet Group ◽  
Seed Meal ◽  
Control Group ◽  
Rape Seed ◽  
Faecal Flora ◽  
E Coli ◽  
Bean Meal ◽  
Soya Bean Meal

Gnotobiotic growing rats harbouring either a whole human faecal flora or single human strains ofEscherichia coli(EM0) orBacteroides vulgatus(BV8H1) were fed for 7 weeks on semi-synthetic diets in which the protein source was either soya-bean meal (SM) or rape-seed meal (RM). For each bacterial status the RM-diet group was compared with the control group fed on the SM diet. The association of human faecal flora with the RM diet was responsible for reduced feed intake and reduced weight gain, an enlargement of the liver and thyroid and a decrease in both thyroxine and triiodothyronine plasma levels. The association of theB. vulgatusBV8H1 strain with the RM diet reproduced all these effects, except that triiodothyronine plasma levels were not significantly modified. Rats inoculated with theE. coliEM0 strain and fed on the RM diet exhibited a goitre and lowered thyroxine and triiodothyronine plasma levels. These results show that the human intestinal microflora may be involved in glucosinolate metabolism when cruciferous vegetables are consumed by man. The specificity of the symptoms observed according to the rat bacterial status supports the hypothesis that bacteria yield specific toxic glucosinolate derivatives according to their enzymic potential.

scholarly journals THE CORRECTIVE EFFECT OF CAROTENEPRODUCED YEAST PHAFFIA RHODOZYMA BIOMASS ON INTESTINAL MICROBIOCENOSIS FORMATION OF DUCKS

2016 ◽  
Vol 23 ◽  
pp. 30-35
Author(s):  
M. V. Kaminska ◽  
О. М. Stefanyshyn ◽  
A. V. Guntchak
Keyword(s):  
Growth And Development ◽  
Intestinal Microflora ◽  
Phaffia Rhodozyma ◽  
Critical Periods ◽  
E Coli ◽  
Corrective Effect ◽  
Poultry Diets ◽  
Experimental Group ◽  
Intestinal Microbiocenosis

The changes the of intestinal microflora composition of Beijing broiler ducks at introduction of caroteneproduced yeast Phaffia rhodozyma biomass to the diet was studied. The positive changes of 37-days ducks intestinal microflora were revealed after two weeks of yeast P. rhodozyma biomasses application in 6- day-old birds’ diet (1 % of the weight of feed). It was a decrease of the E. coli total number and pathogenic staphylococci strains, the absence of Enterobacteriaceae (lac–) strains against the high number of bifidobacteria and lactobacilli. The E. coli total number reduced by 10 times at the expense of E. coli (lac±)-strains compared to 37-day birds three weeks after the experiment. The staphylococcus non-pathogenic strains number in 58-days ducks experimental group caecum contents reduced compared to 37-day birds three weeks after the experiment. Thus, the using of caroteneproduced yeast P. rhodozyma biomass in poultry diets helps to prevent violations of intestinal microflora and occurrence dysbiosis at critical periods of growth and development.

Sesamin Modulation of Intestinal Microflora in Rats on High-Fat Diet

2020 ◽  
Vol 19 (3) ◽  
pp. 312-316
Author(s):  
Pan Liu ◽  
Yu Deng ◽  
Mengxi Wang ◽  
Lingyan Kong ◽  
Na Xu ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Intestinal Microflora ◽  
High Throughput Sequencing ◽  
Intestinal Flora ◽  
Short Chain Fatty Acids ◽  
Sequencing Analysis ◽  
Anaerobic Culture ◽  
High Fat ◽  
E Coli

The present study aimed to investigate the effects of sesamin on intestinal flora in rats on high-fat diet by in vitro animal fecal anaerobic culture system. Results showed that treatment with sesamin increased the abundance of Lactobacillus acidophilus and inhibited the growth of E. coli. High throughput sequencing analysis showed that treatment with sesamin increased the abundance of probiotics such as Lactobacillus and Flavonifractor, and decreased the abundance of harmful bacteria such as Bacteroides, Enterococcus, Clostridium_XlVa, and Parabacteroides. Moreover, treatment with sesamin increased the concentration of total short chain fatty acids (acetic acid and isovaleric acid). In conclusion, sesamin intervention improved the composition and abundance of intestinal microorganisms of high fat diet rats, which would be beneficial to intestinal health.

scholarly journals Effect of the Addition of Magnesium Salt to a Feed Mixture on Intestinal Microflora, Health, and Production of Sows

2013 ◽  
Vol 57 (1) ◽  
pp. 69-72 ◽  
Author(s):  
Beata Trawńska ◽  
Antoni Polonis ◽  
Jerzy Lechowski ◽  
Leszek Tymczyna ◽  
Ryszard Borowski ◽  
...  
Keyword(s):  
Body Weight ◽  
Survival Rate ◽  
Reproductive Performance ◽  
Intestinal Microflora ◽  
Alimentary Tract ◽  
Magnesium Salt ◽  
Control Group ◽  
Feed Mixture ◽  
E Coli ◽  
Newborn Piglets

Abstract The effect of feed mixture supplementation with MgCl2 on alimentary tract microflora, haematological markers, and selected parameters of reproductive performance of sows was investigated. The animals were divided into control group (K) and two experimental groups (E1 and E2). The sows from the experimental groups were receiving 1 g of MgCl2 6H20/100 kg b.w./day, which constituted 120 mg of pure magnesium, administered in a small portion of a feed mixture. The sows from group E1 were receiving MgCl2 every day for 30 d, whereas sows from group E2 for 60 d. A significant decrease in the count of E. coli, Providencia sp., and Proteus sp. was noted in faeces of the swine of both experimental groups. The study has also demonstrated a reduced number of lymphocytes and an increased number of granulocytes in blood of the sows receiving MgCl2. Feeding of pregnant sows with the addition of magnesium salt decreased the mortality rate of newborn piglets, increased the survival rate of piglets until 21 d of their life, and increased body weight values of litters originating from experimental sows, compared to the litters of control sows.

scholarly journals Plasmon-Enhanced Antibacterial Activity of Chiral Gold Nanoparticles and In Vivo Therapeutic Effect

Nanomaterials ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1621
Author(s):  
Yuelong Xu ◽  
Hongxia Wang ◽  
Min Zhang ◽  
Jianhao Zhang ◽  
Wenjing Yan
Keyword(s):  
Gold Nanoparticles ◽  
Antibacterial Activity ◽  
Intestinal Microflora ◽  
Antibacterial Properties ◽  
Au Nps ◽  
E Coli ◽  
Bacterial Cell Death ◽  
Steric Configuration

d-cysteine (d-cys) has been demonstrated to possess an extraordinary antibacterial activity because of its unique steric configuration. However, inefficient antibacterial properties seriously hinder its wide applications. Here, cysteine-functionalized gold nanoparticles (d-/l-Au NPs) were prepared by loading d-/l-cysteine on the surface of gold nanoparticles for the effective inhibition of Escherichia coli (E. coli) in vitro and in vivo, and the effects on the intestinal microflora in mice were explored during the treatment of E. coli infection in the gut. We found that the antibacterial activity of d-/l-Au NPs was more than 2–3 times higher than pure d-cysteine, l-cysteine and Au NPs. Compared with l-Au NPs, d-Au NPs showed the stronger antibacterial activity, which was related to its unique steric configuration. Chiral Au NPs showed stronger destructive effects on cell membrane compared to other groups, which further leads to the leakage of the cytoplasm and bacterial cell death. The in vivo antibacterial experiment illustrated that d-Au NPs displayed impressive antibacterial activity in the treatment of E. coli-infected mice comparable to kanamycin, whereas they could not affect the balance of intestinal microflora. This work is of great significance in the development of an effective chiral antibacterial agent.

scholarly journals Effect of Adding Broccoli Leaves (Brassica oleracea L.) Extract to Drinking Water on Eggs Production and Intestinal Microflora of Japanese Quail Coturnix japonica Temmink & Schlegel, 1849

2020 ◽  
Vol 33 (2) ◽  
pp. 42-51
Author(s):  
Duha S.A. Al-Ashoor ◽  
Khalid C.K. Al-Salhie
Keyword(s):  
Drinking Water ◽  
Intestinal Microflora ◽  
The Other ◽  
Feed Consumption ◽  
Alcoholic Extract ◽  
Feed Conversion ◽  
E Coli ◽  
The Third ◽  
Japanese Quails ◽  
Males And Females

This study was carried out to determine the effects of adding broccoli leaves extract to drinking water on eggs production and intestinal microflora (total bacteria, lactobacilli and Escherichia coli) of breeder Japanese quails. One hundred and thirty-two 45 days-old of Japanese quails (males and females) were randomly distributed into four groups. Each group has three replicates. The groups were included: The first group was kept without any addition as the control. The extraction of broccoli leaves as 100, 200 and 300 mg. l-.1 were added to bird's drinking water of the second, third and fourth groups respectively. The results showed a significant improvement in eggs production (HD %), accumulative eggs per 30 days and eggs mass in the third and fourth groups compared to the other groups. While, no significant differences were shown in the feed consumption, feed conversion ratio and eggs weight among groups. Caecal and duodenum E. coli showed a significant decrease in third and fourth groups compared to the others. However, duodenum lactobacilli increased significantly in the fourth group compared to other groups. It was also showed no significant differences in the total duodenum bacteria among studied groups. While, the total caecal bacteria decrease significantly in the third group compared to other groups. Based on the presented results can be concluded that alcoholic extract of broccoli leaves at levels 200 and 300 mg. l-1 could enhance productive characteristics (eggs production HD %, accumulative eggs per 30 days and eggs mass). On the other hand, it increased duodenum lactobacilli and decreased caecal and duodenal E. coli  population of breeder Japanese quails.

The ability of Salmonella enterica to biofilm formation in conditions of interspecific interaction with representatives of the intestinal microflora

2021 ◽  
pp. 75-80
Author(s):  
E.V. Matosova
Keyword(s):  
Optical Density ◽  
Intestinal Microbiota ◽  
Salmonella Enterica ◽  
Biofilm Formation ◽  
Intestinal Microflora ◽  
Gentian Violet ◽  
Density Measurement ◽  
Interspecific Interaction ◽  
E Coli

For effective distribution in the gastrointestinal tract, Salmonella must have the ability to form biofilms and compete for nutrients with the host microbiota. Objective: to characterize the ability of Salmonella enterica Typhimurium to biofilm formation under conditions of interspecific interaction in a multicultural biofilm with representatives of the intestinal microbiota in an in vitro experiment. Museum strains of the bacteria Salmonella enterica serovar Typhimurium, Lactobacillus acidophilus and Lactobacillus casei, Escherichia coli were used in the work. The process of interspecific interaction was modeled in a mixture of LB-broth with 0.85 % NaCl solution in a ratio of 1:3 at an initial bacterial concentration of 10³ CFU / ml in Petri dishes with a diameter of 65 mm at a temperature of +37 °C. The experimental results were evaluated within 12 days. The plate method was used to count the number of viable cells in biofilms. The optical density of the matrix of the biofilm stained with gentian violet was measured using spectrophotometry (wavelength 595 nm). To assess tinctorial properties, the bacterial samples were Gram-stained.. Enzymatic properties were assessed using Giss media and differential diagnostic media. All experiments were repeated 3 times. It was found that Salmonella enterica Typhimurium exhibit the ability to form biofilm. In the initial stages of biofilm formation, the bacteria of the intestinal microbiota suppress the development of Salmonella. In mature mixed biofilms, the growth of S. enterica and E. coli is mutually stimulated. By the end of the experiment, the dominance of Salmonella over the intestinal microbiota was noted. The results of the optical density measurement suggest the presence of species-specificity in bacterial interaction. Conclusion. Intestinal microbiota bacteria inhibit the growth of Salmonella bacteria in biofilms only in the initial stages. In mature mixed biofilms, the growth of E. coli and S. enterica is mutually stimulated, however, on the 12th day, Salmonella dominates over E. coli and Lactobacillus spp. (p > 0.05). English version of the article is available at URL: https://panor.ru/articles/ability-of-salmonella-enterica-to-biofilm-formation-under-conditions-of-interspecies-interaction-with-representatives-of-the-intestinal-microflora/72525.html

scholarly journals Influence of the type of housing on the intestinal microflora of layers and broilers

2021 ◽  
Vol 273 ◽  
pp. 02029
Author(s):  
Maria Mazanko ◽  
Eugenia Prazdnova ◽  
Anjelika Bren ◽  
Dmitriy Rudoy ◽  
Arkady Babajanyan
Keyword(s):  
Gut Microbiota ◽  
Intestinal Microflora ◽  
Intestinal Bacteria ◽  
Young Age ◽  
Poultry House ◽  
E Coli ◽  
Free Range ◽  
Number Of Bacteria

The microbiota of birds of different housing types was studied, namely: cage, outdoor and free-range. The number of bacteria Lactobacillus, Bifidobacterium, Enterococcus, E. coli and lactose-positive, Bacillus in bird faeces was investigated. It has been shown that the composition of the microbiota does not depend on the type of their content but depends on the type of nutrition and on the microbiota that was initially located in a farm or poultry house environment. At the same time, the microbiota of birds can change at a young age, but after puberty, it becomes more stable. The microbiota of free-range birds remained stable while maintaining diet and changed with diet changes depending on the season. It has also been shown that litter and soil are capable of retaining significant numbers of intestinal bacteria. Bacillus can colonize birds’ gut, but it is not a permanent participant in the gut microbiota of birds.

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