scholarly journals THE EFFECTS OF CHRONIC STRESS ON THE MORPHOLOGICAL CHARACTERISTICS OF TESTICULAR TISSUES OF WISTAR RATS

2017 ◽  
pp. 66-69
Author(s):  
K. A. Kidun ◽  
E. K. Solodova ◽  
T. S. Ugolnik
Keyword(s):  
Basement Membrane ◽  
Chronic Stress ◽  
Reproductive System ◽  
Wistar Rats ◽  
Morphological Changes ◽  
Morphological Characteristics ◽  
Male Reproductive System ◽  
Seminiferous Epithelium ◽  
Seminiferous Tubules ◽  
Unfavorable Effect

Stress has an unfavorable effect on male reproductive system. The aim of the work was to study the morphological changes in testicular tissues of Wistar rats in chronic stress according to Ortiz. We detected hyperemia of vessels, reduction of the diameter of seminiferous tubules, exfoliation of the seminiferous epithelium from the basement membrane, and also destructive changes in the seminiferous epithelium including development of necrosis in the testis of the rats after stress.

scholarly journals Red-Fleshed Apple Anthocyanin Extracts Attenuate Male Reproductive System Dysfunction Caused by Busulfan in Mice

2021 ◽  
Vol 8 ◽  
Author(s):  
Jihua Xu ◽  
Xiang Zhang ◽  
Xiaohong Sun ◽  
Qiang Lv ◽  
Yugang Zhang
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Oral Administration ◽  
Reproductive System ◽  
Sperm Concentration ◽  
Male Reproductive System ◽  
Seminiferous Tubules ◽  
Tandem Mass ◽  
Treatment Groups ◽  
Positive Effects

In this research, we analyzed the effect of an intragastrical oral administration of red-fleshed apple anthocyanin extract (RAAE) on busulfan-treated mice. First, we showed that the most abundant component in RAAE was cyanidin 3-O-galactoside. To determine the effect of the RAAE, the mice were divided into control and four other different concentrations of RAAE feeding treatment groups (BA0, no RAAE; BA.1, 0.1 mg/kg; BA1, 1 mg/kg; and BA5, 5 mg/kg) following busulfan injection. We observed that RAAE treatments displayed ameliorative effects on male reproductive system dysfunction caused by busulfan, such as recovering the irregular arrangements of seminiferous tubules, increasing the number of spermatogonia and spermatocytes, improving sperm concentration by 3-fold in BA.1, and improving sperm motility by 2-fold in BA1. The liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis showed significant up- or downregulation of certain metabolites, such as lysophosphatidylcholine (LysoPC), L-arginine, glycine, anandamide, and L-carnitine, which could contribute to the positive effects of RAAE, especially in PBA1 (plasma of BA1) and PBA5 (plasma of BA5). Taken together, the results indicate that 1 mg/kg of RAAE is a suitable concentration for rescuing spermatogenesis in mice. The research suggests that RAAE could be a potential nutraceutical for protecting spermatogenesis after busulfan therapy in cancer.

scholarly journals METRONIDAZOLE INDUCES SIGNIFICANT PATHOLOGICAL ALTERATIONS IN THE MALE REPRODUCTIVE SYSTEM OF MICE

2021 ◽  
Vol 52 (6) ◽  
pp. 1375-1381
Author(s):  
Z. K. Al – Timimi
Keyword(s):  
Reproductive System ◽  
Sperm Morphology ◽  
Male Reproductive System ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Distilled Water ◽  
Histopathological Changes ◽  
Negative Effects ◽  
The Mean ◽  
Water Treated Control

The present study was aimed to investigate the possible effects caused by the antibiotic, metronidazole, on the histology of the testis and the morphology of the sperms of mice. To achieve this,  adult male albino Swiss Mice were orally administrated with 0.1 ml (100 mg\ kg ) of the commercially used metronidazole (Flagyl) for 60 consecutive days. The results showed significant decreases (p<0.05) in the mean weight of body and testis in the treated mice as compared to the distilled water-treated control group. The treatment also caused several histopathological changes in the testis which included necrosis, congestion, hemorrhage, edema, germ cell downfall  and rupture in the seminiferous tubules. The tests of the sperm morphology revealed a number of deformations as a result of the treatment, such as the appearance of headless,  bend, broken-tailed, hook-tailed, and two-headed sperms, as well as sperms with cytoplasmic droplets. We conclude that from these results that orally administered Metronidazole exerts negative effects on the male reproductive system of mice .

scholarly journals Effect of Diabetes on the Male Reproductive System—A Histomorphological Study

2019 ◽  
Vol 36 (01) ◽  
pp. 017-023 ◽  
Author(s):  
Sushma Kotian ◽  
Ashok Kumar ◽  
Sanchari Mallik ◽  
Nandini Bhat ◽  
Anne Souza ◽  
...  
Keyword(s):  
Reproductive System ◽  
Histological Study ◽  
Autoimmune Disorder ◽  
Diabetic Rats ◽  
The Body ◽  
Male Reproductive System ◽  
Diabetic Group ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Minimum Diameter

Introduction Type 1 diabetes is an autoimmune disorder characterized by lack of insulin production by the β cells of the pancreas. This lack of insulin causes a variety of systemic effects on the metabolism of the body, one of which is reproductive dysfunction. The present study investigates the effects of diabetes on the male reproductive system of streptozotocin (STZ)-induced diabetic rats. Material and Methods A total of 18 adult male Wistar rats weighing ∼ between 250 and 300 g were included in the present study. The animals were divided into normal and diabetic groups. The diabetic group was further subdivided into 2 subgroups with durations of 24 and 48 days. A single dose of STZ (40 mg/kg body weight) was administrated intraperitoneally to the animals of the diabetic group. After the planned duration, the testes and epididymides were dissected, and their gross weight was measured. The tissues were then processed for histological study. Results The gross weight of the testes and epididymides in diabetic rats at 24 and 48 days showed a decrease in comparison to the control. (p < 0.01 for testes and epididymides).Diabetic animals presented a significant decrease in the diameter of the seminiferous tubules compared with the control group (p < 0.01). The epididymides in the diabetic groups showed a considerable reduction in the tubular surface area compared with the control group (p < 0.01). There was also a reduction in the mean diameter, which was measured using the maximum and minimum diameter of the tubules (p < 0.01). Conclusion The present study is an insight into the adverse effects that diabetes can have on the tissue structure of the testes, of the epididymides, and ultimately on the process of spermatogenesis.

scholarly journals Spermiogenesis, Stages of Seminiferous Epithelium and Variations in Seminiferous Tubules during Active States of Spermatogenesis in Yangzhou Goose Ganders

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 570
Author(s):  
Muhammad Faheem Akhtar ◽  
Ejaz Ahmad ◽  
Sheeraz Mustafa ◽  
Zhe Chen ◽  
Zhendan Shi ◽  
...  
Keyword(s):  
Reproductive Performance ◽  
Morphological Changes ◽  
Seminiferous Epithelium ◽  
Poultry Meat ◽  
Seminiferous Tubules ◽  
Germplasm Resources ◽  
High Productivity ◽  
The Past ◽  
The World ◽  
Tubular Diameter

The past three decades revolutionized the goose industry in the world. China holds the world’s largest goose breeds stock by 95% of the global total. To optimize the goose industry and cope with ever increasing poultry meat and egg demands, there is a dire need to focus on reproduction, as most geese breeds exhibit poor reproductive performance. The present study was conducted with the aim to add a contribution in the goose industry and research by the histological visualizing step wise development of germ cells during spermatogenesis by microscopy and a histological technique. Yangzhou goose is a synthetic breed developed by using local goose germplasm resources of China. It is popular in the Chinese goose industry due to high productivity and adaptability. This research evaluated the steps of spermiogenesis and stages along with morphological changes in the seminiferous epithelium in Yangzhou goose ganders. For the assessment of various stages of the seminiferous epithelium cycle, testis sections were embedded in molten paraffin wax. The initial steps of spermiogenesis were depicted by changes in acrosomic granules, whereas further stages were identified by nuclear morphological changes. Ten steps of spermiogenesis and nine stages of seminiferous epithelium were identified. Four types of spermatogonia Ad, Ap1, Ap2 and B were recognized. The results depicted a clear variation in the diameter of seminiferous tubules (ST), epithelium height (EH), luminal tubular diameter (LD), number of seminiferous tubules per field and the Johnsen score. Microscopy indicated that the stages of seminiferous epithelium were similar to other birds and mammals and the ST diameter, EH, LD and Johnsen score are positively correlated while the number of seminiferous tubules per field is negatively correlated with the ST diameter, EH, LD and Johnsen score. Fertility in Yangzhou ganders can further be improved by visualizing the histological development of germs cells in testis tissues during spermatogenesis after onset of breeding season and maturity. Our results suggest that Yangzhou ganders reach complete sexual maturity at 227 days of age.

scholarly journals Pathogenic effect of bunt wheat on male reproductive system in Mices.

2010 ◽  
Vol 34 (2) ◽  
pp. 9-14
Author(s):  
Mustafa A.K. Al-Taie
Keyword(s):  
Experimental Study ◽  
Basement Membrane ◽  
Sertoli Cells ◽  
Reproductive System ◽  
Seminiferous Tubule ◽  
Male Fertility ◽  
Normal Diet ◽  
Male Reproductive System ◽  
The Other ◽  
Histopathological Study

This study was carried out to study the toxic effect of smut wheat on male reproductive system of mice. The experimental study was performed in the laboratory using 20 mice, which divided into two groups, one group feed on normal diet, the other fed on a mixture of 50% normal diet with 50% smut wheat. A histopathological study was done on the testes of Mice fed on smut wheat for 30 days. The histopathological study revealed thickness of basement membrane of semiriferous tubule of mice, and desquamation of the sertoli cells and spermatogonium from the basement membrane of seminiferous tubule and has adversely effect on male fertility.

High-fructose diet during puberty alters the sperm parameters, testosterone concentration, and histopathology of testes and epididymis in adult Wistar rats

2021 ◽  
pp. 1-8
Author(s):  
Daniele Sapede Alvarenga Medaglia ◽  
Henrique Rodrigues Vieira ◽  
Sandra da Silva Silveira ◽  
Gláucia Eloisa Munhoz de L. Siervo ◽  
Monique Suellen da Silva Marcon ◽  
...  
Keyword(s):  
Reproductive System ◽  
Wistar Rats ◽  
Metabolic Diseases ◽  
Serum Testosterone ◽  
Seminiferous Tubules ◽  
Adult Rats ◽  
High Fructose Diet ◽  
Fructose Intake ◽  
High Fructose ◽  
Male Wistar Rats

Abstract The consumption of fructose has increased in children and adolescents and is partially responsible for the high incidence of metabolic diseases. The lifestyle during postnatal development can result in altered metabolic programming, thereby impairing the reproductive system and fertility during adulthood. Therefore, the aim of this study was to evaluate the effect of a high-fructose diet in the male reproductive system of pubertal and adult rats. Male Wistar rats (30 d old) were assigned to four different groups: Fr30, which received fructose (20%) in water for 30 d and were euthanized at postnatal day (PND) 60; Re-Fr30, which received fructose (20%) for 30 d and were euthanized at PND 120; and two control groups C30 and Re-C30, which received water ad libitum and were euthanized at PND 60 and 120, respectively. Fructose induced an increase in abnormal seminiferous tubules with epithelial vacuoles, degeneration, and immature cells in the lumen. Moreover, Fr30 rats showed altered spermatogenesis and daily sperm production (DSP), as well as increased serum testosterone concentrations. After discontinuing high-fructose consumption, DSP and sperm number decreased significantly. We observed tissue remodeling in the epididymis, with a reduction in stromal and epithelial compartments that might have influenced sperm motility. Therefore, we concluded that fructose intake in peripubertal rats led to changes in the reproductive system observed both during puberty and adulthood.

Spermatogenesis recovery in the mouse after iron injury

2003 ◽  
Vol 22 (5) ◽  
pp. 275-279 ◽  
Author(s):  
Maria De Lourdes Pereira ◽  
Fernando Garciae Costa
Keyword(s):  
Sertoli Cells ◽  
Morphological Changes ◽  
Recovery Period ◽  
Seminiferous Epithelium ◽  
Seminiferous Tubules ◽  
Prosthetic Devices ◽  
Transmission Electron ◽  
Normal Functional ◽  
Iron Nickel ◽  
Cytological Features

Alloys used as prosthetic devices for bone/joint replacement include some heavy metals such as chromium, iron, nickel, or titanium. Unfortunately, due to the aggressive nature of the physiological environment, corrosion of these alloys promotes the release of metal ions into the surrounding tissues causing systemic toxic effects. Our previous preliminary studies have demonstrated that iron induced several morphological changes within mice seminiferous epithelium. The aim of the present work was to investigate, over a one-month period, the possibility of recovery of mice seminiferous epithelium, previously damaged by iron. Male Charles River mice were dosed subcutaneously with 0.5 mL of an iron suspension of 538 mg/L9-10 10 mg/L (n-5) every 72 hours during two weeks, followed by a recovery period of 30 days. Fragments of the seminiferous tubules were fixed in glutaraldehyde and prepared for light and transmission electron microscopy. Regeneration of spermatogenesis was noted after a one-month period, as illustrated by the presence of normal germ cells, in the usual position within the seminiferous tubules. These germinal elements and the Sertoli cells have shown normal cytological features. These results strongly suggest that the deleterious effects induced by iron are reversible. The presence of residual bodies within Sertoli cells cytoplasm indicates that they are able to perform a normal functional activity in a recovered spermatogenesis.

scholarly journals Effects of Diabetes Mellitus on the Reproductive System of Adult Male Mice After One Cycle of Spermatogenesis

2021 ◽  
Vol 17 (34) ◽  
pp. 149
Author(s):  
Kouassi Emile Bégbin ◽  
N’Guessan Ernest Zougrou ◽  
Georges Abizi ◽  
Koffi Kouakou ◽  
N’Takpé Emmanuel Jaurès Mangué
Keyword(s):  
Diabetes Mellitus ◽  
Adult Male ◽  
Reproductive System ◽  
Male Reproductive System ◽  
Diabetic Group ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Male Mice ◽  
Chronic Hyperglycemia ◽  
Study Results

Background: Diabetes mellitus is a disease characterized by chronic hyperglycemia. Poor management of hyperglycemia leads to complications that can reduce quality of life. Diabetes mellitus has been associated with damage to the reproductive system. The present study examines effects of diabetes mellitus on the reproductive system in alloxan-induced diabetic mice after one cycle of spermatogenesis. Materials and Methods: Twelve (12) mice divided into "control" and "diabetic" groups of six (6) animals each were used. Diabetes was induced in adult male mice by intraperitoneal injection with a single dose of 220 mg/kg body weight of alloxan. After 40 days, sperm density, morphology, and motility were assessed by standard methods. Serum levels of testosterone, FSH, and LH were measured. In addition, testes and epididymis were removed for histological study. Results: Highly significant (P < 0.001) reductions in serum testosterone, FSH, and LH levels, as well as normal and motile sperm counts of 21% and 34% respectively were observed in the diabetic group. The control group had three times more sperm than the diabetic group. The histological increased inter-tubular spaces, loss of interstitial tissue, degeneration of seminiferous tubules, and almost complete reduction of sperm count in the epididymal duct. Conclusion: Chronic hyperglycemia is therefore deleterious to the male reproductive system of mice. It leads to hypogonadism, which causes dysfunction of the male reproductive system, and can lead to infertility in men with diabetes mellitus.

scholarly journals Spermatogenic and Histologic Evaluation of Imiprothrin in Male Reproductive System of Adult Wistar Rats

Pharmacologia ◽  
2017 ◽  
Vol 8 (2) ◽  
pp. 52-58
Author(s):  
Ojewale Abdulfatai Olakunle ◽  
Akingbade Adebanji Modupe ◽  
Onyekuru Gift Uzochukwu ◽  
Olasehinde Oluwaseun Ruth ◽  
Ibitoye babatunde oluwaseun ◽  
...  
Keyword(s):  
Reproductive System ◽  
Wistar Rats ◽  
Male Reproductive System ◽  
Histologic Evaluation
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