scholarly journals Spatial and temporal anoxia in single-osculum Halichondria panicea demosponge explants studied with planar optodes

2021 ◽  
Vol 168 (12) ◽  
Author(s):  
Lars Kumala ◽  
Morten Larsen ◽  
Ronnie N. Glud ◽  
Donald E. Canfield
Keyword(s):  
Time Lapse ◽  
Rate Of Change ◽  
Halichondria Panicea ◽  
Lifetime Imaging ◽  
Flow Through ◽  
Contraction And Expansion ◽  
Oxygen Dynamics ◽  
Insight Into ◽  
Aquiferous System ◽  
Observation Period

AbstractThe water flow through sponges is regulated by their contractile behaviour including contraction and expansion of the aquiferous system, which leads to shifting oxygen levels in the sponge interior. Still, knowledge of spatial and temporal anoxia in sponges is lacking, but important in elucidating interactions between sponge hosts and their microbiomes. We combined 2-D luminescence lifetime imaging of oxygen with simultaneous time-lapse recordings of the sponge exhalant opening (osculum) to unveil temporal as well as spatial oxygen dynamics caused by contractile behaviour in single-osculum explants of the demosponge Halichondria panicea. The present study reveals an intrinsic concentric deoxygenation pattern in explants during episodes of osculum contraction generating an oxygen gradient with increasing concentrations towards the explant periphery. Four sponge explants faced 25 episodes with substantial changes in internal oxygen and anoxia which prevailed for 4.4 h of the total 92.0 h observation period. The 2-D images revealed that the total area of the explant experiencing anoxia during periods of osculum contraction–expansion varied between 0.01 and 13.22% and was on average 7.4 ± 4.4% for all sponge explants. Furthermore, oxygen respiration, as approximated by the rate of change of oxygen concentration during deoxygenation of the explant interior, was similar throughout the oxic parts of the explant base. The resolved 2-D dynamics provide an unprecedented insight into the internal O2 distribution of sponges and complement the traditional point measurements of oxygen sensors.

Copper–oxygen Dynamics in Tyrosinase

2019 ◽  
Author(s):  
Nobutaka Fujieda ◽  
Sachiko Yanagisawa ◽  
Minoru Kubo ◽  
Genji Kurisu ◽  
Shinobu Itoh
Keyword(s):  
Catalytic Mechanism ◽  
Substrate Binding ◽  
Active Form ◽  
Copper Ion ◽  
Atomic Resolution ◽  
Oxygen Species ◽  
X Ray ◽  
Oxygen Dynamics ◽  
Insight Into ◽  
Copper Centre

To unveil the activation of dioxygen on the copper centre (Cu<sub>2</sub>O<sub>2</sub>core) of tyrosinase, we performed X-ray crystallograpy with active-form tyrosinase at near atomic resolution. This study provided a novel insight into the catalytic mechanism of the tyrosinase, including the rearrangement of copper-oxygen species as well as the intramolecular migration of copper ion induced by substrate-binding.<br>

scholarly journals HARBOR STUDY FOR SAN NICOLAS BAY, PERU

1970 ◽  
Vol 1 (12) ◽  
pp. 133
Author(s):  
James M. Keith ◽  
Emmett J. Murphy
Keyword(s):  
Particle Velocity ◽  
Time Lapse ◽  
Wave Action ◽  
Ship Motion ◽  
Long Period ◽  
Wave Property ◽  
Directional Wave ◽  
Detailed Interpretation ◽  
Wave Phenomena ◽  
Insight Into

A pair of ducted impeller current meters, one mounted vertically and the other horizontally, were used to measure wave action at San Nicolas Harbor, Peru The horizontal water velocity records are superior to conventional wave records because they measure directly the wave property which induces adverse horizontal ship motion, and provide directional wave data Spectral analysis methods proved well-suited to detailed interpretation of the particle velocity records, while considerable insight into the wave phenomena was gained by simple, rational inspections and interpretations of the records Time-lapse movies of a moored ship, when correlated with simultaneous water particle velocity records, provided an exceptionally clear picture of ship response to wave action, and led to the rather surprising observation that long-period ship motion is not necessarily caused by long-period waves The foregoing ship response was duplicated in hydrau1ic model tests.

scholarly journals A family of T6SS antibacterial effectors related to L,D-transpeptidases targets the Peptidoglycan

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Stephanie Sibinelli de Sousa ◽  
Julia Takuno Hespanhol ◽  
Bruno Matsuyama ◽  
Stephane Mesnage ◽  
Gianlucca Nicastro ◽  
...  
Keyword(s):  
Cell Envelope ◽  
Point Mutations ◽  
Time Lapse ◽  
Secretion Systems ◽  
Bacterial Antagonism ◽  
New Family ◽  
Type Vi Secretion ◽  
Altered Cell ◽  
Bacterial Effectors ◽  
Insight Into

Type VI secretion systems (T6SSs) are contractile nanomachines widely used by bacteria to intoxicate competitors. Salmonella Typhimurium encodes a T6SS within the Salmonella pathogenicity island 6 (SPI-6) that is used during competition against species of the gut microbiota. We characterized a new SPI-6 T6SS antibacterial effector named Tlde1 (type VI L,D-transpeptidase effector 1). Tlde1 is toxic in target-cell periplasm and its toxicity is neutralized by co-expression with immunity protein Tldi1 (type VI L,D-transpeptidase immunity 1). Time-lapse microscopy revealed that intoxicated cells display altered cell division and lose cell envelope integrity. Bioinformatics analysis showed that Tlde1 is evolutionarily related to L,D-transpeptidases. Point mutations on conserved histidine121 and cysteine131 residues eliminated toxicity. Co-incubation of purified recombinant Tlde1 and peptidoglycan tetrapeptides showed that Tlde1 displays both L,D-carboxypeptidase activity by cleaving GM-tetrapeptides between meso-diaminopimelic acid3 and D-alanine4, and L,D-transpeptidase exchange activity by replacing D-alanine4 for a non-canonical D-amino acid. Tlde1 constitutes a new family of T6SS effectors widespread in Proteobacteria. This work increases our knowledge about the bacterial effectors used in interbacterial competitions and provides molecular insight into a new mechanism of bacterial antagonism.

Inertia-Driven Controlled Passive Actuation

2015 ◽  
Author(s):  
Douwe Dresscher ◽  
Theo J. A. de Vries ◽  
Stefano Stramigioli
Keyword(s):  
Energy Flow ◽  
Legged Locomotion ◽  
Continuously Variable Transmission ◽  
The State ◽  
Rate Of Change ◽  
Storage Element ◽  
Abstraction Layer ◽  
Variable Transmission ◽  
Alternative Means ◽  
Flow Through

A serious problem with using electrical actuators in legged locomotion is the significant energy loss. For this reason, we propose and analyse an alternative means of actuation: Controlled Passive Actuation. Controlled Passive Actuation aims at reducing the energy flow through electric actuators by actuating with a combination of an energy storage element and a Continuously Variable Transmission. In this work, we present a method where we apply a Continuously Variable Transmission with a storage element in the form of a mass to change the state of another mass (“the load”). An abstraction layer is created to abstract the inertia-driven Controlled Passive Actuation to a source of effort, a force actuator. On this abstracted system, feedback control can be applied to achieve control goals such as path tracking. With simulations and experiments, we show that inertia-driven Controlled Passive Actuation can be used to control the state of an (inertial) load. The experimental results show that the performance of the system is affected by the internal dynamics and limited rate of change of the transmission ratio of the Continuously Variable Transmission.

Cell behaviour in a polygonal cell sheet*

Development ◽  
1984 ◽  
Vol 83 (Supplement) ◽  
pp. 313-327
Author(s):  
H. Honda ◽  
R. Kodama ◽  
T. Takeuchi ◽  
H. Yamanaka ◽  
K. Watanabe ◽  
...  
Keyword(s):  
Cell Sheet ◽  
Cell Monolayer ◽  
Time Lapse ◽  
Geometrical Method ◽  
Cell Behaviour ◽  
Cell Monolayers ◽  
Polygonal Cell ◽  
Computer Based ◽  
Insight Into

Cell monolayers on culture dishes were divided into two groups: tensile monolayers and non-tensile ones. In the development of an epithelium, a non-tensile cell monolayer turns into a tightly bound tensile one. Detection of these states was carried out by using the boundary shortening procedure, a computer-based geometrical method to show how much the polygonal cell boundary contracts. Non-tensile monolayers were divided further into two groups according to their motility: a fluctuating monolayer in which cells move laterally, and a stable monolayer in which cells are immobilized. Quantitative determination of cell motility was performed by analysing time-lapse cellular patterns. These computer-based geometrical analyses enabled us to divide monolayers into three groups: tensile stable monolayers, non-tensile stable monolayers and fluctuating monolayers, and this study therefore gives an insight into the way in which changing conformations of cells may be assayed.

scholarly journals Impact of Bed Form Celerity on Oxygen Dynamics in the Hyporheic Zone

Water ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 62 ◽  
Author(s):  
Philipp Wolke ◽  
Yoni Teitelbaum ◽  
Chao Deng ◽  
Jörg Lewandowski ◽  
Shai Arnon
Keyword(s):  
Hyporheic Zone ◽  
Time Lapse ◽  
Oxygen Distribution ◽  
Anoxic Zone ◽  
Fine Grain ◽  
Uptake Rates ◽  
Hyporheic Zones ◽  
Bed Form ◽  
Gradual Disappearance ◽  
Oxygen Dynamics

Oxygen distribution and uptake in the hyporheic zone regulate various redox-sensitive reactions and influence habitat conditions. Despite the fact that fine-grain sediments in streams and rivers are commonly in motion, most studies on biogeochemistry have focused on stagnant sediments. In order to evaluate the effect of bed form celerity on oxygen dynamics and uptake in sandy beds, we conducted experiments in a recirculating indoor flume. Oxygen distribution in the bed was measured under various celerities using 2D planar optodes. Bed morphodynamics were measured by a surface elevation sensor and time-lapse photography. Oxygenated zones in stationary beds had a conchoidal shape due to influx through the stoss side of the bed form, and upwelling anoxic water at the lee side. Increasing bed celerity resulted in the gradual disappearance of the upwelling anoxic zone and flattening of the interface between the oxic (moving fraction of the bed) and the anoxic zone (stationary fraction of the bed), as well as in a reduction of the volumetric oxygen uptake rates due shortened residence times in the hyporheic zone. These results suggest that including processes related to bed form migration are important for understanding the biogeochemistry of hyporheic zones.

scholarly journals THEORETICAL ANALYSIS OF THE LEAKAGE THROUGH THE CEMENT LINE OF A SINGLE OSTEON

2020 ◽  
Vol 20 (02) ◽  
pp. 1950073
Author(s):  
GAFFAR GAILANI ◽  
STEPHEN COWIN
Keyword(s):  
Theoretical Analysis ◽  
Pore Pressure ◽  
Previous Analysis ◽  
Outer Boundary ◽  
Theoretical Solution ◽  
Porous Structures ◽  
Cement Line ◽  
A Value ◽  
Flow Through ◽  
Insight Into

This work focuses on the Lacunar–Canalicular Porosity (PLC) of cortical bone which includes the osteons. Osteons are semicylindrical porous structures saturated with fluid within the bone and are approximately 250[Formula: see text][Formula: see text]m in diameter. The outer boundary of the osteon is called the cement line. Some studies suggested that the cement line is less highly mineralized and produced evidence that it has less calcium and phosphorus and more sulfur than the neighboring bone lamellae. Most authors assume that the cement line is impermeable, while others assume that some canaliculi are crossing the cement line which will make it permeable to certain degree. The objective of this work is to develop a theoretical analysis to study the leakage through the cement line and its relationship with the pore pressure distribution. The theoretical analysis is developed using our previous analysis for osteon under harmonic loading with addition of leakage parameter. The leakage parameter varies from 0 to 1, where a value of 0 indicates free flow through the cement line and a value of 1 indicates no flow through the cement line. Experimental results could be compared to this developed theoretical solution to get in depth understanding of the effect of leakage on osteon poroelastic properties. Additionally, the developed theoretical solution will give insight into sensitivity of osteon pore pressure to leakage through the cement line.

scholarly journals Plasmid segregation: spatial awareness at the molecular level

2007 ◽  
Vol 179 (5) ◽  
pp. 813-815 ◽  
Author(s):  
Jakob Møller-Jensen ◽  
Kenn Gerdes
Keyword(s):  
Molecular Level ◽  
Time Lapse ◽  
Spatial Awareness ◽  
Division Plane ◽  
Plasmid Partition ◽  
Plasmid Segregation ◽  
Low Copy Number ◽  
Coli Plasmid ◽  
Cell Division Plane ◽  
Insight Into

In bacteria, low-copy number plasmids ensure their stable inheritance by partition loci (par), which actively distribute plasmid replicates to each side of the cell division plane. Using time-lapse fluorescence microscopic tracking of segregating plasmid molecules, a new study provides novel insight into the workings of the par system from Escherichia coli plasmid R1. Despite its relative simplicity, the plasmid partition spindle shares characteristics with the mitotic machinery of eukaryotic cells.

CFD simulation of core annular flow through sudden contraction and expansion

2012 ◽  
Vol 86-87 ◽  
pp. 153-164 ◽  
Author(s):  
V.V.R. Kaushik ◽  
Sumana Ghosh ◽  
Gargi Das ◽  
Prasanta Kumar Das
Keyword(s):  
Annular Flow ◽  
Cfd Simulation ◽  
Sudden Contraction ◽  
Flow Through ◽  
Contraction And Expansion

scholarly journals Turbogenerator Steam Turbine Variation in Developed Power: Analysis of Exergy Efficiency and Exergy Destruction Change

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Vedran Mrzljak ◽  
Tomislav Senčić ◽  
Božica Žarković
Keyword(s):  
Steam Turbine ◽  
Mass Flow ◽  
Power Analysis ◽  
Exergy Efficiency ◽  
Operating Point ◽  
Exergy Destruction ◽  
Flow Through ◽  
Power Measurements ◽  
Operating Points ◽  
Insight Into

Developed power variation of turbogenerator (TG) steam turbine, which operates at the conventional LNG carrier, allows insight into the change in turbine exergy efficiency and exergy destruction during the increase in turbine power. Measurements of required operating parameters were performed in eight different TG steam turbine operating points during exploitation. Turbine exergy efficiency increases from turbine power of 500 kW up to 2700 kW, and maximum exergy efficiency was obtained at 70.13% of maximum turbine developed power (at 2700 kW) in each operating point. From turbine developed power of 2700 kW until the maximum power of 3850 kW, exergy efficiency decreases. Obtained change in TG turbine exergy efficiency is caused by an uneven intensity of increase in turbine developed power and steam mass flow through the turbine. TG steam turbine exergy destruction change is directly proportional to turbine load and to steam mass flow through the turbine—higher steam mass flow results in a higher turbine load which leads to the higher exergy destruction and vice versa. The higher share of turbine developed power and the lower share of turbine exergy destruction in the TG turbine exergy power inlet lead to higher turbine exergy efficiencies. At each observed operating point, turbine exergy efficiency in exploitation is lower when compared to the maximum obtained one for 8.39% to 12.03%.

Sign in / Sign up

Export Citation Format

Share Document