scholarly journals Triterpenoid gene expression and phytochemical content in Iranian licorice under salinity stress

PROTOPLASMA ◽  
2019 ◽  
Vol 256 (3) ◽  
pp. 827-837 ◽  
Author(s):  
Zahra Shirazi ◽  
Ali Aalami ◽  
Masoud Tohidfar ◽  
Mohammad Mehdi Sohani
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Rasmita Rani Das ◽  
Seema Pradhan ◽  
Ajay Parida

AbstractScreening the transcriptome of drought tolerant variety of little millet (Panicum sumatrense), a marginally cultivated, nutritionally rich, susbsistent crop, can identify genes responsible for its hardiness and enable identification of new sources of genetic variation which can be used for crop improvement. RNA-Seq generated ~ 230 million reads from control and treated tissues, which were assembled into 86,614 unigenes. In silico differential gene expression analysis created an overview of patterns of gene expression during exposure to drought and salt stress. Separate gene expression profiles for leaf and root tissue revealed the differences in regulatory mechanisms operating in these tissues during exposure to abiotic stress. Several transcription factors were identified and studied for differential expression. 61 differentially expressed genes were found to be common to both tissues under drought and salinity stress and were further validated using qRT-PCR. Transcriptome of P. sumatrense was also used to mine for genic SSR markers relevant to abiotic stress tolerance. This study is first report on a detailed analysis of molecular mechanisms of drought and salinity stress tolerance in a little millet variety. Resources generated in this study can be used as potential candidates for further characterization and to improve abiotic stress tolerance in food crops.


2020 ◽  
Vol 18 ◽  
pp. 100423
Author(s):  
Chao Li ◽  
Na Li ◽  
Tiantian Dong ◽  
Qiang Fu ◽  
Yanting Cui ◽  
...  

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2477
Author(s):  
Rehab El-Dakak ◽  
Weam El-Aggan ◽  
Ghadah Badr ◽  
Amira Helaly ◽  
Amel Tammam

Strategic implementation of vermicompost as safe biofertilizer besides defensing saline soils offer dual function solving problems in developing countries. The current study aims to utilize vermicompost (VC) for amelioration of 200mM NaCl in Vicia faba Aspani cultivar and investigate the molecular role of salt overly sensitive pathway (SOS1). The experiment was conducted following a completely randomized design with three replicates. Treatments include 0; 2.5; 5; 10; 15% dried VC intermingled with soil mixture (clay: sand; 1:2) and/or 200 mM NaCl. The results show that salinity stress decreased broad bean fresh and dry weight; and K+/Na+. However, malonedialdehyde and H2O2 contents; increased. Application of 10% VC and salinity stress increases Ca2+ (41% and 50%), K+/Na+ (125% and 89%), Mg2+ (25% and 36%), N (8% and 11%), indole acetic acid (70% and 152%) and proteins (9% and 13%) for root and shoot, respectively, in comparison to salt treated pots. Moreover, all examined enzymatic antioxidants and their substrates increased, except glutathione reductase. A parallel decrease in abscisic acid (75% and 29%) and proline (59% and 58%) was also recorded for roots and leaves, respectively. Interestingly, the highly significant increase in gene expression of SOS1 (45-fold) could drive defense machinery of broad bean to counteract 200 mM NaCl.


2020 ◽  
Vol 19 (4) ◽  
pp. 21-30
Author(s):  
Nastaran Rashidi ◽  
Ramezan Ali Khavari-Nejad ◽  
Parvin Ramak ◽  
Sara Saadatmand

Sweet basil is an important medicinal plant belonging to Lamiaceae family. In this plant, Phenylpropanoid pathway possesses some enzymes involving in generating suitable essential oil constituents. The main purpose of conducting this study was to investigate the effects of chitosan on sweet basil’s growth and physiological parameters as well as gene expression subjected to salinity stress. After employing a foliar-spray of chitosan at 0 (as control) and 0.2 gl–1, the plants were subjected to salinity treatments at 0, 25, 50, 100, and 150 mM NaCl. The results of this research revealed that chitosan, compared to the controls, improved growth parameters under stressed or non-stressed conditions. In this regard, chitosan increased protein and chlorophyll contents as well as the expression of PAL and COVMT genes leading to an increase in phenolic compounds. To sum up, chitosan improved sweet basil tolerance to salinity through influencing the genes involved in the pathway of phenylpropanoid so as to produce secondary metabolites.


2021 ◽  
Author(s):  
Tofigh Mazalmazraei ◽  
Leila nejadsadeghi ◽  
Khosro Mehdikhanlou ◽  
Daryoosh Nabati Ahmadi

Abstract Sugarcane is an important industrial plant which cultivated in the most arid and semiarid regions. Due to climate change and anthropogenic activities, the sugarcane field damage due to salt deposition and the cultivation of sugarcane has been posed a major threat in the region. To address this issue, the identification of salinity tolerant cultivars would be a suitable strategy to minimize yield loss in the area. MicroRNAs (miRNAs) play important roles in regulating gene expression. The monitoring of the expression of miRNAs and their targeted genes could provide deeper insight into the molecular stress mechanism and screen tolerant cultivars. Our aim was to assess the expression of nine candidate miRNAs and their corresponding targeted genes among the studied sugarcane cultivars under salinity condition, leading to identify the salt-tolerant cultivar. To achieve our goal, a two-factorial experiment with three sugarcane cultivars (CP-48, CP-57, CP-69) and two salinity levels (0 and 8 ds/m) was conducted. One-way ANOVA indicated that there was a significant difference between miRNAs and targeted gene expression. The highest reduction of miRNAs expression was occurred in miR160 while the lower one was happening in miR1432. The data also indicated that the higher and the lowest of targeted genes were in miR160 and miR393 respectively. Among studied cultivars, the CP-57 showed poor performance while CP-69 expresses a superior tolerance to salt stress. Taken together, these results suggested that the screening of well adapted cultivars under salt conditions would be appropriate solutions to combat salinity stress in saline lands.


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