Expression and Purification of the Main Component Contained in Camel Milk and Its Antimicrobial Activities Against Bacterial Plant Pathogens

2018 ◽  
Vol 10 (4) ◽  
pp. 787-793 ◽  
Author(s):  
Abbas Tanhaeian ◽  
Farajollah Shahriari Ahmadi ◽  
Mohammad Hadi Sekhavati ◽  
Mojtaba Mamarabadi
Keyword(s):  
Plant Pathogens ◽  
Antimicrobial Activities ◽  
Camel Milk ◽  
Expression And Purification ◽  
Main Component

scholarly journals Synergistic Antimicrobial Activities of Combinations of Vanillin and Essential Oils of Cinnamon Bark, Cinnamon Leaves and Cloves

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1406
Author(s):  
Rita Cava-Roda ◽  
Amaury Taboada-Rodríguez ◽  
Antonio López-Gómez ◽  
Ginés Benito Martínez-Hernández ◽  
Fulgencio Marín-Iniesta
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Food Packaging ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Inhibitory Effects ◽  
Cinnamon Bark ◽  
E Coli ◽  
Pure Compounds ◽  
Main Component

Plant bioactive compounds have antimicrobial and antioxidant activities that allow them to be used as a substitute for synthetic chemical additives in both food and food packaging. To improve its sensory and bactericidal effects, its use in the form of effective combinations has emerged as an interesting possibility in the food industry. In this study, the antimicrobial activities of essential oils (EOs) of cinnamon bark, cinnamon leaves, and clove and the pure compounds vanillin, eugenol, and cinnamaldehyde were investigated individually and in combination against Listeria monocytogenes and Escherichia coli O157:H7. The possible interactions of combinations of pure compounds and EOs were performed by the two-dimensional checkerboard assay and isobologram methods. Vanillin exhibited the lowest antimicrobial activity (MIC of 3002 ppm against L. monocytogenes and 2795 ppm against E. coli O157:H7), while clove and cinnamon bark EOs exhibited the highest antimicrobial activity (402–404 against L. monocytogenes and 778–721 against E. coli O157:H7). For L. monocytogenes, pure compound eugenol, the main component of cinnamon leaves and clove, showed lower antimicrobial activity than EOs, which was attributed to the influence of the minor components of the EOs. The same was observed with cinnamaldehyde, the main component of cinnamon bark EO. The combinations of vanillin/clove EO and vanillin/cinnamon bark EO showed the most synergistic antimicrobial effect. The combination of the EOs of cinnamon bark/clove and cinnamon bark/cinnamon leaves showed additive effect against L. monocytogenes but indifferent effect against E. coli O157:H7. For L. monocytogenes, the best inhibitory effects were achieved by cinnamon bark EO (85 ppm)/vanillin (910 ppm) and clove EO (121 ppm)/vanillin (691 ppm) combinations. For E. coli, the inhibitory effects of clove EO (104 ppm)/vanillin (1006 ppm) and cinnamon leaves EO (118 ppm)/vanillin (979 ppm) combinations were noteworthy. Some of the tested combinations increased the antimicrobial effect and would allow the effective doses to be reduced, thereby offering possible new applications for food and active food packaging.

Extracts and metabolites derived from the leaves of Cassia alata L. exhibit in vitro and in vivo antimicrobial activities against fungal and bacterial plant pathogens

2021 ◽  
Vol 166 ◽  
pp. 113465
Author(s):  
Duong Quang Pham ◽  
Hieu Trung Pham ◽  
Jae Woo Han ◽  
Tung Huu Nguyen ◽  
Huong Thanh Nguyen ◽  
...  
Keyword(s):  
Plant Pathogens ◽  
Antimicrobial Activities ◽  
Cassia Alata

scholarly journals Antifungal activity in vitro of Sapranthus microcarpus (Annonaceae) against phytopathogens

2018 ◽  
Author(s):  
Iván De-la-Cruz-Chacón ◽  
Nora Yazmin López-Fernández ◽  
Christian Anabí Riley-Saldaña ◽  
Marisol Castro Moreno ◽  
Alma Rosa González-Esquinca
Keyword(s):  
Antifungal Activity ◽  
Fungal Pathogens ◽  
Plant Pathogens ◽  
Stem Bark ◽  
Antimicrobial Activities ◽  
Curvularia Lunata ◽  
Root Bark ◽  
Inhibitory Activities ◽  
Alkaloid Extracts

Background and Aims: The phytochemistry study of Annonaceae has intensified in the last decades due to the discovery of secondary metabolites with antimicrobial activities. The inhibitory activities of extracts and compounds from these species for phytopathogens are less known. The antifungal activity of Sapranthus microcarpus was determined in vitro against six fungal pathogens of important crops.Methods: Hexane, methanol, and alkaloid extracts of the leaves and root and stem bark of S. microcarpus were evaluated to determine whether they could inhibit the mycelial growth of the phytopathogens. The combined activity of the most active extracts for each phytopathogen was also determined. The alkaloid liriodenine was isolated and identified as an antifungal principle.Key results: All extracts inhibited the growth of the six phytopathogens to some extent. The root bark alkaloid extract showed the highest activity. Fusarium oxysporum f. sp. lycopersici and Curvularia lunata were the most sensitive phytopathogens. Liriodenine was active against all the plant pathogens (minimum inhibitory concentrations of 125-500 nmol ml-1).Conclusions: Sapranthus microcarpus is a native natural resource with great phytochemical potential.

scholarly journals Azadirachta indica Reduces Black Sigatoka in East African Highland Banana by Direct Antimicrobial Effects against Mycosphaerella fijiensis without Inducing Resistance

2017 ◽  
Vol 9 (4) ◽  
pp. 61 ◽  
Author(s):  
Alfred Kumakech ◽  
Hans. J. L. Jørgensen ◽  
David B. Collinge ◽  
Richard Edema ◽  
Patrick Okori
Keyword(s):  
Plant Extracts ◽  
Azadirachta Indica ◽  
Plant Pathogens ◽  
Antimicrobial Activities ◽  
Late Time ◽  
Black Sigatoka ◽  
East African ◽  
Pr Genes ◽  
Antimicrobial Effects ◽  
And Control

Black Sigatoka is a major disease of East African highland cooking bananas in Uganda. Aqueous extracts of Azadirachta indica, Cinnamomum zeylanicum and Capsicum annuum have shown the potential to reduce Black Sigatoka in banana plantlets. The mechanisms by which plant extracts confer protection against plant pathogens has previously been reported to involve activation of defence and direct antimicrobial activity. In the current study, both antimicrobial activities of selected extracts were studied as well as expression of three defence-related genes using quantitative real-time PCR. Gene expression was compared in susceptible (cv. Musakala, genomic group AAA-EA) and resistant (cv. Kayinja, genomic group ABB) banana cultivars. Additionally, Musakala treated with A indica extract at 1 day before inoculation (DBI) was tested for induction of defence-related genes at 0, 10 and 20 days after inoculation (DAI). Pathogenesis-related genes (PR-1 and PR-3) and non-expressor of PR-genes (NPR1B) were up-regulated in the resistant cultivar. The genes analysed responded at late time points to M. fijiensis inoculation in both extract-treated and control plants in the susceptible cv. Musakala. On the other hand, A. indica and C. annuum completely inhibited mycelial growth of M. fijiensis at 30% (w/v). These findings suggest that the effect of plant extracts on Black Sigatoka is strongly associated with the direct antimicrobial effects.

scholarly journals Comparative Analysis of Antimicrobial Activities of Valinomycin and Cereulide, the Bacillus cereus Emetic Toxin

2011 ◽  
Vol 77 (8) ◽  
pp. 2755-2762 ◽  
Author(s):  
Marcel H. Tempelaars ◽  
Susana Rodrigues ◽  
Tjakko Abee
Keyword(s):  
Antimicrobial Activity ◽  
Comparative Analysis ◽  
Membrane Potential ◽  
Bacillus Cereus ◽  
Antimicrobial Activities ◽  
Gram Positive ◽  
Content Type ◽  
Gram Positive Bacteria ◽  
Ph Range ◽  
Main Component

ABSTRACTCereulide and valinomycin are highly similar cyclic dodecadepsipeptides with potassium ionophoric properties. Cereulide, produced by members of theBacillus cereusgroup, is known mostly as emetic toxin, and no ecological function has been assigned. A comparative analysis of the antimicrobial activity of valinomycin produced byStreptomycesspp. and cereulide was performed at a pH range of pH 5.5 to pH 9.5, under anaerobic and aerobic conditions. Both compounds display pH-dependent activity against selected Gram-positive bacteria, includingStaphylococcus aureus,Listeria innocua,Listeria monocytogenes,Bacillus subtilis, andBacillus cereusATCC 10987. Notably,B. cereusstrain ATCC 14579 and the emeticB. cereusstrains F4810/72 and A529 showed reduced sensitivity to both compounds, with the latter two strains displaying full resistance to cereulide. Both compounds showed no activity against the selected Gram-negative bacteria. Antimicrobial activity against Gram-positive bacteria was highest at alkaline pH values, where the membrane potential (ΔΨ) is the main component of the proton motive force (PMF). Furthermore, inhibition of growth was observed in both aerobic and anaerobic conditions. Determination of the ΔΨ, using the membrane potential probe DiOC2(3) (in the presence of 50 mM KCl) in combination with flow cytometry, demonstrated for the first time the ability of cereulide to dissipate the ΔΨ in sensitive Gram-positive bacteria. The putative role of cereulide production in the ecology of emeticB. cereusis discussed.

scholarly journals Facilitation of Expression and Purification of an Antimicrobial Peptide by Fusion with Baculoviral Polyhedrin in Escherichia coli

2005 ◽  
Vol 71 (9) ◽  
pp. 5038-5043 ◽  
Author(s):  
Quande Wei ◽  
Young Soo Kim ◽  
Jeong Hyun Seo ◽  
Woong Sik Jang ◽  
In Hee Lee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fusion Protein ◽  
High Performance ◽  
Affinity Purification ◽  
Antimicrobial Activities ◽  
Host Cells ◽  
Functional Study ◽  
Fusion Partner ◽  
Expression And Purification ◽  
E Coli

ABSTRACT Several fusion strategies have been developed for the expression and purification of small antimicrobial peptides (AMPs) in recombinant bacterial expression systems. However, some of these efforts have been limited by product toxicity to host cells, product proteolysis, low expression levels, poor recovery yields, and sometimes an absence of posttranslational modifications required for biological activity. For the present work, we investigated the use of the baculoviral polyhedrin (Polh) protein as a novel fusion partner for the production of a model AMP (halocidin 18-amino-acid subunit; Hal18) in Escherichia coli. The useful solubility properties of Polh as a fusion partner facilitated the expression of the Polh-Hal18 fusion protein (∼33.6 kDa) by forming insoluble inclusion bodies in E. coli which could easily be purified by inclusion body isolation and affinity purification using the fused hexahistidine tag. The recombinant Hal18 AMP (∼2 kDa) could then be cleaved with hydroxylamine from the fusion protein and easily recovered by simple dialysis and centrifugation. This was facilitated by the fact that Polh was soluble during the alkaline cleavage reaction but became insoluble during dialysis at a neutral pH. Reverse-phase high-performance liquid chromatography was used to further purify the separated recombinant Hal18, giving a final yield of 30% with >90% purity. Importantly, recombinant and synthetic Hal18 peptides showed nearly identical antimicrobial activities against E. coli and Staphylococcus aureus, which were used as representative gram-negative and gram-positive bacteria, respectively. These results demonstrate that baculoviral Polh can provide an efficient and facile platform for the production or functional study of target AMPs.

Antioxidant and antimicrobial activities of four Astragalus species growing wild in Turkey

2018 ◽  
Vol 43 (4) ◽  
pp. 425-434 ◽  
Author(s):  
Sevil Albayrak ◽  
Onur Kaya
Keyword(s):  
Radical Scavenging ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Breast Cancer Cell Lines ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Phenolic Contents ◽  
Agar Well Diffusion Assay ◽  
Main Component ◽  
Mcf 7

Abstract Objective The objective of the present study was to investigate the phenolic compositions, antioxidant, antimicrobial and cytotoxic activities of four Astragalus species, two of which are endemic to Turkish flora. Methods The total phenolic and flavonoid contents of methanol extracts obtained from four Astragalus species were detected using Folin-Ciocalteu and aluminum chloride colorimetric assays. Their phenolic compositions were identified by Liquid chromatography–mass spectrometry (LC-MS). The antioxidant activity was assayed with phosphomolybdenum, 2,2-diphenyl-1-picrylhydrazyl radical scavenging (DPPH), hydrogen peroxide scavenging, β-carotene bleaching activity, ferric-ion reducing power (FRAP) and cupric ions (Cu2+) reducing antioxidant capacity (CUPRAC) methods. Antimicrobial activities of the extracts were studied by agar well-diffusion assay. The cytotoxic effects of the extracts on MCF-7 (human breast cancer cell lines) were determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) method. Results The extracts exerted moderate antioxidant and reducing activity with low phenolic contents. The main component in the extracts was determined as ferulic acid. The extracts demonstrated no antibacterial activity except P. aeruginosa. A. talasseus showed the highest cytotoxic activity on MCF-7 during 48 h. Conclusion It is believed that the results of this study will contribute to research recently increasing for the use of natural antioxidant and antimicrobial compounds in many industrial fields such as food, pharmacy and medicine.

scholarly journals Comparative Efficacy of Aloe vera (Linn) and Aloe schweinfurthii (Baker) Powdered Leaf Extracts in the Control of Some Plant Fungal Pathogens

2019 ◽  
pp. 1-9
Author(s):  
A. O. Alejo ◽  
A. M. Ajayi ◽  
B. O. Akinyele
Keyword(s):  
Fungal Pathogens ◽  
Plant Pathogens ◽  
Cold Water ◽  
Aloe Vera ◽  
Water Extract ◽  
Antimicrobial Activities ◽  
Hot Water ◽  
Colletotrichum Lindemuthianum ◽  
Leaf Extracts ◽  
Hot Water Extract

Evaluation of the relative efficacy of powdered leaf extracts of Aloe vera (Linn) and Aloe schweinfurthii (Baker) in the control of some plant pathogens was undertaken in this work. Antimicrobial activities of the extracts obtained using cold water, hot water and ethanol were tested against four fungal spp., namely, Alternaria solani, Colletotrichum lindemuthianum, Sclerotium rolfsii and Trichophyton rubrum. The phytochemical screening of the leaf extracts of the two aloe species revealed the presence of bioactive compounds such as alkaloids, tannins, saponins, flavonoids, cardiac glycosides, phytates and oxalates. The extracts were observed to exhibit varying inhibitory effects on the selected fungi. Ethanolic extract of A. vera at 50 mg/ml and 100 mg/ml had the greatest impact on A. solani and C. lindemuthianum respectively. Similarly, cold water extract of A. schweinfurthii at 100 mg/ml was the most effective against S. rolfsii and T. rubrum. However, hot water extract of A. vera was least effective against C. lindemuthianum. Also, the efficacy of cold water extract of A. schweinfurthii at 50 mg/ml was very low against T. rubrum and A. solani. The hot water extract of A. schweinfurthii at 20 mg/ml also showed the least effect against S. rolfsii. Consequently, extracts from both Aloe species can be recommended in the management of the four fungal pathogens evaluated in this study. It is hoped that in no distant future, botanical fungicides would be developed from the two Aloe species.

scholarly journals Complete Genome Sequence Resource for the Endophytic Burkholderia sp. Strain MS389 Isolated from a Healthy Soybean Growing Adjacent to Charcoal Rot Disease Patch

Plant Disease ◽  
2021 ◽  
Author(s):  
Jiayuan Jia ◽  
Emerald Ford ◽  
Sonya M. Baird ◽  
Shien Lu
Keyword(s):  
Fungal Pathogen ◽  
Plant Pathogens ◽  
Endophytic Bacterium ◽  
Macrophomina Phaseolina ◽  
Antimicrobial Activities ◽  
Soybean Plant ◽  
Charcoal Rot ◽  
Protein Coding ◽  
Rot Disease ◽  
Circular Chromosomes

Burkholderia sp. strain MS389, an endophytic bacterium, was isolated from a healthy soybean plant growing adjacent to a patch of plants affected by charcoal rot disease, caused by the fungal pathogen Macrophomina phaseolina. Preliminary studies demonstrated that strain MS389 possesses antimicrobial activities against multiple plant pathogens. Burkholderia sp. strain MS389 was found to have three circular chromosomes of 3,563,380 bp, 3,002,449bp, and 1,180,421 bp in size, respectively. The 7,746,250-bp genome, with 66.73% G+C content, harbors 6,756 protein coding genes in the predicted 6,985 genes. In total, 18 rRNAs, 68 tRNAs, 4 ncRNAs were identified and 139 pseudogenes were annotated as well. The findings of this study will provide valuable data to explore the antimicrobial mechanisms of the endophytic bacterial strain.

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