scholarly journals Synthesis and characterization of ZnO nanoparticles using aqueous extract of Becium grandiflorum for antimicrobial activity and adsorption of methylene blue

2021 ◽  
Vol 11 (2) ◽  
Author(s):  
Mebrahtu Hagos Kahsay
Keyword(s):  
Antimicrobial Activity ◽  
Plant Extract ◽  
Klebsiella Pneumonia ◽  
Zno Nps ◽  
Synthesis Of Nanoparticles ◽  
Synthesis Strategy ◽  
Vis Spectroscopy ◽  
Methods Of Synthesis ◽  
Aqueous Leaf Extract ◽  
Xrd Patterns

AbstractNanotechnology is a recent field of modern research dealing with synthesis, strategy and manipulation of particle’s structure in size range of 1–100 nm. This study introduces one of the methods of synthesis of nanoparticles, i.e., green synthesis of ZnO NPs using aqueous leaf extract of Becium grandiflorum (AM: ‘Yedegamentisie’). The biomolecules of the plant extract (such as phenols, flavonoids, saponins, glycosides, steroids, tannins and alkaloids) were used as capping and reducing agent during synthesis of ZnO NPs. Response surface methodology coupled with Box-Behnken design (RSM-BBD) was used to optimize the synthesis of ZnO NPs and adsorption studies of the as-synthesized ZnO NPs. Then, ZnO NPs was characterized using different spectroscopic and microscopic instruments such as UV–Vis spectroscopy, FTIR, XRD and SEM–EDS to consider its purity, shape and crystallinity. UV–Vis analysis showed peaks in the range 305–312 nm due to synthesis of ZnO NPs. FTIR analysis showed the availability of different phytochemicals in the plant extract and synthesis of ZnO NPs at 490 cm−1. Powder XRD patterns confirmed formation of phase pure wurtzite structures of ZnO NPs. The synthesized ZnO NPs were used to remove MB dye from aqueous solution by acting as a photocatalyst and adsorbent as well as, it also showed antimicrobial activity against two gram positive (Staphylococcus epidermidis, Staphylococcus aureus) and three gram negative (Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa) bacteria.

Green Synthesis of Silver Nanoparticles by Allamanda cathartica L. Leaf Extract and Evaluation for Antimicrobial Activity

2013 ◽  
Vol 6 (4) ◽  
pp. 2260-2268
Author(s):  
M. Linga Rao ◽  
Bhumi G ◽  
Savithramma N
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Fusarium Species ◽  
Inhibition Zone ◽  
Fungal Species ◽  
Green Technology ◽  
Metallic Silver ◽  
Synthesis Of Nanoparticles ◽  
Aqueous Leaf Extract

Silver nanoparticles (SNPs) exhibit tremendous applications in medicine as antimicrobial agent.  The use of different parts of plants for the synthesis of nanoparticles is considered as a green technology as it does not involve any harmful chemicals.  In the present study, we report a rapid biosynthesis of silver nanoparticles from aqueous leaf extract of medicinal plant Allamanda cathartica.  The active phytochemicals present in the plant were responsible for the quick reduction of silver ion to metallic silver nanoparticles. The reduced silver nanoparticles were characterized by using UV-Vis spectrophotometry, Scanning Electron Microscope (SEM), Energy Dispersive Analysis of X-ray (EDAX) and Atomic Force Microscopy (AFM).  The spherical shaped silver nanoparticles were observed and it was found to 19-40 nm range of size.  These phytosynthesized SNPs were tested for their antimicrobial activity and it analyzed by measuring the inhibitory zone. A. cathartica aqueous leaf extract of SNPs showed highest toxicity to Pseudomonas followed by Klebsiella, Bacillus and E. coli and lowest toxicity towards Proteus. In fungal species, highest inhibition zone was noted against Rhizopus followed by Curvularia, Aspergillus flavus and Aspergillus niger and minimum inhibition zone was observed against Fusarium species.  These results suggest a promising potential of Indian plant-based green chemistry for production of SNPs for biomedical and nanotechnology applications.

scholarly journals Biosynthesis of Gold Nanoparticles Using Fusarium oxysporum f. sp. cubense JT1, a Plant Pathogenic Fungus

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Janki N. Thakker ◽  
Pranay Dalwadi ◽  
Pinakin C. Dhandhukia
Keyword(s):  
Antimicrobial Activity ◽  
Gold Nanoparticles ◽  
Chloride Solution ◽  
Pathogenic Fungus ◽  
Particle Size Analysis ◽  
Size Analysis ◽  
Plant Pathogenic Fungus ◽  
Extracellular Synthesis ◽  
Vis Spectroscopy ◽  
Methods Of Synthesis

The development of reliable processes for the synthesis of gold nanoparticles is an important aspect of current nanotechnology research. Recently, reports are published on the extracellular as well as intracellular biosynthesis of gold nanoparticles using microorganisms. However, these methods of synthesis are rather slow. In present study, rapid and extracellular synthesis of gold nanoparticles using a plant pathogenic fungus F. oxysporum f. sp. cubense JT1 (FocJT1) is reported. Incubation of FocJT1 mycelium with auric chloride solution produces gold nanoparticles in 60 min. Gold nanoparticles were characterized by UV-Vis spectroscopy, FTIR, and particle size analysis. The particles synthesized were of 22 nm sized, capped by proteins, and posed antimicrobial activity against Pseudomonas sp.

scholarly journals BIOMIMETIC SYNTHESIS, CHARACTERIZATION AND EVALUATION OF ANTIOXIDANT, ANTIMICROBIAL EFFICACY OF SILVER NANOPARTICLES USING ANDREDERA CORDIFOLIA LEAF EXTRACT

2017 ◽  
Vol 10 (4) ◽  
pp. 329 ◽  
Author(s):  
Rajathi Palanivelrajan ◽  
Suja S
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Biomimetic Synthesis ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Frap Assay ◽  
Vis Spectroscopy

Objective: The present study is focussed on the biosynthesis of silver nanoparticles using aqueous extract of Andredera cordifolia and to investigate the free radical scavenging potential, antimicrobial activity of the nanoparticles against different human pathogens.Methods: The formation of silver nanoparticles was indicated by the colour change from colourless to reddish brown. Biosynthesized nanoparticles was characterised using several techniques, viz- UV-Vis spectroscopy, FT-IR, XRD, TEM, SEM and EDAX analysis. The free radical scavenging potential was measured by DPPH, FRAP assay, antimicrobial activity against six microorganisms was tested using disc diffusion method.  Results: UV-Vis spectral analysis showed silver Surface Plasmon Resonance band at 426 nm.  The crystalline morphology and size of the nanoparticles were determined by TEM, SEM, and X-ray diffraction studies which showed the average size of the nanoparticles in the range 40 –60nm.  The biologically synthesized nanoparticles efficiently inhibited pathogenic organisms such as E. coli, Staphylococcus aureus, Klebsiella Pneumonia, Pseudomonas aeruginosa and Proteus vulgaris.  The biosynthesized nanoparticles might serve as a potent antioxidant as revealed by DPPH assay and FRAP assay. Conclusion: The biosynthesis of silver nanoparticles had several advantages in pharmaceutical applications as well as large scale commercial production.Key Words: Silver nanoparticle, Andredera cordifolia leaf, SEM,TEM,XRD,antioxidant, antimicrobial,etc.

scholarly journals BIOMIMETIC SYNTHESIS AND ANTIBACTERIAL PROPERTIES OF STRONTIUM OXIDE NANOPARTICLES USING OCIMUM SANCTUM LEAF EXTRACT

2018 ◽  
Vol 11 (3) ◽  
pp. 384 ◽  
Author(s):  
Apsana G ◽  
George Pp ◽  
Devanna N ◽  
Yuvasravana R
Keyword(s):  
Leaf Extract ◽  
Antibacterial Properties ◽  
Biomimetic Synthesis ◽  
Ocimum Sanctum ◽  
Klebsiella Pneumonia ◽  
Oxide Nanoparticles ◽  
Strontium Nitrate ◽  
Strontium Oxide ◽  
Synthesis Of Nanoparticles ◽  
Vis Spectroscopy

 Objective: The investigation and synthesis of nanoparticles using green chemical methods is an emerging field due to ecologically derived materials. In the present study, the reaction under microwave irradiation technique is proposed for synthesizing the strontium oxide nanoparticles (SrO NPs) by reacting the strontium nitrate powder with Ocimum sanctum L. leaf extract followed by heat treatment at 500oC for 2 h.Methods: The crystalline nature, size, and morphological structure of the SrO powder sample were characterized by techniques such as powder X-ray diffraction (PXRD), scanning electron microscopy, ultraviolet (UV)-Vis spectroscopy, Fourier-transform infrared spectroscopy (FTIR) and photoluminescence (PL) spectral analysis. To investigate the antibacterial properties of SrO NPs, pathogens such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella pneumonia, and Morganella morganii was tested in Mueller–Hinton disc diffusion method.Results: The particle size of the SrO NPs is calculated to be 42 nm using Debye–Scherrer equation. The SrO NPs exhibited absorption peak in the range of 250–350 nm centered at 274 nm in UV region. In addition, it is demonstrated that band gap energy was calculated to be 5.39 eV from Tauc’s plot. The SrO NPs has shown the efficient antibacterial effect by inhibiting zone against Gram-negative bacteria.Conclusion: The main aim of the study is to synthesize cost-effective and eco-friendly green synthesis of SrO NPs from the OS (O. sanctum) leaf extract and their characterization. The mechanism for the formation of SrO NPs in the presence of eugenol as reducing agent is also discussed. The present green chemical approach using plant-based materials for the synthesis of nanoparticles enhances the eco-friendliness, compatibility, effectiveness, and reduces the toxicity.

scholarly journals Synthesis and Characterization of Green Zinc Oxide Nanoparticles with Antiproliferative Effects through Apoptosis Induction and MicroRNA Modulation in Breast Cancer Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-17
Author(s):  
Amir Hossein Aalami ◽  
Mohammad Mesgari ◽  
Amirhossein Sahebkar
Keyword(s):  
Breast Cancer ◽  
Antimicrobial Activity ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Fluorescent Dyes ◽  
Cytotoxicity Test ◽  
Mcf7 Cells ◽  
Zno Nps ◽  
Vis Spectroscopy ◽  
The Impact

Changes in the expression of microRNAs can affect cancer cells’ viability and behavior and the impact on cancer treatment. In this study, the expression of miR-155-5p, miR-203a-3p, and miR-223-3p in the MCF7 cancer cell line was studied when exposed to ZnO nanoparticles synthesized through a green route. Mentioned ZnO-NPs were well characterized by UV-vis spectroscopy, DLS, XRD, FTIR, FE-SEM, EDX, zeta potential, and AFM analyses. Cellular studies were conducted using ZnO-NPs before miRNA investigations including MTT cytotoxicity test against MCF7, MDA-MB-231, and HFF cell lines. Moreover, apoptosis assays were performed using morphological analysis, fluorescent dyes, flow cytometry, and evaluation of caspase-3 and caspase-8 gene expression. Biological properties such as the antioxidant and antimicrobial activity of these novel ZnO-NPs were considered. MTT assays showed that the inhibitory concentration (IC50) of ZnO-NPs after 24 h was 11.16 μg/mL, 60.08 μg/mL, and 26.3 μg/mL on MCF7, MDA-MB-231, and HFF cells, respectively. The qRT-PCR results showed reduced expression of miR-155-5p, miR-203a-3p, and miR-223-3p when the MCF7 cells were treated with the IC50 concentration of ZnO-NPs (11.16 μg/mL). The antioxidant activity results showed EC50 values at 57.19 μg/mL and 31.5 μg/mL in DPPH and ABTS assays, respectively. The antimicrobial activity of ZnO-NPs was determined on Gram-negative and Gram-positive bacterial strains and fungi using MIC and MBC assays. These NPs had a significant effect in reducing the expression of microRNAs in breast cancer cells. Finally, ZnO-NPs exerted antioxidant and antimicrobial activities.

scholarly journals Efficient synthesis of plant-mediated silver nanoparticles and their screening for antimicrobial activity

2017 ◽  
Vol 4 (3) ◽  
pp. 143-150 ◽  
Author(s):  
Kamlesh Shukla ◽  
Bhoopander Giri ◽  
Rashmi Dwivede
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Silver Nitrate ◽  
Color Change ◽  
Bacterial Species ◽  
Nitrate Solution ◽  
Reducing Agent ◽  
Curvularia Lunata ◽  
Synthesis Of Nanoparticles ◽  
Vis Spectroscopy

Now days, the development of safe, cost effective, reliable and eco-friendly processes for the synthesis of nanoparticles is an important aspect of nanotechnology. Among the various agents, plants show immense potential for the synthesis of nanoparticles. The bio-molecules found in plants induce reduction of Ag+ ions from silver nitrate to silver nanoparticles (AgNPs); therefore, in the present work, the aqueous leaves extract of the plant was used as reducing agent for the synthesis of silver nanoparticles. We synthesized extracellular silver nanoparticles using extract of the leaves of four different medicinal plants which act as a reducing agent at room temperature. The characteristic color change was observed on addition of plant extract to the silver nitrate solution due to their specific properties (Surface Plasmon Resonance). UV-Vis spectroscopy was used for the characterization of the silver nanoparticles. Green synthesized nanoparticles are evaluated for their antimicrobial activity against the Gram-positive and Gram-negative bacteria as well as two pathogenic fungi Aspergillus fumigatus and Curvularia lunata. The silver nanoparticles (SNPs) of selected plant parts have shown more toxicity towards bacterial species than that of the fungal species. Comparing with simple plant extracts, the SNPs exhibited greater antimicrobial efficacy and advantage over conventional antibiotics to which these microorganisms usually impart resistance.

scholarly journals Antimicrobial Activity of Ca-Alginate/Chitosan Nanocomposite Loaded with Camptothecin

Polymers ◽  
2021 ◽  
Vol 13 (20) ◽  
pp. 3559
Author(s):  
Wafa Al-Gethami ◽  
Noha Al-Qasmi
Keyword(s):  
Antimicrobial Activity ◽  
Calcium Alginate ◽  
Chemical Interaction ◽  
Genetic Material ◽  
Point Mutations ◽  
Polymerase Chain Reaction Analysis ◽  
Pcr Analysis ◽  
Klebsiella Pneumonia ◽  
E Coli ◽  
Vis Spectroscopy

The main aim of this study was to prepare antimicrobial nanocomposites consisting of alginate, chitosan, and camptothecin (CPT). CPT-loaded calcium alginate (Ca-Alg2) and calcium alginate/chitosan (Ca-Alg2-CH) nanomaterials were synthesized and characterized using infrared (IR) spectroscopy, X-ray diffraction (XRD), UV-Vis spectroscopy, and scanning electron microscopy (SEM). The antimicrobial activity and the genetic effects of Ca-Alg2/CPT and Ca-Alg2-CH/CPT nanomaterials on Staphylococcus aureus, Escherichia coli, and Klebsiella pneumonia were studied. The repetitive element polymerase chain reaction analysis technique was used to assess the changes in the bacterial genetic material due to the processing of the nanomaterials. The results showed the presence of a strong chemical interaction between alginate and chitosan, and CPT was loaded successfully in both Ca-Alg2/CPT and Ca-Alg2-CH/CPT nanomaterials. Furthermore, the antimicrobial test showed that the Ca-Alg2/CPT nanocomposite was susceptible to S. aureus, E. coli, and K. pneumonia; on the other hand, Ca-Alg2-CH/CPT nanocomposite was more susceptible to E. coli and K. pneumonia and was resistant to S. aureus. The results showed that the Ca-Alg2/CPT nanocomposite was less efficient than Ca-Alg2-CH/CPT nanocomposite in killing Gram-negative treated bacteria. Moreover, results revealed that the PCR analysis revealed a polymorphic banding pattern. This observation provides an excellent guide to the ability of some polymers to induce point mutations in DNA.

scholarly journals Optical and Structural Investigation of Mg2+ doped ZnO Nanoparticles using Gymnema sylvestre and Mangifera indica Leaves Extracts

2019 ◽  
Vol 5 (1) ◽  
pp. 607-609
Author(s):  
M. Karthikeyan . ◽  
A. Jafar Ahamed . ◽  
P. Vijaya Kumar .
Keyword(s):  
Zno Nanoparticles ◽  
Structural Investigation ◽  
Mangifera Indica ◽  
Gymnema Sylvestre ◽  
X Ray Diffraction ◽  
Zno Nps ◽  
Synthesis Of Nanoparticles ◽  
Vis Spectroscopy ◽  
Doped Zno ◽  
Physical And Chemical

In green nanotechnology, plant is used for the synthesis of nanoparticles which are gaining considerable interest among researchers as an eco-friendly alternative to conventional physical and chemical methods, as this approach eliminates the use of toxic chemicals. The present study describes the synthesis of Mg2+ (magnesium) doped zinc oxide (ZnO) nanoparticles (NPs) M1 using leaves extract of Gymnema sylvestre (G. sylvestre) belonging to Asclepiadaceae family and M2 using Mangifera indica (M. indica) belonging to Anacardiaceae family as reducing as well as capping agents. The obtained Mg2+ doped ZnO NPs (M1 and M2) were characterized by X-ray diffraction (XRD) studies, field emission scanning electron microscopy (FESEM), elemental analysis (EDAX), Fourier transform infrared spectroscopy (FTIR), UV-vis spectroscopy and photoluminescence (PL) spectra.

scholarly journals Green Synthesis of Silver Nanoparticles from several NTFP Plants

2016 ◽  
Vol 8 (1) ◽  
pp. 106-111
Author(s):  
Somnath BHOWMIK ◽  
Badal Kumar DATTA ◽  
Ajay Krishna SAHA ◽  
Pradyut CHAKMA ◽  
Narayan Chandra MANDAL
Keyword(s):  
Silver Nanoparticles ◽  
Plant Extract ◽  
Silver Ions ◽  
Biological Synthesis ◽  
Synthesis Of Nanoparticles ◽  
Force Microscopy ◽  
Atomic Force ◽  
One Step ◽  
Aqueous Leaf Extract ◽  
Uv Visible

The biological synthesis of nanoparticles using plant extracts plays an important role in the field of nanotechnology. In this study, rapid, simple approach was applied for synthesis of silver nanoparticles using , Clerodendrum infortunatum, Mucuna interrupta, Phlogancanthus thyrsiflorus and Sansevieria trifasciata aqueous leaf extract. The plant extract acts both as reducing agent as well as capping agent. To identify the compounds responsible for reduction of silver ions, the functional groups present in plant extract were investigated by FTIR. Various techniques used to characterize synthesized nanoparticles are Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM) and UV–Visible spectrophotometer. Results confirmed that this protocol was simple, rapid, one step, eco-friendly, non-toxic and might be an alternative conventional physical/chemical methods. Conversion of silver nanoparticles takes place at room temperature without the involvement of any hazardous chemicals.

scholarly journals Callus-mediated biosynthesis of Ag and ZnO nanoparticles using aqueous callus extract of Cannabis sativa: Their cytotoxic potential and clinical potential against human pathogenic bacteria and fungi

2021 ◽  
Vol 10 (1) ◽  
pp. 569-584
Author(s):  
Mehreen Zaka ◽  
Syed Salman Hashmi ◽  
Moiz A. Siddiqui ◽  
Lubna Rahman ◽  
Sadaf Mushtaq ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Cannabis Sativa ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
Ag Nps ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
Antibacterial And Antifungal Activities ◽  
Vis Spectroscopy ◽  
Bacteria And Fungi

Abstract In this paper, we have presented the method of green synthesis of ZnO and Ag-NPs using the callus extract (CE) of medicinally important Cannabis sativa. The synthesis of nanoparticles (NPs) was confirmed by UV-Vis spectroscopy, while as far as the size and shape of the NPs were concerned, they were validated using the techniques of X-ray diffraction and scanning electron microscopy, respectively. The energy dispersive X-ray analysis graph confirmed the constitution of elements along with the surface chemical state of NPs. Fourier transform-infrared spectroscopy was utilized for the confirmation of biomolecules capping the NPs. In order to test the application of these biosynthesized NPs on biological entities, four bacterial strains, including Bacillus subtilis, Klebsiella pneumonia, Staphylococcus aureus, and Pseudomonas aeruginosa, were used. On the other hand, five fungal strains, namely Mucor, Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, and Fusarium solani, were utilized for antifungal assay. Cytotoxicity assay was also performed using the HepG2 cell line. The results showed considerable antibacterial and antifungal activities. It also showed better cytotoxicity values as compared to the control.

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