Decreased biofilm formation by planktonic cells of Listeria monocytogenes in the presence of sodium hypochlorite

2021 ◽  
Vol 96 ◽  
pp. 103714
Author(s):  
Mohit Bansal ◽  
Nitin Dhowlaghar ◽  
Ramakrishna Nannapaneni ◽  
Divya Kode ◽  
Sam Chang ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Sodium Hypochlorite ◽  
Biofilm Formation ◽  
Planktonic Cells

Chlorine Resistance of Listeria monocytogenes Biofilms and Relationship to Subtype, Cell Density, and Planktonic Cell Chlorine Resistance

2006 ◽  
Vol 69 (6) ◽  
pp. 1292-1296 ◽  
Author(s):  
JAMES P. FOLSOM ◽  
JOSEPH F. FRANK
Keyword(s):  
Stainless Steel ◽  
Listeria Monocytogenes ◽  
Cell Density ◽  
Sodium Hypochlorite ◽  
Resistance Mechanisms ◽  
Planktonic Cell ◽  
Planktonic Cells ◽  
Planktonic Culture

Strains of Listeria monocytogenes vary in their ability to produce biofilms. This research determined if cell density, planktonic chlorine resistance, or subtype are associated with the resistance of L. monocytogenes biofilms to chlorine. Thirteen strains of L. monocytogenes were selected for this research based on biofilm accumulation on stainless steel and rep-PCR subtyping. These strains were challenged with chlorine to determine the resistance of individual strains of L. monocytogenes. Planktonic cells were exposed to 20 to 80 ppm sodium hypochlorite in 20 ppm increments for 5 min in triplicate per replication, and the experiment was replicated three times. The number of tubes with surviving L. monocytogenes was recorded for each isolate at each level of chlorine. Biofilms of each strain were grown on stainless steel coupons. The biofilms were exposed 60 ppm of sodium hypochlorite. When in planktonic culture, four strains were able to survive exposure to 40 ppm of chlorine, whereas four strains were able to survive 80 ppm of chlorine in at least one of three tubes. The remaining five strains survived exposure to 60 ppm of chlorine. Biofilms of 11 strains survived exposure to 60 ppm of chlorine. No association of biofilm chlorine resistance and planktonic chlorine resistance was observed; however, biofilm chorine resistance was similar for strains of the same subtype. Biofilm cell density was not associated with chlorine resistance. In addition, biofilms that survived chlorine treatment exhibited different biofilm morphologies. These data suggest that chlorine resistance mechanisms of planktonic cells and biofilms differ, with planktonic chlorine resistance being more affected by inducible traits, and biofilm chlorine resistance being more affected by traits not determined in this study.

scholarly journals Inhibition of Biofilm Formation and Related Gene Expression of Listeria monocytogenes in Response to Four Natural Antimicrobial Compounds and Sodium Hypochlorite

2021 ◽  
Vol 11 ◽  
Author(s):  
Yunge Liu ◽  
Lina Wu ◽  
Jina Han ◽  
Pengcheng Dong ◽  
Xin Luo ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Quorum Sensing ◽  
Sodium Hypochlorite ◽  
Biofilm Formation ◽  
Natural Compounds ◽  
Antimicrobial Compounds ◽  
Natural Antimicrobial ◽  
Low Degree ◽  
Chemical Disinfectant ◽  
Dose Dependent

The aim of this study was to assess the efficacy of four natural antimicrobial compounds (cinnamaldehyde, eugenol, resveratrol and thymoquinone) plus a control chemical disinfectant (sodium hypochlorite) in inhibiting biofilm formation by Listeria monocytogenes CMCC54004 (Lm 54004) at a minimum inhibitory concentration (MIC) and sub-MICs. Crystal violet staining assay and microscopic examination were employed to investigate anti-biofilm effects of the evaluated compounds, and a real-time PCR assay was used to investigate the expression of critical genes by Lm 54004 biofilm. The results showed that five antimicrobial compounds inhibited Lm 54004 biofilm formation in a dose dependent way. Specifically, cinnamaldehyde and resveratrol showed better anti-biofilm effects at 1/4 × MIC, while sodium hypochlorite exhibited the lowest inhibitory rates. A swimming assay confirmed that natural compounds at sub-MICs suppressed Lm 54004 motility to a low degree. Supporting these findings, expression analysis showed that all four natural compounds at 1/4 × MIC significantly down-regulated quorum sensing genes (agrA, agrC, and agrD) rather than suppressing the motility- and flagella-associated genes (degU, motB, and flaA). This study revealed that sub-MICs of natural antimicrobial compounds reduced biofilm formation by suppressing the quorum sensing system rather than by inhibiting flagella formation.

Biofilm Formation and Biocides Sensitivity of Pseudomonas marginalis Isolated from a Maple Sap Collection System

2006 ◽  
Vol 69 (10) ◽  
pp. 2411-2416 ◽  
Author(s):  
L. LAGACÉ ◽  
M. JACQUES ◽  
A. A. MAFU ◽  
D. ROY
Keyword(s):  
Sodium Hypochlorite ◽  
Peracetic Acid ◽  
Biofilm Formation ◽  
Planktonic Cells ◽  
Collection System ◽  
Biofilm Growth ◽  
Biofilm Production ◽  
Different Temperatures ◽  
Maple Sap ◽  
Scanning Electron

The susceptibility of planktonic and biofilm cells of Pseudomonas marginalis toward four commonly used biocides at different temperatures (15 and 30°C) and biofilm growth times (24 and 48 h) was assessed. Using the MBEC biofilm device, biofilm production in maple sap was shown to be highly reproducible for each set of conditions tested. Biofilm formation was influenced by growth temperature and time. A temperature of 15°C and incubation time of 24 h yielded fewer CFU per peg and showed fewer adhered cells and typical biofilm structures, based on scanning electron microscopy observations as compared with other conditions. Minimal biofilm eradication concentration values for P. marginalis were significantly greater (P < 0.001) than were MBCs for planktonic cells and for every biocide tested, with the exception of minimal biofilm eradication concentration values for peracetic acid at 15°C and 24 h. Sodium hypochlorite and peracetic acid sanitizers were able to eliminate P. marginalis biofilms at lower concentrations as compared with hydrogen peroxide– and quaternary ammonium– based sanitizers (P < 0.001). According to the results obtained, sodium hypochlorite and peracetic acid sanitizers would be more appropriate for maple sap collection system sanitation.

Eradication of Pseudomonas biofilm by disinfectants and some plants extracts

2015 ◽  
Vol 5 (1) ◽  
pp. 06-16
Author(s):  
Lattab Aicha ◽  
Belkacem Imane ◽  
Djibaoui Rachid ◽  
Rebai Oifa ◽  
Chibani Abdelwaheb ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Sodium Hypochlorite ◽  
Biofilm Formation ◽  
Culture Medium ◽  
Allium Sativum ◽  
Reference Strain ◽  
Aloe Vera ◽  
Planktonic Cells ◽  
Lawsonia Inermis ◽  
Two Cultures

In the present study three isolates belonging to Pseudomonas sp and one reference strain of P. aeruginosa ATCC 27853 were tested for biofilm formation on two types of support (glass and polystyrene), using two cultures medium Tryptone Soy Broth (TSB) and Modified Biofilm Broth (MBB). The results showed that the quantity of biofilm formed depends on the nature of culture medium, where the rate of the adherent bacteria was more significant in TSB medium. Polystyrene was more favorable to bacteria for adherence compared to glass. We examined the effectiveness of three types of disinfectants, sodium hypochlorite, hydrogen peroxide and temperature on a biofim formed by the studied bacteria. Sodium hypochlorite reached good levels of biofilm eradication using all isolates adhered on the two types of support. Hydrogen peroxide exerted less significant effect compared to sodium hypochlorite, eliminating approximately 56% from the biofilm adhered on polystyrene at concentration of 3%. The elimination of biofilm temperature (80°C) was rather weak compared with the two chemical disinfectants. Our study included the testing of extracts of three plants: Allium sativum, Aloe vera and Lawsonia inermis on biofilm eradication formed by P. aeruginosa ATCC 27853. The effect of these plant extracts on planktonic cells was also studied. The results showed that Allium sativum and Lawsonia inermis inhibit both bacterial growth and biofilm formation and no activity was detected for Aloe vera extract.

scholarly journals Effect of different conditions on Listeria monocytogenes biofilm formation and removal

2018 ◽  
Vol 36 (No. 3) ◽  
pp. 208-214 ◽  
Author(s):  
Pasquale Russo ◽  
Agni Hadjilouka ◽  
Luciano Beneduce ◽  
Vittorio Capozzi ◽  
Spiros Paramithiotis ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Listeria Monocytogenes ◽  
Sodium Hypochlorite ◽  
Biofilm Formation ◽  
Benzalkonium Chloride ◽  
Food Products ◽  
Growth Media ◽  
Hydrophilic Surfaces ◽  
Different Temperatures ◽  
Process Line

Listeria monocytogenes poses a major risk for the safety of food products due to the ability to persist in food products and process line surfaces as biofilm. In this work, we investigated the L. monocytogenes biofilms in relation to development factors and possible control under different conditions. In particular, the ability of six strains of L. monocytogenes from vegetable and animal sources to form biofilms was evaluated on glass or polystyrene substrates under different temperatures (15, 30 and 37°C) and availability of nutrients, by using rich (BHI) or poor (HTM) growth media. Moreover, the effectiveness of three commonly used sanitizers (benzalkonium chloride, sodium hypochlorite and hydrogen peroxide) was compared to eradicate established biofilms. Our results showed that starved conditions, hydrophilic surfaces, and high temperatures increased the L. monocytogenes ability to produce biofilms. In general, benzalkonium chloride was the most effective chemical to remove established biofilms.

scholarly journals Proteomic Analysis of Listeria monocytogenes FBUNT During Biofilm Formation at 10°C in Response to Lactocin AL705

2021 ◽  
Vol 12 ◽  
Author(s):  
Constanza Melian ◽  
Patricia Castellano ◽  
Franco Segli ◽  
Lucía M. Mendoza ◽  
Graciela Margarita Vignolo
Keyword(s):  
Listeria Monocytogenes ◽  
Biofilm Formation ◽  
Acid Metabolism ◽  
Transport Systems ◽  
Sublethal Dose ◽  
Protein Abundance ◽  
Planktonic Cells ◽  
Adaptation Mechanisms ◽  
Specific Proteins ◽  
Sessile Cells

Listeria monocytogenes is one of the major food-related pathogens and is able to survive and multiply under different stress conditions. Its persistence in industrial premises and foods is partially due to its ability to form biofilm. Thus, as a natural strategy to overcome L. monocytogenes biofilm formation, the treatment with lactocin AL705 using a sublethal dose (20AU/ml) was explored. The effect of the presence of the bacteriocin on the biofilm formation at 10°C of L. monocytogenes FBUNT was evaluated for its proteome and compared to the proteomes of planktonic and sessile cells grown at 10°C in the absence of lactocin. Compared to planktonic cells, adaptation of sessile cells during cold stress involved protein abundance shifts associated with ribosomes function and biogenesis, cell membrane functionality, carbohydrate and amino acid metabolism, and transport. When sessile cells were treated with lactocin AL705, proteins’ up-regulation were mostly related to carbohydrate metabolism and nutrient transport in an attempt to compensate for impaired energy generation caused by bacteriocin interacting with the cytoplasmic membrane. Notably, transport systems such as β-glucosidase IIABC (lmo0027), cellobiose (lmo2763), and trehalose (lmo1255) specific PTS proteins were highly overexpressed. In addition, mannose (lmo0098), a specific PTS protein indicating the adaptive response of sessile cells to the bacteriocin, was downregulated as this PTS system acts as a class IIa bacteriocin receptor. A sublethal dose of lactocin AL705 was able to reduce the biofilm formation in L. monocytogenes FBUNT and this bacteriocin induced adaptation mechanisms in treated sessile cells. These results constitute valuable data related to specific proteins targeting the control of L. monocytogenes biofilm upon bacteriocin treatment.

Biofilm Formation and Associated Genes in Listeria Monocytogenes

2017 ◽  
Vol 12 (3) ◽  
Author(s):  
S. R. Warke ◽  
V. C. Ingle ◽  
N. V. Kurkure ◽  
P. A. Tembhurne ◽  
Minakshi Prasad ◽  
...  
Keyword(s):  
Public Health ◽  
Listeria Monocytogenes ◽  
Multiplex Pcr ◽  
Food Processing ◽  
Biofilm Formation ◽  
Milk Samples ◽  
Biofilm Production ◽  
Food Borne ◽  
Serious Infections ◽  
Microtiter Assay

Listeria monocytogenes, an opportunistic food borne pathogen can cause serious infections in immunocompromised individuals. L. monocytogenes is capable of producing biofilm on the surface of food processing lines and instruments.The biofilm transfers contamination to food products and impose risk to public health. In the present study biofilm producing ability of L. monocytogenes isolates were investigated phenotypically and genotypically by microtiter assay and multiplex PCR, respectively. Out of 38 L. monocytogenes isolates 14 were recovered from animal clinical cases, 12 bovine environment and 12 from milk samples. A total of 3 (21.42%) clinical, 2 (16.66%) environment and 3 (25%) milk samples respectively, revealed biofilm production in microtiter assay. Cumulative results showed that 23 (60.52%) out of 38 strains of L. monocytogenes were positive for luxS and flaA gene and 1 (2.63%) was positive only for the flaA gene.

scholarly journals In VitroAnalyses of the Effects of Heparin and Parabens on Candida albicans Biofilms and Planktonic Cells

2011 ◽  
Vol 56 (1) ◽  
pp. 148-153 ◽  
Author(s):  
Marisa H. Miceli ◽  
Stella M. Bernardo ◽  
T. S. Neil Ku ◽  
Carla Walraven ◽  
Samuel A. Lee
Keyword(s):  
Candida Albicans ◽  
Metabolic Activity ◽  
Biofilm Formation ◽  
High Dose ◽  
Dependent Manner ◽  
Planktonic Cells ◽  
Content Type ◽  
Heparin Sodium ◽  
The Individual

ABSTRACTInfections and thromboses are the most common complications associated with central venous catheters. Suggested strategies for prevention and management of these complications include the use of heparin-coated catheters, heparin locks, and antimicrobial lock therapy. However, the effects of heparin onCandida albicansbiofilms and planktonic cells have not been previously studied. Therefore, we sought to determine thein vitroeffect of a heparin sodium preparation (HP) on biofilms and planktonic cells ofC. albicans. Because HP contains two preservatives, methyl paraben (MP) and propyl paraben (PP), these compounds and heparin sodium without preservatives (Pure-H) were also tested individually. The metabolic activity of the mature biofilm after treatment was assessed using XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] reduction and microscopy. Pure-H, MP, and PP caused up to 75, 85, and 60% reductions of metabolic activity of the mature preformedC. albicansbiofilms, respectively. Maximal efficacy against the mature biofilm was observed with HP (up to 90%) compared to the individual compounds (P< 0.0001). Pure-H, MP, and PP each inhibitedC. albicansbiofilm formation up to 90%. A complete inhibition of biofilm formation was observed with HP at 5,000 U/ml and higher. When tested against planktonic cells, each compound inhibited growth in a dose-dependent manner. These data indicated that HP, MP, PP, and Pure-H havein vitroantifungal activity againstC. albicansmature biofilms, formation of biofilms, and planktonic cells. Investigation of high-dose heparin-based strategies (e.g., heparin locks) in combination with traditional antifungal agents for the treatment and/or prevention ofC. albicansbiofilms is warranted.

scholarly journals Advanced Killing Potential of Thymol against a Time and Temperature Optimized Attached Listeria monocytogenes Population in Lettuce Broth

Biomolecules ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 397
Author(s):  
Dimitra Kostoglou ◽  
Parthena Tsaklidou ◽  
Ioannis Iliadis ◽  
Nikoletta Garoufallidou ◽  
Georgia Skarmoutsou ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Benzalkonium Chloride ◽  
Planktonic Cells ◽  
Antimicrobial Efficiency ◽  
Fresh Vegetables ◽  
Viable Bacteria ◽  
Steel Surfaces ◽  
Polymerase Chain ◽  
Killing Action ◽  
Foodborne Infections

Fresh vegetables and salads are increasingly implicated in outbreaks of foodborne infections, such as those caused by Listeria monocytogenes, a dangerous pathogen that can attach to the surfaces of the equipment creating robust biofilms withstanding the killing action of disinfectants. In this study, the antimicrobial efficiency of a natural plant terpenoid (thymol) was evaluated against a sessile population of a multi-strain L. monocytogenes cocktail developed on stainless steel surfaces incubated in lettuce broth, under optimized time and temperature conditions (54 h at 30.6 °C) as those were determined following response surface modeling, and in comparison, to that of an industrial disinfectant (benzalkonium chloride). Prior to disinfection, the minimum bactericidal concentrations (MBCs) of each compound were determined against the planktonic cells of each strain. The results revealed the advanced killing potential of thymol, with a concentration of 625 ppm (= 4 × MBC) leading to almost undetectable viable bacteria (more than 4 logs reduction following a 15-min exposure). For the same degree of killing, benzalkonium chloride needed to be used at a concentration of at least 20 times more than its MBC (70 ppm). Discriminative repetitive sequence-based polymerase chain reaction (rep-PCR) also highlighted the strain variability in both biofilm formation and resistance. In sum, thymol was found to present an effective anti-listeria action under environmental conditions mimicking those encountered in the salad industry and deserves to be further explored to improve the safety of fresh produce.

scholarly journals Effect of Gaseous Ozone on Listeria monocytogenes Planktonic Cells and Biofilm: An In Vitro Study

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1484
Author(s):  
Felice Panebianco ◽  
Selene Rubiola ◽  
Francesco Chiesa ◽  
Tiziana Civera ◽  
Pierluigi Aldo Di Ciccio
Keyword(s):  
Listeria Monocytogenes ◽  
In Vitro Study ◽  
Planktonic Cells ◽  
Free Living ◽  
Biofilm Inhibition ◽  
Additional Tool ◽  
Complete Inactivation ◽  
Gaseous Ozone ◽  
Food Borne

Among food-borne pathogens, Listeria monocytogenes continues to pose concerns to food business operators due to its capacity to form biofilm in processing environments. Ozone may be an eco-friendly technology to control microbial contaminations, but data concerning its effect on Listeria monocytogenes biofilm are still limited. In this study, the effect of gaseous ozone at 50 ppm on planktonic cells and biofilm of reference and food-related Listeria monocytogenes strains was evaluated. Ozone caused a reduction in microbial loads of 3.7 ± 0.4 and 3.9 ± 0.4 Log10 CFU/mL after 10 and 30 min, respectively. A complete inactivation of planktonic cells after 6 h of treatment was observed. Biofilm inhibition and eradication treatments (50 ppm, 6 h) resulted in a significant decrease of the biofilm biomass for 59% of the strains tested, whilst a slight dampening of live cell loads in the biofilm state was observed. In conclusion, gaseous ozone is not sufficient to completely counteract Listeria monocytogenes biofilm, but it may be useful as an additional tool to contrast Listeria monocytogenes free-living cells and to improve the existing sanitization procedures in food processing environments.

Sign in / Sign up

Export Citation Format

Share Document