Germination, growth, and yield of rocket populations show strong ecotypic variation under NaCl stress

AbstractPlant bioregulators (PBRs) represent low-cost chemicals for boosting plant defense, especially under stress conditions. In the present study, redox based PBRs such as thiourea (TU; a non-physiological thiol-based ROS scavenger) and hydrogen peroxide (H2O2; a prevalent biological ROS) were assessed for their ability to mitigate NaCl stress in rice variety IR 64. Despite their contrasting redox chemistry, TU or H2O2 supplementation under NaCl [NaCl + TU (NT) or NaCl + H2O2 (NH)] generated a reducing redox environment in planta, which improved the plant growth compared with those of NaCl alone treatment. This was concomitant with better K+ retention and upregulated expression of NaCl defense related genes including HAK21, LEA1, TSPO and EN20 in both NT and NH treated seedlings. Under field conditions, foliar applications of TU and H2O2, at vegetative growth, pre-flowering and grain filling stages, increased growth and yield attributes under both control and NaCl stress conditions. Principal component analysis revealed glutathione reductase dependent reduced ROS accumulation in source (flag leaves) and sucrose synthase mediated sucrose catabolism in sink (developing inflorescence), as the key variables associated with NT and NH mediated effects, respectively. In addition, photosystem-II efficiency, K+ retention and source-sink relationship were also improved in TU and H2O2 treated plants. Taken together, our study highlights that reducing redox environment acts as a central regulator of plant’s tolerance responses to salt stress. In addition, TU and H2O2 are proposed as potential redox-based PBRs for boosting rice productivity under the realistic field conditions.

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Alleviation of salt stress using gibberellic acid in Chinese cabbage

Acta Agronomica Hungarica ◽

10.1556/aagr.60.2012.4.5 ◽

2012 ◽

Vol 60 (4) ◽

pp. 345-355 ◽

Cited By ~ 2

Author(s):

M. Jamil ◽

M. Ashraf ◽

E. Rha

Keyword(s):

Salt Stress ◽

Gibberellic Acid ◽

Chinese Cabbage ◽

Germination Rate ◽

Harmful Effect ◽

Seed Priming ◽

Nacl Stress ◽

Growth And Yield ◽

Distilled Water ◽

Membrane Injury

Salinity reduces plant growth and yield by affecting morphological and physiological processes. To alleviate the harmful effects of salt stress various approaches involving plant hormones are used. In this study several parameters involving the measurement of cell membrane injury were used to observe whether stress tolerance could be enhanced in Chinese cabbage (B. oleracea capitata L. Chinensis group) by soaking the seeds for 10 h in distilled water (control), or in 100, 150 or 200 mg l−1 gibberellic acid (GA3). The NaCl concentrations were 0 (control), 50, 100 and 150 mM. Seed treated with GA3 showed increased water uptake and decreased electrolyte leakage as compared to that of distilled water-primed seeds even 24 h after soaking under control conditions. Seed priming with GA3 increased the final germination and the germination rate (1/t50, where t50 is the time to 50% germination) under salt stress conditions. Seed priming also alleviated the harmful effect of salt stress on cabbage in terms of fresh and dry weights. Leaf area was higher in plants raised from seeds primed with the higher GA3 concentrations as compared with those raised from seeds treated with distilled water under control conditions (without NaCl) or at 50 mM NaCl stress. The chlorophyll content increased with the NaCl concentration, especially in plants grown from seeds primed with GA3. Plants grown from GA3-primed seeds also suffered lower cellular injury both under control conditions and under NaCl stress.

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Soil amendments with farm yard manure and poultry manure confer tolerance to salt stress in rice (Oryza sativa L.)

Research in Agriculture Livestock and Fisheries ◽

10.3329/ralf.v3i3.30728 ◽

2016 ◽

Vol 3 (3) ◽

pp. 379-386

Author(s):

Md Zulfiker Alam ◽

Debasish Kumar Das ◽

Md Abul Hashem ◽

Md Anamul Hoque

Keyword(s):

Salt Stress ◽

Chlorophyll Content ◽

Crop Production ◽

N Uptake ◽

Poultry Manure ◽

Organic Amendments ◽

Nacl Stress ◽

Growth And Yield ◽

Farm Yard Manure ◽

Rice Varieties

Salinity causes unfavorable environment that restricts normal crop production. Organic amendments could contribute to the improvement of crop production in coastal areas. Two rice varieties viz. BRRI dhan29 (salt-sensitive) and Binadhan-8 (salt-tolerant) were grown in replicated pots to investigate the mitigation potential of salt stress in rice by organic amendments. Two doses of farm yard manure (FYM: 5 and 10 t ha-1) and poultry manure (PM: 4 and 8 t ha-1) were mixed with soils before transplanting. Rice plants were exposed to different concentrations of NaCl (25 and 50 mM) at active tillering stage. Salt stress caused a significant reduction in growth and yield of both rice varieties. Salt stress also decreased reproductive growth, chlorophyll contents, K+/Na+ ratio, nutrient contents and nutrient uptake in both rice varieties. Salinity caused a significant increase in intracellular proline content in BRRI dhan29 but a decrease in Binadhan-8. Organic amendments with FYM and PM resulted in an increase in growth and yield components, chlorophyll content, K+/Na+ ratio and nitrogen (N) uptake. No plants of BRRI dhan29 survived at 50 mM NaCl stress even after addition of FYM and PM. On the other hand, Binadhan-8 conferred tolerance to 50 mM NaCl stress when soils were amended with organic sources, suggesting that cultivation of Binadhan-8 might be profitable in saline affected areas with organic amendments. The present study suggests that organic amendments with FYM and PM confer tolerance to salinity in rice by increasing chlorophyll content, K+/Na+ ratio and N uptake.Res. Agric., Livest. Fish.3(3): 379-386, December 2016

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Physico-biochemical and molecular responses of Acacia auriculiformis to salinity stress

10.1101/837351 ◽

2019 ◽

Author(s):

Deepak B. Gupta ◽

Joydeep Banerjee ◽

Krishnendu Pramanik ◽

Arpita Das ◽

Srikumar Pal

Keyword(s):

Vascular Tissue ◽

Antioxidant Response ◽

Nacl Stress ◽

Tissue Growth ◽

Growth And Yield ◽

Acacia Auriculiformis ◽

Guaiacol Peroxidase ◽

Dependent Manner ◽

Phenol Content ◽

Pal Activity

AbstractSalinity limits the growth and yield of many crops across the globe and is considered as major threat to agriculture. Acacia auriculiformis, an important salt tolerant crop, is growing abundantly in the salt-affected mangrove areas of Sunderban, West Bengal, India. In the present study, we have reported the physiological, molecular and antioxidant response of this crop to a gradient of salt treatments ranging between 0 and 800 mM NaCl. As a stress response, the antioxidant enzymes viz. superoxide dismutase (SOD), catalase (CAT) and guaiacol peroxidase (GPX) were highly activated at 200, 400 and 800 mM NaCl respectively. Antioxidant metabolites such as phenols and thiols elevated with increasing salt treatments thus augmenting antioxidant activity with significant positive relationship with phenol content. Similarly, phenylalanine ammonia lyase (PAL) activity was up-regulated in a dose dependent manner with significant relationship with phenol content. This study also reported the phenolic profile for the first time in A. auriculiformis with the abundance of flavonoids. In addition, transcriptional up-regulation of Na+/H+ antiporter gene (NHX1) and the development of robust vascular tissues was noticed at 400 mM NaCl stress compared to control, while further stress at 800 mM NaCl induced poor vascular tissue growth but with higher PAL activity and consequent higher phenol content. Based on this observation, a model for salt tolerance mechanism of A. auriculiformis has been proposed.

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Pumpkin CmHKT1;1 Controls Shoot Na+ Accumulation via Limiting Na+ Transport from Rootstock to Scion in Grafted Cucumber

International Journal of Molecular Sciences ◽

10.3390/ijms19092648 ◽

2018 ◽

Vol 19 (9) ◽

pp. 2648 ◽

Cited By ~ 4

Author(s):

Jingyu Sun ◽

Haishun Cao ◽

Jintao Cheng ◽

Xiaomeng He ◽

Hamza Sohail ◽

...

Keyword(s):

Salt Tolerance ◽

Ectopic Expression ◽

Nacl Stress ◽

Growth And Yield ◽

Na Transport ◽

Long Distance ◽

Preferential Expression ◽

Long Distance Transport ◽

In The Wild ◽

Yeast Mutants

Soil salinity adversely affects the growth and yield of crops, including cucumber, one of the most important vegetables in the world. Grafting with salt-tolerant pumpkin as the rootstock effectively improves the growth of cucumber under different salt conditions by limiting Na+ transport from the pumpkin rootstock to the cucumber scion. High-affinity potassium transporters (HKTs) are crucial for the long distance transport of Na+ in plants, but the function of pumpkin HKTs in this process of grafted cucumber plants remains unclear. In this work, we have characterized CmHKT1;1 as a member of the HKT gene family in Cucurbita moschata and observed an obvious upregulation of CmHKT1;1 in roots under NaCl stress conditions. Heterologous expression analyses in yeast mutants indicated that CmHKT1;1 is a Na+-selective transporter. The transient expression in tobacco epidermal cells and in situ hybridization showed CmHKT1;1 localization at plasma membrane, and preferential expression in root stele. Moreover, ectopic expression of CmHKT1;1 in cucumber decreased the Na+ accumulation in the plants shoots. Finally, the CmHKT1;1 transgenic line as the rootstock decreased the Na+ content in the wild type shoots. These findings suggest that CmHKT1;1 plays a key role in the salt tolerance of grafted cucumber by limiting Na+ transport from the rootstock to the scion and can further be useful for engineering salt tolerance in cucurbit crops.

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Growth and yield of okra (Abelmoschus esculentus (L.) Moench) grown in the marginal upland area of Sta. Rita, Samar as influenced by different planting densities and mulching materials

Annals of Tropical Research ◽

10.32945/atr39sa4.2017 ◽

2017 ◽

pp. 44-54

Author(s):

Zenaida Gonzaga ◽

Warren Obeda ◽

Ana Linda Gorme ◽

Jessie Rom ◽

Oscar Abrantes ◽

...

Keyword(s):

Bare Soil ◽

Abelmoschus Esculentus ◽

Growth And Yield ◽

Planting Density ◽

Rice Hull ◽

Plastic Mulch ◽

Lady’S Finger ◽

Materials Used ◽

Set Up ◽

A Minor

Okra or Lady’s finger, botanically known as Abelmoschus esculentus (L.) Moench, is a tropical and sub-tropical indigenous vegetable crop commonly grown for its fibrous, slimy, and nutritious fruits and consumed by all classes of population. It has also several medicinal and economic values. Despite its many uses and potential value, its importance is under estimated, under-utilized, and considered a minor crop and little attention was paid to its improvement. The study was conducted to evaluate the effects of different planting densities and mulching materials on the growth and yield of okra grown in slightly sloping area in the marginal uplands in Sta. Rita, Samar, Philippines. A split-plot experiment was set up with planting density as main plot and the different mulching materials as the sub-plot which were: unmulched or bare soil, rice straw, rice hull, hagonoy and plastic mulch. Planting density did not significantly affect the growth and yield of okra. Regardless ofthe mulching materials used, mulched plants were taller and yielded higher compared to unmulched plants. Moreover, the use of plastic mulch resulted to the highest total fruit yield. The results indicate the potential of mulching in increasing yield and thus profitability of okra production under marginal upland conditions.

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Response of corn (Zea mays L.) to various organic-based fertilizers in marginal upland of Sta. Rita, Samar

Annals of Tropical Research ◽

10.32945/atr39sa3.2017 ◽

2017 ◽

pp. 31-43

Author(s):

Berta Ratilla ◽

Loreme Cagande ◽

Othello Capuno

Keyword(s):

Block Design ◽

Management Strategies ◽

Chemical Properties ◽

Organic Fertilizers ◽

Growth And Yield ◽

Physical And Chemical Properties ◽

Total N ◽

Randomized Complete Block Design ◽

Physical And Chemical ◽

And Chemical Properties

Organic farming is one of the management strategies that improve productivity of marginal uplands. The study aimed to: (1) evaluate effects of various organic-based fertilizers on the growth and yield of corn; (2) determine the appropriate combination for optimum yield; and (3) assess changes on the soil physical and chemical properties. Experiment was laid out in Randomized Complete Block Design, with 3 replications and 7 treatments, namely; T0=(0-0-0); T1=1t ha-1 Evans + 45-30-30kg N, P2O5, K2O ha-1; T2=t ha-1 Wellgrow + 45-30-30kg N, P2O5, K2O ha-1; T3=15t ha-1 chicken dung; T4=10t ha-1 chicken dung + 45-30-30kg N, P2O5, K2O ha-1; T5=15t ha-1 Vermicast; and T6=10t ha-1 Vermicast + 45-30-30kg N, P2O5, K2O ha-1. Application of organic-based fertilizers with or without inorganic fertilizers promoted growth of corn than the control. But due to high infestation of corn silk beetle(Monolepta bifasciata Horns), its grain yield was greatly affected. In the second cropping, except for Evans, any of these fertilizers applied alone or combined with 45-30-30kg N, P2O5, K2O ha-1 appeared appropriate in increasing corn earyield. Soil physical and chemical properties changed with addition of organic fertilizers. While bulk density decreased irrespective of treatments, pH, total N, available P and exchangeable K generally increased more with chicken dung application.

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