Rheumatoid factors, anti-filaggrin antibodies and low in vitro interleukin-2 and interferon-γ production are useful immunological markers for early diagnosis of community cases of rheumatoid arthritis. A preliminary study

2001 ◽  
Vol 68 (2) ◽  
pp. 144-153 ◽  
Author(s):  
Olivier Vittecoq ◽  
Fabienne Jouen-Beades ◽  
Kataryna Krzanowska ◽  
Isabelle Bichon-Tauvel ◽  
Jean-François Ménard ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Early Diagnosis ◽  
Interleukin 2 ◽  
Interferon Γ ◽  
Rheumatoid Factors ◽  
Immunological Markers ◽  
Preliminary Study

scholarly journals Age-related aspects of cytokine profile of immune response in children with pertussis

2020 ◽  
Vol 65 (3) ◽  
pp. 84-90
Author(s):  
O. P. Popova ◽  
M. S. Blyakher ◽  
I. M. Fedorova ◽  
S. I. Koteleva ◽  
I. V. Kapustin
Keyword(s):  
Interleukin 2 ◽  
Humoral Response ◽  
Interleukin 10 ◽  
Interferon Γ ◽  
Interleukin 4 ◽  
Cytokine Network ◽  
Immunological Markers ◽  
Age Related ◽  
Immunoregulatory Cytokines ◽  
The Relationship

The article presents a comparative analysis of the cytokine network in 161 patients with pertussis under the age of 1 year and in 180 patients over the age of 1 year. The studies revealed lower production of immunoregulatory cytokines (interferon-γ and interleukin-2) in patients under the age of 1 year at all stages of the disease and with all variants of pertussis (both mono- and mixed infections). The researchers revealed the relationship between the level of interferon-γ production and the severity of pertussis. They revealed age differences in the interferon-8 production in patients with mixed infection, which can determine clinical features and cause bronchopulmonary complications. The authors demonstrated the features of the dynamics of spontaneous and induced production of interleukin-6 and anti-inflammatory cytokines (interleukin-4, interleukin-10) in children under the age of 1 year; these features can be considered as immunological markers determining the imperfection of the humoral response in patients with pertussis in this age group.

scholarly journals Plasma Levels of Eicosapentaenoic Acid Are Associated with Anti-TNF Responsiveness in Rheumatoid Arthritis and Inhibit the Etanercept-driven Rise in Th17 Cell Differentiationin Vitro

2017 ◽  
Vol 44 (6) ◽  
pp. 748-756 ◽  
Author(s):  
Louisa Jeffery ◽  
Helena L. Fisk ◽  
Philip C. Calder ◽  
Andrew Filer ◽  
Karim Raza ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Fatty Acid ◽  
Eicosapentaenoic Acid ◽  
Th17 Cell ◽  
Th17 Cells ◽  
Interferon Γ ◽  
Interleukin 17 ◽  
Differentiationin Vitro

Objective.To determine whether levels of plasma n-3 polyunsaturated fatty acids are associated with response to antitumor necrosis factor (anti-TNF) agents in rheumatoid arthritis (RA), and whether this putative effect may have its basis in altering anti-TNF–driven Th17 cell differentiation.Methods.Plasma was collected at baseline and after 3 months of anti-TNF treatment in 22 patients with established RA, and fatty acid composition of the phosphatidylcholine (PC) component was measured. CD4+CD25− T cells and monocytes were purified from the blood of healthy donors and cocultured in the presence of anti-CD3, with or without etanercept (ETN), eicosapentaenoic acid (EPA), or the control fatty acid, linoleic acid (LA). Expression of interleukin 17 and interferon-γ was measured by intracellular staining and flow cytometry.Results.Plasma PC EPA levels and the EPA/arachidonic acid ratio correlated inversely with change in the Disease Activity Score at 28 joints (DAS28) at 3 months (−0.51, p = 0.007 and −0.48, p = 0.01, respectively), indicating that higher plasma EPA was associated with a greater reduction in DAS28. Plasma PC EPA was positively associated with European League Against Rheumatism response (p = 0.02). An increase in Th17 cells post-therapy has been associated with nonresponse to anti-TNF. ETN increased Th17 frequenciesin vitro. Physiological concentrations of EPA, but not LA, prevented this.Conclusion.EPA status was associated with clinical improvements to anti-TNF therapyin vivoand prevented the effect of ETN on Th17 cellsin vitro. EPA supplementation might be a simple way to improve anti-TNF outcomes in patients with RA by suppressing Th17 frequencies.

Dressed to kill? A review of why antiviral CD8 T lymphocytes fail to prevent progressive immunodeficiency in HIV-1 infection

Blood ◽  
2001 ◽  
Vol 98 (6) ◽  
pp. 1667-1677 ◽  
Author(s):  
Judy Lieberman ◽  
Premlata Shankar ◽  
N. Manjunath ◽  
Jan Andersson
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Lymphocytes ◽  
Cd8 T Cells ◽  
Interleukin 2 ◽  
Interferon Γ ◽  
Short Term Exposure ◽  
Infected Cells ◽  
Hiv 1

Abstract CD8 T cells play an important role in protection and control of HIV-1 by direct cytolysis of infected cells and by suppression of viral replication by secreted factors. However, although HIV-1–infected individuals have a high frequency of HIV-1–specific CD8 T cells, viral reservoirs persist and progressive immunodeficiency generally ensues in the absence of continuous potent antiviral drugs. Freshly isolated HIV-specific CD8 T cells are often unable to lyse HIV-1–infected cells. Maturation into competent cytotoxic T lymphocytes may be blocked during the initial encounter with antigen because of defects in antigen presentation by interdigitating dendritic cells or HIV-infected macrophages. The molecular basis for impaired function is multifactorial, due to incomplete T-cell signaling and activation (in part related to CD3ζ and CD28 down-modulation), reduced perforin expression, and inefficient trafficking of HIV-specific CD8 T cells to lymphoid sites of infection. CD8 T-cell dysfunction can partially be corrected in vitro with short-term exposure to interleukin 2, suggesting that impaired HIV-specific CD4 T helper function may play a significant causal or exacerbating role. Functional defects are qualitatively different and more severe with advanced disease, when interferon γ production also becomes compromised.

Effects of folic acid supplement on uterine prostaglandin metabolism and interleukin-2 expression on day 15 of gestation in white breed and crossbred Meishan sows

2004 ◽  
Vol 84 (1) ◽  
pp. 63-72 ◽  
Author(s):  
Frédéric Guay, J. Jacques Matte ◽  
Christiane L. Girard ◽  
Marie-France Palin, Alain Giguère ◽  
Jean-Paul Laforest
Keyword(s):  
Folic Acid ◽  
Transforming Growth Factor ◽  
Interleukin 2 ◽  
Transforming Growth Factor Β ◽  
Interferon Γ ◽  
Prostaglandin E ◽  
Cyclooxygenase 1 ◽  
White Breed ◽  
Uterine Environment

The effects of a dietary folic acid (B9) supplement on the uterine environment of nulliparous Yorkshire- Landrace (YL) and multiparous Landrace (LD) and multiparous Meishan-Landrace (ML) sows were investigated. Sows were randomly assigned to two treatments: 0 mg kg-1 and 15 mg kg-1 of B9. The supplements were given daily from the estrus preceding insemination up to slaughter on Day 15 of gestation. At slaughter, one uterine horn was used to collect the “uterine flush” and conceptuses in order to determine the uterine content of prostaglandin E2(PGE2) and F2α(PGF2α), estradiol-17β (E2) and transforming growth factor-β2 (TGF-β2), and conceptus expression of cytochrome P450aromatase (P450) and interferon γ mRNA (IFNγ). The other horn was used to determine endometrial expression of interleukin-2 (IL-2), cyclooxygenase-1 (COX1) and -2 (COX2) mRNA and to evaluate endometrial in vitro secretion of PGE2. The B9 supplement tended to decrease the uterine content of PGE2 (P < 0.08), and decrease endometrial expression of COX1 mRNA (P < 0.05). The in vitro secretion of PGE2 was reduced by B9 supplement only in YL sows (P < 0.05). The type of sow did not have any effect on the uterine content of PGE2 (P > 0.10). However, endometrial expression of COX2 mRNA was lower for YL than LD sows (P < 0.05), but there was no difference between ML and LD sows. Endometrial expression of COX1 mRNA was higher for ML than LD sows (P < 0.05). The B9 supplement tended to decrease uterine content of E2 and TGF-β2 in YL and LD sows (P < 0.07), but conceptus expression of P450 mRNA increased only for YL sows (P < 0.05). The uterine content of E2 was lower for YL than LD sows (P < 0.05). The B9 supplement decreased endometrial expression of IL-2 mRNA in LD and YL sows ( P < 0.05), but increased it in ML sows (P < 0.05). Conceptus expression of IFNγ mRNA was not affected either by B9 supplementation or the type of sow. In conclusion, the effect of B9 supplementation on the porcine uterine environment at Day 15 of gestation was influenced by sow type (genotype and/or parity). Key words: Sow, gestation, folic acid, prostaglandin, interleukin-2

Targeting Lymphocyte Activation Gene 3 to Reverse T-Lymphocyte Dysfunction and Improve Survival in Murine Polymicrobial Sepsis

2020 ◽  
Vol 222 (6) ◽  
pp. 1051-1061
Author(s):  
Jing-sheng Lou ◽  
Jia-feng Wang ◽  
Miao-miao Fei ◽  
Yan Zhang ◽  
Jun Wang ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cell Apoptosis ◽  
Interleukin 2 ◽  
Lymphocyte Activation ◽  
Interferon Γ ◽  
T Cell Apoptosis ◽  
Cytokine Levels ◽  
Lymphocyte Activation Gene

Abstract Background Lymphocyte activation gene 3 (LAG-3) is one of the immune checkpoint molecules, negatively regulating the T-cell reactions. The present study investigated the role of LAG-3 in sepsis-induced T-lymphocyte disability. Methods Mice sepsis was induced by cecal ligation and puncture (CLP). LAG-3 expression on some immune cells were detected 24 hours after CLP. LAG-3 knockout and anti–LAG-3 antibody were applied to investigate the effects on the survival, bacterial clearance. Cytokine levels, T-cell counts, and the presence of apoptosis (in blood, spleen, and thymus) were also determined. In vitro T-cell apoptosis, interferon γ secretion, and proliferation were measured. The expression of interleukin 2 receptor on T cells was also determined after CLP. Results LAG-3 was up-regulated on CD4+/CD8+ T, CD19+ B, natural killer, CD4+CD25+ regulatory T cells and dendritic cells. Both LAG-3 knockout and anti–LAG-3 antibody had a positive effect on survival and on blood or peritoneal bacterial clearance in mice undergoing CLP. Cytokine levels and T-cell apoptosis decreased in anti–LAG-3 antibody–treated mice. Induced T-cell apoptosis decreased, whereas interferon γ secretion and proliferation were improved by anti–LAG-3 antibody in vitro. Interleukin 2 receptor was up-regulated on T cells in both wild-type and LAG-3–knockout mice undergoing CLP. Conclusions LAG-3 knockout or anti–LAG-3 antibody blockade protected mice undergoing CLP from sepsis-associated immunodysfunction and may be a new target for the treatment.

scholarly journals Synergy among receptors on resting NK cells for the activation of natural cytotoxicity and cytokine secretion

Blood ◽  
2006 ◽  
Vol 107 (1) ◽  
pp. 159-166 ◽  
Author(s):  
Yenan T. Bryceson ◽  
Michael E. March ◽  
Hans-Gustaf Ljunggren ◽  
Eric O. Long
Keyword(s):  
Nk Cells ◽  
Interleukin 2 ◽  
Interferon Γ ◽  
Calcium Flux ◽  
Target Cells ◽  
Natural Cytotoxicity ◽  
Tnf Α ◽  
Specific Pair ◽  
Factor Α

Abstract Freshly isolated, resting natural killer (NK) cells are generally less lytic against target cells than in vitro interleukin 2 (IL-2)-activated NK cells. To investigate the basis for this difference, the contribution of several receptors to activation of human NK cells was examined. Target-cell lysis by IL-2-activated NK cells in a redirected, antibody-dependent cytotoxicity assay was triggered by a number of receptors. In contrast, cytotoxicity by resting NK cells was induced only by CD16, and not by NKp46, NKG2D, 2B4 (CD244), DNAM-1 (CD226), or CD2. Calcium flux in resting NK cells was induced with antibodies to CD16 and, to a weaker extent, antibodies to NKp46 and 2B4. Although NKp46 did not enhance CD16-mediated calcium flux, it synergized with all other receptors. 2B4 synergized with 3 other receptors, NKG2D and DNAM-1 each synergized with 2 other receptors, and CD2 synergized with NKp46 only. Resting NK cells were induced to secrete tumor necrosis factor α (TNF-α) and interferon γ (IFN-γ), and to kill target cells by engagement of specific, pair-wise combinations of receptors. Therefore, natural cytotoxicity by resting NK cells is induced only by mutual costimulation of nonactivating receptors. These results reveal distinct and specific patterns of synergy among receptors on resting NK cells.

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