scholarly journals Chromosomal and extrachromosomal transmission of cellular melanization in the Freckled phenotype in Drosophila melanogaster

1968 ◽  
Vol 11 (2) ◽  
pp. 141-150
Author(s):  
C. Barigozzi ◽  
M. Sari Gorla
Keyword(s):  
Drosophila Melanogaster ◽  
Adult Stage ◽  
Pupal Stage ◽  
Double Dose ◽  
Dominant Mutant

Freckled (Frd) is a dominant mutant of D. melanogaster, lethal in double dose, which manifests itself by melanization in the adult stage, while nothing can be noticed in the larva and/or in the pupa. Its localization has been determined at nearly 102 of the second chromosome.Among the offspring from backcrosses involving Frd, some individuals that do not carry the Frd gene exhibit a weak Frd manifestation in the adult stage, or a stronger one, mostly lethal, in the larval or pupal stage. Weak Frd, indicated as [Frd], is transmitted indefinitely without any recognizable linkage with the chromosomes, although its frequency is influenced by the 1st and, probably, by the 3rd chromosome. No matroclinous transmission has been found. It is concluded that [Frd] is probably controlled by an extrachromosomal entity, not located in the cytoplasm. The possible relationships between Frd and [Frd] are discussed.

The Post-embryonic Development of the Tracheal System in Drosophila melanogaster

1957 ◽  
Vol s3-98 (41) ◽  
pp. 123-150
Author(s):  
JOAN M. WHITTEN
Keyword(s):  
Drosophila Melanogaster ◽  
Adult Stage ◽  
Larval Instar ◽  
Pupal Stage ◽  
Tracheal System ◽  
True Nature ◽  
The Third ◽  
Air Sacs ◽  
Abdominal Region ◽  
Pupal Cuticle

The fate of the tracheal system is traced from the first larval instar to the adult stage. The basic larval pattern conforms to that shown for other Diptera Cyclorrhapha (Whitten, 1955), and is identical in all three instars. According to previous accounts the adult system directly replaces the larval: the larval system is partly shed, partly histolysed, and the adult system arises from imaginal cell clusters independently of the preceding larval system. In contrast, it is shown here that in the cephalic, thoracic, and anterior abdominal region there is a definite continuity in the tracheal system, from larval, through pupal to the adult stage, whereas in the posterior abdominal region the larval system is histolysed, and the adult system is independent of it in origin. Moreover, in the pupal stage this region is tracheated by tracheae arising from the anterior abdominal region and belonging to a distinct pupal system. Moulting of the tracheal linings is complete at the first and second larval ecdyses, but incomplete at the third larval-pupal and pupal-adult ecdyses. In consequence, in both pupal and adult systems there are tracheae which are secreted around preexisting tracheae, others formed as new ‘branch’ tracheae, and those which have been carried over from the previous instar. In the adult the newly formed tracheae of the posterior abdominal region fall into a fourth category. Most of the adult thoracic air sacs correspond to new ‘branch’ tracheae of other instars. The pre-pupal moult and instar are discussed with reference to the tracheal system and tentative suggestions are made concerning the true nature of the pre-pupal cuticle. There is no pre-pupal tracheal system. Events traced for Drosophila would seem to be general for Cyclorrhapha, both Acalypterae and Calypterae. The separate fates of the anterior and posterior abdom inal systems, in contrast with the straightforward development in Dipterc Nematocera, would appear to mark a distinct step in the evolution of the system in Diptera.

scholarly journals Further studies on pre-imaginal olfactory conditioning in insects

1939 ◽  
Vol 127 (848) ◽  
pp. 424-433 ◽  
Keyword(s):  
Drosophila Melanogaster ◽  
High Mortality ◽  
Adult Stage ◽  
Pupal Stage ◽  
Olfactory Response ◽  
Significant Preference ◽  
Olfactory Conditioning ◽  
Odoriferous Substances ◽  
Conditioning Effect ◽  
Wax Moth

In the course of an earlier paper (Thorpe and Jones 1937), it was shown that the ichneumonid parasite Nemeritis canescens (Grav.) can be induced to develop a positive olfactory response to the odour of an abnormal host, the wax moth Meliphora grisella (F.), by rearing it artificially upon that host. It was shown that the change in the olfactory response brought about by this process was due, in large part, to conditioning taking place in the adult stage immediately after emergence from the pupa. This was confirmed by the fact that Nemeritis , reared on the normal host Ephestia , can be made positively responsive to the odour of Meliphora merely by placing them immediately on emergence in an apparatus through which is pumped a stream of air which has previously passed over a number of living Meliphora larvae. But the conditioning, obtained by treatment of the newly emerged adult in this manner, is not usually as strong as that produced by actually rearing the insects on the abnormal host. From this it was concluded that part at least of the conditioning effect produced by rearing on the wax moth larvae must be the result of the influence of the host acting during the pre-imaginal period. Attempts to get conclusive demonstrations of this in Nemeritis were, however, ineffective. It was hoped that it would be possible, having reared the parasite to the pupal stage on the wax moth, to dissect it from its cocoon, which of course may be presumed to be contaminated with the odour of the host. For if pupae, having been thus dissected out and then washed and completely isolated from the odour of the wax moth, still show significant preference for it on emergence, the existence of pre-imaginal conditioning could be considered firmly established. Unfortunately, the high mortality caused when pupae are treated in this way made the experiment impracticable. Many parasites can be removed from their cocoons without suffering harm, but Nemeritis appears much more susceptible to injury than most. The object of the present work was to perform a similar experiment on some insect which does not spin a cocoon and in which the puparia can therefore be washed free of all contaminating odoriferous substances. Because of its convenience as a laboratory insect Drosophila melanogaster Mg. ( fasciata Mg., ampelophila Loew.) was chosen for this purpose (a strain being kindly supplied by Dr C. H. Waddington). The two sexes were used indiscriminately since in some preliminary control experiments no significant differences in their behaviour were observed. In a second paper (Thorpe 1938), it was shown that in Nemeritis a positive conditioning can be produced, not merely to the odour of a possible host, but to the odour of such biologically abnormal substances as cedar wood oil. It was felt that Drosophila also offered a particularly suitable subject for further experiments of this nature in that its rearing on synthetic foodstuff lias long been standardized. Odoriferous substances can easily be mixed with the food, thus exposing the larva to their influence over the whole of its life. The Drosophila were reared on Pearl’s S. 101 medium, modified in certain particulars by Mr J. H. Sang, for whose help in the matter I am much indebted. The exact composition of the medium is as follows:

GROWTH AND DEVELOPMENT OF LARVAE OF THE GRANARY WEEVIL, SITOPHILUS GRANARIUS (COLEOPTERA: CURCULIONIDAE), ON NATURAL AND MERIDIC DIETS

1973 ◽  
Vol 105 (2) ◽  
pp. 249-256 ◽  
Author(s):  
J. E. Baker ◽  
J. M. Mabie
Keyword(s):  
Wheat Flour ◽  
Growth Rates ◽  
Adult Stage ◽  
Larval Growth ◽  
Pupal Stage ◽  
Sitophilus Granarius ◽  
Granary Weevil ◽  
Whole Wheat Flour ◽  
Whole Wheat ◽  
Meridic Diet

AbstractA procedure is described for obtaining free eggs and first-stage larvae of Sitophilus granarius (L.) and rearing the larvae to the adult stage on whole wheat, corn, and rice flours and on diet 2, a casein–starch-based meridic diet.Larval growth rates of males and females reared at 29 °C and 65% R.H. on whole wheat flour were comparable although males attained a higher maximum mean weight. Days to 50% adult emergence (eclosion) were 25.5 and 26.5 for females and males, respectively.Larval growth rates on whole wheat flour were comparable with those on whole corn flour but were better than the rates obtained on whole rice flour; however, growth on diet 2 was improved compared with that on whole wheat flour. The mean weights of 14-day-old larvae were 2.51 ±.40 mg and 1.25 ±.16 mg for those reared on diet 2 and wheat flour, respectively. Similarly, survival to the adult stage was slightly greater (79%) on diet 2 than on wheat (70%). On diet 1, a casein–glucose-based meridic diet, no larvae developed to the pupal stage.

SEASONAL DEVELOPMENT AND HABITS OF PSEUDOPITYOPHTHORUS SPP. (COLEOPTERA: SCOLYTIDAE) IN SOUTHERN OHIO

1969 ◽  
Vol 101 (3) ◽  
pp. 306-313 ◽  
Author(s):  
Charles O. Rexrode
Keyword(s):  
Larval Stage ◽  
Bark Beetles ◽  
Adult Stage ◽  
Average Length ◽  
Pupal Stage ◽  
Seasonal Development ◽  
Two Generations ◽  
Spring Emergence ◽  
Seasonal History ◽  
Initial Entry

AbstractDuring 1965–67, the seasonal history and habits of two oak bark beetles, Pseudopityophthorus minutissimus (Zimm.) and P. pruinosus (Eichhoff), were studied in 47 oaks, Quercus spp., in southern Ohio.All study trees were attacked and the male beetles made initial entry into the trees. The attack period per tree was usually 1–3 days, and the density of attack varied from 5 to 120 galleries 1 ft2 of surface area. Oviposition began as soon as egg gallery construction began, and the incubation period was about 5 days in the summer. The rate of egg gallery construction was about 0.2 in./day in mid-summer. The average length of the egg galleries was 1.9 cm and the average number of eggs per gallery was 41.5. Over 50% of the parent adults of both species re-emerged after egg gallery construction and oviposition. The re-emerging parent adults can attack, lay eggs, and re-emerge three times. The larval stage can last 25–247 days, the pupal stage 15–139 days, and the adult stage 7–198 days. The beetles can overwinter in every stage but the pupal stage. Spring emergence occurred 29 April in 1966 and 14 April in 1967, and in southern Ohio, two generations and a partial one occur each year.Trap trees were attacked 7–205 days and brood emergence lasted 58–329 days. The beetles can attack one tree three times.

scholarly journals Tissue-specific expression of the heat shock protein HSP27 during Drosophila melanogaster development.

1990 ◽  
Vol 111 (3) ◽  
pp. 817-828 ◽  
Author(s):  
D Pauli ◽  
C H Tonka ◽  
A Tissieres ◽  
A P Arrigo
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Stress Protein ◽  
Pupal Stage ◽  
Adult Brain ◽  
Proliferating Cells ◽  
Specific Expression ◽  
The Central Nervous System

The alpha-crystallin-related heat shock (stress) protein hsp27 is expressed in absence of heat shock during Drosophila melanogaster development. Here, we describe the tissue distribution of this protein using an immunoaffinity-purified antibody. In embryos, hsp27 translated from maternal RNA is uniformly distributed, except in the yolk. During the first, second, and early third larval stages, hsp27 expression is restricted to the brain and the gonads. These tissues are characterized by a high level of proliferating cells. In late third instar larvae and early pupae, in addition to the central nervous system and the gonads, all the imaginal discs synthesize hsp27. The disc expression seems restricted to the beginning of their differentiation since it disappears during the second half of the pupal stage: no more hsp27 is observed in the disc-derived adult organs. In adults, hsp27 is still present in some regions of the central nervous system, and is also expressed in the male and female germ lines where it accumulates in mature sperm and oocytes. The transcript and the protein accumulate in oocytes since the onset of vitellogenesis with a uniform distribution similar to that found in embryos. The adult germ lines transcribe hsp27 gene while no transcript is detected in the late pupal and adult brain. These results suggest multiple roles of hsp27 during Drosophila development which may be related to both the proliferative and differentiated states of the tissues.

scholarly journals Study on the biology of groundnut bruchid, Caryedon serratus Olivier [Coleoptera: Bruchidae] on stored groundnut in Ganye area, Adamawa State, Nigeria

2020 ◽  
Vol 12 (3) ◽  
pp. 272-276
Author(s):  
C.S. Oaya
Keyword(s):  
Life Cycle ◽  
Larval Stage ◽  
Developmental Stages ◽  
Adult Stage ◽  
Pupal Stage ◽  
Male And Female ◽  
Incubation Stage ◽  
Caryedon Serratus ◽  
The Mean ◽  
Total Life

Abstract. Study on the biology of groundnut bruchid, Caryedon serratus Olivier was carried out in the Laboratory of the Department of Agricultural Technology, Adamawa State College of Agriculture, Ganye, Adamawa State from June to September, 2015. The sex of the bruchid (male and female) was assigned the sub-treatment while the period of development was the main treatment and was replicated three times. The work considered the pre-oviposition stage, incubation stage, larval stage, pupal stage, adult stage and the total life cycle of the bruchid (male and female). The experiment was carried out under Laboratory conditions at 30-35°C temperature and 70-90% relative humidity. After the experiment was established, weekly counting of the total number of eggs laid was carried out at intervals. The results showed that, the mean developmental stages in both female and male bruchids were: 6.50 and 6.00 days; 17.00 and 18.00 days; 13.50 and 13.50 days; 12.00 and 13.50 days; 48.00 and 50.00 days for incubation stage, larval stage, pupal stage, adult stage and total life cycle of the bruchids, respectively. Moreover, the mean pre-oviposition and oviposition stages in female adult bruchid were 1.50 and 5.50 days, respectively.

scholarly journals STUDIES ON THE SEX-SPECIFIC LETHALS OF DROSOPHILA MELANOGASTER. II. FURTHER STUDIES ON A MALE-SPECIFIC LETHAL GENE, MALELESS

Genetics ◽  
1976 ◽  
Vol 84 (2) ◽  
pp. 257-266
Author(s):  
Atsumi Tanaka ◽  
Akihiro Fukunaga ◽  
Kugao Oishi
Keyword(s):  
Drosophila Melanogaster ◽  
Larval Stage ◽  
Maternal Effect ◽  
Sex Differentiation ◽  
Pupal Stage ◽  
Imaginal Discs ◽  
Lethal Gene ◽  
Male Specific Lethal ◽  
Male Specific

ABSTRACT Effects of a second chromosome male-specific lethal gene, maleless (mle), of Drosophila melanogaster were further studied. It was shown that, although no maternal effect was seen with respect to the male-specific lethality, the lethal stage was influenced by whether parental females were homozygous or heterozygous for mle. Thus, in the former mle/mle males died mostly in the late third instar larval stage, while in the latter practically all males survived to the pupal stage. In the dying mle/mle male pupae complete differentiation of adult external head and thorax structures was often observed but that of abdominal structures was incomplete forming only a few segments in most cases. Imaginal discs from third instar mle/mle male larvae which were produced by mle/mle mothers and were destined to die as larvae were able to differentiate into adult structures upon transplantation into normal third instar larval hosts.—A somewhat elaborated version of the previously presented hypothesis (Fukunaga, Tanaka and Oishi 1975) was discussed as to the possible presence of a class of sex-specific lethals which are not related to the process of primary sex differentiation

scholarly journals Valorisation of the Effects of Bioactive Compounds of the Ethanolic Extract of Ramalina Farinacea (Ramalinaceae) on the Development, Eating and Pupation Behavior of Drosophila Melanogaster (Diptera: Drosophilidae)

2021 ◽  
Vol 8 (4) ◽  
pp. 113-120
Author(s):  
Fatma Zohra Saadane ◽  
Nour El Imene Boublata ◽  
Sarra Habbachi ◽  
Abir Bouzar ◽  
Wafa Habbachi ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Secondary Metabolites ◽  
Adult Stage ◽  
Ethanolic Extract ◽  
Delayed Effects ◽  
Slowing Down ◽  
Vinegar Fly ◽  
Pupation Behavior ◽  
The Impact ◽  
Ramalina Farinacea

Plants are rich in bioactive chemical secondary metabolites and have proven insecticidal activity by killing or repelling insects. In this work, we aim to evaluate the direct and delayed effects of ethanolic plant extracts on the vinegar fly (Drosophila melanogaster). The treatment was performed by ingestion on second instar larvae (L2) to evaluate the impact of the ethanolic extract on development for 15 days and subsequently on the feeding behavior of the larvae. The results of this study indicate a slowing down of pupal growth until the adult stage, at the three concentrations (0.25 µg/ml, 0.5 µg/ml, 1.5 µg/ml, 2 µg/ml) used. The results also showed that after three days of treatment, third instar D. melanogster larvae lost the ability to detect the odors of their nutrient environments. Other numbers of larvae (34 %) do not make a choice in the different tests performed. This study indicates that the ethanolic extract of Ramalina farinacea has a neurotoxic property our results confirmed the presence of toxic secondary metabolites which have bioinsecticidal activities in this extract.

scholarly journals Analysis of Drosophila melanogaster reproduction and preadult mortality after the influence of microwave radiation

2020 ◽  
Keyword(s):  
Drosophila Melanogaster ◽  
Mortality Rate ◽  
Electromagnetic Radiation ◽  
Microwave Radiation ◽  
Pupal Stage ◽  
Embryonic Mortality ◽  
Adult Offspring ◽  
Short Term ◽  
Short Term Exposure ◽  
The Impact

A new type of anthropogenic impact – low-intensity electromagnetic radiation of extremely high frequencies (EHF EMR) demonstrates various genetic effects. The questions of the organism adaptive response formation to short-term exposure of EMR that depends on the individual’s genotype are of particular interest. The objective of this study was to analyze the reproductive ability and preadult mortality in Drosophila melanogaster with whiteapticot mutation after the influence of microwave radiation. We used D. melanogaster stocks that carry whiteapticot mutation, but differ in the genetic background on which this mutation is located: wa(C-S), wa(Or) and wa. Virgin flies were irradiated. The parameters of the external influence were power flux density W=10 μW/cm2, frequency F=65 GHz, exposure time t=5 minutes. The stages of embryo death, number of adult offspring, and mortality rate at the pupal stage were analyzed. The results of the study showed that the effect of electromagnetic radiation on virgin imagoes of Drosophila with an impaired tryptophan metabolism modifies the survival rate of the offspring at the preimaginal stages of ontogenesis. In the offspring of young flies (at the age of 3–8 days) the frequency of embryonic mortality decreases during the 0–5.5 hours period (initial stages of cleavage and blastoderm formation) and 5.5–17 hours period (stage of gastrulation and embryo segmentation, histogenesis) of embryogenesis. The total number of offspring at the adult stage does not differ from the control values; the mortality rate at the pupal stage also does not change in the offspring of young parents after exposure to EMR. An increase in the age of parental couples that were exposed to short-term EMR on the first day after eclosion from pupae (20–25 days aged) leads to a decrease in the embryonic mortality rate of offspring during the 17–22 hours of embryogenesis (organogenesis stage and larval exit from chorion). The total embryonic mortality rate is determined solely by the age of the parents. The strength of this factor for the stocks is h2wa=69.7 %, h2wa(C-S)=52.2 % and h2wa(Or)=64.9 % respectively. The impact of EMR affects the embryonic mortality rate only for the wa(Or) (h2EMR=18.3 %). External exposure does not change the number of imago offspring in individuals aged 0–5 days; the number of adult offspring increased by 1,2 times in individuals aged 20–25 days in the stock wa(C-S). A decrease in the number of dead individuals at the pupal stage in the descendants of 20–25-day-old parents after exposure to EMR EHF on average by three times was shown.

scholarly journals Transgenerational effects on development following microplastic exposure in Drosophila melanogaster

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11369
Author(s):  
Eva Jimenez-Guri ◽  
Katherine E. Roberts ◽  
Francisca C. García ◽  
Maximiliano Tourmente ◽  
Ben Longdon ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Life History ◽  
Polyvinyl Chloride ◽  
Life History Traits ◽  
Pupal Stage ◽  
Life Cycles ◽  
Adult Size ◽  
Chemical Additives ◽  
Plastic Pollution ◽  
Marine Realm

Background Plastic pollution affects all ecosystems, and detrimental effects to animals have been reported in a growing number of studies. However, there is a paucity of evidence for effects on terrestrial animals in comparison to those in the marine realm. Methods We used the fly Drosophila melanogaster to study the effects that exposure to plastics may have on life history traits and immune response. We reared flies in four conditions: In media containing 1% virgin polyethylene, with no chemical additives; in media supplemented with 1% or 4% polyvinyl chloride, known to have a high content of added chemicals; and control flies in non-supplemented media. Plastic particle size ranged from 23–500 µm. We studied fly survival to viral infection, the length of the larval and pupal stage, sex ratios, fertility and the size of the resultant adult flies. We then performed crossings of F1 flies in non-supplemented media and looked at the life history traits of the F2. Results Flies treated with plastics in the food media showed changes in fertility and sex ratio, but showed no differences in developmental times, adult size or the capacity to fight infections in comparison with controls. However, the offspring of treated flies reared in non-supplemented food had shorter life cycles, and those coming from both polyvinyl chloride treatments were smaller than those offspring of controls.

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