Comparison of prevalence estimation ofMycobacterium aviumsubsp.paratuberculosisinfection by sampling slaughtered cattle with macroscopic lesionsvs.systematic sampling

2012 ◽  
Vol 141 (7) ◽  
pp. 1536-1544 ◽  
Author(s):  
J. ELZE ◽  
E. LIEBLER-TENORIO ◽  
M. ZILLER ◽  
H. KÖHLER
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Avium ◽  
Random Sampling ◽  
Detection Rate ◽  
Clinical Signs ◽  
Systematic Sampling ◽  
Systematic Random Sampling ◽  
Prevalence Estimation

SUMMARYThe objective of this study was to identify the most reliable approach for prevalence estimation ofMycobacterium aviumssp.paratuberculosis(MAP) infection in clinically healthy slaughtered cattle. Sampling of macroscopically suspect tissue was compared to systematic sampling. Specimens of ileum, jejunum, mesenteric and caecal lymph nodes were examined for MAP infection using bacterial microscopy, culture, histopathology and immunohistochemistry. MAP was found most frequently in caecal lymph nodes, but sampling more tissues optimized the detection rate. Examination by culture was most efficient while combination with histopathology increased the detection rate slightly. MAP was detected in 49/50 animals with macroscopic lesions representing 1·35% of the slaughtered cattle examined. Of 150 systematically sampled macroscopically non-suspect cows, 28·7% were infected with MAP. This indicates that the majority of MAP-positive cattle are slaughtered without evidence of macroscopic lesions and before clinical signs occur. For reliable prevalence estimation of MAP infection in slaughtered cattle, systematic random sampling is essential.

scholarly journals CAVEAT ON THE ERROR ANALYSIS FOR STEREOLOGICAL ESTIMATES

2011 ◽  
Vol 19 (1) ◽  
pp. 9 ◽  
Author(s):  
Zhengwei Yang ◽  
Rendong Zhang ◽  
Xiaohong Wen ◽  
Anpei Huang
Keyword(s):  
Error Analysis ◽  
Random Sampling ◽  
Total Error ◽  
Point Of View ◽  
Systematic Sampling ◽  
Systematic Random Sampling ◽  
Systematic Sample ◽  
Pros And Cons ◽  
Animal Organ ◽  
The Difference

It is frequently asked that how big a sample size, or how much measurement, is needed to achieve an accuratestereological estimate. The observed total error of a stereological estimate arises from individual difference (i.e. i ter-animal / organ difference or biological variation) and intra-individual variation (or the stereological error). Statistical methods for error analysis familiar to most biological researchers are based on independent random sampling, however systematic random sampling, which is usually more efficient, is almost always performed in practice. A number of methods for error analysis were utilized in a number of model and actual studies in this paper to demonstrate from a practical point of view the pros and cons of different error analytical methods. Assumption of independence for a systematic sampling will result in overestimation of the stereological error as shown by the studies. A simple and practical approach for error analysis as recommended in this paper is to divide the systematic sample from an organ into two systematic sub-samples, regard them as two independent sub-samples and then compare the difference between the two sub-sample means.

scholarly journals Use of Pooled Fecal Culture for Sensitive and Economic Detection of Mycobacterium avium subsp. paratuberculosisInfection in Flocks of Sheep

2000 ◽  
Vol 38 (7) ◽  
pp. 2550-2556 ◽  
Author(s):  
R. J. Whittington ◽  
S. Fell ◽  
D. Walker ◽  
S. McAllister ◽  
I. Marsh ◽  
...  
Keyword(s):  
Computer Simulation ◽  
Mycobacterium Avium ◽  
Random Sampling ◽  
Clinical Signs ◽  
Control Program ◽  
Pilot Scale ◽  
Fecal Culture ◽  
Sample Collection ◽  
Laboratory Cost ◽  
Fecal Samples

Ovine Johne's disease, or paratuberculosis, occurs in many countries. In Australia, surveillance using serology is used as part of a control program, but the testing regime is costly relative to its sensitivity. For this reason, culturing of Mycobacterium avium subsp. paratuberculosis in fecal samples pooled from a number of sheep was evaluated. Initially, the effect of pooling on the sensitivity of fecal culture was evaluated using samples from 20 sheep with multibacillary paratuberculosis and 20 sheep with paucibacillary paratuberculosis, each confirmed histologically. All multibacillary cases and 50% of paucibacillary cases were detected by culturing of feces at a pooling rate of 1 infected plus 49 uninfected sheep. In a pilot-scale study in 1997,M. avium subsp. paratuberculosis was detected by pooled fecal culture on 93% of 27 infected farms which were identified originally based on history, clinical signs, and one or more rounds of testing using serologic and histopathologic examinations. Pooled fecal culture was compared with serologic examination for submissions from 335 farms where both tests had been conducted on the same sheep and was significantly more sensitive (P < 0.001). Computer simulation of random sampling indicated that the testing of 6 pools of 50 sheep would provide 95% confidence in detecting ≥2% prevalence of infection. The estimated laboratory cost of pooled fecal culture when applied as a flock test is approximately 30% that of serologic examination, and sample collection costs are lower. It is recommended that pooled fecal culture replace serologic examination for detection of M. avium subsp.paratuberculosis infection at the flock level.

scholarly journals On the Estimation of Population Mean Under Systematic Sampling Using Auxiliary Attributes

2016 ◽  
Vol 1 (1-2) ◽  
pp. 21-25 ◽  
Author(s):  
Usman Shahzad
Keyword(s):  
Mean Square Error ◽  
Random Sampling ◽  
Simple Random Sampling ◽  
Sampling Scheme ◽  
Systematic Sampling ◽  
Mean Square ◽  
Numerical Illustration ◽  
Systematic Random Sampling ◽  
Population Mean ◽  
New Family

Naik and Gupta (1996), Singh et al. (2007) and Abd-Elfattah et al. (2010) introduced some estimators for estimating population mean using available auxiliary attributes under simple random sampling scheme. We adapt these estimators under systematic random sampling scheme using available auxiliary attributes. Further, a new family of estimators is proposed for the estimation of population mean under systematic random sampling scheme. The properties such as bias and mean square error of the proposed estimators are derived. From numerical illustration it is shown that proposed estimators are more efficient than the reviewed ones.

HUBUNGAN ANTARA UMUR DAN STATUS PERKAWINAN DENGAN KEJADIAN KANKER PAYUDARA DI INSTALASI BEDAH RUMAH SAKIT UMUM PUSAT DR. MOHAMMAD HOESIN PALEMBANG TAHUN 2012

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Rini Mayasari Rini Mayasari
Keyword(s):  
Breast Cancer ◽  
Marital Status ◽  
Cancer Incidence ◽  
Random Sampling ◽  
Breast Cancer Incidence ◽  
P Value ◽  
Chi Square ◽  
Cross Sectional ◽  
Systematic Random Sampling ◽  
Breast Cancer Event

ABSTRACT Breast cancer on the mark with the growth or uncontrolled growth of cells that are excessive. Factors affecting the incidence of breast cancer include maternal age, marital status, family history, hormone use, radiation, manarche, and obesity. The purpose of this study was to determine the relationship between Age and Marital Status of Mother with Breast Cancer incidence in the Installation Surgeon General Hospital Dr. Mohammad Hoesin Palembang in 2012. The study design was analytical descriptive with the approach in which the independent variables Cross Sectional Age Mother and Status Perkawianan and dependent variables in breast cancer incidence collected at the same time. The study population was all women who develop breast cancer in the Installation General Hospital Surgery Center Dr. Mohammad Hoesin Palembang in 2012 and samples taken by systematic random sampling with a sample of 130 respondents. The results of univariate analysis of data showed that respondents had breast cancer with a percentage of total 58 (44.61%) while respondents who had not had breast cancer totaled 72 by the percentage (63.39%). Respondents by Age Mothers who are at risk amounted to 34 by the percentage (26.15%) while respondents with Age Mothers who are not at risk amounted to 96 by the percentage (73.85%). Respondents by Marital Status is married and the mother who totaled 69 by the percentage (53.07%) and Marital Status with the status of unmarried women totaled 61 by the percentage (46.03%). Of Chi-Square test results found no significant relationship between Age Mothers with Breast Cancer event in which the p value = 0.007, no significant relationship between Marital Status in Breast Cancer event in which the p value = 0.030. Based on the results of the study, researchers suggest counseling efforts, medical knowledge and improve the quality of health services in order to reduce the incidence of breast cancer..   ABSTRAK Kanker payudara di tandai dengan pertumbuhan atau perkembangan tidak terkontrol dari sel-sel yang berlebihan. Faktor-faktor yang mempengaruhi kejadian kanker payudara antara lain umur ibu, status perkawinan, riwayat keluarga, penggunaan hormon, radiasi, manarche, dan obesitas. Tujuan penelitian ini adalah untuk mengetahui hubungan antara Umur Ibu dan Status Perkawinan dengan kejadian Kanker Payudara di Instalasi Bedah Rumah Sakit Umum Pusat Dr. Mohammad Hoesin Palembang Tahun 2012. Desain Penelitian ini adalah deskriptif analitik dengan pendekatan Cross Sectional dimana variabel independen Umur Ibu dan Status Perkawianan dan variabel dependen kejadian kanker payudara dikumpulkan dalam waktu yang bersamaan. Populasi penelitian ini adalah semua ibu yang menderita kanker payudara di Instalasi Bedah Rumah Sakit Umum Pusat Dr. Mohammad Hoesin Palembang Tahun 2012 dan sampel di ambil secara systematic random sampling dengan jumlah sampel 130 responden. Hasil analisis univariat data menunjukan responden yang mengalami Kanker Payudara berjumlah 58 dengan persentase sebesar (44,61%) sedangkan responden yang tidak mengalami Kanker Payudara berjumlah 72 dengan persentase (63,39%). Responden dengan Umur Ibu yang beresiko berjumlah 34 dengan persentase (26,15%) sedangkan responden dengan Umur Ibu yang tidak beresiko berjumlah 96 dengan persentase (73,85%). Responden dengan Status Perkawinan ibu yang berstatus kawin berjumlah 69 dengan persentase (53,07%) dan Status Perkawinan ibu yang berstatus tidak kawin berjumlah 61 dengan persentase (46,03%). Dari hasil uji Chi-Square didapatkan ada hubungan bermakna antara Umur Ibu dengan kejadian Kanker Payudara dimana p Value = 0,007, ada hubungan bermakna antara Status Perkawinan dengan kejadian Kanker Payudara dimana p Value = 0,030. Berdasarkan hasil penelitian, peneliti menyarankan upaya-upaya penyuluhan, pengetahuan tenaga medis dan meningkatkan mutu pelayanan kesehatan guna menurunkan angka kejadian kanker payudara.

HUBUNGAN UMUR DAN PARITAS IBU DENGAN KEJADIAN HIPEREMESIS GRAVIDARUM DI RUMAH SAKIT UMUM PUSAT Dr. MOHAMMAD HOESIN PALEMBANG TAHUN 2011

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Wahyu Ida Muliana Wahyu Ida Muliana
Keyword(s):  
Pregnant Women ◽  
Medical Record ◽  
Random Sampling ◽  
Hyperemesis Gravidarum ◽  
P Value ◽  
Chi Square ◽  
Cross Sectional ◽  
Systematic Random Sampling ◽  
Pregnancy And Childbirth ◽  
Chi Square Test

ABSTRACT Hyperemesis Gravidarum marked excessive nausea and vomiting in pregnant women at a young age. WHO estimates that 536,000 women died from direct complications of pregnancy and childbirth. One complication of pregnancy is Hyperemesis Gravidarum. Hyperemesis Gravidarum in the world has been estimated to occur in 1-2% of pregnant. According to data from the Medical Record of Dr. Mohammad Hoesin Palembang Hospital, the incidence of Hyperemesis Gravidarum in 2011 there were 72 people of 661 pregnant women. The purpose of this reseach was to determine the relationship between maternal age and parity with Hyperemesis Gravidarum in Dr. Mohammad Hoesin Palembang Hospital 2011. The Design of this reseach used Cross Sectional by analytic approach survey. The population of this reseach are all of mother who gestational ≤ 16 weeks (four months) in the Installation of Obstetrics and Gynecology, Dr. Mohammad Hoesin Palembang Hospital in January to December of 2011 with the sample of 661 people which taken by systematic random sampling and the reseach was conducted from 17 April to 24 April 2012.  Each variable that was observed in tests using Chi-Square test with a (0.05).  The results of this study showed that 5.9% of mothers with hyperemesis gravidarum, 21.8% of mothers with high risk age, and 30.0% primigravida. Chi-Square test showed no significant relationship between age (p value = 0.000) and parity (p value = 0.000) with the incidence of hyperemesis gravidarum. Expected to the Hospital to be implemented properly instructed how to provide counseling to pregnant women about pregnancy and childbirth, as well as provide confidence that the nausea and vomiting is a symptom of physiology in pregnancy.   ABSTRAK Hiperemesis Gravidarum ditandai mual dan muntah yang berlebihan terjadi pada ibu hamil di usia muda. WHO memperkirakan 536.000 perempuan meninggal dunia akibat langsung dari komplikasi kehamilan dan persalinan. Salah satu komplikasi kehamilan adalah Hiperemesis Gravidarum. Insiden Hiperemesis Gravidarum di dunia telah diperkirakan terjadi pada 1-2% wanita hamil. Menurut data dari Medical Record Rumah Sakit Umum Pusat Dr. Mohammad Hoesin Palembang, angka kejadian Hiperemesis Gravidarum pada tahun 2011 terdapat 72 orang dari 661 ibu hamil. Tujuan penelitian ini adalah untuk mengetahui hubungan antara umur dan paritas ibu dengan kejadian Hiperemesis Gravidarum pada ibu di Rumah Sakit Umum Pusat Dr. Mohammad Hoesin Palembang tahun 2011. Desain Penelitian ini menggunakan survei analitik dengan pendekatan Cross Sectional. Populasi penelitian ini adalah dengan umur kehamilan ≤ 16 minggu (4 bulan) yang pernah dirawat inap di Instalasi Kebidanan dan Penyakit Kandungan Rumah Sakit Umum Pusat Dr. Mohammad Hoesin Palembang pada bulan Januari-Desember tahun 2011 dengan jumlah sample 661 orang yang diambil secara systematic Random Sampling (secara acak sistematis) dan penelitian ini dilakukan dari tanggal 17 April sampai dengan 24 April 2012. Masing-masing variabel yang diteliti di uji dengan menggunakan uji Chi-Square dengan a (0,05). Hasil penelitian ini menunjukkan bahwa 5,9% ibu mengalami hiperemesis gravidarum, 21,8% ibu dengan umur resiko tinggi, dan 30,0% ibu primigravida. Uji Chi-Square menunjukkan ada hubungan yang bermakna antara umur (p value = 0,000) dan paritas (p value = 0,000) dengan kejadian hiperemesis gravidarum. Diharapkan dapat menjadi masukan bagi pihak Rumah Sakit agar dilaksanakan penyuluhan dengan cara memberikan konseling terhadap ibu hamil tentang kehamilan dan persalinan, serta memberikan keyakinan bahwa mual muntah merupakan gejala fisiologi pada kehamilan.

SKRINING MALARIA DENGAN RAPID DIAGNOSTIC TEST DAN PERILAKU PENGOBATAN MALARIA PADA ORANG RIMBA DI DESA BUKIT SUBAN DAN DESA SEKAMIS KABUPATEN SAROLANGUN TAHUN 2016

2018 ◽  
Vol 6 (1) ◽  
pp. 20-35
Author(s):  
Maria Estela Karolina ◽  
Oktovia Rezka Nurmaajid ◽  
Armaidi Darmawan ◽  
Solha Elfrida
Keyword(s):  
Traditional Medicine ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Random Sampling ◽  
Descriptive Study ◽  
Malaria Treatment ◽  
Systematic Random Sampling

Abstract Backgrounds : Rapid Diagnostic Test (RDT) is a imunologic methode to diagnose malaria. Mostly, Orang Rimba hunt nomadically. Orang Rimba have traditional medicine to cure malaria. The purposes of this research are to know the incident of malaria based on RDT and malaria treatment behaviour of Orang Rimba. Methode :  This research was descriptive study. The populations of this research were Orang Rimba in Desa Bukit Suban and Sekamis, Kabupaten Sarolangun. The number of samples in this research is 49 respondents. The sample was taken by systematic random sampling. Data were analyzed with univariat analysis. Result : The result showed that 16,7 % had malaria positive, P. vivax was the dominat species of plasmodium (62,5%). Mostly the aged of respondent was 5-11 years old (41,7%), the gender was male (58,3%), occupation was doesn’t work (56,3%), marriage status was marriage (56,3%). Most of Orang Rimba had worse knowladge level (53,6%), the mostly used term in Orang Rimba was demam kuro (50%), only (42,9%) respondents knew the trias of malaria, only 9 respondents knew that cause of malaria was mosquito’s bites, Orang Rimba mostly knew the danger of malaria (85,7%) and the complication of malaria (89,3%), and knew that malaria could be cured (82,1%). The users of modern combined with traditional medicine were as much as 57,1%, Orang Rimba mostly used ≥ 3 kinds of traditional medicine 53,6%, traditional medicine was mostly processed by boiling (24 respondents) and mostly knew one way of processing the traditional medicine  (57,1%), traditional medicine was mostly for being eaten or drunk and external medicine  (50%), the duration of using  traditional medicine was mostly  2-3 days (46,4%), Orang Rimba mostly said that traditional medicine was efficacious (92,9%). Conclusions : The incident number of malaria was 16,7% and the mostly users of modern combined with traditional medicine were as much as 57,1%. Keywords : Malaria, RDT, orang rimba, malaria treatment behaviour   Abstrak Latar Belakang : Rapid Diagnostic Test (RDT) merupakan metoda imunologik untuk mendiagnosis malaria. Lokasi berburu Orang Rimba cenderung berpindah-pindah. Orang Rimba memiliki kearifan lokal dalam mengobati malaria. Tujuan penelitian ini adalah untuk mengetahui skrining malaria berdasarkan RDT dan perilaku pengobatan malaria pada Orang Rimba. Metode : Penelitian ini merupakan penelitian deskriptif. Populasi penelitian seluruh Orang Rimba yang berada di Desa Bukit Suban dan Sekamis. Jumlah sampel pada penelitian ini adalah 48 responden. Pengambilan sampel menggunakan teknik Systematic Random Sampling. Data dianalisis dengan analisis univariat. Hasil : Dari hasil penelitian didapatkan 16,7% positif malaria, jenis plasmodium yang dominan adalah P. vivax (62,5%). Sebagian besar responden berusia 5-11 tahun (41,7%), sebagian besar berjenis kelamin laki-laki (58,3%), sebagian besar pekerjaan responden tidak bekerja (56,3%), sebagian besar berstatus kawin (56,3%). Sebagian besar Orang Rimba memiliki tingkat pengetahuan kurang baik (53,6%), sebagian besar menggunakan istilah demam kuro untuk menyebutkan malaria (50%), hanya 42,9% yang mengetahui trias malaria, hanya 9 responden yang menjawab penyebab malaria adalah gigitan nyamuk, sebagian besar mengetahui bahaya malaria 85,7%, jenis bahaya malaria 89,3%, dan mengetahui bahwa malaria dapat disembuhkan 82,1%. Pengguna pengobatan modern dikombinasi dengan pengobatan tradisional sebanyak 57,1%, sebagian besar menggunakan ≥ 3 jenis obat tradisional (53,6%), cara pengolahan yang paling banyak dengan cara direbus (24 responden) dan mengetahui 1 cara pengolahan (57,1%), cara pemakaian yang paling banyak dengan dimakan atau diminum dan obat luar (50%), lama penggunaan yang paling banyak selama 2-3 hari (46,4%), sebagian besar mengatakan obat tradisional berkhasiat (92,9%). Kesimpulan : Angka kejadian malaria sebesar 16,7% dan upaya pengobatan malaria terbanyak adalah dengan pengobatan modern dikombinasi dengan pengobatan tradisional (57,1%).   Kata Kunci :  Malaria, RDT, orang rimba, perilaku pengobatan malaria

scholarly journals Comparison of Variance Estimators for Systematic Environmental Sample Surveys: Considerations for Post-Stratified Estimation

Forests ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 772
Author(s):  
Bryce Frank ◽  
Vicente J. Monleon
Keyword(s):  
Random Sampling ◽  
Simple Random Sampling ◽  
National Forest Inventory ◽  
Point Estimation ◽  
Systematic Sampling ◽  
Sampling Variance ◽  
Variance Estimators ◽  
The Past ◽  
Point Estimators ◽  
Species Specific

The estimation of the sampling variance of point estimators under two-dimensional systematic sampling designs remains a challenge, and several alternative variance estimators have been proposed in the past few decades. In this work, we compared six alternative variance estimators under Horvitz-Thompson (HT) and post-stratification (PS) point estimation regimes. We subsampled a multitude of species-specific forest attributes from a large, spatially balanced national forest inventory to compare the variance estimators. A variance estimator that assumes a simple random sampling design exhibited positive relative bias under both HT and PS point estimation regimes ranging between 1.23 to 1.88 and 1.11 to 1.78 for HT and PS, respectively. Alternative estimators reduced this positive bias with relative biases ranging between 1.01 to 1.66 and 0.90 to 1.64 for HT and PS, respectively. The alternative estimators generally obtained improved efficiencies under both HT and PS, with relative efficiency values ranging between 0.68 to 1.28 and 0.68 to 1.39, respectively. We identified two estimators as promising alternatives that provide clear improvements over the simple random sampling estimator for a wide variety of attributes and under HT and PS estimation regimes.

scholarly journals Clinical Significance of ROMs, OXY, SHp and HMGB-1 in Canine Leishmaniosis

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 754
Author(s):  
Michela Pugliese ◽  
Alessandra Sfacteria ◽  
Gaetano Oliva ◽  
Annastella Falcone ◽  
Manuela Gizzarelli ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lymph Nodes ◽  
Clinical Signs ◽  
Biological Tissues ◽  
Stress Index ◽  
Control Group ◽  
Canine Leishmaniosis ◽  
Oxidative Stress Parameters ◽  
Stress Parameters

This study aimed to investigate the role of oxidative stress parameters (ROMs, OXY, SHp), the Oxidative Stress index (OSi), and High Mobility Group Box-1 protein (HMGB-1) in canine leishmaniosis (CanL). For this study, thirty dogs, naturally infected with Leishmania spp. (Leishmania Group, LEISH) and ten healthy adult dogs (control group, CTR) were included. The diagnosis of CanL was performed by a cytological examination of lymph nodes, real time polymerase chain reaction on biological tissues (lymph nodes and whole blood), and an immunofluorescence antibody test (IFAT) for the detection of anti-Leishmania antibodies associated with clinical signs such as dermatitis, lymphadenopathy, onychogryphosis, weight loss, cachexia, lameness, conjunctivitis, epistaxis, and hepatosplenomegaly. The HMGB-1 and oxidative stress parameters of the LEISH Group were compared with the values recorded in the CTR group (Mann Whitney Test, p < 0.05). Spearman rank correlation was applied to evaluate the correlation between the HMGB-1, oxidative stress biomarkers, hematological and biochemical parameters in the LEISH Group. Results showed statistically significant higher values of SHp in the LEISH Group. Specific correlation between the ROMs and the number of red blood cells, and between HGMB-1 and SHp were recorded. These preliminary data may suggest the potential role of oxidative stress in the pathogenesis of CanL. Further studies are undoubtedly required to evaluate the direct correlation between inflammation parameters with the different stages of CanL. Similarly, further research should investigate the role of ROMs in the onset of anemia.

Mycobacterium avium subsp. avium in Lymph Nodes and Diaphragms of Pigs from One Infected Herd in the Czech Republic

2014 ◽  
Vol 77 (1) ◽  
pp. 141-144 ◽  
Author(s):  
PETR KRIZ ◽  
MARIJA KAEVSKA ◽  
IVA SLANA ◽  
IVA BARTEJSOVA ◽  
IVO PAVLIK
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Avium ◽  
Mycobacterium Avium Subsp ◽  
Mesenteric Lymph Nodes ◽  
Tissue Samples ◽  
The Czech Republic ◽  
Mesenteric Lymph ◽  
Qpcr Method ◽  
Pig Carcasses ◽  
Slaughtered Pigs

This study was performed on 40 finished pigs from one herd naturally infected with Mycobacterium avium subsp. avium. The aim was to investigate the presence and amount of M. a. avium in samples of lymph nodes and diaphragm tissues collected during routine postmortem inspection using the triplex quantitative real time PCR (qPCR) method. We collected, in total, 107 samples: various lymph nodes affected by gross tuberculosis (TB)–like lesions from 17 pig carcasses, as well as samples of head and mesenteric lymph nodes from 23 carcasses without TB-like lesions. Samples of diaphragm tissues were collected from all carcasses. M. a. avium was detected in one or more tissue samples collected from half of the slaughtered pigs tested. Samples of diaphragm tissues of three pigs with detected TB-like lesions contained M. a. avium (102 to 103 cells per g of sample); the organism was not detected in diaphragm tissues from pigs without TB-like lesions. The qPCR method may be useful for quantification of M. a. avium in pigs for the purposes of foodborne risk assessment.

scholarly journals Pathology and Pathophysiology of Inhalational Anthrax in a Guinea Pig Model

2013 ◽  
Vol 81 (4) ◽  
pp. 1152-1163 ◽  
Author(s):  
Vladimir Savransky ◽  
Daniel C. Sanford ◽  
Emily Syar ◽  
Jamie L. Austin ◽  
Kevin P. Tordoff ◽  
...  
Keyword(s):  
Animal Model ◽  
Guinea Pig ◽  
Lymph Nodes ◽  
Guinea Pigs ◽  
Alternative Model ◽  
Lethal Dose ◽  
Clinical Signs ◽  
Regional Lymph Nodes ◽  
Content Type ◽  
Ames Strain

ABSTRACTNonhuman primates (NHPs) and rabbits are the animal models most commonly used to evaluate the efficacy of medical countermeasures against anthrax in support of licensure under the FDA's “Animal Rule.” However, a need for an alternative animal model may arise in certain cases. The development of such an alternative model requires a thorough understanding of the course and manifestation of experimental anthrax disease induced under controlled conditions in the proposed animal species. The guinea pig, which has been used extensively for anthrax pathogenesis studies and anthrax vaccine potency testing, is a good candidate for such an alternative model. This study was aimed at determining the median lethal dose (LD50) of theBacillus anthracisAmes strain in guinea pigs and investigating the natural history, pathophysiology, and pathology of inhalational anthrax in this animal model following nose-only aerosol exposure. The inhaled LD50of aerosolized Ames strain spores in guinea pigs was determined to be 5.0 × 104spores. Aerosol challenge of guinea pigs resulted in inhalational anthrax with death occurring between 46 and 71 h postchallenge. The first clinical signs appeared as early as 36 h postchallenge. Cardiovascular function declined starting at 20 h postexposure. Hematogenous dissemination of bacteria was observed microscopically in multiple organs and tissues as early as 24 h postchallenge. Other histopathologic findings typical of disseminated anthrax included suppurative (heterophilic) inflammation, edema, fibrin, necrosis, and/or hemorrhage in the spleen, lungs, and regional lymph nodes and lymphocyte depletion and/or lymphocytolysis in the spleen and lymph nodes. This study demonstrated that the course of inhalational anthrax disease and the resulting pathology in guinea pigs are similar to those seen in rabbits and NHPs, as well as in humans.

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