Symbiotic efficiency of pigeonpea (Cajanus cajan) with different sources of nitrogen

2021 ◽  
pp. 1-4
Author(s):  
Francina L. Bopape ◽  
Eastonce T. Gwata ◽  
Ahmed I. Hassen ◽  
Marvellous M. Zhou
Keyword(s):  
Dry Weight ◽  
Soil Samples ◽  
Grain Legume ◽  
N Fixation ◽  
Symbiotic Efficiency ◽  
Native Soil ◽  
Rhizobial Isolate ◽  
Production Area ◽  
Biological Nitrogen ◽  
Different Sources

Abstract Pigeonpea is an important grain legume. It contributes to the improvement of soil fertility through biological nitrogen (N) fixation. However, the symbiotic efficiency of pigeonpea with native soil rhizobia has not been determined adequately. This study was designed to determine the variation in the N fixation ability of pigeonpea inoculated with the native rhizobia. Forty soil samples were collected from diverse locations across South Africa and used for inoculating pigeonpea seed. Each pigeonpea genotype was inoculated separately with each soil sample and raised in a nitrogen-depleted growth medium in the greenhouse. A split-plot experimental design was used in the study. Several N fixation variables of pigeonpea were measured. There was >40.0% difference in the number of nodules between genotypes ‘Ex-PP-MD-321’ and ‘Mpuma-B-Spot’ but the nodule dry weight between the two genotypes was >80.0%. In contrast, the heaviest dry shoots (0.4513 g), weighed 52.0% heavier than those that were observed for ‘Mpuma-B-Spot’. Pigeonpea showed differential N fixation ability with the nodules, suggesting that there was potential to select for optimum host × rhizobial isolate combinations for the process and to expand the production area of the crop.

scholarly journals Symbiotic capability of calopo rhizobia from an agrisoil with different crops in Pernambuco

2013 ◽  
Vol 37 (4) ◽  
pp. 869-876 ◽  
Author(s):  
Altanys Silva Calheiros ◽  
Mario de Andrade Lira Junior ◽  
Débora Magalhães Soares ◽  
Márcia do Vale Barreto Figueiredo
Keyword(s):  
Dry Matter ◽  
Nitrogen Sources ◽  
Dry Weight ◽  
Dry Forest ◽  
Forest Zone ◽  
Symbiotic Efficiency ◽  
Total N ◽  
Calopogonium Mucunoides ◽  
Legume Symbiosis ◽  
Biological Nitrogen

Biological nitrogen fixation by rhizobium-legume symbiosis represents one of the most important nitrogen sources for plants and depends strongly on the symbiotic efficiency of the rhizobium strain. This study evaluated the symbiotic capacity of rhizobial isolates from calopo (CALOPOGONIUM MUCUNOIDES) taken from an agrisoil under BRACHIARIA DECUMBENS pasture, sabiá (MIMOSA CAESALPINIIFOLIA) plantations and Atlantic Forest areas of the Dry Forest Zone of Pernambuco. A total of 1,575 isolates were obtained from 398 groups. A single random isolate of each group was authenticated, in randomized blocks with two replications. Each plant was inoculated with 1 mL of a bacterial broth, containing an estimated population of 10(8) rhizobial cells mL-1. Forty-five days after inoculation, the plants were harvested, separated into shoots, roots and nodules, oven-dried to constant mass, and weighed. Next, the symbiotic capability was tested with 1.5 kg of an autoclaved sand:vermiculite (1:1) mixture in polyethylene bags. The treatments consisted of 122 authenticated isolates, selected based on the shoot dry matter, five uninoculated controls (treated with 0, 50, 100, 150, or 200 kg ha-1 N) and a control inoculated with SEMIA 6152 (=BR1602), a strain of BRADYRHIZOBIUM JAPONICUM The test was performed as described above. The shoot dry matter of the plants inoculated with the most effective isolates did not differ from that of plants treated with 150 kg ha-1 N. Shoot dry matter was positively correlated with all other variables. The proportion of effective isolates was highest among isolates from SABIÁ forests. There was great variation in nodule dry weight, as well as in N contents and total N.

scholarly journals Differential Response of Cowpea (Vigna unguiculata L.) Genotypes to Native Rhizobia in Tarai Region of Uttarakhand, India

2014 ◽  
Vol 6 (3) ◽  
pp. 335-337
Author(s):  
Sonam BINJOLA ◽  
Narendra KUMAR ◽  
Gaurav MISHRA
Keyword(s):  
Developing Countries ◽  
Grain Yield ◽  
Protein Content ◽  
Vigna Unguiculata ◽  
Growth Analysis ◽  
Critical Role ◽  
Dry Weight ◽  
Differential Response ◽  
Symbiotic Efficiency ◽  
Native Soil

Cowpea is an annual legume, rich in protein, which plays a critical role in the lives of millions of people in developing countries. Limited work has been done, especially on the symbiotic efficiency of cowpea against the native soil rhizobia. Therefore, our study was conducted to compare the effect of native rhizobia on nodulation, yield and protein content of eight cultivars and two control varieties in Tarai region of Uttarakhand, India. significant differences were observed among the cultivars tested in all the measured traits. Results of growth analysis indicated that ‘PGCP-4’ gave the highest number of nodules plant-1, nodule dry weight and protein content in grain, compared to both control varieties. Highest plant dry weight was recorded in ‘Pusa Komal’, while the highest grain yield was achieved in ‘PGCP-6’, followed by ‘PGCP-12’. This study hereby recommends ‘PGCP-6’ and ‘PGCP-12’ cultivars, as they are more suitable in terms of yield against the rhizobia.

scholarly journals Does inoculation with native rhizobia enhance nitrogen fixation and yield of cowpea through legume-based intercropping in the northern mountainous areas of Vietnam?

2020 ◽  
pp. 1-12
Author(s):  
Trung Thanh Nguyen ◽  
Mary Atieno ◽  
Laetitia Herrmann ◽  
Sutkhet Nakasathien ◽  
Ed Sarobol ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Dry Weight ◽  
Mountainous Region ◽  
Symbiotic Efficiency ◽  
N Application ◽  
Shoot Dry Weight ◽  
Uninoculated Control ◽  
Adoption Level ◽  
Biological Nitrogen ◽  
Local Farmers

Abstract In the Northern mountainous region of Vietnam, cassava–cowpea intercropping system has been widely promoted with support from the local agricultural department. However, cowpea yield is often limited because of a low Biological Nitrogen Fixation (BNF) activity due to its low natural nodulation and lack of available effective Rhizobium products. The aim of this study was to identify the most effective native rhizobia isolate nodulating cowpea with the potential to increase BNF and yield of cowpea. A greenhouse experiment was initially conducted with five treatments: three native rhizobia isolates (CMBP037, CMBP054, and CMBP065); a control (no inoculation and no N application); and N+ (no inoculation, application of N as KNO3). Field inoculations were carried out and the treatments were as follows: a control (no inoculation); CMBP (037+054) – a mixture of strains from Mau Dong; CMBP065 strain from Cat Thinh. CMBP054 and CMBP065 had the highest nodulation in the greenhouse (46.4 and 60.7 nodules plant−1, respectively) and were rated as effective with symbiotic efficiency (SEF) of 54.56 and 55.73%, respectively. In the field, CMBP (037+054) recorded significantly higher nodulation (19.4 nodules plant−1) than the control (11.7 nodules plant−1). CMBP (037+054) also increased cowpea shoot dry weight, shoot N, and yield by 28.6, 4.9, and 10.5%, respectively, compared to the uninoculated control. This effect was slope dependent (statistically significant in moderate and steep slope, not with gentle slope). Besides, the high expansion rate of intercropping with cowpea showed the high adoption level of these agroecological practices by local farmers. This study reveals the potential of native rhizobia inoculation to enhance soil fertility and sustainable agriculture in the Northern mountainous region of Vietnam and proposes enhanced efforts to promote the availability and utilization of effective inoculants for cowpea.

scholarly journals Evaluation of the Symbiotic Efficiency of Rhizobium Strains Applied to Bituminaria bituminosa (L.) Stirton

2020 ◽  
pp. 45-55
Author(s):  
Omar Zennouhi ◽  
Abderrazak Rfaki ◽  
Mohamed El Mderssa ◽  
Jamal Ibijbijen ◽  
Laila Nassiri
Keyword(s):  
Nitrogen Fixation ◽  
Total Nitrogen ◽  
Biological Nitrogen Fixation ◽  
Dry Weight ◽  
Pastoral Systems ◽  
Plant Resources ◽  
Symbiotic Efficiency ◽  
Rhizobium Strains ◽  
Bituminaria Bituminosa ◽  
Biological Nitrogen

Aims: The study aimed to evaluate the effect of inoculation by different rhizobacteria on Bituminaria bituminosa plants grown under greenhouse conditions. Study Design: An experimental study. Place and Duration of Study: The study was carried out at the Department of Biology (Environment and valorization of microbial and plant resources Unit), Faculty of Sciences, Moulay Ismail University-Meknes, from November 2019 to February 2020. Methodology: Eleven species and/or isolates belonging to Rhizobium genus are used to inoculate B. bituminosa plants; similarly, fresh and dry crushed nodules previously collected from B. bituminosa shrubs are tested. The bacterial inoculation effects are evaluated through the estimation of inoculated plants’ fresh and dry shoots weight, root dry weight, total nitrogen, nodules number and fresh weight in comparison to non-inoculated plants. The infectivity and efficiency of the bacteria and the biological nitrogen fixation are also evaluated. Results: The results enable us to select the infective strains on the basis of their positive effect on growth and total nitrogen, in order to produce inoculum for B. bituminosa. Efficiency and biological nitrogen fixation are also very high compared to the control, especially with the B.b1 strain isolated from Bituminaria bituminosa and identified as Rhizobium tibeticum. The fresh nodules crushing is also very efficient. as inoculant. Conclusion: The use of symbiotic complex as Rhizobium tibeticum – Bituminaria bituminosa or an inoculum produced from fresh nodules are an eco-friendly alternative for the design of sylvo-pastoral systems ensuring increased soil fertility, fodder productivity and sustainable agroforestry.

Comparative Effect of Groundnut (Arachis hypogea l.) Genotypes on Yield and N Fixation in Sudan and Northern Guinea Savannahs of Nigeria

2019 ◽  
pp. 61-67
Keyword(s):  
Agricultural Research ◽  
Cropping Systems ◽  
Block Design ◽  
Dry Weight ◽  
Field Trials ◽  
Yield Potential ◽  
N Fixation ◽  
Maize Crop ◽  
Significant Interaction Effect ◽  
Pod Yield

Recognition of high yielding and nitrogen (N) fixing groundnut genotypes and desegregating them in the cereal-based cropping systems common in savannah regions will enhance food security and reduce the need for high N fertilizers hence, minimize the high cost and associated environmental consequences. Field trials were conducted during the 2015 growing season at the Research Farms of Bayero University Kano (BUK) and Institute for Agricultural Research (IAR), Ahmadu Bello University, Samaru-Zaria to assess the yield potential and Biolog- ical N fixation in 15 groundnut genotypes (ICG 4729, ICGV-IS 07823, ICGV-IS 07893, ICGV-IS 07908, ICGV- SM 07539, ICGV- SM 07599, ICGV-IS 09926, ICGV-IS 09932, ICGV-IS 09992, ICGV-IS 09994, SAMNUT-21, SAMNUT-22, SAMNUT-25, KAMPALA and KWANKWAS). The groundnut genotypes and reference Maize crop (SAMMAZ 29) were planted in a randomized complete block design in three replications. N difference method was used to estimate the amount of N fixed. The parameters determined were the number of nodules, nod- ule dry weight, shoot and root dry weights, pod, and haulm yield as well as N fixation. The nodule dry weight, BNF, haulm, and pod yield were statistically significant (P<0.01) concerning genotype and location. Similarly, their interac- tion effect was also highly significant. ICGV-IS 09926 recorded the highest nod- ule dry weight of 2.07mg /plant across the locations while ICGV-IS 09932 had the highest BNF value of 140.27Kg/ha. Additionally, KAMPALA had the high- est haulm yield, while ICGV-IS 07893 had the highest pod yield across the loca- tions with a significant interaction effect. The result shows that ICGV-IS 07893 and ICGV-IS 09932, as well as ICGV-IS 09994 and SAMNUT – 22, were the best genotypes concerning BNF, haulm and pod yield in the Northern Guinea and Sudan Savannahs of Nigeria respectively with the potential for a corresponding beneficial effect.

scholarly journals Mutation in the ntrR Gene, a Member of the vap Gene Family, Increases the Symbiotic Efficiency of Sinorhizobium meliloti

2001 ◽  
Vol 14 (7) ◽  
pp. 887-894 ◽  
Author(s):  
Boglárka Oláh ◽  
Erno Kiss ◽  
Zoltán Györgypál ◽  
Judit Borzi ◽  
Gyöngyi Cinege ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Pathogenic Bacteria ◽  
Sinorhizobium Meliloti ◽  
Root Nodules ◽  
Nifh Gene ◽  
Dry Weight ◽  
Nodule Occupancy ◽  
Gene Encoding ◽  
Symbiotic Efficiency ◽  
Host Interactions

In specific plant organs, namely the root nodules of alfalfa, fixed nitrogen (ammonia) produced by the symbiotic partner Sinorhizobium meliloti supports the growth of the host plant in nitrogen-depleted environment. Here, we report that a derivative of S. meliloti carrying a mutation in the chromosomal ntrR gene induced nodules with enhanced nitrogen fixation capacity, resulting in an increased dry weight and nitrogen content of alfalfa. The efficient nitrogen fixation is a result of the higher expression level of the nifH gene, encoding one of the subunits of the nitrogenase enzyme, and nifA, the transcriptional regulator of the nif operon. The ntrR gene, controlled negatively by its own product and positively by the symbiotic regulator syrM, is expressed in the same zone of nodules as the nif genes. As a result of the nitrogen-tolerant phenotype of the strain, the beneficial effect of the mutation on efficiency is not abolished in the presence of the exogenous nitrogen source. The ntrR mutant is highly competitive in nodule occupancy compared with the wild-type strain. Sequence analysis of the mutant region revealed a new cluster of genes, termed the “ntrPR operon,” which is highly homologous to a group of vap-related genes of various pathogenic bacteria that are presumably implicated in bacterium-host interactions. On the basis of its favorable properties, the strain is a good candidate for future agricultural utilization.

scholarly journals STUDY AND ESTIMATION OF THE RATIO OF 137CS AND 40K SPECIFIC ACTIVITIES IN SANDY AND LOAM SOILS / 137CS IR 40K SAVITŲJŲ AKTYVUMŲ SANTYKIŲ TYRIMAS IR VERTINIMAS PRIESMĖLIO IR PRIEMOLIO DIRVOŽEMYJE

2011 ◽  
Vol 3 (5) ◽  
pp. 37-42
Author(s):  
Renata Mikalauskienė ◽  
Donatas Butkus ◽  
Ingrida Pliopaitė Bataitienė
Keyword(s):  
Chemical Properties ◽  
Dry Weight ◽  
Soil Samples ◽  
Physical And Chemical Properties ◽  
Nuclear Testing ◽  
Specific Activities ◽  
Physical And Chemical ◽  
Chernobyl Nuclear Plant ◽  
Properties Of Soil ◽  
And Chemical Properties

The present article describes changes in specific activities and fluctuations in the ratio of natural 40K and artificial 137Cs radionuclides in soil samples taken from different places of Lithuanian territory. The samples of soil have been selected from the districts polluted after the accident in Chernobyl nuclear plant performing nuclear testing operations. The study has established the main physical and chemical properties of soil samples and their impact on the concentration of 40K activities. 137Cs/40K specific activities in soil have been observed under the dry weight of the sample that varied from 0.0034 to 0.0240. The results of the study could be used for establishing and estimating 137Csand 40K transfer in the system “soil-plant”. Santrauka Straipsnyje nagrinėjama gamtinės (40K) ir dirbtinės (137Cs) kilmės radionuklidų savitųjų aktyvumų ir jų santykio kaita skirtinguose Lietuvos teritorijos dirvožemiuose. Dirvožemio mėginiai parinkti iš vietovių, kurios buvo labiau užterštos po Černobylio atominės elektrinės avarijos ir buvusių branduolinių bandymų. Tyrimo metu nustatytos pagrindinės fizinės cheminės dirvožemio savybės ir jų poveikis 40K aktyvumų koncentracijai. 137Csir 40K savitieji aktyvumai dirvožemyje tirti esant sausam mėginio svoriui. 137Cs savitieji aktyvumai sausame dirvožemyje svyravo nuo 1,1±1,0 iki 14,3±0,9 Bq/kg, o 40K savitieji aktyvumai – nuo 326±29 iki 740±15 Bq/kg. 137Csir 40K savitųjų aktyvumų santykis skirtingų vietovių dirvožemiuose kito nuo 0,0034 iki 0,0240 Bq/kg. Tyrimo rezultatai gali būti panaudoti, nustatant ir įvertinant 137Csir 40K pernašą sistemoje dirvožemis–augalai.

Seed size influences the promoting activity of rhizobia on plant growth, nodulation and N fixation in lima bean

2021 ◽  
Vol 51 (3) ◽  
Author(s):  
Claudyanne do Nascimento Costa ◽  
Jadson Emanuel Lopes Antunes ◽  
João Pedro Alves de Aquino ◽  
Ingrid Sthephanie da Costa Silva ◽  
Angela Celis de Almeida Lopes ◽  
...  
Keyword(s):  
Plant Growth ◽  
Seed Size ◽  
Reference Strain ◽  
Dry Weight ◽  
Lima Bean ◽  
N Fixation ◽  
Nodule Number ◽  
Root Dry Weight ◽  
Biological N Fixation

ABSTRACT: This study evaluated the activity of rhizobia isolates inoculated in large (18 mm) and small (11 mm) seeds on lima bean growth, nodulation and N fixation. Selected rhizobia isolates were compared with a reference strain CIAT899 and two controls without inoculation. Large seeds contributed for highest plant growth, nodulation and N fixation than small seeds. The isolates UFPI-59, UFPI-18 and UFPI-38 promoted the highest values of shoot and root dry weight, respectively. The isolates UFPI-32 promoted the highest values of nodule number, while UFPI-59 promoted the highest values of nodule dry weight. The isolates UFPI-38 and UFPI-59 promoted the highest accumulation of N. This study showed that seed size really influences lima bean growth, nodulation and BNF. Considering rhizobia isolates, UFPI-59, UFPI-38, and UFPI-18 contributed for plant growth, promoted better nodulation and effectiveness on biological N fixation.

scholarly journals Feed legumes for truly sustainable crop-animal systems

2017 ◽  
Vol 12 (2) ◽  
Author(s):  
Paolo Annicchiarico
Keyword(s):  
Unit Area ◽  
Research Effort ◽  
Grain Legumes ◽  
Protein Yield ◽  
White Lupin ◽  
Grain Legume ◽  
N Fixation ◽  
Genetic Progress ◽  
High Feed ◽  
Feed Protein

Legume cultivation has sharply decreased in Italy during the last 50 years. Lucerne remains widely grown (with about 12% of its area devoted to dehydration), whereas soybean is definitely the most-grown grain legume. Poor legume cropping is mainly due to the gap in yielding ability with major cereals, which has widened up in time according to statistical data. Lucerne displays definitely higher crude protein yield and somewhat lower economic gap with benchmark cereals than feed grain legumes. Pea because of high feed energy production per unit area and rate of genetic progress, and white lupin because of high protein yield per unit area, are particularly interesting for Italian rain-fed environments. Greater legume cultivation in Europe is urged by the need for reducing energy and green-house gas emissions and excessive and unbalanced global N flows through greater symbiotic N fixation and more integrated crop-animal production, as well as to cope with ongoing and perspective raising prices of feed proteins and N fertilisers and insecurity of feed protein supplies. The transition towards greater legume cultivation requires focused research effort, comprehensive stakeholder cooperation and fair economic compensation for legume environmental services, with a key role for genetic improvement dragged by public breeding or pre-breeding. New opportunities for yield improvement arise from the ongoing development of cost-efficient genome-enabled selection procedures, enhanced adaptation to specific cropping conditions via ecophysiological and evolutionary-based approaches, and more thorough exploitation of global genetic resources.

scholarly journals Effect of soil interacting herbicides on soybean nodulation in Balcarce, Argentina

1999 ◽  
Vol 34 (7) ◽  
pp. 1167-1173 ◽  
Author(s):  
Norma Gonzalez ◽  
Juan Jose Eyherabide ◽  
Maria Ignacia Barcelonna ◽  
Alfredo Gaspari ◽  
Silvina Sanmartino
Keyword(s):  
Organic Matter ◽  
Dry Weight ◽  
Control Treatment ◽  
Growth Stages ◽  
N Fixation ◽  
Buenos Aires Province ◽  
N Accumulation ◽  
Nodule Number ◽  
Seed Growth ◽  
Chlorimuron Ethyl

Two trials were performed in Balcarce, Argentina (37° 45' LS; 58° 18' LW) during 1993-94, to assess the effect of eight herbicides applied individually or in tank mixtures, on nodule number, nodule dry weight, seed yield and N percent in seed in soybean Asgrow 3205, inoculated with Bradyrhizobium japonicum CB 1809. Individual herbicides and doses in kg ha-1 of a.i. were metribuzin (0.48), acetochlor (0.90), metolachlor (1), flumioxazin (0.075), trifluralin (0.96), imazaquin (0.20), imazethapyr (0.10) and chlorimuron ethyl (0.0125). The mixtures were metribuzin+acetochlor (0.48+0.9), flumioxazin+acetochlor (0.075+0.9), imazaquin+acetochlor (0.2+0.9), metribuzin+metolachlor (0.48+1.92), and flumioxazin+ metolachlor (0.075+1.92). A control treatment without herbicides was included. Both trials were laid out as randomized complete blocks with four replicates, on a loam illitic thermic petrocalcic Paleudoll, 5.7% organic matter (OM), 25% clay, 30.4 cmol kg-1 CEC. Nodules were sampled at V2 (second node), V6 (sixth node) and R5 (beginning seed) growth stages. Herbicides did not significantly affect the beginning of nodulation or nodule number and mass at R5, not either grain yield or N accumulation. This indicates lack of interference between soil interacting herbicides and N fixation in the high organic matter, loam soils of SE Buenos Aires province, even though a tendency in less number and dry weight of nodules was evident at the two latter growth stages.

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